TY  - JOUR
AU  - Jonović, Marko
AU  - Jugović, Branimir
AU  - Žuža, Milena
AU  - Đorđević, Verica
AU  - Milašinović, Nikola
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5239
AB  - The aim of this study was to investigate covalent immobilization of horseradish peroxidase (HRP) on magnetic nanoparticles (Mag) encapsulated in calcium alginate beads (MABs) for color degradation, combining easy and fast removal of biocatalyst from the reaction mixture due to its magnetic properties and strong binding due to surface alginate functional groups. MABs obtained by extrusion techniques were analyzed by optical microscopy, FEG-SEM and characterized regarding mechanical properties, magnetization and HRP binding. HRP with initial concentration of 10 mg/gcarrier was successfully covalently bonded on MABs (diameter ~1 mm, magnetite/alginate ratio 1:4), with protein loading of 8.9 mg/gcarrier, immobilization yield 96.9% and activity 32.8 U/g. Immobilized HRP on MABs (HRP-MABs) was then used to catalyze degradation of two anthraquinonic dyes, Acid Blue 225 (AB225) and Acid Violet 109 (AV109), as models for wastewater pollutants. HRP-MABs decolorized 77.3% and 76.1% of AV109 and AB225, respectively after 15 min under optimal conditions (0.097 mM H2O2, 200 mg of HRP-MABs (8.9 mg/gcarrier), 0.08 and 0.1 g/mg beads/dye ratio for AV109 and AB225, respectively). Biocatalyst was used for 7 repeated cycles retaining 75% and 51% of initial activity for AB225 and AV109, respectively, showing potential for use in large scale applications for colored wastewater treatment.
PB  - MDPI
T2  - Polymers
T1  - Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation
VL  - 14
IS  - 13
SP  - 2614
DO  - 10.3390/polym14132614
ER  - 

@article{
author = "Jonović, Marko and Jugović, Branimir and Žuža, Milena and Đorđević, Verica and Milašinović, Nikola and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2022",
abstract = "The aim of this study was to investigate covalent immobilization of horseradish peroxidase (HRP) on magnetic nanoparticles (Mag) encapsulated in calcium alginate beads (MABs) for color degradation, combining easy and fast removal of biocatalyst from the reaction mixture due to its magnetic properties and strong binding due to surface alginate functional groups. MABs obtained by extrusion techniques were analyzed by optical microscopy, FEG-SEM and characterized regarding mechanical properties, magnetization and HRP binding. HRP with initial concentration of 10 mg/gcarrier was successfully covalently bonded on MABs (diameter ~1 mm, magnetite/alginate ratio 1:4), with protein loading of 8.9 mg/gcarrier, immobilization yield 96.9% and activity 32.8 U/g. Immobilized HRP on MABs (HRP-MABs) was then used to catalyze degradation of two anthraquinonic dyes, Acid Blue 225 (AB225) and Acid Violet 109 (AV109), as models for wastewater pollutants. HRP-MABs decolorized 77.3% and 76.1% of AV109 and AB225, respectively after 15 min under optimal conditions (0.097 mM H2O2, 200 mg of HRP-MABs (8.9 mg/gcarrier), 0.08 and 0.1 g/mg beads/dye ratio for AV109 and AB225, respectively). Biocatalyst was used for 7 repeated cycles retaining 75% and 51% of initial activity for AB225 and AV109, respectively, showing potential for use in large scale applications for colored wastewater treatment.",
publisher = "MDPI",
journal = "Polymers",
title = "Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation",
volume = "14",
number = "13",
pages = "2614",
doi = "10.3390/polym14132614"
}

Jonović, M., Jugović, B., Žuža, M., Đorđević, V., Milašinović, N., Bugarski, B.,& Knežević-Jugović, Z.. (2022). Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation. in Polymers
MDPI., 14(13), 2614.
https://doi.org/10.3390/polym14132614

Jonović M, Jugović B, Žuža M, Đorđević V, Milašinović N, Bugarski B, Knežević-Jugović Z. Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation. in Polymers. 2022;14(13):2614.
doi:10.3390/polym14132614 .

Jonović, Marko, Jugović, Branimir, Žuža, Milena, Đorđević, Verica, Milašinović, Nikola, Bugarski, Branko, Knežević-Jugović, Zorica, "Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation" in Polymers, 14, no. 13 (2022):2614,
https://doi.org/10.3390/polym14132614 . .

TY  - GEN
AU  - Milašinović, Veljko
AU  - Milašinović, Dragoslav
AU  - Milašinović, Mijat
AU  - Milašinović, Nikola
AU  - Jevremović, Aleksandar
AU  - Raić, Karlo
AU  - Alil, Ana
AU  - Dikić, Stefan
AU  - Vlahović, Milica
AU  - Martinović, Sanja
AU  - Gligorijević, Bojan
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5963
AB  - Al/Cu konektori [ a) Al/Cu bimetalne papučice, b) Al/Cu bimetalne čaure, c) Al/Cu bimetalne završnice] nastaju kontinualnim zavarivanjem trenjem Al šipke i Cu šipke. Postupak se odvija tako što se aluminijumska šipka prethodno odsečena na potrebnu dužinu stavlja u steznu glavu koja je preko elektromagnetne spojnice i kaišnog prenosnika povezana sa pogonskim elektromotorom koji obezbeđuje rotaciju. Broj obrtaja je određen kombinacijom odgovarajućih kaišnika. Bakarna šipka, prethodno isečena na odgovarajuću dužinu se stavlja u steznu glavu koja je povezana sa hidrauličnim cilindrom koji obezbeđuje potrebni pritisak. Proces se odvija u dve faze, faza trenja i faza utiskivanja.
PB  - Tehnološko-metalurški fakultet u Beogradu, Univerzitet u Beogradu
T1  - Побољшање електро-механичких својстава Al/Cu електричних конектора смањењем интерметалног слоја, поступком редуковања времена заваривања трењем
UR  - https://hdl.handle.net/21.15107/rcub_cer_5963
ER  - 

@misc{
author = "Milašinović, Veljko and Milašinović, Dragoslav and Milašinović, Mijat and Milašinović, Nikola and Jevremović, Aleksandar and Raić, Karlo and Alil, Ana and Dikić, Stefan and Vlahović, Milica and Martinović, Sanja and Gligorijević, Bojan",
year = "2019",
abstract = "Al/Cu konektori [ a) Al/Cu bimetalne papučice, b) Al/Cu bimetalne čaure, c) Al/Cu bimetalne završnice] nastaju kontinualnim zavarivanjem trenjem Al šipke i Cu šipke. Postupak se odvija tako što se aluminijumska šipka prethodno odsečena na potrebnu dužinu stavlja u steznu glavu koja je preko elektromagnetne spojnice i kaišnog prenosnika povezana sa pogonskim elektromotorom koji obezbeđuje rotaciju. Broj obrtaja je određen kombinacijom odgovarajućih kaišnika. Bakarna šipka, prethodno isečena na odgovarajuću dužinu se stavlja u steznu glavu koja je povezana sa hidrauličnim cilindrom koji obezbeđuje potrebni pritisak. Proces se odvija u dve faze, faza trenja i faza utiskivanja.",
publisher = "Tehnološko-metalurški fakultet u Beogradu, Univerzitet u Beogradu",
title = "Побољшање електро-механичких својстава Al/Cu електричних конектора смањењем интерметалног слоја, поступком редуковања времена заваривања трењем",
url = "https://hdl.handle.net/21.15107/rcub_cer_5963"
}

Milašinović, V., Milašinović, D., Milašinović, M., Milašinović, N., Jevremović, A., Raić, K., Alil, A., Dikić, S., Vlahović, M., Martinović, S.,& Gligorijević, B.. (2019). Побољшање електро-механичких својстава Al/Cu електричних конектора смањењем интерметалног слоја, поступком редуковања времена заваривања трењем. 
Tehnološko-metalurški fakultet u Beogradu, Univerzitet u Beogradu..
https://hdl.handle.net/21.15107/rcub_cer_5963

Milašinović V, Milašinović D, Milašinović M, Milašinović N, Jevremović A, Raić K, Alil A, Dikić S, Vlahović M, Martinović S, Gligorijević B. Побољшање електро-механичких својстава Al/Cu електричних конектора смањењем интерметалног слоја, поступком редуковања времена заваривања трењем. 2019;.
https://hdl.handle.net/21.15107/rcub_cer_5963 .

Milašinović, Veljko, Milašinović, Dragoslav, Milašinović, Mijat, Milašinović, Nikola, Jevremović, Aleksandar, Raić, Karlo, Alil, Ana, Dikić, Stefan, Vlahović, Milica, Martinović, Sanja, Gligorijević, Bojan, "Побољшање електро-механичких својстава Al/Cu електричних конектора смањењем интерметалног слоја, поступком редуковања времена заваривања трењем" (2019),
https://hdl.handle.net/21.15107/rcub_cer_5963 .