TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Zarić, Božidarka
AU  - Nešković-Konstantinović, Zora
AU  - Spasić, Snežana
AU  - Jones, David
AU  - Radojčić, Marija
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3945
AB  - To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-κB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4+ lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4+ cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4+ cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.
PB  - Taylor & Francis
T2  - Redox Report
T1  - Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects
VL  - 13
IS  - 1
SP  - 17
EP  - 22
DO  - 10.1179/135100008X259088
ER  - 

@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Zarić, Božidarka and Nešković-Konstantinović, Zora and Spasić, Snežana and Jones, David and Radojčić, Marija",
year = "2013",
abstract = "To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-κB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4+ lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4+ cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4+ cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.",
publisher = "Taylor & Francis",
journal = "Redox Report",
title = "Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects",
volume = "13",
number = "1",
pages = "17-22",
doi = "10.1179/135100008X259088"
}

Adžić, M., Nićiforović, A., Zarić, B., Nešković-Konstantinović, Z., Spasić, S., Jones, D.,& Radojčić, M.. (2013). Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects. in Redox Report
Taylor & Francis., 13(1), 17-22.
https://doi.org/10.1179/135100008X259088

Adžić M, Nićiforović A, Zarić B, Nešković-Konstantinović Z, Spasić S, Jones D, Radojčić M. Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects. in Redox Report. 2013;13(1):17-22.
doi:10.1179/135100008X259088 .

Adžić, Miroslav, Nićiforović, Ana, Zarić, Božidarka, Nešković-Konstantinović, Zora, Spasić, Snežana, Jones, David, Radojčić, Marija, "Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects" in Redox Report, 13, no. 1 (2013):17-22,
https://doi.org/10.1179/135100008X259088 . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Vucić, Vesna
AU  - Nešković-Konstantinović, Zora
AU  - Spasić, Snežana
AU  - Jones, David
AU  - Radojčić, Marija
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3965
AB  - There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappaB and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappaB and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.
PB  - Maney Publishing
T2  - Redox Report
T1  - Systemic NF-kappaB activation in blood cells of breast cancer patients
VL  - 11
IS  - 1
SP  - 39
SP  - 44
DO  - 10.1179/135100006X101002
ER  - 

@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Vucić, Vesna and Nešković-Konstantinović, Zora and Spasić, Snežana and Jones, David and Radojčić, Marija and Spasić, Mihajlo",
year = "2006",
abstract = "There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappaB and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappaB and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.",
publisher = "Maney Publishing",
journal = "Redox Report",
title = "Systemic NF-kappaB activation in blood cells of breast cancer patients",
volume = "11",
number = "1",
pages = "39-44",
doi = "10.1179/135100006X101002"
}

Adžić, M., Nićiforović, A., Vucić, V., Nešković-Konstantinović, Z., Spasić, S., Jones, D., Radojčić, M.,& Spasić, M.. (2006). Systemic NF-kappaB activation in blood cells of breast cancer patients. in Redox Report
Maney Publishing., 11(1), 39.
https://doi.org/10.1179/135100006X101002

Adžić M, Nićiforović A, Vucić V, Nešković-Konstantinović Z, Spasić S, Jones D, Radojčić M, Spasić M. Systemic NF-kappaB activation in blood cells of breast cancer patients. in Redox Report. 2006;11(1):39.
doi:10.1179/135100006X101002 .

Adžić, Miroslav, Nićiforović, Ana, Vucić, Vesna, Nešković-Konstantinović, Zora, Spasić, Snežana, Jones, David, Radojčić, Marija, Spasić, Mihajlo, "Systemic NF-kappaB activation in blood cells of breast cancer patients" in Redox Report, 11, no. 1 (2006):39,
https://doi.org/10.1179/135100006X101002 . .