TY  - JOUR
AU  - Popović, Slađana
AU  - Nikolić, Nataša
AU  - Jovanović, Jelena
AU  - Predojević, Dragana
AU  - Trbojević, Ivana
AU  - Manić, Ljiljana
AU  - Subakov-Simić, Gordana
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3067
AB  - Due to life in extreme environments, cyanobacteria and algae from cave biofilms that form at the entrances or deep inside the cave around artificial lights are of increasing interest to many scientists. It is well-known that many phototrophic microorganisms are first to colonize exposed substrata and produce the organic matter on which other biofilm constituents relly. Many studies dealing with phototrophic microorganisms from biofilms focus on the diversity and community composition of cyanobacteria and algae, while quantitative assessments are rarely implemented. Biofilm sampling was conducted in Degurić and Vernjikica Cave located in Western and Eastern Serbia, respectively. Ecological parameters (temperature, relative humidity, light intensity) and distance from the entrance were measured. Additionally, chlorophyll content, as well as biofilm parameters (water content, organic and inorganic matter) were determined. The abundance of phototrophic microorganisms was assessed on microscope slides which contained 1 mg of biofilm that was dehydrated for a short period of time and homogenized prior to slide preparation, and then rehydratated. The biomass of recorded cyanobacterial and algal taxa was calculated by applying geometric approximations and standard mathematical formulas. In Degurić Cave, at the sampling site where the highest biomass was documented, the higher diversity, water content and chlorophyll values were also recorded, while in Vernjikica Cave a high content of organic matter was documented. According to the multivariate analyses performed, the biomass of simple trichal Cyanobacteria, Bacillariophyta, and Xanthophyta was positively correlated with the content of organic matter in biofilm and light intensity, while coccoid and heterocytous Cyanobacteria and Chlorophyta showed a positive correlation with water content in the biofilm, relative humidity and distance from the entrance. The total biomass was positively correlated with the chlorophyll content, organic matter and light intensity, and negatively with the distance from the entrance.
PB  - University of South Florida Libraries
T2  - International Journal of Speleology
T1  - Cyanobacterial and algal abundance and biomass in cave biofilms and relation to environmental and biofilm parameters
VL  - 48
IS  - 1
SP  - 49
EP  - 61
DO  - 10.5038/1827-806X.48.1.2224
ER  - 

@article{
author = "Popović, Slađana and Nikolić, Nataša and Jovanović, Jelena and Predojević, Dragana and Trbojević, Ivana and Manić, Ljiljana and Subakov-Simić, Gordana",
year = "2019",
abstract = "Due to life in extreme environments, cyanobacteria and algae from cave biofilms that form at the entrances or deep inside the cave around artificial lights are of increasing interest to many scientists. It is well-known that many phototrophic microorganisms are first to colonize exposed substrata and produce the organic matter on which other biofilm constituents relly. Many studies dealing with phototrophic microorganisms from biofilms focus on the diversity and community composition of cyanobacteria and algae, while quantitative assessments are rarely implemented. Biofilm sampling was conducted in Degurić and Vernjikica Cave located in Western and Eastern Serbia, respectively. Ecological parameters (temperature, relative humidity, light intensity) and distance from the entrance were measured. Additionally, chlorophyll content, as well as biofilm parameters (water content, organic and inorganic matter) were determined. The abundance of phototrophic microorganisms was assessed on microscope slides which contained 1 mg of biofilm that was dehydrated for a short period of time and homogenized prior to slide preparation, and then rehydratated. The biomass of recorded cyanobacterial and algal taxa was calculated by applying geometric approximations and standard mathematical formulas. In Degurić Cave, at the sampling site where the highest biomass was documented, the higher diversity, water content and chlorophyll values were also recorded, while in Vernjikica Cave a high content of organic matter was documented. According to the multivariate analyses performed, the biomass of simple trichal Cyanobacteria, Bacillariophyta, and Xanthophyta was positively correlated with the content of organic matter in biofilm and light intensity, while coccoid and heterocytous Cyanobacteria and Chlorophyta showed a positive correlation with water content in the biofilm, relative humidity and distance from the entrance. The total biomass was positively correlated with the chlorophyll content, organic matter and light intensity, and negatively with the distance from the entrance.",
publisher = "University of South Florida Libraries",
journal = "International Journal of Speleology",
title = "Cyanobacterial and algal abundance and biomass in cave biofilms and relation to environmental and biofilm parameters",
volume = "48",
number = "1",
pages = "49-61",
doi = "10.5038/1827-806X.48.1.2224"
}

Popović, S., Nikolić, N., Jovanović, J., Predojević, D., Trbojević, I., Manić, L.,& Subakov-Simić, G.. (2019). Cyanobacterial and algal abundance and biomass in cave biofilms and relation to environmental and biofilm parameters. in International Journal of Speleology
University of South Florida Libraries., 48(1), 49-61.
https://doi.org/10.5038/1827-806X.48.1.2224

Popović S, Nikolić N, Jovanović J, Predojević D, Trbojević I, Manić L, Subakov-Simić G. Cyanobacterial and algal abundance and biomass in cave biofilms and relation to environmental and biofilm parameters. in International Journal of Speleology. 2019;48(1):49-61.
doi:10.5038/1827-806X.48.1.2224 .

Popović, Slađana, Nikolić, Nataša, Jovanović, Jelena, Predojević, Dragana, Trbojević, Ivana, Manić, Ljiljana, Subakov-Simić, Gordana, "Cyanobacterial and algal abundance and biomass in cave biofilms and relation to environmental and biofilm parameters" in International Journal of Speleology, 48, no. 1 (2019):49-61,
https://doi.org/10.5038/1827-806X.48.1.2224 . .