TY  - JOUR
AU  - Gođevac, Dejan
AU  - Ivanović, Stefan
AU  - Simić, Katarina
AU  - Anđelković, Boban
AU  - Jovanović, Živko
AU  - Rakić, Tamara
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5165
AB  - Introduction
Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated.

Objective
To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process

Methods
Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates.

Results
Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples.

Conclusion
Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.
PB  - Willey
T2  - Phytochemical Analysis
T1  - Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae
VL  - 33
IS  - 6
SP  - 961
SP  - 961
EP  - 970
DO  - 10.1002/pca.3151
ER  - 

@article{
author = "Gođevac, Dejan and Ivanović, Stefan and Simić, Katarina and Anđelković, Boban and Jovanović, Živko and Rakić, Tamara",
year = "2022",
abstract = "Introduction
Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated.

Objective
To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process

Methods
Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates.

Results
Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples.

Conclusion
Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.",
publisher = "Willey",
journal = "Phytochemical Analysis",
title = "Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae",
volume = "33",
number = "6",
pages = "961-961-970",
doi = "10.1002/pca.3151"
}

Gođevac, D., Ivanović, S., Simić, K., Anđelković, B., Jovanović, Ž.,& Rakić, T.. (2022). Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae. in Phytochemical Analysis
Willey., 33(6), 961-970.
https://doi.org/10.1002/pca.3151

Gođevac D, Ivanović S, Simić K, Anđelković B, Jovanović Ž, Rakić T. Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae. in Phytochemical Analysis. 2022;33(6):961-970.
doi:10.1002/pca.3151 .

Gođevac, Dejan, Ivanović, Stefan, Simić, Katarina, Anđelković, Boban, Jovanović, Živko, Rakić, Tamara, "Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae" in Phytochemical Analysis, 33, no. 6 (2022):961-970,
https://doi.org/10.1002/pca.3151 . .

TY  - CONF
AU  - Gođevac, Dejan
AU  - Simić, Katarina
AU  - Ivanović, Stefan
AU  - Anđelković, Boban
AU  - Jovanović, Živko
AU  - Rakić, Tamara
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5524
AB  - Ramonda serbica represents the remnant of the Tertiary tropical and subtropical flora in Europe and is the rare resurrection plants of Northern Hemisphere temperate zone. The plant remains well-hydrated during spring, late autumn and in winter. In summer and early autumn when plants are subjected to drought, their desiccation tolerance comes into operation and they spend it in anabiosis [1]. In this study, metabolic responses to dehydration and rehydration of R. serbica, were investigated. For this purpose, GC-MS/FID
based metabolomics method was performed. Leaves from the control (well-watered), dehydrated and partially rehydrated plants were sampled. Each leaf was powdered using liquid nitrogen and then freeze-dried. The internal standard (10-undecenoic acid) was added to the dry plant material and then extracted in methanol/water using ultra-sonication. After centrifugation of the mixture, the supernatant was dried and derivatized using two-step procedure involving oximation and silylation. GC-MS/FID analysis of each sample was then performed. The metabolites were identified using El-MS spectra. The areas from FID chromatograms were used for multivariate data analysis. Two OPLS-DA models were applied to investigate dehydration and rehydration process. According to high VIP scores, sugars, such as fructose, glucose, galactinol, sucrose, together with glyceric acid, xylonic acid 8-lactone and aspartic acid were found to be the most influential in the OPLS-DA models.
PB  - ICNPU-2019
C3  - Book of abstracts - 4th international conference on  natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria
T1  - GC-MS based metabolomics study of the resurrection plant Ramonda serbica
SP  - 189
EP  - 190
UR  - https://hdl.handle.net/21.15107/rcub_cer_5524
ER  - 

@conference{
author = "Gođevac, Dejan and Simić, Katarina and Ivanović, Stefan and Anđelković, Boban and Jovanović, Živko and Rakić, Tamara",
year = "2019",
abstract = "Ramonda serbica represents the remnant of the Tertiary tropical and subtropical flora in Europe and is the rare resurrection plants of Northern Hemisphere temperate zone. The plant remains well-hydrated during spring, late autumn and in winter. In summer and early autumn when plants are subjected to drought, their desiccation tolerance comes into operation and they spend it in anabiosis [1]. In this study, metabolic responses to dehydration and rehydration of R. serbica, were investigated. For this purpose, GC-MS/FID
based metabolomics method was performed. Leaves from the control (well-watered), dehydrated and partially rehydrated plants were sampled. Each leaf was powdered using liquid nitrogen and then freeze-dried. The internal standard (10-undecenoic acid) was added to the dry plant material and then extracted in methanol/water using ultra-sonication. After centrifugation of the mixture, the supernatant was dried and derivatized using two-step procedure involving oximation and silylation. GC-MS/FID analysis of each sample was then performed. The metabolites were identified using El-MS spectra. The areas from FID chromatograms were used for multivariate data analysis. Two OPLS-DA models were applied to investigate dehydration and rehydration process. According to high VIP scores, sugars, such as fructose, glucose, galactinol, sucrose, together with glyceric acid, xylonic acid 8-lactone and aspartic acid were found to be the most influential in the OPLS-DA models.",
publisher = "ICNPU-2019",
journal = "Book of abstracts - 4th international conference on  natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria",
title = "GC-MS based metabolomics study of the resurrection plant Ramonda serbica",
pages = "189-190",
url = "https://hdl.handle.net/21.15107/rcub_cer_5524"
}

Gođevac, D., Simić, K., Ivanović, S., Anđelković, B., Jovanović, Ž.,& Rakić, T.. (2019). GC-MS based metabolomics study of the resurrection plant Ramonda serbica. in Book of abstracts - 4th international conference on  natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria
ICNPU-2019., 189-190.
https://hdl.handle.net/21.15107/rcub_cer_5524

Gođevac D, Simić K, Ivanović S, Anđelković B, Jovanović Ž, Rakić T. GC-MS based metabolomics study of the resurrection plant Ramonda serbica. in Book of abstracts - 4th international conference on  natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria. 2019;:189-190.
https://hdl.handle.net/21.15107/rcub_cer_5524 .

Gođevac, Dejan, Simić, Katarina, Ivanović, Stefan, Anđelković, Boban, Jovanović, Živko, Rakić, Tamara, "GC-MS based metabolomics study of the resurrection plant Ramonda serbica" in Book of abstracts - 4th international conference on  natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria (2019):189-190,
https://hdl.handle.net/21.15107/rcub_cer_5524 .