TY  - JOUR
AU  - Mikavica, Ivana
AU  - Ranđelović, Dragana
AU  - Đorđević, Vladan
AU  - Rakić, Tamara
AU  - Gajić, Gordana
AU  - Mutić, Jelena
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5376
AB  - The mobility of chemical elements in the soil-orchid system has been poorly studied. The aim of this study is to evaluate the uptake and mobility of several trace (Li, Ba, Sr, Ag, Hg, and B) and macronutrients (Ca, Mg, and K) in the orchid Anacamptis morio (L.) R.M.Bateman, Pridgeon & M.W.Chase from soils in western Serbia. The sampling sites are characterized by three different bedrock types—cherts, limestones, and serpentines, which are the source of the significant chemical differences in the elemental status of the soil and plant tissues. The four-step Community Bureau of Reference sequential extraction procedure was used to determine the distribution of fractions and predict their potential phytoavailability. The orchid and soil samples were analyzed for total elemental content analysis using ICP-OES. The greatest potential for plant availability was determined for Ba and Sr, representing about 80% of the total soil content. More than 40% of Li in the soils was found to be potentially phytoavailable. Significant correlations were found between the total content of Li, B, and Sr in soils. Between 38 and 60% of Li content and more than 80% of Ba and Sr content were determined to be potentially phytoavailable by sequential analysis. The highest bioconcentration factor (> 1) was determined in the case of B and Sr for all orchid organs, while translocation factor for Li was highest in tubers and leaves. The studied elements were mainly stored in tubers and roots, indicating the exclusion strategy of A. morio as a metal tolerance mechanism. The data obtained showed significant differences in metal content in soils and plants originating from sites with different parent materials, suggesting that bedrock type and associated soil properties are important factors that determine chemical element mobility and uptake.
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types
DO  - 10.1007/s11356-022-22110-z
ER  - 

@article{
author = "Mikavica, Ivana and Ranđelović, Dragana and Đorđević, Vladan and Rakić, Tamara and Gajić, Gordana and Mutić, Jelena",
year = "2022",
abstract = "The mobility of chemical elements in the soil-orchid system has been poorly studied. The aim of this study is to evaluate the uptake and mobility of several trace (Li, Ba, Sr, Ag, Hg, and B) and macronutrients (Ca, Mg, and K) in the orchid Anacamptis morio (L.) R.M.Bateman, Pridgeon & M.W.Chase from soils in western Serbia. The sampling sites are characterized by three different bedrock types—cherts, limestones, and serpentines, which are the source of the significant chemical differences in the elemental status of the soil and plant tissues. The four-step Community Bureau of Reference sequential extraction procedure was used to determine the distribution of fractions and predict their potential phytoavailability. The orchid and soil samples were analyzed for total elemental content analysis using ICP-OES. The greatest potential for plant availability was determined for Ba and Sr, representing about 80% of the total soil content. More than 40% of Li in the soils was found to be potentially phytoavailable. Significant correlations were found between the total content of Li, B, and Sr in soils. Between 38 and 60% of Li content and more than 80% of Ba and Sr content were determined to be potentially phytoavailable by sequential analysis. The highest bioconcentration factor (> 1) was determined in the case of B and Sr for all orchid organs, while translocation factor for Li was highest in tubers and leaves. The studied elements were mainly stored in tubers and roots, indicating the exclusion strategy of A. morio as a metal tolerance mechanism. The data obtained showed significant differences in metal content in soils and plants originating from sites with different parent materials, suggesting that bedrock type and associated soil properties are important factors that determine chemical element mobility and uptake.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types",
doi = "10.1007/s11356-022-22110-z"
}

Mikavica, I., Ranđelović, D., Đorđević, V., Rakić, T., Gajić, G.,& Mutić, J.. (2022). Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types. in Environmental Science and Pollution Research
Springer..
https://doi.org/10.1007/s11356-022-22110-z

Mikavica I, Ranđelović D, Đorđević V, Rakić T, Gajić G, Mutić J. Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types. in Environmental Science and Pollution Research. 2022;.
doi:10.1007/s11356-022-22110-z .

Mikavica, Ivana, Ranđelović, Dragana, Đorđević, Vladan, Rakić, Tamara, Gajić, Gordana, Mutić, Jelena, "Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types" in Environmental Science and Pollution Research (2022),
https://doi.org/10.1007/s11356-022-22110-z . .

TY  - JOUR
AU  - Gođevac, Dejan
AU  - Ivanović, Stefan
AU  - Simić, Katarina
AU  - Anđelković, Boban
AU  - Jovanović, Živko
AU  - Rakić, Tamara
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5165
AB  - Introduction
Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated.

Objective
To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process

Methods
Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates.

Results
Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples.

Conclusion
Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.
PB  - Willey
T2  - Phytochemical Analysis
T1  - Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae
VL  - 33
IS  - 6
SP  - 961
SP  - 961
EP  - 970
DO  - 10.1002/pca.3151
ER  - 

@article{
author = "Gođevac, Dejan and Ivanović, Stefan and Simić, Katarina and Anđelković, Boban and Jovanović, Živko and Rakić, Tamara",
year = "2022",
abstract = "Introduction
Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated.

Objective
To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process

Methods
Proton nuclear magnetic resonance (1H-NMR) and gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates.

Results
Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples.

Conclusion
Using 1H-NMR and GC–MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.",
publisher = "Willey",
journal = "Phytochemical Analysis",
title = "Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae",
volume = "33",
number = "6",
pages = "961-961-970",
doi = "10.1002/pca.3151"
}

Gođevac, D., Ivanović, S., Simić, K., Anđelković, B., Jovanović, Ž.,& Rakić, T.. (2022). Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae. in Phytochemical Analysis
Willey., 33(6), 961-970.
https://doi.org/10.1002/pca.3151

Gođevac D, Ivanović S, Simić K, Anđelković B, Jovanović Ž, Rakić T. Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae. in Phytochemical Analysis. 2022;33(6):961-970.
doi:10.1002/pca.3151 .

Gođevac, Dejan, Ivanović, Stefan, Simić, Katarina, Anđelković, Boban, Jovanović, Živko, Rakić, Tamara, "Metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and R. nathaliae" in Phytochemical Analysis, 33, no. 6 (2022):961-970,
https://doi.org/10.1002/pca.3151 . .

TY  - CONF
AU  - Ivanović, Stefan
AU  - Gođevac, Dejan
AU  - Simić, Katarina
AU  - Anđelković, Boban
AU  - Jovanović, Ž. D.
AU  - Rakić, Tamara
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5522
AB  - Ramonda serbica and R. nathaliae are resurrection plants that have the
remarkable ability to survive the complete desiccation during periods of drought and
rapidly revive when rewatered and rehydrated. To investigate metabolic changes
during their desiccation and recovery process NMR-based metabolomics approach
coupled with multivariate data analysis was utilized to identify the metabolomes of
the plants from 90 biological replicates. The NMR metabolomics profiles of R.
serbica and R. nathaliae were subjected to multivariate data analysis. PCA was
performed, which resulted in eight principal components (PCs) in both models,
explaining 77.0% of the total data variance in the model with R. serbica samples,
and 79.5% of the variance in the model with R. nathaliae samples. Using NMR
experiments, the content of the two most dominant polar components found in the
leaves of these two plants was determined. Sucrose and the polyphenolic glycoside
myconoside were predominant in almost equal amounts in all samples studied,
regardless of their water content at sampling. Using of 1D and 2D NMR
experiments the main components have been successfully identified. Also, it was
necessary to isolate and purify the myconoside to confirm the structure.
PB  - Niš : Department of Biology and Ecology, Faculty of Science and  Mathematics
PB  - Belgrade : Institute for Nature Conservation of  Serbia
C3  - Book of Abstracts - 14th Symposium on the Flora of Southeastern  Serbia and Neighboring Regions, 26th to 29th June, 2022, Kladovo
T1  - NMR metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and Ramonda nathaliae
SP  - 171
EP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_cer_5522
ER  - 

@conference{
author = "Ivanović, Stefan and Gođevac, Dejan and Simić, Katarina and Anđelković, Boban and Jovanović, Ž. D. and Rakić, Tamara",
year = "2022",
abstract = "Ramonda serbica and R. nathaliae are resurrection plants that have the
remarkable ability to survive the complete desiccation during periods of drought and
rapidly revive when rewatered and rehydrated. To investigate metabolic changes
during their desiccation and recovery process NMR-based metabolomics approach
coupled with multivariate data analysis was utilized to identify the metabolomes of
the plants from 90 biological replicates. The NMR metabolomics profiles of R.
serbica and R. nathaliae were subjected to multivariate data analysis. PCA was
performed, which resulted in eight principal components (PCs) in both models,
explaining 77.0% of the total data variance in the model with R. serbica samples,
and 79.5% of the variance in the model with R. nathaliae samples. Using NMR
experiments, the content of the two most dominant polar components found in the
leaves of these two plants was determined. Sucrose and the polyphenolic glycoside
myconoside were predominant in almost equal amounts in all samples studied,
regardless of their water content at sampling. Using of 1D and 2D NMR
experiments the main components have been successfully identified. Also, it was
necessary to isolate and purify the myconoside to confirm the structure.",
publisher = "Niš : Department of Biology and Ecology, Faculty of Science and  Mathematics, Belgrade : Institute for Nature Conservation of  Serbia",
journal = "Book of Abstracts - 14th Symposium on the Flora of Southeastern  Serbia and Neighboring Regions, 26th to 29th June, 2022, Kladovo",
title = "NMR metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and Ramonda nathaliae",
pages = "171-171",
url = "https://hdl.handle.net/21.15107/rcub_cer_5522"
}

Ivanović, S., Gođevac, D., Simić, K., Anđelković, B., Jovanović, Ž. D.,& Rakić, T.. (2022). NMR metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and Ramonda nathaliae. in Book of Abstracts - 14th Symposium on the Flora of Southeastern  Serbia and Neighboring Regions, 26th to 29th June, 2022, Kladovo
Niš : Department of Biology and Ecology, Faculty of Science and  Mathematics., 171-171.
https://hdl.handle.net/21.15107/rcub_cer_5522

Ivanović S, Gođevac D, Simić K, Anđelković B, Jovanović ŽD, Rakić T. NMR metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and Ramonda nathaliae. in Book of Abstracts - 14th Symposium on the Flora of Southeastern  Serbia and Neighboring Regions, 26th to 29th June, 2022, Kladovo. 2022;:171-171.
https://hdl.handle.net/21.15107/rcub_cer_5522 .

Ivanović, Stefan, Gođevac, Dejan, Simić, Katarina, Anđelković, Boban, Jovanović, Ž. D., Rakić, Tamara, "NMR metabolomics study of the desiccation and recovery process in the resurrection plants Ramonda serbica and Ramonda nathaliae" in Book of Abstracts - 14th Symposium on the Flora of Southeastern  Serbia and Neighboring Regions, 26th to 29th June, 2022, Kladovo (2022):171-171,
https://hdl.handle.net/21.15107/rcub_cer_5522 .

TY  - CONF
AU  - Gođevac, Dejan
AU  - Simić, Katarina
AU  - Ivanović, Stefan
AU  - Anđelković, Boban
AU  - Jovanović, Živko
AU  - Rakić, Tamara
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5524
AB  - Ramonda serbica represents the remnant of the Tertiary tropical and subtropical flora in Europe and is the rare resurrection plants of Northern Hemisphere temperate zone. The plant remains well-hydrated during spring, late autumn and in winter. In summer and early autumn when plants are subjected to drought, their desiccation tolerance comes into operation and they spend it in anabiosis [1]. In this study, metabolic responses to dehydration and rehydration of R. serbica, were investigated. For this purpose, GC-MS/FID
based metabolomics method was performed. Leaves from the control (well-watered), dehydrated and partially rehydrated plants were sampled. Each leaf was powdered using liquid nitrogen and then freeze-dried. The internal standard (10-undecenoic acid) was added to the dry plant material and then extracted in methanol/water using ultra-sonication. After centrifugation of the mixture, the supernatant was dried and derivatized using two-step procedure involving oximation and silylation. GC-MS/FID analysis of each sample was then performed. The metabolites were identified using El-MS spectra. The areas from FID chromatograms were used for multivariate data analysis. Two OPLS-DA models were applied to investigate dehydration and rehydration process. According to high VIP scores, sugars, such as fructose, glucose, galactinol, sucrose, together with glyceric acid, xylonic acid 8-lactone and aspartic acid were found to be the most influential in the OPLS-DA models.
PB  - ICNPU-2019
C3  - Book of abstracts - 4th international conference on  natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria
T1  - GC-MS based metabolomics study of the resurrection plant Ramonda serbica
SP  - 189
EP  - 190
UR  - https://hdl.handle.net/21.15107/rcub_cer_5524
ER  - 

@conference{
author = "Gođevac, Dejan and Simić, Katarina and Ivanović, Stefan and Anđelković, Boban and Jovanović, Živko and Rakić, Tamara",
year = "2019",
abstract = "Ramonda serbica represents the remnant of the Tertiary tropical and subtropical flora in Europe and is the rare resurrection plants of Northern Hemisphere temperate zone. The plant remains well-hydrated during spring, late autumn and in winter. In summer and early autumn when plants are subjected to drought, their desiccation tolerance comes into operation and they spend it in anabiosis [1]. In this study, metabolic responses to dehydration and rehydration of R. serbica, were investigated. For this purpose, GC-MS/FID
based metabolomics method was performed. Leaves from the control (well-watered), dehydrated and partially rehydrated plants were sampled. Each leaf was powdered using liquid nitrogen and then freeze-dried. The internal standard (10-undecenoic acid) was added to the dry plant material and then extracted in methanol/water using ultra-sonication. After centrifugation of the mixture, the supernatant was dried and derivatized using two-step procedure involving oximation and silylation. GC-MS/FID analysis of each sample was then performed. The metabolites were identified using El-MS spectra. The areas from FID chromatograms were used for multivariate data analysis. Two OPLS-DA models were applied to investigate dehydration and rehydration process. According to high VIP scores, sugars, such as fructose, glucose, galactinol, sucrose, together with glyceric acid, xylonic acid 8-lactone and aspartic acid were found to be the most influential in the OPLS-DA models.",
publisher = "ICNPU-2019",
journal = "Book of abstracts - 4th international conference on  natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria",
title = "GC-MS based metabolomics study of the resurrection plant Ramonda serbica",
pages = "189-190",
url = "https://hdl.handle.net/21.15107/rcub_cer_5524"
}

Gođevac, D., Simić, K., Ivanović, S., Anđelković, B., Jovanović, Ž.,& Rakić, T.. (2019). GC-MS based metabolomics study of the resurrection plant Ramonda serbica. in Book of abstracts - 4th international conference on  natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria
ICNPU-2019., 189-190.
https://hdl.handle.net/21.15107/rcub_cer_5524

Gođevac D, Simić K, Ivanović S, Anđelković B, Jovanović Ž, Rakić T. GC-MS based metabolomics study of the resurrection plant Ramonda serbica. in Book of abstracts - 4th international conference on  natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria. 2019;:189-190.
https://hdl.handle.net/21.15107/rcub_cer_5524 .

Gođevac, Dejan, Simić, Katarina, Ivanović, Stefan, Anđelković, Boban, Jovanović, Živko, Rakić, Tamara, "GC-MS based metabolomics study of the resurrection plant Ramonda serbica" in Book of abstracts - 4th international conference on  natural products utilization: from plants to pharmacy shelf, 29/05-01/06 2019, Albena, Bulgaria (2019):189-190,
https://hdl.handle.net/21.15107/rcub_cer_5524 .