TY  - JOUR
AU  - Milojkov, Dušan V.
AU  - Sokić, Miroslav D.
AU  - Živković-Radovanović, Vukosava
AU  - Manojlović, Vaso D.
AU  - Mutavdžić, Dragosav R.
AU  - Janjić, Goran
AU  - Radotić, Ksenija
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5804
AB  - In this study, luminescence and antibacterial behavior of pure fluorapatite (FAp) and praseodymium-nitrate-carbonate co-doped fluorapatite (PrNCFAp) nanopowders were investigated. Uniform nanopowders were synthesized by precipitation reaction followed by centrifugation and systematically characterized by XRD, FTIR, SEM–EDS, TG and PL methods. XRD analysis revealed the formation of hexagonal FAp crystals, and FTIR spectra indicate the presence of nitrate (NO3−) and carbonate (CO32−) species. SEM analyzes confirm agglomerates composed of irregular nanometer-sized spheres. Emission of FAp nanopowder occurred in the violet-blue region of the visible part of the spectrum, with redshift to the blue-green color region when Pr3+, NO3− and CO32− co-doped in the lattice. Analysis of luminescence spectra by MCR-ALS method extract three fluorophores from the PrNCFAp sample and showed simultaneous existents of emission-reabsorption-emission between dopants in FAp lattice. Antibacterial activity against pathogen Staphylococcus aureus was investigated before and after treatment of nanopowders by UVA radiation of 365 nm. Nanopowders irradiated with UVA compared to non-irradiated reduced Staphylococcus aureus by 84.9% for PrNCFAp and 33.3% for FAp in the first 0.5 h of contact, and 76.1% and 42.9% after 24 h of contact. In addition, the obtained luminescent nanomaterials showed a low degree of hemolytic activity and could potentially be candidates for further research in dentistry.
PB  - Springer
T2  - Optical and Quantum Electronics
T1  - Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions
VL  - 55
IS  - 1
SP  - 84
DO  - 10.1007/s11082-022-04347-7
ER  - 

@article{
author = "Milojkov, Dušan V. and Sokić, Miroslav D. and Živković-Radovanović, Vukosava and Manojlović, Vaso D. and Mutavdžić, Dragosav R. and Janjić, Goran and Radotić, Ksenija",
year = "2023",
abstract = "In this study, luminescence and antibacterial behavior of pure fluorapatite (FAp) and praseodymium-nitrate-carbonate co-doped fluorapatite (PrNCFAp) nanopowders were investigated. Uniform nanopowders were synthesized by precipitation reaction followed by centrifugation and systematically characterized by XRD, FTIR, SEM–EDS, TG and PL methods. XRD analysis revealed the formation of hexagonal FAp crystals, and FTIR spectra indicate the presence of nitrate (NO3−) and carbonate (CO32−) species. SEM analyzes confirm agglomerates composed of irregular nanometer-sized spheres. Emission of FAp nanopowder occurred in the violet-blue region of the visible part of the spectrum, with redshift to the blue-green color region when Pr3+, NO3− and CO32− co-doped in the lattice. Analysis of luminescence spectra by MCR-ALS method extract three fluorophores from the PrNCFAp sample and showed simultaneous existents of emission-reabsorption-emission between dopants in FAp lattice. Antibacterial activity against pathogen Staphylococcus aureus was investigated before and after treatment of nanopowders by UVA radiation of 365 nm. Nanopowders irradiated with UVA compared to non-irradiated reduced Staphylococcus aureus by 84.9% for PrNCFAp and 33.3% for FAp in the first 0.5 h of contact, and 76.1% and 42.9% after 24 h of contact. In addition, the obtained luminescent nanomaterials showed a low degree of hemolytic activity and could potentially be candidates for further research in dentistry.",
publisher = "Springer",
journal = "Optical and Quantum Electronics",
title = "Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions",
volume = "55",
number = "1",
pages = "84",
doi = "10.1007/s11082-022-04347-7"
}

Milojkov, D. V., Sokić, M. D., Živković-Radovanović, V., Manojlović, V. D., Mutavdžić, D. R., Janjić, G.,& Radotić, K.. (2023). Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions. in Optical and Quantum Electronics
Springer., 55(1), 84.
https://doi.org/10.1007/s11082-022-04347-7

Milojkov DV, Sokić MD, Živković-Radovanović V, Manojlović VD, Mutavdžić DR, Janjić G, Radotić K. Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions. in Optical and Quantum Electronics. 2023;55(1):84.
doi:10.1007/s11082-022-04347-7 .

Milojkov, Dušan V., Sokić, Miroslav D., Živković-Radovanović, Vukosava, Manojlović, Vaso D., Mutavdžić, Dragosav R., Janjić, Goran, Radotić, Ksenija, "Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions" in Optical and Quantum Electronics, 55, no. 1 (2023):84,
https://doi.org/10.1007/s11082-022-04347-7 . .

TY  - JOUR
AU  - Milojkov, Dušan V.
AU  - Silvestre, Oscar F.
AU  - Stanić, Vojislav Dj.
AU  - Janjić, Goran
AU  - Mutavdžić, Dragosav
AU  - Milanović, Marija
AU  - Nieder, Jana B.
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3275
AB  - Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.
PB  - Elsevier
T2  - Journal of Luminescence
T1  - Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents
VL  - 217
SP  - 116757
DO  - 10.1016/j.jlumin.2019.116757
ER  - 

@article{
author = "Milojkov, Dušan V. and Silvestre, Oscar F. and Stanić, Vojislav Dj. and Janjić, Goran and Mutavdžić, Dragosav and Milanović, Marija and Nieder, Jana B.",
year = "2020",
abstract = "Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.",
publisher = "Elsevier",
journal = "Journal of Luminescence",
title = "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents",
volume = "217",
pages = "116757",
doi = "10.1016/j.jlumin.2019.116757"
}

Milojkov, D. V., Silvestre, O. F., Stanić, V. Dj., Janjić, G., Mutavdžić, D., Milanović, M.,& Nieder, J. B.. (2020). Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence
Elsevier., 217, 116757.
https://doi.org/10.1016/j.jlumin.2019.116757

Milojkov DV, Silvestre OF, Stanić VD, Janjić G, Mutavdžić D, Milanović M, Nieder JB. Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence. 2020;217:116757.
doi:10.1016/j.jlumin.2019.116757 .

Milojkov, Dušan V., Silvestre, Oscar F., Stanić, Vojislav Dj., Janjić, Goran, Mutavdžić, Dragosav, Milanović, Marija, Nieder, Jana B., "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents" in Journal of Luminescence, 217 (2020):116757,
https://doi.org/10.1016/j.jlumin.2019.116757 . .

TY  - JOUR
AU  - Milojkov, Dušan V.
AU  - Silvestre, Oscar F.
AU  - Stanić, Vojislav Dj.
AU  - Janjić, Goran
AU  - Mutavdžić, Dragosav
AU  - Milanović, Marija
AU  - Nieder, Jana B.
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3276
AB  - Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.
PB  - Elsevier
T2  - Journal of Luminescence
T1  - Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents
VL  - 217
SP  - 116757
DO  - 10.1016/j.jlumin.2019.116757
ER  - 

@article{
author = "Milojkov, Dušan V. and Silvestre, Oscar F. and Stanić, Vojislav Dj. and Janjić, Goran and Mutavdžić, Dragosav and Milanović, Marija and Nieder, Jana B.",
year = "2020",
abstract = "Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.",
publisher = "Elsevier",
journal = "Journal of Luminescence",
title = "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents",
volume = "217",
pages = "116757",
doi = "10.1016/j.jlumin.2019.116757"
}

Milojkov, D. V., Silvestre, O. F., Stanić, V. Dj., Janjić, G., Mutavdžić, D., Milanović, M.,& Nieder, J. B.. (2020). Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence
Elsevier., 217, 116757.
https://doi.org/10.1016/j.jlumin.2019.116757

Milojkov DV, Silvestre OF, Stanić VD, Janjić G, Mutavdžić D, Milanović M, Nieder JB. Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence. 2020;217:116757.
doi:10.1016/j.jlumin.2019.116757 .

Milojkov, Dušan V., Silvestre, Oscar F., Stanić, Vojislav Dj., Janjić, Goran, Mutavdžić, Dragosav, Milanović, Marija, Nieder, Jana B., "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents" in Journal of Luminescence, 217 (2020):116757,
https://doi.org/10.1016/j.jlumin.2019.116757 . .