TY  - JOUR
AU  - Đukanović, Stefana
AU  - Ganić, Tea
AU  - Lončarević, Branka
AU  - Cvetković, Stefana
AU  - Nikolić, Biljana
AU  - Tenji, Dina
AU  - Randjelović, Danijela
AU  - Mitić-Ćulafić, Dragana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5590
AB  - Aims Because the Staphylococcus aureus is one of the most well‐known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it.  Methods and Results Antibacterial activity was examined through microdilution assay. Antibiofilm testing included crystal violet staining of biofilm biomass and morphology analysis by Atomic force microscopy (AFM). Furthermore, aerobic respiration was monitored using the Micro‐Oxymax respirometer. For investigation of gene expression qRT‐PCR was performed. Emodin demonstrated strong antibacterial activity and ability to inhibit biofilm formation of all tested strains. The effect on preformed biofilms was spotted in few strains. AFM revealed that emodin affects biofilm structure and roughness. Monitoring of respiration under emodin treatment in planktonic and biofilm form revealed that emodin influenced aerobic respiration. Moreover, qRT‐PCR showed that emodin modulates expression of icaA, icaD, srrA and srrB genes, as well as RNAIII, and that this activity was strain‐specific.  Conclusion The results obtained in this study indicate the novel antibiofilm activity of emodin and its multiple pathways of action.  Significance and Impact of Study This is the first study that examined pathways through which emodin expressed its antibiofilm activity
PB  - Wiley
T2  - Journal of Applied Microbiology
T1  - Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms
VL  - 132
IS  - 3
SP  - 1840
EP  - 1855
DO  - 10.1111/jam.15360
ER  - 

@article{
author = "Đukanović, Stefana and Ganić, Tea and Lončarević, Branka and Cvetković, Stefana and Nikolić, Biljana and Tenji, Dina and Randjelović, Danijela and Mitić-Ćulafić, Dragana",
year = "2022",
abstract = "Aims Because the Staphylococcus aureus is one of the most well‐known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it.  Methods and Results Antibacterial activity was examined through microdilution assay. Antibiofilm testing included crystal violet staining of biofilm biomass and morphology analysis by Atomic force microscopy (AFM). Furthermore, aerobic respiration was monitored using the Micro‐Oxymax respirometer. For investigation of gene expression qRT‐PCR was performed. Emodin demonstrated strong antibacterial activity and ability to inhibit biofilm formation of all tested strains. The effect on preformed biofilms was spotted in few strains. AFM revealed that emodin affects biofilm structure and roughness. Monitoring of respiration under emodin treatment in planktonic and biofilm form revealed that emodin influenced aerobic respiration. Moreover, qRT‐PCR showed that emodin modulates expression of icaA, icaD, srrA and srrB genes, as well as RNAIII, and that this activity was strain‐specific.  Conclusion The results obtained in this study indicate the novel antibiofilm activity of emodin and its multiple pathways of action.  Significance and Impact of Study This is the first study that examined pathways through which emodin expressed its antibiofilm activity",
publisher = "Wiley",
journal = "Journal of Applied Microbiology",
title = "Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms",
volume = "132",
number = "3",
pages = "1840-1855",
doi = "10.1111/jam.15360"
}

Đukanović, S., Ganić, T., Lončarević, B., Cvetković, S., Nikolić, B., Tenji, D., Randjelović, D.,& Mitić-Ćulafić, D.. (2022). Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms. in Journal of Applied Microbiology
Wiley., 132(3), 1840-1855.
https://doi.org/10.1111/jam.15360

Đukanović S, Ganić T, Lončarević B, Cvetković S, Nikolić B, Tenji D, Randjelović D, Mitić-Ćulafić D. Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms. in Journal of Applied Microbiology. 2022;132(3):1840-1855.
doi:10.1111/jam.15360 .

Đukanović, Stefana, Ganić, Tea, Lončarević, Branka, Cvetković, Stefana, Nikolić, Biljana, Tenji, Dina, Randjelović, Danijela, Mitić-Ćulafić, Dragana, "Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms" in Journal of Applied Microbiology, 132, no. 3 (2022):1840-1855,
https://doi.org/10.1111/jam.15360 . .

TY  - CONF
AU  - Đukanović, Stefana
AU  - Lješević, Marija
AU  - Simin, Nataša
AU  - Lončarević, Branka
AU  - Ganić, Tea
AU  - Nikolić, Biljana
AU  - Cvetković, Stefana
AU  - Mitić-Ćulafić, Dragana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5148
AB  - The aim of the study was to chemically characterize the ethyl-acetate extract of F. alnus bark and investigate its antioxidant and antibacterial potential. Qualitative analysis of extract was done by GCxGC-MS while selected phenolics were quantified by LC-MS/MS analysis.
PB  - Opole, Poland : University of Opole
C3  - QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland
T1  - Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract
SP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_cer_5148
ER  - 

@conference{
author = "Đukanović, Stefana and Lješević, Marija and Simin, Nataša and Lončarević, Branka and Ganić, Tea and Nikolić, Biljana and Cvetković, Stefana and Mitić-Ćulafić, Dragana",
year = "2021",
abstract = "The aim of the study was to chemically characterize the ethyl-acetate extract of F. alnus bark and investigate its antioxidant and antibacterial potential. Qualitative analysis of extract was done by GCxGC-MS while selected phenolics were quantified by LC-MS/MS analysis.",
publisher = "Opole, Poland : University of Opole",
journal = "QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland",
title = "Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract",
pages = "147",
url = "https://hdl.handle.net/21.15107/rcub_cer_5148"
}

Đukanović, S., Lješević, M., Simin, N., Lončarević, B., Ganić, T., Nikolić, B., Cvetković, S.,& Mitić-Ćulafić, D.. (2021). Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract. in QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland
Opole, Poland : University of Opole., 147.
https://hdl.handle.net/21.15107/rcub_cer_5148

Đukanović S, Lješević M, Simin N, Lončarević B, Ganić T, Nikolić B, Cvetković S, Mitić-Ćulafić D. Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract. in QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland. 2021;:147.
https://hdl.handle.net/21.15107/rcub_cer_5148 .

Đukanović, Stefana, Lješević, Marija, Simin, Nataša, Lončarević, Branka, Ganić, Tea, Nikolić, Biljana, Cvetković, Stefana, Mitić-Ćulafić, Dragana, "Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract" in QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland (2021):147,
https://hdl.handle.net/21.15107/rcub_cer_5148 .

TY  - JOUR
AU  - Đukanović, Stefana
AU  - Cvetković, Stefana
AU  - Lončarević, Branka
AU  - Lješević, Marija
AU  - Nikolić, Biljana
AU  - Simin, Nataša
AU  - Bekvalac, Kristina
AU  - Kekić, Dušan
AU  - Mitić-Ćulafić, Dragana
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3946
AB  - Staphylococcus aureus is one of the leading causative of numerous infections that is resistant to various antibiotics. Since that we are facing with a serious problem of bacterial resistance it is necessary to find a new antibacterial agents in fight with it. Frangula alnus is traditionally used plant but its antibiofilm potential is poorly investigated. The aim of the study was to investigate antibiofilm activity of F. alnus ethyl-acetate extract (FA) toward S. aureus ATCC strains and clinical isolates as well as its influence on respiration in planktonic and biofilm form. The qualitative GCxGC–MS and quantitative LC–MS/MS analysis revealed that FA extract was rich in phenols and flavonoids and emodin, chatechin, and ester 4-ethoxy benzoic acid were the most dominant components. Results obtained through microdillution assay showed that FA possesses strong antibacterial activity. Furthermore, crystal violet staining of biofilm biomass demonstrated that extract had strong effect on biofilm formation of all tested strains while effect on preformed biofilms was less pronounced. The effect on biofilm was confirmed with scanning electron microscopy where the changes in biofilm structure were noticed. The activity of extract on the consumption of O2 and production of CO2 was monitored using the Micro-Oxymax respirometer. Interestingly, respiration of the most strains was decreased in planktonic form as well as in biofilms. Results obtained in this study are a good basis for further research in order to discover the mechanism of action of the FA extract on connection between biofilm and respiration.
PB  - Elsevier
T2  - Industrial Crops & Products
T1  - Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract
VL  - 158
SP  - 113013
DO  - 10.1016/j.indcrop.2020.113013
ER  - 

@article{
author = "Đukanović, Stefana and Cvetković, Stefana and Lončarević, Branka and Lješević, Marija and Nikolić, Biljana and Simin, Nataša and Bekvalac, Kristina and Kekić, Dušan and Mitić-Ćulafić, Dragana",
year = "2020",
abstract = "Staphylococcus aureus is one of the leading causative of numerous infections that is resistant to various antibiotics. Since that we are facing with a serious problem of bacterial resistance it is necessary to find a new antibacterial agents in fight with it. Frangula alnus is traditionally used plant but its antibiofilm potential is poorly investigated. The aim of the study was to investigate antibiofilm activity of F. alnus ethyl-acetate extract (FA) toward S. aureus ATCC strains and clinical isolates as well as its influence on respiration in planktonic and biofilm form. The qualitative GCxGC–MS and quantitative LC–MS/MS analysis revealed that FA extract was rich in phenols and flavonoids and emodin, chatechin, and ester 4-ethoxy benzoic acid were the most dominant components. Results obtained through microdillution assay showed that FA possesses strong antibacterial activity. Furthermore, crystal violet staining of biofilm biomass demonstrated that extract had strong effect on biofilm formation of all tested strains while effect on preformed biofilms was less pronounced. The effect on biofilm was confirmed with scanning electron microscopy where the changes in biofilm structure were noticed. The activity of extract on the consumption of O2 and production of CO2 was monitored using the Micro-Oxymax respirometer. Interestingly, respiration of the most strains was decreased in planktonic form as well as in biofilms. Results obtained in this study are a good basis for further research in order to discover the mechanism of action of the FA extract on connection between biofilm and respiration.",
publisher = "Elsevier",
journal = "Industrial Crops & Products",
title = "Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract",
volume = "158",
pages = "113013",
doi = "10.1016/j.indcrop.2020.113013"
}

Đukanović, S., Cvetković, S., Lončarević, B., Lješević, M., Nikolić, B., Simin, N., Bekvalac, K., Kekić, D.,& Mitić-Ćulafić, D.. (2020). Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract. in Industrial Crops & Products
Elsevier., 158, 113013.
https://doi.org/10.1016/j.indcrop.2020.113013

Đukanović S, Cvetković S, Lončarević B, Lješević M, Nikolić B, Simin N, Bekvalac K, Kekić D, Mitić-Ćulafić D. Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract. in Industrial Crops & Products. 2020;158:113013.
doi:10.1016/j.indcrop.2020.113013 .

Đukanović, Stefana, Cvetković, Stefana, Lončarević, Branka, Lješević, Marija, Nikolić, Biljana, Simin, Nataša, Bekvalac, Kristina, Kekić, Dušan, Mitić-Ćulafić, Dragana, "Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract" in Industrial Crops & Products, 158 (2020):113013,
https://doi.org/10.1016/j.indcrop.2020.113013 . .

TY  - JOUR
AU  - Doroški, Ana
AU  - Klaus, Anita
AU  - Kozarski, Maja
AU  - Cvetković, Stefana
AU  - Nikolić, Biljana
AU  - Jakovljević, Dragica
AU  - Tomasević, Igor
AU  - Vunduk, Jovana
AU  - Lazić, Vesna
AU  - Đekić, Ilija
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4021
AB  - Total quality index approach was used to reflect the quality of Pleurotus ostreatus mushroom, cultivated on grape pomace (P) as food waste substrate and wheat straw (S), in four different combined ratios: 100P; 80P20S; 50P50S; and 20P80S, with 100S, serving as control substrate. This approach involved two directions of mushroom analysis. The whole mushroom fruiting bodies analysis introduced a single total quality index (TQIM) based on 11 quality parameters. The mushroom water extracts analysis was presented by another single total quality index (TQIE), with seven quality parameters. This mathematical model revealed that the mushroom extracts have a better overall quality score with higher pomace content in substrate mixture, compared to the whole mushroom quality changes throughout the observed shelf life period. Future research may pay attention to the phytotoxicity of grape pomace as a food waste, as well as the influence of grape pomace on glucan content, as a health‐beneficial compounds.
PB  - Wiley
T2  - Journal of Food Processing and Preservation
T1  - The influence of grape pomace substrate on quality characterization of                    Pleurotus ostreatus                    —Total quality index approach
SP  - e15096
DO  - 10.1111/jfpp.15096
ER  - 

@article{
author = "Doroški, Ana and Klaus, Anita and Kozarski, Maja and Cvetković, Stefana and Nikolić, Biljana and Jakovljević, Dragica and Tomasević, Igor and Vunduk, Jovana and Lazić, Vesna and Đekić, Ilija",
year = "2020",
abstract = "Total quality index approach was used to reflect the quality of Pleurotus ostreatus mushroom, cultivated on grape pomace (P) as food waste substrate and wheat straw (S), in four different combined ratios: 100P; 80P20S; 50P50S; and 20P80S, with 100S, serving as control substrate. This approach involved two directions of mushroom analysis. The whole mushroom fruiting bodies analysis introduced a single total quality index (TQIM) based on 11 quality parameters. The mushroom water extracts analysis was presented by another single total quality index (TQIE), with seven quality parameters. This mathematical model revealed that the mushroom extracts have a better overall quality score with higher pomace content in substrate mixture, compared to the whole mushroom quality changes throughout the observed shelf life period. Future research may pay attention to the phytotoxicity of grape pomace as a food waste, as well as the influence of grape pomace on glucan content, as a health‐beneficial compounds.",
publisher = "Wiley",
journal = "Journal of Food Processing and Preservation",
title = "The influence of grape pomace substrate on quality characterization of                    Pleurotus ostreatus                    —Total quality index approach",
pages = "e15096",
doi = "10.1111/jfpp.15096"
}

Doroški, A., Klaus, A., Kozarski, M., Cvetković, S., Nikolić, B., Jakovljević, D., Tomasević, I., Vunduk, J., Lazić, V.,& Đekić, I.. (2020). The influence of grape pomace substrate on quality characterization of                    Pleurotus ostreatus                    —Total quality index approach. in Journal of Food Processing and Preservation
Wiley., e15096.
https://doi.org/10.1111/jfpp.15096

Doroški A, Klaus A, Kozarski M, Cvetković S, Nikolić B, Jakovljević D, Tomasević I, Vunduk J, Lazić V, Đekić I. The influence of grape pomace substrate on quality characterization of                    Pleurotus ostreatus                    —Total quality index approach. in Journal of Food Processing and Preservation. 2020;:e15096.
doi:10.1111/jfpp.15096 .

Doroški, Ana, Klaus, Anita, Kozarski, Maja, Cvetković, Stefana, Nikolić, Biljana, Jakovljević, Dragica, Tomasević, Igor, Vunduk, Jovana, Lazić, Vesna, Đekić, Ilija, "The influence of grape pomace substrate on quality characterization of                    Pleurotus ostreatus                    —Total quality index approach" in Journal of Food Processing and Preservation (2020):e15096,
https://doi.org/10.1111/jfpp.15096 . .