TY  - JOUR
AU  - Savković, Željko
AU  - Stupar, Miloš
AU  - Unković, Nikola
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Popović, Slađana
AU  - Vukojević, Jelena
AU  - Ljaljević Grbić, Milica
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4051
AB  - An extensive aeromycological survey was performed in the premises of a cultural heritage conservation facility to estimate fungal propagule concentrations in both indoor and outdoor air and seasonal dynamics with regard to temperature and air humidity. The highest average propagule concentration in indoor air was documented in spring, and for outdoor air, in summer. Increased propagule loads were documented in most rooms during winter, fall and spring, and in all examined rooms in summer. Very high propagule concentrations (>25,000 CFU m−3) were reported in ground-floor rooms. The total mycobiota was comprised of 74 fungal species, with a prevalence of Aspergilli and Penicillia. Cladosporium and Penicillium isolates were found to be the most abundant in all seasons in both indoor and outdoor air samples. Among the identified fungi, potential human pathogens, allergens and mycotoxin producers were present. Numerous documented species play an important role in the deterioration of cultural heritage artifacts and are known producers of extracellular enzymes, acids and pigments. Application of a selective medium (M40Y) allowed isolation of various xerophiles and xerotolerant species. Aeromycological analyses are mandatory for determining the appropriate conditions for the protection of the health of conservators, as well as the objects of cultural heritage.
PB  - Elsevier
T2  - International Biodeterioration & Biodegradation
T1  - Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility
VL  - 157
SP  - 105163
DO  - 10.1016/j.ibiod.2020.105163
ER  - 

@article{
author = "Savković, Željko and Stupar, Miloš and Unković, Nikola and Ivanović, Žarko and Blagojević, Jovana and Popović, Slađana and Vukojević, Jelena and Ljaljević Grbić, Milica",
year = "2021",
abstract = "An extensive aeromycological survey was performed in the premises of a cultural heritage conservation facility to estimate fungal propagule concentrations in both indoor and outdoor air and seasonal dynamics with regard to temperature and air humidity. The highest average propagule concentration in indoor air was documented in spring, and for outdoor air, in summer. Increased propagule loads were documented in most rooms during winter, fall and spring, and in all examined rooms in summer. Very high propagule concentrations (>25,000 CFU m−3) were reported in ground-floor rooms. The total mycobiota was comprised of 74 fungal species, with a prevalence of Aspergilli and Penicillia. Cladosporium and Penicillium isolates were found to be the most abundant in all seasons in both indoor and outdoor air samples. Among the identified fungi, potential human pathogens, allergens and mycotoxin producers were present. Numerous documented species play an important role in the deterioration of cultural heritage artifacts and are known producers of extracellular enzymes, acids and pigments. Application of a selective medium (M40Y) allowed isolation of various xerophiles and xerotolerant species. Aeromycological analyses are mandatory for determining the appropriate conditions for the protection of the health of conservators, as well as the objects of cultural heritage.",
publisher = "Elsevier",
journal = "International Biodeterioration & Biodegradation",
title = "Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility",
volume = "157",
pages = "105163",
doi = "10.1016/j.ibiod.2020.105163"
}

Savković, Ž., Stupar, M., Unković, N., Ivanović, Ž., Blagojević, J., Popović, S., Vukojević, J.,& Ljaljević Grbić, M.. (2021). Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility. in International Biodeterioration & Biodegradation
Elsevier., 157, 105163.
https://doi.org/10.1016/j.ibiod.2020.105163

Savković Ž, Stupar M, Unković N, Ivanović Ž, Blagojević J, Popović S, Vukojević J, Ljaljević Grbić M. Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility. in International Biodeterioration & Biodegradation. 2021;157:105163.
doi:10.1016/j.ibiod.2020.105163 .

Savković, Željko, Stupar, Miloš, Unković, Nikola, Ivanović, Žarko, Blagojević, Jovana, Popović, Slađana, Vukojević, Jelena, Ljaljević Grbić, Milica, "Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility" in International Biodeterioration & Biodegradation, 157 (2021):105163,
https://doi.org/10.1016/j.ibiod.2020.105163 . .

TY  - CONF
AU  - Breka, Katarina
AU  - Stupar, Miloš
AU  - Vidaković, Danijela
AU  - Ljaljević Grbić, Milica
AU  - Krizmanić, Jelena
AU  - Krizmanić, Imre
AU  - Stamenković, Srđan
PY  - 2019
UR  - http://seh-congress-2019.unipv.it/wp-content/uploads/2019/08/SEHXX-abstract-book-3.pdf
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4621
AB  - Amphibian populations have been declining throughout the world in the last several decades. Although
numerous factors, including habitat destruction, pollution, introduced species, and global environmental changes
contribute the most to amphibian population decline, yet many amphibians are dying of infectious diseases.
Recent studies of amphibian skin infections were mainly focused on the presence of chytridiomycosis
neglecting other members of the frogs’ skin communities. Samples for mycological and algological analyses
were collected from 100 individuals belonging to Pelophylax esculenta complex on three localities in South
Banat, Serbia using non-aggressive, rapid and inexpensive “adhesive tape” method for microbial biofilm
observation.
In analysed samples variety of fungal reproductive structures, and in few samples spore germination,
and mycelia formation were detected. Sporulation of some typical soil-borne fungi and the causative agent of
amphibian chromomycosis as well as human phaeohyphomycosis, Fonseceae sp. was detected along with water
mold, Aphanomyces sp. the pathogen responsible for amphibian egg mortality and saprolegniasis of mostly
salamanders and tadpoles. For both pathogens, these were the first reports for green frogs in Serbia. Microscopy
analysis showed the presence of aeroaquatic hyphomycetes (eg. Canalisporium sp.). Besides mycobiota, the
frog's skin has proved to be a good medium for the survival of diatoms (detected in high diversity and
abundance), Cyanobacteria and Chlorophyta.
Adhesive tape method proved itself useful as a consequential diagnostic tool for preliminary
observation of the microbial community on the skin of amphibians including potential pathogens and symbionts.
It provides minimal stress to the studied animal and can be easily applicable in various conditions of field
research.
PB  - Societas Europaea herpetologica (SEH)
C3  - Program and abstracts - XX European Congress of Herpetology
T1  - Microbial community of green frog’s skin
SP  - 239
EP  - 239
UR  - https://hdl.handle.net/21.15107/rcub_cer_4621
ER  - 

@conference{
author = "Breka, Katarina and Stupar, Miloš and Vidaković, Danijela and Ljaljević Grbić, Milica and Krizmanić, Jelena and Krizmanić, Imre and Stamenković, Srđan",
year = "2019",
abstract = "Amphibian populations have been declining throughout the world in the last several decades. Although
numerous factors, including habitat destruction, pollution, introduced species, and global environmental changes
contribute the most to amphibian population decline, yet many amphibians are dying of infectious diseases.
Recent studies of amphibian skin infections were mainly focused on the presence of chytridiomycosis
neglecting other members of the frogs’ skin communities. Samples for mycological and algological analyses
were collected from 100 individuals belonging to Pelophylax esculenta complex on three localities in South
Banat, Serbia using non-aggressive, rapid and inexpensive “adhesive tape” method for microbial biofilm
observation.
In analysed samples variety of fungal reproductive structures, and in few samples spore germination,
and mycelia formation were detected. Sporulation of some typical soil-borne fungi and the causative agent of
amphibian chromomycosis as well as human phaeohyphomycosis, Fonseceae sp. was detected along with water
mold, Aphanomyces sp. the pathogen responsible for amphibian egg mortality and saprolegniasis of mostly
salamanders and tadpoles. For both pathogens, these were the first reports for green frogs in Serbia. Microscopy
analysis showed the presence of aeroaquatic hyphomycetes (eg. Canalisporium sp.). Besides mycobiota, the
frog's skin has proved to be a good medium for the survival of diatoms (detected in high diversity and
abundance), Cyanobacteria and Chlorophyta.
Adhesive tape method proved itself useful as a consequential diagnostic tool for preliminary
observation of the microbial community on the skin of amphibians including potential pathogens and symbionts.
It provides minimal stress to the studied animal and can be easily applicable in various conditions of field
research.",
publisher = "Societas Europaea herpetologica (SEH)",
journal = "Program and abstracts - XX European Congress of Herpetology",
title = "Microbial community of green frog’s skin",
pages = "239-239",
url = "https://hdl.handle.net/21.15107/rcub_cer_4621"
}

Breka, K., Stupar, M., Vidaković, D., Ljaljević Grbić, M., Krizmanić, J., Krizmanić, I.,& Stamenković, S.. (2019). Microbial community of green frog’s skin. in Program and abstracts - XX European Congress of Herpetology
Societas Europaea herpetologica (SEH)., 239-239.
https://hdl.handle.net/21.15107/rcub_cer_4621

Breka K, Stupar M, Vidaković D, Ljaljević Grbić M, Krizmanić J, Krizmanić I, Stamenković S. Microbial community of green frog’s skin. in Program and abstracts - XX European Congress of Herpetology. 2019;:239-239.
https://hdl.handle.net/21.15107/rcub_cer_4621 .

Breka, Katarina, Stupar, Miloš, Vidaković, Danijela, Ljaljević Grbić, Milica, Krizmanić, Jelena, Krizmanić, Imre, Stamenković, Srđan, "Microbial community of green frog’s skin" in Program and abstracts - XX European Congress of Herpetology (2019):239-239,
https://hdl.handle.net/21.15107/rcub_cer_4621 .

TY  - CONF
AU  - Krizmanić, Jelena
AU  - Stupar, Miloš
AU  - Ljaljević Grbić, Milica
AU  - Dimitrijević, Jelica
AU  - Vidaković, Danijela
AU  - Stamenković, Srđan
AU  - Krizmanić, Imre
AU  - Breka, Katarina
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4622
AB  - Cutaneous microbiota of amphibians is considered
to be an indicator of the health status of examined
individuals, as well as of environmental factors. In
that sense the skin of the captured individuals
belonging to Pelophylax esculenta complex on the
locality Stevanove ravnice within the Special Nature
Reserve “Deliblato Sand” (Serbia) was screened for
the presence of biofilm-forming cyanobacteria,
algae and fungi dwelling directly on frogs’ stratum
corneum. Biofilm was sampled using non-aggressive
“adhesive tape method” and their constituents were
analyzed and characterized microscopically (Carl
Zeiss AxioImager.M1 with AxioVision 4.9 software).
We detected high diversity and abundance
of diatom taxa overall, particularly belonging to
genera Navicula, Gomphonema, Geissleria,
Placoneis and Nitzschia. Cyanobacteria were less
present and with low abundance. Among them
chroococcal genera Gloeocapsopsis, Pseudocapsa
and Aphanocapsa were recorded. Outside of diatoms
and cyanobacteria, genus Chlorella was the
only present photoautotrophic biofilm constituent.
We consider highly mucous green frogs skin as
a favorable environment for the survival of diatoms
and cyanobacteria. Light microscopy analyses of
biofilm fragments revealed the presence of variety
of fungal structures. Documented coenocytic mycelium
with zoosporangium, originated presumably
from oomycetes, as well as septate melanized
hyphae of dematiaceous fungi, suggested colonization
of frog skin from both types of environments
present, water and surrounding vegetation.
A variety of fungal spores (fragmospores, amerospores,
dictyospores, didymospores), and in few
cases spore germination were recorded on frog
skin. Detected biofilm composition was not determinate
by species and gender-specific.
PB  - Taylor & Francis
C3  - European Journal of Phycology (Posters)
T1  - Green frogs skin harbours microbiota treasure
VL  - 54
IS  - sup1
SP  - 156
EP  - 156
DO  - 10.1080/09670262.2019.1626628
ER  - 

@conference{
author = "Krizmanić, Jelena and Stupar, Miloš and Ljaljević Grbić, Milica and Dimitrijević, Jelica and Vidaković, Danijela and Stamenković, Srđan and Krizmanić, Imre and Breka, Katarina",
year = "2019",
abstract = "Cutaneous microbiota of amphibians is considered
to be an indicator of the health status of examined
individuals, as well as of environmental factors. In
that sense the skin of the captured individuals
belonging to Pelophylax esculenta complex on the
locality Stevanove ravnice within the Special Nature
Reserve “Deliblato Sand” (Serbia) was screened for
the presence of biofilm-forming cyanobacteria,
algae and fungi dwelling directly on frogs’ stratum
corneum. Biofilm was sampled using non-aggressive
“adhesive tape method” and their constituents were
analyzed and characterized microscopically (Carl
Zeiss AxioImager.M1 with AxioVision 4.9 software).
We detected high diversity and abundance
of diatom taxa overall, particularly belonging to
genera Navicula, Gomphonema, Geissleria,
Placoneis and Nitzschia. Cyanobacteria were less
present and with low abundance. Among them
chroococcal genera Gloeocapsopsis, Pseudocapsa
and Aphanocapsa were recorded. Outside of diatoms
and cyanobacteria, genus Chlorella was the
only present photoautotrophic biofilm constituent.
We consider highly mucous green frogs skin as
a favorable environment for the survival of diatoms
and cyanobacteria. Light microscopy analyses of
biofilm fragments revealed the presence of variety
of fungal structures. Documented coenocytic mycelium
with zoosporangium, originated presumably
from oomycetes, as well as septate melanized
hyphae of dematiaceous fungi, suggested colonization
of frog skin from both types of environments
present, water and surrounding vegetation.
A variety of fungal spores (fragmospores, amerospores,
dictyospores, didymospores), and in few
cases spore germination were recorded on frog
skin. Detected biofilm composition was not determinate
by species and gender-specific.",
publisher = "Taylor & Francis",
journal = "European Journal of Phycology (Posters)",
title = "Green frogs skin harbours microbiota treasure",
volume = "54",
number = "sup1",
pages = "156-156",
doi = "10.1080/09670262.2019.1626628"
}

Krizmanić, J., Stupar, M., Ljaljević Grbić, M., Dimitrijević, J., Vidaković, D., Stamenković, S., Krizmanić, I.,& Breka, K.. (2019). Green frogs skin harbours microbiota treasure. in European Journal of Phycology (Posters)
Taylor & Francis., 54(sup1), 156-156.
https://doi.org/10.1080/09670262.2019.1626628

Krizmanić J, Stupar M, Ljaljević Grbić M, Dimitrijević J, Vidaković D, Stamenković S, Krizmanić I, Breka K. Green frogs skin harbours microbiota treasure. in European Journal of Phycology (Posters). 2019;54(sup1):156-156.
doi:10.1080/09670262.2019.1626628 .

Krizmanić, Jelena, Stupar, Miloš, Ljaljević Grbić, Milica, Dimitrijević, Jelica, Vidaković, Danijela, Stamenković, Srđan, Krizmanić, Imre, Breka, Katarina, "Green frogs skin harbours microbiota treasure" in European Journal of Phycology (Posters), 54, no. sup1 (2019):156-156,
https://doi.org/10.1080/09670262.2019.1626628 . .

TY  - JOUR
AU  - Janakiev, Tamara
AU  - Dimkić, Ivica
AU  - Unković, Nikola
AU  - Ljaljević Grbić, Milica
AU  - Opsenica, Dejan
AU  - Gašić, Uroš
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2019
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2019.02287/abstract
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3093
AB  - European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and it is traditionally processed into slivovitz brandy. The annual plum yields in Serbia is strongly affected by Monilinia laxa, causing brown rot in stone fruits. The fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Lepotica and Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of 11 M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist, P4/16_1 was identified as Pseudomonas synxantha, which reduced mycelial growth of M. laxa from 80 to 87.5%. The production of volatile organic compounds was observed as well. Screening for the antibiotic coding gene was positive for phenazine-1-carboxylic acid, and benzene extraction of P. synxantha-active compounds was done. The crude benzene extract exhibited 57–63% inhibition of mycelial growth. The conducted LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for use as a biocontrol agent against brown rot-causing fungi.
PB  - Frontiers
T2  - Frontiers in Microbiology
T1  - Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa
VL  - 10
SP  - 2287
DO  - 10.3389/fmicb.2019.02287
ER  - 

@article{
author = "Janakiev, Tamara and Dimkić, Ivica and Unković, Nikola and Ljaljević Grbić, Milica and Opsenica, Dejan and Gašić, Uroš and Stanković, Slaviša and Berić, Tanja",
year = "2019",
abstract = "European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and it is traditionally processed into slivovitz brandy. The annual plum yields in Serbia is strongly affected by Monilinia laxa, causing brown rot in stone fruits. The fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Lepotica and Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of 11 M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist, P4/16_1 was identified as Pseudomonas synxantha, which reduced mycelial growth of M. laxa from 80 to 87.5%. The production of volatile organic compounds was observed as well. Screening for the antibiotic coding gene was positive for phenazine-1-carboxylic acid, and benzene extraction of P. synxantha-active compounds was done. The crude benzene extract exhibited 57–63% inhibition of mycelial growth. The conducted LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for use as a biocontrol agent against brown rot-causing fungi.",
publisher = "Frontiers",
journal = "Frontiers in Microbiology",
title = "Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa",
volume = "10",
pages = "2287",
doi = "10.3389/fmicb.2019.02287"
}

Janakiev, T., Dimkić, I., Unković, N., Ljaljević Grbić, M., Opsenica, D., Gašić, U., Stanković, S.,& Berić, T.. (2019). Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa. in Frontiers in Microbiology
Frontiers., 10, 2287.
https://doi.org/10.3389/fmicb.2019.02287

Janakiev T, Dimkić I, Unković N, Ljaljević Grbić M, Opsenica D, Gašić U, Stanković S, Berić T. Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa. in Frontiers in Microbiology. 2019;10:2287.
doi:10.3389/fmicb.2019.02287 .

Janakiev, Tamara, Dimkić, Ivica, Unković, Nikola, Ljaljević Grbić, Milica, Opsenica, Dejan, Gašić, Uroš, Stanković, Slaviša, Berić, Tanja, "Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa" in Frontiers in Microbiology, 10 (2019):2287,
https://doi.org/10.3389/fmicb.2019.02287 . .

TY  - JOUR
AU  - Unković, Nikola
AU  - Popović, Slađana
AU  - Stupar, Miloš
AU  - Samolov, Elena
AU  - Subakov Simić, Gordana
AU  - Ljaljević Grbić, Milica
AU  - Vukojević, Jelena
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4748
AB  - Diversity of subaerial biofilm forming cyanobacteria, algae and fungi was investigated on 10 different substrata from greenhouse of Botanical Garden “Jevremovac”. Out of 37 documented taxa, 16 cyanobacterial and 10 algal taxa were identified. Remaining 11 taxa belong to the Kingdom of Fungi. The highest diversity of biofilm forming microorganisms, a total of 24 taxa, was detected on the corroded metal surface, while significantly lower number of taxa was recorded on other examined substrata. Cyanobacterium Porphyrosiphon sp., diatom Achnanthes sp. and green algae Chlorella sp. and Chlorococcum minutum were the most frequently encountered photosynthetic components of biofilms. In all analyzed samples, Trichoderma sp., followed by Cladosporium sp. and Rhizopus stolonifer, were the most frequently identified fungi.
AB  - Испитиван је диверзитет цијанобактерија, алги и гљива у биофилму с 10 различитих супстрата из стакленика Ботаничке баште „Јевремовац“. Од 37 документованих таксона, идентификовано је 16 цијанобактерија и 10 алги. Преосталих 11 таксона припадају „Петом царству“. Највећа разноврсност микроорганизама, укупно 24 таксона, забележена је у биофилму на кородираној металној површини, док је значајно нижи број таксона регистрован на осталим испитиваним супстратима. Цијанобактерија Porphyrosiphon sp., дијатома Achnanthes sp. и зелене алге Chlorella sp. и Chlorococcum minutum су најчешће фотосинтетичке компоненте биофилма. У свим испитиваним узорцима Trichoderma sp., заједно са Cladosporium sp. и Rhizopus stolonifer су најчешће идентификоване гљиве.
PB  - Novi Sad : Matica srpska
T2  - Zbornik Matice Srpske za Prirodne Nauke
T1  - Biofilm forming microorganisms on various substrata from greenhouse of Botanical Garden “Jevremovac”
T1  - Mikroorganizmi u sastavu biofilma s različitih supstrata staklenika Botaničke bašte “Jevremovac”
VL  - 2017
IS  - 32
SP  - 57
EP  - 67
DO  - 10.2298/ZMSPN1732057U
ER  - 

@article{
author = "Unković, Nikola and Popović, Slađana and Stupar, Miloš and Samolov, Elena and Subakov Simić, Gordana and Ljaljević Grbić, Milica and Vukojević, Jelena",
year = "2017",
abstract = "Diversity of subaerial biofilm forming cyanobacteria, algae and fungi was investigated on 10 different substrata from greenhouse of Botanical Garden “Jevremovac”. Out of 37 documented taxa, 16 cyanobacterial and 10 algal taxa were identified. Remaining 11 taxa belong to the Kingdom of Fungi. The highest diversity of biofilm forming microorganisms, a total of 24 taxa, was detected on the corroded metal surface, while significantly lower number of taxa was recorded on other examined substrata. Cyanobacterium Porphyrosiphon sp., diatom Achnanthes sp. and green algae Chlorella sp. and Chlorococcum minutum were the most frequently encountered photosynthetic components of biofilms. In all analyzed samples, Trichoderma sp., followed by Cladosporium sp. and Rhizopus stolonifer, were the most frequently identified fungi., Испитиван је диверзитет цијанобактерија, алги и гљива у биофилму с 10 различитих супстрата из стакленика Ботаничке баште „Јевремовац“. Од 37 документованих таксона, идентификовано је 16 цијанобактерија и 10 алги. Преосталих 11 таксона припадају „Петом царству“. Највећа разноврсност микроорганизама, укупно 24 таксона, забележена је у биофилму на кородираној металној површини, док је значајно нижи број таксона регистрован на осталим испитиваним супстратима. Цијанобактерија Porphyrosiphon sp., дијатома Achnanthes sp. и зелене алге Chlorella sp. и Chlorococcum minutum су најчешће фотосинтетичке компоненте биофилма. У свим испитиваним узорцима Trichoderma sp., заједно са Cladosporium sp. и Rhizopus stolonifer су најчешће идентификоване гљиве.",
publisher = "Novi Sad : Matica srpska",
journal = "Zbornik Matice Srpske za Prirodne Nauke",
title = "Biofilm forming microorganisms on various substrata from greenhouse of Botanical Garden “Jevremovac”, Mikroorganizmi u sastavu biofilma s različitih supstrata staklenika Botaničke bašte “Jevremovac”",
volume = "2017",
number = "32",
pages = "57-67",
doi = "10.2298/ZMSPN1732057U"
}

Unković, N., Popović, S., Stupar, M., Samolov, E., Subakov Simić, G., Ljaljević Grbić, M.,& Vukojević, J.. (2017). Biofilm forming microorganisms on various substrata from greenhouse of Botanical Garden “Jevremovac”. in Zbornik Matice Srpske za Prirodne Nauke
Novi Sad : Matica srpska., 2017(32), 57-67.
https://doi.org/10.2298/ZMSPN1732057U

Unković N, Popović S, Stupar M, Samolov E, Subakov Simić G, Ljaljević Grbić M, Vukojević J. Biofilm forming microorganisms on various substrata from greenhouse of Botanical Garden “Jevremovac”. in Zbornik Matice Srpske za Prirodne Nauke. 2017;2017(32):57-67.
doi:10.2298/ZMSPN1732057U .

Unković, Nikola, Popović, Slađana, Stupar, Miloš, Samolov, Elena, Subakov Simić, Gordana, Ljaljević Grbić, Milica, Vukojević, Jelena, "Biofilm forming microorganisms on various substrata from greenhouse of Botanical Garden “Jevremovac”" in Zbornik Matice Srpske za Prirodne Nauke, 2017, no. 32 (2017):57-67,
https://doi.org/10.2298/ZMSPN1732057U . .

TY  - CONF
AU  - Krizmanić, Jelena
AU  - Vidaković, Danijela
AU  - Stupar, Miloš
AU  - Ljaljević Grbić, Milica
AU  - Krizmanić, Imre
AU  - Stamenković, Srđan
AU  - Breka, Katarina
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4646
AB  - It is known that diatoms may be present on the skin of various animals (whales, dolphins, manatees, turtles…) (Frankovich et al. 2015, Wetzel et al. 2012). In our study diatoms were collected from the skin of two green frogs’ species: Pelophylax esculentus and P. ridibundus. Studied frogs were captured from the Special Nature Reserve “Deliblatska Pescara” at the locality Stevanove ravnice (Vojvodina province, Serbia). Two sampling methods were performed: (1) nonaggressive adhesive tape method (Urzì and de Leo 2001) and (2) scraping by toothbrush. In laboratory conditions, samples taken by the first method were stained with a drop of Lactophenol Cotton Blue and put on slides to be analyzed. Samples taken by the second method were treated by hot HCl and KMnO4 (Taylor et al. 2005) in order to obtain permanent slides. Light microscope observations and micrographs were made by Zeiss AxioImagerM.1 microscope with DIC optics (x1000 and x1600 magnification) and AxioVision 4.8 software.

The present study describes diversity of diatoms from the green frog skin. According to literature data no studies have been published on diatoms living on the frog skin.

Analysis of adhesive tape samples confirmed the presence of live diatom cells on the frog skin. Among them the most abundant were taxa from the genera Cocconeis, Epithemia, Gomphonema, Navicula and Rhopalodia. Presence of c. 30 diatom genera, such as Amphora, Craticula, Cymbella, Cymbopleura, Cymatopleura, Diploneis, Encyonema, Epithemia, Fallacia, Gomphonema, Luticola, Navicula, Neidium, Nitzschia, Pinnularia, Planothidium, Rhopalodia, Staurosira, Surirella, etc., was observed on permanent slides. The most dominant taxa were: Geissleria decussis sensu lato, Placoneis sp., Lemnicola hungarica, Cocconeis placentula var. lineate and Sellaphora bacillum.

We consider that the great diversity and abundance of diatoms is consequence of their transfer from the environment (mud and macrophytes from eutrophic ponds) onto mucous frog skin. Highly mucous skin is an excellent environment which enables primary conditions for the survival of diatoms. Future research, especially under laboratory conditions, could show whether the frog skin is permanent or temporary diatom habitat.
PB  - Prague : Charles University in Prague
C3  - Programme and abstract book - 11th Central European Diatom Meeting
T1  - Diatoms on the green frogs skin (Pelophylax esculentus and P. ridibundus)
SP  - 114
EP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_cer_4646
ER  - 

@conference{
author = "Krizmanić, Jelena and Vidaković, Danijela and Stupar, Miloš and Ljaljević Grbić, Milica and Krizmanić, Imre and Stamenković, Srđan and Breka, Katarina",
year = "2017",
abstract = "It is known that diatoms may be present on the skin of various animals (whales, dolphins, manatees, turtles…) (Frankovich et al. 2015, Wetzel et al. 2012). In our study diatoms were collected from the skin of two green frogs’ species: Pelophylax esculentus and P. ridibundus. Studied frogs were captured from the Special Nature Reserve “Deliblatska Pescara” at the locality Stevanove ravnice (Vojvodina province, Serbia). Two sampling methods were performed: (1) nonaggressive adhesive tape method (Urzì and de Leo 2001) and (2) scraping by toothbrush. In laboratory conditions, samples taken by the first method were stained with a drop of Lactophenol Cotton Blue and put on slides to be analyzed. Samples taken by the second method were treated by hot HCl and KMnO4 (Taylor et al. 2005) in order to obtain permanent slides. Light microscope observations and micrographs were made by Zeiss AxioImagerM.1 microscope with DIC optics (x1000 and x1600 magnification) and AxioVision 4.8 software.

The present study describes diversity of diatoms from the green frog skin. According to literature data no studies have been published on diatoms living on the frog skin.

Analysis of adhesive tape samples confirmed the presence of live diatom cells on the frog skin. Among them the most abundant were taxa from the genera Cocconeis, Epithemia, Gomphonema, Navicula and Rhopalodia. Presence of c. 30 diatom genera, such as Amphora, Craticula, Cymbella, Cymbopleura, Cymatopleura, Diploneis, Encyonema, Epithemia, Fallacia, Gomphonema, Luticola, Navicula, Neidium, Nitzschia, Pinnularia, Planothidium, Rhopalodia, Staurosira, Surirella, etc., was observed on permanent slides. The most dominant taxa were: Geissleria decussis sensu lato, Placoneis sp., Lemnicola hungarica, Cocconeis placentula var. lineate and Sellaphora bacillum.

We consider that the great diversity and abundance of diatoms is consequence of their transfer from the environment (mud and macrophytes from eutrophic ponds) onto mucous frog skin. Highly mucous skin is an excellent environment which enables primary conditions for the survival of diatoms. Future research, especially under laboratory conditions, could show whether the frog skin is permanent or temporary diatom habitat.",
publisher = "Prague : Charles University in Prague",
journal = "Programme and abstract book - 11th Central European Diatom Meeting",
title = "Diatoms on the green frogs skin (Pelophylax esculentus and P. ridibundus)",
pages = "114-114",
url = "https://hdl.handle.net/21.15107/rcub_cer_4646"
}

Krizmanić, J., Vidaković, D., Stupar, M., Ljaljević Grbić, M., Krizmanić, I., Stamenković, S.,& Breka, K.. (2017). Diatoms on the green frogs skin (Pelophylax esculentus and P. ridibundus). in Programme and abstract book - 11th Central European Diatom Meeting
Prague : Charles University in Prague., 114-114.
https://hdl.handle.net/21.15107/rcub_cer_4646

Krizmanić J, Vidaković D, Stupar M, Ljaljević Grbić M, Krizmanić I, Stamenković S, Breka K. Diatoms on the green frogs skin (Pelophylax esculentus and P. ridibundus). in Programme and abstract book - 11th Central European Diatom Meeting. 2017;:114-114.
https://hdl.handle.net/21.15107/rcub_cer_4646 .

Krizmanić, Jelena, Vidaković, Danijela, Stupar, Miloš, Ljaljević Grbić, Milica, Krizmanić, Imre, Stamenković, Srđan, Breka, Katarina, "Diatoms on the green frogs skin (Pelophylax esculentus and P. ridibundus)" in Programme and abstract book - 11th Central European Diatom Meeting (2017):114-114,
https://hdl.handle.net/21.15107/rcub_cer_4646 .