@conference{
author = "Kozarski, Maja and Vunduk, Jovana and Klaus, Anita and Špirović-Trifunović, Bojana and Lazić, Vesna and Grahovac, Vanja and Miletić, Srđan",
year = "2024",
abstract = "Tyrosinase is widely present in plants and mushrooms
and is responsible for enzymatic browning
reactions in damaged foodstuff. Accordingly, its
inhibitors act as anti-browning compounds and
have an important role in maintaining food quality.
In the cosmetic industry, inhibitors of tyrosinase
have important applications as skin-lightening
agents. Due to the ability of phenols to react
with proteins, the potential of polypore mushrooms
methanol extracts to inhibit tyrosinase was
investigated. Fresh wild-growing fruiting bodies
of the two mushroom species, namely Fomitipsis
pinicola and Ganoderma lucidum, were collected
from the Kopaonik and Avala mountains,
Republic of Serbia. Their methanol extracts were
analyzed for the total phenol content (TPC) and
phenolic profile using liquid chromatography
coupled with mass spectrometry (LC-MS/MS). The
tyrosinase inhibitory potential was determined
in the reaction solution of 46 units/mL tyrosinase
and 2.5 mM of dihydroxyphenylalanine (L-DOPA).
Results were expressed as IC50 values, the concentration
of extract required for 50% in vitro inhibition.
The results showed that TPC levels were
from 38.6 to 133.1 mg gallic acid equivalent (GAE)
of extract dry weight (DW), with F. pinicola having
the highest level. F. pinicola extract displayed
the strongest tyrosinase inhibitory activity (0.10
mg/mL) almost comparable with kojic acid (0.079
mg/mL) commonly used as a standard inhibitor
of tyrosinase. A very strong and significant correlation
between TPC and IC50 values in tyrosinase
inhibition was observed (r = - 0.96). Gallic acid
(951.12 μg/g extract DW) was found to be the
main polyphenol ingredient of F. pinicola extract.
In addition, the anti-tyrosinase activity exhibited
by investigated methanol extracts could also be
attributed to the presence of other phenolic acids
like protocatechuic, p-hydroxybenzoic, chlorogenic,
vanillic, p-coumaric, and caffeic acid. The
results of the present study suggest that methanol
extract of the polypore mushrooms F. pinicola
and G. lucidum originating from Serbia act as natural
tyrosinase inhibitors and are rich sources of
phenolic acids. These mushrooms may be a good
material for the development of anti-browning
additives as well as additives in skin-lightening
cosmeceutical formulations.",
publisher = "Serbian Society for Microbiology",
journal = "Book of Abstracts - XIII Congress of Microbiologist of Serbia, with international participation, Mikromed Regio 5, From biotechnology to human and planetary health, 4th - 6th April 2024, Belgrade, Serbia",
title = "Polyphenol profile and anti-tyrosinase potential of the polypore mushrooms Formitopsis pinicola and Ganoderma lucidum",
pages = "51-51",
url = "https://hdl.handle.net/21.15107/rcub_cer_7605"
}
