TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Malagurski, Ivana
AU  - Lazić, Jelena
AU  - Jeremić, Sanja
AU  - Pavlović, Vladimir
AU  - Prlainović, Nevena
AU  - Maksimović, Vesna
AU  - Ćosović, Vladan
AU  - Atanase, Leonard Ionut
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5690
AB  - The quest for sustainable biomaterials with excellent biocompatibility and tailorable
properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production
costs and the lack of bioactivity limit their market penetration. To address this, poly(3-
hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong
anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The
samples were produced in the form of films 115.6–118.8  m in thickness using the solvent casting
method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity
and thermal stability) and functionality of the obtained biomaterials were investigated. PG
has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and
morphology of the films. All samples with PG had a more organized internal structure and higher
melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer
was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.
Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon
cancer) cells, thus advancing PHBV biomedical application potential.
PB  - Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T1  - Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin
VL  - 24
SP  - 1906
DO  - 10.3390/ijms24031906
ER  - 

@article{
author = "Ponjavić, Marijana and Malagurski, Ivana and Lazić, Jelena and Jeremić, Sanja and Pavlović, Vladimir and Prlainović, Nevena and Maksimović, Vesna and Ćosović, Vladan and Atanase, Leonard Ionut",
year = "2023",
abstract = "The quest for sustainable biomaterials with excellent biocompatibility and tailorable
properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production
costs and the lack of bioactivity limit their market penetration. To address this, poly(3-
hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong
anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The
samples were produced in the form of films 115.6–118.8  m in thickness using the solvent casting
method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity
and thermal stability) and functionality of the obtained biomaterials were investigated. PG
has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and
morphology of the films. All samples with PG had a more organized internal structure and higher
melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer
was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.
Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon
cancer) cells, thus advancing PHBV biomedical application potential.",
publisher = "Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences",
title = "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin",
volume = "24",
pages = "1906",
doi = "10.3390/ijms24031906"
}

Ponjavić, M., Malagurski, I., Lazić, J., Jeremić, S., Pavlović, V., Prlainović, N., Maksimović, V., Ćosović, V.,& Atanase, L. I.. (2023). Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences
Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)., 24, 1906.
https://doi.org/10.3390/ijms24031906

Ponjavić M, Malagurski I, Lazić J, Jeremić S, Pavlović V, Prlainović N, Maksimović V, Ćosović V, Atanase LI. Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences. 2023;24:1906.
doi:10.3390/ijms24031906 .

Ponjavić, Marijana, Malagurski, Ivana, Lazić, Jelena, Jeremić, Sanja, Pavlović, Vladimir, Prlainović, Nevena, Maksimović, Vesna, Ćosović, Vladan, Atanase, Leonard Ionut, "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin" in International Journal of Molecular Sciences, 24 (2023):1906,
https://doi.org/10.3390/ijms24031906 . .

TY  - CONF
AU  - Milošević, Milena
AU  - Cvijetić, Ilija
AU  - Božić, Aleksandra
AU  - Prlainović, Nevena
AU  - Bjelogrlić, Snežana
AU  - Popović, Mina
AU  - Marinković, Aleksandar
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6963
AB  - Thiocarbohydrazones and their derivatives represent a class of compounds with various biological and pharmaceutical properties, including strong antioxidant, antitubercular, antimicrobial, and anticancer activity [1]. Therefore, in this work, new asymmetrically substituted bis-(thiocarbohydrazones) (TCHs) bearing 2-pyridine and quinoline moiety were synthesized and showed promising in vitro antioxidant and anticancer activity (Figure1). The results suggest that antioxidant activity of TCH depends on the structure, substituent type and antioxidant assay used. The maximum antioxidant activity in DPPH and CUPRAC tests was observed for compound with 8-quinolyl and 8-hydroxy-2quinolyl moiety. Additionally, anticancer assays revealed that compounds interfere with cancer cell mobility at concentrations below 10 μM, and exert low toxicity toward healthy human HaCaT fibroblasts. The results of this study represent a good foundation for further research and development of novel iminopyridines with improved antioxidant and anticancer activity.
PB  - Hellenic Society of Medicinal Chemistry (HSMC)
C3  - 18th Hellenic Symposium on Medicinal Chemistry, on-line, Greece, 25-27 February 2021
T1  - Experimental study of antioxidant and anticancer activity of new asymmetrically substituted thiocarbohydrazones
SP  - P065
UR  - https://hdl.handle.net/21.15107/rcub_cer_6963
ER  - 

@conference{
author = "Milošević, Milena and Cvijetić, Ilija and Božić, Aleksandra and Prlainović, Nevena and Bjelogrlić, Snežana and Popović, Mina and Marinković, Aleksandar",
year = "2021",
abstract = "Thiocarbohydrazones and their derivatives represent a class of compounds with various biological and pharmaceutical properties, including strong antioxidant, antitubercular, antimicrobial, and anticancer activity [1]. Therefore, in this work, new asymmetrically substituted bis-(thiocarbohydrazones) (TCHs) bearing 2-pyridine and quinoline moiety were synthesized and showed promising in vitro antioxidant and anticancer activity (Figure1). The results suggest that antioxidant activity of TCH depends on the structure, substituent type and antioxidant assay used. The maximum antioxidant activity in DPPH and CUPRAC tests was observed for compound with 8-quinolyl and 8-hydroxy-2quinolyl moiety. Additionally, anticancer assays revealed that compounds interfere with cancer cell mobility at concentrations below 10 μM, and exert low toxicity toward healthy human HaCaT fibroblasts. The results of this study represent a good foundation for further research and development of novel iminopyridines with improved antioxidant and anticancer activity.",
publisher = "Hellenic Society of Medicinal Chemistry (HSMC)",
journal = "18th Hellenic Symposium on Medicinal Chemistry, on-line, Greece, 25-27 February 2021",
title = "Experimental study of antioxidant and anticancer activity of new asymmetrically substituted thiocarbohydrazones",
pages = "P065",
url = "https://hdl.handle.net/21.15107/rcub_cer_6963"
}

Milošević, M., Cvijetić, I., Božić, A., Prlainović, N., Bjelogrlić, S., Popović, M.,& Marinković, A.. (2021). Experimental study of antioxidant and anticancer activity of new asymmetrically substituted thiocarbohydrazones. in 18th Hellenic Symposium on Medicinal Chemistry, on-line, Greece, 25-27 February 2021
Hellenic Society of Medicinal Chemistry (HSMC)., P065.
https://hdl.handle.net/21.15107/rcub_cer_6963

Milošević M, Cvijetić I, Božić A, Prlainović N, Bjelogrlić S, Popović M, Marinković A. Experimental study of antioxidant and anticancer activity of new asymmetrically substituted thiocarbohydrazones. in 18th Hellenic Symposium on Medicinal Chemistry, on-line, Greece, 25-27 February 2021. 2021;:P065.
https://hdl.handle.net/21.15107/rcub_cer_6963 .

Milošević, Milena, Cvijetić, Ilija, Božić, Aleksandra, Prlainović, Nevena, Bjelogrlić, Snežana, Popović, Mina, Marinković, Aleksandar, "Experimental study of antioxidant and anticancer activity of new asymmetrically substituted thiocarbohydrazones" in 18th Hellenic Symposium on Medicinal Chemistry, on-line, Greece, 25-27 February 2021 (2021):P065,
https://hdl.handle.net/21.15107/rcub_cer_6963 .

TY  - JOUR
AU  - Milošević, Milena D.
AU  - Marinković, Aleksandar D.
AU  - Petrović, Predrag
AU  - Klaus, Anita
AU  - Nikolić, Milica G.
AU  - Prlainović, Nevena Ž.
AU  - Cvijetić, Ilija
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3627
AB  - In this study we synthesized a series of sixteen bis(imino)pyridines (BIPs) starting from 2,6-diaminopyridine and various aromatic aldehydes, and evaluated their antioxidant, antibacterial, antifungal and acetylcholinesterase (AChE) inhibitory activity. The chemical structures were elucidated by FTIR, elemental analysis, ESR and HRMS. 1H and 13C NMR spectra couldn’t be acquired due to the formation of stable, carbon-centered radical cations in a solution, as confirmed by ESR spectroscopy and DFT calculations. The in vitro antioxidant potency was evaluated using four assays: free radical scavenging activity (DPPH and ABTS), reducing power and total antioxidant capacity assay. BIPs demonstrated excellent antioxidant properties, and two derivatives proved to be more potent than reference antioxidants (ascorbic acid and Trolox) in all assays. DFT calculations on ωB97XD/6-311++g(d,p) level of theory provided valuable insights into the radical scavenging mechanism of BIPs. For hydroxyl-substituted BIPs, hydrogen atom transfer (HAT) is a predominant mechanism, while the single electron transfer coupled with proton transfer (SET-PT) governs the antioxidant activity of other derivatives. Intramolecular hydrogen bonding (IHB) plays an important role in the mechanism of antioxidant activity as revealed by noncovalent interaction analysis and rotational barrier calculations. The spin density of radical cations is localized on carbon atoms of a pyridine ring, which corroborates with g-factors and multiplicity obtained from ESR analysis. The most potent BIP exhibited moderate inhibitory activity toward AChE (IC50=20 ± 4 μM), while molecular docking suggested binding at the peripheral anionic site of AChE with the MMFF94 binding enthalpy of −43.4 kcal/mol. Moderate in vitro antimicrobial activity of BIPs have been determined against several pathogenic bacterial strains: Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and clinical isolate of methicillin resistant S. aureus (MRSA). The antifungal activity of BIPs toward Candida albicans was also confirmed. The similarity ensemble approach combined with molecular docking suggested leucyl aminopeptidase as the probable antimicrobial target for the three most potent BIP derivatives.
PB  - Elsevier
T2  - Bioorganic Chemistry
T1  - Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents
VL  - 102
SP  - 104073
DO  - 10.1016/j.bioorg.2020.104073
ER  - 

@article{
author = "Milošević, Milena D. and Marinković, Aleksandar D. and Petrović, Predrag and Klaus, Anita and Nikolić, Milica G. and Prlainović, Nevena Ž. and Cvijetić, Ilija",
year = "2020",
abstract = "In this study we synthesized a series of sixteen bis(imino)pyridines (BIPs) starting from 2,6-diaminopyridine and various aromatic aldehydes, and evaluated their antioxidant, antibacterial, antifungal and acetylcholinesterase (AChE) inhibitory activity. The chemical structures were elucidated by FTIR, elemental analysis, ESR and HRMS. 1H and 13C NMR spectra couldn’t be acquired due to the formation of stable, carbon-centered radical cations in a solution, as confirmed by ESR spectroscopy and DFT calculations. The in vitro antioxidant potency was evaluated using four assays: free radical scavenging activity (DPPH and ABTS), reducing power and total antioxidant capacity assay. BIPs demonstrated excellent antioxidant properties, and two derivatives proved to be more potent than reference antioxidants (ascorbic acid and Trolox) in all assays. DFT calculations on ωB97XD/6-311++g(d,p) level of theory provided valuable insights into the radical scavenging mechanism of BIPs. For hydroxyl-substituted BIPs, hydrogen atom transfer (HAT) is a predominant mechanism, while the single electron transfer coupled with proton transfer (SET-PT) governs the antioxidant activity of other derivatives. Intramolecular hydrogen bonding (IHB) plays an important role in the mechanism of antioxidant activity as revealed by noncovalent interaction analysis and rotational barrier calculations. The spin density of radical cations is localized on carbon atoms of a pyridine ring, which corroborates with g-factors and multiplicity obtained from ESR analysis. The most potent BIP exhibited moderate inhibitory activity toward AChE (IC50=20 ± 4 μM), while molecular docking suggested binding at the peripheral anionic site of AChE with the MMFF94 binding enthalpy of −43.4 kcal/mol. Moderate in vitro antimicrobial activity of BIPs have been determined against several pathogenic bacterial strains: Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and clinical isolate of methicillin resistant S. aureus (MRSA). The antifungal activity of BIPs toward Candida albicans was also confirmed. The similarity ensemble approach combined with molecular docking suggested leucyl aminopeptidase as the probable antimicrobial target for the three most potent BIP derivatives.",
publisher = "Elsevier",
journal = "Bioorganic Chemistry",
title = "Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents",
volume = "102",
pages = "104073",
doi = "10.1016/j.bioorg.2020.104073"
}

Milošević, M. D., Marinković, A. D., Petrović, P., Klaus, A., Nikolić, M. G., Prlainović, N. Ž.,& Cvijetić, I.. (2020). Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. in Bioorganic Chemistry
Elsevier., 102, 104073.
https://doi.org/10.1016/j.bioorg.2020.104073

Milošević MD, Marinković AD, Petrović P, Klaus A, Nikolić MG, Prlainović NŽ, Cvijetić I. Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. in Bioorganic Chemistry. 2020;102:104073.
doi:10.1016/j.bioorg.2020.104073 .

Milošević, Milena D., Marinković, Aleksandar D., Petrović, Predrag, Klaus, Anita, Nikolić, Milica G., Prlainović, Nevena Ž., Cvijetić, Ilija, "Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents" in Bioorganic Chemistry, 102 (2020):104073,
https://doi.org/10.1016/j.bioorg.2020.104073 . .

TY  - JOUR
AU  - Milošević, Milena D.
AU  - Marinković, Aleksandar D.
AU  - Petrović, Predrag
AU  - Klaus, Anita
AU  - Nikolić, Milica G.
AU  - Prlainović, Nevena Ž.
AU  - Cvijetić, Ilija
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3630
AB  - In this study we synthesized a series of sixteen bis(imino)pyridines (BIPs) starting from 2,6-diaminopyridine and various aromatic aldehydes, and evaluated their antioxidant, antibacterial, antifungal and acetylcholinesterase (AChE) inhibitory activity. The chemical structures were elucidated by FTIR, elemental analysis, ESR and HRMS. 1H and 13C NMR spectra couldn’t be acquired due to the formation of stable, carbon-centered radical cations in a solution, as confirmed by ESR spectroscopy and DFT calculations. The in vitro antioxidant potency was evaluated using four assays: free radical scavenging activity (DPPH and ABTS), reducing power and total antioxidant capacity assay. BIPs demonstrated excellent antioxidant properties, and two derivatives proved to be more potent than reference antioxidants (ascorbic acid and Trolox) in all assays. DFT calculations on ωB97XD/6-311++g(d,p) level of theory provided valuable insights into the radical scavenging mechanism of BIPs. For hydroxyl-substituted BIPs, hydrogen atom transfer (HAT) is a predominant mechanism, while the single electron transfer coupled with proton transfer (SET-PT) governs the antioxidant activity of other derivatives. Intramolecular hydrogen bonding (IHB) plays an important role in the mechanism of antioxidant activity as revealed by noncovalent interaction analysis and rotational barrier calculations. The spin density of radical cations is localized on carbon atoms of a pyridine ring, which corroborates with g-factors and multiplicity obtained from ESR analysis. The most potent BIP exhibited moderate inhibitory activity toward AChE (IC50=20 ± 4 μM), while molecular docking suggested binding at the peripheral anionic site of AChE with theMMFF94 binding enthalpy of −43.4 kcal/mol. Moderate in vitro antimicrobial activity of BIPs have been determined against several pathogenic bacterial strains: Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and clinical isolate of methicillin resistant S. aureus (MRSA). The antifungal activity of BIPs toward Candida albicans was also confirmed. The similarity ensemble approach combined with molecular docking suggested leucyl aminopeptidase as the probable antimicrobial target for the three most potent BIP derivatives.
PB  - Elsevier
T2  - Bioorganic Chemistry
T1  - Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents
VL  - 102
SP  - 104073
DO  - 10.1016/j.bioorg.2020.104073
ER  - 

@article{
author = "Milošević, Milena D. and Marinković, Aleksandar D. and Petrović, Predrag and Klaus, Anita and Nikolić, Milica G. and Prlainović, Nevena Ž. and Cvijetić, Ilija",
year = "2020",
abstract = "In this study we synthesized a series of sixteen bis(imino)pyridines (BIPs) starting from 2,6-diaminopyridine and various aromatic aldehydes, and evaluated their antioxidant, antibacterial, antifungal and acetylcholinesterase (AChE) inhibitory activity. The chemical structures were elucidated by FTIR, elemental analysis, ESR and HRMS. 1H and 13C NMR spectra couldn’t be acquired due to the formation of stable, carbon-centered radical cations in a solution, as confirmed by ESR spectroscopy and DFT calculations. The in vitro antioxidant potency was evaluated using four assays: free radical scavenging activity (DPPH and ABTS), reducing power and total antioxidant capacity assay. BIPs demonstrated excellent antioxidant properties, and two derivatives proved to be more potent than reference antioxidants (ascorbic acid and Trolox) in all assays. DFT calculations on ωB97XD/6-311++g(d,p) level of theory provided valuable insights into the radical scavenging mechanism of BIPs. For hydroxyl-substituted BIPs, hydrogen atom transfer (HAT) is a predominant mechanism, while the single electron transfer coupled with proton transfer (SET-PT) governs the antioxidant activity of other derivatives. Intramolecular hydrogen bonding (IHB) plays an important role in the mechanism of antioxidant activity as revealed by noncovalent interaction analysis and rotational barrier calculations. The spin density of radical cations is localized on carbon atoms of a pyridine ring, which corroborates with g-factors and multiplicity obtained from ESR analysis. The most potent BIP exhibited moderate inhibitory activity toward AChE (IC50=20 ± 4 μM), while molecular docking suggested binding at the peripheral anionic site of AChE with theMMFF94 binding enthalpy of −43.4 kcal/mol. Moderate in vitro antimicrobial activity of BIPs have been determined against several pathogenic bacterial strains: Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and clinical isolate of methicillin resistant S. aureus (MRSA). The antifungal activity of BIPs toward Candida albicans was also confirmed. The similarity ensemble approach combined with molecular docking suggested leucyl aminopeptidase as the probable antimicrobial target for the three most potent BIP derivatives.",
publisher = "Elsevier",
journal = "Bioorganic Chemistry",
title = "Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents",
volume = "102",
pages = "104073",
doi = "10.1016/j.bioorg.2020.104073"
}

Milošević, M. D., Marinković, A. D., Petrović, P., Klaus, A., Nikolić, M. G., Prlainović, N. Ž.,& Cvijetić, I.. (2020). Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. in Bioorganic Chemistry
Elsevier., 102, 104073.
https://doi.org/10.1016/j.bioorg.2020.104073

Milošević MD, Marinković AD, Petrović P, Klaus A, Nikolić MG, Prlainović NŽ, Cvijetić I. Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. in Bioorganic Chemistry. 2020;102:104073.
doi:10.1016/j.bioorg.2020.104073 .

Milošević, Milena D., Marinković, Aleksandar D., Petrović, Predrag, Klaus, Anita, Nikolić, Milica G., Prlainović, Nevena Ž., Cvijetić, Ilija, "Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents" in Bioorganic Chemistry, 102 (2020):104073,
https://doi.org/10.1016/j.bioorg.2020.104073 . .

TY  - JOUR
AU  - Milošević, Milena
AU  - Prlainović, Nevena
AU  - Milčić, Miloš
AU  - Nikolić, Vesna
AU  - Božić, Aleksandra
AU  - Bigović, Miljan
AU  - Marinković, Aleksandar
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6959
AB  - 15 symmetric 1-methyl-2,6-bis[2-(substituted phenyl)ethenyl]pyridinium iodides were synthesized in this work. Their structures were characterized using IR, H-1 and C-13 NMR, and UV-Vis spectroscopy. DFT calculations indicated that s-trans/s-trans conformation prevail in all compounds. The effects of specific and non-specific solvent-solute interactions on the UV-Vis absorption maxima shifts were evaluated using linear solvation-free energy relationships (LSER), i.e., Kamlet-Taft and Catalan models. A linear free energy relationship (LFER) in the form of single substituent parameter equations (SSP) was used to postulate quantitative structure-property relations of substituent effect on NMR data. TD-DFT results showed dependence of electronic transition on the substituent effects. The push-pull character of these compounds was analyzed by differences in C-13 chemical shift of the ethylenic double bond in 2- and 6-positions of cross-conjugated with pyridinum central ring. Also, the quotient of the occupations for the bonding pi and anti-bonding pi* orbitals of this bond was considered. Good correlations of the selected parameter between double bond lengths with pi*/pi and C-13 chemical shift differences of the bridging group proved them to be adequate descriptor of push-pull character. Synthesized compounds were screened for the antioxidant activity, using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical methods, and results demonstrated moderate antioxidant potential.
PB  - Springer
T2  - Journal of the Iranian Chemical Society
T1  - Solvent, structural, quantum chemical study and antioxidative activity of symmetrical 1-methyl-2,6-bis[2-(substituted phenyl)ethenyl]pyridinium iodides
VL  - 15
IS  - 11
SP  - 2483
EP  - 2501
DO  - 10.1007/s13738-018-1437-5
ER  - 

@article{
author = "Milošević, Milena and Prlainović, Nevena and Milčić, Miloš and Nikolić, Vesna and Božić, Aleksandra and Bigović, Miljan and Marinković, Aleksandar",
year = "2018",
abstract = "15 symmetric 1-methyl-2,6-bis[2-(substituted phenyl)ethenyl]pyridinium iodides were synthesized in this work. Their structures were characterized using IR, H-1 and C-13 NMR, and UV-Vis spectroscopy. DFT calculations indicated that s-trans/s-trans conformation prevail in all compounds. The effects of specific and non-specific solvent-solute interactions on the UV-Vis absorption maxima shifts were evaluated using linear solvation-free energy relationships (LSER), i.e., Kamlet-Taft and Catalan models. A linear free energy relationship (LFER) in the form of single substituent parameter equations (SSP) was used to postulate quantitative structure-property relations of substituent effect on NMR data. TD-DFT results showed dependence of electronic transition on the substituent effects. The push-pull character of these compounds was analyzed by differences in C-13 chemical shift of the ethylenic double bond in 2- and 6-positions of cross-conjugated with pyridinum central ring. Also, the quotient of the occupations for the bonding pi and anti-bonding pi* orbitals of this bond was considered. Good correlations of the selected parameter between double bond lengths with pi*/pi and C-13 chemical shift differences of the bridging group proved them to be adequate descriptor of push-pull character. Synthesized compounds were screened for the antioxidant activity, using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical methods, and results demonstrated moderate antioxidant potential.",
publisher = "Springer",
journal = "Journal of the Iranian Chemical Society",
title = "Solvent, structural, quantum chemical study and antioxidative activity of symmetrical 1-methyl-2,6-bis[2-(substituted phenyl)ethenyl]pyridinium iodides",
volume = "15",
number = "11",
pages = "2483-2501",
doi = "10.1007/s13738-018-1437-5"
}

Milošević, M., Prlainović, N., Milčić, M., Nikolić, V., Božić, A., Bigović, M.,& Marinković, A.. (2018). Solvent, structural, quantum chemical study and antioxidative activity of symmetrical 1-methyl-2,6-bis[2-(substituted phenyl)ethenyl]pyridinium iodides. in Journal of the Iranian Chemical Society
Springer., 15(11), 2483-2501.
https://doi.org/10.1007/s13738-018-1437-5

Milošević M, Prlainović N, Milčić M, Nikolić V, Božić A, Bigović M, Marinković A. Solvent, structural, quantum chemical study and antioxidative activity of symmetrical 1-methyl-2,6-bis[2-(substituted phenyl)ethenyl]pyridinium iodides. in Journal of the Iranian Chemical Society. 2018;15(11):2483-2501.
doi:10.1007/s13738-018-1437-5 .

Milošević, Milena, Prlainović, Nevena, Milčić, Miloš, Nikolić, Vesna, Božić, Aleksandra, Bigović, Miljan, Marinković, Aleksandar, "Solvent, structural, quantum chemical study and antioxidative activity of symmetrical 1-methyl-2,6-bis[2-(substituted phenyl)ethenyl]pyridinium iodides" in Journal of the Iranian Chemical Society, 15, no. 11 (2018):2483-2501,
https://doi.org/10.1007/s13738-018-1437-5 . .

TY  - JOUR
AU  - Lović, Jelena
AU  - Stevanović, Sanja
AU  - Anđelković, Boban D.
AU  - Petrovic, S.
AU  - Vukovic, D.
AU  - Prlainović, Nevena Ž.
AU  - Mijin, Dušan
AU  - Nikolić, Nebojša D.
AU  - Avramov Ivić, Milka
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2364
AB  - Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.
PB  - Esg, Belgrade
T2  - International Journal of Electrochemical Science
T1  - Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode
VL  - 13
IS  - 12
SP  - 12340
EP  - 12348
DO  - 10.20964/2018.12.59
ER  - 

@article{
author = "Lović, Jelena and Stevanović, Sanja and Anđelković, Boban D. and Petrovic, S. and Vukovic, D. and Prlainović, Nevena Ž. and Mijin, Dušan and Nikolić, Nebojša D. and Avramov Ivić, Milka",
year = "2018",
abstract = "Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.",
publisher = "Esg, Belgrade",
journal = "International Journal of Electrochemical Science",
title = "Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode",
volume = "13",
number = "12",
pages = "12340-12348",
doi = "10.20964/2018.12.59"
}

Lović, J., Stevanović, S., Anđelković, B. D., Petrovic, S., Vukovic, D., Prlainović, N. Ž., Mijin, D., Nikolić, N. D.,& Avramov Ivić, M.. (2018). Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode. in International Journal of Electrochemical Science
Esg, Belgrade., 13(12), 12340-12348.
https://doi.org/10.20964/2018.12.59

Lović J, Stevanović S, Anđelković BD, Petrovic S, Vukovic D, Prlainović NŽ, Mijin D, Nikolić ND, Avramov Ivić M. Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode. in International Journal of Electrochemical Science. 2018;13(12):12340-12348.
doi:10.20964/2018.12.59 .

Lović, Jelena, Stevanović, Sanja, Anđelković, Boban D., Petrovic, S., Vukovic, D., Prlainović, Nevena Ž., Mijin, Dušan, Nikolić, Nebojša D., Avramov Ivić, Milka, "Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode" in International Journal of Electrochemical Science, 13, no. 12 (2018):12340-12348,
https://doi.org/10.20964/2018.12.59 . .

TY  - JOUR
AU  - Lović, Jelena
AU  - Stevanović, Sanja
AU  - Nikolić, Nebojša D.
AU  - Petrovic, S.
AU  - Vukovic, D.
AU  - Prlainović, Nevena Ž.
AU  - Mijin, Dušan
AU  - Avramov Ivić, Milka
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2141
AB  - The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.
PB  - Esg, Belgrade
T2  - International Journal of Electrochemical Science
T1  - Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode
VL  - 12
IS  - 7
SP  - 5806
EP  - 5817
DO  - 10.20964/2017.07.65
ER  - 

@article{
author = "Lović, Jelena and Stevanović, Sanja and Nikolić, Nebojša D. and Petrovic, S. and Vukovic, D. and Prlainović, Nevena Ž. and Mijin, Dušan and Avramov Ivić, Milka",
year = "2017",
abstract = "The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.",
publisher = "Esg, Belgrade",
journal = "International Journal of Electrochemical Science",
title = "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode",
volume = "12",
number = "7",
pages = "5806-5817",
doi = "10.20964/2017.07.65"
}

Lović, J., Stevanović, S., Nikolić, N. D., Petrovic, S., Vukovic, D., Prlainović, N. Ž., Mijin, D.,& Avramov Ivić, M.. (2017). Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. in International Journal of Electrochemical Science
Esg, Belgrade., 12(7), 5806-5817.
https://doi.org/10.20964/2017.07.65

Lović J, Stevanović S, Nikolić ND, Petrovic S, Vukovic D, Prlainović NŽ, Mijin D, Avramov Ivić M. Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. in International Journal of Electrochemical Science. 2017;12(7):5806-5817.
doi:10.20964/2017.07.65 .

Lović, Jelena, Stevanović, Sanja, Nikolić, Nebojša D., Petrovic, S., Vukovic, D., Prlainović, Nevena Ž., Mijin, Dušan, Avramov Ivić, Milka, "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode" in International Journal of Electrochemical Science, 12, no. 7 (2017):5806-5817,
https://doi.org/10.20964/2017.07.65 . .

TY  - JOUR
AU  - Prlainović, Nevena Ž.
AU  - Bezbradica, Dejan I.
AU  - Knežević-Jugović, Zorica
AU  - Stevanović, Sanja
AU  - Avramov Ivić, Milka
AU  - Uskoković, Petar S.
AU  - Mijin, Dušan
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1297
AB  - In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.
PB  - Korean Society of Industrial Engineering Chemistry
T2  - Journal of Industrial and Engineering Chemistry
T1  - Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes
VL  - 19
IS  - 1
SP  - 279
EP  - 285
DO  - 10.1016/j.jiec.2012.08.012
ER  - 

@article{
author = "Prlainović, Nevena Ž. and Bezbradica, Dejan I. and Knežević-Jugović, Zorica and Stevanović, Sanja and Avramov Ivić, Milka and Uskoković, Petar S. and Mijin, Dušan",
year = "2013",
abstract = "In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.",
publisher = "Korean Society of Industrial Engineering Chemistry",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes",
volume = "19",
number = "1",
pages = "279-285",
doi = "10.1016/j.jiec.2012.08.012"
}

Prlainović, N. Ž., Bezbradica, D. I., Knežević-Jugović, Z., Stevanović, S., Avramov Ivić, M., Uskoković, P. S.,& Mijin, D.. (2013). Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry
Korean Society of Industrial Engineering Chemistry., 19(1), 279-285.
https://doi.org/10.1016/j.jiec.2012.08.012

Prlainović NŽ, Bezbradica DI, Knežević-Jugović Z, Stevanović S, Avramov Ivić M, Uskoković PS, Mijin D. Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry. 2013;19(1):279-285.
doi:10.1016/j.jiec.2012.08.012 .

Prlainović, Nevena Ž., Bezbradica, Dejan I., Knežević-Jugović, Zorica, Stevanović, Sanja, Avramov Ivić, Milka, Uskoković, Petar S., Mijin, Dušan, "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes" in Journal of Industrial and Engineering Chemistry, 19, no. 1 (2013):279-285,
https://doi.org/10.1016/j.jiec.2012.08.012 . .