TY  - JOUR
AU  - Rašković, Božidar S.
AU  - Ćirić, Miloš
AU  - Koko, Vesna
AU  - Stanković, Marko B.
AU  - Živić, Ivana M.
AU  - Marković, Zoran Z.
AU  - Poleksić, Vesna D.
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4429
AB  - In order to determine effects of different types of supplementary feed on the histology of liver and intestine of carp reared in a semi-intensive system, a six-month field experiment was carried out. Carp yearlings were fed different supplemental feeds: cereals (CF), pelleted (PF) and extruded (EF) compound feeds. Hepatocytes histomorphometry revealed larger nuclear profile area of EF-fed fish compared to other two groups, while cytoplasmic profile area of the hepatocytes decreased in following sequence: CF > EF > PF. Lower nucleus/cytoplasm ratio was found in CF-fed fish compared to EF-and PF-fed fish. Striking, season dependant finding, was that regardless of the type of supplementary feed the number of lipid droplets in hepatocytes was highest in July and August, lipid depletion occurred in September and October, while glycogen was present in hepatocytes throughout the study. No size difference between groups was observed for enterocytes height, absorptive surface height and number of goblet cells in tunica mucosa, but a strong relationship between length of intestinal folds and fish weight was found. Histological analysis revealed a difference among three types of supplemental feed used in this field study: extruded or steam-pelleted feed induced better nutritional status of common carp in comparison to cereals. At the same time, histomorphometry indicated some differences between EF-and PF-fed fish.
PB  - Springer Nature
T2  - Biologia
T1  - Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: Histological implications
VL  - 71
IS  - 2
SP  - 212
EP  - 219
DO  - 10.1515/biolog-2016-0017
ER  - 

@article{
author = "Rašković, Božidar S. and Ćirić, Miloš and Koko, Vesna and Stanković, Marko B. and Živić, Ivana M. and Marković, Zoran Z. and Poleksić, Vesna D.",
year = "2016",
abstract = "In order to determine effects of different types of supplementary feed on the histology of liver and intestine of carp reared in a semi-intensive system, a six-month field experiment was carried out. Carp yearlings were fed different supplemental feeds: cereals (CF), pelleted (PF) and extruded (EF) compound feeds. Hepatocytes histomorphometry revealed larger nuclear profile area of EF-fed fish compared to other two groups, while cytoplasmic profile area of the hepatocytes decreased in following sequence: CF > EF > PF. Lower nucleus/cytoplasm ratio was found in CF-fed fish compared to EF-and PF-fed fish. Striking, season dependant finding, was that regardless of the type of supplementary feed the number of lipid droplets in hepatocytes was highest in July and August, lipid depletion occurred in September and October, while glycogen was present in hepatocytes throughout the study. No size difference between groups was observed for enterocytes height, absorptive surface height and number of goblet cells in tunica mucosa, but a strong relationship between length of intestinal folds and fish weight was found. Histological analysis revealed a difference among three types of supplemental feed used in this field study: extruded or steam-pelleted feed induced better nutritional status of common carp in comparison to cereals. At the same time, histomorphometry indicated some differences between EF-and PF-fed fish.",
publisher = "Springer Nature",
journal = "Biologia",
title = "Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: Histological implications",
volume = "71",
number = "2",
pages = "212-219",
doi = "10.1515/biolog-2016-0017"
}

Rašković, B. S., Ćirić, M., Koko, V., Stanković, M. B., Živić, I. M., Marković, Z. Z.,& Poleksić, V. D.. (2016). Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: Histological implications. in Biologia
Springer Nature., 71(2), 212-219.
https://doi.org/10.1515/biolog-2016-0017

Rašković BS, Ćirić M, Koko V, Stanković MB, Živić IM, Marković ZZ, Poleksić VD. Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: Histological implications. in Biologia. 2016;71(2):212-219.
doi:10.1515/biolog-2016-0017 .

Rašković, Božidar S., Ćirić, Miloš, Koko, Vesna, Stanković, Marko B., Živić, Ivana M., Marković, Zoran Z., Poleksić, Vesna D., "Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: Histological implications" in Biologia, 71, no. 2 (2016):212-219,
https://doi.org/10.1515/biolog-2016-0017 . .

TY  - JOUR
AU  - Raskovic, Bozidar
AU  - Cicovacki, Stanislav
AU  - Ćirić, Miloš
AU  - Markovic, Zoran
AU  - Poleksić, Vesna
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1878
AB  - The semi-intensive system of common carp mono-culture in earthen ponds is currently the most practiced fish rearing system in several countries in Eastern Europe. This system is based on the addition of supplemented feed to ponds to achieve faster growth. In this study, the general state of the fish health was monitored when three different types of feeds were used: grain cereals (CF), commercial pelleted feed and extruded feed. The markers used were: (i) the histopathological index (HP) of the main organs: gills, liver and kidney; and (ii) the histomorphometric parameters of the hepatocytes and enterocytes to assess the state of the digestive system in fish fed different types of supplemented feed. At the end of the experiment, the fish were in good health, since irreversible lesions were not noticed to a greater extent in any of the fish organs. There were also no other severe lesions present. However, the results showed differences in the HP indices of the liver and gills, as well as the total HP index, indicating a better health status of the fish in the CF group. The histomorphometric results showed larger cytoplasmic areas of hepatocytes in fish fed CF, which is attributed to glycogen accumulation and higher numbers of goblet cells in the distal intestines of fish fed compound feeds. This can be attributed to slight inflammation.
PB  - Wiley, Hoboken
T2  - Aquaculture Research
T1  - Integrative approach of histopathology and histomorphometry of common carp (Cyprinus carpio L.) organs as a marker of general fish health state in pond culture
VL  - 47
IS  - 11
SP  - 3455
EP  - 3463
DO  - 10.1111/are.12795
ER  - 

@article{
author = "Raskovic, Bozidar and Cicovacki, Stanislav and Ćirić, Miloš and Markovic, Zoran and Poleksić, Vesna",
year = "2016",
abstract = "The semi-intensive system of common carp mono-culture in earthen ponds is currently the most practiced fish rearing system in several countries in Eastern Europe. This system is based on the addition of supplemented feed to ponds to achieve faster growth. In this study, the general state of the fish health was monitored when three different types of feeds were used: grain cereals (CF), commercial pelleted feed and extruded feed. The markers used were: (i) the histopathological index (HP) of the main organs: gills, liver and kidney; and (ii) the histomorphometric parameters of the hepatocytes and enterocytes to assess the state of the digestive system in fish fed different types of supplemented feed. At the end of the experiment, the fish were in good health, since irreversible lesions were not noticed to a greater extent in any of the fish organs. There were also no other severe lesions present. However, the results showed differences in the HP indices of the liver and gills, as well as the total HP index, indicating a better health status of the fish in the CF group. The histomorphometric results showed larger cytoplasmic areas of hepatocytes in fish fed CF, which is attributed to glycogen accumulation and higher numbers of goblet cells in the distal intestines of fish fed compound feeds. This can be attributed to slight inflammation.",
publisher = "Wiley, Hoboken",
journal = "Aquaculture Research",
title = "Integrative approach of histopathology and histomorphometry of common carp (Cyprinus carpio L.) organs as a marker of general fish health state in pond culture",
volume = "47",
number = "11",
pages = "3455-3463",
doi = "10.1111/are.12795"
}

Raskovic, B., Cicovacki, S., Ćirić, M., Markovic, Z.,& Poleksić, V.. (2016). Integrative approach of histopathology and histomorphometry of common carp (Cyprinus carpio L.) organs as a marker of general fish health state in pond culture. in Aquaculture Research
Wiley, Hoboken., 47(11), 3455-3463.
https://doi.org/10.1111/are.12795

Raskovic B, Cicovacki S, Ćirić M, Markovic Z, Poleksić V. Integrative approach of histopathology and histomorphometry of common carp (Cyprinus carpio L.) organs as a marker of general fish health state in pond culture. in Aquaculture Research. 2016;47(11):3455-3463.
doi:10.1111/are.12795 .

Raskovic, Bozidar, Cicovacki, Stanislav, Ćirić, Miloš, Markovic, Zoran, Poleksić, Vesna, "Integrative approach of histopathology and histomorphometry of common carp (Cyprinus carpio L.) organs as a marker of general fish health state in pond culture" in Aquaculture Research, 47, no. 11 (2016):3455-3463,
https://doi.org/10.1111/are.12795 . .

TY  - JOUR
AU  - Poleksić, Vesna
AU  - Lenhardt, Mirjana
AU  - Jaric, Ivan
AU  - Đorđević, Dragana
AU  - Gačić, Zoran
AU  - Cvijanovic, Gorcin
AU  - Raskovic, Bozidar
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/696
AB  - The sterlet (Acipenser ruthenus L.) is a bottom-feeding fish species with a direct exposure to contaminants from water and sediments. Although heavy metal pollution is believed to be one of the main threats to the sterlet population in the Danube River basin, there is a lack of knowledge of the exact impact of heavy metals on their survival. In the present study, effects of heavy metal pollution on sterlet in the Danube basin were assessed as well as the utility of different sterlet organs and tissues as indicators of heavy metal contamination. The sterlet were sampled at three different sites in the Danube basin, in Hungary and Serbia, isolated from each other by dams. Heavy metal analysis included measurement of Cd, As, Pb, Cr, Hg, Cu, Ni, Fe, Mn, and Zn concentrations in sterlet gills, muscle, liver, and intestine, and histopathological analyses comprised assessment and scoring of the extent and intensity of alterations in skin, gills, and liver tissue. Analysis revealed a significant presence of sublethal histopathological changes that were most pronounced in the liver and skin and increased accumulation of heavy metals, with the highest concentrations in the liver. Canonical discriminant analysis showed significant differentiation among the three studied localities, suggesting that the heavy metal concentrations in sterlet populations were site specific. The present study concludes that the accumulation of heavy metals is a response to the presence of these pollutants in the environment, and, together with other pollutants, it affects the vital organs of natural sterlet populations.
PB  - Setac Press, Pensacola
T2  - Environmental Toxicology and Chemistry
T1  - Liver, gills, and skin histopathology and heavy metal content of the danube sterlet (acipenser ruthenus linnaeus, 1758)
VL  - 29
IS  - 3
SP  - 515
EP  - 521
DO  - 10.1002/etc.82
ER  - 

@article{
author = "Poleksić, Vesna and Lenhardt, Mirjana and Jaric, Ivan and Đorđević, Dragana and Gačić, Zoran and Cvijanovic, Gorcin and Raskovic, Bozidar",
year = "2010",
abstract = "The sterlet (Acipenser ruthenus L.) is a bottom-feeding fish species with a direct exposure to contaminants from water and sediments. Although heavy metal pollution is believed to be one of the main threats to the sterlet population in the Danube River basin, there is a lack of knowledge of the exact impact of heavy metals on their survival. In the present study, effects of heavy metal pollution on sterlet in the Danube basin were assessed as well as the utility of different sterlet organs and tissues as indicators of heavy metal contamination. The sterlet were sampled at three different sites in the Danube basin, in Hungary and Serbia, isolated from each other by dams. Heavy metal analysis included measurement of Cd, As, Pb, Cr, Hg, Cu, Ni, Fe, Mn, and Zn concentrations in sterlet gills, muscle, liver, and intestine, and histopathological analyses comprised assessment and scoring of the extent and intensity of alterations in skin, gills, and liver tissue. Analysis revealed a significant presence of sublethal histopathological changes that were most pronounced in the liver and skin and increased accumulation of heavy metals, with the highest concentrations in the liver. Canonical discriminant analysis showed significant differentiation among the three studied localities, suggesting that the heavy metal concentrations in sterlet populations were site specific. The present study concludes that the accumulation of heavy metals is a response to the presence of these pollutants in the environment, and, together with other pollutants, it affects the vital organs of natural sterlet populations.",
publisher = "Setac Press, Pensacola",
journal = "Environmental Toxicology and Chemistry",
title = "Liver, gills, and skin histopathology and heavy metal content of the danube sterlet (acipenser ruthenus linnaeus, 1758)",
volume = "29",
number = "3",
pages = "515-521",
doi = "10.1002/etc.82"
}

Poleksić, V., Lenhardt, M., Jaric, I., Đorđević, D., Gačić, Z., Cvijanovic, G.,& Raskovic, B.. (2010). Liver, gills, and skin histopathology and heavy metal content of the danube sterlet (acipenser ruthenus linnaeus, 1758). in Environmental Toxicology and Chemistry
Setac Press, Pensacola., 29(3), 515-521.
https://doi.org/10.1002/etc.82

Poleksić V, Lenhardt M, Jaric I, Đorđević D, Gačić Z, Cvijanovic G, Raskovic B. Liver, gills, and skin histopathology and heavy metal content of the danube sterlet (acipenser ruthenus linnaeus, 1758). in Environmental Toxicology and Chemistry. 2010;29(3):515-521.
doi:10.1002/etc.82 .

Poleksić, Vesna, Lenhardt, Mirjana, Jaric, Ivan, Đorđević, Dragana, Gačić, Zoran, Cvijanovic, Gorcin, Raskovic, Bozidar, "Liver, gills, and skin histopathology and heavy metal content of the danube sterlet (acipenser ruthenus linnaeus, 1758)" in Environmental Toxicology and Chemistry, 29, no. 3 (2010):515-521,
https://doi.org/10.1002/etc.82 . .