TY  - JOUR
AU  - Kekez, Branka
AU  - Gojgić-Cvijović, Gordana
AU  - Jakovljević, Dragica
AU  - Kojic, J R Stefanovic
AU  - Marković, Marijana
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1755
AB  - In this study, levan production by Bacillus licheniformis NS032 isolated from a petroleum sludge sample was investigated. High levan yield was obtained in a wide range of sucrose concentrations (up to 400 g/L) and, contrary to most levan-producing strains, using ammonium chloride as the sole N source. Interaction between sucrose, ammonium chloride, and initial pH of the medium in a low sucrose (60-200 g/L) and a high sucrose (300-400 g/L) system was analyzed by response surface methodology. According to the calculated model in the low sucrose system, maximum predicted levan yield was 47.8 g/L (sucrose 196.8 g/L, ammonium chloride 2.4 g/L, pH 7.0), while in the high sucrose system, levan yield was 99.2 g/L (sucrose 397.6 g/L, ammonium chloride 4.6 g/L, pH 7.4). In addition, protective effect of microbial levan against copper toxicity to Daphnia magna is observed for the first time. The acute toxicity (48 h EC50) of copper decreased from 0.14 to 0.44 mg/L by levan in concentration of 50 ppm.
PB  - Humana Press Inc, Totowa
T2  - Applied Biochemistry and Biotechnology
T1  - High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source
VL  - 175
IS  - 6
SP  - 3068
EP  - 3083
DO  - 10.1007/s12010-015-1475-8
ER  - 

@article{
author = "Kekez, Branka and Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Kojic, J R Stefanovic and Marković, Marijana and Beškoski, Vladimir and Vrvić, Miroslav",
year = "2015",
abstract = "In this study, levan production by Bacillus licheniformis NS032 isolated from a petroleum sludge sample was investigated. High levan yield was obtained in a wide range of sucrose concentrations (up to 400 g/L) and, contrary to most levan-producing strains, using ammonium chloride as the sole N source. Interaction between sucrose, ammonium chloride, and initial pH of the medium in a low sucrose (60-200 g/L) and a high sucrose (300-400 g/L) system was analyzed by response surface methodology. According to the calculated model in the low sucrose system, maximum predicted levan yield was 47.8 g/L (sucrose 196.8 g/L, ammonium chloride 2.4 g/L, pH 7.0), while in the high sucrose system, levan yield was 99.2 g/L (sucrose 397.6 g/L, ammonium chloride 4.6 g/L, pH 7.4). In addition, protective effect of microbial levan against copper toxicity to Daphnia magna is observed for the first time. The acute toxicity (48 h EC50) of copper decreased from 0.14 to 0.44 mg/L by levan in concentration of 50 ppm.",
publisher = "Humana Press Inc, Totowa",
journal = "Applied Biochemistry and Biotechnology",
title = "High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source",
volume = "175",
number = "6",
pages = "3068-3083",
doi = "10.1007/s12010-015-1475-8"
}

Kekez, B., Gojgić-Cvijović, G., Jakovljević, D., Kojic, J. R. S., Marković, M., Beškoski, V.,& Vrvić, M.. (2015). High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. in Applied Biochemistry and Biotechnology
Humana Press Inc, Totowa., 175(6), 3068-3083.
https://doi.org/10.1007/s12010-015-1475-8

Kekez B, Gojgić-Cvijović G, Jakovljević D, Kojic JRS, Marković M, Beškoski V, Vrvić M. High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source. in Applied Biochemistry and Biotechnology. 2015;175(6):3068-3083.
doi:10.1007/s12010-015-1475-8 .

Kekez, Branka, Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Kojic, J R Stefanovic, Marković, Marijana, Beškoski, Vladimir, Vrvić, Miroslav, "High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source" in Applied Biochemistry and Biotechnology, 175, no. 6 (2015):3068-3083,
https://doi.org/10.1007/s12010-015-1475-8 . .