TY  - PAT
AU  - Josić, Djuro
AU  - Begić, Marija
AU  - Andjelković, Uroš
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6940
AB  - A process for the purification of Prothrombin Complex Concentrate (PCC) from complete plasma or cryo-poor plasma using chromatography, the method comprising performing an initial sample displacement chromatography step of complete plasma or cryo-poor plasma, in particular undiluted cryo-poor plasma, on an anion exchanger to obtain a first fraction enriched in PCC.A fraction comprising PCC and factor IX obtainable by the process of the invention.
PB  - European Patent Office
T2  - European patent office
T1  - A process for the purification of prothrombin complex concentrate (PCC) and FIX from complete plasma or cryo-poor plasma
IS  - EP3945095A1
UR  - https://hdl.handle.net/21.15107/rcub_cer_6940
ER  - 

@misc{
author = "Josić, Djuro and Begić, Marija and Andjelković, Uroš",
year = "2022",
abstract = "A process for the purification of Prothrombin Complex Concentrate (PCC) from complete plasma or cryo-poor plasma using chromatography, the method comprising performing an initial sample displacement chromatography step of complete plasma or cryo-poor plasma, in particular undiluted cryo-poor plasma, on an anion exchanger to obtain a first fraction enriched in PCC.A fraction comprising PCC and factor IX obtainable by the process of the invention.",
publisher = "European Patent Office",
journal = "European patent office",
title = "A process for the purification of prothrombin complex concentrate (PCC) and FIX from complete plasma or cryo-poor plasma",
number = "EP3945095A1",
url = "https://hdl.handle.net/21.15107/rcub_cer_6940"
}

Josić, D., Begić, M.,& Andjelković, U.. (2022). A process for the purification of prothrombin complex concentrate (PCC) and FIX from complete plasma or cryo-poor plasma. in European patent office
European Patent Office.(EP3945095A1).
https://hdl.handle.net/21.15107/rcub_cer_6940

Josić D, Begić M, Andjelković U. A process for the purification of prothrombin complex concentrate (PCC) and FIX from complete plasma or cryo-poor plasma. in European patent office. 2022;(EP3945095A1).
https://hdl.handle.net/21.15107/rcub_cer_6940 .

Josić, Djuro, Begić, Marija, Andjelković, Uroš, "A process for the purification of prothrombin complex concentrate (PCC) and FIX from complete plasma or cryo-poor plasma" in European patent office, no. EP3945095A1 (2022),
https://hdl.handle.net/21.15107/rcub_cer_6940 .

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Gudelj, Ivan
AU  - Klarić, Thomas
AU  - Hinneburg, Hannes
AU  - Vinković, Marijana
AU  - Wittine, Karlo
AU  - Dovezenski, Nebojša
AU  - Vikić-Topić, Dražen
AU  - Lauc, Gordan
AU  - Vujčić, Zoran
AU  - Josić, Đuro
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3732
AB  - Invertases are glycosidases applied for synthesis of alkyl glycosides that are important and effective surfactants. Stability of invertases in the environment with increased content of organic solvent is crucial for increase of productivity of glycosidases. Their stability
is significantly influenced by N-glycosylation. However, yeast N-glycosylation pathways may synthesize plethora of N-glycan structures. A total natural crude mixture of invertase glycoforms (EINV) extracted from Saccharomyces cerevisiae was subfractionated
by anion-exchange chromatography on industrial monolithic supports to obtain different glycoforms (EINV1–EINV3). Separated glycoforms exhibited different stabilities in wateralcohol solutions that are in direct correlation with the amount of phosphate bound to N-glycans. Observed differences in stability of different invertase glycoforms were used to improve productivity of methyl β-d-fructofuranoside (MF) synthesis. The efficiency and yield of MF synthesis were improved more than 50% when the most stabile glycoform bearing the lowest amount of phosphorylated N-glycans is selected and utilized. These data underline the importance of analysis of glycan structures attached to glycoproteins, demonstrate different impact of N-glycans on the surface charge and enzyme stability in
regard to particular reaction environment, and provide a platform for improvement of yield of industrial enzymatic synthesis by chromatographic selection of glycoforms on monolithic supports.
PB  - Wiley
T2  - Electrophoresis
T1  - Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase
VL  - 42
IS  - 24
SP  - 2626
EP  - 2636
DO  - 10.1002/elps.202000092
ER  - 

@article{
author = "Anđelković, Uroš and Gudelj, Ivan and Klarić, Thomas and Hinneburg, Hannes and Vinković, Marijana and Wittine, Karlo and Dovezenski, Nebojša and Vikić-Topić, Dražen and Lauc, Gordan and Vujčić, Zoran and Josić, Đuro",
year = "2021",
abstract = "Invertases are glycosidases applied for synthesis of alkyl glycosides that are important and effective surfactants. Stability of invertases in the environment with increased content of organic solvent is crucial for increase of productivity of glycosidases. Their stability
is significantly influenced by N-glycosylation. However, yeast N-glycosylation pathways may synthesize plethora of N-glycan structures. A total natural crude mixture of invertase glycoforms (EINV) extracted from Saccharomyces cerevisiae was subfractionated
by anion-exchange chromatography on industrial monolithic supports to obtain different glycoforms (EINV1–EINV3). Separated glycoforms exhibited different stabilities in wateralcohol solutions that are in direct correlation with the amount of phosphate bound to N-glycans. Observed differences in stability of different invertase glycoforms were used to improve productivity of methyl β-d-fructofuranoside (MF) synthesis. The efficiency and yield of MF synthesis were improved more than 50% when the most stabile glycoform bearing the lowest amount of phosphorylated N-glycans is selected and utilized. These data underline the importance of analysis of glycan structures attached to glycoproteins, demonstrate different impact of N-glycans on the surface charge and enzyme stability in
regard to particular reaction environment, and provide a platform for improvement of yield of industrial enzymatic synthesis by chromatographic selection of glycoforms on monolithic supports.",
publisher = "Wiley",
journal = "Electrophoresis",
title = "Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase",
volume = "42",
number = "24",
pages = "2626-2636",
doi = "10.1002/elps.202000092"
}

Anđelković, U., Gudelj, I., Klarić, T., Hinneburg, H., Vinković, M., Wittine, K., Dovezenski, N., Vikić-Topić, D., Lauc, G., Vujčić, Z.,& Josić, Đ.. (2021). Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase. in Electrophoresis
Wiley., 42(24), 2626-2636.
https://doi.org/10.1002/elps.202000092

Anđelković U, Gudelj I, Klarić T, Hinneburg H, Vinković M, Wittine K, Dovezenski N, Vikić-Topić D, Lauc G, Vujčić Z, Josić Đ. Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase. in Electrophoresis. 2021;42(24):2626-2636.
doi:10.1002/elps.202000092 .

Anđelković, Uroš, Gudelj, Ivan, Klarić, Thomas, Hinneburg, Hannes, Vinković, Marijana, Wittine, Karlo, Dovezenski, Nebojša, Vikić-Topić, Dražen, Lauc, Gordan, Vujčić, Zoran, Josić, Đuro, "Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase" in Electrophoresis, 42, no. 24 (2021):2626-2636,
https://doi.org/10.1002/elps.202000092 . .

TY  - JOUR
AU  - Srajer-Gajdošik, Martina
AU  - Anđelković, Uroš
AU  - Gašo Sokač, Dajana
AU  - Pavlović, Hrvoje
AU  - Shevchuk, Olga
AU  - Martinović, Tamara
AU  - Clifton, James
AU  - Begić, Marija
AU  - Josić, Djuro
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6923
AB  - Proteomic analysis of foodborne pathogen Listeria monocytogenes after treatment with three disinfectants based on ammonium salts of pyridoxal oxime (POD) reveal perturbation of cellular processes. These inhibitors caused disturbance in the synthesis of plasma membrane proteins and cell wall proteoglycans. Some of key proteins and proteoglycans from these two groups that are important for bacterial growth are down-regulated. Additionally, we demonstrated that the main bacterial toxin Listeriolysin O (LLO) is significantly down-regulated after treatment with each of three investigated inhibitors. These investigations confirm already postulated mechanism of action of POD-based inhibitors that results in disturbance of key cell surface proteins and proteoglycans in Gram-positive bacteria. Additionally, the use of some proteins such as LLO, as potential biomarker candidates of food poisoning with this bacterium is discussed.
PB  - Elsevier
T2  - Food Research International
T1  - Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O
VL  - 131
SP  - 108951
DO  - 10.1016/j.foodres.2019.108951
ER  - 

@article{
author = "Srajer-Gajdošik, Martina and Anđelković, Uroš and Gašo Sokač, Dajana and Pavlović, Hrvoje and Shevchuk, Olga and Martinović, Tamara and Clifton, James and Begić, Marija and Josić, Djuro",
year = "2020",
abstract = "Proteomic analysis of foodborne pathogen Listeria monocytogenes after treatment with three disinfectants based on ammonium salts of pyridoxal oxime (POD) reveal perturbation of cellular processes. These inhibitors caused disturbance in the synthesis of plasma membrane proteins and cell wall proteoglycans. Some of key proteins and proteoglycans from these two groups that are important for bacterial growth are down-regulated. Additionally, we demonstrated that the main bacterial toxin Listeriolysin O (LLO) is significantly down-regulated after treatment with each of three investigated inhibitors. These investigations confirm already postulated mechanism of action of POD-based inhibitors that results in disturbance of key cell surface proteins and proteoglycans in Gram-positive bacteria. Additionally, the use of some proteins such as LLO, as potential biomarker candidates of food poisoning with this bacterium is discussed.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O",
volume = "131",
pages = "108951",
doi = "10.1016/j.foodres.2019.108951"
}

Srajer-Gajdošik, M., Anđelković, U., Gašo Sokač, D., Pavlović, H., Shevchuk, O., Martinović, T., Clifton, J., Begić, M.,& Josić, D.. (2020). Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O. in Food Research International
Elsevier., 131, 108951.
https://doi.org/10.1016/j.foodres.2019.108951

Srajer-Gajdošik M, Anđelković U, Gašo Sokač D, Pavlović H, Shevchuk O, Martinović T, Clifton J, Begić M, Josić D. Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O. in Food Research International. 2020;131:108951.
doi:10.1016/j.foodres.2019.108951 .

Srajer-Gajdošik, Martina, Anđelković, Uroš, Gašo Sokač, Dajana, Pavlović, Hrvoje, Shevchuk, Olga, Martinović, Tamara, Clifton, James, Begić, Marija, Josić, Djuro, "Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O" in Food Research International, 131 (2020):108951,
https://doi.org/10.1016/j.foodres.2019.108951 . .

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Gudelj, Ivan
AU  - Klarić, Thomas
AU  - Hinneburg, Hannes
AU  - Vinković, Marijana
AU  - Wittine, Karlo
AU  - Dovezenski, Nebojša
AU  - Vikić-Topić, Dražen
AU  - Lauc, Gordan
AU  - Vujčić, Zoran
AU  - Josić, Đuro
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3989
AB  - Invertases are glycosidases applied for synthesis of alkyl glycosides that are important and effective surfactants. Stability of invertases in the environment with increased content of organic solvent is crucial for increase of productivity of glycosidases. Their stabilityis significantly influenced by N-glycosylation. However, yeast N-glycosylation pathways may synthesize plethora of N-glycan structures. A total natural crude mixture of invertase glycoforms (EINV) extracted from Saccharomyces cerevisiae was subfractionatedby anion-exchange chromatography on industrial monolithic supports to obtain different glycoforms (EINV1–EINV3). Separated glycoforms exhibited different stabilities in wateralcohol solutions that are in direct correlation with the amount of phosphate bound to N-glycans. Observed differences in stability of different invertase glycoforms were used to improve productivity of methyl β-d-fructofuranoside (MF) synthesis. The efficiency and yield of MF synthesis were improved more than 50% when the most stabile glycoform bearing the lowest amount of phosphorylated N-glycans is selected and utilized. These data underline the importance of analysis of glycan structures attached to glycoproteins, demonstrate different impact of N-glycans on the surface charge and enzyme stability inregard to particular reaction environment, and provide a platform for improvement of yield of industrial enzymatic synthesis by chromatographic selection of glycoforms on monolithic supports.
PB  - Wiley
T2  - Electrophoresis
T1  - Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase
DO  - 10.1002/elps.202000092
ER  - 

@article{
author = "Anđelković, Uroš and Gudelj, Ivan and Klarić, Thomas and Hinneburg, Hannes and Vinković, Marijana and Wittine, Karlo and Dovezenski, Nebojša and Vikić-Topić, Dražen and Lauc, Gordan and Vujčić, Zoran and Josić, Đuro",
year = "2020",
abstract = "Invertases are glycosidases applied for synthesis of alkyl glycosides that are important and effective surfactants. Stability of invertases in the environment with increased content of organic solvent is crucial for increase of productivity of glycosidases. Their stabilityis significantly influenced by N-glycosylation. However, yeast N-glycosylation pathways may synthesize plethora of N-glycan structures. A total natural crude mixture of invertase glycoforms (EINV) extracted from Saccharomyces cerevisiae was subfractionatedby anion-exchange chromatography on industrial monolithic supports to obtain different glycoforms (EINV1–EINV3). Separated glycoforms exhibited different stabilities in wateralcohol solutions that are in direct correlation with the amount of phosphate bound to N-glycans. Observed differences in stability of different invertase glycoforms were used to improve productivity of methyl β-d-fructofuranoside (MF) synthesis. The efficiency and yield of MF synthesis were improved more than 50% when the most stabile glycoform bearing the lowest amount of phosphorylated N-glycans is selected and utilized. These data underline the importance of analysis of glycan structures attached to glycoproteins, demonstrate different impact of N-glycans on the surface charge and enzyme stability inregard to particular reaction environment, and provide a platform for improvement of yield of industrial enzymatic synthesis by chromatographic selection of glycoforms on monolithic supports.",
publisher = "Wiley",
journal = "Electrophoresis",
title = "Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase",
doi = "10.1002/elps.202000092"
}

Anđelković, U., Gudelj, I., Klarić, T., Hinneburg, H., Vinković, M., Wittine, K., Dovezenski, N., Vikić-Topić, D., Lauc, G., Vujčić, Z.,& Josić, Đ.. (2020). Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase. in Electrophoresis
Wiley..
https://doi.org/10.1002/elps.202000092

Anđelković U, Gudelj I, Klarić T, Hinneburg H, Vinković M, Wittine K, Dovezenski N, Vikić-Topić D, Lauc G, Vujčić Z, Josić Đ. Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase. in Electrophoresis. 2020;.
doi:10.1002/elps.202000092 .

Anđelković, Uroš, Gudelj, Ivan, Klarić, Thomas, Hinneburg, Hannes, Vinković, Marijana, Wittine, Karlo, Dovezenski, Nebojša, Vikić-Topić, Dražen, Lauc, Gordan, Vujčić, Zoran, Josić, Đuro, "Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase" in Electrophoresis (2020),
https://doi.org/10.1002/elps.202000092 . .

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Josić, Djuro
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2287
AB  - Background.: As a comprehensive discipline that studies food and nutrition, foodomics requires reliable qualitative and quantitative information about the food proteome component in order to extract new integrative information from the complex multivariable space of omics. This new information is necessary to achieve a higher level of understanding of processes in food science and technology, consequently new functions of food and improved markers of food quality and safety and completely transform concept of food safety. Scope and approach: We are making an effort to present mass spectrometry (MS) based proteomic approaches that are being utilized in different proteomic studies, not necessarily in the field of foodomics, which are important and have the potential to advance this field. Current analytical capabilities of MS-based proteomics together with sample preparation procedures and quantification strategies, and recent technical developments were presented. Key findings and conclusions: MS-based proteomics enables the analysis of different aspects of proteins and provides a variety of approaches for reliable quantification of individual proteinsand/or food proteome. This is a complex field and its successful implementation requires a dedicated analyst, a thorough design of sample preparation procedure, the selection of an MS technique and approach, an adequate type of mass spectrometer, a thorough data analysis and validation. Improvements in the technology of mass spectrometers are continuously expanding capabilities of MS-based proteomics.
PB  - Elsevier Science London, London
T2  - Trends in Food Science & Technology
T1  - Mass spectrometry based proteomics as foodomics tool in research and assurance of food quality and safety
VL  - 77
SP  - 100
EP  - 119
DO  - 10.1016/j.tifs.2018.04.008
ER  - 

@article{
author = "Anđelković, Uroš and Josić, Djuro",
year = "2018",
abstract = "Background.: As a comprehensive discipline that studies food and nutrition, foodomics requires reliable qualitative and quantitative information about the food proteome component in order to extract new integrative information from the complex multivariable space of omics. This new information is necessary to achieve a higher level of understanding of processes in food science and technology, consequently new functions of food and improved markers of food quality and safety and completely transform concept of food safety. Scope and approach: We are making an effort to present mass spectrometry (MS) based proteomic approaches that are being utilized in different proteomic studies, not necessarily in the field of foodomics, which are important and have the potential to advance this field. Current analytical capabilities of MS-based proteomics together with sample preparation procedures and quantification strategies, and recent technical developments were presented. Key findings and conclusions: MS-based proteomics enables the analysis of different aspects of proteins and provides a variety of approaches for reliable quantification of individual proteinsand/or food proteome. This is a complex field and its successful implementation requires a dedicated analyst, a thorough design of sample preparation procedure, the selection of an MS technique and approach, an adequate type of mass spectrometer, a thorough data analysis and validation. Improvements in the technology of mass spectrometers are continuously expanding capabilities of MS-based proteomics.",
publisher = "Elsevier Science London, London",
journal = "Trends in Food Science & Technology",
title = "Mass spectrometry based proteomics as foodomics tool in research and assurance of food quality and safety",
volume = "77",
pages = "100-119",
doi = "10.1016/j.tifs.2018.04.008"
}

Anđelković, U.,& Josić, D.. (2018). Mass spectrometry based proteomics as foodomics tool in research and assurance of food quality and safety. in Trends in Food Science & Technology
Elsevier Science London, London., 77, 100-119.
https://doi.org/10.1016/j.tifs.2018.04.008

Anđelković U, Josić D. Mass spectrometry based proteomics as foodomics tool in research and assurance of food quality and safety. in Trends in Food Science & Technology. 2018;77:100-119.
doi:10.1016/j.tifs.2018.04.008 .

Anđelković, Uroš, Josić, Djuro, "Mass spectrometry based proteomics as foodomics tool in research and assurance of food quality and safety" in Trends in Food Science & Technology, 77 (2018):100-119,
https://doi.org/10.1016/j.tifs.2018.04.008 . .

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Josić, Djuro
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4280
AB  - Background.: As a comprehensive discipline that studies food and nutrition, foodomics requires reliable qualitative and quantitative information about the food proteome component in order to extract new integrative information from the complex multivariable space of omics. This new information is necessary to achieve a higher level of understanding of processes in food science and technology, consequently new functions of food and improved markers of food quality and safety and completely transform concept of food safety. Scope and approach: We are making an effort to present mass spectrometry (MS) based proteomic approaches that are being utilized in different proteomic studies, not necessarily in the field of foodomics, which are important and have the potential to advance this field. Current analytical capabilities of MS-based proteomics together with sample preparation procedures and quantification strategies, and recent technical developments were presented. Key findings and conclusions: MS-based proteomics enables the analysis of different aspects of proteins and provides a variety of approaches for reliable quantification of individual proteinsand/or food proteome. This is a complex field and its successful implementation requires a dedicated analyst, a thorough design of sample preparation procedure, the selection of an MS technique and approach, an adequate type of mass spectrometer, a thorough data analysis and validation. Improvements in the technology of mass spectrometers are continuously expanding capabilities of MS-based proteomics.
PB  - Elsevier Science London, London
T2  - Trends in Food Science & Technology
T1  - Mass spectrometry based proteomics as foodomics tool in research and assurance of food quality and safety
VL  - 77
SP  - 100
EP  - 119
DO  - 10.1016/j.tifs.2018.04.008
ER  - 

@article{
author = "Anđelković, Uroš and Josić, Djuro",
year = "2018",
abstract = "Background.: As a comprehensive discipline that studies food and nutrition, foodomics requires reliable qualitative and quantitative information about the food proteome component in order to extract new integrative information from the complex multivariable space of omics. This new information is necessary to achieve a higher level of understanding of processes in food science and technology, consequently new functions of food and improved markers of food quality and safety and completely transform concept of food safety. Scope and approach: We are making an effort to present mass spectrometry (MS) based proteomic approaches that are being utilized in different proteomic studies, not necessarily in the field of foodomics, which are important and have the potential to advance this field. Current analytical capabilities of MS-based proteomics together with sample preparation procedures and quantification strategies, and recent technical developments were presented. Key findings and conclusions: MS-based proteomics enables the analysis of different aspects of proteins and provides a variety of approaches for reliable quantification of individual proteinsand/or food proteome. This is a complex field and its successful implementation requires a dedicated analyst, a thorough design of sample preparation procedure, the selection of an MS technique and approach, an adequate type of mass spectrometer, a thorough data analysis and validation. Improvements in the technology of mass spectrometers are continuously expanding capabilities of MS-based proteomics.",
publisher = "Elsevier Science London, London",
journal = "Trends in Food Science & Technology",
title = "Mass spectrometry based proteomics as foodomics tool in research and assurance of food quality and safety",
volume = "77",
pages = "100-119",
doi = "10.1016/j.tifs.2018.04.008"
}

Anđelković, U.,& Josić, D.. (2018). Mass spectrometry based proteomics as foodomics tool in research and assurance of food quality and safety. in Trends in Food Science & Technology
Elsevier Science London, London., 77, 100-119.
https://doi.org/10.1016/j.tifs.2018.04.008

Anđelković U, Josić D. Mass spectrometry based proteomics as foodomics tool in research and assurance of food quality and safety. in Trends in Food Science & Technology. 2018;77:100-119.
doi:10.1016/j.tifs.2018.04.008 .

Anđelković, Uroš, Josić, Djuro, "Mass spectrometry based proteomics as foodomics tool in research and assurance of food quality and safety" in Trends in Food Science & Technology, 77 (2018):100-119,
https://doi.org/10.1016/j.tifs.2018.04.008 . .

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Gavrović-Jankulović, Marija
AU  - Martinović, Tamara
AU  - Josić, Djuro
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2113
AB  - Use of foodomics, mostly proteomic and genomic based methods, for study of allergens in food is presented. Immunological methods and nucleic acid-based methods are still most frequently used for diagnosis of allergies and for qualitative and quantitative determination of food allergens. They are sensitive, and can be used for the determination of allergens in trace concentrations. However, lack of specificity and cross-reaction of some antibodies can still be a relevant source of bias. The epitopes of protein allergens with posttranslational modifications and their changes originated during food processing cannot be traced by use of nucleic acid-based strategies. Recent developments of both antibody and nucleic acid-based biosensors, their miniaturization and increasing application of nanotechnology, significantly supported further use of both strategies. Regarding accuracy, reliability and sensitivity, mass spectrometry-based methods bring important advantage over both above presented strategies. Furthermore, the increasing use of mass spectrometry (MS) is discussed. Combined with proper sample preparation, liquid chromatography (LC) and/or different electrophoretic methods, targeted approach in mass spectrometry-based allergen analysis brings an additional strategic advance. However, MS is still rarely used for high-throughput analyses and detection and quantification of allergens for the reasons of price and relatively long time necessary for analysis. Recent developments of new high-resolution instruments are encouraging and enable development in the direction of a high-throughput strategy. Consequently, fast, very sensitive, reliable and accurate detection and quantification of allergens in highly complex samples such as food matrices, and the use of MS in routine determination of allergens can be reached in near future.
PB  - Elsevier Sci Ltd, Oxford
T2  - Trac-Trends in Analytical Chemistry
T1  - Omics methods as a tool for investigation of food allergies
VL  - 96
SP  - 107
EP  - 115
DO  - 10.1016/j.trac.2017.07.011
ER  - 

@article{
author = "Anđelković, Uroš and Gavrović-Jankulović, Marija and Martinović, Tamara and Josić, Djuro",
year = "2017",
abstract = "Use of foodomics, mostly proteomic and genomic based methods, for study of allergens in food is presented. Immunological methods and nucleic acid-based methods are still most frequently used for diagnosis of allergies and for qualitative and quantitative determination of food allergens. They are sensitive, and can be used for the determination of allergens in trace concentrations. However, lack of specificity and cross-reaction of some antibodies can still be a relevant source of bias. The epitopes of protein allergens with posttranslational modifications and their changes originated during food processing cannot be traced by use of nucleic acid-based strategies. Recent developments of both antibody and nucleic acid-based biosensors, their miniaturization and increasing application of nanotechnology, significantly supported further use of both strategies. Regarding accuracy, reliability and sensitivity, mass spectrometry-based methods bring important advantage over both above presented strategies. Furthermore, the increasing use of mass spectrometry (MS) is discussed. Combined with proper sample preparation, liquid chromatography (LC) and/or different electrophoretic methods, targeted approach in mass spectrometry-based allergen analysis brings an additional strategic advance. However, MS is still rarely used for high-throughput analyses and detection and quantification of allergens for the reasons of price and relatively long time necessary for analysis. Recent developments of new high-resolution instruments are encouraging and enable development in the direction of a high-throughput strategy. Consequently, fast, very sensitive, reliable and accurate detection and quantification of allergens in highly complex samples such as food matrices, and the use of MS in routine determination of allergens can be reached in near future.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Trac-Trends in Analytical Chemistry",
title = "Omics methods as a tool for investigation of food allergies",
volume = "96",
pages = "107-115",
doi = "10.1016/j.trac.2017.07.011"
}

Anđelković, U., Gavrović-Jankulović, M., Martinović, T.,& Josić, D.. (2017). Omics methods as a tool for investigation of food allergies. in Trac-Trends in Analytical Chemistry
Elsevier Sci Ltd, Oxford., 96, 107-115.
https://doi.org/10.1016/j.trac.2017.07.011

Anđelković U, Gavrović-Jankulović M, Martinović T, Josić D. Omics methods as a tool for investigation of food allergies. in Trac-Trends in Analytical Chemistry. 2017;96:107-115.
doi:10.1016/j.trac.2017.07.011 .

Anđelković, Uroš, Gavrović-Jankulović, Marija, Martinović, Tamara, Josić, Djuro, "Omics methods as a tool for investigation of food allergies" in Trac-Trends in Analytical Chemistry, 96 (2017):107-115,
https://doi.org/10.1016/j.trac.2017.07.011 . .

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Gavrović-Jankulović, Marija
AU  - Martinović, Tamara
AU  - Josić, Djuro
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2937
AB  - Use of foodomics, mostly proteomic and genomic based methods, for study of allergens in food is presented. Immunological methods and nucleic acid-based methods are still most frequently used for diagnosis of allergies and for qualitative and quantitative determination of food allergens. They are sensitive, and can be used for the determination of allergens in trace concentrations. However, lack of specificity and cross-reaction of some antibodies can still be a relevant source of bias. The epitopes of protein allergens with posttranslational modifications and their changes originated during food processing cannot be traced by use of nucleic acid-based strategies. Recent developments of both antibody and nucleic acid-based biosensors, their miniaturization and increasing application of nanotechnology, significantly supported further use of both strategies. Regarding accuracy, reliability and sensitivity, mass spectrometry-based methods bring important advantage over both above presented strategies. Furthermore, the increasing use of mass spectrometry (MS) is discussed. Combined with proper sample preparation, liquid chromatography (LC) and/or different electrophoretic methods, targeted approach in mass spectrometry-based allergen analysis brings an additional strategic advance. However, MS is still rarely used for high-throughput analyses and detection and quantification of allergens for the reasons of price and relatively long time necessary for analysis. Recent developments of new high-resolution instruments are encouraging and enable development in the direction of a high-throughput strategy. Consequently, fast, very sensitive, reliable and accurate detection and quantification of allergens in highly complex samples such as food matrices, and the use of MS in routine determination of allergens can be reached in near future.
PB  - Elsevier
T2  - Trac-Trends in Analytical Chemistry
T1  - Omics methods as a tool for investigation of food allergies
VL  - 96
SP  - 107
EP  - 115
DO  - 10.1016/j.trac.2017.07.011
ER  - 

@article{
author = "Anđelković, Uroš and Gavrović-Jankulović, Marija and Martinović, Tamara and Josić, Djuro",
year = "2017",
abstract = "Use of foodomics, mostly proteomic and genomic based methods, for study of allergens in food is presented. Immunological methods and nucleic acid-based methods are still most frequently used for diagnosis of allergies and for qualitative and quantitative determination of food allergens. They are sensitive, and can be used for the determination of allergens in trace concentrations. However, lack of specificity and cross-reaction of some antibodies can still be a relevant source of bias. The epitopes of protein allergens with posttranslational modifications and their changes originated during food processing cannot be traced by use of nucleic acid-based strategies. Recent developments of both antibody and nucleic acid-based biosensors, their miniaturization and increasing application of nanotechnology, significantly supported further use of both strategies. Regarding accuracy, reliability and sensitivity, mass spectrometry-based methods bring important advantage over both above presented strategies. Furthermore, the increasing use of mass spectrometry (MS) is discussed. Combined with proper sample preparation, liquid chromatography (LC) and/or different electrophoretic methods, targeted approach in mass spectrometry-based allergen analysis brings an additional strategic advance. However, MS is still rarely used for high-throughput analyses and detection and quantification of allergens for the reasons of price and relatively long time necessary for analysis. Recent developments of new high-resolution instruments are encouraging and enable development in the direction of a high-throughput strategy. Consequently, fast, very sensitive, reliable and accurate detection and quantification of allergens in highly complex samples such as food matrices, and the use of MS in routine determination of allergens can be reached in near future.",
publisher = "Elsevier",
journal = "Trac-Trends in Analytical Chemistry",
title = "Omics methods as a tool for investigation of food allergies",
volume = "96",
pages = "107-115",
doi = "10.1016/j.trac.2017.07.011"
}

Anđelković, U., Gavrović-Jankulović, M., Martinović, T.,& Josić, D.. (2017). Omics methods as a tool for investigation of food allergies. in Trac-Trends in Analytical Chemistry
Elsevier., 96, 107-115.
https://doi.org/10.1016/j.trac.2017.07.011

Anđelković U, Gavrović-Jankulović M, Martinović T, Josić D. Omics methods as a tool for investigation of food allergies. in Trac-Trends in Analytical Chemistry. 2017;96:107-115.
doi:10.1016/j.trac.2017.07.011 .

Anđelković, Uroš, Gavrović-Jankulović, Marija, Martinović, Tamara, Josić, Djuro, "Omics methods as a tool for investigation of food allergies" in Trac-Trends in Analytical Chemistry, 96 (2017):107-115,
https://doi.org/10.1016/j.trac.2017.07.011 . .

TY  - JOUR
AU  - Srajer-Gajdošik, Martina
AU  - Anđelković, Uroš
AU  - Gašo Sokač, Dajana
AU  - Pavlović, Hrvoje
AU  - Shevchuk, Olga
AU  - Martinović, Tamara
AU  - Clifton, James
AU  - Josić, Djuro
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6918
AB  - A comprehensive proteomic analysis of food borne pathogens after treatment with disinfectants based on ammonium salts of pyridinium oxime was performed. Changes in proteomes of the Gram-positive bacterium Bacillus subtilis and the Gram-negative one, Escherichia coli, were evaluated. Up and down-regulated proteins in these bacteria after growth under the inhibition with four different disinfectants based on chloride and bromide salts of pyridinium oxime were identified and their cellular localizations and functions were determined by gene ontology searching. Proteome changes presented here demonstrate different mechanisms of action of these disinfectants. In the Gram-positive food pathogen Bacillus subtilis, the inhibitory substances seem to act mainly at the cell surface and cause significant alterations of membrane and cell surface proteins. On the other hand, intracellular proteins were more affected in the Gram-negative pathogen Escherichia coli. This research is a contribution to the investigation of the virulence and pathogenicity of food borne bacteria and their survival under stress conditions, and can also lead the way for further development of new inhibitors of microbial growth and studies of mechanism of their actions.
PB  - Elsevier
T2  - Food Research International
T1  - Proteomic analysis of food borne pathogens following the mode of action of the disinfectants based on pyridoxal oxime derivatives
VL  - 99
SP  - 560
EP  - 570
DO  - 10.1016/j.foodres.2017.06.016
ER  - 

@article{
author = "Srajer-Gajdošik, Martina and Anđelković, Uroš and Gašo Sokač, Dajana and Pavlović, Hrvoje and Shevchuk, Olga and Martinović, Tamara and Clifton, James and Josić, Djuro",
year = "2017",
abstract = "A comprehensive proteomic analysis of food borne pathogens after treatment with disinfectants based on ammonium salts of pyridinium oxime was performed. Changes in proteomes of the Gram-positive bacterium Bacillus subtilis and the Gram-negative one, Escherichia coli, were evaluated. Up and down-regulated proteins in these bacteria after growth under the inhibition with four different disinfectants based on chloride and bromide salts of pyridinium oxime were identified and their cellular localizations and functions were determined by gene ontology searching. Proteome changes presented here demonstrate different mechanisms of action of these disinfectants. In the Gram-positive food pathogen Bacillus subtilis, the inhibitory substances seem to act mainly at the cell surface and cause significant alterations of membrane and cell surface proteins. On the other hand, intracellular proteins were more affected in the Gram-negative pathogen Escherichia coli. This research is a contribution to the investigation of the virulence and pathogenicity of food borne bacteria and their survival under stress conditions, and can also lead the way for further development of new inhibitors of microbial growth and studies of mechanism of their actions.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Proteomic analysis of food borne pathogens following the mode of action of the disinfectants based on pyridoxal oxime derivatives",
volume = "99",
pages = "560-570",
doi = "10.1016/j.foodres.2017.06.016"
}

Srajer-Gajdošik, M., Anđelković, U., Gašo Sokač, D., Pavlović, H., Shevchuk, O., Martinović, T., Clifton, J.,& Josić, D.. (2017). Proteomic analysis of food borne pathogens following the mode of action of the disinfectants based on pyridoxal oxime derivatives. in Food Research International
Elsevier., 99, 560-570.
https://doi.org/10.1016/j.foodres.2017.06.016

Srajer-Gajdošik M, Anđelković U, Gašo Sokač D, Pavlović H, Shevchuk O, Martinović T, Clifton J, Josić D. Proteomic analysis of food borne pathogens following the mode of action of the disinfectants based on pyridoxal oxime derivatives. in Food Research International. 2017;99:560-570.
doi:10.1016/j.foodres.2017.06.016 .

Srajer-Gajdošik, Martina, Anđelković, Uroš, Gašo Sokač, Dajana, Pavlović, Hrvoje, Shevchuk, Olga, Martinović, Tamara, Clifton, James, Josić, Djuro, "Proteomic analysis of food borne pathogens following the mode of action of the disinfectants based on pyridoxal oxime derivatives" in Food Research International, 99 (2017):560-570,
https://doi.org/10.1016/j.foodres.2017.06.016 . .

TY  - JOUR
AU  - Srajer-Gajdošik, Martina
AU  - Anđelković, Uroš
AU  - Gašo Sokač, Dajana
AU  - Pavlović, Hrvoje
AU  - Shevchuk, Olga
AU  - Martinović, Tamara
AU  - Clifton, James
AU  - Josić, Djuro
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6919
AB  - Food borne pathogens, namely the Gram-positive bacterium Bacillus subtilis and the Gram-negative bacterium Escherichia coli, were grown under the inhibition with four different disinfectants based on chloride and bromide salts of pyridinium oxime. Bacterial samples were subjected to the sequential extraction of proteins and the in-solution tryptic digestion of obtained extracts was performed prior to the identification of proteins with LC-ESI-MS/MS. Proteomic analysis identified up- and down-regulated proteins in these bacteria after treatment with each compound. The tables with differently expressed proteins are presented with this article.
PB  - Elsevier
T2  - Data in brief
T1  - Data set of proteomic analysis of food borne pathogens after treatment with the disinfectants based on pyridoxal oxime derivatives
VL  - 15
SP  - 738
EP  - 741
DO  - 10.1016/j.dib.2017.09.060
ER  - 

@article{
author = "Srajer-Gajdošik, Martina and Anđelković, Uroš and Gašo Sokač, Dajana and Pavlović, Hrvoje and Shevchuk, Olga and Martinović, Tamara and Clifton, James and Josić, Djuro",
year = "2017",
abstract = "Food borne pathogens, namely the Gram-positive bacterium Bacillus subtilis and the Gram-negative bacterium Escherichia coli, were grown under the inhibition with four different disinfectants based on chloride and bromide salts of pyridinium oxime. Bacterial samples were subjected to the sequential extraction of proteins and the in-solution tryptic digestion of obtained extracts was performed prior to the identification of proteins with LC-ESI-MS/MS. Proteomic analysis identified up- and down-regulated proteins in these bacteria after treatment with each compound. The tables with differently expressed proteins are presented with this article.",
publisher = "Elsevier",
journal = "Data in brief",
title = "Data set of proteomic analysis of food borne pathogens after treatment with the disinfectants based on pyridoxal oxime derivatives",
volume = "15",
pages = "738-741",
doi = "10.1016/j.dib.2017.09.060"
}

Srajer-Gajdošik, M., Anđelković, U., Gašo Sokač, D., Pavlović, H., Shevchuk, O., Martinović, T., Clifton, J.,& Josić, D.. (2017). Data set of proteomic analysis of food borne pathogens after treatment with the disinfectants based on pyridoxal oxime derivatives. in Data in brief
Elsevier., 15, 738-741.
https://doi.org/10.1016/j.dib.2017.09.060

Srajer-Gajdošik M, Anđelković U, Gašo Sokač D, Pavlović H, Shevchuk O, Martinović T, Clifton J, Josić D. Data set of proteomic analysis of food borne pathogens after treatment with the disinfectants based on pyridoxal oxime derivatives. in Data in brief. 2017;15:738-741.
doi:10.1016/j.dib.2017.09.060 .

Srajer-Gajdošik, Martina, Anđelković, Uroš, Gašo Sokač, Dajana, Pavlović, Hrvoje, Shevchuk, Olga, Martinović, Tamara, Clifton, James, Josić, Djuro, "Data set of proteomic analysis of food borne pathogens after treatment with the disinfectants based on pyridoxal oxime derivatives" in Data in brief, 15 (2017):738-741,
https://doi.org/10.1016/j.dib.2017.09.060 . .

TY  - JOUR
AU  - Martinović, Tamara
AU  - Anđelković, Uroš
AU  - Klobučar, Marko
AU  - Černigoj, Urh
AU  - Vidič, Jana
AU  - Lučić, Marina
AU  - Pavelić, Krešimir
AU  - Josić, Djuro
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6922
AB  - Posttranslational modifications of immunoglobulins have been a topic of great interest and have been repeatedly reported as a major factor in disease pathology. Cost-effective, reproducible, and high-throughput (HTP) isolation of immunoglobulins from human serum is vital for studying the changes in protein structure and the following understanding of disease development. Although there are many methods for the isolation of specific immunoglobulin classes, only a few of them are applicable for isolation of all subtypes and variants. Here, we present the development of a scheme for fast and simultaneous affinity purification of α (A), γ (G), and μ (M) immunoglobulins from human serum through affinity monolith chromatography. Affinity-based monolithic columns with immobilized protein A, G, or L were used for antibody isolation. Monolithic stationary phases have a high surface accessibility of binding sites, large flow-through channels, and can be operated at high flow rates, making them the ideal supports for HTP isolation of biopolymers. The presented method can be used for HTP screening of human serum in order to simultaneously isolate all three above-mentioned immunoglobulins and determine their concentration and changes in their glycosylation pattern as potential prognostic and diagnostic disease biomarkers.
PB  - Willey
T2  - Electrophoresis
T1  - Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum
VL  - 38
IS  - 22-23
SP  - 2909
EP  - 2913
DO  - 10.1002/elps.201700216
ER  - 

@article{
author = "Martinović, Tamara and Anđelković, Uroš and Klobučar, Marko and Černigoj, Urh and Vidič, Jana and Lučić, Marina and Pavelić, Krešimir and Josić, Djuro",
year = "2017",
abstract = "Posttranslational modifications of immunoglobulins have been a topic of great interest and have been repeatedly reported as a major factor in disease pathology. Cost-effective, reproducible, and high-throughput (HTP) isolation of immunoglobulins from human serum is vital for studying the changes in protein structure and the following understanding of disease development. Although there are many methods for the isolation of specific immunoglobulin classes, only a few of them are applicable for isolation of all subtypes and variants. Here, we present the development of a scheme for fast and simultaneous affinity purification of α (A), γ (G), and μ (M) immunoglobulins from human serum through affinity monolith chromatography. Affinity-based monolithic columns with immobilized protein A, G, or L were used for antibody isolation. Monolithic stationary phases have a high surface accessibility of binding sites, large flow-through channels, and can be operated at high flow rates, making them the ideal supports for HTP isolation of biopolymers. The presented method can be used for HTP screening of human serum in order to simultaneously isolate all three above-mentioned immunoglobulins and determine their concentration and changes in their glycosylation pattern as potential prognostic and diagnostic disease biomarkers.",
publisher = "Willey",
journal = "Electrophoresis",
title = "Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum",
volume = "38",
number = "22-23",
pages = "2909-2913",
doi = "10.1002/elps.201700216"
}

Martinović, T., Anđelković, U., Klobučar, M., Černigoj, U., Vidič, J., Lučić, M., Pavelić, K.,& Josić, D.. (2017). Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum. in Electrophoresis
Willey., 38(22-23), 2909-2913.
https://doi.org/10.1002/elps.201700216

Martinović T, Anđelković U, Klobučar M, Černigoj U, Vidič J, Lučić M, Pavelić K, Josić D. Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum. in Electrophoresis. 2017;38(22-23):2909-2913.
doi:10.1002/elps.201700216 .

Martinović, Tamara, Anđelković, Uroš, Klobučar, Marko, Černigoj, Urh, Vidič, Jana, Lučić, Marina, Pavelić, Krešimir, Josić, Djuro, "Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum" in Electrophoresis, 38, no. 22-23 (2017):2909-2913,
https://doi.org/10.1002/elps.201700216 . .

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Šrajer Gajdošik, Martina
AU  - Gašo-Sokač, Dajana
AU  - Martinović, Tamara
AU  - Josić, Đuro
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2156
AB  - The power of foodomics as a discipline that is now broadly used for quality assurance of food products and adulteration identifi cation, as well as for determining the safety of food, is presented. Concerning sample preparation and application, maintenance of highly sophisticated instruments for both high-performance and high-throughput techniques, and analysis and data interpretation, special att ention has to be paid to the development of skilled analysts. The obtained data shall be integrated under a strong bioinformatics environment. Modern mass spectrometry is an extremely powerful analytical tool since it can provide direct qualitative and quantitative information about a molecule of interest from only a minute amount of sample. Quality of this information is infl uenced by the sample preparation procedure, the type of mass spectrometer used and the analyst's skills. Technical advances are bringing new instruments of increased sensitivity, resolution and speed to the market. Other methods presented here give additional information and can be used as complementary tools to mass spectrometry or for validation of obtained results. Genomics and transcriptomics, as well as affi nity-based methods, still have a broad use in food analysis. Serious drawbacks of some of them, especially the affi nity-based methods, are the cross-reactivity between similar molecules and the infl uence of complex food matrices. However, these techniques can be used for pre-screening in order to reduce the large number of samples. Great progress has been made in the application of bioinformatics in foodomics. These developments enabled processing of large amounts of generated data for both identifi cation and quantifi cation, and for corresponding modeling.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Foodomics and Food Safety: Where We Are
VL  - 55
IS  - 3
SP  - 290
EP  - 307
DO  - 10.17113/ftb.55.03.17.5044
ER  - 

@article{
author = "Anđelković, Uroš and Šrajer Gajdošik, Martina and Gašo-Sokač, Dajana and Martinović, Tamara and Josić, Đuro",
year = "2017",
abstract = "The power of foodomics as a discipline that is now broadly used for quality assurance of food products and adulteration identifi cation, as well as for determining the safety of food, is presented. Concerning sample preparation and application, maintenance of highly sophisticated instruments for both high-performance and high-throughput techniques, and analysis and data interpretation, special att ention has to be paid to the development of skilled analysts. The obtained data shall be integrated under a strong bioinformatics environment. Modern mass spectrometry is an extremely powerful analytical tool since it can provide direct qualitative and quantitative information about a molecule of interest from only a minute amount of sample. Quality of this information is infl uenced by the sample preparation procedure, the type of mass spectrometer used and the analyst's skills. Technical advances are bringing new instruments of increased sensitivity, resolution and speed to the market. Other methods presented here give additional information and can be used as complementary tools to mass spectrometry or for validation of obtained results. Genomics and transcriptomics, as well as affi nity-based methods, still have a broad use in food analysis. Serious drawbacks of some of them, especially the affi nity-based methods, are the cross-reactivity between similar molecules and the infl uence of complex food matrices. However, these techniques can be used for pre-screening in order to reduce the large number of samples. Great progress has been made in the application of bioinformatics in foodomics. These developments enabled processing of large amounts of generated data for both identifi cation and quantifi cation, and for corresponding modeling.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Foodomics and Food Safety: Where We Are",
volume = "55",
number = "3",
pages = "290-307",
doi = "10.17113/ftb.55.03.17.5044"
}

Anđelković, U., Šrajer Gajdošik, M., Gašo-Sokač, D., Martinović, T.,& Josić, Đ.. (2017). Foodomics and Food Safety: Where We Are. in Food Technology and Biotechnology
University of Zagreb., 55(3), 290-307.
https://doi.org/10.17113/ftb.55.03.17.5044

Anđelković U, Šrajer Gajdošik M, Gašo-Sokač D, Martinović T, Josić Đ. Foodomics and Food Safety: Where We Are. in Food Technology and Biotechnology. 2017;55(3):290-307.
doi:10.17113/ftb.55.03.17.5044 .

Anđelković, Uroš, Šrajer Gajdošik, Martina, Gašo-Sokač, Dajana, Martinović, Tamara, Josić, Đuro, "Foodomics and Food Safety: Where We Are" in Food Technology and Biotechnology, 55, no. 3 (2017):290-307,
https://doi.org/10.17113/ftb.55.03.17.5044 . .

TY  - CHAP
AU  - Anđelković, Uroš
AU  - Giacometti, Jasminka
AU  - Josić, Đuro
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4233
AB  - Since the introduction of suitable, highly porous supports and later nonporous and monolithic supports, chromatography has been an indispensibleindispensable method for protein separations on the analytical and preparative scales. The most frequently used methods for protein chromatography are ion exchange, reversed- phase, hydrophobic hydrophobic-interaction chromatography, chromatography on hydroxyapatite and different types of affinity, and pseudo-affinity chromatography. Because of the use of organic solvents during the separation in reversed-phase chromatography, denaturation and loss of biological activity frequently occurs, and this method is less suitable for the separation of biologically active, therapeutic proteins. Chromatography on monolithic supports and chromatography in the displacement mode offer additional opportunities for fast, highly effective separation of proteins on both the analytical and preparative scales.
PB  - Elsevier
T2  - Liquid Chromatography: Applications
T1  - Protein and Peptide Separations
VL  - 2
SP  - 107
EP  - 157
DO  - 10.1016/B978-0-12-805392-8.00005-0
ER  - 

@inbook{
author = "Anđelković, Uroš and Giacometti, Jasminka and Josić, Đuro",
year = "2017",
abstract = "Since the introduction of suitable, highly porous supports and later nonporous and monolithic supports, chromatography has been an indispensibleindispensable method for protein separations on the analytical and preparative scales. The most frequently used methods for protein chromatography are ion exchange, reversed- phase, hydrophobic hydrophobic-interaction chromatography, chromatography on hydroxyapatite and different types of affinity, and pseudo-affinity chromatography. Because of the use of organic solvents during the separation in reversed-phase chromatography, denaturation and loss of biological activity frequently occurs, and this method is less suitable for the separation of biologically active, therapeutic proteins. Chromatography on monolithic supports and chromatography in the displacement mode offer additional opportunities for fast, highly effective separation of proteins on both the analytical and preparative scales.",
publisher = "Elsevier",
journal = "Liquid Chromatography: Applications",
booktitle = "Protein and Peptide Separations",
volume = "2",
pages = "107-157",
doi = "10.1016/B978-0-12-805392-8.00005-0"
}

Anđelković, U., Giacometti, J.,& Josić, Đ.. (2017). Protein and Peptide Separations. in Liquid Chromatography: Applications
Elsevier., 2, 107-157.
https://doi.org/10.1016/B978-0-12-805392-8.00005-0

Anđelković U, Giacometti J, Josić Đ. Protein and Peptide Separations. in Liquid Chromatography: Applications. 2017;2:107-157.
doi:10.1016/B978-0-12-805392-8.00005-0 .

Anđelković, Uroš, Giacometti, Jasminka, Josić, Đuro, "Protein and Peptide Separations" in Liquid Chromatography: Applications, 2 (2017):107-157,
https://doi.org/10.1016/B978-0-12-805392-8.00005-0 . .

TY  - CHAP
AU  - Rešetar, Dina
AU  - Martinović, Tamara
AU  - Kraljević Pavelić, Sandra
AU  - Anđelković, Uroš
AU  - Josić, Đuro
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4230
AB  - Proteomics and peptidomics are key foodomic techniques that are indispensable for monitoring of pathogen contamination in foods during their production, storage and transportation, up to their consumption. The thick peptidoglycan layer that stains during the standard Gram‐stain test, and that ensures the exposure of purple Gram‐positive bacterial cells under the microscope, actually protects Gram‐positive bacteria from environmental factors and provides certain survival advantages. The World Health Organization (WHO) estimates that Gram‐negative Campylobacter bacteria, together with norovirus, are the most frequent causes of diarrhoeal disease associated with consumption of unsafe food. Different food‐borne bacterial toxins cause irreversible modifications of the host cellular targets, resulting in extensive losses in their function. Different PCR/qPCR‐based techniques, second and third generation sequencing techniques and other nucleic acid‐based methods are powerful techniques for bacterial detection. This chapter provides an overview on what contemporary proteomic/peptidomic tools are providing in detection and quantification of bacteria in food samples.
PB  - John Wiley & Sons, Ltd
T2  - Advances in Food Diagnostics
T1  - Proteomics and Peptidomics as Tools for Detection of Food Contamination by Bacteria
SP  - 97
EP  - 137
DO  - 10.1002/9781119105916.ch4
ER  - 

@inbook{
author = "Rešetar, Dina and Martinović, Tamara and Kraljević Pavelić, Sandra and Anđelković, Uroš and Josić, Đuro",
year = "2017",
abstract = "Proteomics and peptidomics are key foodomic techniques that are indispensable for monitoring of pathogen contamination in foods during their production, storage and transportation, up to their consumption. The thick peptidoglycan layer that stains during the standard Gram‐stain test, and that ensures the exposure of purple Gram‐positive bacterial cells under the microscope, actually protects Gram‐positive bacteria from environmental factors and provides certain survival advantages. The World Health Organization (WHO) estimates that Gram‐negative Campylobacter bacteria, together with norovirus, are the most frequent causes of diarrhoeal disease associated with consumption of unsafe food. Different food‐borne bacterial toxins cause irreversible modifications of the host cellular targets, resulting in extensive losses in their function. Different PCR/qPCR‐based techniques, second and third generation sequencing techniques and other nucleic acid‐based methods are powerful techniques for bacterial detection. This chapter provides an overview on what contemporary proteomic/peptidomic tools are providing in detection and quantification of bacteria in food samples.",
publisher = "John Wiley & Sons, Ltd",
journal = "Advances in Food Diagnostics",
booktitle = "Proteomics and Peptidomics as Tools for Detection of Food Contamination by Bacteria",
pages = "97-137",
doi = "10.1002/9781119105916.ch4"
}

Rešetar, D., Martinović, T., Kraljević Pavelić, S., Anđelković, U.,& Josić, Đ.. (2017). Proteomics and Peptidomics as Tools for Detection of Food Contamination by Bacteria. in Advances in Food Diagnostics
John Wiley & Sons, Ltd., 97-137.
https://doi.org/10.1002/9781119105916.ch4

Rešetar D, Martinović T, Kraljević Pavelić S, Anđelković U, Josić Đ. Proteomics and Peptidomics as Tools for Detection of Food Contamination by Bacteria. in Advances in Food Diagnostics. 2017;:97-137.
doi:10.1002/9781119105916.ch4 .

Rešetar, Dina, Martinović, Tamara, Kraljević Pavelić, Sandra, Anđelković, Uroš, Josić, Đuro, "Proteomics and Peptidomics as Tools for Detection of Food Contamination by Bacteria" in Advances in Food Diagnostics (2017):97-137,
https://doi.org/10.1002/9781119105916.ch4 . .

TY  - JOUR
AU  - Breen, L.D.
AU  - Pučić-Baković, M
AU  - Vučković, F.
AU  - Reiding, K.
AU  - Trbojević-Akmačić, I.
AU  - Srajer-Gajdošik, M.
AU  - Cook, M.I.
AU  - Lopez, M.J.
AU  - Wuhrer, M.
AU  - Camara, L.M.
AU  - Anđelković, Uroš
AU  - Dupuy, D.E.
AU  - Josić, Djuro
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6914
AB  - Background
Image-guided tumor ablation is a technique whereby needle-like applicators are placed directly into solid tumors under guidance typically with computed tomography or ultrasound. Changes in IgG and IgM antibody glycosylation were studied during ablation-induced immune response to cancer, and the use of glycosylation as a biomarker for diagnosis, prognosis and disease treatment was examined.

Methods
Plasma from 27 tumor patients was collected immediately before, after and for 6 months following ablation. IgG and IgM antibodies were isolated by use high-throughput chromatography, and analyzed by hydrophilic liquid chromatography. Thorough identification of glycan structures in each chromatography peak was performed by nano-liquid chromatography electrospray ionization mass spectrometry.

Results
Although antibody glycosylation was found to vary with cancer type, discernable patterns of change based on the successful treatment of tumors by ablation were not identified. One patient with renal clear cell carcinoma and poor disease outcome had unexpectedly high amount of oligomannose IgG glycans during the whole period of monitoring. In contrast, IgM antibodies did not follow the same pattern.

Conclusions
These findings suggest that glycosylation patterns are indicative of an immune system that is unable to prevent different types of cancer, rather than products of the immunostimulatory response to the ablation of tumor itself. Analyses of the outcome effect suggested that IgG glycosylation and IgM glycosylation are not associated with tumor ablation.

General significance
Present work opens a new way for parallel determination of glycosylation changes of both IgG and IgM antibodies by use of high-throughput methods, and their future use as biomarkers for disease diagnosis and prognosis. This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
PB  - Elsevier
T2  - Biochimica et Biophysica Acta: General Subjects
T1  - IgG and IgM glycosylation patterns in patients undergoing image-guided tumor ablation
VL  - 1860
IS  - 8
SP  - 1786
EP  - 1794
DO  - 10.1016/j.bbagen.2016.01.011
ER  - 

@article{
author = "Breen, L.D. and Pučić-Baković, M and Vučković, F. and Reiding, K. and Trbojević-Akmačić, I. and Srajer-Gajdošik, M. and Cook, M.I. and Lopez, M.J. and Wuhrer, M. and Camara, L.M. and Anđelković, Uroš and Dupuy, D.E. and Josić, Djuro",
year = "2016",
abstract = "Background
Image-guided tumor ablation is a technique whereby needle-like applicators are placed directly into solid tumors under guidance typically with computed tomography or ultrasound. Changes in IgG and IgM antibody glycosylation were studied during ablation-induced immune response to cancer, and the use of glycosylation as a biomarker for diagnosis, prognosis and disease treatment was examined.

Methods
Plasma from 27 tumor patients was collected immediately before, after and for 6 months following ablation. IgG and IgM antibodies were isolated by use high-throughput chromatography, and analyzed by hydrophilic liquid chromatography. Thorough identification of glycan structures in each chromatography peak was performed by nano-liquid chromatography electrospray ionization mass spectrometry.

Results
Although antibody glycosylation was found to vary with cancer type, discernable patterns of change based on the successful treatment of tumors by ablation were not identified. One patient with renal clear cell carcinoma and poor disease outcome had unexpectedly high amount of oligomannose IgG glycans during the whole period of monitoring. In contrast, IgM antibodies did not follow the same pattern.

Conclusions
These findings suggest that glycosylation patterns are indicative of an immune system that is unable to prevent different types of cancer, rather than products of the immunostimulatory response to the ablation of tumor itself. Analyses of the outcome effect suggested that IgG glycosylation and IgM glycosylation are not associated with tumor ablation.

General significance
Present work opens a new way for parallel determination of glycosylation changes of both IgG and IgM antibodies by use of high-throughput methods, and their future use as biomarkers for disease diagnosis and prognosis. This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.",
publisher = "Elsevier",
journal = "Biochimica et Biophysica Acta: General Subjects",
title = "IgG and IgM glycosylation patterns in patients undergoing image-guided tumor ablation",
volume = "1860",
number = "8",
pages = "1786-1794",
doi = "10.1016/j.bbagen.2016.01.011"
}

Breen, L.D., Pučić-Baković, M., Vučković, F., Reiding, K., Trbojević-Akmačić, I., Srajer-Gajdošik, M., Cook, M.I., Lopez, M.J., Wuhrer, M., Camara, L.M., Anđelković, U., Dupuy, D.E.,& Josić, D.. (2016). IgG and IgM glycosylation patterns in patients undergoing image-guided tumor ablation. in Biochimica et Biophysica Acta: General Subjects
Elsevier., 1860(8), 1786-1794.
https://doi.org/10.1016/j.bbagen.2016.01.011

Breen L, Pučić-Baković M, Vučković F, Reiding K, Trbojević-Akmačić I, Srajer-Gajdošik M, Cook M, Lopez M, Wuhrer M, Camara L, Anđelković U, Dupuy D, Josić D. IgG and IgM glycosylation patterns in patients undergoing image-guided tumor ablation. in Biochimica et Biophysica Acta: General Subjects. 2016;1860(8):1786-1794.
doi:10.1016/j.bbagen.2016.01.011 .

Breen, L.D., Pučić-Baković, M, Vučković, F., Reiding, K., Trbojević-Akmačić, I., Srajer-Gajdošik, M., Cook, M.I., Lopez, M.J., Wuhrer, M., Camara, L.M., Anđelković, Uroš, Dupuy, D.E., Josić, Djuro, "IgG and IgM glycosylation patterns in patients undergoing image-guided tumor ablation" in Biochimica et Biophysica Acta: General Subjects, 1860, no. 8 (2016):1786-1794,
https://doi.org/10.1016/j.bbagen.2016.01.011 . .

TY  - JOUR
AU  - Martinović, Tamara
AU  - Anđelković, Uroš
AU  - Srajer-Gajdošik, Martina
AU  - Rešetar, Dina
AU  - Josić, Djuro
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6915
AB  - Foodborne pathogens, mostly bacteria and fungi, but also some viruses, prions and protozoa, contaminate food during production and processing, but also during storage and transport before consuming. During their growth these microorganisms can secrete different components, including toxins, into the extracellular environment. Other harmful substances can be also liberated and can contaminate food after disintegration of food pathogens. Some bacterial and fungal toxins can be resistant to inactivation, and can survive harsh treatment during food processing. Many of these molecules are involved in cellular processes and can indicate different mechanisms of pathogenesis of foodborne organisms. More knowledge about food contaminants can also help understand their inactivation. In the present review the use of proteomics, peptidomics and metabolomics, in addition to other foodomic methods for the detection of foodborne pathogenic fungi and bacteria, is overviewed. Furthermore, it is discussed how these techniques can be used for discovering biomarkers for pathogenicity of foodborne pathogens, determining the mechanisms by which they act, and studying their resistance upon inactivation in food of animal and plant origin.

Biological significance
Comprehensive and comparative view into the genome and proteome of foodborne pathogens of bacterial or fungal origin and foodomic, mostly proteomic, peptidomic and metabolomic investigation of their toxin production and their mechanism of action is necessary in order to get further information about their virulence, pathogenicity and survival under stress conditions. Furthermore, these data pave the way for identification of biomarkers to trace sources of contamination with food-borne microorganisms and their endo- and exotoxins in order to ensure food safety and prevent the outbreak of food-borne diseases. Therefore, detection of pathogens and their toxins during production, transport and before consume of food produce, as well as protection against food spoilage is a task of great social, economic and public health importance.
PB  - Elsevier
T2  - Journal of Proteomics
T1  - Foodborne pathogens and their toxins
VL  - 147
SP  - 226
EP  - 235
DO  - 10.1016/j.jprot.2016.04.029
ER  - 

@article{
author = "Martinović, Tamara and Anđelković, Uroš and Srajer-Gajdošik, Martina and Rešetar, Dina and Josić, Djuro",
year = "2016",
abstract = "Foodborne pathogens, mostly bacteria and fungi, but also some viruses, prions and protozoa, contaminate food during production and processing, but also during storage and transport before consuming. During their growth these microorganisms can secrete different components, including toxins, into the extracellular environment. Other harmful substances can be also liberated and can contaminate food after disintegration of food pathogens. Some bacterial and fungal toxins can be resistant to inactivation, and can survive harsh treatment during food processing. Many of these molecules are involved in cellular processes and can indicate different mechanisms of pathogenesis of foodborne organisms. More knowledge about food contaminants can also help understand their inactivation. In the present review the use of proteomics, peptidomics and metabolomics, in addition to other foodomic methods for the detection of foodborne pathogenic fungi and bacteria, is overviewed. Furthermore, it is discussed how these techniques can be used for discovering biomarkers for pathogenicity of foodborne pathogens, determining the mechanisms by which they act, and studying their resistance upon inactivation in food of animal and plant origin.

Biological significance
Comprehensive and comparative view into the genome and proteome of foodborne pathogens of bacterial or fungal origin and foodomic, mostly proteomic, peptidomic and metabolomic investigation of their toxin production and their mechanism of action is necessary in order to get further information about their virulence, pathogenicity and survival under stress conditions. Furthermore, these data pave the way for identification of biomarkers to trace sources of contamination with food-borne microorganisms and their endo- and exotoxins in order to ensure food safety and prevent the outbreak of food-borne diseases. Therefore, detection of pathogens and their toxins during production, transport and before consume of food produce, as well as protection against food spoilage is a task of great social, economic and public health importance.",
publisher = "Elsevier",
journal = "Journal of Proteomics",
title = "Foodborne pathogens and their toxins",
volume = "147",
pages = "226-235",
doi = "10.1016/j.jprot.2016.04.029"
}

Martinović, T., Anđelković, U., Srajer-Gajdošik, M., Rešetar, D.,& Josić, D.. (2016). Foodborne pathogens and their toxins. in Journal of Proteomics
Elsevier., 147, 226-235.
https://doi.org/10.1016/j.jprot.2016.04.029

Martinović T, Anđelković U, Srajer-Gajdošik M, Rešetar D, Josić D. Foodborne pathogens and their toxins. in Journal of Proteomics. 2016;147:226-235.
doi:10.1016/j.jprot.2016.04.029 .

Martinović, Tamara, Anđelković, Uroš, Srajer-Gajdošik, Martina, Rešetar, Dina, Josić, Djuro, "Foodborne pathogens and their toxins" in Journal of Proteomics, 147 (2016):226-235,
https://doi.org/10.1016/j.jprot.2016.04.029 . .

TY  - JOUR
AU  - Malatesti, Nela
AU  - Harej, Anja
AU  - Kraljević Pavelić, Sandra
AU  - Lončarić, M.
AU  - Zorc, H.
AU  - Wittine, Karlo
AU  - Anđelković, Uroš
AU  - Josić, Djuro
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6916
AB  - Photodynamic therapy (PDT) is a treatment that aims to kill cancer cells by reactive oxygen species, mainly singlet oxygen, produced through light activation of a photosensitiser (PS). Amongst photosensitisers that attracted the most attention in the last decade are cationic and amphiphilic molecules based on porphyrin, chlorin and phthalocyanine structures. Our aim was to join this search for more optimal balance of the lipophilic and hydrophilic moieties in a PS. A new amphiphilic porphyrin, 5-(4-octadecanamidophenyl)-10,15,20-tris(N-methylpyridinium-3-yl)porphyrin trichloride (5) was synthesised and characterised by 1H NMR, UV–vis and fluorescence spectroscopy, and by MALDI-TOF/TOF spectrometry. In vitro photodynamic activity of 5 was evaluated on HeLa cell lines and compared to the activity of the hydrophilic 5-(4-acetamidophenyl)-10,15,20-tris(N-methylpyridinium-3-yl)porphyrin trichloride (7). Low fluence rate (2 mW cm−2) of red light (643 nm) was used for the activation, and both porphyrins showed a drug dose-response as well as a light dose-response relationship, but the amphiphilic porphyrin was presented with significantly lower IC50 values. The obtained IC50 values for 5 were 1.4 μM at 15 min irradiation time and 0.7 μM when the time of irradiation was 30 min, while for 7 these values were 37 and 6 times higher, respectively. These results confirm the importance of the lipophilic component in a PS and show a potential for 5 to be used as a PS in PDT applications.
PB  - Elsevier
T2  - Photodiagnosis and Photodynamic Therapy
T1  - Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4-octadecanamidophenyl)-10,15,20-tris(N-methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate
VL  - 15
SP  - 115
EP  - 126
DO  - 10.1016/j.pdpdt.2016.07.003
ER  - 

@article{
author = "Malatesti, Nela and Harej, Anja and Kraljević Pavelić, Sandra and Lončarić, M. and Zorc, H. and Wittine, Karlo and Anđelković, Uroš and Josić, Djuro",
year = "2016",
abstract = "Photodynamic therapy (PDT) is a treatment that aims to kill cancer cells by reactive oxygen species, mainly singlet oxygen, produced through light activation of a photosensitiser (PS). Amongst photosensitisers that attracted the most attention in the last decade are cationic and amphiphilic molecules based on porphyrin, chlorin and phthalocyanine structures. Our aim was to join this search for more optimal balance of the lipophilic and hydrophilic moieties in a PS. A new amphiphilic porphyrin, 5-(4-octadecanamidophenyl)-10,15,20-tris(N-methylpyridinium-3-yl)porphyrin trichloride (5) was synthesised and characterised by 1H NMR, UV–vis and fluorescence spectroscopy, and by MALDI-TOF/TOF spectrometry. In vitro photodynamic activity of 5 was evaluated on HeLa cell lines and compared to the activity of the hydrophilic 5-(4-acetamidophenyl)-10,15,20-tris(N-methylpyridinium-3-yl)porphyrin trichloride (7). Low fluence rate (2 mW cm−2) of red light (643 nm) was used for the activation, and both porphyrins showed a drug dose-response as well as a light dose-response relationship, but the amphiphilic porphyrin was presented with significantly lower IC50 values. The obtained IC50 values for 5 were 1.4 μM at 15 min irradiation time and 0.7 μM when the time of irradiation was 30 min, while for 7 these values were 37 and 6 times higher, respectively. These results confirm the importance of the lipophilic component in a PS and show a potential for 5 to be used as a PS in PDT applications.",
publisher = "Elsevier",
journal = "Photodiagnosis and Photodynamic Therapy",
title = "Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4-octadecanamidophenyl)-10,15,20-tris(N-methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate",
volume = "15",
pages = "115-126",
doi = "10.1016/j.pdpdt.2016.07.003"
}

Malatesti, N., Harej, A., Kraljević Pavelić, S., Lončarić, M., Zorc, H., Wittine, K., Anđelković, U.,& Josić, D.. (2016). Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4-octadecanamidophenyl)-10,15,20-tris(N-methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate. in Photodiagnosis and Photodynamic Therapy
Elsevier., 15, 115-126.
https://doi.org/10.1016/j.pdpdt.2016.07.003

Malatesti N, Harej A, Kraljević Pavelić S, Lončarić M, Zorc H, Wittine K, Anđelković U, Josić D. Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4-octadecanamidophenyl)-10,15,20-tris(N-methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate. in Photodiagnosis and Photodynamic Therapy. 2016;15:115-126.
doi:10.1016/j.pdpdt.2016.07.003 .

Malatesti, Nela, Harej, Anja, Kraljević Pavelić, Sandra, Lončarić, M., Zorc, H., Wittine, Karlo, Anđelković, Uroš, Josić, Djuro, "Synthesis, characterisation and in vitro investigation of photodynamic activity of 5-(4-octadecanamidophenyl)-10,15,20-tris(N-methylpyridinium-3-yl)porphyrin trichloride on HeLa cells using low light fluence rate" in Photodiagnosis and Photodynamic Therapy, 15 (2016):115-126,
https://doi.org/10.1016/j.pdpdt.2016.07.003 . .

TY  - CHAP
AU  - Josić, Đuro
AU  - Anđelković, Uroš
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4232
AB  - The consequences of the differences in the genome of each human individual are the variation in expressed protein isoforms, as well as the changes of the level and timing of protein expression and of the spatial distribution of expressed proteins. These differences are defined as differences in human proteome. The consequence of the changes of individual proteome is the difference in protein–protein interactions (interactome). The network of interactions between proteins underlines every single process in a living organism and makes it happen. This complex network is kept in more or less optimal homeostasis that is unique for each organism. Thus, the capacity of the interactome to overcome disease condition differs among individuals, e.g., to compensate over or down regulations of different processes that are induced, e.g., by mutations. Recent technological advances in high throughput proteomic techniques enabled fast and deep analysis of human proteome and also some understanding of the complex mechanism of protein–protein interactions. However, the human proteome analysis is still not complete, and further improvements in both analytical techniques and accompanying bioinformatics tools are necessary. In this chapter, current state of application of different proteomic approaches for personalized patient proteome profiling and search for diagnostic and prognostic disease biomarkers are presented and the contribution of proteome analysis to personalized approach in most frequent diseases in developed Western World, namely cancer, cardiovascular, urological and neurodegenerative diseases, diabetes mellitus and allergies, has been reviewed.
PB  - Springer
T2  - Personalized medicine: A New Medical and Social Challenge
T1  - The Role of Proteomics in Personalized Medicine
VL  - 2
SP  - 179
EP  - 218
DO  - 10.1007/978-3-319-39349-0_9
ER  - 

@inbook{
author = "Josić, Đuro and Anđelković, Uroš",
year = "2016",
abstract = "The consequences of the differences in the genome of each human individual are the variation in expressed protein isoforms, as well as the changes of the level and timing of protein expression and of the spatial distribution of expressed proteins. These differences are defined as differences in human proteome. The consequence of the changes of individual proteome is the difference in protein–protein interactions (interactome). The network of interactions between proteins underlines every single process in a living organism and makes it happen. This complex network is kept in more or less optimal homeostasis that is unique for each organism. Thus, the capacity of the interactome to overcome disease condition differs among individuals, e.g., to compensate over or down regulations of different processes that are induced, e.g., by mutations. Recent technological advances in high throughput proteomic techniques enabled fast and deep analysis of human proteome and also some understanding of the complex mechanism of protein–protein interactions. However, the human proteome analysis is still not complete, and further improvements in both analytical techniques and accompanying bioinformatics tools are necessary. In this chapter, current state of application of different proteomic approaches for personalized patient proteome profiling and search for diagnostic and prognostic disease biomarkers are presented and the contribution of proteome analysis to personalized approach in most frequent diseases in developed Western World, namely cancer, cardiovascular, urological and neurodegenerative diseases, diabetes mellitus and allergies, has been reviewed.",
publisher = "Springer",
journal = "Personalized medicine: A New Medical and Social Challenge",
booktitle = "The Role of Proteomics in Personalized Medicine",
volume = "2",
pages = "179-218",
doi = "10.1007/978-3-319-39349-0_9"
}

Josić, Đ.,& Anđelković, U.. (2016). The Role of Proteomics in Personalized Medicine. in Personalized medicine: A New Medical and Social Challenge
Springer., 2, 179-218.
https://doi.org/10.1007/978-3-319-39349-0_9

Josić Đ, Anđelković U. The Role of Proteomics in Personalized Medicine. in Personalized medicine: A New Medical and Social Challenge. 2016;2:179-218.
doi:10.1007/978-3-319-39349-0_9 .

Josić, Đuro, Anđelković, Uroš, "The Role of Proteomics in Personalized Medicine" in Personalized medicine: A New Medical and Social Challenge, 2 (2016):179-218,
https://doi.org/10.1007/978-3-319-39349-0_9 . .

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Martinović, Tamara
AU  - Josić, Đuro
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4227
AB  - An overview is presented of the use of foodomics in the study of food allergies. Immunological, as well as DNA-based and mass spectrometry-based methods are the most frequently used techniques for determination of food allergens. Some immunological and DNA-based methods are suitable because of their high-throughput detection and characterization. However, these methods also bring the risk of biases. Allergen detection and quantification using mass spectrometry based foodomic techniques brings an important advantage over antibody and nucleic acid based techniques, especially regarding method reliability and reproducibility. Reliable and reproducible high-throughput detection and quantitative determination of allergens shall be implemented in food industry and control laboratories as a part of quality assurance and quality control during production and distribution.
PB  - Elsevier
T2  - Current Opinion in Food Science
T1  - Foodomic investigations of food allergies
VL  - 4
SP  - 92
EP  - 98
DO  - 10.1016/j.cofs.2015.06.003
ER  - 

@article{
author = "Anđelković, Uroš and Martinović, Tamara and Josić, Đuro",
year = "2015",
abstract = "An overview is presented of the use of foodomics in the study of food allergies. Immunological, as well as DNA-based and mass spectrometry-based methods are the most frequently used techniques for determination of food allergens. Some immunological and DNA-based methods are suitable because of their high-throughput detection and characterization. However, these methods also bring the risk of biases. Allergen detection and quantification using mass spectrometry based foodomic techniques brings an important advantage over antibody and nucleic acid based techniques, especially regarding method reliability and reproducibility. Reliable and reproducible high-throughput detection and quantitative determination of allergens shall be implemented in food industry and control laboratories as a part of quality assurance and quality control during production and distribution.",
publisher = "Elsevier",
journal = "Current Opinion in Food Science",
title = "Foodomic investigations of food allergies",
volume = "4",
pages = "92-98",
doi = "10.1016/j.cofs.2015.06.003"
}

Anđelković, U., Martinović, T.,& Josić, Đ.. (2015). Foodomic investigations of food allergies. in Current Opinion in Food Science
Elsevier., 4, 92-98.
https://doi.org/10.1016/j.cofs.2015.06.003

Anđelković U, Martinović T, Josić Đ. Foodomic investigations of food allergies. in Current Opinion in Food Science. 2015;4:92-98.
doi:10.1016/j.cofs.2015.06.003 .

Anđelković, Uroš, Martinović, Tamara, Josić, Đuro, "Foodomic investigations of food allergies" in Current Opinion in Food Science, 4 (2015):92-98,
https://doi.org/10.1016/j.cofs.2015.06.003 . .