TY  - JOUR
AU  - Pećić, Marija
AU  - Popović, Slađana
AU  - Milutinović, Vesna
AU  - Subakov Simić, Gordana
AU  - Trbojević, Ivana
AU  - Predojević, Dragana
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4027
AB  - Phosphorus overenrichment of shallow ponds prevailing in wetlands leads to their eutrophication causing the collapse of those vulnerable habitats. The potential of phosphorus accumulation by periphyton developed on artificial substrata has been investigated in two shallow ponds (Baračka and Široki Rit) in northwest Serbia and compared to the same ability of plankton and metaphyton. The periphyton substrate carrier has been submerged from May to October. Both continuously (CS) and monthly developed (MS) periphyton were sampled. Autotrophic component of all investigated communities has been qualitatively assessed. Maximum accumulation of only 14.7 mg TP/m2 was recorded in three-month exposed periphyton CS. MS exposed from July to August reached maximal 12.7 mg TP/m2. Plankton community that was characterized by more diverse and abundantly developed algal component was more effective in phosphorus accumulation (0.7 mg/g dry weight) in comparison with dominantly inorganic and diatom-dominated periphyton in Baračka. Unstable conditions caused by recent revitalization (dredging organic matter and mud from pond basin—redigging) as well as rapid desiccation of Široki Rit disabled making an unambiguous conclusion about the efficiency of phosphorus accumulation among different communities, but suggested slight potential of phosphorus harvesting by metaphyton in this pond. Due to the shorter exposure time that brings the reduced risk of unpredictable changes in the ecosystem, as well as the considerable amount of accumulated phosphorus, large-scale application of one-month exposed periphyton developed on artificial substrates would be more advisable for phosphorus harvesting in nutrient affected shallow ponds.
PB  - Springer Science and Business Media LLC
T2  - Journal of Oceanology and Limnology
T1  - Efficiency of phosphorus accumulation by plankton, periphyton developed on submerged artificial substrata and metaphyton: in-situ observation in two shallow ponds
DO  - 10.1007/s00343-020-0116-4
ER  - 

@article{
author = "Pećić, Marija and Popović, Slađana and Milutinović, Vesna and Subakov Simić, Gordana and Trbojević, Ivana and Predojević, Dragana",
year = "2020",
abstract = "Phosphorus overenrichment of shallow ponds prevailing in wetlands leads to their eutrophication causing the collapse of those vulnerable habitats. The potential of phosphorus accumulation by periphyton developed on artificial substrata has been investigated in two shallow ponds (Baračka and Široki Rit) in northwest Serbia and compared to the same ability of plankton and metaphyton. The periphyton substrate carrier has been submerged from May to October. Both continuously (CS) and monthly developed (MS) periphyton were sampled. Autotrophic component of all investigated communities has been qualitatively assessed. Maximum accumulation of only 14.7 mg TP/m2 was recorded in three-month exposed periphyton CS. MS exposed from July to August reached maximal 12.7 mg TP/m2. Plankton community that was characterized by more diverse and abundantly developed algal component was more effective in phosphorus accumulation (0.7 mg/g dry weight) in comparison with dominantly inorganic and diatom-dominated periphyton in Baračka. Unstable conditions caused by recent revitalization (dredging organic matter and mud from pond basin—redigging) as well as rapid desiccation of Široki Rit disabled making an unambiguous conclusion about the efficiency of phosphorus accumulation among different communities, but suggested slight potential of phosphorus harvesting by metaphyton in this pond. Due to the shorter exposure time that brings the reduced risk of unpredictable changes in the ecosystem, as well as the considerable amount of accumulated phosphorus, large-scale application of one-month exposed periphyton developed on artificial substrates would be more advisable for phosphorus harvesting in nutrient affected shallow ponds.",
publisher = "Springer Science and Business Media LLC",
journal = "Journal of Oceanology and Limnology",
title = "Efficiency of phosphorus accumulation by plankton, periphyton developed on submerged artificial substrata and metaphyton: in-situ observation in two shallow ponds",
doi = "10.1007/s00343-020-0116-4"
}

Pećić, M., Popović, S., Milutinović, V., Subakov Simić, G., Trbojević, I.,& Predojević, D.. (2020). Efficiency of phosphorus accumulation by plankton, periphyton developed on submerged artificial substrata and metaphyton: in-situ observation in two shallow ponds. in Journal of Oceanology and Limnology
Springer Science and Business Media LLC..
https://doi.org/10.1007/s00343-020-0116-4

Pećić M, Popović S, Milutinović V, Subakov Simić G, Trbojević I, Predojević D. Efficiency of phosphorus accumulation by plankton, periphyton developed on submerged artificial substrata and metaphyton: in-situ observation in two shallow ponds. in Journal of Oceanology and Limnology. 2020;.
doi:10.1007/s00343-020-0116-4 .

Pećić, Marija, Popović, Slađana, Milutinović, Vesna, Subakov Simić, Gordana, Trbojević, Ivana, Predojević, Dragana, "Efficiency of phosphorus accumulation by plankton, periphyton developed on submerged artificial substrata and metaphyton: in-situ observation in two shallow ponds" in Journal of Oceanology and Limnology (2020),
https://doi.org/10.1007/s00343-020-0116-4 . .

TY  - JOUR
AU  - Popović, Slađana
AU  - Krizmanić, Jelena
AU  - Vidaković, Danijela
AU  - Karadžić, Vesna
AU  - Milovanović, Željka
AU  - Pećić, Marija
AU  - Subakov Simić, Gordana
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4025
AB  - Domination of certain aerophytic phototrophic group or specific taxon in biofilms is connected with biofilm features recognised in situ. Well-developed, gelatinous, olive to dark-green biofilms are composed mostly of coccoid cyanobacterial forms. The same features, characterised biofilms dominated by one coccoid taxon, except the latter were vividly coloured. Gloeobacter caused the appearance of purple, Gloeocapsa representatives yellow and Chroococcidiopsis black biofilm. The brown to the dark colour of heterocytous biofilms was mainly caused by Nostoc. Simple trichal Cyanobacteria were occasionally present in biofilm, except in one blue-coloured sample. According to the principal component analysis (PCA), well-developed and gelatinous biofilms were correlated with Cyanobacteria, while scanning electron microscopy (SEM) revealed richness of extracellular polymeric substances (EPS) in such biofilms. Biofilm with calcified cyanobacterium (Geitleria cf. calcarea) was also found. Chlorophyta-abundant biofilms (many rich in Desmococcus), thinner than cyanobacterial, were predominantly green and occasionally yellow and blue. Many were dry when observed in situ (confirmed with PCA), with few being moistened (i.e. Klebsormidium-dominant). Diatom biofilms were usually developed on sediment, mosses or near seeping water (demonstrated by PCA) and were also thinner than cyanobacterial ones. Compared to cyanobacterial biofilms, SEM showed less developed EPS in those rich in diatoms and green algae, where microorganisms are more exposed to the environment. The study demonstrates an easy method for biofilm assessment based on visual characterisation and provides encouragement for more frequent biofilm investigation in caves that can be important from an ecological, biological, biotechnological point of view and which assessment can have an irreplaceable role in potential monitoring and protection.
PB  - Springer Science and Business Media LLC
T2  - Environmental Monitoring and Assessment
T1  - Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ
VL  - 192
IS  - 11
SP  - 720
DO  - 10.1007/s10661-020-08686-4
ER  - 

@article{
author = "Popović, Slađana and Krizmanić, Jelena and Vidaković, Danijela and Karadžić, Vesna and Milovanović, Željka and Pećić, Marija and Subakov Simić, Gordana",
year = "2020",
abstract = "Domination of certain aerophytic phototrophic group or specific taxon in biofilms is connected with biofilm features recognised in situ. Well-developed, gelatinous, olive to dark-green biofilms are composed mostly of coccoid cyanobacterial forms. The same features, characterised biofilms dominated by one coccoid taxon, except the latter were vividly coloured. Gloeobacter caused the appearance of purple, Gloeocapsa representatives yellow and Chroococcidiopsis black biofilm. The brown to the dark colour of heterocytous biofilms was mainly caused by Nostoc. Simple trichal Cyanobacteria were occasionally present in biofilm, except in one blue-coloured sample. According to the principal component analysis (PCA), well-developed and gelatinous biofilms were correlated with Cyanobacteria, while scanning electron microscopy (SEM) revealed richness of extracellular polymeric substances (EPS) in such biofilms. Biofilm with calcified cyanobacterium (Geitleria cf. calcarea) was also found. Chlorophyta-abundant biofilms (many rich in Desmococcus), thinner than cyanobacterial, were predominantly green and occasionally yellow and blue. Many were dry when observed in situ (confirmed with PCA), with few being moistened (i.e. Klebsormidium-dominant). Diatom biofilms were usually developed on sediment, mosses or near seeping water (demonstrated by PCA) and were also thinner than cyanobacterial ones. Compared to cyanobacterial biofilms, SEM showed less developed EPS in those rich in diatoms and green algae, where microorganisms are more exposed to the environment. The study demonstrates an easy method for biofilm assessment based on visual characterisation and provides encouragement for more frequent biofilm investigation in caves that can be important from an ecological, biological, biotechnological point of view and which assessment can have an irreplaceable role in potential monitoring and protection.",
publisher = "Springer Science and Business Media LLC",
journal = "Environmental Monitoring and Assessment",
title = "Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ",
volume = "192",
number = "11",
pages = "720",
doi = "10.1007/s10661-020-08686-4"
}

Popović, S., Krizmanić, J., Vidaković, D., Karadžić, V., Milovanović, Ž., Pećić, M.,& Subakov Simić, G.. (2020). Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ. in Environmental Monitoring and Assessment
Springer Science and Business Media LLC., 192(11), 720.
https://doi.org/10.1007/s10661-020-08686-4

Popović S, Krizmanić J, Vidaković D, Karadžić V, Milovanović Ž, Pećić M, Subakov Simić G. Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ. in Environmental Monitoring and Assessment. 2020;192(11):720.
doi:10.1007/s10661-020-08686-4 .

Popović, Slađana, Krizmanić, Jelena, Vidaković, Danijela, Karadžić, Vesna, Milovanović, Željka, Pećić, Marija, Subakov Simić, Gordana, "Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ" in Environmental Monitoring and Assessment, 192, no. 11 (2020):720,
https://doi.org/10.1007/s10661-020-08686-4 . .

TY  - CONF
AU  - Popović, Slađana
AU  - Krizmanić, Jelena
AU  - Vidaković, Danijela
AU  - Karadžić, Vesna
AU  - Nikolić, Nataša
AU  - Pećić, Marija
AU  - Subakov-Simić, Gordana
PY  - 2019
UR  - http://www.dinaric-symposium.org/file/AbstractBook_FINAL.pdf
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4617
AB  - Phototrophic biofilms can be found in different habitats, including subterranean ones, such as caves. In caves, they thrive in the presence of daylight at the entrances, or in the vicinity of artificial light inside, and are usually very heterogeneous. For the purpose of this study, biofilm sampling from stone substrata was performed in 15 caves (mostly entrances) to examine biofilm characteristic and features, as well as to record the main phototrophic groups. Cyanobacteria, Bacillariophyta and Chlorophyta were the main phototrophs recorded. Cyanobacteria were, according to morphological characteristics, additionally divided into coccoid, simple trichal and heterocytous forms. Biofilms were sorted according to the degree of development and moisture (taking into account free seeping water and water bound in extracellular polymeric substances (EPSs)). Other parameters, namely the water content and content of organic/inorganic matter were determined too. According to the principal component analysis (PCA), Bacillariophyta and Chlorophyta were usually dominant in thin biofilms: while Chlorophyta were connected with dry thin biofilms, Bacillariophyta were abundant in thin biofilms constantly moistened by seeping water. Cyanobacteria dominated in thick and EPSs rich biofilms that also were positively correlated with light intensity and higher values of chlorophyll a. Biofilms were also sorted according to colour, where certain colours or colour assemblages corresponded to whole divisions, cyanobacterial groups or even only one taxon. Chlorophyta rich biofilms were mostly green, only few of them yellow and orange, while those where Cyanobacteria accounted as the most abundant, were very colourful (red, purple, blue, yellow, brown), for which usually only one species was responsible. Scanning electron microscopy (SEM) has additionally helped us to distinguish different biofilm types and their morphology better and enabled detailed analysis of distribution, shape, size and orientation of biofilm constituents. The biofilm rich in Cyanobacteria with calcified filaments occurred as particularly interesting for SEM exploration.
PB  - Postojna, Slovenia : Organizing Commitee, 2nd Dinaric Symposium on Subterranean Biology
C3  - Abstract book - 2nd Dinaric Symposium on Subterranean Biology
T1  - Phototrophic biofilms in Serbian caves: exploration and features
SP  - 48
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_cer_4617
ER  - 

@conference{
author = "Popović, Slađana and Krizmanić, Jelena and Vidaković, Danijela and Karadžić, Vesna and Nikolić, Nataša and Pećić, Marija and Subakov-Simić, Gordana",
year = "2019",
abstract = "Phototrophic biofilms can be found in different habitats, including subterranean ones, such as caves. In caves, they thrive in the presence of daylight at the entrances, or in the vicinity of artificial light inside, and are usually very heterogeneous. For the purpose of this study, biofilm sampling from stone substrata was performed in 15 caves (mostly entrances) to examine biofilm characteristic and features, as well as to record the main phototrophic groups. Cyanobacteria, Bacillariophyta and Chlorophyta were the main phototrophs recorded. Cyanobacteria were, according to morphological characteristics, additionally divided into coccoid, simple trichal and heterocytous forms. Biofilms were sorted according to the degree of development and moisture (taking into account free seeping water and water bound in extracellular polymeric substances (EPSs)). Other parameters, namely the water content and content of organic/inorganic matter were determined too. According to the principal component analysis (PCA), Bacillariophyta and Chlorophyta were usually dominant in thin biofilms: while Chlorophyta were connected with dry thin biofilms, Bacillariophyta were abundant in thin biofilms constantly moistened by seeping water. Cyanobacteria dominated in thick and EPSs rich biofilms that also were positively correlated with light intensity and higher values of chlorophyll a. Biofilms were also sorted according to colour, where certain colours or colour assemblages corresponded to whole divisions, cyanobacterial groups or even only one taxon. Chlorophyta rich biofilms were mostly green, only few of them yellow and orange, while those where Cyanobacteria accounted as the most abundant, were very colourful (red, purple, blue, yellow, brown), for which usually only one species was responsible. Scanning electron microscopy (SEM) has additionally helped us to distinguish different biofilm types and their morphology better and enabled detailed analysis of distribution, shape, size and orientation of biofilm constituents. The biofilm rich in Cyanobacteria with calcified filaments occurred as particularly interesting for SEM exploration.",
publisher = "Postojna, Slovenia : Organizing Commitee, 2nd Dinaric Symposium on Subterranean Biology",
journal = "Abstract book - 2nd Dinaric Symposium on Subterranean Biology",
title = "Phototrophic biofilms in Serbian caves: exploration and features",
pages = "48-48",
url = "https://hdl.handle.net/21.15107/rcub_cer_4617"
}

Popović, S., Krizmanić, J., Vidaković, D., Karadžić, V., Nikolić, N., Pećić, M.,& Subakov-Simić, G.. (2019). Phototrophic biofilms in Serbian caves: exploration and features. in Abstract book - 2nd Dinaric Symposium on Subterranean Biology
Postojna, Slovenia : Organizing Commitee, 2nd Dinaric Symposium on Subterranean Biology., 48-48.
https://hdl.handle.net/21.15107/rcub_cer_4617

Popović S, Krizmanić J, Vidaković D, Karadžić V, Nikolić N, Pećić M, Subakov-Simić G. Phototrophic biofilms in Serbian caves: exploration and features. in Abstract book - 2nd Dinaric Symposium on Subterranean Biology. 2019;:48-48.
https://hdl.handle.net/21.15107/rcub_cer_4617 .

Popović, Slađana, Krizmanić, Jelena, Vidaković, Danijela, Karadžić, Vesna, Nikolić, Nataša, Pećić, Marija, Subakov-Simić, Gordana, "Phototrophic biofilms in Serbian caves: exploration and features" in Abstract book - 2nd Dinaric Symposium on Subterranean Biology (2019):48-48,
https://hdl.handle.net/21.15107/rcub_cer_4617 .