Moric, Ivana

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ab43dc86-cdf8-4fba-8cb2-3cb14f058673
  • Moric, Ivana (6)
Projects

Author's Bibliography

Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo

Djokic, Lidija; Stankovic, Nada; Galic, Ivana; Moric, Ivana; Radakovic, Natasa; Šegan, Sandra; Pavic, Aleksandar; Senerovic, Lidija

(Frontiers Media SA, 2022)

TY  - JOUR
AU  - Djokic, Lidija
AU  - Stankovic, Nada
AU  - Galic, Ivana
AU  - Moric, Ivana
AU  - Radakovic, Natasa
AU  - Šegan, Sandra
AU  - Pavic, Aleksandar
AU  - Senerovic, Lidija
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5579
AB  - Bacterial infections have become increasingly difficult to treat due to the occurrence of antibiotic-resistant strains. A promising strategy to increase the efficacy of therapy is to combine antibacterials with agents that decrease pathogen virulence via the modulation of the quorum sensing (QS). Lactonases inhibit acylated homoserine lactone (AHL)-mediated QS in Gram-negative bacteria, including the leading nosocomial pathogen Pseudomonas aeruginosa. Here we describe the characteristics of heterologously expressed YtnP lactonase from Bacillus paralicheniformis ZP1 (YtnP-ZP1) isolated from agricultural soil using the culture enrichment method. Purified YtnP-ZP1 hydrolyzed different AHLs with preference to substrates with long acyl residues as evaluated in assays with biosensors and HPLC. The enzyme showed good thermostability and activity in a wide temperature range. YtnP-ZP1 in 50 μg mL–1 concentration reduced the amount of P. aeruginosa-produced long-chain AHLs by 85%, while it hydrolyzed 50% of short-chain AHLs. Incubation of P. aeruginosa PAO1 with YtnP-ZP1 reduced its swarming motility and elastolytic activity without bactericidal effect. YtnP-ZP1 caused the inhibition of biofilm formation and disintegration of mature biofilms in P. aeruginosa PAO1 and multiresistant clinical strain BR5H that was visualized by crystal violet staining. The treatment with YtnP-ZP1 in concentrations higher than 25 μg mL–1 improved the survival of P. aeruginosa PAO1-infected zebrafish (Danio rerio), rescuing 80% of embryos, while in combination with tobramycin or gentamicin survival rate increased to 100%. The treatment of P. aeruginosa PAO1 biofilms on infected zebrafish tail wounds with 50 μg mL–1 YtnP-ZP1 and 2 × MIC tobramycin led to infection clearing in 2 days. The extensive toxicity studies proved YtnP-ZP1 was non-toxic to human cells and zebrafish. In conclusion, novel YtnP-ZP1 lactonase with its effective anti-virulence activity could be used to increase the efficacy of clinically approved antibiotics in clearing both systemic and biofilm-associated P. aeruginosa infections.
PB  - Frontiers Media SA
T2  - Frontiers in Microbiology
T1  - Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo
VL  - 13
SP  - 906312
DO  - 10.3389/fmicb.2022.906312
ER  - 
@article{
author = "Djokic, Lidija and Stankovic, Nada and Galic, Ivana and Moric, Ivana and Radakovic, Natasa and Šegan, Sandra and Pavic, Aleksandar and Senerovic, Lidija",
year = "2022",
abstract = "Bacterial infections have become increasingly difficult to treat due to the occurrence of antibiotic-resistant strains. A promising strategy to increase the efficacy of therapy is to combine antibacterials with agents that decrease pathogen virulence via the modulation of the quorum sensing (QS). Lactonases inhibit acylated homoserine lactone (AHL)-mediated QS in Gram-negative bacteria, including the leading nosocomial pathogen Pseudomonas aeruginosa. Here we describe the characteristics of heterologously expressed YtnP lactonase from Bacillus paralicheniformis ZP1 (YtnP-ZP1) isolated from agricultural soil using the culture enrichment method. Purified YtnP-ZP1 hydrolyzed different AHLs with preference to substrates with long acyl residues as evaluated in assays with biosensors and HPLC. The enzyme showed good thermostability and activity in a wide temperature range. YtnP-ZP1 in 50 μg mL–1 concentration reduced the amount of P. aeruginosa-produced long-chain AHLs by 85%, while it hydrolyzed 50% of short-chain AHLs. Incubation of P. aeruginosa PAO1 with YtnP-ZP1 reduced its swarming motility and elastolytic activity without bactericidal effect. YtnP-ZP1 caused the inhibition of biofilm formation and disintegration of mature biofilms in P. aeruginosa PAO1 and multiresistant clinical strain BR5H that was visualized by crystal violet staining. The treatment with YtnP-ZP1 in concentrations higher than 25 μg mL–1 improved the survival of P. aeruginosa PAO1-infected zebrafish (Danio rerio), rescuing 80% of embryos, while in combination with tobramycin or gentamicin survival rate increased to 100%. The treatment of P. aeruginosa PAO1 biofilms on infected zebrafish tail wounds with 50 μg mL–1 YtnP-ZP1 and 2 × MIC tobramycin led to infection clearing in 2 days. The extensive toxicity studies proved YtnP-ZP1 was non-toxic to human cells and zebrafish. In conclusion, novel YtnP-ZP1 lactonase with its effective anti-virulence activity could be used to increase the efficacy of clinically approved antibiotics in clearing both systemic and biofilm-associated P. aeruginosa infections.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Microbiology",
title = "Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo",
volume = "13",
pages = "906312",
doi = "10.3389/fmicb.2022.906312"
}
Djokic, L., Stankovic, N., Galic, I., Moric, I., Radakovic, N., Šegan, S., Pavic, A.,& Senerovic, L.. (2022). Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo. in Frontiers in Microbiology
Frontiers Media SA., 13, 906312.
https://doi.org/10.3389/fmicb.2022.906312
Djokic L, Stankovic N, Galic I, Moric I, Radakovic N, Šegan S, Pavic A, Senerovic L. Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo. in Frontiers in Microbiology. 2022;13:906312.
doi:10.3389/fmicb.2022.906312 .
Djokic, Lidija, Stankovic, Nada, Galic, Ivana, Moric, Ivana, Radakovic, Natasa, Šegan, Sandra, Pavic, Aleksandar, Senerovic, Lidija, "Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo" in Frontiers in Microbiology, 13 (2022):906312,
https://doi.org/10.3389/fmicb.2022.906312 . .
3
12
6

Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections

Senerovic, Lidija; Moric, Ivana; Milivojevic, Dusan; Opsenica, Dejan

(Elsevier, 2020)

TY  - CHAP
AU  - Senerovic, Lidija
AU  - Moric, Ivana
AU  - Milivojevic, Dusan
AU  - Opsenica, Dejan
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4459
AB  - The rapid emergence of antibiotic-resistant pathogens presents a global healthcare challenge. Bacteria control their virulence, motility, and biofilm formation, all of them being required for establishing pathogenicity, through a cell density-dependent communication system known as quorum sensing (QS). QS comprises production of extracellular signaling molecules, their detection, and population-wide response involving regulation of the virulence genes expression. Inhibition of QS affects virulence and reduces harmful effects to the host and as such presents a promising strategy to fight antibiotic-resistant infections. Multiresistant Pseudomonas aeruginosa belogns to the group of most critical pathogens for which the introduction of new therapeutics is imperative. In the search for novel therapeutics nature continues to be inexhaustible source of bioactive scaffolds, which provide the bases for structure-based rational drug design enabling further exploitation of diverse natural structures. This review describes bacterial QS systems, highlights strategies for their manipulation, overviews nature-inspired antivirulence molecules primarily against P. aeruginosa, and discusses their perspectives.
PB  - Elsevier
T2  - Biodiversity and Biomedicine
T1  - Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections
SP  - 497
EP  - 523
DO  - 10.1016/B978-0-12-819541-3.00025-6
ER  - 
@inbook{
author = "Senerovic, Lidija and Moric, Ivana and Milivojevic, Dusan and Opsenica, Dejan",
year = "2020",
abstract = "The rapid emergence of antibiotic-resistant pathogens presents a global healthcare challenge. Bacteria control their virulence, motility, and biofilm formation, all of them being required for establishing pathogenicity, through a cell density-dependent communication system known as quorum sensing (QS). QS comprises production of extracellular signaling molecules, their detection, and population-wide response involving regulation of the virulence genes expression. Inhibition of QS affects virulence and reduces harmful effects to the host and as such presents a promising strategy to fight antibiotic-resistant infections. Multiresistant Pseudomonas aeruginosa belogns to the group of most critical pathogens for which the introduction of new therapeutics is imperative. In the search for novel therapeutics nature continues to be inexhaustible source of bioactive scaffolds, which provide the bases for structure-based rational drug design enabling further exploitation of diverse natural structures. This review describes bacterial QS systems, highlights strategies for their manipulation, overviews nature-inspired antivirulence molecules primarily against P. aeruginosa, and discusses their perspectives.",
publisher = "Elsevier",
journal = "Biodiversity and Biomedicine",
booktitle = "Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections",
pages = "497-523",
doi = "10.1016/B978-0-12-819541-3.00025-6"
}
Senerovic, L., Moric, I., Milivojevic, D.,& Opsenica, D.. (2020). Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections. in Biodiversity and Biomedicine
Elsevier., 497-523.
https://doi.org/10.1016/B978-0-12-819541-3.00025-6
Senerovic L, Moric I, Milivojevic D, Opsenica D. Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections. in Biodiversity and Biomedicine. 2020;:497-523.
doi:10.1016/B978-0-12-819541-3.00025-6 .
Senerovic, Lidija, Moric, Ivana, Milivojevic, Dusan, Opsenica, Dejan, "Nature-inspired synthetic analogues of quorum sensing signaling molecules as novel therapeutics against Pseudomonas aeruginosa infections" in Biodiversity and Biomedicine (2020):497-523,
https://doi.org/10.1016/B978-0-12-819541-3.00025-6 . .
1

Quinolines and Quinolones as Antibacterial, Antifungal, Antivirulence, Antiviral and Anti-parasitic Agents

Senerovic, Lidija; Opsenica, Dejan; Moric, Ivana; Aleksic, Ivana; Spasić, Marta; Vasiljevic, Branka

(Springer Nature, 2019)

TY  - CHAP
AU  - Senerovic, Lidija
AU  - Opsenica, Dejan
AU  - Moric, Ivana
AU  - Aleksic, Ivana
AU  - Spasić, Marta
AU  - Vasiljevic, Branka
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3094
AB  - Infective diseases have become health threat of
a global proportion due to appearance and
spread of microorganisms resistant to majority
of therapeutics currently used for their treatment.
Therefore, there is a constant need for
development of new antimicrobial agents, as
well as novel therapeutic strategies.
Quinolines and quinolones, isolated from
plants, animals, and microorganisms, have
demonstrated numerous biological activities
such as antimicrobial, insecticidal, antiinflammatory,
antiplatelet, and antitumor. For
more than two centuries quinoline/quinolone
moiety has been used as a scaffold for drug
development and even today it represents an
inexhaustible inspiration for design and development
of novel semi-synthetic or synthetic
agents exhibiting broad spectrum of
bioactivities. The structural diversity of
synthetized compounds provides high and
selective activity attained through different
mechanisms of action, as well as low toxicity
on human cells. This review describes quinoline
and quinolone derivatives with
antibacterial, antifungal, anti-virulent,
antiviral, and anti-parasitic activities with the
focus on the last 10 years literature.
PB  - Springer Nature
T2  - Advances in Experimental Medicine and Biology - Advances in Microbiology, Infectious Diseases and Public Health
T1  - Quinolines and Quinolones as Antibacterial, Antifungal, Antivirulence, Antiviral and Anti-parasitic Agents
DO  - 10.1007/5584_2019_428
ER  - 
@inbook{
author = "Senerovic, Lidija and Opsenica, Dejan and Moric, Ivana and Aleksic, Ivana and Spasić, Marta and Vasiljevic, Branka",
year = "2019",
abstract = "Infective diseases have become health threat of
a global proportion due to appearance and
spread of microorganisms resistant to majority
of therapeutics currently used for their treatment.
Therefore, there is a constant need for
development of new antimicrobial agents, as
well as novel therapeutic strategies.
Quinolines and quinolones, isolated from
plants, animals, and microorganisms, have
demonstrated numerous biological activities
such as antimicrobial, insecticidal, antiinflammatory,
antiplatelet, and antitumor. For
more than two centuries quinoline/quinolone
moiety has been used as a scaffold for drug
development and even today it represents an
inexhaustible inspiration for design and development
of novel semi-synthetic or synthetic
agents exhibiting broad spectrum of
bioactivities. The structural diversity of
synthetized compounds provides high and
selective activity attained through different
mechanisms of action, as well as low toxicity
on human cells. This review describes quinoline
and quinolone derivatives with
antibacterial, antifungal, anti-virulent,
antiviral, and anti-parasitic activities with the
focus on the last 10 years literature.",
publisher = "Springer Nature",
journal = "Advances in Experimental Medicine and Biology - Advances in Microbiology, Infectious Diseases and Public Health",
booktitle = "Quinolines and Quinolones as Antibacterial, Antifungal, Antivirulence, Antiviral and Anti-parasitic Agents",
doi = "10.1007/5584_2019_428"
}
Senerovic, L., Opsenica, D., Moric, I., Aleksic, I., Spasić, M.,& Vasiljevic, B.. (2019). Quinolines and Quinolones as Antibacterial, Antifungal, Antivirulence, Antiviral and Anti-parasitic Agents. in Advances in Experimental Medicine and Biology - Advances in Microbiology, Infectious Diseases and Public Health
Springer Nature..
https://doi.org/10.1007/5584_2019_428
Senerovic L, Opsenica D, Moric I, Aleksic I, Spasić M, Vasiljevic B. Quinolines and Quinolones as Antibacterial, Antifungal, Antivirulence, Antiviral and Anti-parasitic Agents. in Advances in Experimental Medicine and Biology - Advances in Microbiology, Infectious Diseases and Public Health. 2019;.
doi:10.1007/5584_2019_428 .
Senerovic, Lidija, Opsenica, Dejan, Moric, Ivana, Aleksic, Ivana, Spasić, Marta, Vasiljevic, Branka, "Quinolines and Quinolones as Antibacterial, Antifungal, Antivirulence, Antiviral and Anti-parasitic Agents" in Advances in Experimental Medicine and Biology - Advances in Microbiology, Infectious Diseases and Public Health (2019),
https://doi.org/10.1007/5584_2019_428 . .
68
59

Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa

Aleksić, Ivana; Šegan, Sandra; Andrić, Filip; Zlatović, Mario; Moric, Ivana; Opsenica, Dejan; Senerovic, Lidija

(American Chemical Society (ACS), 2017)

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Šegan, Sandra
AU  - Andrić, Filip
AU  - Zlatović, Mario
AU  - Moric, Ivana
AU  - Opsenica, Dejan
AU  - Senerovic, Lidija
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2270
AB  - Antibiotic resistance has become a serious global threat to public health; therefore, improved strategies and structurally novel antimicrobials are urgently needed to combat infectious diseases. Here we report a new type of highly potent 4-aminoquinoline derivatives as quorum sensing inhibitors in Serratia marcescens and Pseudomonas aeruginosa, exhibiting weak bactericidal activities (minimum inhibitory concentration (MIC) > 400 mu M). Through detailed structure-activity study, we have identified 7-Cl and 7-CF3 substituted N-dodecylamino-4-aminoquinolines (5 and 10) as biofilm formation inhibitors with 50% biofilm inhibition at 69 mu M and 63 mu M in S. marcescens and P. aeruginosa, respectively. These two compounds, 5 and 10, are the first quinoline derivatives with anti-biofilm formation activity reported in S. marcescens. Quantitative structure-activity relationship (QSAR) analysis identified structural descriptors such as Wiener indices, hyper-distance-path index (HDPI), mean topological charge (MTC), topological charge index (TCI), and log D(o/w)exp as the most influential in biofilm inhibition in this bacterial species. Derivative 10 is one of the most potent quinoline type inhibitors of pyocyanin production described so far (IC50 = 2.5 mu M). While we have demonstrated that 5 and 10 act as Pseudomonas quinolone system (PQS) antagonists, the mechanism of inhibition of S. marcescens biofilm formation with these compounds remains open since signaling similar to P. aeruginosa PQS system has not yet been described in Serratia and activity of these compounds on acylhomoserine lactone (AHL) signaling has not been detected. Our data show that 7-Cl and 7-CF3 substituted N-dodecylamino-4-aminoquinolines present the promising scaffolds for developing antivirulence and anti-biofilm formation agents against multidrug-resistant bacterial species.
PB  - American Chemical Society (ACS)
T2  - Acs Chemical Biology
T1  - Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa
VL  - 12
IS  - 5
SP  - 1425
EP  - 1434
DO  - 10.1021/acschembio.6b01149
ER  - 
@article{
author = "Aleksić, Ivana and Šegan, Sandra and Andrić, Filip and Zlatović, Mario and Moric, Ivana and Opsenica, Dejan and Senerovic, Lidija",
year = "2017",
abstract = "Antibiotic resistance has become a serious global threat to public health; therefore, improved strategies and structurally novel antimicrobials are urgently needed to combat infectious diseases. Here we report a new type of highly potent 4-aminoquinoline derivatives as quorum sensing inhibitors in Serratia marcescens and Pseudomonas aeruginosa, exhibiting weak bactericidal activities (minimum inhibitory concentration (MIC) > 400 mu M). Through detailed structure-activity study, we have identified 7-Cl and 7-CF3 substituted N-dodecylamino-4-aminoquinolines (5 and 10) as biofilm formation inhibitors with 50% biofilm inhibition at 69 mu M and 63 mu M in S. marcescens and P. aeruginosa, respectively. These two compounds, 5 and 10, are the first quinoline derivatives with anti-biofilm formation activity reported in S. marcescens. Quantitative structure-activity relationship (QSAR) analysis identified structural descriptors such as Wiener indices, hyper-distance-path index (HDPI), mean topological charge (MTC), topological charge index (TCI), and log D(o/w)exp as the most influential in biofilm inhibition in this bacterial species. Derivative 10 is one of the most potent quinoline type inhibitors of pyocyanin production described so far (IC50 = 2.5 mu M). While we have demonstrated that 5 and 10 act as Pseudomonas quinolone system (PQS) antagonists, the mechanism of inhibition of S. marcescens biofilm formation with these compounds remains open since signaling similar to P. aeruginosa PQS system has not yet been described in Serratia and activity of these compounds on acylhomoserine lactone (AHL) signaling has not been detected. Our data show that 7-Cl and 7-CF3 substituted N-dodecylamino-4-aminoquinolines present the promising scaffolds for developing antivirulence and anti-biofilm formation agents against multidrug-resistant bacterial species.",
publisher = "American Chemical Society (ACS)",
journal = "Acs Chemical Biology",
title = "Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa",
volume = "12",
number = "5",
pages = "1425-1434",
doi = "10.1021/acschembio.6b01149"
}
Aleksić, I., Šegan, S., Andrić, F., Zlatović, M., Moric, I., Opsenica, D.,& Senerovic, L.. (2017). Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa. in Acs Chemical Biology
American Chemical Society (ACS)., 12(5), 1425-1434.
https://doi.org/10.1021/acschembio.6b01149
Aleksić I, Šegan S, Andrić F, Zlatović M, Moric I, Opsenica D, Senerovic L. Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa. in Acs Chemical Biology. 2017;12(5):1425-1434.
doi:10.1021/acschembio.6b01149 .
Aleksić, Ivana, Šegan, Sandra, Andrić, Filip, Zlatović, Mario, Moric, Ivana, Opsenica, Dejan, Senerovic, Lidija, "Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa" in Acs Chemical Biology, 12, no. 5 (2017):1425-1434,
https://doi.org/10.1021/acschembio.6b01149 . .
9
45
27
45

Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa

Aleksić, Ivana; Šegan, Sandra; Andrić, Filip; Zlatović, Mario; Moric, Ivana; Opsenica, Dejan; Senerovic, Lidija

(American Chemical Society (ACS), 2017)

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Šegan, Sandra
AU  - Andrić, Filip
AU  - Zlatović, Mario
AU  - Moric, Ivana
AU  - Opsenica, Dejan
AU  - Senerovic, Lidija
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2983
AB  - Antibiotic resistance has become a serious global threat to public health; therefore, improved strategies and structurally novel antimicrobials are urgently needed to combat infectious diseases. Here we report a new type of highly potent 4-aminoquinoline derivatives as quorum sensing inhibitors in Serratia marcescens and Pseudomonas aeruginosa, exhibiting weak bactericidal activities (minimum inhibitory concentration (MIC) > 400 mu M). Through detailed structure-activity study, we have identified 7-Cl and 7-CF3 substituted N-dodecylamino-4-aminoquinolines (5 and 10) as biofilm formation inhibitors with 50% biofilm inhibition at 69 mu M and 63 mu M in S. marcescens and P. aeruginosa, respectively. These two compounds, 5 and 10, are the first quinoline derivatives with anti-biofilm formation activity reported in S. marcescens. Quantitative structure-activity relationship (QSAR) analysis identified structural descriptors such as Wiener indices, hyper-distance-path index (HDPI), mean topological charge (MTC), topological charge index (TCI), and log D(o/w)exp as the most influential in biofilm inhibition in this bacterial species. Derivative 10 is one of the most potent quinoline type inhibitors of pyocyanin production described so far (IC50 = 2.5 mu M). While we have demonstrated that 5 and 10 act as Pseudomonas quinolone system (PQS) antagonists, the mechanism of inhibition of S. marcescens biofilm formation with these compounds remains open since signaling similar to P. aeruginosa PQS system has not yet been described in Serratia and activity of these compounds on acylhomoserine lactone (AHL) signaling has not been detected. Our data show that 7-Cl and 7-CF3 substituted N-dodecylamino-4-aminoquinolines present the promising scaffolds for developing antivirulence and anti-biofilm formation agents against multidrug-resistant bacterial species.
PB  - American Chemical Society (ACS)
T2  - Acs Chemical Biology
T1  - Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa
VL  - 12
IS  - 5
SP  - 1425
EP  - 1434
DO  - 10.1021/acschembio.6b01149
ER  - 
@article{
author = "Aleksić, Ivana and Šegan, Sandra and Andrić, Filip and Zlatović, Mario and Moric, Ivana and Opsenica, Dejan and Senerovic, Lidija",
year = "2017",
abstract = "Antibiotic resistance has become a serious global threat to public health; therefore, improved strategies and structurally novel antimicrobials are urgently needed to combat infectious diseases. Here we report a new type of highly potent 4-aminoquinoline derivatives as quorum sensing inhibitors in Serratia marcescens and Pseudomonas aeruginosa, exhibiting weak bactericidal activities (minimum inhibitory concentration (MIC) > 400 mu M). Through detailed structure-activity study, we have identified 7-Cl and 7-CF3 substituted N-dodecylamino-4-aminoquinolines (5 and 10) as biofilm formation inhibitors with 50% biofilm inhibition at 69 mu M and 63 mu M in S. marcescens and P. aeruginosa, respectively. These two compounds, 5 and 10, are the first quinoline derivatives with anti-biofilm formation activity reported in S. marcescens. Quantitative structure-activity relationship (QSAR) analysis identified structural descriptors such as Wiener indices, hyper-distance-path index (HDPI), mean topological charge (MTC), topological charge index (TCI), and log D(o/w)exp as the most influential in biofilm inhibition in this bacterial species. Derivative 10 is one of the most potent quinoline type inhibitors of pyocyanin production described so far (IC50 = 2.5 mu M). While we have demonstrated that 5 and 10 act as Pseudomonas quinolone system (PQS) antagonists, the mechanism of inhibition of S. marcescens biofilm formation with these compounds remains open since signaling similar to P. aeruginosa PQS system has not yet been described in Serratia and activity of these compounds on acylhomoserine lactone (AHL) signaling has not been detected. Our data show that 7-Cl and 7-CF3 substituted N-dodecylamino-4-aminoquinolines present the promising scaffolds for developing antivirulence and anti-biofilm formation agents against multidrug-resistant bacterial species.",
publisher = "American Chemical Society (ACS)",
journal = "Acs Chemical Biology",
title = "Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa",
volume = "12",
number = "5",
pages = "1425-1434",
doi = "10.1021/acschembio.6b01149"
}
Aleksić, I., Šegan, S., Andrić, F., Zlatović, M., Moric, I., Opsenica, D.,& Senerovic, L.. (2017). Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa. in Acs Chemical Biology
American Chemical Society (ACS)., 12(5), 1425-1434.
https://doi.org/10.1021/acschembio.6b01149
Aleksić I, Šegan S, Andrić F, Zlatović M, Moric I, Opsenica D, Senerovic L. Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa. in Acs Chemical Biology. 2017;12(5):1425-1434.
doi:10.1021/acschembio.6b01149 .
Aleksić, Ivana, Šegan, Sandra, Andrić, Filip, Zlatović, Mario, Moric, Ivana, Opsenica, Dejan, Senerovic, Lidija, "Long-Chain 4-Aminoquinolines as Quorum Sensing Inhibitors in Serratia marcescens and Pseudomonas aeruginosa" in Acs Chemical Biology, 12, no. 5 (2017):1425-1434,
https://doi.org/10.1021/acschembio.6b01149 . .
9
45
27

Microbial diversity and isolation of multiple metal-tolerant bacteria from surface and underground pits within the copper mining and smelting complex Bor

Bajkic, Sanja; Narancic, Tanja; Dokic, Lidija; Đorđević, Dragana; Nikodinović-Runić, Jasmina; Moric, Ivana; Vasiljevic, Branka

(Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd, 2013)

TY  - JOUR
AU  - Bajkic, Sanja
AU  - Narancic, Tanja
AU  - Dokic, Lidija
AU  - Đorđević, Dragana
AU  - Nikodinović-Runić, Jasmina
AU  - Moric, Ivana
AU  - Vasiljevic, Branka
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1306
AB  - The bacterial diversity of the surface and deep sediment of the Copper Mining and Smelting Complex Bor, Serbia, was investigated using culture-dependent and culture-independent approaches. Sequencing analysis of 16S rDNA libraries revealed greater bacterial diversity in the surface sediment of the mining complex (MS) in comparison to deeper mine sediment (MU). While in the MS sample members of seven different phylogenetic groups were detected, in the MU sample library representatives of only three different groups were detected. The use of a culture-dependent approach revealed the presence of only three bacterial groups in both samples: Actinobacteria, Firmicutes and Proteobacteria, while six isolates exhibiting the highest metal tolerance were members of Arthrobacter and Staphylococcus genera. The most promising isolate, MSI08, was able to grow in the presence of high concentrations of Cd2+ (535 μM), Ni2+(17 mM) and Cr6+ (38.5 mM) and as such this indigenous strain has potential in the bioremediation of the contaminated surrounds of the city of Bor.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of Biological Sciences
T1  - Microbial diversity and isolation of multiple metal-tolerant bacteria from surface and underground pits within the copper mining and smelting complex Bor
VL  - 65
IS  - 1
SP  - 375
EP  - 386
DO  - 10.2298/ABS1301375B
ER  - 
@article{
author = "Bajkic, Sanja and Narancic, Tanja and Dokic, Lidija and Đorđević, Dragana and Nikodinović-Runić, Jasmina and Moric, Ivana and Vasiljevic, Branka",
year = "2013",
abstract = "The bacterial diversity of the surface and deep sediment of the Copper Mining and Smelting Complex Bor, Serbia, was investigated using culture-dependent and culture-independent approaches. Sequencing analysis of 16S rDNA libraries revealed greater bacterial diversity in the surface sediment of the mining complex (MS) in comparison to deeper mine sediment (MU). While in the MS sample members of seven different phylogenetic groups were detected, in the MU sample library representatives of only three different groups were detected. The use of a culture-dependent approach revealed the presence of only three bacterial groups in both samples: Actinobacteria, Firmicutes and Proteobacteria, while six isolates exhibiting the highest metal tolerance were members of Arthrobacter and Staphylococcus genera. The most promising isolate, MSI08, was able to grow in the presence of high concentrations of Cd2+ (535 μM), Ni2+(17 mM) and Cr6+ (38.5 mM) and as such this indigenous strain has potential in the bioremediation of the contaminated surrounds of the city of Bor.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of Biological Sciences",
title = "Microbial diversity and isolation of multiple metal-tolerant bacteria from surface and underground pits within the copper mining and smelting complex Bor",
volume = "65",
number = "1",
pages = "375-386",
doi = "10.2298/ABS1301375B"
}
Bajkic, S., Narancic, T., Dokic, L., Đorđević, D., Nikodinović-Runić, J., Moric, I.,& Vasiljevic, B.. (2013). Microbial diversity and isolation of multiple metal-tolerant bacteria from surface and underground pits within the copper mining and smelting complex Bor. in Archives of Biological Sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 65(1), 375-386.
https://doi.org/10.2298/ABS1301375B
Bajkic S, Narancic T, Dokic L, Đorđević D, Nikodinović-Runić J, Moric I, Vasiljevic B. Microbial diversity and isolation of multiple metal-tolerant bacteria from surface and underground pits within the copper mining and smelting complex Bor. in Archives of Biological Sciences. 2013;65(1):375-386.
doi:10.2298/ABS1301375B .
Bajkic, Sanja, Narancic, Tanja, Dokic, Lidija, Đorđević, Dragana, Nikodinović-Runić, Jasmina, Moric, Ivana, Vasiljevic, Branka, "Microbial diversity and isolation of multiple metal-tolerant bacteria from surface and underground pits within the copper mining and smelting complex Bor" in Archives of Biological Sciences, 65, no. 1 (2013):375-386,
https://doi.org/10.2298/ABS1301375B . .
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