TY  - JOUR
AU  - Radakovic, M.
AU  - Davitkov, D.
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
AU  - Stevanović, Jevrosima
AU  - Krstic, V.
AU  - Stanimirovic, Z.
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1947
AB  - The aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2-), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH). In addition, relative distribution of lactate dehydrogenase (LDH1-LDH5) activity and the DNA-damaging effects of T. equi infection were evaluated. Compared to control horses, horses infected with T. equi had significantly higher SOD1 activities (P  LT  0.05) and PHb (P  LT  0.01), NO2- (P  LT  0.001), NOx (P  LT  0.05) and MDA concentrations (P  LT  0.001), and significantly-lower EH (P  LT  0.001), CAT (P  LT  0.01) and PON1 (P  LT  0.001) activities, and thiol group concentrations (P  LT  0.05). The comet assay demonstrated significantly increased DNA damage in T. equi infected cells compared to non-infected cells (P  LT  0.001). Infected horses had significantly increased LDH5 isoenzyme activities (P  LT  0.05). There was higher production of ROS/RNS in T. equi-infected horses, which resulted in changes in osmotic fragility, damage to lipids, proteins and DNA, haemolysis and hepatocellular damage. Oxidative stress in horses naturally infected with T equi could contribute to the pathogenesis of the infection.
PB  - Elsevier Sci Ltd, Oxford
T2  - Veterinary Journal
T1  - Oxidative stress and DNA damage in horses naturally infected with Theileria equi
VL  - 217
SP  - 112
EP  - 118
DO  - 10.1016/j.tvjl.2016.10.003
ER  - 

@article{
author = "Radakovic, M. and Davitkov, D. and Borozan, Sunčica and Stojanović, Srđan and Stevanović, Jevrosima and Krstic, V. and Stanimirovic, Z.",
year = "2016",
abstract = "The aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2-), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH). In addition, relative distribution of lactate dehydrogenase (LDH1-LDH5) activity and the DNA-damaging effects of T. equi infection were evaluated. Compared to control horses, horses infected with T. equi had significantly higher SOD1 activities (P  LT  0.05) and PHb (P  LT  0.01), NO2- (P  LT  0.001), NOx (P  LT  0.05) and MDA concentrations (P  LT  0.001), and significantly-lower EH (P  LT  0.001), CAT (P  LT  0.01) and PON1 (P  LT  0.001) activities, and thiol group concentrations (P  LT  0.05). The comet assay demonstrated significantly increased DNA damage in T. equi infected cells compared to non-infected cells (P  LT  0.001). Infected horses had significantly increased LDH5 isoenzyme activities (P  LT  0.05). There was higher production of ROS/RNS in T. equi-infected horses, which resulted in changes in osmotic fragility, damage to lipids, proteins and DNA, haemolysis and hepatocellular damage. Oxidative stress in horses naturally infected with T equi could contribute to the pathogenesis of the infection.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Veterinary Journal",
title = "Oxidative stress and DNA damage in horses naturally infected with Theileria equi",
volume = "217",
pages = "112-118",
doi = "10.1016/j.tvjl.2016.10.003"
}

Radakovic, M., Davitkov, D., Borozan, S., Stojanović, S., Stevanović, J., Krstic, V.,& Stanimirovic, Z.. (2016). Oxidative stress and DNA damage in horses naturally infected with Theileria equi. in Veterinary Journal
Elsevier Sci Ltd, Oxford., 217, 112-118.
https://doi.org/10.1016/j.tvjl.2016.10.003

Radakovic M, Davitkov D, Borozan S, Stojanović S, Stevanović J, Krstic V, Stanimirovic Z. Oxidative stress and DNA damage in horses naturally infected with Theileria equi. in Veterinary Journal. 2016;217:112-118.
doi:10.1016/j.tvjl.2016.10.003 .

Radakovic, M., Davitkov, D., Borozan, Sunčica, Stojanović, Srđan, Stevanović, Jevrosima, Krstic, V., Stanimirovic, Z., "Oxidative stress and DNA damage in horses naturally infected with Theileria equi" in Veterinary Journal, 217 (2016):112-118,
https://doi.org/10.1016/j.tvjl.2016.10.003 . .

TY  - JOUR
AU  - Radakovic, M.
AU  - Davitkov, D.
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
AU  - Stevanović, Jevrosima
AU  - Krstic, V.
AU  - Stanimirovic, Z.
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4383
AB  - The aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2-), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH). In addition, relative distribution of lactate dehydrogenase (LDH1-LDH5) activity and the DNA-damaging effects of T. equi infection were evaluated. Compared to control horses, horses infected with T. equi had significantly higher SOD1 activities (P  LT  0.05) and PHb (P  LT  0.01), NO2- (P  LT  0.001), NOx (P  LT  0.05) and MDA concentrations (P  LT  0.001), and significantly-lower EH (P  LT  0.001), CAT (P  LT  0.01) and PON1 (P  LT  0.001) activities, and thiol group concentrations (P  LT  0.05). The comet assay demonstrated significantly increased DNA damage in T. equi infected cells compared to non-infected cells (P  LT  0.001). Infected horses had significantly increased LDH5 isoenzyme activities (P  LT  0.05). There was higher production of ROS/RNS in T. equi-infected horses, which resulted in changes in osmotic fragility, damage to lipids, proteins and DNA, haemolysis and hepatocellular damage. Oxidative stress in horses naturally infected with T equi could contribute to the pathogenesis of the infection.
PB  - Elsevier
T2  - Veterinary Journal
T1  - Oxidative stress and DNA damage in horses naturally infected with Theileria equi
VL  - 217
SP  - 112
EP  - 118
DO  - 10.1016/j.tvjl.2016.10.003
ER  - 

@article{
author = "Radakovic, M. and Davitkov, D. and Borozan, Sunčica and Stojanović, Srđan and Stevanović, Jevrosima and Krstic, V. and Stanimirovic, Z.",
year = "2016",
abstract = "The aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2-), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH). In addition, relative distribution of lactate dehydrogenase (LDH1-LDH5) activity and the DNA-damaging effects of T. equi infection were evaluated. Compared to control horses, horses infected with T. equi had significantly higher SOD1 activities (P  LT  0.05) and PHb (P  LT  0.01), NO2- (P  LT  0.001), NOx (P  LT  0.05) and MDA concentrations (P  LT  0.001), and significantly-lower EH (P  LT  0.001), CAT (P  LT  0.01) and PON1 (P  LT  0.001) activities, and thiol group concentrations (P  LT  0.05). The comet assay demonstrated significantly increased DNA damage in T. equi infected cells compared to non-infected cells (P  LT  0.001). Infected horses had significantly increased LDH5 isoenzyme activities (P  LT  0.05). There was higher production of ROS/RNS in T. equi-infected horses, which resulted in changes in osmotic fragility, damage to lipids, proteins and DNA, haemolysis and hepatocellular damage. Oxidative stress in horses naturally infected with T equi could contribute to the pathogenesis of the infection.",
publisher = "Elsevier",
journal = "Veterinary Journal",
title = "Oxidative stress and DNA damage in horses naturally infected with Theileria equi",
volume = "217",
pages = "112-118",
doi = "10.1016/j.tvjl.2016.10.003"
}

Radakovic, M., Davitkov, D., Borozan, S., Stojanović, S., Stevanović, J., Krstic, V.,& Stanimirovic, Z.. (2016). Oxidative stress and DNA damage in horses naturally infected with Theileria equi. in Veterinary Journal
Elsevier., 217, 112-118.
https://doi.org/10.1016/j.tvjl.2016.10.003

Radakovic M, Davitkov D, Borozan S, Stojanović S, Stevanović J, Krstic V, Stanimirovic Z. Oxidative stress and DNA damage in horses naturally infected with Theileria equi. in Veterinary Journal. 2016;217:112-118.
doi:10.1016/j.tvjl.2016.10.003 .

Radakovic, M., Davitkov, D., Borozan, Sunčica, Stojanović, Srđan, Stevanović, Jevrosima, Krstic, V., Stanimirovic, Z., "Oxidative stress and DNA damage in horses naturally infected with Theileria equi" in Veterinary Journal, 217 (2016):112-118,
https://doi.org/10.1016/j.tvjl.2016.10.003 . .