TY  - JOUR
AU  - Sentić, Milica
AU  - Sojic, Neso
AU  - Paolucci, Francesco
AU  - Arbault, Stéphane
AU  - Rapino, Stefania
AU  - Villani, Elena
AU  - Jović, Milica
AU  - Lesch, Andreas
AU  - Goudeau, Bertrand
AU  - Scarabino, Sabina
AU  - Giovanni, Valenti
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7132
AB  - We report here the development of coreactant-based electrogenerated chemiluminescence (ECL) as a surface-confined microscopy to image single cells and their membrane proteins. Labeling the entire cell membrane allows one to demonstrate that, by contrast with fluorescence, ECL emission is only detected from fluorophores located in the immediate vicinity of the electrode surface (i.e., 1–2 μm). Then, to present the potential diagnostic applications of our approach, we selected carbon nanotubes (CNT)-based inkjet-printed disposable electrodes for the direct ECL imaging of a labeled plasma receptor overexpressed on tumor cells. The ECL fluorophore was linked to an antibody and enabled to localize the ECL generation on the cancer cell membrane in close proximity to the electrode surface. Such a result is intrinsically associated with the unique ECL mechanism and is rationalized by considering the limited lifetimes of the electrogenerated coreactant radicals. The electrochemical stimulus used for luminescence generation does not suffer from background signals, such as the typical autofluorescence of biological samples. The presented surface-confined ECL microscopy should find promising applications in ultrasensitive single cell imaging assays.
PB  - American Chemical Society (ACS)
T2  - Journal of the American Chemical Society
T1  - Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis
VL  - 139
IS  - 46
SP  - 16830
EP  - 16837
DO  - 10.1021/jacs.7b09260
ER  - 

@article{
author = "Sentić, Milica and Sojic, Neso and Paolucci, Francesco and Arbault, Stéphane and Rapino, Stefania and Villani, Elena and Jović, Milica and Lesch, Andreas and Goudeau, Bertrand and Scarabino, Sabina and Giovanni, Valenti",
year = "2017",
abstract = "We report here the development of coreactant-based electrogenerated chemiluminescence (ECL) as a surface-confined microscopy to image single cells and their membrane proteins. Labeling the entire cell membrane allows one to demonstrate that, by contrast with fluorescence, ECL emission is only detected from fluorophores located in the immediate vicinity of the electrode surface (i.e., 1–2 μm). Then, to present the potential diagnostic applications of our approach, we selected carbon nanotubes (CNT)-based inkjet-printed disposable electrodes for the direct ECL imaging of a labeled plasma receptor overexpressed on tumor cells. The ECL fluorophore was linked to an antibody and enabled to localize the ECL generation on the cancer cell membrane in close proximity to the electrode surface. Such a result is intrinsically associated with the unique ECL mechanism and is rationalized by considering the limited lifetimes of the electrogenerated coreactant radicals. The electrochemical stimulus used for luminescence generation does not suffer from background signals, such as the typical autofluorescence of biological samples. The presented surface-confined ECL microscopy should find promising applications in ultrasensitive single cell imaging assays.",
publisher = "American Chemical Society (ACS)",
journal = "Journal of the American Chemical Society",
title = "Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis",
volume = "139",
number = "46",
pages = "16830-16837",
doi = "10.1021/jacs.7b09260"
}

Sentić, M., Sojic, N., Paolucci, F., Arbault, S., Rapino, S., Villani, E., Jović, M., Lesch, A., Goudeau, B., Scarabino, S.,& Giovanni, V.. (2017). Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis. in Journal of the American Chemical Society
American Chemical Society (ACS)., 139(46), 16830-16837.
https://doi.org/10.1021/jacs.7b09260

Sentić M, Sojic N, Paolucci F, Arbault S, Rapino S, Villani E, Jović M, Lesch A, Goudeau B, Scarabino S, Giovanni V. Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis. in Journal of the American Chemical Society. 2017;139(46):16830-16837.
doi:10.1021/jacs.7b09260 .

Sentić, Milica, Sojic, Neso, Paolucci, Francesco, Arbault, Stéphane, Rapino, Stefania, Villani, Elena, Jović, Milica, Lesch, Andreas, Goudeau, Bertrand, Scarabino, Sabina, Giovanni, Valenti, "Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis" in Journal of the American Chemical Society, 139, no. 46 (2017):16830-16837,
https://doi.org/10.1021/jacs.7b09260 . .