TY  - JOUR
AU  - Unković, N
AU  - Dimkić, I
AU  - Stanković, Slaviša
AU  - Jelikić, A
AU  - Stanojević, D
AU  - Popović, Slađana
AU  - Stupar, M
AU  - Vukojević, J
AU  - Grbić, M L
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2928
AB  - The main purpose of this study was to isolate airborne fungi and assess seasonal variations in air contamination with their particulates by determining the levels of their propagules in the nave and exonarthex of a church. We also monitored indoor microclimate as a determining factor for fungal proliferation on wall paintings, spore release, and transmission through the air. The temperature and relative humidity of the nave favoured fungal growth. A total of 33 fungi were isolated, mainly of the phylum Ascomycota, and to the lesser extent of the phyla Zygomycota and Basidiomycota. The most common were the fungi of the genera Penicillium and Aspergillus (23.55 % and 20.58 %, respectively). Sørensen's quotient of similarity (0.37) suggests moderate species overlap and constant exchange of fungal propagules between the nave and exonarthex. The autumn had the highest diversity, with 17 documented taxa, followed by the summer and the winter. The spring had only eight taxa. Quantitative analysis of the airborne mycobiota in the nave (430±84.85 to 1880±106.07 CFU m -3 ) and exonarthex (715±59.62 to 2295±91.92 CFU m -3 ) showed very high contamination throughout the year, with values exceeding the maximum permissible concentrations by most standards. Many of the fungi determined in this study are known for their biodeteriogenic, toxigenic, and allergenic properties, and are a threat not only to occasional visitors and staff, but also to valuable works of art decorating nave walls. © 2018 Sciendo. All rights reserved. All Rights Reserved.
AB  - Glavni cilj ovoga istraživanja bio je izolirati gljive iz zraka i procijeniti sezonske promjene u onečišćenju zraka gljivičnim propagulama u naosu i egzonarteksu istraživane crkve. Također su praćeni mikroklimatski parametri kao ograničavajući čimbenici za razvoj i rast gljiva na zidnim slikama, za oslobađanje spora i njihovu transmisiju kroz zrak. Zabilježena temperatura i relativna vlažnost zraka u naosu pogodovale su razvoju i rastu gljiva. Dokumentirana su 33 taksona gljiva, uglavnom pripadnika koljena Ascomycota, a manje su zastupljeni oni Zygomycota i Basidiomycota. Najčešće su bile prisutne plijesni rodova Penicillium (23,55 %) i Aspergillus (20,58 %). Sørensenov indeks sličnosti (0,37) upućuje na stalnu i umjerenu razmjenu gljivičnih propagula između naosa i egzonarteksa. Uzorci uzeti u jesen pokazali su najveću raznolikost sa 17 zabilježenih taksona, a oni uzeti u proljeće samo osam taksona. Kvantitativna mikološka analiza u naosu (430±84,85 do 1880±106,07 CFU m-3) i egzonarteksu (715±59,62 do 2295±91,92 CFU m-3) pokazala je visoku godišnju razinu onečišćenja zraka, s vrijednostima koje prema većini standarda prelaze dopuštene koncentracije. Mnoge identificirane gljive mogu dovesti do biodeterioracije, proizvesti mikotoksine i izazvati alergijske reakcije. Stoga su nepoželjne u sakralnim objektima ne samo zbog očuvanja murala nego i zbog zaštite zdravlja zaposlenih, posjetitelja i konzervatora.
PB  - Sciendo
T2  - Arhiv za Higijenu Rada i Toksikologiju
T2  - Arhiv za Higijenu Rada i Toksikologiju
T1  - Seasonal diversity of biodeteriogenic, pathogenic, and toxigenic constituents of airborne mycobiota in a sacral environment
T1  - Sezonska raznovrsnost biodeteriogenih, patogenih i toksigenih gljiva u zraku sakralnoga objekta
VL  - 69
IS  - 4
SP  - 317
EP  - 327
DO  - 10.2478/aiht-2018-69-3194
ER  - 

@article{
author = "Unković, N and Dimkić, I and Stanković, Slaviša and Jelikić, A and Stanojević, D and Popović, Slađana and Stupar, M and Vukojević, J and Grbić, M L",
year = "2018",
abstract = "The main purpose of this study was to isolate airborne fungi and assess seasonal variations in air contamination with their particulates by determining the levels of their propagules in the nave and exonarthex of a church. We also monitored indoor microclimate as a determining factor for fungal proliferation on wall paintings, spore release, and transmission through the air. The temperature and relative humidity of the nave favoured fungal growth. A total of 33 fungi were isolated, mainly of the phylum Ascomycota, and to the lesser extent of the phyla Zygomycota and Basidiomycota. The most common were the fungi of the genera Penicillium and Aspergillus (23.55 % and 20.58 %, respectively). Sørensen's quotient of similarity (0.37) suggests moderate species overlap and constant exchange of fungal propagules between the nave and exonarthex. The autumn had the highest diversity, with 17 documented taxa, followed by the summer and the winter. The spring had only eight taxa. Quantitative analysis of the airborne mycobiota in the nave (430±84.85 to 1880±106.07 CFU m -3 ) and exonarthex (715±59.62 to 2295±91.92 CFU m -3 ) showed very high contamination throughout the year, with values exceeding the maximum permissible concentrations by most standards. Many of the fungi determined in this study are known for their biodeteriogenic, toxigenic, and allergenic properties, and are a threat not only to occasional visitors and staff, but also to valuable works of art decorating nave walls. © 2018 Sciendo. All rights reserved. All Rights Reserved., Glavni cilj ovoga istraživanja bio je izolirati gljive iz zraka i procijeniti sezonske promjene u onečišćenju zraka gljivičnim propagulama u naosu i egzonarteksu istraživane crkve. Također su praćeni mikroklimatski parametri kao ograničavajući čimbenici za razvoj i rast gljiva na zidnim slikama, za oslobađanje spora i njihovu transmisiju kroz zrak. Zabilježena temperatura i relativna vlažnost zraka u naosu pogodovale su razvoju i rastu gljiva. Dokumentirana su 33 taksona gljiva, uglavnom pripadnika koljena Ascomycota, a manje su zastupljeni oni Zygomycota i Basidiomycota. Najčešće su bile prisutne plijesni rodova Penicillium (23,55 %) i Aspergillus (20,58 %). Sørensenov indeks sličnosti (0,37) upućuje na stalnu i umjerenu razmjenu gljivičnih propagula između naosa i egzonarteksa. Uzorci uzeti u jesen pokazali su najveću raznolikost sa 17 zabilježenih taksona, a oni uzeti u proljeće samo osam taksona. Kvantitativna mikološka analiza u naosu (430±84,85 do 1880±106,07 CFU m-3) i egzonarteksu (715±59,62 do 2295±91,92 CFU m-3) pokazala je visoku godišnju razinu onečišćenja zraka, s vrijednostima koje prema većini standarda prelaze dopuštene koncentracije. Mnoge identificirane gljive mogu dovesti do biodeterioracije, proizvesti mikotoksine i izazvati alergijske reakcije. Stoga su nepoželjne u sakralnim objektima ne samo zbog očuvanja murala nego i zbog zaštite zdravlja zaposlenih, posjetitelja i konzervatora.",
publisher = "Sciendo",
journal = "Arhiv za Higijenu Rada i Toksikologiju, Arhiv za Higijenu Rada i Toksikologiju",
title = "Seasonal diversity of biodeteriogenic, pathogenic, and toxigenic constituents of airborne mycobiota in a sacral environment, Sezonska raznovrsnost biodeteriogenih, patogenih i toksigenih gljiva u zraku sakralnoga objekta",
volume = "69",
number = "4",
pages = "317-327",
doi = "10.2478/aiht-2018-69-3194"
}

Unković, N., Dimkić, I., Stanković, S., Jelikić, A., Stanojević, D., Popović, S., Stupar, M., Vukojević, J.,& Grbić, M. L.. (2018). Seasonal diversity of biodeteriogenic, pathogenic, and toxigenic constituents of airborne mycobiota in a sacral environment. in Arhiv za Higijenu Rada i Toksikologiju
Sciendo., 69(4), 317-327.
https://doi.org/10.2478/aiht-2018-69-3194

Unković N, Dimkić I, Stanković S, Jelikić A, Stanojević D, Popović S, Stupar M, Vukojević J, Grbić ML. Seasonal diversity of biodeteriogenic, pathogenic, and toxigenic constituents of airborne mycobiota in a sacral environment. in Arhiv za Higijenu Rada i Toksikologiju. 2018;69(4):317-327.
doi:10.2478/aiht-2018-69-3194 .

Unković, N, Dimkić, I, Stanković, Slaviša, Jelikić, A, Stanojević, D, Popović, Slađana, Stupar, M, Vukojević, J, Grbić, M L, "Seasonal diversity of biodeteriogenic, pathogenic, and toxigenic constituents of airborne mycobiota in a sacral environment" in Arhiv za Higijenu Rada i Toksikologiju, 69, no. 4 (2018):317-327,
https://doi.org/10.2478/aiht-2018-69-3194 . .

TY  - JOUR
AU  - Popović, Slađana
AU  - Simić, Gordana
AU  - Stupar, M.
AU  - Unković, N.
AU  - Krunić, O.
AU  - Savić, N.
AU  - Grbić, M.
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2208
AB  - Cyanobacteria, algae (Chlorophyta and Bacillariophyta), and fungi were identified from biofilm samples from three caves in western Serbia: Ribnička, Hadž i Prodanova, and Rćanska. Temperature, light intensity, and relative humidity varied from 16.9 8C to 24.9 8C, 61% to 87%, and 215 Lux to 4400 Lux, respectively. In general, the highest number of documented taxa belonged to Cyanobacteria, with chroococcalean taxa prevailing and Gloeocapsa species as the most diverse. A large percentage of observed fungi were Ascomycetes or Zygomycetes, while the only representative of Basidiomycetes was Rhizoctonia s. lat. However, a redundancy analysis revealed that different taxonomic groups were dominant at different localities: cyanobacteria and fungi in Ribnička and Hadž i Prodanova, and Chlorophyta and Bacillariophyta in Rćanska. The statistical analysis showed that relative humidity is an important physical parameter influencing the development of various microbial communities in different caves. Cyanobacteria were mostly found in places with lower relative humidity, while Chlorophyta and Bacillariophyta were found in places with higher humidity. The documented physical parameters did not have a significant impact on the distribution of fungi. Measured chlorophyll-a content was highest on horizontal surfaces, where the highest content of organic/inorganic matter were also recorded. The highest water content was observed in biofilm samples from which many cyanobacteria taxa were identified.
PB  - National Speleological Society Inc.
T2  - Journal of Cave and Karst Studies
T1  - Cave biofilms: Characterization of phototrophic cyanobacteria and algae and chemotrophic fungi from three caves in Serbia
VL  - 79
IS  - 1
SP  - 10
EP  - 23
DO  - 10.4311/2016MB0124
ER  - 

@article{
author = "Popović, Slađana and Simić, Gordana and Stupar, M. and Unković, N. and Krunić, O. and Savić, N. and Grbić, M.",
year = "2017",
abstract = "Cyanobacteria, algae (Chlorophyta and Bacillariophyta), and fungi were identified from biofilm samples from three caves in western Serbia: Ribnička, Hadž i Prodanova, and Rćanska. Temperature, light intensity, and relative humidity varied from 16.9 8C to 24.9 8C, 61% to 87%, and 215 Lux to 4400 Lux, respectively. In general, the highest number of documented taxa belonged to Cyanobacteria, with chroococcalean taxa prevailing and Gloeocapsa species as the most diverse. A large percentage of observed fungi were Ascomycetes or Zygomycetes, while the only representative of Basidiomycetes was Rhizoctonia s. lat. However, a redundancy analysis revealed that different taxonomic groups were dominant at different localities: cyanobacteria and fungi in Ribnička and Hadž i Prodanova, and Chlorophyta and Bacillariophyta in Rćanska. The statistical analysis showed that relative humidity is an important physical parameter influencing the development of various microbial communities in different caves. Cyanobacteria were mostly found in places with lower relative humidity, while Chlorophyta and Bacillariophyta were found in places with higher humidity. The documented physical parameters did not have a significant impact on the distribution of fungi. Measured chlorophyll-a content was highest on horizontal surfaces, where the highest content of organic/inorganic matter were also recorded. The highest water content was observed in biofilm samples from which many cyanobacteria taxa were identified.",
publisher = "National Speleological Society Inc.",
journal = "Journal of Cave and Karst Studies",
title = "Cave biofilms: Characterization of phototrophic cyanobacteria and algae and chemotrophic fungi from three caves in Serbia",
volume = "79",
number = "1",
pages = "10-23",
doi = "10.4311/2016MB0124"
}

Popović, S., Simić, G., Stupar, M., Unković, N., Krunić, O., Savić, N.,& Grbić, M.. (2017). Cave biofilms: Characterization of phototrophic cyanobacteria and algae and chemotrophic fungi from three caves in Serbia. in Journal of Cave and Karst Studies
National Speleological Society Inc.., 79(1), 10-23.
https://doi.org/10.4311/2016MB0124

Popović S, Simić G, Stupar M, Unković N, Krunić O, Savić N, Grbić M. Cave biofilms: Characterization of phototrophic cyanobacteria and algae and chemotrophic fungi from three caves in Serbia. in Journal of Cave and Karst Studies. 2017;79(1):10-23.
doi:10.4311/2016MB0124 .

Popović, Slađana, Simić, Gordana, Stupar, M., Unković, N., Krunić, O., Savić, N., Grbić, M., "Cave biofilms: Characterization of phototrophic cyanobacteria and algae and chemotrophic fungi from three caves in Serbia" in Journal of Cave and Karst Studies, 79, no. 1 (2017):10-23,
https://doi.org/10.4311/2016MB0124 . .