@conference{
author = "Ivanović, Stefan and Gođevac, Dejan and Simić, Katarina and Anđelković, Boban and Jovanović, Ž. D. and Rakić, Tamara",
year = "2022",
abstract = "Ramonda serbica and R. nathaliae are resurrection plants that have the
remarkable ability to survive the complete desiccation during periods of drought and
rapidly revive when rewatered and rehydrated. To investigate metabolic changes
during their desiccation and recovery process NMR-based metabolomics approach
coupled with multivariate data analysis was utilized to identify the metabolomes of
the plants from 90 biological replicates. The NMR metabolomics profiles of R.
serbica and R. nathaliae were subjected to multivariate data analysis. PCA was
performed, which resulted in eight principal components (PCs) in both models,
explaining 77.0% of the total data variance in the model with R. serbica samples,
and 79.5% of the variance in the model with R. nathaliae samples. Using NMR
experiments, the content of the two most dominant polar components found in the
leaves of these two plants was determined. Sucrose and the polyphenolic glycoside
myconoside were predominant in almost equal amounts in all samples studied,
regardless of their water content at sampling. Using of 1D and 2D NMR
experiments the main components have been successfully identified. Also, it was
necessary to isolate and purify the myconoside to confirm the structure.",
publisher = "Niš : Department of Biology and Ecology, Faculty of Science and Mathematics, Belgrade : Institute for Nature Conservation of Serbia",
journal = "Book of Abstracts - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 26th to 29th June, 2022, Kladovo",
title = "NMR metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and Ramonda nathaliae",
pages = "171-171",
url = "https://hdl.handle.net/21.15107/rcub_cer_5522"
}
