TY  - JOUR
AU  - Bjelogrlić, Snežana K.
AU  - Todorović, Tamara
AU  - Cvijetić, Ilija
AU  - Rodić, Marko V.
AU  - Vujčić, Miroslava
AU  - Marković, Sanja
AU  - Araskov, Jovana
AU  - Janović, Barbara
AU  - Emhemmed, Fathi
AU  - Muller, Christian D.
AU  - Filipovic, Nenad R.
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2493
AB  - A novel binuclear Cd complex (1) with hydrazone-based ligand was prepared and characterized by spectroscopy and single crystal X-ray diffraction techniques. Complex 1 reveals a strong pro-apoptotic activity in both human, mammary adenocarcinoma cells (MCF-7) and pancreatic AsPC-1 cancer stem cells (CSCs). While apoptosis undergoes mostly caspase-independent, 1 stimulates the activation of intrinsic pathway with noteworthy down regulation of caspase-8 activity in respect to non-treated controls. Distribution of cells over mitotic division indicates that 1 caused DNA damage in both cell lines, which is confirmed in DNA interaction studies. Compared to 1, cisplatin (CDDP) does not achieve cell death in 2D cultured AsPC-1 cells, while induces different pattern of cell cycle changes and caspase activation in 2D cultured MCF-7 cells, implying that these two compounds do not share similar mechanism of action. Additionally, 1 acts as a powerful inducer of mitochondrial superoxide production with dissipated trans-membrane potential in the majority of the treated cells already after 6 h of incubation. On 3D tumors, 1 displays a superior activity against CSC model, and at 100 M induces disintegration of spheroids within 2 days of incubation. Fluorescence spectroscopy, along with molecular docking show that compound 1 binds to the minor groove of DNA. Compound 1 binds to the human serum albumin (HSA) showing that the HSA can effectively transport and store 1 in the human body. Thus, our current study strongly supports further investigations on antitumor activity of 1 as a drug candidate for the treatment of highly resistant pancreatic cancer.
PB  - Elsevier Science Inc, New York
T2  - Journal of Inorganic Biochemistry
T1  - A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells
VL  - 190
SP  - 45
EP  - 66
DO  - 10.1016/j.jinorgbio.2018.10.002
ER  - 

@article{
author = "Bjelogrlić, Snežana K. and Todorović, Tamara and Cvijetić, Ilija and Rodić, Marko V. and Vujčić, Miroslava and Marković, Sanja and Araskov, Jovana and Janović, Barbara and Emhemmed, Fathi and Muller, Christian D. and Filipovic, Nenad R.",
year = "2019",
abstract = "A novel binuclear Cd complex (1) with hydrazone-based ligand was prepared and characterized by spectroscopy and single crystal X-ray diffraction techniques. Complex 1 reveals a strong pro-apoptotic activity in both human, mammary adenocarcinoma cells (MCF-7) and pancreatic AsPC-1 cancer stem cells (CSCs). While apoptosis undergoes mostly caspase-independent, 1 stimulates the activation of intrinsic pathway with noteworthy down regulation of caspase-8 activity in respect to non-treated controls. Distribution of cells over mitotic division indicates that 1 caused DNA damage in both cell lines, which is confirmed in DNA interaction studies. Compared to 1, cisplatin (CDDP) does not achieve cell death in 2D cultured AsPC-1 cells, while induces different pattern of cell cycle changes and caspase activation in 2D cultured MCF-7 cells, implying that these two compounds do not share similar mechanism of action. Additionally, 1 acts as a powerful inducer of mitochondrial superoxide production with dissipated trans-membrane potential in the majority of the treated cells already after 6 h of incubation. On 3D tumors, 1 displays a superior activity against CSC model, and at 100 M induces disintegration of spheroids within 2 days of incubation. Fluorescence spectroscopy, along with molecular docking show that compound 1 binds to the minor groove of DNA. Compound 1 binds to the human serum albumin (HSA) showing that the HSA can effectively transport and store 1 in the human body. Thus, our current study strongly supports further investigations on antitumor activity of 1 as a drug candidate for the treatment of highly resistant pancreatic cancer.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Inorganic Biochemistry",
title = "A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells",
volume = "190",
pages = "45-66",
doi = "10.1016/j.jinorgbio.2018.10.002"
}

Bjelogrlić, S. K., Todorović, T., Cvijetić, I., Rodić, M. V., Vujčić, M., Marković, S., Araskov, J., Janović, B., Emhemmed, F., Muller, C. D.,& Filipovic, N. R.. (2019). A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells. in Journal of Inorganic Biochemistry
Elsevier Science Inc, New York., 190, 45-66.
https://doi.org/10.1016/j.jinorgbio.2018.10.002

Bjelogrlić SK, Todorović T, Cvijetić I, Rodić MV, Vujčić M, Marković S, Araskov J, Janović B, Emhemmed F, Muller CD, Filipovic NR. A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells. in Journal of Inorganic Biochemistry. 2019;190:45-66.
doi:10.1016/j.jinorgbio.2018.10.002 .

Bjelogrlić, Snežana K., Todorović, Tamara, Cvijetić, Ilija, Rodić, Marko V., Vujčić, Miroslava, Marković, Sanja, Araskov, Jovana, Janović, Barbara, Emhemmed, Fathi, Muller, Christian D., Filipovic, Nenad R., "A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells" in Journal of Inorganic Biochemistry, 190 (2019):45-66,
https://doi.org/10.1016/j.jinorgbio.2018.10.002 . .

TY  - JOUR
AU  - Bjelogrlić, Snežana K.
AU  - Todorović, Tamara
AU  - Cvijetić, Ilija
AU  - Rodić, Marko V.
AU  - Vujčić, Miroslava
AU  - Marković, Sanja
AU  - Araskov, Jovana
AU  - Janović, Barbara
AU  - Emhemmed, Fathi
AU  - Muller, Christian D.
AU  - Filipović, Nenad R.
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2623
AB  - A novel binuclear Cd complex (1) with hydrazone-based ligand was prepared and characterized by spectroscopy and single crystal X-ray diffraction techniques. Complex 1 reveals a strong pro-apoptotic activity in both human, mammary adenocarcinoma cells (MCF-7) and pancreatic AsPC-1 cancer stem cells (CSCs). While apoptosis undergoes mostly caspase-independent, 1 stimulates the activation of intrinsic pathway with noteworthy down regulation of caspase-8 activity in respect to non-treated controls. Distribution of cells over mitotic division indicates that 1 caused DNA damage in both cell lines, which is confirmed in DNA interaction studies. Compared to 1, cisplatin (CDDP) does not achieve cell death in 2D cultured AsPC-1 cells, while induces different pattern of cell cycle changes and caspase activation in 2D cultured MCF-7 cells, implying that these two compounds do not share similar mechanism of action. Additionally, 1 acts as a powerful inducer of mitochondrial superoxide production with dissipated trans-membrane potential in the majority of the treated cells already after 6 h of incubation. On 3D tumors, 1 displays a superior activity against CSC model, and at 100 M induces disintegration of spheroids within 2 days of incubation. Fluorescence spectroscopy, along with molecular docking show that compound 1 binds to the minor groove of DNA. Compound 1 binds to the human serum albumin (HSA) showing that the HSA can effectively transport and store 1 in the human body. Thus, our current study strongly supports further investigations on antitumor activity of 1 as a drug candidate for the treatment of highly resistant pancreatic cancer.
PB  - Elsevier Science Inc, New York
T2  - Journal of Inorganic Biochemistry
T1  - A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells
VL  - 190
SP  - 45
EP  - 66
DO  - 10.1016/j.jinorgbio.2018.10.002
ER  - 

@article{
author = "Bjelogrlić, Snežana K. and Todorović, Tamara and Cvijetić, Ilija and Rodić, Marko V. and Vujčić, Miroslava and Marković, Sanja and Araskov, Jovana and Janović, Barbara and Emhemmed, Fathi and Muller, Christian D. and Filipović, Nenad R.",
year = "2019",
abstract = "A novel binuclear Cd complex (1) with hydrazone-based ligand was prepared and characterized by spectroscopy and single crystal X-ray diffraction techniques. Complex 1 reveals a strong pro-apoptotic activity in both human, mammary adenocarcinoma cells (MCF-7) and pancreatic AsPC-1 cancer stem cells (CSCs). While apoptosis undergoes mostly caspase-independent, 1 stimulates the activation of intrinsic pathway with noteworthy down regulation of caspase-8 activity in respect to non-treated controls. Distribution of cells over mitotic division indicates that 1 caused DNA damage in both cell lines, which is confirmed in DNA interaction studies. Compared to 1, cisplatin (CDDP) does not achieve cell death in 2D cultured AsPC-1 cells, while induces different pattern of cell cycle changes and caspase activation in 2D cultured MCF-7 cells, implying that these two compounds do not share similar mechanism of action. Additionally, 1 acts as a powerful inducer of mitochondrial superoxide production with dissipated trans-membrane potential in the majority of the treated cells already after 6 h of incubation. On 3D tumors, 1 displays a superior activity against CSC model, and at 100 M induces disintegration of spheroids within 2 days of incubation. Fluorescence spectroscopy, along with molecular docking show that compound 1 binds to the minor groove of DNA. Compound 1 binds to the human serum albumin (HSA) showing that the HSA can effectively transport and store 1 in the human body. Thus, our current study strongly supports further investigations on antitumor activity of 1 as a drug candidate for the treatment of highly resistant pancreatic cancer.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Inorganic Biochemistry",
title = "A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells",
volume = "190",
pages = "45-66",
doi = "10.1016/j.jinorgbio.2018.10.002"
}

Bjelogrlić, S. K., Todorović, T., Cvijetić, I., Rodić, M. V., Vujčić, M., Marković, S., Araskov, J., Janović, B., Emhemmed, F., Muller, C. D.,& Filipović, N. R.. (2019). A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells. in Journal of Inorganic Biochemistry
Elsevier Science Inc, New York., 190, 45-66.
https://doi.org/10.1016/j.jinorgbio.2018.10.002

Bjelogrlić SK, Todorović T, Cvijetić I, Rodić MV, Vujčić M, Marković S, Araskov J, Janović B, Emhemmed F, Muller CD, Filipović NR. A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells. in Journal of Inorganic Biochemistry. 2019;190:45-66.
doi:10.1016/j.jinorgbio.2018.10.002 .

Bjelogrlić, Snežana K., Todorović, Tamara, Cvijetić, Ilija, Rodić, Marko V., Vujčić, Miroslava, Marković, Sanja, Araskov, Jovana, Janović, Barbara, Emhemmed, Fathi, Muller, Christian D., Filipović, Nenad R., "A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells" in Journal of Inorganic Biochemistry, 190 (2019):45-66,
https://doi.org/10.1016/j.jinorgbio.2018.10.002 . .

TY  - CONF
AU  - Zizak, I. Besu
AU  - Zizak, B.
AU  - Janović, Barbara
AU  - Vujčić, Miroslava
AU  - Jokanović, Vukoman
AU  - Babić-Stojić, B.
AU  - Čolović, B.
AU  - Vujčić, Zoran
AU  - Stanojković, T.
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4673
AB  - Nanoparticles (NPs) are increasingly used in cancer
therapy as delivery agents and in the diagnosis of malignant diseases
as contrast agents for magnetic resonance imaging (MRI).
The aim of this work was in vitro assessments of Gd-NPs, Fe-
NPs, CoFe-NPs and Graphene Oxide-NPs cytotoxicity and genotoxicity
on some tumour and normal human cell lines.
PB  - Elsevier
C3  - ESMO Open
T1  - PO-410 Cytotoxicity and genotoxicity of new gadolinium, iron oxide, cobalt ferrite and graphene oxide nanoparticles on some tumour cell lines in vitro
VL  - 3
IS  - Suppl 2
SP  - A183
DO  - 10.1136/esmoopen-2018-EACR25.436
ER  - 

@conference{
author = "Zizak, I. Besu and Zizak, B. and Janović, Barbara and Vujčić, Miroslava and Jokanović, Vukoman and Babić-Stojić, B. and Čolović, B. and Vujčić, Zoran and Stanojković, T.",
year = "2018",
abstract = "Nanoparticles (NPs) are increasingly used in cancer
therapy as delivery agents and in the diagnosis of malignant diseases
as contrast agents for magnetic resonance imaging (MRI).
The aim of this work was in vitro assessments of Gd-NPs, Fe-
NPs, CoFe-NPs and Graphene Oxide-NPs cytotoxicity and genotoxicity
on some tumour and normal human cell lines.",
publisher = "Elsevier",
journal = "ESMO Open",
title = "PO-410 Cytotoxicity and genotoxicity of new gadolinium, iron oxide, cobalt ferrite and graphene oxide nanoparticles on some tumour cell lines in vitro",
volume = "3",
number = "Suppl 2",
pages = "A183",
doi = "10.1136/esmoopen-2018-EACR25.436"
}

Zizak, I. B., Zizak, B., Janović, B., Vujčić, M., Jokanović, V., Babić-Stojić, B., Čolović, B., Vujčić, Z.,& Stanojković, T.. (2018). PO-410 Cytotoxicity and genotoxicity of new gadolinium, iron oxide, cobalt ferrite and graphene oxide nanoparticles on some tumour cell lines in vitro. in ESMO Open
Elsevier., 3(Suppl 2), A183.
https://doi.org/10.1136/esmoopen-2018-EACR25.436

Zizak IB, Zizak B, Janović B, Vujčić M, Jokanović V, Babić-Stojić B, Čolović B, Vujčić Z, Stanojković T. PO-410 Cytotoxicity and genotoxicity of new gadolinium, iron oxide, cobalt ferrite and graphene oxide nanoparticles on some tumour cell lines in vitro. in ESMO Open. 2018;3(Suppl 2):A183.
doi:10.1136/esmoopen-2018-EACR25.436 .

Zizak, I. Besu, Zizak, B., Janović, Barbara, Vujčić, Miroslava, Jokanović, Vukoman, Babić-Stojić, B., Čolović, B., Vujčić, Zoran, Stanojković, T., "PO-410 Cytotoxicity and genotoxicity of new gadolinium, iron oxide, cobalt ferrite and graphene oxide nanoparticles on some tumour cell lines in vitro" in ESMO Open, 3, no. Suppl 2 (2018):A183,
https://doi.org/10.1136/esmoopen-2018-EACR25.436 . .

TY  - JOUR
AU  - Jakovljević, Katarina
AU  - Joksovic, Milan D.
AU  - Matić, Ivana Z.
AU  - Petrovic, Nina
AU  - Stanojković, Tatjana
AU  - Sladić, Dušan
AU  - Vujčić, Miroslava
AU  - Janović, Barbara
AU  - Joksovic, Ljubinka
AU  - Trifunović, Snežana
AU  - Markovic, Violeta
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2379
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3136
AB  - Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.
PB  - Royal Soc Chemistry, Cambridge
T2  - Medchemcomm
T1  - Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies
VL  - 9
IS  - 10
SP  - 1679
EP  - 1697
DO  - 10.1039/c8md00316e
ER  - 

@article{
author = "Jakovljević, Katarina and Joksovic, Milan D. and Matić, Ivana Z. and Petrovic, Nina and Stanojković, Tatjana and Sladić, Dušan and Vujčić, Miroslava and Janović, Barbara and Joksovic, Ljubinka and Trifunović, Snežana and Markovic, Violeta",
year = "2018",
abstract = "Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Medchemcomm",
title = "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies",
volume = "9",
number = "10",
pages = "1679-1697",
doi = "10.1039/c8md00316e"
}

Jakovljević, K., Joksovic, M. D., Matić, I. Z., Petrovic, N., Stanojković, T., Sladić, D., Vujčić, M., Janović, B., Joksovic, L., Trifunović, S.,& Markovic, V.. (2018). Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in Medchemcomm
Royal Soc Chemistry, Cambridge., 9(10), 1679-1697.
https://doi.org/10.1039/c8md00316e

Jakovljević K, Joksovic MD, Matić IZ, Petrovic N, Stanojković T, Sladić D, Vujčić M, Janović B, Joksovic L, Trifunović S, Markovic V. Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in Medchemcomm. 2018;9(10):1679-1697.
doi:10.1039/c8md00316e .

Jakovljević, Katarina, Joksovic, Milan D., Matić, Ivana Z., Petrovic, Nina, Stanojković, Tatjana, Sladić, Dušan, Vujčić, Miroslava, Janović, Barbara, Joksovic, Ljubinka, Trifunović, Snežana, Markovic, Violeta, "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies" in Medchemcomm, 9, no. 10 (2018):1679-1697,
https://doi.org/10.1039/c8md00316e . .

TY  - THES
AU  - Jožef, Barbara S.
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7670
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22847/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=23745033
UR  - https://nardus.mpn.gov.rs/handle/123456789/17609
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3740
AB  - Intenzivan industrijski razvoj propraćen je sve većom kompleksnošću sastava otpadnihvoda. Sintetičke boje su danas u širokoj primeni u velikom broju industrijskih grana. Većinaboja poseduje kompleksnu hemijsku strukturu, kao i povećanu hemijsku stabilnost što ih nakonotpuštanja u vodotokove čini ksenobioticima koji najčešće ispoljavaju i svoje rekalcitrantneosobine. Potreba za novim tehnološkim rešenjima radi uklanjanja širokog spektra ovihobojenih ksenobiotika dovela je do opsežnog istraživanja na ovom polju, dok je u poslednjihnekoliko godina istraživanje na polju primene enzima veoma uznapredovalo.Ova disertacija se bavi izolovanjem kiselih i baznih izoformi peroksidaze iz rena injihovom primenom u uklanjanju različitih obojenih ksenobiotika, optimizacijom reakcijeobezbojavanja, praćenjem i analizom proizvoda zaostalih nakon enzimske degradacije,sintezom imobilizata sa povećanom stabilnošću i efikasnošću u uklanjanju širokog spektraobojenih ksenobiotika. Cilj je bio razvijanje efikasne metode za uklanjanje i detoksifikacijuotpadne vode zaostale nakon procesa bojenja.Izolovanje i prečišćavanje kiselih, baznih i neutralnih izoformi peroksidaze iz renaurađeno je jonoizmenjivačkom hromatografijom na QAE-Sephadex koloni. Razdvojeneizoforme detektovane su zimogramskom detekcijom, nakon izoelektričnog fokusiranja.Optimizovani su uslovi reakcije obezbojavanja primenom rastvornih enzimskih preparata (0,14U mL-1 enzima i 0,44 mM vodonik-peroksida). Testirano je obezbojavanje 24 boje različitihstruktura i karakteristika u zavisnosti od pH i izoforme peroksidaze iz rena. Najveći stepenobezbojavanja postignut je u slučaju 17 boja sa primenom kisele izoforme peroksidaze iz rena:šest pripada klasi azo boja, tri boje triarilmetanskoj i jedna boja tiazinskoj klasi boja dok supreostalih 7 zaštićenih struktura nedostupnih za javnost. U slučaju 12 boja najboljeobezbojavanje postignuto je na pH 5, a u slučaju 10 boja na pH 9. Obezbojavanje je praćenoUV-Vis spektrofotometrijom, a degradacija je potvrđena analizom na HPLC-u.Obezbojavanjem model-boje (оranž II) kiselom izoformom peroksidaze iz rena dobijeniglavnih proizvodi obezbojavanja identifikovani su LC–ESI-ToF-MS tehnikom.Kisela izoforma peroksidaze iz rena uspešno je imobilizovana na 10 komercijalnodostupnih i laboratorijski sintetisanih nosača primenom različitih imobilizacionih tehnika.Kisela izoforma peroksidaze iz rena imobilizovana na hitozanu pokazala je najveću specifičnugvajakolnu aktivnost (2080 U g-1) kao i dekolorizacionu aktivnost uklanjanjem 175 mg L-1model boje. Dobijeni imobilizat pokazao je veću stabilnost prema vodonik-peroksidu upoređenju sa slobodnim enzimom. Imobilizat je pokazao i dobru operativnu stabilnost pa senjegova dekolorizaciona aktivnost nakon 7 ponovljenih ciklusa smanjila za samo 35%.Procena toksičnosti boja i proizvoda obezbojavanja ispitana je na Artemia salina.Obezbojavanje model-boja dovelo je do smanjenja % mortaliteta larvi A. salina u odnosu napočetni rastvor. Ekogenotoksičnost 8 model-boja testirana je na BEAS-2B ćelijama osnovnimi modifikovanim komet testom. Detektovani stepen oštećenja DNA iznosio je od 5 do 47%. Uslučaju model-boje, oranž II detektovana su oksidativna oštećenja pri izlaganju ćelija rastvoruboje (300 μg mL-1), dok izlaganje proizvodima degradacije nakon enzimskog tretmana sakiselom izoformom peroksidaze iz rena ne dovodi do istog efekta.Ispitane su interakcije boja i proizvoda degradacije sa DNA iz telećeg timusa iplazmidnom DNA na model boji oranž II i amido crno 10b primenom UV–Vis i fluorescentnespektrometrije i agaroznom elektroforezom. Nije uočeno značajno cepanje plazmidnogmolekula DNA nakon izlaganja rastvorima boja pre i nakon enzimskog obezbojavanja.
AB  - Intense industrial development has been accompanied by higher complexity ofeffluents. Synthetic dyes have been widely used in great number of industrial sectors.Majority of dyes possesses complex chemical structures, as well as chemical stability,which makes them persistent xenobiotics after their release in water bodies. The need fornew technological solutions for removal of these colored xenobiotics has led to majorongoing research on this field with big emphasis on enzyme application.Subject of this doctoral dissertation is isolation and application of acidic and basichorseradish peroxidase isoforms in decolorization of various colored xenobiotics,optimization of decolorization reactions, monitoring and analysis of decolorizationproducts, synthesis of immobilizates with higher stability and efficiency in removal ofwide spectrum of colored xenobiotics in order to develop efficient method for removaland detoxification of wastewater after the coloring process.Acidic isoforms have been purified from basic and neutral horseradish isoformsusing ion exchange chromatography on QAE-Sephadex column. Separated isoformshave been detected by zymograms after the isoelectric focusing. Decolorization reactionhas been optimized using soluble enzyme isoforms (0.14 U mL-1 and 0.44 mM hydrogenperoxide). Decolorization of 24 dyes of various structures and characteristics have beentested depending of pH and peroxidase isoforms. In case of 17 dyes highestdecolorization has been achieved using acidic horseradish peroxidase: six dyes are fromazo, three from triarylmethane and one from thiazine category while the rest of dyes havestructure which is unavailable to the public. Twelve dyes were decolorized best at pH 5,while 10 dyes were decolorized best at pH 9. Decolorization was monitored by UV–Visspectrometry, while degradation was confirmed by HPLC. By decolorization of modeldye (orange II) using acidic horseradish peroxidase the main decolorization productswere identified by LC–ESI-ToF-MS technique.Acidic isoform has been successfully immobilized on 10 commercially availableand laboratory synthetized carriers by various immobilization techniques. Acidichorseradish isoform immobilized on chitosan has shown highest specific activity towardguaiacol (2080 U g-1) as well as decolorization acvitity by removal of 175 mg L-1 ofmodel dye. The immobilizate obtained showed higher stability towards hydrogenperoxide in comparison with the soluble enzyme. The immobilizate has shown goodoperative stability since it has retained 65% of its decolorization activity after 7 repeatedcycles. The assessment of toxicity of dyes and decolorization products has been testedon Artemia salina. Decolorization of model dye has resulted in reduction of mortality ofA. salina larvae in comparison with initial dye. Ecogenotoxcitiy of 8 model dyes has beentested on BEAS-2B cells using basic and modified comet assay. DNA damage detectedwas from 5 to 47%. In case of model dye, orange II, oxidative DNA damage has beendetected after the exposure of cells to dye solutions (300 μg mL-1), while degradationproducts after enzymatic decolorization with acidic horseradish peroxidase showed noDNA damage.Interactions of dyes and degradation products with DNA from calf thymus andplasmid DNA has been studied using model dye orange II and amido black 10b by UV–Vis and fluorescent spectroscopy and agarose electrophoresis. Significant DNA strandbreaks on plasmid DNA has not been detected in either case.
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Primena rastvornih i imobilizovanih izoformi peroksidaze iz rena u uklanjanju obojenih ksenobiotika iz otpadne vode
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17609
ER  - 

@phdthesis{
author = "Jožef, Barbara S.",
year = "2018",
abstract = "Intenzivan industrijski razvoj propraćen je sve većom kompleksnošću sastava otpadnihvoda. Sintetičke boje su danas u širokoj primeni u velikom broju industrijskih grana. Većinaboja poseduje kompleksnu hemijsku strukturu, kao i povećanu hemijsku stabilnost što ih nakonotpuštanja u vodotokove čini ksenobioticima koji najčešće ispoljavaju i svoje rekalcitrantneosobine. Potreba za novim tehnološkim rešenjima radi uklanjanja širokog spektra ovihobojenih ksenobiotika dovela je do opsežnog istraživanja na ovom polju, dok je u poslednjihnekoliko godina istraživanje na polju primene enzima veoma uznapredovalo.Ova disertacija se bavi izolovanjem kiselih i baznih izoformi peroksidaze iz rena injihovom primenom u uklanjanju različitih obojenih ksenobiotika, optimizacijom reakcijeobezbojavanja, praćenjem i analizom proizvoda zaostalih nakon enzimske degradacije,sintezom imobilizata sa povećanom stabilnošću i efikasnošću u uklanjanju širokog spektraobojenih ksenobiotika. Cilj je bio razvijanje efikasne metode za uklanjanje i detoksifikacijuotpadne vode zaostale nakon procesa bojenja.Izolovanje i prečišćavanje kiselih, baznih i neutralnih izoformi peroksidaze iz renaurađeno je jonoizmenjivačkom hromatografijom na QAE-Sephadex koloni. Razdvojeneizoforme detektovane su zimogramskom detekcijom, nakon izoelektričnog fokusiranja.Optimizovani su uslovi reakcije obezbojavanja primenom rastvornih enzimskih preparata (0,14U mL-1 enzima i 0,44 mM vodonik-peroksida). Testirano je obezbojavanje 24 boje različitihstruktura i karakteristika u zavisnosti od pH i izoforme peroksidaze iz rena. Najveći stepenobezbojavanja postignut je u slučaju 17 boja sa primenom kisele izoforme peroksidaze iz rena:šest pripada klasi azo boja, tri boje triarilmetanskoj i jedna boja tiazinskoj klasi boja dok supreostalih 7 zaštićenih struktura nedostupnih za javnost. U slučaju 12 boja najboljeobezbojavanje postignuto je na pH 5, a u slučaju 10 boja na pH 9. Obezbojavanje je praćenoUV-Vis spektrofotometrijom, a degradacija je potvrđena analizom na HPLC-u.Obezbojavanjem model-boje (оranž II) kiselom izoformom peroksidaze iz rena dobijeniglavnih proizvodi obezbojavanja identifikovani su LC–ESI-ToF-MS tehnikom.Kisela izoforma peroksidaze iz rena uspešno je imobilizovana na 10 komercijalnodostupnih i laboratorijski sintetisanih nosača primenom različitih imobilizacionih tehnika.Kisela izoforma peroksidaze iz rena imobilizovana na hitozanu pokazala je najveću specifičnugvajakolnu aktivnost (2080 U g-1) kao i dekolorizacionu aktivnost uklanjanjem 175 mg L-1model boje. Dobijeni imobilizat pokazao je veću stabilnost prema vodonik-peroksidu upoređenju sa slobodnim enzimom. Imobilizat je pokazao i dobru operativnu stabilnost pa senjegova dekolorizaciona aktivnost nakon 7 ponovljenih ciklusa smanjila za samo 35%.Procena toksičnosti boja i proizvoda obezbojavanja ispitana je na Artemia salina.Obezbojavanje model-boja dovelo je do smanjenja % mortaliteta larvi A. salina u odnosu napočetni rastvor. Ekogenotoksičnost 8 model-boja testirana je na BEAS-2B ćelijama osnovnimi modifikovanim komet testom. Detektovani stepen oštećenja DNA iznosio je od 5 do 47%. Uslučaju model-boje, oranž II detektovana su oksidativna oštećenja pri izlaganju ćelija rastvoruboje (300 μg mL-1), dok izlaganje proizvodima degradacije nakon enzimskog tretmana sakiselom izoformom peroksidaze iz rena ne dovodi do istog efekta.Ispitane su interakcije boja i proizvoda degradacije sa DNA iz telećeg timusa iplazmidnom DNA na model boji oranž II i amido crno 10b primenom UV–Vis i fluorescentnespektrometrije i agaroznom elektroforezom. Nije uočeno značajno cepanje plazmidnogmolekula DNA nakon izlaganja rastvorima boja pre i nakon enzimskog obezbojavanja., Intense industrial development has been accompanied by higher complexity ofeffluents. Synthetic dyes have been widely used in great number of industrial sectors.Majority of dyes possesses complex chemical structures, as well as chemical stability,which makes them persistent xenobiotics after their release in water bodies. The need fornew technological solutions for removal of these colored xenobiotics has led to majorongoing research on this field with big emphasis on enzyme application.Subject of this doctoral dissertation is isolation and application of acidic and basichorseradish peroxidase isoforms in decolorization of various colored xenobiotics,optimization of decolorization reactions, monitoring and analysis of decolorizationproducts, synthesis of immobilizates with higher stability and efficiency in removal ofwide spectrum of colored xenobiotics in order to develop efficient method for removaland detoxification of wastewater after the coloring process.Acidic isoforms have been purified from basic and neutral horseradish isoformsusing ion exchange chromatography on QAE-Sephadex column. Separated isoformshave been detected by zymograms after the isoelectric focusing. Decolorization reactionhas been optimized using soluble enzyme isoforms (0.14 U mL-1 and 0.44 mM hydrogenperoxide). Decolorization of 24 dyes of various structures and characteristics have beentested depending of pH and peroxidase isoforms. In case of 17 dyes highestdecolorization has been achieved using acidic horseradish peroxidase: six dyes are fromazo, three from triarylmethane and one from thiazine category while the rest of dyes havestructure which is unavailable to the public. Twelve dyes were decolorized best at pH 5,while 10 dyes were decolorized best at pH 9. Decolorization was monitored by UV–Visspectrometry, while degradation was confirmed by HPLC. By decolorization of modeldye (orange II) using acidic horseradish peroxidase the main decolorization productswere identified by LC–ESI-ToF-MS technique.Acidic isoform has been successfully immobilized on 10 commercially availableand laboratory synthetized carriers by various immobilization techniques. Acidichorseradish isoform immobilized on chitosan has shown highest specific activity towardguaiacol (2080 U g-1) as well as decolorization acvitity by removal of 175 mg L-1 ofmodel dye. The immobilizate obtained showed higher stability towards hydrogenperoxide in comparison with the soluble enzyme. The immobilizate has shown goodoperative stability since it has retained 65% of its decolorization activity after 7 repeatedcycles. The assessment of toxicity of dyes and decolorization products has been testedon Artemia salina. Decolorization of model dye has resulted in reduction of mortality ofA. salina larvae in comparison with initial dye. Ecogenotoxcitiy of 8 model dyes has beentested on BEAS-2B cells using basic and modified comet assay. DNA damage detectedwas from 5 to 47%. In case of model dye, orange II, oxidative DNA damage has beendetected after the exposure of cells to dye solutions (300 μg mL-1), while degradationproducts after enzymatic decolorization with acidic horseradish peroxidase showed noDNA damage.Interactions of dyes and degradation products with DNA from calf thymus andplasmid DNA has been studied using model dye orange II and amido black 10b by UV–Vis and fluorescent spectroscopy and agarose electrophoresis. Significant DNA strandbreaks on plasmid DNA has not been detected in either case.",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Primena rastvornih i imobilizovanih izoformi peroksidaze iz rena u uklanjanju obojenih ksenobiotika iz otpadne vode",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17609"
}

Jožef, B. S.. (2018). Primena rastvornih i imobilizovanih izoformi peroksidaze iz rena u uklanjanju obojenih ksenobiotika iz otpadne vode. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_17609

Jožef BS. Primena rastvornih i imobilizovanih izoformi peroksidaze iz rena u uklanjanju obojenih ksenobiotika iz otpadne vode. in Универзитет у Београду. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_17609 .

Jožef, Barbara S., "Primena rastvornih i imobilizovanih izoformi peroksidaze iz rena u uklanjanju obojenih ksenobiotika iz otpadne vode" in Универзитет у Београду (2018),
https://hdl.handle.net/21.15107/rcub_nardus_17609 .

TY  - DATA
AU  - Jakovljević, Katarina
AU  - Joksovic, Milan D.
AU  - Matić, Ivana Z.
AU  - Petrovic, Nina
AU  - Stanojković, Tatjana
AU  - Sladić, Dušan
AU  - Vujčić, Miroslava
AU  - Janović, Barbara
AU  - Joksovic, Ljubinka
AU  - Trifunović, Snežana
AU  - Markovic, Violeta
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4534
AB  - Copies of 1H and 13C NMR spectra for 5a-m
PB  - Royal Society of Chemistry, Cambridge
T2  - Medchemcomm
T1  - Supplementary Information for: "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies"
UR  - https://hdl.handle.net/21.15107/rcub_cer_4534
ER  - 

@misc{
author = "Jakovljević, Katarina and Joksovic, Milan D. and Matić, Ivana Z. and Petrovic, Nina and Stanojković, Tatjana and Sladić, Dušan and Vujčić, Miroslava and Janović, Barbara and Joksovic, Ljubinka and Trifunović, Snežana and Markovic, Violeta",
year = "2018",
abstract = "Copies of 1H and 13C NMR spectra for 5a-m",
publisher = "Royal Society of Chemistry, Cambridge",
journal = "Medchemcomm",
title = "Supplementary Information for: "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies"",
url = "https://hdl.handle.net/21.15107/rcub_cer_4534"
}

Jakovljević, K., Joksovic, M. D., Matić, I. Z., Petrovic, N., Stanojković, T., Sladić, D., Vujčić, M., Janović, B., Joksovic, L., Trifunović, S.,& Markovic, V.. (2018). Supplementary Information for: "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies". in Medchemcomm
Royal Society of Chemistry, Cambridge..
https://hdl.handle.net/21.15107/rcub_cer_4534

Jakovljević K, Joksovic MD, Matić IZ, Petrovic N, Stanojković T, Sladić D, Vujčić M, Janović B, Joksovic L, Trifunović S, Markovic V. Supplementary Information for: "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies". in Medchemcomm. 2018;.
https://hdl.handle.net/21.15107/rcub_cer_4534 .

Jakovljević, Katarina, Joksovic, Milan D., Matić, Ivana Z., Petrovic, Nina, Stanojković, Tatjana, Sladić, Dušan, Vujčić, Miroslava, Janović, Barbara, Joksovic, Ljubinka, Trifunović, Snežana, Markovic, Violeta, "Supplementary Information for: "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies"" in Medchemcomm (2018),
https://hdl.handle.net/21.15107/rcub_cer_4534 .

TY  - JOUR
AU  - Jakovljević, Katarina
AU  - Joksovic, Milan D.
AU  - Matić, Ivana Z.
AU  - Petrovic, Nina
AU  - Stanojković, Tatjana
AU  - Sladić, Dušan
AU  - Vujčić, Miroslava
AU  - Janović, Barbara
AU  - Joksovic, Ljubinka
AU  - Trifunović, Snežana
AU  - Markovic, Violeta
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2379
AB  - Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.
PB  - Royal Soc Chemistry, Cambridge
T2  - Medchemcomm
T1  - Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies
VL  - 9
IS  - 10
SP  - 1679
EP  - 1697
DO  - 10.1039/c8md00316e
ER  - 

@article{
author = "Jakovljević, Katarina and Joksovic, Milan D. and Matić, Ivana Z. and Petrovic, Nina and Stanojković, Tatjana and Sladić, Dušan and Vujčić, Miroslava and Janović, Barbara and Joksovic, Ljubinka and Trifunović, Snežana and Markovic, Violeta",
year = "2018",
abstract = "Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Medchemcomm",
title = "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies",
volume = "9",
number = "10",
pages = "1679-1697",
doi = "10.1039/c8md00316e"
}

Jakovljević, K., Joksovic, M. D., Matić, I. Z., Petrovic, N., Stanojković, T., Sladić, D., Vujčić, M., Janović, B., Joksovic, L., Trifunović, S.,& Markovic, V.. (2018). Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in Medchemcomm
Royal Soc Chemistry, Cambridge., 9(10), 1679-1697.
https://doi.org/10.1039/c8md00316e

Jakovljević K, Joksovic MD, Matić IZ, Petrovic N, Stanojković T, Sladić D, Vujčić M, Janović B, Joksovic L, Trifunović S, Markovic V. Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in Medchemcomm. 2018;9(10):1679-1697.
doi:10.1039/c8md00316e .

Jakovljević, Katarina, Joksovic, Milan D., Matić, Ivana Z., Petrovic, Nina, Stanojković, Tatjana, Sladić, Dušan, Vujčić, Miroslava, Janović, Barbara, Joksovic, Ljubinka, Trifunović, Snežana, Markovic, Violeta, "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies" in Medchemcomm, 9, no. 10 (2018):1679-1697,
https://doi.org/10.1039/c8md00316e . .

TY  - CONF
AU  - Zizak, Z.
AU  - Janović, Barbara
AU  - Zizak, I.Besu
AU  - Vujčić, Z.
AU  - Stanković, Vesna
AU  - Matić, I.
AU  - Nikitović, M.
AU  - Stanojković, T.
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4674
AB  - A high cellular radiosensitivity is connected with a risk for development of severe side effects after radiotherapy. In this study we have attempted to find a correlation between the initial radiosensitivity of in vitro irradiated peripheral blood mononuclear cells (PBMC) of prostate cancer patients and the adverse side effects of radiotherapy.
PB  - Elsevier
C3  - ESMO Open
T1  - PO-129 In vitro radiosensitivity and repair kinetics of PBMCs from prostate cancer patients and healthy donors evaluated by comet assay
VL  - 3
IS  - Sup.2
SP  - A276
EP  - A277
DO  - 10.1136/esmoopen-2018-EACR25.653
ER  - 

@conference{
author = "Zizak, Z. and Janović, Barbara and Zizak, I.Besu and Vujčić, Z. and Stanković, Vesna and Matić, I. and Nikitović, M. and Stanojković, T.",
year = "2018",
abstract = "A high cellular radiosensitivity is connected with a risk for development of severe side effects after radiotherapy. In this study we have attempted to find a correlation between the initial radiosensitivity of in vitro irradiated peripheral blood mononuclear cells (PBMC) of prostate cancer patients and the adverse side effects of radiotherapy.",
publisher = "Elsevier",
journal = "ESMO Open",
title = "PO-129 In vitro radiosensitivity and repair kinetics of PBMCs from prostate cancer patients and healthy donors evaluated by comet assay",
volume = "3",
number = "Sup.2",
pages = "A276-A277",
doi = "10.1136/esmoopen-2018-EACR25.653"
}

Zizak, Z., Janović, B., Zizak, I.Besu, Vujčić, Z., Stanković, V., Matić, I., Nikitović, M.,& Stanojković, T.. (2018). PO-129 In vitro radiosensitivity and repair kinetics of PBMCs from prostate cancer patients and healthy donors evaluated by comet assay. in ESMO Open
Elsevier., 3(Sup.2), A276-A277.
https://doi.org/10.1136/esmoopen-2018-EACR25.653

Zizak Z, Janović B, Zizak I, Vujčić Z, Stanković V, Matić I, Nikitović M, Stanojković T. PO-129 In vitro radiosensitivity and repair kinetics of PBMCs from prostate cancer patients and healthy donors evaluated by comet assay. in ESMO Open. 2018;3(Sup.2):A276-A277.
doi:10.1136/esmoopen-2018-EACR25.653 .

Zizak, Z., Janović, Barbara, Zizak, I.Besu, Vujčić, Z., Stanković, Vesna, Matić, I., Nikitović, M., Stanojković, T., "PO-129 In vitro radiosensitivity and repair kinetics of PBMCs from prostate cancer patients and healthy donors evaluated by comet assay" in ESMO Open, 3, no. Sup.2 (2018):A276-A277,
https://doi.org/10.1136/esmoopen-2018-EACR25.653 . .

TY  - JOUR
AU  - Janović, Barbara
AU  - Collins, Andrew R.
AU  - Vujčić, Zoran
AU  - Vujčić, Miroslava
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3045
AB  - The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential.
PB  - Elsevier
T2  - Journal of Hazardous Materials
T1  - Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes
VL  - 321
SP  - 576
EP  - 585
DO  - 10.1016/j.jhazmat.2016.09.037
ER  - 

@article{
author = "Janović, Barbara and Collins, Andrew R. and Vujčić, Zoran and Vujčić, Miroslava",
year = "2017",
abstract = "The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential.",
publisher = "Elsevier",
journal = "Journal of Hazardous Materials",
title = "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes",
volume = "321",
pages = "576-585",
doi = "10.1016/j.jhazmat.2016.09.037"
}

Janović, B., Collins, A. R., Vujčić, Z.,& Vujčić, M.. (2017). Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. in Journal of Hazardous Materials
Elsevier., 321, 576-585.
https://doi.org/10.1016/j.jhazmat.2016.09.037

Janović B, Collins AR, Vujčić Z, Vujčić M. Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. in Journal of Hazardous Materials. 2017;321:576-585.
doi:10.1016/j.jhazmat.2016.09.037 .

Janović, Barbara, Collins, Andrew R., Vujčić, Zoran, Vujčić, Miroslava, "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes" in Journal of Hazardous Materials, 321 (2017):576-585,
https://doi.org/10.1016/j.jhazmat.2016.09.037 . .

TY  - JOUR
AU  - Janović, Barbara
AU  - Collins, Andrew R.
AU  - Vujčić, Zoran
AU  - Vujčić, Miroslava
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2070
AB  - The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential.
PB  - Elsevier
T2  - Journal of Hazardous Materials
T1  - Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes
VL  - 321
SP  - 576
EP  - 585
DO  - 10.1016/j.jhazmat.2016.09.037
ER  - 

@article{
author = "Janović, Barbara and Collins, Andrew R. and Vujčić, Zoran and Vujčić, Miroslava",
year = "2017",
abstract = "The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential.",
publisher = "Elsevier",
journal = "Journal of Hazardous Materials",
title = "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes",
volume = "321",
pages = "576-585",
doi = "10.1016/j.jhazmat.2016.09.037"
}

Janović, B., Collins, A. R., Vujčić, Z.,& Vujčić, M.. (2017). Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. in Journal of Hazardous Materials
Elsevier., 321, 576-585.
https://doi.org/10.1016/j.jhazmat.2016.09.037

Janović B, Collins AR, Vujčić Z, Vujčić M. Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. in Journal of Hazardous Materials. 2017;321:576-585.
doi:10.1016/j.jhazmat.2016.09.037 .

Janović, Barbara, Collins, Andrew R., Vujčić, Zoran, Vujčić, Miroslava, "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes" in Journal of Hazardous Materials, 321 (2017):576-585,
https://doi.org/10.1016/j.jhazmat.2016.09.037 . .

TY  - JOUR
AU  - Janović, Barbara
AU  - Vicovac, Milica Lj. Micic
AU  - Vujčić, Zoran
AU  - Vujčić, Miroslava
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2084
AB  - Peroxidases (EC 1.11.1.7) have enormous biotechnological applications. Usage of more abundant, basic isoforms of peroxidases in diagnostic kits and/or in immunochemistry has led to under exploitation and disregard of horseradish peroxidase (HRP) acidic isoforms. Therefore, acidic horseradish peroxidase (HRP-A) isoenzymewas used for the preparation of a biocatalyst with improved ability in dye decolorization. Ten biocatalysts were prepared by covalent binding of enzyme to chitosan and alginate, adsorption followed by cross-linking on inorganic support (aluminum oxide), and encapsulation in spherical calcium alginate beads via polyethylene glycol. Model dyes of 50 to 175 mg l(-1) were removed by the biocatalysts. Among the tested biocatalysts, the three with the highest specific activity and biodegradation rate were further studied (Chitosan-HRP, Al-GelHRP and Al-HRP-Gel). The impact of hydrogen peroxide concentration on dye decolorization was examined on the Chitosan-HRP biocatalyst, since the HRP is susceptible to inhibition/inactivation by high H2O2. On the other hand, H2O2 is needed as a co-substrate for the HRP, and the H2O2/dye ratio can greatly influence decolorization efficiency. Concentrations of H2O2 ranging from 0.22 to 4.4 mM showed no difference in terms of impact on the biocatalyst decolorization efficiency. The high decolorization efficiency of the biocatalysts was validated by the removal of 25 and 100 mg l(-1) anthraquinone (Remazol Brilliant Blue R (RBBR)), triphenylmethane (Coomassie Brilliant Blue CBB)), acridine (Acridine Orange (AO)), and formazan metal complex dye (Reactive Blue 52 (RB52)). After the seven consecutive decolorization cycles, the decolorization was still 53, 78, and 67% of the initial dye for the Al-HRP-Gel, Al-Gel-HRP, and Chitosan-HRP immobilizate, respectively. The results obtained showed potential of otherwise neglected acidic HRP isoforms as a cost-effective biocatalyst with significant potential in wastewater dyestuff treatment.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes
VL  - 24
IS  - 4
SP  - 3923
EP  - 3933
DO  - 10.1007/s11356-016-8100-4
ER  - 

@article{
author = "Janović, Barbara and Vicovac, Milica Lj. Micic and Vujčić, Zoran and Vujčić, Miroslava",
year = "2017",
abstract = "Peroxidases (EC 1.11.1.7) have enormous biotechnological applications. Usage of more abundant, basic isoforms of peroxidases in diagnostic kits and/or in immunochemistry has led to under exploitation and disregard of horseradish peroxidase (HRP) acidic isoforms. Therefore, acidic horseradish peroxidase (HRP-A) isoenzymewas used for the preparation of a biocatalyst with improved ability in dye decolorization. Ten biocatalysts were prepared by covalent binding of enzyme to chitosan and alginate, adsorption followed by cross-linking on inorganic support (aluminum oxide), and encapsulation in spherical calcium alginate beads via polyethylene glycol. Model dyes of 50 to 175 mg l(-1) were removed by the biocatalysts. Among the tested biocatalysts, the three with the highest specific activity and biodegradation rate were further studied (Chitosan-HRP, Al-GelHRP and Al-HRP-Gel). The impact of hydrogen peroxide concentration on dye decolorization was examined on the Chitosan-HRP biocatalyst, since the HRP is susceptible to inhibition/inactivation by high H2O2. On the other hand, H2O2 is needed as a co-substrate for the HRP, and the H2O2/dye ratio can greatly influence decolorization efficiency. Concentrations of H2O2 ranging from 0.22 to 4.4 mM showed no difference in terms of impact on the biocatalyst decolorization efficiency. The high decolorization efficiency of the biocatalysts was validated by the removal of 25 and 100 mg l(-1) anthraquinone (Remazol Brilliant Blue R (RBBR)), triphenylmethane (Coomassie Brilliant Blue CBB)), acridine (Acridine Orange (AO)), and formazan metal complex dye (Reactive Blue 52 (RB52)). After the seven consecutive decolorization cycles, the decolorization was still 53, 78, and 67% of the initial dye for the Al-HRP-Gel, Al-Gel-HRP, and Chitosan-HRP immobilizate, respectively. The results obtained showed potential of otherwise neglected acidic HRP isoforms as a cost-effective biocatalyst with significant potential in wastewater dyestuff treatment.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes",
volume = "24",
number = "4",
pages = "3923-3933",
doi = "10.1007/s11356-016-8100-4"
}

Janović, B., Vicovac, M. Lj. M., Vujčić, Z.,& Vujčić, M.. (2017). Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 24(4), 3923-3933.
https://doi.org/10.1007/s11356-016-8100-4

Janović B, Vicovac MLM, Vujčić Z, Vujčić M. Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes. in Environmental Science and Pollution Research. 2017;24(4):3923-3933.
doi:10.1007/s11356-016-8100-4 .

Janović, Barbara, Vicovac, Milica Lj. Micic, Vujčić, Zoran, Vujčić, Miroslava, "Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes" in Environmental Science and Pollution Research, 24, no. 4 (2017):3923-3933,
https://doi.org/10.1007/s11356-016-8100-4 . .

TY  - JOUR
AU  - Filipovic, Nenad R.
AU  - Bjelogrlić, Snežana K.
AU  - Todorović, Tamara
AU  - Blagojević, Vladimir A.
AU  - Muller, Christian D.
AU  - Marinković, Aleksandar D.
AU  - Vujčić, Miroslava
AU  - Janović, Barbara
AU  - Malešević, Aleksandar S.
AU  - Begovic, Nebojsa
AU  - Senćanski, Milan
AU  - Minić, Dragica M.
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2010
AB  - A new Ni(II) complex, [Ni(L)(H2O)] (1), with diethyl 3,3'-(2,2'-(1,1'-(pyridine-2,6-diyl) bis(ethan-1-yl-1-ylidene)) bis(hydrazin-1-yl-2-ylidene)) bis(3-oxopropanoate) ligand (H2L) was synthesized as a potential chemotherapeutic agent. Polidentate ligand was coordinated to Ni(II) NNN-tridentately, in dianionic form, while monodentate water coordination completed square-planar geometry around metal. Structure in the solution was determined by NMR spectroscopy and the same coordination mode was observed in the solid state using IR spectroscopy and further verified by DFT calculations and electrochemical studies. Thermal stability of 1 was determined in both air and nitrogen atmosphere. Anticancer activity of 1 was investigated on acute monocytic leukemia (THP-1) and pancreatic adenocarcinoma (AsPC-1) cell lines. On THP-1 cells 1 induced powerful apoptotic response (ED50 = 10 +/- 3 mu M), which was revealed to be only partially caspase-dependent, with activation of caspase-8 as the dominant course. This suggested that experimentally validated covalent binding of 1 to DNA is not the only mechanism responsible for programmed cell death. This was supported with experiments on AsPC-1 cells. Although treatment of those cells with 1 resulted in poor apoptotic response, cell cycle changes showed concentration-dependent shifts indicating a dual mechanism of activity. This study also reviews the results of preliminary biological screening, which demonstrates that 1 displays a unique pattern of anticancer activity with at least two mechanisms involved.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Ni(II) complex with bishydrazone ligand: synthesis, characterization, DNA binding studies and pro-apoptotic and pro-differentiation induction in human cancerous cell lines
VL  - 6
IS  - 110
SP  - 108726
EP  - 108740
DO  - 10.1039/c6ra24604d
ER  - 

@article{
author = "Filipovic, Nenad R. and Bjelogrlić, Snežana K. and Todorović, Tamara and Blagojević, Vladimir A. and Muller, Christian D. and Marinković, Aleksandar D. and Vujčić, Miroslava and Janović, Barbara and Malešević, Aleksandar S. and Begovic, Nebojsa and Senćanski, Milan and Minić, Dragica M.",
year = "2016",
abstract = "A new Ni(II) complex, [Ni(L)(H2O)] (1), with diethyl 3,3'-(2,2'-(1,1'-(pyridine-2,6-diyl) bis(ethan-1-yl-1-ylidene)) bis(hydrazin-1-yl-2-ylidene)) bis(3-oxopropanoate) ligand (H2L) was synthesized as a potential chemotherapeutic agent. Polidentate ligand was coordinated to Ni(II) NNN-tridentately, in dianionic form, while monodentate water coordination completed square-planar geometry around metal. Structure in the solution was determined by NMR spectroscopy and the same coordination mode was observed in the solid state using IR spectroscopy and further verified by DFT calculations and electrochemical studies. Thermal stability of 1 was determined in both air and nitrogen atmosphere. Anticancer activity of 1 was investigated on acute monocytic leukemia (THP-1) and pancreatic adenocarcinoma (AsPC-1) cell lines. On THP-1 cells 1 induced powerful apoptotic response (ED50 = 10 +/- 3 mu M), which was revealed to be only partially caspase-dependent, with activation of caspase-8 as the dominant course. This suggested that experimentally validated covalent binding of 1 to DNA is not the only mechanism responsible for programmed cell death. This was supported with experiments on AsPC-1 cells. Although treatment of those cells with 1 resulted in poor apoptotic response, cell cycle changes showed concentration-dependent shifts indicating a dual mechanism of activity. This study also reviews the results of preliminary biological screening, which demonstrates that 1 displays a unique pattern of anticancer activity with at least two mechanisms involved.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Ni(II) complex with bishydrazone ligand: synthesis, characterization, DNA binding studies and pro-apoptotic and pro-differentiation induction in human cancerous cell lines",
volume = "6",
number = "110",
pages = "108726-108740",
doi = "10.1039/c6ra24604d"
}

Filipovic, N. R., Bjelogrlić, S. K., Todorović, T., Blagojević, V. A., Muller, C. D., Marinković, A. D., Vujčić, M., Janović, B., Malešević, A. S., Begovic, N., Senćanski, M.,& Minić, D. M.. (2016). Ni(II) complex with bishydrazone ligand: synthesis, characterization, DNA binding studies and pro-apoptotic and pro-differentiation induction in human cancerous cell lines. in RSC Advances
Royal Soc Chemistry, Cambridge., 6(110), 108726-108740.
https://doi.org/10.1039/c6ra24604d

Filipovic NR, Bjelogrlić SK, Todorović T, Blagojević VA, Muller CD, Marinković AD, Vujčić M, Janović B, Malešević AS, Begovic N, Senćanski M, Minić DM. Ni(II) complex with bishydrazone ligand: synthesis, characterization, DNA binding studies and pro-apoptotic and pro-differentiation induction in human cancerous cell lines. in RSC Advances. 2016;6(110):108726-108740.
doi:10.1039/c6ra24604d .

Filipovic, Nenad R., Bjelogrlić, Snežana K., Todorović, Tamara, Blagojević, Vladimir A., Muller, Christian D., Marinković, Aleksandar D., Vujčić, Miroslava, Janović, Barbara, Malešević, Aleksandar S., Begovic, Nebojsa, Senćanski, Milan, Minić, Dragica M., "Ni(II) complex with bishydrazone ligand: synthesis, characterization, DNA binding studies and pro-apoptotic and pro-differentiation induction in human cancerous cell lines" in RSC Advances, 6, no. 110 (2016):108726-108740,
https://doi.org/10.1039/c6ra24604d . .

TY  - JOUR
AU  - Čobeljić, Božidar
AU  - Milenković, Milica R.
AU  - Pevec, Andrej
AU  - Turel, Iztok
AU  - Vujčić, Miroslava
AU  - Janović, Barbara
AU  - Gligorijević, Nevenka
AU  - Sladić, Dušan
AU  - Radulovic, Sinisa
AU  - Jovanovic, Katarina
AU  - Anđelković, Katarina
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2039
AB  - Square-planar azido Ni(II) complex with condensation product of 2-(diphenylphosphino)benzaldehyde and Girard's T reagent was synthesized and its crystal structure was determined. Cytotoxic activity of the azido complex and previously synthesized isothiocyanato, cyanato and chlorido Ni(II) complexes with this ligand was examined on six tumor cell lines (HeLa, A549, K562, MDA-MB-453, MDA-MB-361 and LS-174) and two normal cell line (MRC-5 and BEAS-2B). All the investigated nickel(II) complexes were cytotoxic against all tumor cell lines. The newly synthesized azido complex showed selectivity to HeLa and A549 tumor cell lines compared to the normal cells (for A549 IC50 was similar to that of cisplatin). Azido complex interferes with cell cycle phase distribution of A549 and HeLa cells and possesses nuclease activity towards supercoiled DNA. The observed selectivity of the azido complex for some tumor cell lines can be connected with its strong DNA damaging activity.
PB  - Springer, New York
T2  - Journal of Biological Inorganic Chemistry
T1  - Investigation of antitumor potential of Ni(II) complexes with tridentate PNO acylhydrazones of 2-(diphenylphosphino)benzaldehyde and monodentate pseudohalides
VL  - 21
IS  - 2
SP  - 145
EP  - 162
DO  - 10.1007/s00775-015-1315-x
ER  - 

@article{
author = "Čobeljić, Božidar and Milenković, Milica R. and Pevec, Andrej and Turel, Iztok and Vujčić, Miroslava and Janović, Barbara and Gligorijević, Nevenka and Sladić, Dušan and Radulovic, Sinisa and Jovanovic, Katarina and Anđelković, Katarina",
year = "2016",
abstract = "Square-planar azido Ni(II) complex with condensation product of 2-(diphenylphosphino)benzaldehyde and Girard's T reagent was synthesized and its crystal structure was determined. Cytotoxic activity of the azido complex and previously synthesized isothiocyanato, cyanato and chlorido Ni(II) complexes with this ligand was examined on six tumor cell lines (HeLa, A549, K562, MDA-MB-453, MDA-MB-361 and LS-174) and two normal cell line (MRC-5 and BEAS-2B). All the investigated nickel(II) complexes were cytotoxic against all tumor cell lines. The newly synthesized azido complex showed selectivity to HeLa and A549 tumor cell lines compared to the normal cells (for A549 IC50 was similar to that of cisplatin). Azido complex interferes with cell cycle phase distribution of A549 and HeLa cells and possesses nuclease activity towards supercoiled DNA. The observed selectivity of the azido complex for some tumor cell lines can be connected with its strong DNA damaging activity.",
publisher = "Springer, New York",
journal = "Journal of Biological Inorganic Chemistry",
title = "Investigation of antitumor potential of Ni(II) complexes with tridentate PNO acylhydrazones of 2-(diphenylphosphino)benzaldehyde and monodentate pseudohalides",
volume = "21",
number = "2",
pages = "145-162",
doi = "10.1007/s00775-015-1315-x"
}

Čobeljić, B., Milenković, M. R., Pevec, A., Turel, I., Vujčić, M., Janović, B., Gligorijević, N., Sladić, D., Radulovic, S., Jovanovic, K.,& Anđelković, K.. (2016). Investigation of antitumor potential of Ni(II) complexes with tridentate PNO acylhydrazones of 2-(diphenylphosphino)benzaldehyde and monodentate pseudohalides. in Journal of Biological Inorganic Chemistry
Springer, New York., 21(2), 145-162.
https://doi.org/10.1007/s00775-015-1315-x

Čobeljić B, Milenković MR, Pevec A, Turel I, Vujčić M, Janović B, Gligorijević N, Sladić D, Radulovic S, Jovanovic K, Anđelković K. Investigation of antitumor potential of Ni(II) complexes with tridentate PNO acylhydrazones of 2-(diphenylphosphino)benzaldehyde and monodentate pseudohalides. in Journal of Biological Inorganic Chemistry. 2016;21(2):145-162.
doi:10.1007/s00775-015-1315-x .

Čobeljić, Božidar, Milenković, Milica R., Pevec, Andrej, Turel, Iztok, Vujčić, Miroslava, Janović, Barbara, Gligorijević, Nevenka, Sladić, Dušan, Radulovic, Sinisa, Jovanovic, Katarina, Anđelković, Katarina, "Investigation of antitumor potential of Ni(II) complexes with tridentate PNO acylhydrazones of 2-(diphenylphosphino)benzaldehyde and monodentate pseudohalides" in Journal of Biological Inorganic Chemistry, 21, no. 2 (2016):145-162,
https://doi.org/10.1007/s00775-015-1315-x . .

TY  - CONF
AU  - Janović, Barbara
AU  - Vujčić, Zoran
AU  - Vujčić, Miroslava
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3550
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Sixth Conference, “Biochemistry and Interdisciplinarity: Transcending the Limits of Field”, Faculty of Chemistry, University of Belgrade, 18.11.2016. Belgrade, Serbia
T1  - Investigation of Vitamine C effects on DNA damage during enzymatic decolorization
SP  - 119
EP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_cer_3550
ER  - 

@conference{
author = "Janović, Barbara and Vujčić, Zoran and Vujčić, Miroslava",
year = "2016",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Sixth Conference, “Biochemistry and Interdisciplinarity: Transcending the Limits of Field”, Faculty of Chemistry, University of Belgrade, 18.11.2016. Belgrade, Serbia",
title = "Investigation of Vitamine C effects on DNA damage during enzymatic decolorization",
pages = "119-121",
url = "https://hdl.handle.net/21.15107/rcub_cer_3550"
}

Janović, B., Vujčić, Z.,& Vujčić, M.. (2016). Investigation of Vitamine C effects on DNA damage during enzymatic decolorization. in Serbian Biochemical Society Sixth Conference, “Biochemistry and Interdisciplinarity: Transcending the Limits of Field”, Faculty of Chemistry, University of Belgrade, 18.11.2016. Belgrade, Serbia
Serbian Biochemical Society., 119-121.
https://hdl.handle.net/21.15107/rcub_cer_3550

Janović B, Vujčić Z, Vujčić M. Investigation of Vitamine C effects on DNA damage during enzymatic decolorization. in Serbian Biochemical Society Sixth Conference, “Biochemistry and Interdisciplinarity: Transcending the Limits of Field”, Faculty of Chemistry, University of Belgrade, 18.11.2016. Belgrade, Serbia. 2016;:119-121.
https://hdl.handle.net/21.15107/rcub_cer_3550 .

Janović, Barbara, Vujčić, Zoran, Vujčić, Miroslava, "Investigation of Vitamine C effects on DNA damage during enzymatic decolorization" in Serbian Biochemical Society Sixth Conference, “Biochemistry and Interdisciplinarity: Transcending the Limits of Field”, Faculty of Chemistry, University of Belgrade, 18.11.2016. Belgrade, Serbia (2016):119-121,
https://hdl.handle.net/21.15107/rcub_cer_3550 .

TY  - JOUR
AU  - Vujčić, Zoran
AU  - Janović, Barbara
AU  - Lončar, Nikola
AU  - Margetić, Aleksandra
AU  - Božić, Nataša
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1760
AB  - Horseradish peroxidase (HRP) is enzyme first described more than 200 years ago and yet there are still some aspects of this potent enzyme to be tackled. Researchers were focused on most abundant isoenzyme HRP CIA while remaining, particularly anionic isoenzymes were discarded in purification process. This work describes exploitation of those isoenzymes for removal of recalcitrant pollutants such as reactive dyes. Results demonstrated that not only these enzymes can decolorize dyes but also in some cases anionic forms are more efficient than commercially produced cationic HRP form. Enzyme concentration of 0.14 U ml(-1) was found to provide maximum dye removal at optimized reaction conditions with dye concentration of 30 mg I-1. Majority of dyes tested were successfully decolorized at pH 5 or 7 while some dyes like Orange 2 and Reactive black 5 are decolorized most efficiently at pH 9. Anionic isoenzymes act by disrupting chromophore of Reactive black 5 while cationic HRP oxidize dye but leaves chromophore present.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Biodeterioration & Biodegradation
T1  - Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization
VL  - 97
SP  - 124
EP  - 127
DO  - 10.1016/j.ibiod.2014.10.007
ER  - 

@article{
author = "Vujčić, Zoran and Janović, Barbara and Lončar, Nikola and Margetić, Aleksandra and Božić, Nataša and Dojnov, Biljana and Vujčić, Miroslava",
year = "2015",
abstract = "Horseradish peroxidase (HRP) is enzyme first described more than 200 years ago and yet there are still some aspects of this potent enzyme to be tackled. Researchers were focused on most abundant isoenzyme HRP CIA while remaining, particularly anionic isoenzymes were discarded in purification process. This work describes exploitation of those isoenzymes for removal of recalcitrant pollutants such as reactive dyes. Results demonstrated that not only these enzymes can decolorize dyes but also in some cases anionic forms are more efficient than commercially produced cationic HRP form. Enzyme concentration of 0.14 U ml(-1) was found to provide maximum dye removal at optimized reaction conditions with dye concentration of 30 mg I-1. Majority of dyes tested were successfully decolorized at pH 5 or 7 while some dyes like Orange 2 and Reactive black 5 are decolorized most efficiently at pH 9. Anionic isoenzymes act by disrupting chromophore of Reactive black 5 while cationic HRP oxidize dye but leaves chromophore present.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Biodeterioration & Biodegradation",
title = "Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization",
volume = "97",
pages = "124-127",
doi = "10.1016/j.ibiod.2014.10.007"
}

Vujčić, Z., Janović, B., Lončar, N., Margetić, A., Božić, N., Dojnov, B.,& Vujčić, M.. (2015). Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization. in International Biodeterioration & Biodegradation
Elsevier Sci Ltd, Oxford., 97, 124-127.
https://doi.org/10.1016/j.ibiod.2014.10.007

Vujčić Z, Janović B, Lončar N, Margetić A, Božić N, Dojnov B, Vujčić M. Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization. in International Biodeterioration & Biodegradation. 2015;97:124-127.
doi:10.1016/j.ibiod.2014.10.007 .

Vujčić, Zoran, Janović, Barbara, Lončar, Nikola, Margetić, Aleksandra, Božić, Nataša, Dojnov, Biljana, Vujčić, Miroslava, "Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization" in International Biodeterioration & Biodegradation, 97 (2015):124-127,
https://doi.org/10.1016/j.ibiod.2014.10.007 . .

TY  - JOUR
AU  - Markovic, Violeta
AU  - Debeljak, Nevena
AU  - Stanojković, Tatjana
AU  - Kolundzija, Branka
AU  - Sladić, Dušan
AU  - Vujčić, Miroslava
AU  - Janović, Barbara
AU  - Tanic, Nikola
AU  - Perovic, Milka
AU  - Tesic, Vesna
AU  - Antic, Jadranka
AU  - Joksovic, Milan D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1745
AB  - Novel anthraquinone based chalcone compounds were synthesized starting from 1-acetylanthraquinone in a Claisen-Schmidt reaction and evaluated for their anticancer potential against three human cancer cell lines. Compounds 4a, 4b and 4j showed promising activity in inhibition of HeLa cells with IC50 values ranging from 2.36 to 2.73 mu M and low cytotoxicity against healthy MRC-5 cell lines. The effects that compounds produces on the cell cycle were investigated by flow cytometry. It was found that 4a, 4b and 4j cause the accumulation of cells in the S and G2/M phases in a dose-dependent manner and induce caspase-dependent apoptosis. All of three compounds exhibit calf thymus DNA-binding activity. The determined binding constants by absorption titrations (2.65 x 10(3) M-1, 1.36 x 10(3) M(-1)and 2.51 x 10(3) M-1 of 4a/CT-DNA, 4b/CT-DNA and 4j/CT-DNA, respectively) together with fluorescence displacement analysis designate 4a, 4b and 4j as strong minor groove binders, but no cleavage of plasmid DNA was observed.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - European Journal of Medicinal Chemistry
T1  - Anthraquinone-chalcone hybrids: Synthesis, preliminary antiproliferative evaluation and DNA-interaction studies
VL  - 89
SP  - 401
EP  - 410
DO  - 10.1016/j.ejmech.2014.10.055
ER  - 

@article{
author = "Markovic, Violeta and Debeljak, Nevena and Stanojković, Tatjana and Kolundzija, Branka and Sladić, Dušan and Vujčić, Miroslava and Janović, Barbara and Tanic, Nikola and Perovic, Milka and Tesic, Vesna and Antic, Jadranka and Joksovic, Milan D.",
year = "2015",
abstract = "Novel anthraquinone based chalcone compounds were synthesized starting from 1-acetylanthraquinone in a Claisen-Schmidt reaction and evaluated for their anticancer potential against three human cancer cell lines. Compounds 4a, 4b and 4j showed promising activity in inhibition of HeLa cells with IC50 values ranging from 2.36 to 2.73 mu M and low cytotoxicity against healthy MRC-5 cell lines. The effects that compounds produces on the cell cycle were investigated by flow cytometry. It was found that 4a, 4b and 4j cause the accumulation of cells in the S and G2/M phases in a dose-dependent manner and induce caspase-dependent apoptosis. All of three compounds exhibit calf thymus DNA-binding activity. The determined binding constants by absorption titrations (2.65 x 10(3) M-1, 1.36 x 10(3) M(-1)and 2.51 x 10(3) M-1 of 4a/CT-DNA, 4b/CT-DNA and 4j/CT-DNA, respectively) together with fluorescence displacement analysis designate 4a, 4b and 4j as strong minor groove binders, but no cleavage of plasmid DNA was observed.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "European Journal of Medicinal Chemistry",
title = "Anthraquinone-chalcone hybrids: Synthesis, preliminary antiproliferative evaluation and DNA-interaction studies",
volume = "89",
pages = "401-410",
doi = "10.1016/j.ejmech.2014.10.055"
}

Markovic, V., Debeljak, N., Stanojković, T., Kolundzija, B., Sladić, D., Vujčić, M., Janović, B., Tanic, N., Perovic, M., Tesic, V., Antic, J.,& Joksovic, M. D.. (2015). Anthraquinone-chalcone hybrids: Synthesis, preliminary antiproliferative evaluation and DNA-interaction studies. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 89, 401-410.
https://doi.org/10.1016/j.ejmech.2014.10.055

Markovic V, Debeljak N, Stanojković T, Kolundzija B, Sladić D, Vujčić M, Janović B, Tanic N, Perovic M, Tesic V, Antic J, Joksovic MD. Anthraquinone-chalcone hybrids: Synthesis, preliminary antiproliferative evaluation and DNA-interaction studies. in European Journal of Medicinal Chemistry. 2015;89:401-410.
doi:10.1016/j.ejmech.2014.10.055 .

Markovic, Violeta, Debeljak, Nevena, Stanojković, Tatjana, Kolundzija, Branka, Sladić, Dušan, Vujčić, Miroslava, Janović, Barbara, Tanic, Nikola, Perovic, Milka, Tesic, Vesna, Antic, Jadranka, Joksovic, Milan D., "Anthraquinone-chalcone hybrids: Synthesis, preliminary antiproliferative evaluation and DNA-interaction studies" in European Journal of Medicinal Chemistry, 89 (2015):401-410,
https://doi.org/10.1016/j.ejmech.2014.10.055 . .

TY  - JOUR
AU  - Markovic, Violeta
AU  - Janicijevic, Ana
AU  - Stanojković, Tatjana
AU  - Kolundzija, Branka
AU  - Sladić, Dušan
AU  - Vujčić, Miroslava
AU  - Janović, Barbara
AU  - Joksovic, Ljubinka
AU  - Djurdjevic, Predrag T.
AU  - Todorović, Nina
AU  - Trifunović, Snežana
AU  - Joksovic, Milan D.
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1343
AB  - A series of novel anthraquinone thiosemicarbazone derivatives in a tautomerizable keto-imine form was synthesized and tested for their in vitro cytotoxic activity against human cancer cells (HeLa, MDA-MB-361, MDA-MB-453, K562, A549) and human normal MRC-5 cells. Several compounds efficiently inhibited cancer cell growth at micromolar concentrations, especially against K562 and HeLa cells. As determined by flow cytometric analysis, anthraquinone thiosemicarbazone caused significant increase in the number of sub-G1 phase of HeLa cells and apoptosis in a concentration-dependent manner. Also, inhibition of caspase-3, -8, and -9 with specific caspase inhibitors reduced the apoptosis mediated by the tested compounds in HeLa cells. All anthraquinone-thiosemicarbazones exhibit calf thymus DNA-binding activity, but no cleavage of plasmid DNA was observed.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - European Journal of Medicinal Chemistry
T1  - Synthesis, cytotoxic activity and DNA-interaction studies of novel anthraquinone-thiosemicarbazones with tautomerizable methylene group
VL  - 64
SP  - 228
EP  - 238
DO  - 10.1016/j.ejmech.2013.03.071
ER  - 

@article{
author = "Markovic, Violeta and Janicijevic, Ana and Stanojković, Tatjana and Kolundzija, Branka and Sladić, Dušan and Vujčić, Miroslava and Janović, Barbara and Joksovic, Ljubinka and Djurdjevic, Predrag T. and Todorović, Nina and Trifunović, Snežana and Joksovic, Milan D.",
year = "2013",
abstract = "A series of novel anthraquinone thiosemicarbazone derivatives in a tautomerizable keto-imine form was synthesized and tested for their in vitro cytotoxic activity against human cancer cells (HeLa, MDA-MB-361, MDA-MB-453, K562, A549) and human normal MRC-5 cells. Several compounds efficiently inhibited cancer cell growth at micromolar concentrations, especially against K562 and HeLa cells. As determined by flow cytometric analysis, anthraquinone thiosemicarbazone caused significant increase in the number of sub-G1 phase of HeLa cells and apoptosis in a concentration-dependent manner. Also, inhibition of caspase-3, -8, and -9 with specific caspase inhibitors reduced the apoptosis mediated by the tested compounds in HeLa cells. All anthraquinone-thiosemicarbazones exhibit calf thymus DNA-binding activity, but no cleavage of plasmid DNA was observed.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "European Journal of Medicinal Chemistry",
title = "Synthesis, cytotoxic activity and DNA-interaction studies of novel anthraquinone-thiosemicarbazones with tautomerizable methylene group",
volume = "64",
pages = "228-238",
doi = "10.1016/j.ejmech.2013.03.071"
}

Markovic, V., Janicijevic, A., Stanojković, T., Kolundzija, B., Sladić, D., Vujčić, M., Janović, B., Joksovic, L., Djurdjevic, P. T., Todorović, N., Trifunović, S.,& Joksovic, M. D.. (2013). Synthesis, cytotoxic activity and DNA-interaction studies of novel anthraquinone-thiosemicarbazones with tautomerizable methylene group. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 64, 228-238.
https://doi.org/10.1016/j.ejmech.2013.03.071

Markovic V, Janicijevic A, Stanojković T, Kolundzija B, Sladić D, Vujčić M, Janović B, Joksovic L, Djurdjevic PT, Todorović N, Trifunović S, Joksovic MD. Synthesis, cytotoxic activity and DNA-interaction studies of novel anthraquinone-thiosemicarbazones with tautomerizable methylene group. in European Journal of Medicinal Chemistry. 2013;64:228-238.
doi:10.1016/j.ejmech.2013.03.071 .

Markovic, Violeta, Janicijevic, Ana, Stanojković, Tatjana, Kolundzija, Branka, Sladić, Dušan, Vujčić, Miroslava, Janović, Barbara, Joksovic, Ljubinka, Djurdjevic, Predrag T., Todorović, Nina, Trifunović, Snežana, Joksovic, Milan D., "Synthesis, cytotoxic activity and DNA-interaction studies of novel anthraquinone-thiosemicarbazones with tautomerizable methylene group" in European Journal of Medicinal Chemistry, 64 (2013):228-238,
https://doi.org/10.1016/j.ejmech.2013.03.071 . .

TY  - JOUR
AU  - Lončar, Nikola
AU  - Janović, Barbara
AU  - Vujčić, Miroslava
AU  - Vujčić, Zoran
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1115
AB  - Potatoes are desirable source for polyphenol oxidase (PPO, EC 1.14.18.1) purification because this enzyme can be purified from the food industry waste such are potato peels from potato chips industry. This paper presents data concerning decolorization of 7 different, so far untested textile dyes and 3 real samples (industry effluents) by a partially purified PPO. Under optimized conditions 93-99.9% removal of dyes was achieved after 1 h using 424-1700 U ml(-1) of PPO, depending on dye. Optimum pH for decolorization process of all dyes was found to be 3.0. Potato PPO was capable of removing reactive dyes and textile dye effluents without requiring any mediator. Decolorization was accomplished via insoluble polymers formations that were separated by filtration.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Biodeterioration & Biodegradation
T1  - Decolorization of textile dyes and effluents using potato (Solanum tuberosum) phenoloxidase
VL  - 72
SP  - 42
EP  - 45
DO  - 10.1016/j.ibiod.2012.05.001
ER  - 

@article{
author = "Lončar, Nikola and Janović, Barbara and Vujčić, Miroslava and Vujčić, Zoran",
year = "2012",
abstract = "Potatoes are desirable source for polyphenol oxidase (PPO, EC 1.14.18.1) purification because this enzyme can be purified from the food industry waste such are potato peels from potato chips industry. This paper presents data concerning decolorization of 7 different, so far untested textile dyes and 3 real samples (industry effluents) by a partially purified PPO. Under optimized conditions 93-99.9% removal of dyes was achieved after 1 h using 424-1700 U ml(-1) of PPO, depending on dye. Optimum pH for decolorization process of all dyes was found to be 3.0. Potato PPO was capable of removing reactive dyes and textile dye effluents without requiring any mediator. Decolorization was accomplished via insoluble polymers formations that were separated by filtration.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Biodeterioration & Biodegradation",
title = "Decolorization of textile dyes and effluents using potato (Solanum tuberosum) phenoloxidase",
volume = "72",
pages = "42-45",
doi = "10.1016/j.ibiod.2012.05.001"
}

Lončar, N., Janović, B., Vujčić, M.,& Vujčić, Z.. (2012). Decolorization of textile dyes and effluents using potato (Solanum tuberosum) phenoloxidase. in International Biodeterioration & Biodegradation
Elsevier Sci Ltd, Oxford., 72, 42-45.
https://doi.org/10.1016/j.ibiod.2012.05.001

Lončar N, Janović B, Vujčić M, Vujčić Z. Decolorization of textile dyes and effluents using potato (Solanum tuberosum) phenoloxidase. in International Biodeterioration & Biodegradation. 2012;72:42-45.
doi:10.1016/j.ibiod.2012.05.001 .

Lončar, Nikola, Janović, Barbara, Vujčić, Miroslava, Vujčić, Zoran, "Decolorization of textile dyes and effluents using potato (Solanum tuberosum) phenoloxidase" in International Biodeterioration & Biodegradation, 72 (2012):42-45,
https://doi.org/10.1016/j.ibiod.2012.05.001 . .