TY  - JOUR
AU  - Miladinovic, Dragoljub L.
AU  - Ilic, Budimir S.
AU  - Mihajilov-Krstev, Tatjana M.
AU  - Nikolic, Dejan M.
AU  - Cvetković, Olga
AU  - Marković, Marija S.
AU  - Miladinovic, Ljiljana C.
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1265
AB  - The composition and antimicrobial activity of the essential oil of Heracleum sibiricum L. (Apiaceae) was studied. The aerial part of plant was hydro-distilled and chemical composition of the essential oil was analyzed by GC and GC-MS. Forty-six compounds, corresponding to 95.12% of the total oil, were identified. Esters represented the major chemical class (69.55%) while the main constituents were octyl butanoate (36.82%), hexyl butanoate (16.08%), 1-octanol (13.62%) and octyl hexanoate (8.10%). Antibacterial activity of the essential oil and reference antibiotics against nine bacterial strains was tested by the broth microdilution method. The results of the bioassays showed that essential oil had slight antimicrobial activities against all tested microorganisms (MIC and MBC values were in the range of 2431.2 to 9724.8 mu g/mL). Reference antibiotics were active in concentrations between 0.5 and 16.0 mu g/mL. The results confirm that Gram-positive bacteria were more susceptible to the essential oil of H. sibiricum, in comparison with Gram-negative bacteria.
PB  - Natural Product Incorporation
T2  - Natural Product Communications
T1  - Antibacterial Activity of the Essential Oil of Heracleum sibiricum
VL  - 8
IS  - 9
SP  - 1309
EP  - 1311
UR  - https://hdl.handle.net/21.15107/rcub_cer_1265
ER  - 

@article{
author = "Miladinovic, Dragoljub L. and Ilic, Budimir S. and Mihajilov-Krstev, Tatjana M. and Nikolic, Dejan M. and Cvetković, Olga and Marković, Marija S. and Miladinovic, Ljiljana C.",
year = "2013",
abstract = "The composition and antimicrobial activity of the essential oil of Heracleum sibiricum L. (Apiaceae) was studied. The aerial part of plant was hydro-distilled and chemical composition of the essential oil was analyzed by GC and GC-MS. Forty-six compounds, corresponding to 95.12% of the total oil, were identified. Esters represented the major chemical class (69.55%) while the main constituents were octyl butanoate (36.82%), hexyl butanoate (16.08%), 1-octanol (13.62%) and octyl hexanoate (8.10%). Antibacterial activity of the essential oil and reference antibiotics against nine bacterial strains was tested by the broth microdilution method. The results of the bioassays showed that essential oil had slight antimicrobial activities against all tested microorganisms (MIC and MBC values were in the range of 2431.2 to 9724.8 mu g/mL). Reference antibiotics were active in concentrations between 0.5 and 16.0 mu g/mL. The results confirm that Gram-positive bacteria were more susceptible to the essential oil of H. sibiricum, in comparison with Gram-negative bacteria.",
publisher = "Natural Product Incorporation",
journal = "Natural Product Communications",
title = "Antibacterial Activity of the Essential Oil of Heracleum sibiricum",
volume = "8",
number = "9",
pages = "1309-1311",
url = "https://hdl.handle.net/21.15107/rcub_cer_1265"
}

Miladinovic, D. L., Ilic, B. S., Mihajilov-Krstev, T. M., Nikolic, D. M., Cvetković, O., Marković, M. S.,& Miladinovic, L. C.. (2013). Antibacterial Activity of the Essential Oil of Heracleum sibiricum. in Natural Product Communications
Natural Product Incorporation., 8(9), 1309-1311.
https://hdl.handle.net/21.15107/rcub_cer_1265

Miladinovic DL, Ilic BS, Mihajilov-Krstev TM, Nikolic DM, Cvetković O, Marković MS, Miladinovic LC. Antibacterial Activity of the Essential Oil of Heracleum sibiricum. in Natural Product Communications. 2013;8(9):1309-1311.
https://hdl.handle.net/21.15107/rcub_cer_1265 .

Miladinovic, Dragoljub L., Ilic, Budimir S., Mihajilov-Krstev, Tatjana M., Nikolic, Dejan M., Cvetković, Olga, Marković, Marija S., Miladinovic, Ljiljana C., "Antibacterial Activity of the Essential Oil of Heracleum sibiricum" in Natural Product Communications, 8, no. 9 (2013):1309-1311,
https://hdl.handle.net/21.15107/rcub_cer_1265 .

TY  - JOUR
AU  - Miladinovic, Dragoljub L.
AU  - Ilic, Budimir S.
AU  - Mihajilov-Krstev, Tatjana M.
AU  - Nikolic, Nikola D.
AU  - Miladinovic, Ljiljana C.
AU  - Cvetković, Olga
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/954
AB  - The antibacterial effects of Thymus vulgaris (Lamiaceae), Lavandula angustifolia (Lamiaceae), and Calamintha nepeta (Lamiaceae) Savi subsp. nepeta var. subisodonda (Borb.) Hayek essential oils on five different bacteria were estimated. Laboratory control strain and clinical isolates from different pathogenic media were researched by broth microdilution method, with an emphasis on a chemical composition-antibacterial activity relationship. The main constituents of thyme oil were thymol (59.95%) and p-cymene (18.34%). Linalool acetate (38.23%) and beta-linalool (35.01%) were main compounds in lavender oil. C. nepeta essential oil was characterized by a high percentage of piperitone oxide (59.07%) and limonene (9.05%). Essential oils have been found to have antimicrobial activity against all tested microorganisms. Classification and comparison of essential oils on the basis of their chemical composition and antibacterial activity were made by utilization of appropriate chemometric methods. The chemical principal component analysis (PCA) and hierachical cluster analysis (HCA) separated essential oils into two groups and two sub-groups. Thyme essential oil forms separate chemical HCA group and exhibits highest antibacterial activity, similar to tetracycline. Essential oils of lavender and C. nepeta in the same chemical HCA group were classified in different groups, within antibacterial PCA and HCA analyses. Lavender oil exhibits higher antibacterial ability in comparison with C. nepeta essential oil, probably based on the concept of synergistic activity of essential oil components.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods
VL  - 403
IS  - 4
SP  - 1007
EP  - 1018
DO  - 10.1007/s00216-012-5866-1
ER  - 

@article{
author = "Miladinovic, Dragoljub L. and Ilic, Budimir S. and Mihajilov-Krstev, Tatjana M. and Nikolic, Nikola D. and Miladinovic, Ljiljana C. and Cvetković, Olga",
year = "2012",
abstract = "The antibacterial effects of Thymus vulgaris (Lamiaceae), Lavandula angustifolia (Lamiaceae), and Calamintha nepeta (Lamiaceae) Savi subsp. nepeta var. subisodonda (Borb.) Hayek essential oils on five different bacteria were estimated. Laboratory control strain and clinical isolates from different pathogenic media were researched by broth microdilution method, with an emphasis on a chemical composition-antibacterial activity relationship. The main constituents of thyme oil were thymol (59.95%) and p-cymene (18.34%). Linalool acetate (38.23%) and beta-linalool (35.01%) were main compounds in lavender oil. C. nepeta essential oil was characterized by a high percentage of piperitone oxide (59.07%) and limonene (9.05%). Essential oils have been found to have antimicrobial activity against all tested microorganisms. Classification and comparison of essential oils on the basis of their chemical composition and antibacterial activity were made by utilization of appropriate chemometric methods. The chemical principal component analysis (PCA) and hierachical cluster analysis (HCA) separated essential oils into two groups and two sub-groups. Thyme essential oil forms separate chemical HCA group and exhibits highest antibacterial activity, similar to tetracycline. Essential oils of lavender and C. nepeta in the same chemical HCA group were classified in different groups, within antibacterial PCA and HCA analyses. Lavender oil exhibits higher antibacterial ability in comparison with C. nepeta essential oil, probably based on the concept of synergistic activity of essential oil components.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods",
volume = "403",
number = "4",
pages = "1007-1018",
doi = "10.1007/s00216-012-5866-1"
}

Miladinovic, D. L., Ilic, B. S., Mihajilov-Krstev, T. M., Nikolic, N. D., Miladinovic, L. C.,& Cvetković, O.. (2012). Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 403(4), 1007-1018.
https://doi.org/10.1007/s00216-012-5866-1

Miladinovic DL, Ilic BS, Mihajilov-Krstev TM, Nikolic ND, Miladinovic LC, Cvetković O. Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods. in Analytical and Bioanalytical Chemistry. 2012;403(4):1007-1018.
doi:10.1007/s00216-012-5866-1 .

Miladinovic, Dragoljub L., Ilic, Budimir S., Mihajilov-Krstev, Tatjana M., Nikolic, Nikola D., Miladinovic, Ljiljana C., Cvetković, Olga, "Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods" in Analytical and Bioanalytical Chemistry, 403, no. 4 (2012):1007-1018,
https://doi.org/10.1007/s00216-012-5866-1 . .