TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragićević, Milan B.
AU  - Tešević, Vele
AU  - Stanković Jeremić, Jovana
AU  - Maksimović, Vuk
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena M.
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4241
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down-vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI
T2  - Plants
T1  - Transcriptome profiling of the potato exposed to french marigold essential oil with a special emphasis on leaf starch metabolism and defense against colorado potato beetle
VL  - 10
IS  - 1
SP  - 172
DO  - 10.3390/plants10010172
ER  - 

@article{
author = "Stupar, Sofija and Dragićević, Milan B. and Tešević, Vele and Stanković Jeremić, Jovana and Maksimović, Vuk and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena M.",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down-vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars’ metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI",
journal = "Plants",
title = "Transcriptome profiling of the potato exposed to french marigold essential oil with a special emphasis on leaf starch metabolism and defense against colorado potato beetle",
volume = "10",
number = "1",
pages = "172",
doi = "10.3390/plants10010172"
}

Stupar, S., Dragićević, M. B., Tešević, V., Stanković Jeremić, J., Maksimović, V., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J. M.. (2021). Transcriptome profiling of the potato exposed to french marigold essential oil with a special emphasis on leaf starch metabolism and defense against colorado potato beetle. in Plants
MDPI., 10(1), 172.
https://doi.org/10.3390/plants10010172

Stupar S, Dragićević MB, Tešević V, Stanković Jeremić J, Maksimović V, Ćosić T, Devrnja N, Tubić L, Cingel A, Vinterhalter B, Ninković S, Savić JM. Transcriptome profiling of the potato exposed to french marigold essential oil with a special emphasis on leaf starch metabolism and defense against colorado potato beetle. in Plants. 2021;10(1):172.
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragićević, Milan B., Tešević, Vele, Stanković Jeremić, Jovana, Maksimović, Vuk, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena M., "Transcriptome profiling of the potato exposed to french marigold essential oil with a special emphasis on leaf starch metabolism and defense against colorado potato beetle" in Plants, 10, no. 1 (2021):172,
https://doi.org/10.3390/plants10010172 . .

TY  - CONF
AU  - Stupar, Sofija
AU  - Tešević, Vele
AU  - Stanković, Jovana
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7514
AB  - When attacked, plants activate defense mechanisms including volatile emission. Besides havinga pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground partsof plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysisrevealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)as the most abundant compounds. In order to investigate the potential of marigold EO to inducethe defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesisrelated PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EOvolatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,and plants were then additionally injured by mechanically wounding leaves across the leaf surface.After 24 h of wounding, another two leaves from each plant were collected and expressionof the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression ofall genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plantsexposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar tothat detected in untreated controls. These findings support the efforts for environmentally-safepest control in crops by using natural compounds.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil
SP  - 58
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_cer_7514
ER  - 

@conference{
author = "Stupar, Sofija and Tešević, Vele and Stanković, Jovana and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2018",
abstract = "When attacked, plants activate defense mechanisms including volatile emission. Besides havinga pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground partsof plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysisrevealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)as the most abundant compounds. In order to investigate the potential of marigold EO to inducethe defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesisrelated PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EOvolatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,and plants were then additionally injured by mechanically wounding leaves across the leaf surface.After 24 h of wounding, another two leaves from each plant were collected and expressionof the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression ofall genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plantsexposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar tothat detected in untreated controls. These findings support the efforts for environmentally-safepest control in crops by using natural compounds.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_cer_7514"
}

Stupar, S., Tešević, V., Stanković, J., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2018). Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 58-58.
https://hdl.handle.net/21.15107/rcub_cer_7514

Stupar S, Tešević V, Stanković J, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;:58-58.
https://hdl.handle.net/21.15107/rcub_cer_7514 .

Stupar, Sofija, Tešević, Vele, Stanković, Jovana, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018):58-58,
https://hdl.handle.net/21.15107/rcub_cer_7514 .