TY  - JOUR
AU  - Makarov, Slobodan E.
AU  - Bodner, Michaela
AU  - Reineke, Doris
AU  - Vujisić, Ljubodrag V.
AU  - Todosijević, Marina
AU  - Antic, Dragan Z.
AU  - Vagalinski, Boyan
AU  - Lučić, Luka
AU  - Mitic, Bojan M.
AU  - Mitov, Plamen
AU  - Anđelković, Boban D.
AU  - Lucic, Sofija Pavkovic
AU  - Vajs, Vlatka
AU  - Tomić, Vladimir T.
AU  - Raspotnig, Guenther
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2735
AB  - Cave animals live under highly constant ecological conditions and in permanent darkness, and many evolutionary adaptations of cave-dwellers have been triggered by their specific environment. A similar "cave effect" leading to pronounced chemical interactions under such conditions may be assumed, but the chemoecology of troglobionts is mostly unknown. We investigated the defensive chemistry of a largely cave-dwelling julid group, the controversial tribe "Typhloiulini", and we included some cave-dwelling and some endogean representatives. While chemical defense in juliform diplopods is known to be highly uniform, and mainly based on methyl- and methoxy-substituted benzoquinones, the defensive secretions of typhloiulines contained ethyl-benzoquinones and related compounds. Interestingly, ethyl-benzoquinones were found in some, but not all cave-dwelling typhloiulines, and some non-cave dwellers also contained these compounds. On the other hand, ethyl-benzoquinones were not detected in troglobiont nor in endogean typhloiuline outgroups. In order to explain the taxonomic pattern of ethyl-benzoquinone occurrence, and to unravel whether a cave-effect triggered ethyl-benzoquinone evolution, we classed the "Typhloiulini" investigated here within a phylogenetic framework of julid taxa, and traced the evolutionary history of ethyl-benzoquinones in typhloiulines in relation to cave-dwelling. The results indicated a cave-independent evolution of ethyl-substituted benzoquinones, indicating the absence of a "cave effect" on the secretions of troglobiont Typhloiulini. Ethyl-benzoquinones probably evolved early in an epi- or endogean ancestor of a clade including several, but not all Typhloiulus (basically comprising a taxonomic entity known as "Typhloiulus sensu stricto") and Serboiulus. Ethyl-benzoquinones are proposed as novel and valuable chemical characters for julid systematics.
PB  - Springer, Dordrecht
T2  - Journal of Chemical Ecology
T1  - Chemical Ecology of Cave-Dwelling Millipedes: Defensive Secretions of the Typhloiulini (Diplopoda, Julida, Julidae)
VL  - 43
IS  - 4
SP  - 317
EP  - 326
DO  - 10.1007/s10886-017-0832-1
ER  - 

@article{
author = "Makarov, Slobodan E. and Bodner, Michaela and Reineke, Doris and Vujisić, Ljubodrag V. and Todosijević, Marina and Antic, Dragan Z. and Vagalinski, Boyan and Lučić, Luka and Mitic, Bojan M. and Mitov, Plamen and Anđelković, Boban D. and Lucic, Sofija Pavkovic and Vajs, Vlatka and Tomić, Vladimir T. and Raspotnig, Guenther",
year = "2017",
abstract = "Cave animals live under highly constant ecological conditions and in permanent darkness, and many evolutionary adaptations of cave-dwellers have been triggered by their specific environment. A similar "cave effect" leading to pronounced chemical interactions under such conditions may be assumed, but the chemoecology of troglobionts is mostly unknown. We investigated the defensive chemistry of a largely cave-dwelling julid group, the controversial tribe "Typhloiulini", and we included some cave-dwelling and some endogean representatives. While chemical defense in juliform diplopods is known to be highly uniform, and mainly based on methyl- and methoxy-substituted benzoquinones, the defensive secretions of typhloiulines contained ethyl-benzoquinones and related compounds. Interestingly, ethyl-benzoquinones were found in some, but not all cave-dwelling typhloiulines, and some non-cave dwellers also contained these compounds. On the other hand, ethyl-benzoquinones were not detected in troglobiont nor in endogean typhloiuline outgroups. In order to explain the taxonomic pattern of ethyl-benzoquinone occurrence, and to unravel whether a cave-effect triggered ethyl-benzoquinone evolution, we classed the "Typhloiulini" investigated here within a phylogenetic framework of julid taxa, and traced the evolutionary history of ethyl-benzoquinones in typhloiulines in relation to cave-dwelling. The results indicated a cave-independent evolution of ethyl-substituted benzoquinones, indicating the absence of a "cave effect" on the secretions of troglobiont Typhloiulini. Ethyl-benzoquinones probably evolved early in an epi- or endogean ancestor of a clade including several, but not all Typhloiulus (basically comprising a taxonomic entity known as "Typhloiulus sensu stricto") and Serboiulus. Ethyl-benzoquinones are proposed as novel and valuable chemical characters for julid systematics.",
publisher = "Springer, Dordrecht",
journal = "Journal of Chemical Ecology",
title = "Chemical Ecology of Cave-Dwelling Millipedes: Defensive Secretions of the Typhloiulini (Diplopoda, Julida, Julidae)",
volume = "43",
number = "4",
pages = "317-326",
doi = "10.1007/s10886-017-0832-1"
}

Makarov, S. E., Bodner, M., Reineke, D., Vujisić, L. V., Todosijević, M., Antic, D. Z., Vagalinski, B., Lučić, L., Mitic, B. M., Mitov, P., Anđelković, B. D., Lucic, S. P., Vajs, V., Tomić, V. T.,& Raspotnig, G.. (2017). Chemical Ecology of Cave-Dwelling Millipedes: Defensive Secretions of the Typhloiulini (Diplopoda, Julida, Julidae). in Journal of Chemical Ecology
Springer, Dordrecht., 43(4), 317-326.
https://doi.org/10.1007/s10886-017-0832-1

Makarov SE, Bodner M, Reineke D, Vujisić LV, Todosijević M, Antic DZ, Vagalinski B, Lučić L, Mitic BM, Mitov P, Anđelković BD, Lucic SP, Vajs V, Tomić VT, Raspotnig G. Chemical Ecology of Cave-Dwelling Millipedes: Defensive Secretions of the Typhloiulini (Diplopoda, Julida, Julidae). in Journal of Chemical Ecology. 2017;43(4):317-326.
doi:10.1007/s10886-017-0832-1 .

Makarov, Slobodan E., Bodner, Michaela, Reineke, Doris, Vujisić, Ljubodrag V., Todosijević, Marina, Antic, Dragan Z., Vagalinski, Boyan, Lučić, Luka, Mitic, Bojan M., Mitov, Plamen, Anđelković, Boban D., Lucic, Sofija Pavkovic, Vajs, Vlatka, Tomić, Vladimir T., Raspotnig, Guenther, "Chemical Ecology of Cave-Dwelling Millipedes: Defensive Secretions of the Typhloiulini (Diplopoda, Julida, Julidae)" in Journal of Chemical Ecology, 43, no. 4 (2017):317-326,
https://doi.org/10.1007/s10886-017-0832-1 . .

TY  - JOUR
AU  - Pavković-Lučić, Sofija
AU  - Todosijević, Marina
AU  - Savić, Tatjana
AU  - Vajs, Vlatka
AU  - Trajković, Jelena
AU  - Anđelković, Boban D.
AU  - Lučić, Luka
AU  - Krstić, Gordana
AU  - Makarov, Slobodan E.
AU  - Tomić, Vladimir T.
AU  - Miličić, Dragana
AU  - Vujisić, Ljubodrag V.
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2663
AB  - Cuticular hydrocarbons (CHCs) in Drosophila melanogaster represent the basis of chemical communication being involved in many important biological functions. The aim of this study was to characterize chemical composition and variation of cuticular profiles in five D. melanogaster strains. These strains were reared for approximately 300 generations on five diets: standard cornmeal medium and substrates prepared with apple, banana, tomato, and carrot. Differences in quantity and/or quality in CHCs were assumed as a result of activation of different metabolic pathways involved in food digestion and adaptations to the particular diet type. In total, independently of sex and strain, 66 chemical compounds were identified. In females of all strains, 60 compounds were identified, while, in males, 47 compounds were extracted. Certain new chemical compounds for D. melanogaster were found. MANOVA confirmed that CHC amounts significantly depend on sex and substrates, as well as on their interactions. Discriminant analysis revealed that flies belonging to apple' and carrot' strains exhibited the most noticeable differences in CHC repertoires. A non-hydrocarbon pheromone, cis-vaccenyl acetate (cVA) also contributed to the variation in the pheromone bouquet among the strains. Variability detected in CHCs and cVA may be used in the explanation of differences in mating behaviour previously determined in analyzed fly strains.
PB  - Wiley-VCH Verlag Gmbh, Weinheim
T2  - Chemistry and Biodiversity
T1  - "Does my Diet Affect my Perfume?' Identification and Quantification of Cuticular Compounds in Five Drosophila melanogaster Strains Maintained over 300 Generations on Different Diets
VL  - 13
IS  - 2
SP  - 224
EP  - 232
DO  - 10.1002/cbdv.201500064
ER  - 

@article{
author = "Pavković-Lučić, Sofija and Todosijević, Marina and Savić, Tatjana and Vajs, Vlatka and Trajković, Jelena and Anđelković, Boban D. and Lučić, Luka and Krstić, Gordana and Makarov, Slobodan E. and Tomić, Vladimir T. and Miličić, Dragana and Vujisić, Ljubodrag V.",
year = "2016",
abstract = "Cuticular hydrocarbons (CHCs) in Drosophila melanogaster represent the basis of chemical communication being involved in many important biological functions. The aim of this study was to characterize chemical composition and variation of cuticular profiles in five D. melanogaster strains. These strains were reared for approximately 300 generations on five diets: standard cornmeal medium and substrates prepared with apple, banana, tomato, and carrot. Differences in quantity and/or quality in CHCs were assumed as a result of activation of different metabolic pathways involved in food digestion and adaptations to the particular diet type. In total, independently of sex and strain, 66 chemical compounds were identified. In females of all strains, 60 compounds were identified, while, in males, 47 compounds were extracted. Certain new chemical compounds for D. melanogaster were found. MANOVA confirmed that CHC amounts significantly depend on sex and substrates, as well as on their interactions. Discriminant analysis revealed that flies belonging to apple' and carrot' strains exhibited the most noticeable differences in CHC repertoires. A non-hydrocarbon pheromone, cis-vaccenyl acetate (cVA) also contributed to the variation in the pheromone bouquet among the strains. Variability detected in CHCs and cVA may be used in the explanation of differences in mating behaviour previously determined in analyzed fly strains.",
publisher = "Wiley-VCH Verlag Gmbh, Weinheim",
journal = "Chemistry and Biodiversity",
title = ""Does my Diet Affect my Perfume?' Identification and Quantification of Cuticular Compounds in Five Drosophila melanogaster Strains Maintained over 300 Generations on Different Diets",
volume = "13",
number = "2",
pages = "224-232",
doi = "10.1002/cbdv.201500064"
}

Pavković-Lučić, S., Todosijević, M., Savić, T., Vajs, V., Trajković, J., Anđelković, B. D., Lučić, L., Krstić, G., Makarov, S. E., Tomić, V. T., Miličić, D.,& Vujisić, L. V.. (2016). "Does my Diet Affect my Perfume?' Identification and Quantification of Cuticular Compounds in Five Drosophila melanogaster Strains Maintained over 300 Generations on Different Diets. in Chemistry and Biodiversity
Wiley-VCH Verlag Gmbh, Weinheim., 13(2), 224-232.
https://doi.org/10.1002/cbdv.201500064

Pavković-Lučić S, Todosijević M, Savić T, Vajs V, Trajković J, Anđelković BD, Lučić L, Krstić G, Makarov SE, Tomić VT, Miličić D, Vujisić LV. "Does my Diet Affect my Perfume?' Identification and Quantification of Cuticular Compounds in Five Drosophila melanogaster Strains Maintained over 300 Generations on Different Diets. in Chemistry and Biodiversity. 2016;13(2):224-232.
doi:10.1002/cbdv.201500064 .

Pavković-Lučić, Sofija, Todosijević, Marina, Savić, Tatjana, Vajs, Vlatka, Trajković, Jelena, Anđelković, Boban D., Lučić, Luka, Krstić, Gordana, Makarov, Slobodan E., Tomić, Vladimir T., Miličić, Dragana, Vujisić, Ljubodrag V., ""Does my Diet Affect my Perfume?' Identification and Quantification of Cuticular Compounds in Five Drosophila melanogaster Strains Maintained over 300 Generations on Different Diets" in Chemistry and Biodiversity, 13, no. 2 (2016):224-232,
https://doi.org/10.1002/cbdv.201500064 . .

TY  - JOUR
AU  - Vujisić, Ljubodrag V.
AU  - Antic, Dragan Z.
AU  - Vučković, Ivan
AU  - Sekulic, Tatjana Lj.
AU  - Tomić, Vladimir T.
AU  - Mandić, Boris
AU  - Tešević, Vele
AU  - Curcic, Bozidar P. M.
AU  - Vajs, Vlatka
AU  - Makarov, Slobodan E.
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2733
AB  - The defensive secretions of two blaniulid millipedes, Nopoiulus kochii and Cibiniulus phlepsii, were characterized by GC-FID and GC/MS analyses, which showed the presence of a complex mixture of benzoquinones, hydroquinones, and oleates. Altogether, 13 compounds were identified. The major compound in the secretions of both analyzed species was 2-methyl-1,4-benzoquinone (toluquinone). The second major constituent in the N. kochii secretion was 2-methyl-3,4-(methylenedioxy)phenol, while in that of C. phlepsii, it was 2-methoxy-3-methyl-1,4-benzoquinone. The defensive secretion of N. kochii also showed a high content of hydroquinones (13.5%) in comparison to that of C. phlepsii (0.8%). Hexyl oleate and octyl oleate were detected for the first time in defensive millipede fluids. The chemical composition of the defensive secretions supports the chemotaxonomic position of the family Blaniulidae in the 'quinone' millipede clade.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry and Biodiversity
T1  - Chemical Defense in Millipedes (Myriapoda, Diplopoda): Do Representatives of the Family Blaniulidae Belong to the 'Quinone' Clade?
VL  - 11
IS  - 3
SP  - 483
EP  - 490
DO  - 10.1002/cbdv.201300355
ER  - 

@article{
author = "Vujisić, Ljubodrag V. and Antic, Dragan Z. and Vučković, Ivan and Sekulic, Tatjana Lj. and Tomić, Vladimir T. and Mandić, Boris and Tešević, Vele and Curcic, Bozidar P. M. and Vajs, Vlatka and Makarov, Slobodan E.",
year = "2014",
abstract = "The defensive secretions of two blaniulid millipedes, Nopoiulus kochii and Cibiniulus phlepsii, were characterized by GC-FID and GC/MS analyses, which showed the presence of a complex mixture of benzoquinones, hydroquinones, and oleates. Altogether, 13 compounds were identified. The major compound in the secretions of both analyzed species was 2-methyl-1,4-benzoquinone (toluquinone). The second major constituent in the N. kochii secretion was 2-methyl-3,4-(methylenedioxy)phenol, while in that of C. phlepsii, it was 2-methoxy-3-methyl-1,4-benzoquinone. The defensive secretion of N. kochii also showed a high content of hydroquinones (13.5%) in comparison to that of C. phlepsii (0.8%). Hexyl oleate and octyl oleate were detected for the first time in defensive millipede fluids. The chemical composition of the defensive secretions supports the chemotaxonomic position of the family Blaniulidae in the 'quinone' millipede clade.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry and Biodiversity",
title = "Chemical Defense in Millipedes (Myriapoda, Diplopoda): Do Representatives of the Family Blaniulidae Belong to the 'Quinone' Clade?",
volume = "11",
number = "3",
pages = "483-490",
doi = "10.1002/cbdv.201300355"
}

Vujisić, L. V., Antic, D. Z., Vučković, I., Sekulic, T. Lj., Tomić, V. T., Mandić, B., Tešević, V., Curcic, B. P. M., Vajs, V.,& Makarov, S. E.. (2014). Chemical Defense in Millipedes (Myriapoda, Diplopoda): Do Representatives of the Family Blaniulidae Belong to the 'Quinone' Clade?. in Chemistry and Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 11(3), 483-490.
https://doi.org/10.1002/cbdv.201300355

Vujisić LV, Antic DZ, Vučković I, Sekulic TL, Tomić VT, Mandić B, Tešević V, Curcic BPM, Vajs V, Makarov SE. Chemical Defense in Millipedes (Myriapoda, Diplopoda): Do Representatives of the Family Blaniulidae Belong to the 'Quinone' Clade?. in Chemistry and Biodiversity. 2014;11(3):483-490.
doi:10.1002/cbdv.201300355 .

Vujisić, Ljubodrag V., Antic, Dragan Z., Vučković, Ivan, Sekulic, Tatjana Lj., Tomić, Vladimir T., Mandić, Boris, Tešević, Vele, Curcic, Bozidar P. M., Vajs, Vlatka, Makarov, Slobodan E., "Chemical Defense in Millipedes (Myriapoda, Diplopoda): Do Representatives of the Family Blaniulidae Belong to the 'Quinone' Clade?" in Chemistry and Biodiversity, 11, no. 3 (2014):483-490,
https://doi.org/10.1002/cbdv.201300355 . .

TY  - JOUR
AU  - Sekulic, Tatjana Lj
AU  - Vujisić, Ljubodrag V.
AU  - Curcic, B. P. M.
AU  - Mandić, Boris
AU  - Antic, D. Z.
AU  - Trifunović, Snežana
AU  - Gođevac, Dejan
AU  - Vajs, Vlatka
AU  - Tomić, Vladimir T.
AU  - Makarov, Slobodan E.
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1522
AB  - A complex mixture of compounds was identified from the secretion of specimens of Unciger transsilvanicus. Phenol and p-cresol were detected for the first time in the family Julidae, and for the second time in the order Julida. Thirteen quinones were identified, with a great relative abundance of toloquinone and 2-methoxy-3-methyl- 1,4-benzoquinone. Hydroquinone was detected for the first time in the order Julida. Besides these compounds, isopentyl hexacosatetraenoate and isopentyl esters of saturated and unsaturated fatty acids with chain lengths from C-14 to C(20)were identified. The most abundant non-quinone compound was isopentyl eicosenoate. The relative abundance of quinone and non-quinone in the defensive fluid of U transsilvanicus was 77% and 23%, respectively. The phylogenetic importance of the registered compounds is briefly discussed.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of Biological Sciences
T1  - Quinones and non-quinones from the defensive secretion of unciger transsilvanicus (Verhoeff, 1899) (diplopoda, julida, julidae), from Serbia
VL  - 66
IS  - 1
SP  - 385
EP  - 391
DO  - 10.2298/ABS1401385S
ER  - 

@article{
author = "Sekulic, Tatjana Lj and Vujisić, Ljubodrag V. and Curcic, B. P. M. and Mandić, Boris and Antic, D. Z. and Trifunović, Snežana and Gođevac, Dejan and Vajs, Vlatka and Tomić, Vladimir T. and Makarov, Slobodan E.",
year = "2014",
abstract = "A complex mixture of compounds was identified from the secretion of specimens of Unciger transsilvanicus. Phenol and p-cresol were detected for the first time in the family Julidae, and for the second time in the order Julida. Thirteen quinones were identified, with a great relative abundance of toloquinone and 2-methoxy-3-methyl- 1,4-benzoquinone. Hydroquinone was detected for the first time in the order Julida. Besides these compounds, isopentyl hexacosatetraenoate and isopentyl esters of saturated and unsaturated fatty acids with chain lengths from C-14 to C(20)were identified. The most abundant non-quinone compound was isopentyl eicosenoate. The relative abundance of quinone and non-quinone in the defensive fluid of U transsilvanicus was 77% and 23%, respectively. The phylogenetic importance of the registered compounds is briefly discussed.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of Biological Sciences",
title = "Quinones and non-quinones from the defensive secretion of unciger transsilvanicus (Verhoeff, 1899) (diplopoda, julida, julidae), from Serbia",
volume = "66",
number = "1",
pages = "385-391",
doi = "10.2298/ABS1401385S"
}

Sekulic, T. L., Vujisić, L. V., Curcic, B. P. M., Mandić, B., Antic, D. Z., Trifunović, S., Gođevac, D., Vajs, V., Tomić, V. T.,& Makarov, S. E.. (2014). Quinones and non-quinones from the defensive secretion of unciger transsilvanicus (Verhoeff, 1899) (diplopoda, julida, julidae), from Serbia. in Archives of Biological Sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 66(1), 385-391.
https://doi.org/10.2298/ABS1401385S

Sekulic TL, Vujisić LV, Curcic BPM, Mandić B, Antic DZ, Trifunović S, Gođevac D, Vajs V, Tomić VT, Makarov SE. Quinones and non-quinones from the defensive secretion of unciger transsilvanicus (Verhoeff, 1899) (diplopoda, julida, julidae), from Serbia. in Archives of Biological Sciences. 2014;66(1):385-391.
doi:10.2298/ABS1401385S .

Sekulic, Tatjana Lj, Vujisić, Ljubodrag V., Curcic, B. P. M., Mandić, Boris, Antic, D. Z., Trifunović, Snežana, Gođevac, Dejan, Vajs, Vlatka, Tomić, Vladimir T., Makarov, Slobodan E., "Quinones and non-quinones from the defensive secretion of unciger transsilvanicus (Verhoeff, 1899) (diplopoda, julida, julidae), from Serbia" in Archives of Biological Sciences, 66, no. 1 (2014):385-391,
https://doi.org/10.2298/ABS1401385S . .