TY  - CONF
AU  - Đukanović, Stefana
AU  - Lješević, Marija
AU  - Simin, Nataša
AU  - Lončarević, Branka
AU  - Ganić, Tea
AU  - Nikolić, Biljana
AU  - Cvetković, Stefana
AU  - Mitić-Ćulafić, Dragana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5148
AB  - The aim of the study was to chemically characterize the ethyl-acetate extract of F. alnus bark and investigate its antioxidant and antibacterial potential. Qualitative analysis of extract was done by GCxGC-MS while selected phenolics were quantified by LC-MS/MS analysis.
PB  - Opole, Poland : University of Opole
C3  - QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland
T1  - Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract
SP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_cer_5148
ER  - 

@conference{
author = "Đukanović, Stefana and Lješević, Marija and Simin, Nataša and Lončarević, Branka and Ganić, Tea and Nikolić, Biljana and Cvetković, Stefana and Mitić-Ćulafić, Dragana",
year = "2021",
abstract = "The aim of the study was to chemically characterize the ethyl-acetate extract of F. alnus bark and investigate its antioxidant and antibacterial potential. Qualitative analysis of extract was done by GCxGC-MS while selected phenolics were quantified by LC-MS/MS analysis.",
publisher = "Opole, Poland : University of Opole",
journal = "QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland",
title = "Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract",
pages = "147",
url = "https://hdl.handle.net/21.15107/rcub_cer_5148"
}

Đukanović, S., Lješević, M., Simin, N., Lončarević, B., Ganić, T., Nikolić, B., Cvetković, S.,& Mitić-Ćulafić, D.. (2021). Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract. in QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland
Opole, Poland : University of Opole., 147.
https://hdl.handle.net/21.15107/rcub_cer_5148

Đukanović S, Lješević M, Simin N, Lončarević B, Ganić T, Nikolić B, Cvetković S, Mitić-Ćulafić D. Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract. in QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland. 2021;:147.
https://hdl.handle.net/21.15107/rcub_cer_5148 .

Đukanović, Stefana, Lješević, Marija, Simin, Nataša, Lončarević, Branka, Ganić, Tea, Nikolić, Biljana, Cvetković, Stefana, Mitić-Ćulafić, Dragana, "Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract" in QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland (2021):147,
https://hdl.handle.net/21.15107/rcub_cer_5148 .

TY  - JOUR
AU  - Đukanović, Stefana
AU  - Cvetković, Stefana
AU  - Lončarević, Branka
AU  - Lješević, Marija
AU  - Nikolić, Biljana
AU  - Simin, Nataša
AU  - Bekvalac, Kristina
AU  - Kekić, Dušan
AU  - Mitić-Ćulafić, Dragana
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3946
AB  - Staphylococcus aureus is one of the leading causative of numerous infections that is resistant to various antibiotics. Since that we are facing with a serious problem of bacterial resistance it is necessary to find a new antibacterial agents in fight with it. Frangula alnus is traditionally used plant but its antibiofilm potential is poorly investigated. The aim of the study was to investigate antibiofilm activity of F. alnus ethyl-acetate extract (FA) toward S. aureus ATCC strains and clinical isolates as well as its influence on respiration in planktonic and biofilm form. The qualitative GCxGC–MS and quantitative LC–MS/MS analysis revealed that FA extract was rich in phenols and flavonoids and emodin, chatechin, and ester 4-ethoxy benzoic acid were the most dominant components. Results obtained through microdillution assay showed that FA possesses strong antibacterial activity. Furthermore, crystal violet staining of biofilm biomass demonstrated that extract had strong effect on biofilm formation of all tested strains while effect on preformed biofilms was less pronounced. The effect on biofilm was confirmed with scanning electron microscopy where the changes in biofilm structure were noticed. The activity of extract on the consumption of O2 and production of CO2 was monitored using the Micro-Oxymax respirometer. Interestingly, respiration of the most strains was decreased in planktonic form as well as in biofilms. Results obtained in this study are a good basis for further research in order to discover the mechanism of action of the FA extract on connection between biofilm and respiration.
PB  - Elsevier
T2  - Industrial Crops & Products
T1  - Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract
VL  - 158
SP  - 113013
DO  - 10.1016/j.indcrop.2020.113013
ER  - 

@article{
author = "Đukanović, Stefana and Cvetković, Stefana and Lončarević, Branka and Lješević, Marija and Nikolić, Biljana and Simin, Nataša and Bekvalac, Kristina and Kekić, Dušan and Mitić-Ćulafić, Dragana",
year = "2020",
abstract = "Staphylococcus aureus is one of the leading causative of numerous infections that is resistant to various antibiotics. Since that we are facing with a serious problem of bacterial resistance it is necessary to find a new antibacterial agents in fight with it. Frangula alnus is traditionally used plant but its antibiofilm potential is poorly investigated. The aim of the study was to investigate antibiofilm activity of F. alnus ethyl-acetate extract (FA) toward S. aureus ATCC strains and clinical isolates as well as its influence on respiration in planktonic and biofilm form. The qualitative GCxGC–MS and quantitative LC–MS/MS analysis revealed that FA extract was rich in phenols and flavonoids and emodin, chatechin, and ester 4-ethoxy benzoic acid were the most dominant components. Results obtained through microdillution assay showed that FA possesses strong antibacterial activity. Furthermore, crystal violet staining of biofilm biomass demonstrated that extract had strong effect on biofilm formation of all tested strains while effect on preformed biofilms was less pronounced. The effect on biofilm was confirmed with scanning electron microscopy where the changes in biofilm structure were noticed. The activity of extract on the consumption of O2 and production of CO2 was monitored using the Micro-Oxymax respirometer. Interestingly, respiration of the most strains was decreased in planktonic form as well as in biofilms. Results obtained in this study are a good basis for further research in order to discover the mechanism of action of the FA extract on connection between biofilm and respiration.",
publisher = "Elsevier",
journal = "Industrial Crops & Products",
title = "Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract",
volume = "158",
pages = "113013",
doi = "10.1016/j.indcrop.2020.113013"
}

Đukanović, S., Cvetković, S., Lončarević, B., Lješević, M., Nikolić, B., Simin, N., Bekvalac, K., Kekić, D.,& Mitić-Ćulafić, D.. (2020). Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract. in Industrial Crops & Products
Elsevier., 158, 113013.
https://doi.org/10.1016/j.indcrop.2020.113013

Đukanović S, Cvetković S, Lončarević B, Lješević M, Nikolić B, Simin N, Bekvalac K, Kekić D, Mitić-Ćulafić D. Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract. in Industrial Crops & Products. 2020;158:113013.
doi:10.1016/j.indcrop.2020.113013 .

Đukanović, Stefana, Cvetković, Stefana, Lončarević, Branka, Lješević, Marija, Nikolić, Biljana, Simin, Nataša, Bekvalac, Kristina, Kekić, Dušan, Mitić-Ćulafić, Dragana, "Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract" in Industrial Crops & Products, 158 (2020):113013,
https://doi.org/10.1016/j.indcrop.2020.113013 . .

TY  - CONF
AU  - Aćimović, Milica
AU  - Stanković, Jovana
AU  - Cvetković, Mirjana
AU  - Simin, Nataša
AU  - Beara, Ivana
AU  - Lesjak, Marija
AU  - Popović, Vera
PY  - 2020
UR  - uri:http://www.gea.ucg.ac.me
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3954
AB  - Most of the Origanum species are locally distributed within the Mediterranean region
where they grow in the mountainous areas on the islands. Due to this, the rate of endemism is high,
as in case of dittany of Crete (O. dictamnus). O. vulgare possesses the largest distribution area and can
be found throughout the Mediterranean region, however, the yield and quality of the essential oil is
controlled genetically and strongly affected by the environmental influences. Origanum essential oils
predominantly containing carvacrol, are generally of superior quality and highly valuable raw
material for food as well as in pharmaceutical industry. Essential oil of O. dictimus contains 70.8% of
carvacrol, while O. vulgare ssp. hirtum essential oil contains 78.5%. This study indicates the high
quality of investigated Origanum species from Greece, and indicates it to be highly valuable raw
material for food and in pharmaceutical industry.
PB  - Faculty of Architecture – University of Montenegro
PB  - Faculty of Philosophy - University of Montenegro
PB  - Biotecnical faculty - University of Montenegro
C3  - Book of Proceedings - GEA (Geo Eco-Eco Agro) International Conference
T1  - Chemical composition of Origanum dictamnus and Origanum vulgare ssp. hirtum from Greece
VL  - 1
SP  - 244
EP  - 249
UR  - https://hdl.handle.net/21.15107/rcub_cer_3954
ER  - 

@conference{
author = "Aćimović, Milica and Stanković, Jovana and Cvetković, Mirjana and Simin, Nataša and Beara, Ivana and Lesjak, Marija and Popović, Vera",
year = "2020",
abstract = "Most of the Origanum species are locally distributed within the Mediterranean region
where they grow in the mountainous areas on the islands. Due to this, the rate of endemism is high,
as in case of dittany of Crete (O. dictamnus). O. vulgare possesses the largest distribution area and can
be found throughout the Mediterranean region, however, the yield and quality of the essential oil is
controlled genetically and strongly affected by the environmental influences. Origanum essential oils
predominantly containing carvacrol, are generally of superior quality and highly valuable raw
material for food as well as in pharmaceutical industry. Essential oil of O. dictimus contains 70.8% of
carvacrol, while O. vulgare ssp. hirtum essential oil contains 78.5%. This study indicates the high
quality of investigated Origanum species from Greece, and indicates it to be highly valuable raw
material for food and in pharmaceutical industry.",
publisher = "Faculty of Architecture – University of Montenegro, Faculty of Philosophy - University of Montenegro, Biotecnical faculty - University of Montenegro",
journal = "Book of Proceedings - GEA (Geo Eco-Eco Agro) International Conference",
title = "Chemical composition of Origanum dictamnus and Origanum vulgare ssp. hirtum from Greece",
volume = "1",
pages = "244-249",
url = "https://hdl.handle.net/21.15107/rcub_cer_3954"
}

Aćimović, M., Stanković, J., Cvetković, M., Simin, N., Beara, I., Lesjak, M.,& Popović, V.. (2020). Chemical composition of Origanum dictamnus and Origanum vulgare ssp. hirtum from Greece. in Book of Proceedings - GEA (Geo Eco-Eco Agro) International Conference
Faculty of Architecture – University of Montenegro., 1, 244-249.
https://hdl.handle.net/21.15107/rcub_cer_3954

Aćimović M, Stanković J, Cvetković M, Simin N, Beara I, Lesjak M, Popović V. Chemical composition of Origanum dictamnus and Origanum vulgare ssp. hirtum from Greece. in Book of Proceedings - GEA (Geo Eco-Eco Agro) International Conference. 2020;1:244-249.
https://hdl.handle.net/21.15107/rcub_cer_3954 .

Aćimović, Milica, Stanković, Jovana, Cvetković, Mirjana, Simin, Nataša, Beara, Ivana, Lesjak, Marija, Popović, Vera, "Chemical composition of Origanum dictamnus and Origanum vulgare ssp. hirtum from Greece" in Book of Proceedings - GEA (Geo Eco-Eco Agro) International Conference, 1 (2020):244-249,
https://hdl.handle.net/21.15107/rcub_cer_3954 .