TY  - JOUR
AU  - Gospodinova, Z.
AU  - Antov, G.
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Krasteva, N.
AU  - Pavlov, D.
AU  - Valcheva-Kuzmanova, S.
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7225
AB  - Background and objective: Cotinus coggygria Scop. is a valuable medicinal  plant species with pronounced pharmacological potential due to its numerous biological  activities. The herb is characterized by a high content of polyphenols among which is fustin.  The anticancer activities of fustin, however, are extremely weakly studied. The aim of the  present study was to investigate the in vitro antiproliferative potential of fustin isolated from  the heartwood of C. coggygria against cell lines originating from two of the most common  cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26). Materials  and methods: Cell growth inhibitory properties of fustin were examined by MTT assay.  Subsequently, phase-contrast and fl uorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin  cell line. Results: The obtained results showed that fustin reduced the proliferation of  all studied cell lines. The highest cytostatic eff ect was registered towards breast cancer  MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was  least aff ected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer  MDA-MB-231 cells indicated decreased density of cell monolayer and some morphological  alterations, signifi cant attenuation in the number of viable cells, and diminished clonogenic  ability of cells after fustin exposure. Conclusion: It could be concluded that fustin isolated  from the heartwood of medicinal plant C. coggygria possesses marked antiproliferative  properties against breast cancer cell line MDA-MB-231 which will be a subject of our more  detailed future investigations.
PB  - Walter de Gruyter GmbH
T2  - Acta Medica Bulgarica
T1  - Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of                    Cotinus Coggygria                    Scop. Against Breast and Colon Cancer Cell Lines
VL  - 50
IS  - 1
SP  - 27
EP  - 33
DO  - 10.2478/amb-2023-0004
ER  - 

@article{
author = "Gospodinova, Z. and Antov, G. and Novaković, Miroslav and Tešević, Vele and Krasteva, N. and Pavlov, D. and Valcheva-Kuzmanova, S.",
year = "2023",
abstract = "Background and objective: Cotinus coggygria Scop. is a valuable medicinal  plant species with pronounced pharmacological potential due to its numerous biological  activities. The herb is characterized by a high content of polyphenols among which is fustin.  The anticancer activities of fustin, however, are extremely weakly studied. The aim of the  present study was to investigate the in vitro antiproliferative potential of fustin isolated from  the heartwood of C. coggygria against cell lines originating from two of the most common  cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26). Materials  and methods: Cell growth inhibitory properties of fustin were examined by MTT assay.  Subsequently, phase-contrast and fl uorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin  cell line. Results: The obtained results showed that fustin reduced the proliferation of  all studied cell lines. The highest cytostatic eff ect was registered towards breast cancer  MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was  least aff ected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer  MDA-MB-231 cells indicated decreased density of cell monolayer and some morphological  alterations, signifi cant attenuation in the number of viable cells, and diminished clonogenic  ability of cells after fustin exposure. Conclusion: It could be concluded that fustin isolated  from the heartwood of medicinal plant C. coggygria possesses marked antiproliferative  properties against breast cancer cell line MDA-MB-231 which will be a subject of our more  detailed future investigations.",
publisher = "Walter de Gruyter GmbH",
journal = "Acta Medica Bulgarica",
title = "Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of                    Cotinus Coggygria                    Scop. Against Breast and Colon Cancer Cell Lines",
volume = "50",
number = "1",
pages = "27-33",
doi = "10.2478/amb-2023-0004"
}

Gospodinova, Z., Antov, G., Novaković, M., Tešević, V., Krasteva, N., Pavlov, D.,& Valcheva-Kuzmanova, S.. (2023). Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of                    Cotinus Coggygria                    Scop. Against Breast and Colon Cancer Cell Lines. in Acta Medica Bulgarica
Walter de Gruyter GmbH., 50(1), 27-33.
https://doi.org/10.2478/amb-2023-0004

Gospodinova Z, Antov G, Novaković M, Tešević V, Krasteva N, Pavlov D, Valcheva-Kuzmanova S. Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of                    Cotinus Coggygria                    Scop. Against Breast and Colon Cancer Cell Lines. in Acta Medica Bulgarica. 2023;50(1):27-33.
doi:10.2478/amb-2023-0004 .

Gospodinova, Z., Antov, G., Novaković, Miroslav, Tešević, Vele, Krasteva, N., Pavlov, D., Valcheva-Kuzmanova, S., "Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of                    Cotinus Coggygria                    Scop. Against Breast and Colon Cancer Cell Lines" in Acta Medica Bulgarica, 50, no. 1 (2023):27-33,
https://doi.org/10.2478/amb-2023-0004 . .