TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Jakovljević, Dragica
AU  - Jadranin, Milka
AU  - Nikšić, Miomir
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3727
AB  - The health promoting effects of hot water extracts obtained from
fruiting bodies of the commercially cultivated mushroom Agaricus bisporus
(AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW)
originating from northern Serbia are presented in this research. These abilities
were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic
acid model system, inhibition of the angiotension converting I enzyme
(ACE) that could help in the maintenance of a normal blood pressure level and
strengthening the ability of the central cholinergic neuron by inhibiting the activity
of acetylcholinesterase (AChE). Cytotoxic activities were observed
towards selected human malignant (HeLa and K562) cell lines and normal-
-human peripheral blood mononuclear cells (PBMC). GrHW contains higher
phenolics (5.9 g (100 g)-1), inhibition of LPx (EC50 = 1.07 mg mL-1), ACE
(IC50 = 0.54 mg mL-1) and AChE (IC50 = 0.37 mg mL-1), and exhibited a significant
selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL-1)
and K562 (IC50 = 0.11 mg mL-1) cells. AbHW contained higher total protein
(6.4 g (100 g)-1), carbohydrate (75.4 g (100 g)-1) and β-glucan (55.1 g (100 g)-1)
contents and induced significant proliferation of healthy PBMC from 152–116
% in the concentration range of 0.047–0.187 mg mL-1. The difference in the
biological activity of the extracts provides guidance on their use as functional
food.
AB  - У овоме раду поређен је здраствени ефекaт врелих водених екстраката добијених из плодоносних тела комерцијално узгајане јестиве гљиве Agaricus bisporus (AbHW) и самоникле врсте гљиве Ganoderma resinaceum (GrHW) из региона северне Србије. Здраствени ефекат је поређен in vitro превенцијом липидне пероксидације (LPx) у модел систему линолеинске киселине, инхибицијом ангиотензин конвертујућег ензима (ACE) који има улогу у одржавању нормалног нивоа крвног притиска и јачањем способности централних холинергичких неурона инхибицијом активности ацетилхолинестеразе (AChE).
Цитотоксична активност је праћена на хуманим ћелијама тумора грлића материц (HeLa) и ћелијама хроничне мијелоидне леукемије (K562), као и на здравим мононуклеарним ћелијама периферног крвотока (PBMC). GrHW је показао већи садржај фенолних компоненти (5,9 g (100 g)-1), већу способност инхибиције LPx (EC50 = 1,07 mg mL-1),
ACE (IC50 = 0,54 mg mL-1) и AChE (IC50 = 0,37 mg mL-1); показао је већу селективност у антитуморском дејству према HeLa (IC50 = 0,14 mg mL-1) и K562 (IC50 = 0,11 mg mL-1) ћелијама. AbHW је показао већи укупни садржај протеина (6,4 g (100 g)-1), угљених хидрата (75,4 g (100 g)-1) и β-глукана (55,1 g (100 g)-1) и значајно je стимулисао пролиферацију PBMC ћелија од 152–116 % у распону концентрација од 0,046–0,187 mg mL-1. Разлика у биолошкој активности екстраката даје смернице у њиховој примени као функционалнe хранe.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum – A comparative overview
T1  - Компаративни прглед здравствених ефеката комерцијално узгајане гљиве Agaricus bisporus и самоникле врсте гливе Ganoderma resinaceum
VL  - 85
IS  - 6
SP  - 721
EP  - 735
DO  - 10.2298/JSC190930129K
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Vunduk, Jovana and Jakovljević, Dragica and Jadranin, Milka and Nikšić, Miomir",
year = "2020",
abstract = "The health promoting effects of hot water extracts obtained from
fruiting bodies of the commercially cultivated mushroom Agaricus bisporus
(AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW)
originating from northern Serbia are presented in this research. These abilities
were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic
acid model system, inhibition of the angiotension converting I enzyme
(ACE) that could help in the maintenance of a normal blood pressure level and
strengthening the ability of the central cholinergic neuron by inhibiting the activity
of acetylcholinesterase (AChE). Cytotoxic activities were observed
towards selected human malignant (HeLa and K562) cell lines and normal-
-human peripheral blood mononuclear cells (PBMC). GrHW contains higher
phenolics (5.9 g (100 g)-1), inhibition of LPx (EC50 = 1.07 mg mL-1), ACE
(IC50 = 0.54 mg mL-1) and AChE (IC50 = 0.37 mg mL-1), and exhibited a significant
selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL-1)
and K562 (IC50 = 0.11 mg mL-1) cells. AbHW contained higher total protein
(6.4 g (100 g)-1), carbohydrate (75.4 g (100 g)-1) and β-glucan (55.1 g (100 g)-1)
contents and induced significant proliferation of healthy PBMC from 152–116
% in the concentration range of 0.047–0.187 mg mL-1. The difference in the
biological activity of the extracts provides guidance on their use as functional
food., У овоме раду поређен је здраствени ефекaт врелих водених екстраката добијених из плодоносних тела комерцијално узгајане јестиве гљиве Agaricus bisporus (AbHW) и самоникле врсте гљиве Ganoderma resinaceum (GrHW) из региона северне Србије. Здраствени ефекат је поређен in vitro превенцијом липидне пероксидације (LPx) у модел систему линолеинске киселине, инхибицијом ангиотензин конвертујућег ензима (ACE) који има улогу у одржавању нормалног нивоа крвног притиска и јачањем способности централних холинергичких неурона инхибицијом активности ацетилхолинестеразе (AChE).
Цитотоксична активност је праћена на хуманим ћелијама тумора грлића материц (HeLa) и ћелијама хроничне мијелоидне леукемије (K562), као и на здравим мононуклеарним ћелијама периферног крвотока (PBMC). GrHW је показао већи садржај фенолних компоненти (5,9 g (100 g)-1), већу способност инхибиције LPx (EC50 = 1,07 mg mL-1),
ACE (IC50 = 0,54 mg mL-1) и AChE (IC50 = 0,37 mg mL-1); показао је већу селективност у антитуморском дејству према HeLa (IC50 = 0,14 mg mL-1) и K562 (IC50 = 0,11 mg mL-1) ћелијама. AbHW је показао већи укупни садржај протеина (6,4 g (100 g)-1), угљених хидрата (75,4 g (100 g)-1) и β-глукана (55,1 g (100 g)-1) и значајно je стимулисао пролиферацију PBMC ћелија од 152–116 % у распону концентрација од 0,046–0,187 mg mL-1. Разлика у биолошкој активности екстраката даје смернице у њиховој примени као функционалнe хранe.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum – A comparative overview, Компаративни прглед здравствених ефеката комерцијално узгајане гљиве Agaricus bisporus и самоникле врсте гливе Ganoderma resinaceum",
volume = "85",
number = "6",
pages = "721-735",
doi = "10.2298/JSC190930129K"
}

Kozarski, M. S., Klaus, A., Vunduk, J., Jakovljević, D., Jadranin, M.,& Nikšić, M.. (2020). Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum – A comparative overview. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 85(6), 721-735.
https://doi.org/10.2298/JSC190930129K

Kozarski MS, Klaus A, Vunduk J, Jakovljević D, Jadranin M, Nikšić M. Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum – A comparative overview. in Journal of the Serbian Chemical Society. 2020;85(6):721-735.
doi:10.2298/JSC190930129K .

Kozarski, Maja S., Klaus, Anita, Vunduk, Jovana, Jakovljević, Dragica, Jadranin, Milka, Nikšić, Miomir, "Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum – A comparative overview" in Journal of the Serbian Chemical Society, 85, no. 6 (2020):721-735,
https://doi.org/10.2298/JSC190930129K . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Wan Abd Al Qadr Imad, Wan-Mohtar
AU  - Nikšić, Miomir
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2992
AB  - Three different polysaccharide extracts of a wild European source of the mushroom Ganoderma lucidum were screened for their free radical-blocking potential, which could strengthen the skin’s barrier function, and provide a skinlightening effect via potential inhibition of tyrosinase. The anti-collagenase and anti-elastase activities, which can help to restore skin elasticity and tensile strength, were also evaluated for the three extracts. Carbohydrates were the most abundant
components of the extracts, followed by smaller quantities of proteins, phenols and flavonoids. The glucan fraction represented between 48% and 61% of carbohydrate content in all three extracts. None of the analyzed extracts showed overt toxicity to spontaneously-transformed immortal human keratinocytes (HaCaT) at concentrations up to 2 mg/mL, and displayed superior scavenging ability on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The hot water crude polysaccharide extract (HWCP) and partially purified fraction
(HWPP) were found to be effective inhibitors of lipid peroxidation (LPx), with an almost two-fold increased inhibition of LPx compared with ascorbic acid (EC50=1.65±0.08 mg/mL), a common additive in cosmeceutical formulations and used at mg levels. Among the investigated extracts, HWCP showed the strongest inhibition potential on tyrosinase and skin extracellular matrix (ECM) degradation enzymes. These diverse functionalities indicate that G. lucidum may represent a promising source of natural cosmeceutical ingredients.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes
VL  - 71
IS  - 2
SP  - 253
EP  - 264
DO  - 10.2298/ABS181217007K
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Wan Abd Al Qadr Imad, Wan-Mohtar and Nikšić, Miomir",
year = "2019",
abstract = "Three different polysaccharide extracts of a wild European source of the mushroom Ganoderma lucidum were screened for their free radical-blocking potential, which could strengthen the skin’s barrier function, and provide a skinlightening effect via potential inhibition of tyrosinase. The anti-collagenase and anti-elastase activities, which can help to restore skin elasticity and tensile strength, were also evaluated for the three extracts. Carbohydrates were the most abundant
components of the extracts, followed by smaller quantities of proteins, phenols and flavonoids. The glucan fraction represented between 48% and 61% of carbohydrate content in all three extracts. None of the analyzed extracts showed overt toxicity to spontaneously-transformed immortal human keratinocytes (HaCaT) at concentrations up to 2 mg/mL, and displayed superior scavenging ability on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The hot water crude polysaccharide extract (HWCP) and partially purified fraction
(HWPP) were found to be effective inhibitors of lipid peroxidation (LPx), with an almost two-fold increased inhibition of LPx compared with ascorbic acid (EC50=1.65±0.08 mg/mL), a common additive in cosmeceutical formulations and used at mg levels. Among the investigated extracts, HWCP showed the strongest inhibition potential on tyrosinase and skin extracellular matrix (ECM) degradation enzymes. These diverse functionalities indicate that G. lucidum may represent a promising source of natural cosmeceutical ingredients.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes",
volume = "71",
number = "2",
pages = "253-264",
doi = "10.2298/ABS181217007K"
}

Kozarski, M. S., Klaus, A., Jakovljević, D., Todorović, N., Wan Abd Al Qadr Imad, W.,& Nikšić, M.. (2019). Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes. in Archives of Biological Sciences
Serbian Biological Society., 71(2), 253-264.
https://doi.org/10.2298/ABS181217007K

Kozarski MS, Klaus A, Jakovljević D, Todorović N, Wan Abd Al Qadr Imad W, Nikšić M. Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes. in Archives of Biological Sciences. 2019;71(2):253-264.
doi:10.2298/ABS181217007K .

Kozarski, Maja S., Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Wan Abd Al Qadr Imad, Wan-Mohtar, Nikšić, Miomir, "Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes" in Archives of Biological Sciences, 71, no. 2 (2019):253-264,
https://doi.org/10.2298/ABS181217007K . .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Vunduk, Jovana
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Nikšić, Miomir
AU  - Žižak, Željko
AU  - Vukovic, Nebojsa
AU  - Šekularac, Gavrilo
AU  - Drmanić, Saša Ž.
AU  - Bugarski, Branko
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1966
AB  - Handkea utriformis (HU), H. excipuliformis (HE) and Vascellum pratense (VP) mature fruiting bodies methanol extracts were tested for biological activities and active compounds. The extracts showed prominent radical scavenging, reducing, antioxidative and chelating abilities. Scavenging ability was correlated with phenolic (ABTS assay) or phenolic and/or sugar/beta-glucan content (DPPH). Antioxidative and Fe3+-reducing ability of VP extract was the highest, and was best correlated with flavonoid content. The same extract exhibited the best angiotensin-converting enzyme inhibitory activity. HU and HE showed selectivity toward tumor cell lines in cytotoxicity analysis. The extracts exhibited various antimicrobial activity, the best being against Listeria monocytogenes (HE, MIC-0.625 mg/mL); fatty acid content was particularly high in HU (37.25 mg/g), with linoleic acid making up more than 57% in all samples. Phenolics were present in considerable amount, as well as beta-glucans (HU, 16.67%). Although these mushrooms are inedible after autolysis process, they were still a good source of biologically active products.
PB  - Elsevier
T2  - Journal of Functional Foods
T1  - Biological potential of puffballs: A comparative analysis
VL  - 21
SP  - 36
EP  - 49
DO  - 10.1016/j.jff.2015.11.039
ER  - 

@article{
author = "Petrović, Predrag and Vunduk, Jovana and Klaus, Anita and Kozarski, Maja S. and Nikšić, Miomir and Žižak, Željko and Vukovic, Nebojsa and Šekularac, Gavrilo and Drmanić, Saša Ž. and Bugarski, Branko",
year = "2016",
abstract = "Handkea utriformis (HU), H. excipuliformis (HE) and Vascellum pratense (VP) mature fruiting bodies methanol extracts were tested for biological activities and active compounds. The extracts showed prominent radical scavenging, reducing, antioxidative and chelating abilities. Scavenging ability was correlated with phenolic (ABTS assay) or phenolic and/or sugar/beta-glucan content (DPPH). Antioxidative and Fe3+-reducing ability of VP extract was the highest, and was best correlated with flavonoid content. The same extract exhibited the best angiotensin-converting enzyme inhibitory activity. HU and HE showed selectivity toward tumor cell lines in cytotoxicity analysis. The extracts exhibited various antimicrobial activity, the best being against Listeria monocytogenes (HE, MIC-0.625 mg/mL); fatty acid content was particularly high in HU (37.25 mg/g), with linoleic acid making up more than 57% in all samples. Phenolics were present in considerable amount, as well as beta-glucans (HU, 16.67%). Although these mushrooms are inedible after autolysis process, they were still a good source of biologically active products.",
publisher = "Elsevier",
journal = "Journal of Functional Foods",
title = "Biological potential of puffballs: A comparative analysis",
volume = "21",
pages = "36-49",
doi = "10.1016/j.jff.2015.11.039"
}

Petrović, P., Vunduk, J., Klaus, A., Kozarski, M. S., Nikšić, M., Žižak, Ž., Vukovic, N., Šekularac, G., Drmanić, S. Ž.,& Bugarski, B.. (2016). Biological potential of puffballs: A comparative analysis. in Journal of Functional Foods
Elsevier., 21, 36-49.
https://doi.org/10.1016/j.jff.2015.11.039

Petrović P, Vunduk J, Klaus A, Kozarski MS, Nikšić M, Žižak Ž, Vukovic N, Šekularac G, Drmanić SŽ, Bugarski B. Biological potential of puffballs: A comparative analysis. in Journal of Functional Foods. 2016;21:36-49.
doi:10.1016/j.jff.2015.11.039 .

Petrović, Predrag, Vunduk, Jovana, Klaus, Anita, Kozarski, Maja S., Nikšić, Miomir, Žižak, Željko, Vukovic, Nebojsa, Šekularac, Gavrilo, Drmanić, Saša Ž., Bugarski, Branko, "Biological potential of puffballs: A comparative analysis" in Journal of Functional Foods, 21 (2016):36-49,
https://doi.org/10.1016/j.jff.2015.11.039 . .

TY  - JOUR
AU  - Pecic, Sonja
AU  - Nikićević, Ninoslav
AU  - Veljović, Mile
AU  - Jadranin, Milka
AU  - Tešević, Vele
AU  - Belovic, Miona
AU  - Nikšić, Miomir
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1935
AB  - Ganoderma lucidum is one of the five major medicinal mushrooms. In Asian countries, alcoholic beverages with Ganoderma are traditionally produced and sold in local markets as a symbol of healthy products. The aim of this study was to examine the possibility of producing brandy enhanced with this mushroom and to investigate the influence of extraction parameters (time, concentration) on color, total phenolic content, antioxidant capacity, sensory characteristics and the composition and content of triterpenoid acids within the brandy. HPLC-DAD/ESI-ToF-MS analysis was used to identify triterpenoid acids. In brandy samples, 15 triterpenoid acids were determined, with the total content in the range of 2.63-4.06 mg/100 mg. In these samples, the most commonly detected triterpenoid acid was ganoderic acid A. In our study, the total phenolic content of analyzed samples ranged from 34.07 to 118.1 mg/L GAE. The color and sensory characteristics of analyzed brandies were significantly improved in comparison with samples without G. lucidum. The obtained samples represent an interesting new product for market worldwide with improved antioxidant capacity.
PB  - Assoc Chemical Eng, Belgrade
T2  - Chemical Industry and Chemical Engineering Quarterly / CICEQ
T1  - The influence of extraction parameters on physicochemical properties of special grain brandies with ganoderma lucidum
VL  - 22
IS  - 2
SP  - 181
EP  - 189
DO  - 10.2298/CICEQ150426033P
ER  - 

@article{
author = "Pecic, Sonja and Nikićević, Ninoslav and Veljović, Mile and Jadranin, Milka and Tešević, Vele and Belovic, Miona and Nikšić, Miomir",
year = "2016",
abstract = "Ganoderma lucidum is one of the five major medicinal mushrooms. In Asian countries, alcoholic beverages with Ganoderma are traditionally produced and sold in local markets as a symbol of healthy products. The aim of this study was to examine the possibility of producing brandy enhanced with this mushroom and to investigate the influence of extraction parameters (time, concentration) on color, total phenolic content, antioxidant capacity, sensory characteristics and the composition and content of triterpenoid acids within the brandy. HPLC-DAD/ESI-ToF-MS analysis was used to identify triterpenoid acids. In brandy samples, 15 triterpenoid acids were determined, with the total content in the range of 2.63-4.06 mg/100 mg. In these samples, the most commonly detected triterpenoid acid was ganoderic acid A. In our study, the total phenolic content of analyzed samples ranged from 34.07 to 118.1 mg/L GAE. The color and sensory characteristics of analyzed brandies were significantly improved in comparison with samples without G. lucidum. The obtained samples represent an interesting new product for market worldwide with improved antioxidant capacity.",
publisher = "Assoc Chemical Eng, Belgrade",
journal = "Chemical Industry and Chemical Engineering Quarterly / CICEQ",
title = "The influence of extraction parameters on physicochemical properties of special grain brandies with ganoderma lucidum",
volume = "22",
number = "2",
pages = "181-189",
doi = "10.2298/CICEQ150426033P"
}

Pecic, S., Nikićević, N., Veljović, M., Jadranin, M., Tešević, V., Belovic, M.,& Nikšić, M.. (2016). The influence of extraction parameters on physicochemical properties of special grain brandies with ganoderma lucidum. in Chemical Industry and Chemical Engineering Quarterly / CICEQ
Assoc Chemical Eng, Belgrade., 22(2), 181-189.
https://doi.org/10.2298/CICEQ150426033P

Pecic S, Nikićević N, Veljović M, Jadranin M, Tešević V, Belovic M, Nikšić M. The influence of extraction parameters on physicochemical properties of special grain brandies with ganoderma lucidum. in Chemical Industry and Chemical Engineering Quarterly / CICEQ. 2016;22(2):181-189.
doi:10.2298/CICEQ150426033P .

Pecic, Sonja, Nikićević, Ninoslav, Veljović, Mile, Jadranin, Milka, Tešević, Vele, Belovic, Miona, Nikšić, Miomir, "The influence of extraction parameters on physicochemical properties of special grain brandies with ganoderma lucidum" in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 22, no. 2 (2016):181-189,
https://doi.org/10.2298/CICEQ150426033P . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Žižak, Željko
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1666
AB  - The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms
VL  - 6
IS  - 6
SP  - 1875
EP  - 1886
DO  - 10.1039/c5fo00312a
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Vunduk, Jovana and Žižak, Željko and Nikšić, Miomir and Jakovljević, Dragica and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms",
volume = "6",
number = "6",
pages = "1875-1886",
doi = "10.1039/c5fo00312a"
}

Kozarski, M. S., Klaus, A., Vunduk, J., Žižak, Ž., Nikšić, M., Jakovljević, D., Vrvić, M.,& Van Griensven, L. J. L. D.. (2015). Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. in Food & Function
Royal Soc Chemistry, Cambridge., 6(6), 1875-1886.
https://doi.org/10.1039/c5fo00312a

Kozarski MS, Klaus A, Vunduk J, Žižak Ž, Nikšić M, Jakovljević D, Vrvić M, Van Griensven LJLD. Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. in Food & Function. 2015;6(6):1875-1886.
doi:10.1039/c5fo00312a .

Kozarski, Maja S., Klaus, Anita, Vunduk, Jovana, Žižak, Željko, Nikšić, Miomir, Jakovljević, Dragica, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms" in Food & Function, 6, no. 6 (2015):1875-1886,
https://doi.org/10.1039/c5fo00312a . .

TY  - JOUR
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Vunduk, Jovana
AU  - Todorović, Nina
AU  - Jakovljević, Dragica
AU  - Žižak, Željko
AU  - Pavlović, Vladimir B.
AU  - Levic, Steva
AU  - Nikšić, Miomir
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1761
AB  - Partially purified polysaccharides (FP) and hot alkali extract (FNa) obtained from fruiting bodies of the wild basidiomycete Grifola frondosa were examined for their antimicrobial, antioxidant and cytotoxic activity. The structural properties of FP and FNa samples were investigated by FT-IR and high resolution 1H- and 13C-NMR spectroscopy. From a group of various G- and G+ bacteria the antibacterial effects were highest against the G + B. cereus. FNa was the better antioxidant shown by the lower EC50 values of DPPH scavenging ability, ferric-reducing antioxidant power and ferrous ion-chelating ability. Ferric-reducing antioxidant power and ferrous ion-chelating ability were mostly linked to total polysaccharides, total- and beta-glucan content, as well as total protein content. Both extracts displayed a moderate dose dependent antiproliferative action towards malignant human breast cancer MDA-MB-453, cervical adenocarcinoma HeLa and myelogenous leukemia K562 cells not observed in the non cancer derived MRC-5 fibroblasts. The highest effect was found in HeLa cells for FP extract. The mean diameter of Ca-alginate bead loading FP was 960.7 mu m while the mean diameter of beads encapsulating FNa extract was 1051.7 mu m.
PB  - Elsevier
T2  - Food Research International
T1  - Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa
VL  - 67
SP  - 272
EP  - 283
DO  - 10.1016/j.foodres.2014.11.035
ER  - 

@article{
author = "Klaus, Anita and Kozarski, Maja S. and Vunduk, Jovana and Todorović, Nina and Jakovljević, Dragica and Žižak, Željko and Pavlović, Vladimir B. and Levic, Steva and Nikšić, Miomir and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "Partially purified polysaccharides (FP) and hot alkali extract (FNa) obtained from fruiting bodies of the wild basidiomycete Grifola frondosa were examined for their antimicrobial, antioxidant and cytotoxic activity. The structural properties of FP and FNa samples were investigated by FT-IR and high resolution 1H- and 13C-NMR spectroscopy. From a group of various G- and G+ bacteria the antibacterial effects were highest against the G + B. cereus. FNa was the better antioxidant shown by the lower EC50 values of DPPH scavenging ability, ferric-reducing antioxidant power and ferrous ion-chelating ability. Ferric-reducing antioxidant power and ferrous ion-chelating ability were mostly linked to total polysaccharides, total- and beta-glucan content, as well as total protein content. Both extracts displayed a moderate dose dependent antiproliferative action towards malignant human breast cancer MDA-MB-453, cervical adenocarcinoma HeLa and myelogenous leukemia K562 cells not observed in the non cancer derived MRC-5 fibroblasts. The highest effect was found in HeLa cells for FP extract. The mean diameter of Ca-alginate bead loading FP was 960.7 mu m while the mean diameter of beads encapsulating FNa extract was 1051.7 mu m.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa",
volume = "67",
pages = "272-283",
doi = "10.1016/j.foodres.2014.11.035"
}

Klaus, A., Kozarski, M. S., Vunduk, J., Todorović, N., Jakovljević, D., Žižak, Ž., Pavlović, V. B., Levic, S., Nikšić, M.,& Van Griensven, L. J. L. D.. (2015). Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa. in Food Research International
Elsevier., 67, 272-283.
https://doi.org/10.1016/j.foodres.2014.11.035

Klaus A, Kozarski MS, Vunduk J, Todorović N, Jakovljević D, Žižak Ž, Pavlović VB, Levic S, Nikšić M, Van Griensven LJLD. Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa. in Food Research International. 2015;67:272-283.
doi:10.1016/j.foodres.2014.11.035 .

Klaus, Anita, Kozarski, Maja S., Vunduk, Jovana, Todorović, Nina, Jakovljević, Dragica, Žižak, Željko, Pavlović, Vladimir B., Levic, Steva, Nikšić, Miomir, Van Griensven, Leo J. L. D., "Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa" in Food Research International, 67 (2015):272-283,
https://doi.org/10.1016/j.foodres.2014.11.035 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Vunduk, Jovana
AU  - Petrović, Predrag
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1826
AB  - Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.
PB  - MDPI
T2  - Molecules
T1  - Antioxidants of Edible Mushrooms
VL  - 20
IS  - 10
SP  - 19489
EP  - 19525
DO  - 10.3390/molecules201019489
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Vunduk, Jovana and Petrović, Predrag and Nikšić, Miomir and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.",
publisher = "MDPI",
journal = "Molecules",
title = "Antioxidants of Edible Mushrooms",
volume = "20",
number = "10",
pages = "19489-19525",
doi = "10.3390/molecules201019489"
}

Kozarski, M. S., Klaus, A., Jakovljević, D., Todorović, N., Vunduk, J., Petrović, P., Nikšić, M., Vrvić, M.,& Van Griensven, L. J. L. D.. (2015). Antioxidants of Edible Mushrooms. in Molecules
MDPI., 20(10), 19489-19525.
https://doi.org/10.3390/molecules201019489

Kozarski MS, Klaus A, Jakovljević D, Todorović N, Vunduk J, Petrović P, Nikšić M, Vrvić M, Van Griensven LJLD. Antioxidants of Edible Mushrooms. in Molecules. 2015;20(10):19489-19525.
doi:10.3390/molecules201019489 .

Kozarski, Maja S., Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Vunduk, Jovana, Petrović, Predrag, Nikšić, Miomir, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Antioxidants of Edible Mushrooms" in Molecules, 20, no. 10 (2015):19489-19525,
https://doi.org/10.3390/molecules201019489 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1395
AB  - Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.
PB  - Elsevier
T2  - Food Research International
T1  - Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification
VL  - 64
SP  - 53
EP  - 64
DO  - 10.1016/j.foodres.2014.05.075
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Nikšić, Miomir and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2014",
abstract = "Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification",
volume = "64",
pages = "53-64",
doi = "10.1016/j.foodres.2014.05.075"
}

Kozarski, M. S., Klaus, A., Jakovljević, D., Todorović, N., Nikšić, M., Vrvić, M.,& Van Griensven, L. J. L. D.. (2014). Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. in Food Research International
Elsevier., 64, 53-64.
https://doi.org/10.1016/j.foodres.2014.05.075

Kozarski MS, Klaus A, Jakovljević D, Todorović N, Nikšić M, Vrvić M, Van Griensven LJLD. Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. in Food Research International. 2014;64:53-64.
doi:10.1016/j.foodres.2014.05.075 .

Kozarski, Maja S., Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Nikšić, Miomir, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification" in Food Research International, 64 (2014):53-64,
https://doi.org/10.1016/j.foodres.2014.05.075 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Van Griensven, Leo J. L. D.
AU  - Vrvić, Miroslav
AU  - Jakovljević, Dragica
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1587
AB  - The fungal polysaccharides attract a lot of attention due to their multiple challenging biological properties, such as: anti-tumor, anti-viral, anticomplementary, anticoagulant, hypolipidemic, immunomodulatory and immune-stimulatory activities, which all together make them suitable for application in many quite distinctive areas, such as food industry, biomedicine, cosmetology, agriculture, environmental protection and waste water management. This article presents results with respect to biological properties, structure and procedures related to the isolation and activation of polysaccharides of higher fungi. It is considered and presented along with a review of the critical antioxidative activity and possible influence of the structural composition of polysaccharide extracts (isolated from these higher fungi) upon their antioxidative properties.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Polysaccharides of higher fungi: biological role, structure and antioxidative activity
VL  - 68
IS  - 3
SP  - 305
EP  - 320
DO  - 10.2298/HEMIND121114056K
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Nikšić, Miomir and Van Griensven, Leo J. L. D. and Vrvić, Miroslav and Jakovljević, Dragica",
year = "2014",
abstract = "The fungal polysaccharides attract a lot of attention due to their multiple challenging biological properties, such as: anti-tumor, anti-viral, anticomplementary, anticoagulant, hypolipidemic, immunomodulatory and immune-stimulatory activities, which all together make them suitable for application in many quite distinctive areas, such as food industry, biomedicine, cosmetology, agriculture, environmental protection and waste water management. This article presents results with respect to biological properties, structure and procedures related to the isolation and activation of polysaccharides of higher fungi. It is considered and presented along with a review of the critical antioxidative activity and possible influence of the structural composition of polysaccharide extracts (isolated from these higher fungi) upon their antioxidative properties.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Polysaccharides of higher fungi: biological role, structure and antioxidative activity",
volume = "68",
number = "3",
pages = "305-320",
doi = "10.2298/HEMIND121114056K"
}

Kozarski, M. S., Klaus, A., Nikšić, M., Van Griensven, L. J. L. D., Vrvić, M.,& Jakovljević, D.. (2014). Polysaccharides of higher fungi: biological role, structure and antioxidative activity. in Hemijska industrija
Association of Chemical Engineers of Serbia., 68(3), 305-320.
https://doi.org/10.2298/HEMIND121114056K

Kozarski MS, Klaus A, Nikšić M, Van Griensven LJLD, Vrvić M, Jakovljević D. Polysaccharides of higher fungi: biological role, structure and antioxidative activity. in Hemijska industrija. 2014;68(3):305-320.
doi:10.2298/HEMIND121114056K .

Kozarski, Maja S., Klaus, Anita, Nikšić, Miomir, Van Griensven, Leo J. L. D., Vrvić, Miroslav, Jakovljević, Dragica, "Polysaccharides of higher fungi: biological role, structure and antioxidative activity" in Hemijska industrija, 68, no. 3 (2014):305-320,
https://doi.org/10.2298/HEMIND121114056K . .

TY  - JOUR
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Stefanoska, Ivana
AU  - Van Griensven, Leo J. L. D.
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1196
AB  - Hot water extract (LN), partially purified polysaccharides (LP) and hot alkali extracted polysaccharides (LNa) obtained from fruiting bodies of the wild basidiomycete Laetiporus sulphureus were examined for their antioxidant activities. LNa was the most active antioxidant, as shown by the median effective concentrations (EC50 values) of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity (0.5 +/- 0.2 mg/ml), reducing power (4.0 +/- 0.3 mg/ml) and ferrous ion-chelating ability (1.5 +/- 0.1 mg/ml). LNa contained the highest level of alpha-glucan (17.3 +/- 1.2 g/100 g dw), whereas LP contained mostly beta-glucans (66.8 +/- 1.3 g/100 g dw). The prevalent monosaccharide in all extracts was glucose. The EC50 values of all three antioxidant activity assays were well-correlated with the alpha-glucan content. Strong and significant correlation was found between total phenolic compounds and DPPH scavenging ability and also reducing power. The three investigated extracts (at concentrations of 0.1-10 mg/ml) were not toxic to HTR-8/SVneo trophoblast cell line.
PB  - Informa Healthcare, London
T2  - International Journal of Food Sciences and Nutrition
T1  - The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants
VL  - 64
IS  - 5
SP  - 599
EP  - 610
DO  - 10.3109/09637486.2012.759190
ER  - 

@article{
author = "Klaus, Anita and Kozarski, Maja S. and Nikšić, Miomir and Jakovljević, Dragica and Todorović, Nina and Stefanoska, Ivana and Van Griensven, Leo J. L. D.",
year = "2013",
abstract = "Hot water extract (LN), partially purified polysaccharides (LP) and hot alkali extracted polysaccharides (LNa) obtained from fruiting bodies of the wild basidiomycete Laetiporus sulphureus were examined for their antioxidant activities. LNa was the most active antioxidant, as shown by the median effective concentrations (EC50 values) of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity (0.5 +/- 0.2 mg/ml), reducing power (4.0 +/- 0.3 mg/ml) and ferrous ion-chelating ability (1.5 +/- 0.1 mg/ml). LNa contained the highest level of alpha-glucan (17.3 +/- 1.2 g/100 g dw), whereas LP contained mostly beta-glucans (66.8 +/- 1.3 g/100 g dw). The prevalent monosaccharide in all extracts was glucose. The EC50 values of all three antioxidant activity assays were well-correlated with the alpha-glucan content. Strong and significant correlation was found between total phenolic compounds and DPPH scavenging ability and also reducing power. The three investigated extracts (at concentrations of 0.1-10 mg/ml) were not toxic to HTR-8/SVneo trophoblast cell line.",
publisher = "Informa Healthcare, London",
journal = "International Journal of Food Sciences and Nutrition",
title = "The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants",
volume = "64",
number = "5",
pages = "599-610",
doi = "10.3109/09637486.2012.759190"
}

Klaus, A., Kozarski, M. S., Nikšić, M., Jakovljević, D., Todorović, N., Stefanoska, I.,& Van Griensven, L. J. L. D.. (2013). The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants. in International Journal of Food Sciences and Nutrition
Informa Healthcare, London., 64(5), 599-610.
https://doi.org/10.3109/09637486.2012.759190

Klaus A, Kozarski MS, Nikšić M, Jakovljević D, Todorović N, Stefanoska I, Van Griensven LJLD. The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants. in International Journal of Food Sciences and Nutrition. 2013;64(5):599-610.
doi:10.3109/09637486.2012.759190 .

Klaus, Anita, Kozarski, Maja S., Nikšić, Miomir, Jakovljević, Dragica, Todorović, Nina, Stefanoska, Ivana, Van Griensven, Leo J. L. D., "The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants" in International Journal of Food Sciences and Nutrition, 64, no. 5 (2013):599-610,
https://doi.org/10.3109/09637486.2012.759190 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav
AU  - Todorović, Nina
AU  - Jakovljević, Dragica
AU  - Van Griensven, Leo J. L. D.
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/987
AB  - Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts were the strongest DPPH scavengers, i.e. EC50  LT  0.1 mg/mL. G. applanatum extract had the highest chelating ability of ferrous ion (EC50 = 3.58 mg/mL), inhibition of lipid peroxidation (EC50 = 2.07 mg/mL) and had the highest reducing power, EC50 = 0.18. All four polysaccharide extracts showed a strong correlation between the reducing power and the total amount of phenols and alpha-glucans: no such correlation was found between the reducing power and the amount of total polysaccharides and proteins. The extracts contained a mixture/complex of polysaccharides, proteins and polyphenols, still present after hot water treatment, ethanol precipitation and dialysis. Glucose was the prevalent monosaccharide of all polysaccharides and samples contained both alpha- and beta-glucans. Total glucan contents varied considerably, i.e. from 35% for G. applanatum to 83% for G. lucidum. Uronic acid was found in the G. applanatum polysaccharides. Fourier transform infrared (FT-IR) analysis showed strong aromatic and ester peaks next to the different sugar and polysaccharide signals.
PB  - Academic Press Inc.
T2  - Journal of Food Composition and Analysis
T1  - Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor
VL  - 26
IS  - 1-2
SP  - 144
EP  - 153
DO  - 10.1016/j.jfca.2012.02.004
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Nikšić, Miomir and Vrvić, Miroslav and Todorović, Nina and Jakovljević, Dragica and Van Griensven, Leo J. L. D.",
year = "2012",
abstract = "Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts were the strongest DPPH scavengers, i.e. EC50  LT  0.1 mg/mL. G. applanatum extract had the highest chelating ability of ferrous ion (EC50 = 3.58 mg/mL), inhibition of lipid peroxidation (EC50 = 2.07 mg/mL) and had the highest reducing power, EC50 = 0.18. All four polysaccharide extracts showed a strong correlation between the reducing power and the total amount of phenols and alpha-glucans: no such correlation was found between the reducing power and the amount of total polysaccharides and proteins. The extracts contained a mixture/complex of polysaccharides, proteins and polyphenols, still present after hot water treatment, ethanol precipitation and dialysis. Glucose was the prevalent monosaccharide of all polysaccharides and samples contained both alpha- and beta-glucans. Total glucan contents varied considerably, i.e. from 35% for G. applanatum to 83% for G. lucidum. Uronic acid was found in the G. applanatum polysaccharides. Fourier transform infrared (FT-IR) analysis showed strong aromatic and ester peaks next to the different sugar and polysaccharide signals.",
publisher = "Academic Press Inc.",
journal = "Journal of Food Composition and Analysis",
title = "Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor",
volume = "26",
number = "1-2",
pages = "144-153",
doi = "10.1016/j.jfca.2012.02.004"
}

Kozarski, M. S., Klaus, A., Nikšić, M., Vrvić, M., Todorović, N., Jakovljević, D.,& Van Griensven, L. J. L. D.. (2012). Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. in Journal of Food Composition and Analysis
Academic Press Inc.., 26(1-2), 144-153.
https://doi.org/10.1016/j.jfca.2012.02.004

Kozarski MS, Klaus A, Nikšić M, Vrvić M, Todorović N, Jakovljević D, Van Griensven LJLD. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. in Journal of Food Composition and Analysis. 2012;26(1-2):144-153.
doi:10.1016/j.jfca.2012.02.004 .

Kozarski, Maja S., Klaus, Anita, Nikšić, Miomir, Vrvić, Miroslav, Todorović, Nina, Jakovljević, Dragica, Van Griensven, Leo J. L. D., "Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor" in Journal of Food Composition and Analysis, 26, no. 1-2 (2012):144-153,
https://doi.org/10.1016/j.jfca.2012.02.004 . .

TY  - CONF
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Vrvić, Miroslav
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1148
AB  - Fruiting bodies of some wild and cultivatable mushrooms contain medicinal compounds which are being used in traditional medicines and cosmetics. Champignon mushroom (Agaricus bisporus) is the most widely cultivated species of edible mushroom worldwide. This paper focuses on antioxidant and antimicrobial importance of A bisporus. Water-soluble polysaccharide-enriched fraction was isolated from the dry carpophores of Agaricus bisporus. Antioxidant activities were investigated using in vitro assay systems: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and chelating ability on ferrous ions. Antimicrobial activity was tested against Gram positive and Gram negative bacteria in vitro by disk diffusion method in order to determine the zones of inhibition. At concentrations of 0.1-10 mg/ml, the scavenging abilities of A bisporus ranged between 12.3-75.5 %. The radical scavenging ability of the positive controls-tocopherol and ascorbic acid, at the concentrations of 0.1-20 mg/ml, were between 79.9-80.8 and 80.6-91.1 %, respectively. Polysaccharide extract from A bisporus showed steadily increasing chelating ability as concentrations increased to 88.2 % at 20 mg/ml. The chelating ability of the citric acid was between 7.2-10.7 %, at the concentrations of 0.1-20 mg/ml. The study of antimicrobial potential of polysaccharide extract showed more potent activity against Gram-positive Enterococcus faecalis ATCC 49532 (26.7 ± 0.2 mm), Bacillus cereus 10876 (27.5 ± 0.4 mm), Geobacillus stearothermophylus ATCC 7953 (22.8 ± 0.3 mm) than Gram-negative bacteria Pseudomonas aeruginosa ATCC 35032 (10.4 ± 0.6 mm), Proteus hauseri ATCC 13315 (12.1 ± 0.1 mm) Escherichia coli (0157:H7) 35150 (12.7 ± 0.4 mm) with exception of Klebsiella pneumoniae ATCC 27736 (22.3 ± 0.2 mm).
C3  - CEFood 2012 - Proceedings of 6th Central European Congress on Food
T1  - Antioxidant and antimicrobial potentials of champignon mushroom
SP  - 144
EP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_cer_1148
ER  - 

@conference{
author = "Kozarski, Maja S. and Klaus, Anita and Nikšić, Miomir and Jakovljević, Dragica and Vrvić, Miroslav",
year = "2012",
abstract = "Fruiting bodies of some wild and cultivatable mushrooms contain medicinal compounds which are being used in traditional medicines and cosmetics. Champignon mushroom (Agaricus bisporus) is the most widely cultivated species of edible mushroom worldwide. This paper focuses on antioxidant and antimicrobial importance of A bisporus. Water-soluble polysaccharide-enriched fraction was isolated from the dry carpophores of Agaricus bisporus. Antioxidant activities were investigated using in vitro assay systems: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and chelating ability on ferrous ions. Antimicrobial activity was tested against Gram positive and Gram negative bacteria in vitro by disk diffusion method in order to determine the zones of inhibition. At concentrations of 0.1-10 mg/ml, the scavenging abilities of A bisporus ranged between 12.3-75.5 %. The radical scavenging ability of the positive controls-tocopherol and ascorbic acid, at the concentrations of 0.1-20 mg/ml, were between 79.9-80.8 and 80.6-91.1 %, respectively. Polysaccharide extract from A bisporus showed steadily increasing chelating ability as concentrations increased to 88.2 % at 20 mg/ml. The chelating ability of the citric acid was between 7.2-10.7 %, at the concentrations of 0.1-20 mg/ml. The study of antimicrobial potential of polysaccharide extract showed more potent activity against Gram-positive Enterococcus faecalis ATCC 49532 (26.7 ± 0.2 mm), Bacillus cereus 10876 (27.5 ± 0.4 mm), Geobacillus stearothermophylus ATCC 7953 (22.8 ± 0.3 mm) than Gram-negative bacteria Pseudomonas aeruginosa ATCC 35032 (10.4 ± 0.6 mm), Proteus hauseri ATCC 13315 (12.1 ± 0.1 mm) Escherichia coli (0157:H7) 35150 (12.7 ± 0.4 mm) with exception of Klebsiella pneumoniae ATCC 27736 (22.3 ± 0.2 mm).",
journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food",
title = "Antioxidant and antimicrobial potentials of champignon mushroom",
pages = "144-149",
url = "https://hdl.handle.net/21.15107/rcub_cer_1148"
}

Kozarski, M. S., Klaus, A., Nikšić, M., Jakovljević, D.,& Vrvić, M.. (2012). Antioxidant and antimicrobial potentials of champignon mushroom. in CEFood 2012 - Proceedings of 6th Central European Congress on Food, 144-149.
https://hdl.handle.net/21.15107/rcub_cer_1148

Kozarski MS, Klaus A, Nikšić M, Jakovljević D, Vrvić M. Antioxidant and antimicrobial potentials of champignon mushroom. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:144-149.
https://hdl.handle.net/21.15107/rcub_cer_1148 .

Kozarski, Maja S., Klaus, Anita, Nikšić, Miomir, Jakovljević, Dragica, Vrvić, Miroslav, "Antioxidant and antimicrobial potentials of champignon mushroom" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):144-149,
https://hdl.handle.net/21.15107/rcub_cer_1148 .

TY  - JOUR
AU  - Pecic, Sonja
AU  - Veljović, Mile
AU  - Despotovic, S.
AU  - Leskosek-Cukalovic, I.
AU  - Jadranin, Milka
AU  - Tešević, Vele
AU  - Nikšić, Miomir
AU  - Nikićević, Ninoslav
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1060
AB  - ljivovica and Prepeenica are traditional Serbian distillated beverages made from varieties of plum, of the genus Prunus. The ageing in wooden casks not only improves the sensory characteristics, but also changes the content of total polyphenols and contributes to the other important health properties, such as the increase of their antioxidant capacity. In this study, home-made brandy (ljivovica and Prepeenica) samples were used, which matured in casks during the time period from 10 to 47 years. The aim of this investigation was to examine the influence of the ageing parameters (time period and type of wooden casks) and raw material (plum cultivars) on the total polyphenol content, amount of certain phenol compounds, sensory properties, colour and antioxidant properties in matured brandies. The individual phenol components were determinated using HPLC system equipped with diode array detector, while the total phenol content and antioxidant activity was estimated using spectrophotometric methods (Folin-Ciocalteu, DPPH, FRAP and TEAC). Total polyphenol content (TPC) of aged Prepeenica samples was ranged from 230.26 to 890.26 mg/L gallic acid equivalents (GAE mg/L), whereas the TPC of ljivovica sample was 110.38 mg/L. Antioxidant capacity of analysed Prepeenica samples and ljivovica sample was in strong correlation with TPC. Total sensory score of all samples was very high and varied between 18.2 and 19.8. The obtained results have shown that the benefit of longer maturation on sensory characteristics is well recognized, but the improvement has some limitation.
PB  - Springer, New York
T2  - European Food Research and Technology
T1  - Effect of maturation conditions on sensory and antioxidant properties of old Serbian plum brandies
VL  - 235
IS  - 3
SP  - 479
EP  - 487
DO  - 10.1007/s00217-012-1775-y
ER  - 

@article{
author = "Pecic, Sonja and Veljović, Mile and Despotovic, S. and Leskosek-Cukalovic, I. and Jadranin, Milka and Tešević, Vele and Nikšić, Miomir and Nikićević, Ninoslav",
year = "2012",
abstract = "ljivovica and Prepeenica are traditional Serbian distillated beverages made from varieties of plum, of the genus Prunus. The ageing in wooden casks not only improves the sensory characteristics, but also changes the content of total polyphenols and contributes to the other important health properties, such as the increase of their antioxidant capacity. In this study, home-made brandy (ljivovica and Prepeenica) samples were used, which matured in casks during the time period from 10 to 47 years. The aim of this investigation was to examine the influence of the ageing parameters (time period and type of wooden casks) and raw material (plum cultivars) on the total polyphenol content, amount of certain phenol compounds, sensory properties, colour and antioxidant properties in matured brandies. The individual phenol components were determinated using HPLC system equipped with diode array detector, while the total phenol content and antioxidant activity was estimated using spectrophotometric methods (Folin-Ciocalteu, DPPH, FRAP and TEAC). Total polyphenol content (TPC) of aged Prepeenica samples was ranged from 230.26 to 890.26 mg/L gallic acid equivalents (GAE mg/L), whereas the TPC of ljivovica sample was 110.38 mg/L. Antioxidant capacity of analysed Prepeenica samples and ljivovica sample was in strong correlation with TPC. Total sensory score of all samples was very high and varied between 18.2 and 19.8. The obtained results have shown that the benefit of longer maturation on sensory characteristics is well recognized, but the improvement has some limitation.",
publisher = "Springer, New York",
journal = "European Food Research and Technology",
title = "Effect of maturation conditions on sensory and antioxidant properties of old Serbian plum brandies",
volume = "235",
number = "3",
pages = "479-487",
doi = "10.1007/s00217-012-1775-y"
}

Pecic, S., Veljović, M., Despotovic, S., Leskosek-Cukalovic, I., Jadranin, M., Tešević, V., Nikšić, M.,& Nikićević, N.. (2012). Effect of maturation conditions on sensory and antioxidant properties of old Serbian plum brandies. in European Food Research and Technology
Springer, New York., 235(3), 479-487.
https://doi.org/10.1007/s00217-012-1775-y

Pecic S, Veljović M, Despotovic S, Leskosek-Cukalovic I, Jadranin M, Tešević V, Nikšić M, Nikićević N. Effect of maturation conditions on sensory and antioxidant properties of old Serbian plum brandies. in European Food Research and Technology. 2012;235(3):479-487.
doi:10.1007/s00217-012-1775-y .

Pecic, Sonja, Veljović, Mile, Despotovic, S., Leskosek-Cukalovic, I., Jadranin, Milka, Tešević, Vele, Nikšić, Miomir, Nikićević, Ninoslav, "Effect of maturation conditions on sensory and antioxidant properties of old Serbian plum brandies" in European Food Research and Technology, 235, no. 3 (2012):479-487,
https://doi.org/10.1007/s00217-012-1775-y . .

TY  - JOUR
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Van Griensven, Leo J. L. D.
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/853
AB  - Antioxidant properties properties of hot water extract (HWE), hot water extracted polysaccharides (HWP) and hot alkali extracted polysaccharides (HWAE) were obtained from fruiting bodies of the wild basidiomycete Schizophyllum commune. All extracts contained both alpha- and beta-glucans as determined by Megazyme beta-glucan assay kit. Glucose was found by TLC and NMR to be the prevalent monosaccharide in all extracts. Total phenol contents were in descending order of HWP approximate to HWE > HWAE. Median effective concentrations (EC(50) values) of antioxidant activities were 8.3 +/- 0.1, 6.9 +/- 0.0 and 8.9 +/- 0.1 mg/mL, and of the DPPH scavenging activity 0.8 +/- 0.0, 0.6 +/- 0.0 and 1.8 +/- 0.0 mg/mL, for HWE, HWP and HWAE, respectively. EC(50) values of reducing power were 7.6 +/- 0.1, 7.9 +/- 0.0 and 12.5 +/- 0.1 mg/mL, whereas those of the chelating abilities on ferrous ions were 3.1 +/- 0.0, 4.6 +/- 0.1 and 4.9 +/- 0.1 mg/mL The EC(50) values of the antioxidant activity, of the DPPH scavenging, and of the reducing power were correlated with total polysaccharide as well as with total phenol content. The antioxidant activities of all the extracts may be caused by both polysaccharides and polyphenols or by a complex of both.
PB  - Elsevier
T2  - Lwt-Food Science and Technology
T1  - Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune
VL  - 44
IS  - 10
SP  - 2005
EP  - 2011
DO  - 10.1016/j.lwt.2011.05.010
ER  - 

@article{
author = "Klaus, Anita and Kozarski, Maja S. and Nikšić, Miomir and Jakovljević, Dragica and Todorović, Nina and Van Griensven, Leo J. L. D.",
year = "2011",
abstract = "Antioxidant properties properties of hot water extract (HWE), hot water extracted polysaccharides (HWP) and hot alkali extracted polysaccharides (HWAE) were obtained from fruiting bodies of the wild basidiomycete Schizophyllum commune. All extracts contained both alpha- and beta-glucans as determined by Megazyme beta-glucan assay kit. Glucose was found by TLC and NMR to be the prevalent monosaccharide in all extracts. Total phenol contents were in descending order of HWP approximate to HWE > HWAE. Median effective concentrations (EC(50) values) of antioxidant activities were 8.3 +/- 0.1, 6.9 +/- 0.0 and 8.9 +/- 0.1 mg/mL, and of the DPPH scavenging activity 0.8 +/- 0.0, 0.6 +/- 0.0 and 1.8 +/- 0.0 mg/mL, for HWE, HWP and HWAE, respectively. EC(50) values of reducing power were 7.6 +/- 0.1, 7.9 +/- 0.0 and 12.5 +/- 0.1 mg/mL, whereas those of the chelating abilities on ferrous ions were 3.1 +/- 0.0, 4.6 +/- 0.1 and 4.9 +/- 0.1 mg/mL The EC(50) values of the antioxidant activity, of the DPPH scavenging, and of the reducing power were correlated with total polysaccharide as well as with total phenol content. The antioxidant activities of all the extracts may be caused by both polysaccharides and polyphenols or by a complex of both.",
publisher = "Elsevier",
journal = "Lwt-Food Science and Technology",
title = "Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune",
volume = "44",
number = "10",
pages = "2005-2011",
doi = "10.1016/j.lwt.2011.05.010"
}

Klaus, A., Kozarski, M. S., Nikšić, M., Jakovljević, D., Todorović, N.,& Van Griensven, L. J. L. D.. (2011). Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune. in Lwt-Food Science and Technology
Elsevier., 44(10), 2005-2011.
https://doi.org/10.1016/j.lwt.2011.05.010

Klaus A, Kozarski MS, Nikšić M, Jakovljević D, Todorović N, Van Griensven LJLD. Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune. in Lwt-Food Science and Technology. 2011;44(10):2005-2011.
doi:10.1016/j.lwt.2011.05.010 .

Klaus, Anita, Kozarski, Maja S., Nikšić, Miomir, Jakovljević, Dragica, Todorović, Nina, Van Griensven, Leo J. L. D., "Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune" in Lwt-Food Science and Technology, 44, no. 10 (2011):2005-2011,
https://doi.org/10.1016/j.lwt.2011.05.010 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Helsper, Johannes P. F. G.
AU  - Van Griensven, Leo J. L. D.
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/856
AB  - Partially purified polysaccharides were obtained from four medicinal mushroom species, Agaricus bisporus, Agaricus brasiliensis, Phellinus linteus and Ganoderma lucidum by hot water extraction, followed by ethanol precipitation. The four samples contained varying amounts of both alpha- and beta-glucans as determined by FT-IR and by quantitative estimation after prior partial hydrolysis (Megazyme beta-glucan assay kit). EC50 values of the DPPH scavenging activity of the polysaccharides from G. lucidum spores and P. linteus fruiting bodies were found to be particularly low, i.e. EC50  LT  0.1 mg/ml. For A. brasiliensis and A. bisporus, EC50 values were 0.27 and 2.0 mg/ml. EC50 values of the antioxidant activity were 7.07 mg/ml for G. lucidum, 13.25 mg/ml for A. brasiliensis and >20 mg/ml for A. bisporus polysaccharide, respectively. EC50 values of the chelating activity of ferrous ions ranged from 0.59 mg/ml for G. lucidum to 7.80 mg/ml for A. bisporus. The EC50 values of the extracts in the reducing power assay ranged from 0.47 to 14.83 mg/ml. A correlation was found between EC50 values of the chelating and reducing power abilities and the amount of total glucans content in the extracts. In vitro measurements of immunomodulatory capacity of polysaccharide extracts showed that A. bisporus, A. brasiliensis fruiting bodies and G. lucidum spores extracts express an immunostimulating effect on activated human PBMCs and induce synthesis of IFN-gamma. The polysaccharide extract of P. linteus fruiting bodies showed an immunosuppressive effect.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus
VL  - 129
IS  - 4
SP  - 1667
EP  - 1675
DO  - 10.1016/j.foodchem.2011.06.029
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Nikšić, Miomir and Jakovljević, Dragica and Helsper, Johannes P. F. G. and Van Griensven, Leo J. L. D.",
year = "2011",
abstract = "Partially purified polysaccharides were obtained from four medicinal mushroom species, Agaricus bisporus, Agaricus brasiliensis, Phellinus linteus and Ganoderma lucidum by hot water extraction, followed by ethanol precipitation. The four samples contained varying amounts of both alpha- and beta-glucans as determined by FT-IR and by quantitative estimation after prior partial hydrolysis (Megazyme beta-glucan assay kit). EC50 values of the DPPH scavenging activity of the polysaccharides from G. lucidum spores and P. linteus fruiting bodies were found to be particularly low, i.e. EC50  LT  0.1 mg/ml. For A. brasiliensis and A. bisporus, EC50 values were 0.27 and 2.0 mg/ml. EC50 values of the antioxidant activity were 7.07 mg/ml for G. lucidum, 13.25 mg/ml for A. brasiliensis and >20 mg/ml for A. bisporus polysaccharide, respectively. EC50 values of the chelating activity of ferrous ions ranged from 0.59 mg/ml for G. lucidum to 7.80 mg/ml for A. bisporus. The EC50 values of the extracts in the reducing power assay ranged from 0.47 to 14.83 mg/ml. A correlation was found between EC50 values of the chelating and reducing power abilities and the amount of total glucans content in the extracts. In vitro measurements of immunomodulatory capacity of polysaccharide extracts showed that A. bisporus, A. brasiliensis fruiting bodies and G. lucidum spores extracts express an immunostimulating effect on activated human PBMCs and induce synthesis of IFN-gamma. The polysaccharide extract of P. linteus fruiting bodies showed an immunosuppressive effect.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus",
volume = "129",
number = "4",
pages = "1667-1675",
doi = "10.1016/j.foodchem.2011.06.029"
}

Kozarski, M. S., Klaus, A., Nikšić, M., Jakovljević, D., Helsper, J. P. F. G.,& Van Griensven, L. J. L. D.. (2011). Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus. in Food Chemistry
Elsevier Sci Ltd, Oxford., 129(4), 1667-1675.
https://doi.org/10.1016/j.foodchem.2011.06.029

Kozarski MS, Klaus A, Nikšić M, Jakovljević D, Helsper JPFG, Van Griensven LJLD. Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus. in Food Chemistry. 2011;129(4):1667-1675.
doi:10.1016/j.foodchem.2011.06.029 .

Kozarski, Maja S., Klaus, Anita, Nikšić, Miomir, Jakovljević, Dragica, Helsper, Johannes P. F. G., Van Griensven, Leo J. L. D., "Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus" in Food Chemistry, 129, no. 4 (2011):1667-1675,
https://doi.org/10.1016/j.foodchem.2011.06.029 . .

TY  - JOUR
AU  - Vukosavljević, Predrag
AU  - Novaković, Miroslav
AU  - Bukvić, Branka
AU  - Nikšić, Miomir
AU  - Stanisavljević, Ivana
AU  - Klaus, Anita
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/548
AB  - This paper presents kinds of extraction and cross-flow filtration of composition of 46 healthful and aromatic herbs, 8 fruits and fungi Ganoderma lucidum. Those extracts are part of Bitter 55, which have significant antioxidant capacity. Antioxidative activities of plant extracts have been determined by DPPH test using method of Blois. Bitter 55 which was kept at the green bottle in the dark has EC50 = 141.07 μl/ml and it was stable during 150 days. Synthetic anti-oxidants BHT (ditertbutilhydroxytoluen, EC50 = 6.2 μgml-1), trolox (vitamin E analog soluble in water, EC50 = 6.8 μgml-1) were used for comparison. EC50 values were calculated as concentration of the extract necessary to decrease DPPH radical concentration for 50 %. Bitter 55 contents 35% vol of alcohol (wheat origin), 88.22 g/l total extract and slice of medicinal mushroom Ganoderma lucidum (1 % w/v) which was extracted 30 days before analyzes. The main problem in practical applications of MF is the reduction of permeate flux with time, caused by the accumulation of feed components in the pores. During microfiltration bitter herbal liquor, the function of filtrate flux is decreased with VCR. Dependence of decreasing flux with VCR can be separated in three periods. For the first, starting period, rapid decrease of filtrate flux is characteristic. Second period is defined with much smaller decrease of the flux than in the first phase. Third period has as characteristic minor decrease of flux and can be defined as steady state. Steady state emerges after τs = 80 min.
AB  - Sastojci lekovitog bilja i voća koji pokazuju potencijalnu antioksidativnu sposobnost su različite bioaktivne komponente, kao što su različiti fenoli, polifenoli, fenolne kiseline, flavonoidi, tanini i dr. Na tržištu se nalaze biljni ekstrakti od 12, 25 pa sve do 56 lekovitih i aromatičnih biljaka i voća. Dodatak medicinske gljive Ganoderma lucidum, biljnom ekstraktu od 54 lekovitih i aromatičnih biljaka, predstavlja dobro rešenje. Ekstrakcijom gljive Ganoderma (Ganoderma lucidum) zajedno sa lekovitim i aromatičnim biljem, zadržavaju se sve aktivne vrednosti biljaka, uz postizanje dodatnih lekovitih svojstava gljive. Ovakav proizvod, na bazi ekstrakata lekovitog i aromatičnog bilja, voća i gljive Ganoderma, je interesantan sa aspekta mogućih lekovitih svojstava. On može biti deklarisan ili kao gorki biljni liker u komercijalnoj prodaji ili čak kao dijetetski suplement za prodaju u apotekama. Takav proizvod u svakom slučaju predstavlja, kako zdravstveno tako i nutritivno, bolje rešenje od drugih alkoholnih pića. U radu su prikazani načini ekstrakcije i mikrofiltracije 46 lekovitih i aromatičnih biljaka, gljive Ganoderma lucidum i 8 voćnih vrsta koji čine kompoziciju Bittera 55. Utvrdjeno je da gorki biljni liker - Bitter 55 sadrži značajan antioksidativni potencijal. Potencijalna antioksidativna aktivnost određivana je DPPH testom, metodom po Blois-u.1 Vrednost EC50 uzorka BITTER-a 55 koji je stajao u zatamnjenoj boci u mraku je 139,47 μl/ml rastvarača i nije se promenila tokom 150 dana čuvanja. Sintetički antioksidansi BHT (ditercbutil-hidroksitoluen) i troloks (analog vitamina E rastvoran u vodi) imaju vrednosti EC50 redom 6,2 i 6,8 μg/ml. Vrednost EC50 predstavlja koncentraciju ekstrakta koja za 50% smanjuje koncentraciju DPPH radikala (praćeno preko inhibicije apsorpcije rastvora DPPH na 517nm).
PB  - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences
T1  - Antioxidant activities of herbs, fruit and medicinal mushroom Ganoderma lucidum extracts produced by microfiltration process
T1  - Mikrofiltracija i antioksidativni kapacitet kompozicije ekstrakta lekovitih biljaka, voća i medicinske gljive Ganoderma lucidum
VL  - 54
IS  - 1
SP  - 45
EP  - 62
DO  - 10.2298/JAS0901045V
ER  - 

@article{
author = "Vukosavljević, Predrag and Novaković, Miroslav and Bukvić, Branka and Nikšić, Miomir and Stanisavljević, Ivana and Klaus, Anita",
year = "2009",
abstract = "This paper presents kinds of extraction and cross-flow filtration of composition of 46 healthful and aromatic herbs, 8 fruits and fungi Ganoderma lucidum. Those extracts are part of Bitter 55, which have significant antioxidant capacity. Antioxidative activities of plant extracts have been determined by DPPH test using method of Blois. Bitter 55 which was kept at the green bottle in the dark has EC50 = 141.07 μl/ml and it was stable during 150 days. Synthetic anti-oxidants BHT (ditertbutilhydroxytoluen, EC50 = 6.2 μgml-1), trolox (vitamin E analog soluble in water, EC50 = 6.8 μgml-1) were used for comparison. EC50 values were calculated as concentration of the extract necessary to decrease DPPH radical concentration for 50 %. Bitter 55 contents 35% vol of alcohol (wheat origin), 88.22 g/l total extract and slice of medicinal mushroom Ganoderma lucidum (1 % w/v) which was extracted 30 days before analyzes. The main problem in practical applications of MF is the reduction of permeate flux with time, caused by the accumulation of feed components in the pores. During microfiltration bitter herbal liquor, the function of filtrate flux is decreased with VCR. Dependence of decreasing flux with VCR can be separated in three periods. For the first, starting period, rapid decrease of filtrate flux is characteristic. Second period is defined with much smaller decrease of the flux than in the first phase. Third period has as characteristic minor decrease of flux and can be defined as steady state. Steady state emerges after τs = 80 min., Sastojci lekovitog bilja i voća koji pokazuju potencijalnu antioksidativnu sposobnost su različite bioaktivne komponente, kao što su različiti fenoli, polifenoli, fenolne kiseline, flavonoidi, tanini i dr. Na tržištu se nalaze biljni ekstrakti od 12, 25 pa sve do 56 lekovitih i aromatičnih biljaka i voća. Dodatak medicinske gljive Ganoderma lucidum, biljnom ekstraktu od 54 lekovitih i aromatičnih biljaka, predstavlja dobro rešenje. Ekstrakcijom gljive Ganoderma (Ganoderma lucidum) zajedno sa lekovitim i aromatičnim biljem, zadržavaju se sve aktivne vrednosti biljaka, uz postizanje dodatnih lekovitih svojstava gljive. Ovakav proizvod, na bazi ekstrakata lekovitog i aromatičnog bilja, voća i gljive Ganoderma, je interesantan sa aspekta mogućih lekovitih svojstava. On može biti deklarisan ili kao gorki biljni liker u komercijalnoj prodaji ili čak kao dijetetski suplement za prodaju u apotekama. Takav proizvod u svakom slučaju predstavlja, kako zdravstveno tako i nutritivno, bolje rešenje od drugih alkoholnih pića. U radu su prikazani načini ekstrakcije i mikrofiltracije 46 lekovitih i aromatičnih biljaka, gljive Ganoderma lucidum i 8 voćnih vrsta koji čine kompoziciju Bittera 55. Utvrdjeno je da gorki biljni liker - Bitter 55 sadrži značajan antioksidativni potencijal. Potencijalna antioksidativna aktivnost određivana je DPPH testom, metodom po Blois-u.1 Vrednost EC50 uzorka BITTER-a 55 koji je stajao u zatamnjenoj boci u mraku je 139,47 μl/ml rastvarača i nije se promenila tokom 150 dana čuvanja. Sintetički antioksidansi BHT (ditercbutil-hidroksitoluen) i troloks (analog vitamina E rastvoran u vodi) imaju vrednosti EC50 redom 6,2 i 6,8 μg/ml. Vrednost EC50 predstavlja koncentraciju ekstrakta koja za 50% smanjuje koncentraciju DPPH radikala (praćeno preko inhibicije apsorpcije rastvora DPPH na 517nm).",
publisher = "Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences",
title = "Antioxidant activities of herbs, fruit and medicinal mushroom Ganoderma lucidum extracts produced by microfiltration process, Mikrofiltracija i antioksidativni kapacitet kompozicije ekstrakta lekovitih biljaka, voća i medicinske gljive Ganoderma lucidum",
volume = "54",
number = "1",
pages = "45-62",
doi = "10.2298/JAS0901045V"
}

Vukosavljević, P., Novaković, M., Bukvić, B., Nikšić, M., Stanisavljević, I.,& Klaus, A.. (2009). Antioxidant activities of herbs, fruit and medicinal mushroom Ganoderma lucidum extracts produced by microfiltration process. in Journal of Agricultural Sciences
Poljoprivredni fakultet, Beograd., 54(1), 45-62.
https://doi.org/10.2298/JAS0901045V

Vukosavljević P, Novaković M, Bukvić B, Nikšić M, Stanisavljević I, Klaus A. Antioxidant activities of herbs, fruit and medicinal mushroom Ganoderma lucidum extracts produced by microfiltration process. in Journal of Agricultural Sciences. 2009;54(1):45-62.
doi:10.2298/JAS0901045V .

Vukosavljević, Predrag, Novaković, Miroslav, Bukvić, Branka, Nikšić, Miomir, Stanisavljević, Ivana, Klaus, Anita, "Antioxidant activities of herbs, fruit and medicinal mushroom Ganoderma lucidum extracts produced by microfiltration process" in Journal of Agricultural Sciences, 54, no. 1 (2009):45-62,
https://doi.org/10.2298/JAS0901045V . .

TY  - JOUR
AU  - Vukosavljević, Predrag
AU  - Novaković, Miroslav
AU  - Bukvić, Branka
AU  - Nikšić, Miomir
AU  - Stanisavljević, Ivana
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/493
AB  - Bitter herbal liquors are products often consumed, not only because of their refreshment properties, but also healthy reasons. The main components of bitters are different herbal extracts which contain a lot of different bioactive compounds, such as phenols, polyphenols and their polymers, phenol carboxylic acids, organic acids flavonoids, tannins etc. At the market different products labeled as bitter herbal liquors can be found. They are made from 28, 32 or even 54 and 56 extracts obtained by alcohol or water extraction from different parts of herbs, fruits, seeds, spices, roots, etc. By definition 'bitters' are natural products made of fruits and herbs extracts permitted for human nutrition. Those products are not only alcohol beverages, but also the drinks with potentially healthful effects. Consumed in moderate ways they may provide intake of useful bioactive compounds. The paper presents extraction procedures of 46 different healthful and aromatic herbs and 8 fruits, used for production of 'Bitter 54'. Anti-oxidative activity of plant extracts was determinate by DPPH test using method of Blois. The free radical-scavenging activity of the plant extracts was evaluated using the stable radical DPPH. Synthetic antioxidants BHT (ditertbytil-hydroxytoluene, EC50= 6.2μgml-1), trolox (vitamine E analog soluble in water, EC50= 6.8μgml-1) were used for comparison. EC50 values were calculated as concentration of the extract necessary to decrease DPPH radical concentration for 50%. It was found that the EC50 value of Bitter kept in the green bottle in the dark has been 139.47μl/ml of solvent and it has been stable during 120 days. The results indicate that 'Bitter 54' possess significant antioxidant capacity. .
AB  - U radu su prikazani načini ekstrakcije 46 lekovitih i aromatičnih bilja i osam voćnih vrsta koji ulaze u sastav Bittera 54. Utvrđeno je da gorki biljni liker - Bitter 54 sadrži značajan antioksidativni potencijal. Potencijalna antioksidativna aktivnost određivana je testom DPPH, metodom po Bloisu. Vrednost EC50 uzorka Bittera 54 koji je stajao u zatamnjenoj boci u mraku bila je 139,47 μl/ml rastvarača i nije se promenila tokom 150 dana čuvanja. Sintetički antioksidansi BHT (ditercbutil-hidroksitoluen) i troloks (analog vitamina E rastvoran u vodi) imaju vrednosti EC50 6,2 i 6,8 μg/ml, respektivno. Vrednost EC50 predstavlja koncentraciju ekstrakta koja za 50% smanjuje koncentraciju radikala DPPH. .
PB  - Društvo za ishranu Srbije, Beograd
T2  - Hrana i ishrana
T1  - Anti-oxidant activities of herbs and fruits extracts in 'Bitter 54'
T1  - Potencijalni antioksidativni kapacitet kompozicije lekovitih i aromatičnih biljaka i voća u Bitteru 54
VL  - 50
IS  - 1-2
SP  - 3
EP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_cer_493
ER  - 

@article{
author = "Vukosavljević, Predrag and Novaković, Miroslav and Bukvić, Branka and Nikšić, Miomir and Stanisavljević, Ivana",
year = "2009",
abstract = "Bitter herbal liquors are products often consumed, not only because of their refreshment properties, but also healthy reasons. The main components of bitters are different herbal extracts which contain a lot of different bioactive compounds, such as phenols, polyphenols and their polymers, phenol carboxylic acids, organic acids flavonoids, tannins etc. At the market different products labeled as bitter herbal liquors can be found. They are made from 28, 32 or even 54 and 56 extracts obtained by alcohol or water extraction from different parts of herbs, fruits, seeds, spices, roots, etc. By definition 'bitters' are natural products made of fruits and herbs extracts permitted for human nutrition. Those products are not only alcohol beverages, but also the drinks with potentially healthful effects. Consumed in moderate ways they may provide intake of useful bioactive compounds. The paper presents extraction procedures of 46 different healthful and aromatic herbs and 8 fruits, used for production of 'Bitter 54'. Anti-oxidative activity of plant extracts was determinate by DPPH test using method of Blois. The free radical-scavenging activity of the plant extracts was evaluated using the stable radical DPPH. Synthetic antioxidants BHT (ditertbytil-hydroxytoluene, EC50= 6.2μgml-1), trolox (vitamine E analog soluble in water, EC50= 6.8μgml-1) were used for comparison. EC50 values were calculated as concentration of the extract necessary to decrease DPPH radical concentration for 50%. It was found that the EC50 value of Bitter kept in the green bottle in the dark has been 139.47μl/ml of solvent and it has been stable during 120 days. The results indicate that 'Bitter 54' possess significant antioxidant capacity. ., U radu su prikazani načini ekstrakcije 46 lekovitih i aromatičnih bilja i osam voćnih vrsta koji ulaze u sastav Bittera 54. Utvrđeno je da gorki biljni liker - Bitter 54 sadrži značajan antioksidativni potencijal. Potencijalna antioksidativna aktivnost određivana je testom DPPH, metodom po Bloisu. Vrednost EC50 uzorka Bittera 54 koji je stajao u zatamnjenoj boci u mraku bila je 139,47 μl/ml rastvarača i nije se promenila tokom 150 dana čuvanja. Sintetički antioksidansi BHT (ditercbutil-hidroksitoluen) i troloks (analog vitamina E rastvoran u vodi) imaju vrednosti EC50 6,2 i 6,8 μg/ml, respektivno. Vrednost EC50 predstavlja koncentraciju ekstrakta koja za 50% smanjuje koncentraciju radikala DPPH. .",
publisher = "Društvo za ishranu Srbije, Beograd",
journal = "Hrana i ishrana",
title = "Anti-oxidant activities of herbs and fruits extracts in 'Bitter 54', Potencijalni antioksidativni kapacitet kompozicije lekovitih i aromatičnih biljaka i voća u Bitteru 54",
volume = "50",
number = "1-2",
pages = "3-8",
url = "https://hdl.handle.net/21.15107/rcub_cer_493"
}

Vukosavljević, P., Novaković, M., Bukvić, B., Nikšić, M.,& Stanisavljević, I.. (2009). Anti-oxidant activities of herbs and fruits extracts in 'Bitter 54'. in Hrana i ishrana
Društvo za ishranu Srbije, Beograd., 50(1-2), 3-8.
https://hdl.handle.net/21.15107/rcub_cer_493

Vukosavljević P, Novaković M, Bukvić B, Nikšić M, Stanisavljević I. Anti-oxidant activities of herbs and fruits extracts in 'Bitter 54'. in Hrana i ishrana. 2009;50(1-2):3-8.
https://hdl.handle.net/21.15107/rcub_cer_493 .

Vukosavljević, Predrag, Novaković, Miroslav, Bukvić, Branka, Nikšić, Miomir, Stanisavljević, Ivana, "Anti-oxidant activities of herbs and fruits extracts in 'Bitter 54'" in Hrana i ishrana, 50, no. 1-2 (2009):3-8,
https://hdl.handle.net/21.15107/rcub_cer_493 .