TY  - JOUR
AU  - Prlainović, Nevena Ž.
AU  - Bezbradica, Dejan I.
AU  - Knežević-Jugović, Zorica
AU  - Stevanović, Sanja
AU  - Avramov Ivić, Milka
AU  - Uskoković, Petar S.
AU  - Mijin, Dušan
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1297
AB  - In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.
PB  - Korean Society of Industrial Engineering Chemistry
T2  - Journal of Industrial and Engineering Chemistry
T1  - Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes
VL  - 19
IS  - 1
SP  - 279
EP  - 285
DO  - 10.1016/j.jiec.2012.08.012
ER  - 

@article{
author = "Prlainović, Nevena Ž. and Bezbradica, Dejan I. and Knežević-Jugović, Zorica and Stevanović, Sanja and Avramov Ivić, Milka and Uskoković, Petar S. and Mijin, Dušan",
year = "2013",
abstract = "In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.",
publisher = "Korean Society of Industrial Engineering Chemistry",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes",
volume = "19",
number = "1",
pages = "279-285",
doi = "10.1016/j.jiec.2012.08.012"
}

Prlainović, N. Ž., Bezbradica, D. I., Knežević-Jugović, Z., Stevanović, S., Avramov Ivić, M., Uskoković, P. S.,& Mijin, D.. (2013). Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry
Korean Society of Industrial Engineering Chemistry., 19(1), 279-285.
https://doi.org/10.1016/j.jiec.2012.08.012

Prlainović NŽ, Bezbradica DI, Knežević-Jugović Z, Stevanović S, Avramov Ivić M, Uskoković PS, Mijin D. Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes. in Journal of Industrial and Engineering Chemistry. 2013;19(1):279-285.
doi:10.1016/j.jiec.2012.08.012 .

Prlainović, Nevena Ž., Bezbradica, Dejan I., Knežević-Jugović, Zorica, Stevanović, Sanja, Avramov Ivić, Milka, Uskoković, Petar S., Mijin, Dušan, "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes" in Journal of Industrial and Engineering Chemistry, 19, no. 1 (2013):279-285,
https://doi.org/10.1016/j.jiec.2012.08.012 . .

TY  - JOUR
AU  - Bezbradica, Dejan I.
AU  - Ćorović, Jasmina J.
AU  - Prodanović, Radivoje
AU  - Milosavić, Nenad B.
AU  - Knežević, Zorica D.
PY  - 2005
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2686
AB  - An approach is presented for the stable covalent immobilization of Upase from Candida rugosa on Eupergit® with a high retention of hydrolytic activity. It comprises covalent bonding via lipase carbohydrate moiety previously modified by periodate oxidation, allowing a reduction in the involvement of the enzyme functional groups that are probably important in the catalytic mechanism. The hydrolytic activities of the lipase immobilized on Eupergif1 by two conventional methods (via oxirane group and via glutaralde-hyde) and with periodate method were compared. Results of lipase assays suggest that periodate method is superior for lipase immobilization on Eupergit® among methods applied in this study with respect to both, yield of immobilization and hydrolytic activity of the immobilized enzyme.
AB  - U ovom radu je ispitana mogućnost primene metode za kovalentno vezivanje lipaze iz Candida rugosa za komercijalni polimerni nosač Eupergit® kojom se dobijaju stabilni i aktivni imobilisani enzimi. Vezivanje se odvija preko ugljenohidratne komponente enzima, koja je prethodno modifikovana oksidacijom pomoću perjodata, a ne preko proteinske komponente, koja je važna za katalitičku aktivnost enzima. Hidrolitička aktivnost na ovaj način imobilisane lipaze upoređena je sa aktivnostima lipaza koje su imobilisane pomoću dve konvencionalne metode (vezivanje preko epoksidnih grupa nosača i vezivanje za nosač modifikovan glutaraldehidom). Rezultati ovog istraživanja pokazuju da je perjodatna metoda pogodnija za imobilizaciju lipaze na Eupergit® sa oba ispitivana aspekta: prinosa imobilizacije i hidrolitičke aktivnosti imobilisanog enzima.
T2  - Acta periodica technologica
T1  - Covalent immobilization of lipase from Candida rugosa on Eupergit®
T1  - Kovalentna imobilizacija lipaze iz Candida rugosa za Eupergit®
IS  - 36
SP  - 179
EP  - 186
DO  - 10.2298/APT0536179B
ER  - 

@article{
author = "Bezbradica, Dejan I. and Ćorović, Jasmina J. and Prodanović, Radivoje and Milosavić, Nenad B. and Knežević, Zorica D.",
year = "2005",
abstract = "An approach is presented for the stable covalent immobilization of Upase from Candida rugosa on Eupergit® with a high retention of hydrolytic activity. It comprises covalent bonding via lipase carbohydrate moiety previously modified by periodate oxidation, allowing a reduction in the involvement of the enzyme functional groups that are probably important in the catalytic mechanism. The hydrolytic activities of the lipase immobilized on Eupergif1 by two conventional methods (via oxirane group and via glutaralde-hyde) and with periodate method were compared. Results of lipase assays suggest that periodate method is superior for lipase immobilization on Eupergit® among methods applied in this study with respect to both, yield of immobilization and hydrolytic activity of the immobilized enzyme., U ovom radu je ispitana mogućnost primene metode za kovalentno vezivanje lipaze iz Candida rugosa za komercijalni polimerni nosač Eupergit® kojom se dobijaju stabilni i aktivni imobilisani enzimi. Vezivanje se odvija preko ugljenohidratne komponente enzima, koja je prethodno modifikovana oksidacijom pomoću perjodata, a ne preko proteinske komponente, koja je važna za katalitičku aktivnost enzima. Hidrolitička aktivnost na ovaj način imobilisane lipaze upoređena je sa aktivnostima lipaza koje su imobilisane pomoću dve konvencionalne metode (vezivanje preko epoksidnih grupa nosača i vezivanje za nosač modifikovan glutaraldehidom). Rezultati ovog istraživanja pokazuju da je perjodatna metoda pogodnija za imobilizaciju lipaze na Eupergit® sa oba ispitivana aspekta: prinosa imobilizacije i hidrolitičke aktivnosti imobilisanog enzima.",
journal = "Acta periodica technologica",
title = "Covalent immobilization of lipase from Candida rugosa on Eupergit®, Kovalentna imobilizacija lipaze iz Candida rugosa za Eupergit®",
number = "36",
pages = "179-186",
doi = "10.2298/APT0536179B"
}

Bezbradica, D. I., Ćorović, J. J., Prodanović, R., Milosavić, N. B.,& Knežević, Z. D.. (2005). Covalent immobilization of lipase from Candida rugosa on Eupergit®. in Acta periodica technologica(36), 179-186.
https://doi.org/10.2298/APT0536179B

Bezbradica DI, Ćorović JJ, Prodanović R, Milosavić NB, Knežević ZD. Covalent immobilization of lipase from Candida rugosa on Eupergit®. in Acta periodica technologica. 2005;(36):179-186.
doi:10.2298/APT0536179B .

Bezbradica, Dejan I., Ćorović, Jasmina J., Prodanović, Radivoje, Milosavić, Nenad B., Knežević, Zorica D., "Covalent immobilization of lipase from Candida rugosa on Eupergit®" in Acta periodica technologica, no. 36 (2005):179-186,
https://doi.org/10.2298/APT0536179B . .