TY  - CONF
AU  - Ognjanović, Miloš
AU  - Radović, Magdalena
AU  - Mirković, Marija
AU  - Vranješ-Đurić, Sanja
AU  - Dojčinović, Biljana
AU  - Stanković, Dalibor
AU  - Antić, Bratislav
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7342
AB  - In the last decades, self-heating magnetic nanoparticles (MNPs) were engineered and investigated for magnetic hyperthermia (MH) and other applications such as catalysis and chemical synthesis. To be applied as nanoheaters for in vivo MH in cancer therapy, MNPs should have high heating efficiency expressed by Intrinsic Loss Power (ILP). One of the requirements for in vivo applications of MNPs is their non-toxicity. Hence, the most investigated MNPs for MH are based on iron oxides (magnetite and maghemite), which are non-toxic or slightly toxic. This work aimed to apply thepolyol-mediated protocol to engineer mixed Zn1-xMnxFe2O4 and analyze their heating abilities. To obtain a series of Zn1-xMnxFe2O4 samples with a specific nominal composition, the initial components, salts of Zn, Mn and Fe, were mixed in the appropriate stoichiometric ratio. The deviation from the target stoichiometry and the formation of samples with polyvalent ions and possibly vacancies were determined after ICP analysis. By analyzing TEM micrographs, we found that the change in the chemical composition does not affect the morphology. Multicore flower-like nanostructures with a size in the range of 47-63 nm were obtained. They consist of many cores (crystallites or nanoparticles) with a size of \textasciitilde10 nm. The samples show good colloidal stability, which is significant for their medical applications. Magnetization measurements in different DC fields showed that the samples are superparamagnetic at 300K and that the saturation magnetization values are in the range of \textasciitilde59-73 emu/g. The hyperthermic efficiency of the synthesized samples was tested in an external ac field of 252 kHz and a field strength of 15.9 kA/m. Significantly different values were obtained for the ILP parameter (in units nHm2/Kg): 5.77 (Zn0.098Mn0.447Fe2.455O4) ˃ 3.22 (Mn0.624Fe2.376O4) ˃ 2.04 (Zn0.182Mn0.344Fe2.474O4) ˃ 1.36 (Zn0.309Mn0.240Fe2.451O4) ˃ 1.01 (Zn0.394Mn0.138Fe2.468O4) ˃ 0.34 (Zn0.640Fe2.360O4). To explain the values of the ILP parameter, additional research is required, which includes the analysis of the influence of local defects and cation distribution on the magnetism of the investigated nanostructures. Also, significantly high ILP values indicate that some samples can be selected and further tested for in vitro/in vivo applications.
PB  - Society of Chemists and Technologists of Macedonia
C3  - 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia
T1  - Engineering multi-core flower-like magnetic nanoparticles with high intrinsic loss power
SP  - 185
EP  - 185
UR  - https://hdl.handle.net/21.15107/rcub_cer_7342
ER  - 

@conference{
author = "Ognjanović, Miloš and Radović, Magdalena and Mirković, Marija and Vranješ-Đurić, Sanja and Dojčinović, Biljana and Stanković, Dalibor and Antić, Bratislav",
year = "2023",
abstract = "In the last decades, self-heating magnetic nanoparticles (MNPs) were engineered and investigated for magnetic hyperthermia (MH) and other applications such as catalysis and chemical synthesis. To be applied as nanoheaters for in vivo MH in cancer therapy, MNPs should have high heating efficiency expressed by Intrinsic Loss Power (ILP). One of the requirements for in vivo applications of MNPs is their non-toxicity. Hence, the most investigated MNPs for MH are based on iron oxides (magnetite and maghemite), which are non-toxic or slightly toxic. This work aimed to apply thepolyol-mediated protocol to engineer mixed Zn1-xMnxFe2O4 and analyze their heating abilities. To obtain a series of Zn1-xMnxFe2O4 samples with a specific nominal composition, the initial components, salts of Zn, Mn and Fe, were mixed in the appropriate stoichiometric ratio. The deviation from the target stoichiometry and the formation of samples with polyvalent ions and possibly vacancies were determined after ICP analysis. By analyzing TEM micrographs, we found that the change in the chemical composition does not affect the morphology. Multicore flower-like nanostructures with a size in the range of 47-63 nm were obtained. They consist of many cores (crystallites or nanoparticles) with a size of \textasciitilde10 nm. The samples show good colloidal stability, which is significant for their medical applications. Magnetization measurements in different DC fields showed that the samples are superparamagnetic at 300K and that the saturation magnetization values are in the range of \textasciitilde59-73 emu/g. The hyperthermic efficiency of the synthesized samples was tested in an external ac field of 252 kHz and a field strength of 15.9 kA/m. Significantly different values were obtained for the ILP parameter (in units nHm2/Kg): 5.77 (Zn0.098Mn0.447Fe2.455O4) ˃ 3.22 (Mn0.624Fe2.376O4) ˃ 2.04 (Zn0.182Mn0.344Fe2.474O4) ˃ 1.36 (Zn0.309Mn0.240Fe2.451O4) ˃ 1.01 (Zn0.394Mn0.138Fe2.468O4) ˃ 0.34 (Zn0.640Fe2.360O4). To explain the values of the ILP parameter, additional research is required, which includes the analysis of the influence of local defects and cation distribution on the magnetism of the investigated nanostructures. Also, significantly high ILP values indicate that some samples can be selected and further tested for in vitro/in vivo applications.",
publisher = "Society of Chemists and Technologists of Macedonia",
journal = "26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia",
title = "Engineering multi-core flower-like magnetic nanoparticles with high intrinsic loss power",
pages = "185-185",
url = "https://hdl.handle.net/21.15107/rcub_cer_7342"
}

Ognjanović, M., Radović, M., Mirković, M., Vranješ-Đurić, S., Dojčinović, B., Stanković, D.,& Antić, B.. (2023). Engineering multi-core flower-like magnetic nanoparticles with high intrinsic loss power. in 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia
Society of Chemists and Technologists of Macedonia., 185-185.
https://hdl.handle.net/21.15107/rcub_cer_7342

Ognjanović M, Radović M, Mirković M, Vranješ-Đurić S, Dojčinović B, Stanković D, Antić B. Engineering multi-core flower-like magnetic nanoparticles with high intrinsic loss power. in 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia. 2023;:185-185.
https://hdl.handle.net/21.15107/rcub_cer_7342 .

Ognjanović, Miloš, Radović, Magdalena, Mirković, Marija, Vranješ-Đurić, Sanja, Dojčinović, Biljana, Stanković, Dalibor, Antić, Bratislav, "Engineering multi-core flower-like magnetic nanoparticles with high intrinsic loss power" in 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia (2023):185-185,
https://hdl.handle.net/21.15107/rcub_cer_7342 .

TY  - CONF
AU  - Ognjanović, Miloš
AU  - Stanojković, Tatjana
AU  - Dojčinović, Biljana
AU  - Radović, Magdalena
AU  - Mirković, Marija
AU  - Vranješ-Đurić, Sanja
AU  - Antić, Bratislav
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7349
AB  - A series of MgxFe3-xO4 (x=0, 0.1, 0.2, 0.4, 0.6, 0.8, and 1) magnetic nanoparticles (MNP) were synthesized by a two-step procedure, a co-precipitation method followed by hydrothermal treatment in a microwave field. The MNP are single-core, with crystallite size gradually decreasing from 15.5(3) up to 2.5(3) nm with an increase ofx. TEM images show pseudospherical log-normally distributed particles with an average particle diameter of 19.8 nm and a polydispersity index of 26.1% for magnetite. The particle diameter decreases with the increase of magnesium (x) in the formula unit. The colloidal stability of MNP was achieved by their surface modification with citric acid (CA), oleic acid (OA) and polyethylene glycol (PEG). The cytotoxic activity of uncoated and coated Mg0.6Fe2.4O4 was tested against target malignant cells (HeLa, LC174, A549) and normal MRC5 cells. The investigated MNP show moderate cytotoxic activity against the tested malignant cells in vitro. In contrast, MNP didn’tshow any significant cytotoxic effect against normal cells. HeLa cells exhibited the highest susceptibility among the malignant cells. Mg0.6Fe2.4O4@OA show good cytotoxic activity against all examined malignant cells, significantly higher than other tested MNP. It can be seen that Mg0.6Fe2.4O4@PEG show a lower cytotoxic activity compared to all analyzed MNP. A direct method was used for labeling with radionuclide 90Y, which involves incubation of MNP with 90Y at a certain temperature and time. The labeling yield of the 90Y-coated MNP was determined by analyzing the radiochemical purity after labeling. 90YMg0.2Fe2.8O4@PEG were labeled in high yield (100%), while the yield for 90YMg0.2Fe2.8O4@CA was 83%. In vitro stability of 90Y-coated MNP at room temperature in physiological solution and human serum was monitored within 72 h from the moment of labeling by determining the radiochemical purity of ITLC-SG by radio chromatographic method. The stability of 90Y-Mg0.2Fe2.8O4@PEG was about 97%, while 90Y-Mg0.2Fe2.8O4@CA stability was 73%. The results of this study indicate that radiolabeled surface-modified (Mg, Fe)3O4 can be used as vectors in radionuclide therapy of malignant diseases.
PB  - Society of Chemists and Technologists of Macedonia
C3  - 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia
T1  - Radiolabeled surface-modified single-core (Mg,Fe)3O4 colloidal nanoparticles as vectors in radionuclidetherapy of cancer
SP  - 186
EP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_cer_7349
ER  - 

@conference{
author = "Ognjanović, Miloš and Stanojković, Tatjana and Dojčinović, Biljana and Radović, Magdalena and Mirković, Marija and Vranješ-Đurić, Sanja and Antić, Bratislav",
year = "2023",
abstract = "A series of MgxFe3-xO4 (x=0, 0.1, 0.2, 0.4, 0.6, 0.8, and 1) magnetic nanoparticles (MNP) were synthesized by a two-step procedure, a co-precipitation method followed by hydrothermal treatment in a microwave field. The MNP are single-core, with crystallite size gradually decreasing from 15.5(3) up to 2.5(3) nm with an increase ofx. TEM images show pseudospherical log-normally distributed particles with an average particle diameter of 19.8 nm and a polydispersity index of 26.1% for magnetite. The particle diameter decreases with the increase of magnesium (x) in the formula unit. The colloidal stability of MNP was achieved by their surface modification with citric acid (CA), oleic acid (OA) and polyethylene glycol (PEG). The cytotoxic activity of uncoated and coated Mg0.6Fe2.4O4 was tested against target malignant cells (HeLa, LC174, A549) and normal MRC5 cells. The investigated MNP show moderate cytotoxic activity against the tested malignant cells in vitro. In contrast, MNP didn’tshow any significant cytotoxic effect against normal cells. HeLa cells exhibited the highest susceptibility among the malignant cells. Mg0.6Fe2.4O4@OA show good cytotoxic activity against all examined malignant cells, significantly higher than other tested MNP. It can be seen that Mg0.6Fe2.4O4@PEG show a lower cytotoxic activity compared to all analyzed MNP. A direct method was used for labeling with radionuclide 90Y, which involves incubation of MNP with 90Y at a certain temperature and time. The labeling yield of the 90Y-coated MNP was determined by analyzing the radiochemical purity after labeling. 90YMg0.2Fe2.8O4@PEG were labeled in high yield (100%), while the yield for 90YMg0.2Fe2.8O4@CA was 83%. In vitro stability of 90Y-coated MNP at room temperature in physiological solution and human serum was monitored within 72 h from the moment of labeling by determining the radiochemical purity of ITLC-SG by radio chromatographic method. The stability of 90Y-Mg0.2Fe2.8O4@PEG was about 97%, while 90Y-Mg0.2Fe2.8O4@CA stability was 73%. The results of this study indicate that radiolabeled surface-modified (Mg, Fe)3O4 can be used as vectors in radionuclide therapy of malignant diseases.",
publisher = "Society of Chemists and Technologists of Macedonia",
journal = "26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia",
title = "Radiolabeled surface-modified single-core (Mg,Fe)3O4 colloidal nanoparticles as vectors in radionuclidetherapy of cancer",
pages = "186-186",
url = "https://hdl.handle.net/21.15107/rcub_cer_7349"
}

Ognjanović, M., Stanojković, T., Dojčinović, B., Radović, M., Mirković, M., Vranješ-Đurić, S.,& Antić, B.. (2023). Radiolabeled surface-modified single-core (Mg,Fe)3O4 colloidal nanoparticles as vectors in radionuclidetherapy of cancer. in 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia
Society of Chemists and Technologists of Macedonia., 186-186.
https://hdl.handle.net/21.15107/rcub_cer_7349

Ognjanović M, Stanojković T, Dojčinović B, Radović M, Mirković M, Vranješ-Đurić S, Antić B. Radiolabeled surface-modified single-core (Mg,Fe)3O4 colloidal nanoparticles as vectors in radionuclidetherapy of cancer. in 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia. 2023;:186-186.
https://hdl.handle.net/21.15107/rcub_cer_7349 .

Ognjanović, Miloš, Stanojković, Tatjana, Dojčinović, Biljana, Radović, Magdalena, Mirković, Marija, Vranješ-Đurić, Sanja, Antić, Bratislav, "Radiolabeled surface-modified single-core (Mg,Fe)3O4 colloidal nanoparticles as vectors in radionuclidetherapy of cancer" in 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia (2023):186-186,
https://hdl.handle.net/21.15107/rcub_cer_7349 .