TY  - JOUR
AU  - Nikolić, Milan
AU  - Stanić, Dragana
AU  - Antonijevic, Nadežda
AU  - Niketić, Vesna
PY  - 2004
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4167
AB  - Objective: To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated). Study design: Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer. Results: The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin- lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels. Conclusion: To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the formation of Hb-Ch. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.
PB  - Elsevier
T2  - Clinical Biochemistry
T1  - Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?
VL  - 37
IS  - 1
SP  - 22
EP  - 26
DO  - 10.1016/j.clinbiochem.2003.10.002
ER  - 

@article{
author = "Nikolić, Milan and Stanić, Dragana and Antonijevic, Nadežda and Niketić, Vesna",
year = "2004",
abstract = "Objective: To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated). Study design: Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer. Results: The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin- lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels. Conclusion: To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the formation of Hb-Ch. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.",
publisher = "Elsevier",
journal = "Clinical Biochemistry",
title = "Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?",
volume = "37",
number = "1",
pages = "22-26",
doi = "10.1016/j.clinbiochem.2003.10.002"
}

Nikolić, M., Stanić, D., Antonijevic, N.,& Niketić, V.. (2004). Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?. in Clinical Biochemistry
Elsevier., 37(1), 22-26.
https://doi.org/10.1016/j.clinbiochem.2003.10.002

Nikolić M, Stanić D, Antonijevic N, Niketić V. Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?. in Clinical Biochemistry. 2004;37(1):22-26.
doi:10.1016/j.clinbiochem.2003.10.002 .

Nikolić, Milan, Stanić, Dragana, Antonijevic, Nadežda, Niketić, Vesna, "Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?" in Clinical Biochemistry, 37, no. 1 (2004):22-26,
https://doi.org/10.1016/j.clinbiochem.2003.10.002 . .