TY  - JOUR
AU  - Ivković, Ivana
AU  - Novaković, Miroslav
AU  - Veljić, Milan
AU  - Mojsin, Marija
AU  - Stevanović, Milena J.
AU  - Marin, Petar D.
AU  - Bukvički, Danka
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4696
AB  - Based on previous investigations where bis-bibenzyls isolated from liverworts showed various biological activities (cytotoxic, antimicrobial, and antiviral), we investigated their cytotoxic activity in several human cancer cell lines. From the methylene-chloride/methanol extract of the liverwort Pellia endiviifolia, three bis-bibenzyls of the perrottetin type were isolated, namely perrottetin E, 10′-hydroxyperrottetin E, and 10,10′-dihydroxyperrottetin E. The last two were found for the first time in this species. Their structures were resolved using 1D and 2D NMR, as well as by comparison with data in the literature. Cytotoxic activity of the isolated compounds was tested on three human leukemia cell lines, HL-60 (acute promyelocytic leukemia cells), U-937 (acute monocytic leukemia cells), and K-562 (human chronic myelogenous leukemia cells), as well as on human embryonal teratocarcinoma cell line (NT2/D1) and human glioblastoma cell lines A-172 and U-251, and compared to the previously isolated bis-bibenzyls (perrottetins) of similar structure. The isolated compounds exhibited modest activity against leukemia cells and significant activity against NT2/D1 and A-172. Overall, the most active cytotoxic compounds in this investigation were perrottetin E (1), isolated in this work from Pellia endiviifolia, and perrottetin F phenanthrene derivative (7), previously isolated from Lunularia cruciata and added for a comparison of their cytotoxic activity.
PB  - MDPI
T2  - Plants
T1  - Bis-Bibenzyls from the Liverwort Pellia endiviifolia and Their Biological Activity
VL  - 10
IS  - 6
SP  - 1063
DO  - 10.3390/plants10061063
ER  - 

@article{
author = "Ivković, Ivana and Novaković, Miroslav and Veljić, Milan and Mojsin, Marija and Stevanović, Milena J. and Marin, Petar D. and Bukvički, Danka",
year = "2021",
abstract = "Based on previous investigations where bis-bibenzyls isolated from liverworts showed various biological activities (cytotoxic, antimicrobial, and antiviral), we investigated their cytotoxic activity in several human cancer cell lines. From the methylene-chloride/methanol extract of the liverwort Pellia endiviifolia, three bis-bibenzyls of the perrottetin type were isolated, namely perrottetin E, 10′-hydroxyperrottetin E, and 10,10′-dihydroxyperrottetin E. The last two were found for the first time in this species. Their structures were resolved using 1D and 2D NMR, as well as by comparison with data in the literature. Cytotoxic activity of the isolated compounds was tested on three human leukemia cell lines, HL-60 (acute promyelocytic leukemia cells), U-937 (acute monocytic leukemia cells), and K-562 (human chronic myelogenous leukemia cells), as well as on human embryonal teratocarcinoma cell line (NT2/D1) and human glioblastoma cell lines A-172 and U-251, and compared to the previously isolated bis-bibenzyls (perrottetins) of similar structure. The isolated compounds exhibited modest activity against leukemia cells and significant activity against NT2/D1 and A-172. Overall, the most active cytotoxic compounds in this investigation were perrottetin E (1), isolated in this work from Pellia endiviifolia, and perrottetin F phenanthrene derivative (7), previously isolated from Lunularia cruciata and added for a comparison of their cytotoxic activity.",
publisher = "MDPI",
journal = "Plants",
title = "Bis-Bibenzyls from the Liverwort Pellia endiviifolia and Their Biological Activity",
volume = "10",
number = "6",
pages = "1063",
doi = "10.3390/plants10061063"
}

Ivković, I., Novaković, M., Veljić, M., Mojsin, M., Stevanović, M. J., Marin, P. D.,& Bukvički, D.. (2021). Bis-Bibenzyls from the Liverwort Pellia endiviifolia and Their Biological Activity. in Plants
MDPI., 10(6), 1063.
https://doi.org/10.3390/plants10061063

Ivković I, Novaković M, Veljić M, Mojsin M, Stevanović MJ, Marin PD, Bukvički D. Bis-Bibenzyls from the Liverwort Pellia endiviifolia and Their Biological Activity. in Plants. 2021;10(6):1063.
doi:10.3390/plants10061063 .

Ivković, Ivana, Novaković, Miroslav, Veljić, Milan, Mojsin, Marija, Stevanović, Milena J., Marin, Petar D., Bukvički, Danka, "Bis-Bibenzyls from the Liverwort Pellia endiviifolia and Their Biological Activity" in Plants, 10, no. 6 (2021):1063,
https://doi.org/10.3390/plants10061063 . .

TY  - JOUR
AU  - Ivković, Ivana
AU  - Bukvički, Danka
AU  - Novaković, Miroslav
AU  - Ivanović, Stefan
AU  - Stanojević, Olja
AU  - Nikolić, Ivan
AU  - Veljić, Milan
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4920
AB  - The antimicrobial activity of methanol extracts of three thalloid liver­worts, Marchantia polymorpha, Conocephalum conicum and Pellia endiviifolia and bis-bibenzyl marchantin A, the most dominant compound in the methanol extract of M. polymorpha, have been investigated in this research. 1H-NMR spec­troscopy revealed that the M. polymorpha and P. endiviifolia extracts of liverwort contain terpenes, oils, sugars and bis-bibenzyls, while these specific macrocyclic compounds were absent in the C. conicum extract. The anti­mic­robial potential was tested on eight bacterial strains. Antimicrobial effects of extracts and mar­chantin A were observed against Gram-positive bacteria, while they showed no effect against Gram-negative bacteria in both methods used – well diffusion and broth microdilution.
AB  - У овом раду испитиван је хемијски састав и антибактеријска активност метанолних екстраката три јетрењачe, Marchantia polymorpha, Conocephalum conicum и Pellia endiveifolia и бис-бибензила маршанцина A, доминантне компоненте у метанолном екстракту M. polymorpha. 1H-NMR спектроскопија је показала присуство терпена, уља, шећера и бис-бибензила у екстрактима M. polymorpha и P. endiviifolia, док екстракт C. conicum не садржи специфична макроциклична једињења – бис-бибензиле. Антимикробни потен- цијал је тестиран на осам бактеријских сојева. Антимикробни ефекат маршанцина А уочен је на све грам позитивне сојеве, док је ефекат изостао код грам негативних сојева у обе тестиране методе – дифузионе методе у бунарима и микродилуционе методе у хранљивом бујону.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Antibacterial properties of thalloid liverworts Marchantia polymorpha L., Conocephalum conicum (L.) Dum. and Pellia endiviifolia (Dicks.) Dumort
T1  - Антибактеријска својства талусних јетрењача marchantia polymorpha l., conocephalum conicum (l.) dum. И pellia endiviifolia (dicks.) dumort.
IS  - 12
SP  - 1249
EP  - 1258
DO  - 10.2298/JSC210728084I
ER  - 

@article{
author = "Ivković, Ivana and Bukvički, Danka and Novaković, Miroslav and Ivanović, Stefan and Stanojević, Olja and Nikolić, Ivan and Veljić, Milan",
year = "2021",
abstract = "The antimicrobial activity of methanol extracts of three thalloid liver­worts, Marchantia polymorpha, Conocephalum conicum and Pellia endiviifolia and bis-bibenzyl marchantin A, the most dominant compound in the methanol extract of M. polymorpha, have been investigated in this research. 1H-NMR spec­troscopy revealed that the M. polymorpha and P. endiviifolia extracts of liverwort contain terpenes, oils, sugars and bis-bibenzyls, while these specific macrocyclic compounds were absent in the C. conicum extract. The anti­mic­robial potential was tested on eight bacterial strains. Antimicrobial effects of extracts and mar­chantin A were observed against Gram-positive bacteria, while they showed no effect against Gram-negative bacteria in both methods used – well diffusion and broth microdilution., У овом раду испитиван је хемијски састав и антибактеријска активност метанолних екстраката три јетрењачe, Marchantia polymorpha, Conocephalum conicum и Pellia endiveifolia и бис-бибензила маршанцина A, доминантне компоненте у метанолном екстракту M. polymorpha. 1H-NMR спектроскопија је показала присуство терпена, уља, шећера и бис-бибензила у екстрактима M. polymorpha и P. endiviifolia, док екстракт C. conicum не садржи специфична макроциклична једињења – бис-бибензиле. Антимикробни потен- цијал је тестиран на осам бактеријских сојева. Антимикробни ефекат маршанцина А уочен је на све грам позитивне сојеве, док је ефекат изостао код грам негативних сојева у обе тестиране методе – дифузионе методе у бунарима и микродилуционе методе у хранљивом бујону.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Antibacterial properties of thalloid liverworts Marchantia polymorpha L., Conocephalum conicum (L.) Dum. and Pellia endiviifolia (Dicks.) Dumort, Антибактеријска својства талусних јетрењача marchantia polymorpha l., conocephalum conicum (l.) dum. И pellia endiviifolia (dicks.) dumort.",
number = "12",
pages = "1249-1258",
doi = "10.2298/JSC210728084I"
}

Ivković, I., Bukvički, D., Novaković, M., Ivanović, S., Stanojević, O., Nikolić, I.,& Veljić, M.. (2021). Antibacterial properties of thalloid liverworts Marchantia polymorpha L., Conocephalum conicum (L.) Dum. and Pellia endiviifolia (Dicks.) Dumort. in Journal of the Serbian Chemical Society
Serbian Chemical Society.(12), 1249-1258.
https://doi.org/10.2298/JSC210728084I

Ivković I, Bukvički D, Novaković M, Ivanović S, Stanojević O, Nikolić I, Veljić M. Antibacterial properties of thalloid liverworts Marchantia polymorpha L., Conocephalum conicum (L.) Dum. and Pellia endiviifolia (Dicks.) Dumort. in Journal of the Serbian Chemical Society. 2021;(12):1249-1258.
doi:10.2298/JSC210728084I .

Ivković, Ivana, Bukvički, Danka, Novaković, Miroslav, Ivanović, Stefan, Stanojević, Olja, Nikolić, Ivan, Veljić, Milan, "Antibacterial properties of thalloid liverworts Marchantia polymorpha L., Conocephalum conicum (L.) Dum. and Pellia endiviifolia (Dicks.) Dumort" in Journal of the Serbian Chemical Society, no. 12 (2021):1249-1258,
https://doi.org/10.2298/JSC210728084I . .

TY  - JOUR
AU  - Ivković, Ivana
AU  - Bukvicki, Danka
AU  - Novaković, Miroslav
AU  - Majstorović, Ivana
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Veljić, Milan
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4894
AB  - Liverworts are characterized by a high content of bioactive compounds reported to show antimicrobial, anticancer, and antioxidant properties. In this study, the biological effects of the methanol extract of the liverwort Pellia endiviifolia and its constituents, bis-bibenzyls perrottetin E, 10′-hydroxyperrottetin E, and 10,10′-dihydroxyperrottetin E, were investigated using human peripheral blood cells as a model system. The assessment of the investigated compounds comprised testing their genotoxicity, apoptotic potential, and redox modulating activities. The genotoxicity testing indicated that medium (25 µM) and high concentrations (100 µM) of the investigated compounds displayed genotoxic and antiproliferative effects in human lymphocytes as revealed by significant, concentration-dependent enhancement of the micronuclei incidence and decrease in the cytokinesis-block proliferation index compared to the control (P <.001). Analysis of leukocyte apoptosis showed a substantial potential of all investigated compounds to induce apoptosis, which was not concentration-dependent. The P endiviifolia extract and perrottetin E demonstrated considerable pro-apoptotic potential, even at the lowest concentration (1 µM) applied. Evaluation of the redox modulating effects, which comprised measuring erythrocyte catalase activity and the lymphocyte malondialdehyde level, showed that the investigated compounds did not induce oxidative stress in human peripheral blood cells (P >.05). The observed genotoxic, antiproliferative, and proapoptotic effects of the investigated compounds make them suitable for further comprehensive studies related to their possible applications as anticancer agents.
PB  - SAGE Publications Inc.
T2  - Natural Product Communications
T1  - Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro
VL  - 16
IS  - 11
SP  - 1
EP  - 9
DO  - 10.1177/1934578X211056422
ER  - 

@article{
author = "Ivković, Ivana and Bukvicki, Danka and Novaković, Miroslav and Majstorović, Ivana and Leskovac, Andreja and Petrović, Sandra and Veljić, Milan",
year = "2021",
abstract = "Liverworts are characterized by a high content of bioactive compounds reported to show antimicrobial, anticancer, and antioxidant properties. In this study, the biological effects of the methanol extract of the liverwort Pellia endiviifolia and its constituents, bis-bibenzyls perrottetin E, 10′-hydroxyperrottetin E, and 10,10′-dihydroxyperrottetin E, were investigated using human peripheral blood cells as a model system. The assessment of the investigated compounds comprised testing their genotoxicity, apoptotic potential, and redox modulating activities. The genotoxicity testing indicated that medium (25 µM) and high concentrations (100 µM) of the investigated compounds displayed genotoxic and antiproliferative effects in human lymphocytes as revealed by significant, concentration-dependent enhancement of the micronuclei incidence and decrease in the cytokinesis-block proliferation index compared to the control (P <.001). Analysis of leukocyte apoptosis showed a substantial potential of all investigated compounds to induce apoptosis, which was not concentration-dependent. The P endiviifolia extract and perrottetin E demonstrated considerable pro-apoptotic potential, even at the lowest concentration (1 µM) applied. Evaluation of the redox modulating effects, which comprised measuring erythrocyte catalase activity and the lymphocyte malondialdehyde level, showed that the investigated compounds did not induce oxidative stress in human peripheral blood cells (P >.05). The observed genotoxic, antiproliferative, and proapoptotic effects of the investigated compounds make them suitable for further comprehensive studies related to their possible applications as anticancer agents.",
publisher = "SAGE Publications Inc.",
journal = "Natural Product Communications",
title = "Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro",
volume = "16",
number = "11",
pages = "1-9",
doi = "10.1177/1934578X211056422"
}

Ivković, I., Bukvicki, D., Novaković, M., Majstorović, I., Leskovac, A., Petrović, S.,& Veljić, M.. (2021). Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro. in Natural Product Communications
SAGE Publications Inc.., 16(11), 1-9.
https://doi.org/10.1177/1934578X211056422

Ivković I, Bukvicki D, Novaković M, Majstorović I, Leskovac A, Petrović S, Veljić M. Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro. in Natural Product Communications. 2021;16(11):1-9.
doi:10.1177/1934578X211056422 .

Ivković, Ivana, Bukvicki, Danka, Novaković, Miroslav, Majstorović, Ivana, Leskovac, Andreja, Petrović, Sandra, Veljić, Milan, "Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro" in Natural Product Communications, 16, no. 11 (2021):1-9,
https://doi.org/10.1177/1934578X211056422 . .