TY  - JOUR
AU  - Borozan, Sunčica
AU  - Zlatović, Mario
AU  - Stojanović, Srđan
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2007
AB  - We analyzed the potential influence of anion-pi interactions on the stability of complexes of proteins and halogen-containing non-natural amino acids. Anion-pi interactions are distance and orientation dependent and our ab initio calculations showed that their energy can be lower than -8 kcal mol(-1), while most of their interaction energies lie in the range from -1 to -4 kcal mol(-1). About 20 % of these interactions were found to be repulsive. We have observed that Tyr has the highest occurrence among the aromatic residues involved in anion-pi interactions, while His made the least contribution. Furthermore, our study showed that 67 % of total interactions in the dataset are multiple anion-pi interactions. Most of the amino acid residues involved in anion-pi interactions tend to be buried in the solvent-excluded environment. The majority of the anion-pi interacting residues are located in regions with helical secondary structure. Analysis of stabilization centers for these complexes showed that all of the six residues capable of anion-pi interactions are important in locating one or more of such centers. We found that anion-pi interacting residues are sometimes involved in simultaneous interactions with halogens as well. With all that in mind, we can conclude that the anion-pi interactions can show significant influence on molecular organization and on the structural stability of the complexes of proteins and halogen-containing non-natural amino acids. Their influence should not be neglected in supramolecular chemistry and crystal engineering fields as well.
PB  - Springer, New York
T2  - Journal of Biological Inorganic Chemistry
T1  - Anion-pi interactions in complexes of proteins and halogen-containing amino acids
VL  - 21
IS  - 3
SP  - 357
EP  - 368
DO  - 10.1007/s00775-016-1346-y
ER  - 

@article{
author = "Borozan, Sunčica and Zlatović, Mario and Stojanović, Srđan",
year = "2016",
abstract = "We analyzed the potential influence of anion-pi interactions on the stability of complexes of proteins and halogen-containing non-natural amino acids. Anion-pi interactions are distance and orientation dependent and our ab initio calculations showed that their energy can be lower than -8 kcal mol(-1), while most of their interaction energies lie in the range from -1 to -4 kcal mol(-1). About 20 % of these interactions were found to be repulsive. We have observed that Tyr has the highest occurrence among the aromatic residues involved in anion-pi interactions, while His made the least contribution. Furthermore, our study showed that 67 % of total interactions in the dataset are multiple anion-pi interactions. Most of the amino acid residues involved in anion-pi interactions tend to be buried in the solvent-excluded environment. The majority of the anion-pi interacting residues are located in regions with helical secondary structure. Analysis of stabilization centers for these complexes showed that all of the six residues capable of anion-pi interactions are important in locating one or more of such centers. We found that anion-pi interacting residues are sometimes involved in simultaneous interactions with halogens as well. With all that in mind, we can conclude that the anion-pi interactions can show significant influence on molecular organization and on the structural stability of the complexes of proteins and halogen-containing non-natural amino acids. Their influence should not be neglected in supramolecular chemistry and crystal engineering fields as well.",
publisher = "Springer, New York",
journal = "Journal of Biological Inorganic Chemistry",
title = "Anion-pi interactions in complexes of proteins and halogen-containing amino acids",
volume = "21",
number = "3",
pages = "357-368",
doi = "10.1007/s00775-016-1346-y"
}

Borozan, S., Zlatović, M.,& Stojanović, S.. (2016). Anion-pi interactions in complexes of proteins and halogen-containing amino acids. in Journal of Biological Inorganic Chemistry
Springer, New York., 21(3), 357-368.
https://doi.org/10.1007/s00775-016-1346-y

Borozan S, Zlatović M, Stojanović S. Anion-pi interactions in complexes of proteins and halogen-containing amino acids. in Journal of Biological Inorganic Chemistry. 2016;21(3):357-368.
doi:10.1007/s00775-016-1346-y .

Borozan, Sunčica, Zlatović, Mario, Stojanović, Srđan, "Anion-pi interactions in complexes of proteins and halogen-containing amino acids" in Journal of Biological Inorganic Chemistry, 21, no. 3 (2016):357-368,
https://doi.org/10.1007/s00775-016-1346-y . .

TY  - JOUR
AU  - Radakovic, M.
AU  - Davitkov, D.
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
AU  - Stevanović, Jevrosima
AU  - Krstic, V.
AU  - Stanimirovic, Z.
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1947
AB  - The aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2-), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH). In addition, relative distribution of lactate dehydrogenase (LDH1-LDH5) activity and the DNA-damaging effects of T. equi infection were evaluated. Compared to control horses, horses infected with T. equi had significantly higher SOD1 activities (P  LT  0.05) and PHb (P  LT  0.01), NO2- (P  LT  0.001), NOx (P  LT  0.05) and MDA concentrations (P  LT  0.001), and significantly-lower EH (P  LT  0.001), CAT (P  LT  0.01) and PON1 (P  LT  0.001) activities, and thiol group concentrations (P  LT  0.05). The comet assay demonstrated significantly increased DNA damage in T. equi infected cells compared to non-infected cells (P  LT  0.001). Infected horses had significantly increased LDH5 isoenzyme activities (P  LT  0.05). There was higher production of ROS/RNS in T. equi-infected horses, which resulted in changes in osmotic fragility, damage to lipids, proteins and DNA, haemolysis and hepatocellular damage. Oxidative stress in horses naturally infected with T equi could contribute to the pathogenesis of the infection.
PB  - Elsevier Sci Ltd, Oxford
T2  - Veterinary Journal
T1  - Oxidative stress and DNA damage in horses naturally infected with Theileria equi
VL  - 217
SP  - 112
EP  - 118
DO  - 10.1016/j.tvjl.2016.10.003
ER  - 

@article{
author = "Radakovic, M. and Davitkov, D. and Borozan, Sunčica and Stojanović, Srđan and Stevanović, Jevrosima and Krstic, V. and Stanimirovic, Z.",
year = "2016",
abstract = "The aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2-), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH). In addition, relative distribution of lactate dehydrogenase (LDH1-LDH5) activity and the DNA-damaging effects of T. equi infection were evaluated. Compared to control horses, horses infected with T. equi had significantly higher SOD1 activities (P  LT  0.05) and PHb (P  LT  0.01), NO2- (P  LT  0.001), NOx (P  LT  0.05) and MDA concentrations (P  LT  0.001), and significantly-lower EH (P  LT  0.001), CAT (P  LT  0.01) and PON1 (P  LT  0.001) activities, and thiol group concentrations (P  LT  0.05). The comet assay demonstrated significantly increased DNA damage in T. equi infected cells compared to non-infected cells (P  LT  0.001). Infected horses had significantly increased LDH5 isoenzyme activities (P  LT  0.05). There was higher production of ROS/RNS in T. equi-infected horses, which resulted in changes in osmotic fragility, damage to lipids, proteins and DNA, haemolysis and hepatocellular damage. Oxidative stress in horses naturally infected with T equi could contribute to the pathogenesis of the infection.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Veterinary Journal",
title = "Oxidative stress and DNA damage in horses naturally infected with Theileria equi",
volume = "217",
pages = "112-118",
doi = "10.1016/j.tvjl.2016.10.003"
}

Radakovic, M., Davitkov, D., Borozan, S., Stojanović, S., Stevanović, J., Krstic, V.,& Stanimirovic, Z.. (2016). Oxidative stress and DNA damage in horses naturally infected with Theileria equi. in Veterinary Journal
Elsevier Sci Ltd, Oxford., 217, 112-118.
https://doi.org/10.1016/j.tvjl.2016.10.003

Radakovic M, Davitkov D, Borozan S, Stojanović S, Stevanović J, Krstic V, Stanimirovic Z. Oxidative stress and DNA damage in horses naturally infected with Theileria equi. in Veterinary Journal. 2016;217:112-118.
doi:10.1016/j.tvjl.2016.10.003 .

Radakovic, M., Davitkov, D., Borozan, Sunčica, Stojanović, Srđan, Stevanović, Jevrosima, Krstic, V., Stanimirovic, Z., "Oxidative stress and DNA damage in horses naturally infected with Theileria equi" in Veterinary Journal, 217 (2016):112-118,
https://doi.org/10.1016/j.tvjl.2016.10.003 . .

TY  - JOUR
AU  - Radakovic, M.
AU  - Davitkov, D.
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
AU  - Stevanović, Jevrosima
AU  - Krstic, V.
AU  - Stanimirovic, Z.
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4383
AB  - The aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2-), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH). In addition, relative distribution of lactate dehydrogenase (LDH1-LDH5) activity and the DNA-damaging effects of T. equi infection were evaluated. Compared to control horses, horses infected with T. equi had significantly higher SOD1 activities (P  LT  0.05) and PHb (P  LT  0.01), NO2- (P  LT  0.001), NOx (P  LT  0.05) and MDA concentrations (P  LT  0.001), and significantly-lower EH (P  LT  0.001), CAT (P  LT  0.01) and PON1 (P  LT  0.001) activities, and thiol group concentrations (P  LT  0.05). The comet assay demonstrated significantly increased DNA damage in T. equi infected cells compared to non-infected cells (P  LT  0.001). Infected horses had significantly increased LDH5 isoenzyme activities (P  LT  0.05). There was higher production of ROS/RNS in T. equi-infected horses, which resulted in changes in osmotic fragility, damage to lipids, proteins and DNA, haemolysis and hepatocellular damage. Oxidative stress in horses naturally infected with T equi could contribute to the pathogenesis of the infection.
PB  - Elsevier
T2  - Veterinary Journal
T1  - Oxidative stress and DNA damage in horses naturally infected with Theileria equi
VL  - 217
SP  - 112
EP  - 118
DO  - 10.1016/j.tvjl.2016.10.003
ER  - 

@article{
author = "Radakovic, M. and Davitkov, D. and Borozan, Sunčica and Stojanović, Srđan and Stevanović, Jevrosima and Krstic, V. and Stanimirovic, Z.",
year = "2016",
abstract = "The aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2-), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH). In addition, relative distribution of lactate dehydrogenase (LDH1-LDH5) activity and the DNA-damaging effects of T. equi infection were evaluated. Compared to control horses, horses infected with T. equi had significantly higher SOD1 activities (P  LT  0.05) and PHb (P  LT  0.01), NO2- (P  LT  0.001), NOx (P  LT  0.05) and MDA concentrations (P  LT  0.001), and significantly-lower EH (P  LT  0.001), CAT (P  LT  0.01) and PON1 (P  LT  0.001) activities, and thiol group concentrations (P  LT  0.05). The comet assay demonstrated significantly increased DNA damage in T. equi infected cells compared to non-infected cells (P  LT  0.001). Infected horses had significantly increased LDH5 isoenzyme activities (P  LT  0.05). There was higher production of ROS/RNS in T. equi-infected horses, which resulted in changes in osmotic fragility, damage to lipids, proteins and DNA, haemolysis and hepatocellular damage. Oxidative stress in horses naturally infected with T equi could contribute to the pathogenesis of the infection.",
publisher = "Elsevier",
journal = "Veterinary Journal",
title = "Oxidative stress and DNA damage in horses naturally infected with Theileria equi",
volume = "217",
pages = "112-118",
doi = "10.1016/j.tvjl.2016.10.003"
}

Radakovic, M., Davitkov, D., Borozan, S., Stojanović, S., Stevanović, J., Krstic, V.,& Stanimirovic, Z.. (2016). Oxidative stress and DNA damage in horses naturally infected with Theileria equi. in Veterinary Journal
Elsevier., 217, 112-118.
https://doi.org/10.1016/j.tvjl.2016.10.003

Radakovic M, Davitkov D, Borozan S, Stojanović S, Stevanović J, Krstic V, Stanimirovic Z. Oxidative stress and DNA damage in horses naturally infected with Theileria equi. in Veterinary Journal. 2016;217:112-118.
doi:10.1016/j.tvjl.2016.10.003 .

Radakovic, M., Davitkov, D., Borozan, Sunčica, Stojanović, Srđan, Stevanović, Jevrosima, Krstic, V., Stanimirovic, Z., "Oxidative stress and DNA damage in horses naturally infected with Theileria equi" in Veterinary Journal, 217 (2016):112-118,
https://doi.org/10.1016/j.tvjl.2016.10.003 . .

TY  - JOUR
AU  - Zlatović, Mario
AU  - Borozan, Sunčica
AU  - Nikolić, Milan
AU  - Stojanović, Srđan
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1811
AB  - In this work, we have analyzed the influence of anion-pi interactions on the stability of high resolution protein-porphyrin complex crystal structures. The anion-pi interactions are distance and orientation dependent. Results of ab initio calculations of stabilization energies showed that they lie mostly in the range from -2 to -4 kcal mol(-1) with some of the anion-pi interactions having stabilization energies of up to -16 kcal mol(-1). In the anionic group, the numbers of anion-pi interactions involving Asp and Glu are similar, while His is more often involved in these interactions than other aromatic residues. Furthermore, our study showed that in the dataset used about 70% of the investigated anion-pi interactions are in fact multiple anion-pi interactions. Our results suggest that interacting residues are predominantly located in buried and partially buried regions. The secondary structure of the anion-pi interaction involving residues shows that most of the interacting residues preferred to be in helix conformations. Significant numbers of aromatic residues involved in anion-pi interactions have one or more stabilization centers, providing additional stability to the protein-porphyrin complexes. The conservation patterns indicate that more than half of the residues involved in these interactions are evolutionarily conserved, indicating that the contribution of the anion-pi interaction is an important factor for the structural stability of the investigated protein-porphyrin complexes.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Anion-pi interactions in protein-porphyrin complexes
VL  - 5
IS  - 48
SP  - 38361
EP  - 38372
DO  - 10.1039/c5ra03373j
ER  - 

@article{
author = "Zlatović, Mario and Borozan, Sunčica and Nikolić, Milan and Stojanović, Srđan",
year = "2015",
abstract = "In this work, we have analyzed the influence of anion-pi interactions on the stability of high resolution protein-porphyrin complex crystal structures. The anion-pi interactions are distance and orientation dependent. Results of ab initio calculations of stabilization energies showed that they lie mostly in the range from -2 to -4 kcal mol(-1) with some of the anion-pi interactions having stabilization energies of up to -16 kcal mol(-1). In the anionic group, the numbers of anion-pi interactions involving Asp and Glu are similar, while His is more often involved in these interactions than other aromatic residues. Furthermore, our study showed that in the dataset used about 70% of the investigated anion-pi interactions are in fact multiple anion-pi interactions. Our results suggest that interacting residues are predominantly located in buried and partially buried regions. The secondary structure of the anion-pi interaction involving residues shows that most of the interacting residues preferred to be in helix conformations. Significant numbers of aromatic residues involved in anion-pi interactions have one or more stabilization centers, providing additional stability to the protein-porphyrin complexes. The conservation patterns indicate that more than half of the residues involved in these interactions are evolutionarily conserved, indicating that the contribution of the anion-pi interaction is an important factor for the structural stability of the investigated protein-porphyrin complexes.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Anion-pi interactions in protein-porphyrin complexes",
volume = "5",
number = "48",
pages = "38361-38372",
doi = "10.1039/c5ra03373j"
}

Zlatović, M., Borozan, S., Nikolić, M.,& Stojanović, S.. (2015). Anion-pi interactions in protein-porphyrin complexes. in RSC Advances
Royal Soc Chemistry, Cambridge., 5(48), 38361-38372.
https://doi.org/10.1039/c5ra03373j

Zlatović M, Borozan S, Nikolić M, Stojanović S. Anion-pi interactions in protein-porphyrin complexes. in RSC Advances. 2015;5(48):38361-38372.
doi:10.1039/c5ra03373j .

Zlatović, Mario, Borozan, Sunčica, Nikolić, Milan, Stojanović, Srđan, "Anion-pi interactions in protein-porphyrin complexes" in RSC Advances, 5, no. 48 (2015):38361-38372,
https://doi.org/10.1039/c5ra03373j . .

TY  - JOUR
AU  - Cabarkapa, Andrea
AU  - Borozan, Sunčica
AU  - Živković, Lada
AU  - Milanovic-Cabarkapa, Mirjana
AU  - Stojanović, Srđan
AU  - Bajic, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1706
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Toxicological and Environmental Chemistry
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 

@article{
author = "Cabarkapa, Andrea and Borozan, Sunčica and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Stojanović, Srđan and Bajic, Vladan and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Toxicological and Environmental Chemistry",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}

Cabarkapa, A., Borozan, S., Živković, L., Milanovic-Cabarkapa, M., Stojanović, S., Bajic, V.,& Spremo-Potparević, B.. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicological and Environmental Chemistry
Taylor & Francis Ltd, Abingdon., 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973

Cabarkapa A, Borozan S, Živković L, Milanovic-Cabarkapa M, Stojanović S, Bajic V, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicological and Environmental Chemistry. 2015;97(6):799-813.
doi:10.1080/02772248.2015.1060973 .

Cabarkapa, Andrea, Borozan, Sunčica, Živković, Lada, Milanovic-Cabarkapa, Mirjana, Stojanović, Srđan, Bajic, Vladan, Spremo-Potparević, Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" in Toxicological and Environmental Chemistry, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 . .

TY  - JOUR
AU  - Cabarkapa, A
AU  - Borozan, Sunčica
AU  - Zivkovic, L
AU  - Stojanović, Srđan
AU  - Milanovic-Cabarkapa, M
AU  - Bajic, V
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1800
AB  - Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study
VL  - 242
SP  - 171
EP  - 178
DO  - 10.1016/j.cbi.2015.10.002
ER  - 

@article{
author = "Cabarkapa, A and Borozan, Sunčica and Zivkovic, L and Stojanović, Srđan and Milanovic-Cabarkapa, M and Bajic, V and Spremo-Potparević, Biljana",
year = "2015",
abstract = "Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study",
volume = "242",
pages = "171-178",
doi = "10.1016/j.cbi.2015.10.002"
}

Cabarkapa, A., Borozan, S., Zivkovic, L., Stojanović, S., Milanovic-Cabarkapa, M., Bajic, V.,& Spremo-Potparević, B.. (2015). CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 242, 171-178.
https://doi.org/10.1016/j.cbi.2015.10.002

Cabarkapa A, Borozan S, Zivkovic L, Stojanović S, Milanovic-Cabarkapa M, Bajic V, Spremo-Potparević B. CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions. 2015;242:171-178.
doi:10.1016/j.cbi.2015.10.002 .

Cabarkapa, A, Borozan, Sunčica, Zivkovic, L, Stojanović, Srđan, Milanovic-Cabarkapa, M, Bajic, V, Spremo-Potparević, Biljana, "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study" in Chemico-Biological Interactions, 242 (2015):171-178,
https://doi.org/10.1016/j.cbi.2015.10.002 . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Borozan, Sunčica
AU  - Živković, Lada
AU  - Milanović-Čabarkapa, Mirjana
AU  - Stojanović, Srđan
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2440
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/3428
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3121
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Toxicology
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 

@article{
author = "Čabarkapa, Andrea and Borozan, Sunčica and Živković, Lada and Milanović-Čabarkapa, Mirjana and Stojanović, Srđan and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Toxicology",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}

Čabarkapa, A., Borozan, S., Živković, L., Milanović-Čabarkapa, M., Stojanović, S., Bajić, V.,& Spremo-Potparević, B.. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicology
Taylor & Francis Ltd, Abingdon., 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973

Čabarkapa A, Borozan S, Živković L, Milanović-Čabarkapa M, Stojanović S, Bajić V, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicology. 2015;97(6):799-813.
doi:10.1080/02772248.2015.1060973 .

Čabarkapa, Andrea, Borozan, Sunčica, Živković, Lada, Milanović-Čabarkapa, Mirjana, Stojanović, Srđan, Bajić, Vladan, Spremo-Potparević, Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" in Toxicology, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 . .

TY  - CONF
AU  - Borozan, Sunčica
AU  - Bošnjak, Ivan
AU  - Katić-Radivojević, Sofija
AU  - Jović, Slavoljub
AU  - Krstić, Milena
AU  - Stojanović, Srđan
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6510
AB  - Infectious larvae of equine parasites: Strongylidae and
Ascaridae may induce direct as well as indirect damage, during their
migration within the host. The aim of this study was to evaluate changes in hematological
and biochemical parameters in naturally infected horses, with
the above mentioned parasites.
PB  - International Society for Infectious Diseases
C3  - International Meeting on Emerging Diseases and Surveillance, IMED, October 31- November 3, 2014, Vienna, Austria
T1  - Horses naturally infected with Strongylidae and Ascaridae: Evaluation of hematological and some biochemical parameters
SP  - 160
EP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_cer_6510
ER  - 

@conference{
author = "Borozan, Sunčica and Bošnjak, Ivan and Katić-Radivojević, Sofija and Jović, Slavoljub and Krstić, Milena and Stojanović, Srđan",
year = "2014",
abstract = "Infectious larvae of equine parasites: Strongylidae and
Ascaridae may induce direct as well as indirect damage, during their
migration within the host. The aim of this study was to evaluate changes in hematological
and biochemical parameters in naturally infected horses, with
the above mentioned parasites.",
publisher = "International Society for Infectious Diseases",
journal = "International Meeting on Emerging Diseases and Surveillance, IMED, October 31- November 3, 2014, Vienna, Austria",
title = "Horses naturally infected with Strongylidae and Ascaridae: Evaluation of hematological and some biochemical parameters",
pages = "160-160",
url = "https://hdl.handle.net/21.15107/rcub_cer_6510"
}

Borozan, S., Bošnjak, I., Katić-Radivojević, S., Jović, S., Krstić, M.,& Stojanović, S.. (2014). Horses naturally infected with Strongylidae and Ascaridae: Evaluation of hematological and some biochemical parameters. in International Meeting on Emerging Diseases and Surveillance, IMED, October 31- November 3, 2014, Vienna, Austria
International Society for Infectious Diseases., 160-160.
https://hdl.handle.net/21.15107/rcub_cer_6510

Borozan S, Bošnjak I, Katić-Radivojević S, Jović S, Krstić M, Stojanović S. Horses naturally infected with Strongylidae and Ascaridae: Evaluation of hematological and some biochemical parameters. in International Meeting on Emerging Diseases and Surveillance, IMED, October 31- November 3, 2014, Vienna, Austria. 2014;:160-160.
https://hdl.handle.net/21.15107/rcub_cer_6510 .

Borozan, Sunčica, Bošnjak, Ivan, Katić-Radivojević, Sofija, Jović, Slavoljub, Krstić, Milena, Stojanović, Srđan, "Horses naturally infected with Strongylidae and Ascaridae: Evaluation of hematological and some biochemical parameters" in International Meeting on Emerging Diseases and Surveillance, IMED, October 31- November 3, 2014, Vienna, Austria (2014):160-160,
https://hdl.handle.net/21.15107/rcub_cer_6510 .

TY  - JOUR
AU  - Dimitrijević, Blagoje P.
AU  - Borozan, Sunčica
AU  - Jović, Slavoljub
AU  - Bacić, Dragan
AU  - Katić-Radivojević, Sofija
AU  - Stojanović, Srđan
AU  - Savić, Mila
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1157
AB  - The aim of this report was to study the biochemical parameters in sheep blood under conditions of various intensities of parasitic infection with Strongyloides papillosus, as well as after therapy with albendazole (ABZ). Investigations were performed on sheep of the Würtemberg race (n = 30) in which were detected mild, moderate and high intensities of parasitic infection with S. papillosus. The control group (n = 10) was composed of sheep negative to parasitic infections. The degree and type of changes were monitored by determining the concentrations of glucose, total proteins, albumin, A/G ratio, AST, urea, total bilirubin, calcium, phosphorus, total LDH activity and isoenzymatic LDH1-5 distributions. On the basis of the obtained results, we determined, through isoenzymatic LDH distribution, that during parasitic infection with S. papillosus, there is ongoing damage to the liver, heart muscle and lung, while after therapy with ABZ, the liver suffers the most damage. The concentration of glucose, total proteins and albumin fell linearly with the rise in the intensity of parasitic infection (p LT 0.05), while after therapy with ABZ, the fall of these parameters was at a statistically significantly higher level (p LT 0.01). The activity of AST, concentration of urea and total bilirubin also rose linearly with the intensity of parasitic infection (p LT 0.05). After therapy with ABZ, the activity of AST and the concentration of total bilirubin were at a statistically significantly higher level (p LT 0.001), while the concentration of urea retained the same levels as in the case of parasitic infection. Values of calcium concentrations (p>0.05) and phosphorus (p LT 0.05) also fall linearly with the rise of the intesity of the parasitic infection. The trend in the concentration fall of these macroelements, continues also after treatment with albendazole (p LT 0.001). Having in mind our previous studies in the field of oxidative stress and phenomena lying behind these changes, we strongly recommend that in antiparasitic treatment protocols, beside antihelminthics, compounds with antioxidative properties should also be used.
AB  - Cilj ovog rada je bio da se ispitaju biohemijski parametri u krvi ovaca u uslovima različitog intenziteta parazitske infekcije sa Strongyloides papillosus, kao i nakon terapije sa albendazolom (ABZ). Istraživanje je izvedeno na ovcama Wurtemberg rase (n = 30) kod kojih je utvrđen blagi, srednji i visoki intenzitet parazitske infekcije sa S. papillosus. Kontrolnu grupu (n = 10) činile su ovce negativne na prisustvo parazitske infekcije. Stepen i vrsta promene praćeni su određivanjem koncentracije glukoze, ukupnih proteina, albumina, A/G ratio, AST, urea, ukupnog bilirubina, kalcijuma, fosfora, ukupne aktivnosti LDH i izoenzimske distribucije LDH1-5. Na osnovu dobijenih rezultata utvrdili smo, preko izoenzimske distribucije LDH, da u toku parazitske infekcije sa S. papillosus dolazi do oštećenja jetre, srčanog mišića i pluća, dok nakon terapije sa ABZ jetra je organ koji je najviše oštećen. Koncentracija glukoze, ukupnih proteina i albumina linearno je opadala sa rastom intenziteta parazitske infekcije (p LT 0.05), a nakon terapije sa ABZ pad koncentracije ovih parametara bio je na statistički značajnom većem nivou (p LT 0.01). Aktivnost AST, koncentracija uree i ukupnog bilirubina takođe su linearno rasle sa intenzitetom parazitske infekcije (p LT 0.05). Nakon terapije sa ABZ aktivnost AST i koncentracija ukupnog bilirubina bile su na statistički značajno većem nivou (p LT 0.001), dok je koncentracija uree zadržala iste nivoe kao u slučaju parazitske infekcije. Vrednosti koncentracija kalcijuma (p > 0.05) i fosfora (p LT 0.05) takođe linearno opadaju sa rastom intenziteta parazitske infekcije. Trend pada koncentracije ovih makroelemenata, nastavlja se i nakon terapije sa albendazolom (p LT 0.001). Imajući u vidu naša prethodna istraživanja na polju oksidativnog stresa, fenomena koji se nalazi u osnovi ovih promena, strogo preporučujemo da se u antiparazitske protokole, pored antihelmintika koriste i preparati sa antioksidativnim osobinama.
PB  - Veterinary Faculty, Univ Beogradu, Belgrade
T2  - Acta veterinaria
T1  - The effect of the intensity of parasitic infection with Strongyloides papillosus and albendazole therapy on biochemical parameters in sheep blood
T1  - Uticaj intenziteta parazitske infekcije sa Strongyloides papillosus i terapije albendazolom na biohemijske parametre u krvi ovaca
VL  - 63
IS  - 5-6
SP  - 581
EP  - 600
DO  - 10.2298/AVB1306581D
ER  - 

@article{
author = "Dimitrijević, Blagoje P. and Borozan, Sunčica and Jović, Slavoljub and Bacić, Dragan and Katić-Radivojević, Sofija and Stojanović, Srđan and Savić, Mila",
year = "2013",
abstract = "The aim of this report was to study the biochemical parameters in sheep blood under conditions of various intensities of parasitic infection with Strongyloides papillosus, as well as after therapy with albendazole (ABZ). Investigations were performed on sheep of the Würtemberg race (n = 30) in which were detected mild, moderate and high intensities of parasitic infection with S. papillosus. The control group (n = 10) was composed of sheep negative to parasitic infections. The degree and type of changes were monitored by determining the concentrations of glucose, total proteins, albumin, A/G ratio, AST, urea, total bilirubin, calcium, phosphorus, total LDH activity and isoenzymatic LDH1-5 distributions. On the basis of the obtained results, we determined, through isoenzymatic LDH distribution, that during parasitic infection with S. papillosus, there is ongoing damage to the liver, heart muscle and lung, while after therapy with ABZ, the liver suffers the most damage. The concentration of glucose, total proteins and albumin fell linearly with the rise in the intensity of parasitic infection (p LT 0.05), while after therapy with ABZ, the fall of these parameters was at a statistically significantly higher level (p LT 0.01). The activity of AST, concentration of urea and total bilirubin also rose linearly with the intensity of parasitic infection (p LT 0.05). After therapy with ABZ, the activity of AST and the concentration of total bilirubin were at a statistically significantly higher level (p LT 0.001), while the concentration of urea retained the same levels as in the case of parasitic infection. Values of calcium concentrations (p>0.05) and phosphorus (p LT 0.05) also fall linearly with the rise of the intesity of the parasitic infection. The trend in the concentration fall of these macroelements, continues also after treatment with albendazole (p LT 0.001). Having in mind our previous studies in the field of oxidative stress and phenomena lying behind these changes, we strongly recommend that in antiparasitic treatment protocols, beside antihelminthics, compounds with antioxidative properties should also be used., Cilj ovog rada je bio da se ispitaju biohemijski parametri u krvi ovaca u uslovima različitog intenziteta parazitske infekcije sa Strongyloides papillosus, kao i nakon terapije sa albendazolom (ABZ). Istraživanje je izvedeno na ovcama Wurtemberg rase (n = 30) kod kojih je utvrđen blagi, srednji i visoki intenzitet parazitske infekcije sa S. papillosus. Kontrolnu grupu (n = 10) činile su ovce negativne na prisustvo parazitske infekcije. Stepen i vrsta promene praćeni su određivanjem koncentracije glukoze, ukupnih proteina, albumina, A/G ratio, AST, urea, ukupnog bilirubina, kalcijuma, fosfora, ukupne aktivnosti LDH i izoenzimske distribucije LDH1-5. Na osnovu dobijenih rezultata utvrdili smo, preko izoenzimske distribucije LDH, da u toku parazitske infekcije sa S. papillosus dolazi do oštećenja jetre, srčanog mišića i pluća, dok nakon terapije sa ABZ jetra je organ koji je najviše oštećen. Koncentracija glukoze, ukupnih proteina i albumina linearno je opadala sa rastom intenziteta parazitske infekcije (p LT 0.05), a nakon terapije sa ABZ pad koncentracije ovih parametara bio je na statistički značajnom većem nivou (p LT 0.01). Aktivnost AST, koncentracija uree i ukupnog bilirubina takođe su linearno rasle sa intenzitetom parazitske infekcije (p LT 0.05). Nakon terapije sa ABZ aktivnost AST i koncentracija ukupnog bilirubina bile su na statistički značajno većem nivou (p LT 0.001), dok je koncentracija uree zadržala iste nivoe kao u slučaju parazitske infekcije. Vrednosti koncentracija kalcijuma (p > 0.05) i fosfora (p LT 0.05) takođe linearno opadaju sa rastom intenziteta parazitske infekcije. Trend pada koncentracije ovih makroelemenata, nastavlja se i nakon terapije sa albendazolom (p LT 0.001). Imajući u vidu naša prethodna istraživanja na polju oksidativnog stresa, fenomena koji se nalazi u osnovi ovih promena, strogo preporučujemo da se u antiparazitske protokole, pored antihelmintika koriste i preparati sa antioksidativnim osobinama.",
publisher = "Veterinary Faculty, Univ Beogradu, Belgrade",
journal = "Acta veterinaria",
title = "The effect of the intensity of parasitic infection with Strongyloides papillosus and albendazole therapy on biochemical parameters in sheep blood, Uticaj intenziteta parazitske infekcije sa Strongyloides papillosus i terapije albendazolom na biohemijske parametre u krvi ovaca",
volume = "63",
number = "5-6",
pages = "581-600",
doi = "10.2298/AVB1306581D"
}

Dimitrijević, B. P., Borozan, S., Jović, S., Bacić, D., Katić-Radivojević, S., Stojanović, S.,& Savić, M.. (2013). The effect of the intensity of parasitic infection with Strongyloides papillosus and albendazole therapy on biochemical parameters in sheep blood. in Acta veterinaria
Veterinary Faculty, Univ Beogradu, Belgrade., 63(5-6), 581-600.
https://doi.org/10.2298/AVB1306581D

Dimitrijević BP, Borozan S, Jović S, Bacić D, Katić-Radivojević S, Stojanović S, Savić M. The effect of the intensity of parasitic infection with Strongyloides papillosus and albendazole therapy on biochemical parameters in sheep blood. in Acta veterinaria. 2013;63(5-6):581-600.
doi:10.2298/AVB1306581D .

Dimitrijević, Blagoje P., Borozan, Sunčica, Jović, Slavoljub, Bacić, Dragan, Katić-Radivojević, Sofija, Stojanović, Srđan, Savić, Mila, "The effect of the intensity of parasitic infection with Strongyloides papillosus and albendazole therapy on biochemical parameters in sheep blood" in Acta veterinaria, 63, no. 5-6 (2013):581-600,
https://doi.org/10.2298/AVB1306581D . .

TY  - JOUR
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1235
AB  - In this work, we have analyzed the influence of halogen bonding to the stability of 44 complexes of proteins and non-natural amino acids. Fluorine- and chlorine-containing non-natural amino acids are more prevalent in the dataset, and an even larger number of contacts made by iodine-containing ligands are found. Only few halogen bonds with the hydroxyl oxygens and carboxylate side chains are found in the dataset. Halogen bonds with the nitrogen-containing side chains have higher occurrence than other acceptors. Backbone carbonyl oxygens and nitrogens are to a substantial extent involved in our dataset We have observed a small percentage of interactions involving water as hydrogen bond donors. Additionally, most of the interacting residues comprising the interfaces also show a great degree of conservation. There is a clear interaction hot spot at distances of 3.5-3.7 angstrom and Theta(1) angles of 100-120 degrees. There is also a cluster of contacts featuring short distances (2.6-2.9 angstrom) but only nearly optimal Theta(1) angles (140-160 degrees). 51.3% of stabilizing residues are involved in building halogen bonds with the non-natural amino acids. We discovered three types of structural motifs significantly over-represented: beta-turn-ir, beta-turn-il and niche-4r. The halogen-bonding statistics of the dataset do not show any preference for alpha-helices (36%), beta-sheets (36%), or turns/coils (28%) structures. Most of the amino acid residues that were involved in halogen bonds prefer to be in the solvent excluded environment (buried). Furthermore, we have shown that in amino acid-protein complexes halogen atoms can sometimes be involved in hydrogen bonding interactions with hydrogen bonding-donors. The results from this study might be used for the rational design of halogenated ligands as inhibitors and drugs, and in biomolecular engineering.
PB  - Elsevier Sci Ltd, Oxford
T2  - Computational Biology and Chemistry
T1  - Halogen bonding in complexes of proteins and non-natural amino acids
VL  - 47
SP  - 231
EP  - 239
DO  - 10.1016/j.compbiolchem.2013.10.002
ER  - 

@article{
author = "Borozan, Sunčica and Stojanović, Srđan",
year = "2013",
abstract = "In this work, we have analyzed the influence of halogen bonding to the stability of 44 complexes of proteins and non-natural amino acids. Fluorine- and chlorine-containing non-natural amino acids are more prevalent in the dataset, and an even larger number of contacts made by iodine-containing ligands are found. Only few halogen bonds with the hydroxyl oxygens and carboxylate side chains are found in the dataset. Halogen bonds with the nitrogen-containing side chains have higher occurrence than other acceptors. Backbone carbonyl oxygens and nitrogens are to a substantial extent involved in our dataset We have observed a small percentage of interactions involving water as hydrogen bond donors. Additionally, most of the interacting residues comprising the interfaces also show a great degree of conservation. There is a clear interaction hot spot at distances of 3.5-3.7 angstrom and Theta(1) angles of 100-120 degrees. There is also a cluster of contacts featuring short distances (2.6-2.9 angstrom) but only nearly optimal Theta(1) angles (140-160 degrees). 51.3% of stabilizing residues are involved in building halogen bonds with the non-natural amino acids. We discovered three types of structural motifs significantly over-represented: beta-turn-ir, beta-turn-il and niche-4r. The halogen-bonding statistics of the dataset do not show any preference for alpha-helices (36%), beta-sheets (36%), or turns/coils (28%) structures. Most of the amino acid residues that were involved in halogen bonds prefer to be in the solvent excluded environment (buried). Furthermore, we have shown that in amino acid-protein complexes halogen atoms can sometimes be involved in hydrogen bonding interactions with hydrogen bonding-donors. The results from this study might be used for the rational design of halogenated ligands as inhibitors and drugs, and in biomolecular engineering.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Computational Biology and Chemistry",
title = "Halogen bonding in complexes of proteins and non-natural amino acids",
volume = "47",
pages = "231-239",
doi = "10.1016/j.compbiolchem.2013.10.002"
}

Borozan, S.,& Stojanović, S.. (2013). Halogen bonding in complexes of proteins and non-natural amino acids. in Computational Biology and Chemistry
Elsevier Sci Ltd, Oxford., 47, 231-239.
https://doi.org/10.1016/j.compbiolchem.2013.10.002

Borozan S, Stojanović S. Halogen bonding in complexes of proteins and non-natural amino acids. in Computational Biology and Chemistry. 2013;47:231-239.
doi:10.1016/j.compbiolchem.2013.10.002 .

Borozan, Sunčica, Stojanović, Srđan, "Halogen bonding in complexes of proteins and non-natural amino acids" in Computational Biology and Chemistry, 47 (2013):231-239,
https://doi.org/10.1016/j.compbiolchem.2013.10.002 . .

TY  - JOUR
AU  - Borozan, Sunčica
AU  - Dimitrijević, Blagoje P.
AU  - Stojanović, Srđan
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1247
AB  - In this work, we have analyzed the influence of cation-pi interactions to the stability of 59 high resolution protein-RNA complex crystal structures. The total number of Lys and Arg are similar in the dataset as well as the number of their interactions. On the other hand, the aromatic chains of purines are exhibiting more cation-pi interactions than pyrimidines. 35% of the total interactions in the dataset are involved in the formation of multiple cation-pi interactions. The multiple cation-pi interactions have been conserved more than the single interactions. The analysis of the geometry of the cation-pi interactions has revealed that the average distance (d) value falls into distinct ranges corresponding to the multiple (4.28 angstrom) and single (5.50 angstrom) cation-pi interactions. The G-Arg pair has the strongest interaction energy of -3.68 kcal mol(-1) among all the possible pairs of amino acids and bases. Further, we found that the cation-pi interactions due to five-membered rings of A and G are stronger than that with the atoms in six-membered rings. 8.7% stabilizing residues are involved in building cation-pi interactions with the nucleic bases. There are three types of structural motifs significantly over-represented in protein-RNA interfaces: beta-turn-ir, niche-4r and st-staple. Tetraloops and kink-turns are the most abundant RNA motifs in protein-RNA interfaces. Amino acids deployed in the protein-RNA interfaces are deposited in helices, sheets and coils. Arg and Lys, involved in cation-pi interactions, prefer to be in the solvent exposed surface. The results from this study might be used for structure-based prediction and as scaffolds for future protein-RNA complex design.
PB  - Elsevier Sci Ltd, Oxford
T2  - Computational Biology and Chemistry
T1  - Cation-pi interactions in high resolution protein-RNA complex crystal structures
VL  - 47
SP  - 105
EP  - 112
DO  - 10.1016/j.compbiolchem.2013.08.005
ER  - 

@article{
author = "Borozan, Sunčica and Dimitrijević, Blagoje P. and Stojanović, Srđan",
year = "2013",
abstract = "In this work, we have analyzed the influence of cation-pi interactions to the stability of 59 high resolution protein-RNA complex crystal structures. The total number of Lys and Arg are similar in the dataset as well as the number of their interactions. On the other hand, the aromatic chains of purines are exhibiting more cation-pi interactions than pyrimidines. 35% of the total interactions in the dataset are involved in the formation of multiple cation-pi interactions. The multiple cation-pi interactions have been conserved more than the single interactions. The analysis of the geometry of the cation-pi interactions has revealed that the average distance (d) value falls into distinct ranges corresponding to the multiple (4.28 angstrom) and single (5.50 angstrom) cation-pi interactions. The G-Arg pair has the strongest interaction energy of -3.68 kcal mol(-1) among all the possible pairs of amino acids and bases. Further, we found that the cation-pi interactions due to five-membered rings of A and G are stronger than that with the atoms in six-membered rings. 8.7% stabilizing residues are involved in building cation-pi interactions with the nucleic bases. There are three types of structural motifs significantly over-represented in protein-RNA interfaces: beta-turn-ir, niche-4r and st-staple. Tetraloops and kink-turns are the most abundant RNA motifs in protein-RNA interfaces. Amino acids deployed in the protein-RNA interfaces are deposited in helices, sheets and coils. Arg and Lys, involved in cation-pi interactions, prefer to be in the solvent exposed surface. The results from this study might be used for structure-based prediction and as scaffolds for future protein-RNA complex design.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Computational Biology and Chemistry",
title = "Cation-pi interactions in high resolution protein-RNA complex crystal structures",
volume = "47",
pages = "105-112",
doi = "10.1016/j.compbiolchem.2013.08.005"
}

Borozan, S., Dimitrijević, B. P.,& Stojanović, S.. (2013). Cation-pi interactions in high resolution protein-RNA complex crystal structures. in Computational Biology and Chemistry
Elsevier Sci Ltd, Oxford., 47, 105-112.
https://doi.org/10.1016/j.compbiolchem.2013.08.005

Borozan S, Dimitrijević BP, Stojanović S. Cation-pi interactions in high resolution protein-RNA complex crystal structures. in Computational Biology and Chemistry. 2013;47:105-112.
doi:10.1016/j.compbiolchem.2013.08.005 .

Borozan, Sunčica, Dimitrijević, Blagoje P., Stojanović, Srđan, "Cation-pi interactions in high resolution protein-RNA complex crystal structures" in Computational Biology and Chemistry, 47 (2013):105-112,
https://doi.org/10.1016/j.compbiolchem.2013.08.005 . .

TY  - JOUR
AU  - Dimitrijević, Blagoje P.
AU  - Borozan, Sunčica
AU  - Katic-Radivojevic, Sofija
AU  - Stojanović, Srđan
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/944
AB  - The objective of this study was to estimate and evaluate oxidative/nitrosative stress parameters in sheep infected with Strongyloides papillosus and after antihelminthic treatment with albendazole (ABZ). This parasite, especially during development stages can seriously damage parenchaematous organs during migration within the host. The presence of parasites leads to increased productions of reactive oxygen species (ROS) and reactive nitrogen species (RNS). It is also well known that certain drugs can be very harmful for the delicate oxidant-antioxidant equilibrium, provoking oxidative stress during their biotransformation. ABZ is a broad spectrum antihelminthic drug, frequently used in veterinary medicine for therapy of parasitic infections. The current research was performed on female Wurttemberg sheep (n = 48). The distribution of parasites in sheep was evaluated using the native smear coprological technique, by sedimentation and flotation methods, revealing the presence of S. papillosus. The degree of infection intensity per sheep was quantitatively established by the method of McMaster, the animals having been divided into three groups according to the intensity of infection; mild, moderate and high. The control group consisted of sheep negative to the parasites. After determining the type of parasite infection, the sheep were treated with ABZ, per orally, in single doses of 5 mg/kg per body weight. Sampling of feces for parasitological and blood for biochemical assaying was performed on the 0 and 21st day after treatment with ABZ. The oxidative stress parameters were measured for catalase activity (CAT), the red cell membrane damage by level of malondialdehyde (MDA), while carbonyl and thiol plasma protein group concentrations were used as indicators of the degree of protein oxidative modification. The activity of Cu,Zn-superoxide dismutase (SOD) and relative distribution of lactate dehydrogenase (LDH1-LDH5) activity were determined electrophoretically. The distribution of LDH isoenzymes in sheep moderately and highly infected with S. papillosus revealed that the parasite induced damage to the myocardial (LDH2), lung (LDH3) and liver cells (LDH5) in infected animals, while ABZ treatment only damaged liver cells (LDH5). The MDA concentration revealed that lipid peroxidation increased both in the presence of parasites and the antihelminthic formulation tested (p  LT  0.001) when compared to the control sheep, while the increase of carbonyl concentration (p  LT  0.001), as well as the observed decrease of thiol concentration (p  LT  0.001) indicated significant oxidative damage of plasma proteins in experimental sheep, when compared to the control animals. Our results indicate that S. papillosus induces oxidative/nitrosative stress in sheep. The antihelminthic treatment with ABZ further promotes the disbalance of oxidative-antioxidative equilibrium in all tested sheep.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep
VL  - 186
IS  - 3-4
SP  - 364
EP  - 375
DO  - 10.1016/j.vetpar.2011.11.017
ER  - 

@article{
author = "Dimitrijević, Blagoje P. and Borozan, Sunčica and Katic-Radivojevic, Sofija and Stojanović, Srđan",
year = "2012",
abstract = "The objective of this study was to estimate and evaluate oxidative/nitrosative stress parameters in sheep infected with Strongyloides papillosus and after antihelminthic treatment with albendazole (ABZ). This parasite, especially during development stages can seriously damage parenchaematous organs during migration within the host. The presence of parasites leads to increased productions of reactive oxygen species (ROS) and reactive nitrogen species (RNS). It is also well known that certain drugs can be very harmful for the delicate oxidant-antioxidant equilibrium, provoking oxidative stress during their biotransformation. ABZ is a broad spectrum antihelminthic drug, frequently used in veterinary medicine for therapy of parasitic infections. The current research was performed on female Wurttemberg sheep (n = 48). The distribution of parasites in sheep was evaluated using the native smear coprological technique, by sedimentation and flotation methods, revealing the presence of S. papillosus. The degree of infection intensity per sheep was quantitatively established by the method of McMaster, the animals having been divided into three groups according to the intensity of infection; mild, moderate and high. The control group consisted of sheep negative to the parasites. After determining the type of parasite infection, the sheep were treated with ABZ, per orally, in single doses of 5 mg/kg per body weight. Sampling of feces for parasitological and blood for biochemical assaying was performed on the 0 and 21st day after treatment with ABZ. The oxidative stress parameters were measured for catalase activity (CAT), the red cell membrane damage by level of malondialdehyde (MDA), while carbonyl and thiol plasma protein group concentrations were used as indicators of the degree of protein oxidative modification. The activity of Cu,Zn-superoxide dismutase (SOD) and relative distribution of lactate dehydrogenase (LDH1-LDH5) activity were determined electrophoretically. The distribution of LDH isoenzymes in sheep moderately and highly infected with S. papillosus revealed that the parasite induced damage to the myocardial (LDH2), lung (LDH3) and liver cells (LDH5) in infected animals, while ABZ treatment only damaged liver cells (LDH5). The MDA concentration revealed that lipid peroxidation increased both in the presence of parasites and the antihelminthic formulation tested (p  LT  0.001) when compared to the control sheep, while the increase of carbonyl concentration (p  LT  0.001), as well as the observed decrease of thiol concentration (p  LT  0.001) indicated significant oxidative damage of plasma proteins in experimental sheep, when compared to the control animals. Our results indicate that S. papillosus induces oxidative/nitrosative stress in sheep. The antihelminthic treatment with ABZ further promotes the disbalance of oxidative-antioxidative equilibrium in all tested sheep.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep",
volume = "186",
number = "3-4",
pages = "364-375",
doi = "10.1016/j.vetpar.2011.11.017"
}

Dimitrijević, B. P., Borozan, S., Katic-Radivojevic, S.,& Stojanović, S.. (2012). Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep. in Veterinary Parasitology
Elsevier., 186(3-4), 364-375.
https://doi.org/10.1016/j.vetpar.2011.11.017

Dimitrijević BP, Borozan S, Katic-Radivojevic S, Stojanović S. Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep. in Veterinary Parasitology. 2012;186(3-4):364-375.
doi:10.1016/j.vetpar.2011.11.017 .

Dimitrijević, Blagoje P., Borozan, Sunčica, Katic-Radivojevic, Sofija, Stojanović, Srđan, "Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep" in Veterinary Parasitology, 186, no. 3-4 (2012):364-375,
https://doi.org/10.1016/j.vetpar.2011.11.017 . .

TY  - JOUR
AU  - Dimitrijević, Blagoje P.
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1119
AB  - In this study we have described the pi-pi and cation-pi interactions between the porphyrin ring and the protein part of porphyrin-containing proteins to better understand their stabilizing role. The number of pi-pi interactions was higher than that of cation-pi interactions in the same set of proteins studied. The pyrrole groups of one porphyrin can be involved in pi-pi interactions with pi systems of another porphyrin in the protein. We have found 5.1% cation-pi interactions between porphyrin Fe2+ metal cations and pi systems of surrounding amino acids as well as the pyrrole rings of other porphyrins. We observed that most of the pi-pi interactions have an energy in the range -0.5 to -2.0 kcal mol(-1), while the cation-pi interactions showed an energy in the range 22 to -4 kcal mol(-1). Further, an appreciable number of metal/cation-pi interaction pairs have an energy in the range -6 to -13 kcal mol(-1). The preferred parallel-stacked orientation is found to be more stable than a T-shaped structure for the full set of pi-pi interaction pairs. In the case of cation-pi interactions, it was found that 44% of the cation-pi interactions involved planar stacking, 37% of the interactions belonged to the oblique category, and the remaining 19% of the interactions were of the orthogonal type. The separation distance between the cation group and the aromatic ring decreases as the interplanar angle decreases. Furthermore, in the present study we have found that 10.4% of pi residues and 3.9% of cationic residues were found to have one or more stabilization centers. Amino acids deployed in the environment of porphyrin rings are deposited in helices and coils. The results from this study might be used for structure-based porphyrin protein prediction and as scaffolds for future porphyrin-containing protein design.
PB  - The Royal Society of Chemistry
T2  - RSC Advances
T1  - pi-pi and cation-pi interactions in protein-porphyrin complex crystal structures
VL  - 2
IS  - 33
SP  - 12963
EP  - 12972
DO  - 10.1039/c2ra21937a
ER  - 

@article{
author = "Dimitrijević, Blagoje P. and Borozan, Sunčica and Stojanović, Srđan",
year = "2012",
abstract = "In this study we have described the pi-pi and cation-pi interactions between the porphyrin ring and the protein part of porphyrin-containing proteins to better understand their stabilizing role. The number of pi-pi interactions was higher than that of cation-pi interactions in the same set of proteins studied. The pyrrole groups of one porphyrin can be involved in pi-pi interactions with pi systems of another porphyrin in the protein. We have found 5.1% cation-pi interactions between porphyrin Fe2+ metal cations and pi systems of surrounding amino acids as well as the pyrrole rings of other porphyrins. We observed that most of the pi-pi interactions have an energy in the range -0.5 to -2.0 kcal mol(-1), while the cation-pi interactions showed an energy in the range 22 to -4 kcal mol(-1). Further, an appreciable number of metal/cation-pi interaction pairs have an energy in the range -6 to -13 kcal mol(-1). The preferred parallel-stacked orientation is found to be more stable than a T-shaped structure for the full set of pi-pi interaction pairs. In the case of cation-pi interactions, it was found that 44% of the cation-pi interactions involved planar stacking, 37% of the interactions belonged to the oblique category, and the remaining 19% of the interactions were of the orthogonal type. The separation distance between the cation group and the aromatic ring decreases as the interplanar angle decreases. Furthermore, in the present study we have found that 10.4% of pi residues and 3.9% of cationic residues were found to have one or more stabilization centers. Amino acids deployed in the environment of porphyrin rings are deposited in helices and coils. The results from this study might be used for structure-based porphyrin protein prediction and as scaffolds for future porphyrin-containing protein design.",
publisher = "The Royal Society of Chemistry",
journal = "RSC Advances",
title = "pi-pi and cation-pi interactions in protein-porphyrin complex crystal structures",
volume = "2",
number = "33",
pages = "12963-12972",
doi = "10.1039/c2ra21937a"
}

Dimitrijević, B. P., Borozan, S.,& Stojanović, S.. (2012). pi-pi and cation-pi interactions in protein-porphyrin complex crystal structures. in RSC Advances
The Royal Society of Chemistry., 2(33), 12963-12972.
https://doi.org/10.1039/c2ra21937a

Dimitrijević BP, Borozan S, Stojanović S. pi-pi and cation-pi interactions in protein-porphyrin complex crystal structures. in RSC Advances. 2012;2(33):12963-12972.
doi:10.1039/c2ra21937a .

Dimitrijević, Blagoje P., Borozan, Sunčica, Stojanović, Srđan, "pi-pi and cation-pi interactions in protein-porphyrin complex crystal structures" in RSC Advances, 2, no. 33 (2012):12963-12972,
https://doi.org/10.1039/c2ra21937a . .

TY  - JOUR
AU  - Gojgić-Cvijović, Gordana
AU  - Karadžić, Ivanka
AU  - Gopčević, Kristina
AU  - Borozan, Sunčica
PY  - 2006
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/228
AB  - Streptomycetes are common soil bacteria that grow in a filamentous form and produced spores. Secondary metabolites, particularly antibiotics, as well as extracellular enzymes produced by them have very significant role in the process of differentiation. The Slreptomyces hygroscopicus CH-7 strain produces polyketide antibiotics and an extracellular proteolytic complex. The aim of this work was to establish the influence of transcription and translation inhibitors on biosynthesis of polyene antibiotic and proteolytic enzymes. The inhibitors were added at inoculation and at specific time intervals of fermentation. The production of hexaene H-85 is susceptible to rifamycin (transcription inhibitor) and chloramphenicol (translation inhibitor). Rifamycin reduces the production and trypsin like protease (TLP) and aminopeptidase (AP). while chloramphenicol acts only on TLP. Simultaneously these inhibitors almost have no effect on strain growth.
AB  - Streptomicete su gram pozitivne bakterije čije je najvažnije stanište zemlja. Pri rastu na čvrstim podlogama i u submerznim uslovima grade supstratni i vazdušni micelijum. Morfološka diferencijacija je praćena produkcijom sekundarnih metabolita kao što su antibiotici, a i različitih hidrolitičkih enzima. Za mikroorganizme koji ih proizvode antibiotici predstavljaju kompetitivno oružje, agense za transport metala kao i efektore diferencijacije. Proteolitički enzimi, takođe, imaju određenu ulogu u razvojnom ciklusu aktinomiceta. Na primer, uključeni su u procese građenja vazdušnog micelija i sporulaciju. Soj Streptomyces hygroscopicus CH-7 pri fermentaciji proizvodi antibiotike poliketidne strukture i smesu ekstracelularnih proteolitičkih enzima. U ovom radu je ispitivan uticaj antibiotika rifamicina i hloramfenikola, inhibitora transkripcije i translacije, na produkciju polienskog antibiotika heksaena H-85 i proteolitičkih enzima. Inhibited su dodavani pri inokulaciji (O h) i u različitim vremenskim intervalima fermentacije (12-48 h). Dejstvom antibiotika inhibitora transkripcije i translacije utvrdjeno je da se enzim polien sintetaza transkribuje do 12. časa, a translacija teče do 48. časa fermentacije. Dodatak rifamicina pri zasejavanju drastično snižava proteolitičku aktivnost, a u kasnijim fazama izaziva smanjenje (36-80%). Hloramfenikol smanjuje produkciju tripsinu slične proteaze. ali nema dejstvo na aminopeptidaznu aktivnost. Rifamicin i hloramfenikol ne pokazuju značajno inhibitorno dejstvo na rast soja.
T2  - Acta biologica iugoslavica - serija B: Mikrobiologija
T1  - The effect of rifamycin and chloramphenicol on antibiotic and protease production by Streptomyces hygroscopicus CH-7
T1  - Dejstvo rifamicina i hloramfenikola na produkciju antibiotika i proteaza soja Streptomyces hygroscopicus CH-7
VL  - 43
IS  - 1
SP  - 31
EP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_cer_228
ER  - 

@article{
author = "Gojgić-Cvijović, Gordana and Karadžić, Ivanka and Gopčević, Kristina and Borozan, Sunčica",
year = "2006",
abstract = "Streptomycetes are common soil bacteria that grow in a filamentous form and produced spores. Secondary metabolites, particularly antibiotics, as well as extracellular enzymes produced by them have very significant role in the process of differentiation. The Slreptomyces hygroscopicus CH-7 strain produces polyketide antibiotics and an extracellular proteolytic complex. The aim of this work was to establish the influence of transcription and translation inhibitors on biosynthesis of polyene antibiotic and proteolytic enzymes. The inhibitors were added at inoculation and at specific time intervals of fermentation. The production of hexaene H-85 is susceptible to rifamycin (transcription inhibitor) and chloramphenicol (translation inhibitor). Rifamycin reduces the production and trypsin like protease (TLP) and aminopeptidase (AP). while chloramphenicol acts only on TLP. Simultaneously these inhibitors almost have no effect on strain growth., Streptomicete su gram pozitivne bakterije čije je najvažnije stanište zemlja. Pri rastu na čvrstim podlogama i u submerznim uslovima grade supstratni i vazdušni micelijum. Morfološka diferencijacija je praćena produkcijom sekundarnih metabolita kao što su antibiotici, a i različitih hidrolitičkih enzima. Za mikroorganizme koji ih proizvode antibiotici predstavljaju kompetitivno oružje, agense za transport metala kao i efektore diferencijacije. Proteolitički enzimi, takođe, imaju određenu ulogu u razvojnom ciklusu aktinomiceta. Na primer, uključeni su u procese građenja vazdušnog micelija i sporulaciju. Soj Streptomyces hygroscopicus CH-7 pri fermentaciji proizvodi antibiotike poliketidne strukture i smesu ekstracelularnih proteolitičkih enzima. U ovom radu je ispitivan uticaj antibiotika rifamicina i hloramfenikola, inhibitora transkripcije i translacije, na produkciju polienskog antibiotika heksaena H-85 i proteolitičkih enzima. Inhibited su dodavani pri inokulaciji (O h) i u različitim vremenskim intervalima fermentacije (12-48 h). Dejstvom antibiotika inhibitora transkripcije i translacije utvrdjeno je da se enzim polien sintetaza transkribuje do 12. časa, a translacija teče do 48. časa fermentacije. Dodatak rifamicina pri zasejavanju drastično snižava proteolitičku aktivnost, a u kasnijim fazama izaziva smanjenje (36-80%). Hloramfenikol smanjuje produkciju tripsinu slične proteaze. ali nema dejstvo na aminopeptidaznu aktivnost. Rifamicin i hloramfenikol ne pokazuju značajno inhibitorno dejstvo na rast soja.",
journal = "Acta biologica iugoslavica - serija B: Mikrobiologija",
title = "The effect of rifamycin and chloramphenicol on antibiotic and protease production by Streptomyces hygroscopicus CH-7, Dejstvo rifamicina i hloramfenikola na produkciju antibiotika i proteaza soja Streptomyces hygroscopicus CH-7",
volume = "43",
number = "1",
pages = "31-40",
url = "https://hdl.handle.net/21.15107/rcub_cer_228"
}

Gojgić-Cvijović, G., Karadžić, I., Gopčević, K.,& Borozan, S.. (2006). The effect of rifamycin and chloramphenicol on antibiotic and protease production by Streptomyces hygroscopicus CH-7. in Acta biologica iugoslavica - serija B: Mikrobiologija, 43(1), 31-40.
https://hdl.handle.net/21.15107/rcub_cer_228

Gojgić-Cvijović G, Karadžić I, Gopčević K, Borozan S. The effect of rifamycin and chloramphenicol on antibiotic and protease production by Streptomyces hygroscopicus CH-7. in Acta biologica iugoslavica - serija B: Mikrobiologija. 2006;43(1):31-40.
https://hdl.handle.net/21.15107/rcub_cer_228 .

Gojgić-Cvijović, Gordana, Karadžić, Ivanka, Gopčević, Kristina, Borozan, Sunčica, "The effect of rifamycin and chloramphenicol on antibiotic and protease production by Streptomyces hygroscopicus CH-7" in Acta biologica iugoslavica - serija B: Mikrobiologija, 43, no. 1 (2006):31-40,
https://hdl.handle.net/21.15107/rcub_cer_228 .

TY  - CONF
AU  - Stojanović, Srđan
AU  - Borozan, Sunčica
AU  - Rebić, Radmila
AU  - Đurđić, Vidosava
AU  - Đorđević, Predrag
PY  - 1996
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6550
AB  - Insulinomas are pancreatic tumors associated with hyperinsulinism and hypoglycaemia. The effects of long-term hypoglycaemia, caused by hyperinsulinism in insulinoma patients, on erythrocyte metabolism, were in­vestigated. The modifications of erythrocyte metabolism were studied in prolonged hypoglycaemia. “Fast” haemoglobin modifications were re-invcstigated and the activi­ties in some erythrocyte enzymes of carbohydrate metab­olism and oxidative defense system, were determined. On the basis of the results obtained the structural alterations of haemoglobin molecule and the physiological impor­ tance of these changes, were assumed.
PB  - Bulgaria : Balkan Federation of Clinical Laboratory
C3  - Balkan Journal of Clinical Laboratory
T1  - Erythrocyte metabolism in insulin-induced hypoglycemia
VL  - 3
IS  - 1
SP  - 163
EP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_cer_6550
ER  - 

@conference{
author = "Stojanović, Srđan and Borozan, Sunčica and Rebić, Radmila and Đurđić, Vidosava and Đorđević, Predrag",
year = "1996",
abstract = "Insulinomas are pancreatic tumors associated with hyperinsulinism and hypoglycaemia. The effects of long-term hypoglycaemia, caused by hyperinsulinism in insulinoma patients, on erythrocyte metabolism, were in­vestigated. The modifications of erythrocyte metabolism were studied in prolonged hypoglycaemia. “Fast” haemoglobin modifications were re-invcstigated and the activi­ties in some erythrocyte enzymes of carbohydrate metab­olism and oxidative defense system, were determined. On the basis of the results obtained the structural alterations of haemoglobin molecule and the physiological impor­ tance of these changes, were assumed.",
publisher = "Bulgaria : Balkan Federation of Clinical Laboratory",
journal = "Balkan Journal of Clinical Laboratory",
title = "Erythrocyte metabolism in insulin-induced hypoglycemia",
volume = "3",
number = "1",
pages = "163-163",
url = "https://hdl.handle.net/21.15107/rcub_cer_6550"
}

Stojanović, S., Borozan, S., Rebić, R., Đurđić, V.,& Đorđević, P.. (1996). Erythrocyte metabolism in insulin-induced hypoglycemia. in Balkan Journal of Clinical Laboratory
Bulgaria : Balkan Federation of Clinical Laboratory., 3(1), 163-163.
https://hdl.handle.net/21.15107/rcub_cer_6550

Stojanović S, Borozan S, Rebić R, Đurđić V, Đorđević P. Erythrocyte metabolism in insulin-induced hypoglycemia. in Balkan Journal of Clinical Laboratory. 1996;3(1):163-163.
https://hdl.handle.net/21.15107/rcub_cer_6550 .

Stojanović, Srđan, Borozan, Sunčica, Rebić, Radmila, Đurđić, Vidosava, Đorđević, Predrag, "Erythrocyte metabolism in insulin-induced hypoglycemia" in Balkan Journal of Clinical Laboratory, 3, no. 1 (1996):163-163,
https://hdl.handle.net/21.15107/rcub_cer_6550 .

TY  - CONF
AU  - Rebić, Radmila
AU  - Rebić, Petar
AU  - Đurđić, Vidosava
AU  - Borozan, Sunčica
AU  - Todorović, S.
AU  - Stojanović, Srđan
PY  - 1996
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6549
AB  - ln study anti-oxidative enzymes (Cat, G-Px, G-red, G-6PD11 and GSH) in patients with pulmonary emphysema, we analyzed the blood samples taken from 20 patients with global respiratory failure (10 males, mean age 56+-7.5 yis, and 10 females, mean age 60±5yrs). All patients were heavy cigarette smokers, in average 33±8 pack-years. Chest radiographs of these patients were compatible with lung emphysema. Spirometric examinations revealed severe, irreversible pressure-dependent airflow limitations; body plethysmographic measurements revealed overinflation in increased total lung capacities, and transfer factors were greatly reduced. The control group consisted of 14 healthy smokers. Slightly elevated (alpha-1-antitrypsin in our pts., 3.55±0.76 g/L, indicated an acute phase reaction. The highly positive correla­tion was found between Cu and LDH (r=0.81, p<0.001) and between Cu and Mg (r=0.47, p<0.01). These results are correlated to the parameters of acid - base status, and they suggest that it is necessary to determine copper in the blood of patients with COLD in order to correctly evaluate hypoxia.
PB  - Bulgaria : Balkan Federation of Clinical Laboratory
C3  - Balkan Journal of Clinical Laboratory
T1  - Anti-oxidative enzymes in pulmonary emphysemia
VL  - 3
IS  - 1
SP  - 148
EP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_cer_6549
ER  - 

@conference{
author = "Rebić, Radmila and Rebić, Petar and Đurđić, Vidosava and Borozan, Sunčica and Todorović, S. and Stojanović, Srđan",
year = "1996",
abstract = "ln study anti-oxidative enzymes (Cat, G-Px, G-red, G-6PD11 and GSH) in patients with pulmonary emphysema, we analyzed the blood samples taken from 20 patients with global respiratory failure (10 males, mean age 56+-7.5 yis, and 10 females, mean age 60±5yrs). All patients were heavy cigarette smokers, in average 33±8 pack-years. Chest radiographs of these patients were compatible with lung emphysema. Spirometric examinations revealed severe, irreversible pressure-dependent airflow limitations; body plethysmographic measurements revealed overinflation in increased total lung capacities, and transfer factors were greatly reduced. The control group consisted of 14 healthy smokers. Slightly elevated (alpha-1-antitrypsin in our pts., 3.55±0.76 g/L, indicated an acute phase reaction. The highly positive correla­tion was found between Cu and LDH (r=0.81, p<0.001) and between Cu and Mg (r=0.47, p<0.01). These results are correlated to the parameters of acid - base status, and they suggest that it is necessary to determine copper in the blood of patients with COLD in order to correctly evaluate hypoxia.",
publisher = "Bulgaria : Balkan Federation of Clinical Laboratory",
journal = "Balkan Journal of Clinical Laboratory",
title = "Anti-oxidative enzymes in pulmonary emphysemia",
volume = "3",
number = "1",
pages = "148-149",
url = "https://hdl.handle.net/21.15107/rcub_cer_6549"
}

Rebić, R., Rebić, P., Đurđić, V., Borozan, S., Todorović, S.,& Stojanović, S.. (1996). Anti-oxidative enzymes in pulmonary emphysemia. in Balkan Journal of Clinical Laboratory
Bulgaria : Balkan Federation of Clinical Laboratory., 3(1), 148-149.
https://hdl.handle.net/21.15107/rcub_cer_6549

Rebić R, Rebić P, Đurđić V, Borozan S, Todorović S, Stojanović S. Anti-oxidative enzymes in pulmonary emphysemia. in Balkan Journal of Clinical Laboratory. 1996;3(1):148-149.
https://hdl.handle.net/21.15107/rcub_cer_6549 .

Rebić, Radmila, Rebić, Petar, Đurđić, Vidosava, Borozan, Sunčica, Todorović, S., Stojanović, Srđan, "Anti-oxidative enzymes in pulmonary emphysemia" in Balkan Journal of Clinical Laboratory, 3, no. 1 (1996):148-149,
https://hdl.handle.net/21.15107/rcub_cer_6549 .

TY  - CONF
AU  - Rebić, Radmila
AU  - Rebić, Petar
AU  - Đurđić, Vidosava
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
AU  - Todorović, S.
PY  - 1996
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6541
AB  - We analyzed the blood samples from 20 patients with global respiratory failure (10 males, mean age 56±7 yrs. and 10 females, mean age 60±5 yrs.). All patients were heavy cigarette smokers, in average 33±8 pack-years.
Spirometrics examinations suggested severe, irreversible pressure dependent airflow limitations; body plethysmo­graphic measurements revealed overinflalion in increased total lung capacities, and transfer factors were greatly reduced. The control group consisted of 20 healthy smokers.
Slightly elevated alfa 1-PI in our patients, 3.55±0.76 g/L, indicated an acute phase reaction. As compared to the control group, we found significantly decreased concentration of zinc (74±24 : 105±34 pg/100 niL, p<0.001).
The mean values of ceruloplasmin were similar in the two groups (0.45±0.01 ; 0.37±0.05 g/L). Scrum copper levels were also similar in both groups (126±46 : 134±14pg/100mL). The other authors have stated that in inflam­mation the copper concentration was increased. However our findings could not prove it. Decreased concentration of zinc pointed to the destabilization of lysosomal mem­ branes and increased macrophage activity that liberates proteases.
PB  - Bulgaria : Balkan Federation of Clinical Laboratory
C3  - Balkan Journal of Clinical Laboratory
T1  - Ceruloplasmin, copper and zinc in pulmonary emphysemia
VL  - 3
IS  - 1
SP  - 157
EP  - 157
UR  - https://hdl.handle.net/21.15107/rcub_cer_6541
ER  - 

@conference{
author = "Rebić, Radmila and Rebić, Petar and Đurđić, Vidosava and Borozan, Sunčica and Stojanović, Srđan and Todorović, S.",
year = "1996",
abstract = "We analyzed the blood samples from 20 patients with global respiratory failure (10 males, mean age 56±7 yrs. and 10 females, mean age 60±5 yrs.). All patients were heavy cigarette smokers, in average 33±8 pack-years.
Spirometrics examinations suggested severe, irreversible pressure dependent airflow limitations; body plethysmo­graphic measurements revealed overinflalion in increased total lung capacities, and transfer factors were greatly reduced. The control group consisted of 20 healthy smokers.
Slightly elevated alfa 1-PI in our patients, 3.55±0.76 g/L, indicated an acute phase reaction. As compared to the control group, we found significantly decreased concentration of zinc (74±24 : 105±34 pg/100 niL, p<0.001).
The mean values of ceruloplasmin were similar in the two groups (0.45±0.01 ; 0.37±0.05 g/L). Scrum copper levels were also similar in both groups (126±46 : 134±14pg/100mL). The other authors have stated that in inflam­mation the copper concentration was increased. However our findings could not prove it. Decreased concentration of zinc pointed to the destabilization of lysosomal mem­ branes and increased macrophage activity that liberates proteases.",
publisher = "Bulgaria : Balkan Federation of Clinical Laboratory",
journal = "Balkan Journal of Clinical Laboratory",
title = "Ceruloplasmin, copper and zinc in pulmonary emphysemia",
volume = "3",
number = "1",
pages = "157-157",
url = "https://hdl.handle.net/21.15107/rcub_cer_6541"
}

Rebić, R., Rebić, P., Đurđić, V., Borozan, S., Stojanović, S.,& Todorović, S.. (1996). Ceruloplasmin, copper and zinc in pulmonary emphysemia. in Balkan Journal of Clinical Laboratory
Bulgaria : Balkan Federation of Clinical Laboratory., 3(1), 157-157.
https://hdl.handle.net/21.15107/rcub_cer_6541

Rebić R, Rebić P, Đurđić V, Borozan S, Stojanović S, Todorović S. Ceruloplasmin, copper and zinc in pulmonary emphysemia. in Balkan Journal of Clinical Laboratory. 1996;3(1):157-157.
https://hdl.handle.net/21.15107/rcub_cer_6541 .

Rebić, Radmila, Rebić, Petar, Đurđić, Vidosava, Borozan, Sunčica, Stojanović, Srđan, Todorović, S., "Ceruloplasmin, copper and zinc in pulmonary emphysemia" in Balkan Journal of Clinical Laboratory, 3, no. 1 (1996):157-157,
https://hdl.handle.net/21.15107/rcub_cer_6541 .

TY  - CONF
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
AU  - Đurđić, Vidosava
PY  - 1995
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6527
AB  - In this work the effect o f toluene exposition on oxidative chemical species generation, metabolic alteration and protective effect of selenium were investigated.
The disturbances in erythrocyte metabolism due to oxidative processes were studied by the use o f experimental animals. The alterations of some erythrocyte enzyme activities and antioxidative molecules were found in exposed to toluene. Also, the more intensive primary antioxidative response to oxidative stress and the protective effect of selenium was detected.
PB  - Timisoara, Romania : Publishing House "Mirton" Timisoara
C3  - Proceedings of the International Symposium on Metal Elements in Environment, Medicine and Biology, October 21-23, 1995, Timisoara, Romania
T1  - The influence of selenium on the erythrocyte metabolism in rats exposed to toluene. II Autooxidative response to oxidative stress
SP  - 253
EP  - 256
UR  - https://hdl.handle.net/21.15107/rcub_cer_6527
ER  - 

@conference{
author = "Borozan, Sunčica and Stojanović, Srđan and Đurđić, Vidosava",
year = "1995",
abstract = "In this work the effect o f toluene exposition on oxidative chemical species generation, metabolic alteration and protective effect of selenium were investigated.
The disturbances in erythrocyte metabolism due to oxidative processes were studied by the use o f experimental animals. The alterations of some erythrocyte enzyme activities and antioxidative molecules were found in exposed to toluene. Also, the more intensive primary antioxidative response to oxidative stress and the protective effect of selenium was detected.",
publisher = "Timisoara, Romania : Publishing House "Mirton" Timisoara",
journal = "Proceedings of the International Symposium on Metal Elements in Environment, Medicine and Biology, October 21-23, 1995, Timisoara, Romania",
title = "The influence of selenium on the erythrocyte metabolism in rats exposed to toluene. II Autooxidative response to oxidative stress",
pages = "253-256",
url = "https://hdl.handle.net/21.15107/rcub_cer_6527"
}

Borozan, S., Stojanović, S.,& Đurđić, V.. (1995). The influence of selenium on the erythrocyte metabolism in rats exposed to toluene. II Autooxidative response to oxidative stress. in Proceedings of the International Symposium on Metal Elements in Environment, Medicine and Biology, October 21-23, 1995, Timisoara, Romania
Timisoara, Romania : Publishing House "Mirton" Timisoara., 253-256.
https://hdl.handle.net/21.15107/rcub_cer_6527

Borozan S, Stojanović S, Đurđić V. The influence of selenium on the erythrocyte metabolism in rats exposed to toluene. II Autooxidative response to oxidative stress. in Proceedings of the International Symposium on Metal Elements in Environment, Medicine and Biology, October 21-23, 1995, Timisoara, Romania. 1995;:253-256.
https://hdl.handle.net/21.15107/rcub_cer_6527 .

Borozan, Sunčica, Stojanović, Srđan, Đurđić, Vidosava, "The influence of selenium on the erythrocyte metabolism in rats exposed to toluene. II Autooxidative response to oxidative stress" in Proceedings of the International Symposium on Metal Elements in Environment, Medicine and Biology, October 21-23, 1995, Timisoara, Romania (1995):253-256,
https://hdl.handle.net/21.15107/rcub_cer_6527 .

TY  - CONF
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
AU  - Đurđić, Vidosava
PY  - 1995
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6526
AB  - In this work unusual hemoglobin modifications were detected in hemolysates of experimental animals exposed to toluene. The protective effect of selenium administration against hemoglobin structural alterations was investigated too. On the basis of antioxidant enzyme activities, it was concluded that a new hemoglobin fraction found in animals exposed to toluene is hemoglobin modification due to the oxidative chemical species generation.
PB  - Timisoara, Romania : Publishing House "Mirton" Timisoara
C3  - Proceedings of the International Symposium on Metal Elements in Environment, Medicine and Biology, October 21-23, 1995, Timisoara, Romania
T1  - The influence of selenium on the erythrocyte metabolism in rats exposed to toluene. I Synthesis of unusual hemoglobin modifications
SP  - 249
EP  - 252
UR  - https://hdl.handle.net/21.15107/rcub_cer_6526
ER  - 

@conference{
author = "Borozan, Sunčica and Stojanović, Srđan and Đurđić, Vidosava",
year = "1995",
abstract = "In this work unusual hemoglobin modifications were detected in hemolysates of experimental animals exposed to toluene. The protective effect of selenium administration against hemoglobin structural alterations was investigated too. On the basis of antioxidant enzyme activities, it was concluded that a new hemoglobin fraction found in animals exposed to toluene is hemoglobin modification due to the oxidative chemical species generation.",
publisher = "Timisoara, Romania : Publishing House "Mirton" Timisoara",
journal = "Proceedings of the International Symposium on Metal Elements in Environment, Medicine and Biology, October 21-23, 1995, Timisoara, Romania",
title = "The influence of selenium on the erythrocyte metabolism in rats exposed to toluene. I Synthesis of unusual hemoglobin modifications",
pages = "249-252",
url = "https://hdl.handle.net/21.15107/rcub_cer_6526"
}

Borozan, S., Stojanović, S.,& Đurđić, V.. (1995). The influence of selenium on the erythrocyte metabolism in rats exposed to toluene. I Synthesis of unusual hemoglobin modifications. in Proceedings of the International Symposium on Metal Elements in Environment, Medicine and Biology, October 21-23, 1995, Timisoara, Romania
Timisoara, Romania : Publishing House "Mirton" Timisoara., 249-252.
https://hdl.handle.net/21.15107/rcub_cer_6526

Borozan S, Stojanović S, Đurđić V. The influence of selenium on the erythrocyte metabolism in rats exposed to toluene. I Synthesis of unusual hemoglobin modifications. in Proceedings of the International Symposium on Metal Elements in Environment, Medicine and Biology, October 21-23, 1995, Timisoara, Romania. 1995;:249-252.
https://hdl.handle.net/21.15107/rcub_cer_6526 .

Borozan, Sunčica, Stojanović, Srđan, Đurđić, Vidosava, "The influence of selenium on the erythrocyte metabolism in rats exposed to toluene. I Synthesis of unusual hemoglobin modifications" in Proceedings of the International Symposium on Metal Elements in Environment, Medicine and Biology, October 21-23, 1995, Timisoara, Romania (1995):249-252,
https://hdl.handle.net/21.15107/rcub_cer_6526 .

TY  - CONF
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
AU  - Đurđić, Vidosava
AU  - Đujić, Ivana
PY  - 1992
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6591
AB  - Slobodni kiseonični radikali su izuzetno reaktivan hemijska jedinjenja. Kao takvi lako mogu dovesti do oštećenja različitih biomolekula enzima, ćelijskih mem­ brana, pa im se pripisuje potencijalna uloga u genezi mnogih patoloških procesa. Izlaganje dejstvu aroma­ tičnih organskih rastvarača može dovesti do stvaranja većih količina slobodnih kiseoničnih radikala. U ovome radu ispitivan je efekat izlaganja dejstvu aromatičnih organskih rastvarača i slobodnih kiseoničnih radikala na metabolizam u eritrocitima. Analizirani su indeksi antioksidativnog statusa eritrocita, sadržaj glutationa i antioksidativnih enzima (glutationperoksidaze i reduk- taze, katalaze i superoksidne dizmutaze). Takođe je ispitan efekat oskidativnih radikala na metabolizam hemoglobina i ugljenih hidrata. Određena je aktivnost methemoglobin reduktaze i sadržaj hemoglobinskih derivata.
AB  - Free radicals are extremly reactive chemical species. Damaging various biomolecules, enzymes, cell membranes they have a potential role in many pathological processes. Exposition to aromatic organic solvents may lead to increase generation of free radicals. The effect of aromatic organic solvents and generated free radicals on erythrocyte metabolism are investigated. The indices of antioxidant status in erythrocytes, glutathione and antioxydant enzymes (glutathione peroxidase and reductase, catalase and superoxidismutase) were investigated. The effect of oxidative radicals upon hemoglobin and carbohydrate metabolism was investigated too. Hemoglobin modifications and derivatives, as well as activity of methemoglobin reductase was analysed as well as the activities of key anzyrnes of pentose phosphate shunt.
PB  - Društvo medicinskih biohemičara Jugoslavije
C3  - Jugoslovenska medicinska biohemija / Yugoslav Medical Biochemistry
T1  - Uticaj aromatičnih organskih rastvarača na antioksidativni sistem u eritrocitima
T1  - The influence of aromatic organic solvents on the antioxidative system in erythrocytes
VL  - 11
IS  - 3-4
SP  - 167
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_cer_6591
ER  - 

@conference{
author = "Borozan, Sunčica and Stojanović, Srđan and Đurđić, Vidosava and Đujić, Ivana",
year = "1992",
abstract = "Slobodni kiseonični radikali su izuzetno reaktivan hemijska jedinjenja. Kao takvi lako mogu dovesti do oštećenja različitih biomolekula enzima, ćelijskih mem­ brana, pa im se pripisuje potencijalna uloga u genezi mnogih patoloških procesa. Izlaganje dejstvu aroma­ tičnih organskih rastvarača može dovesti do stvaranja većih količina slobodnih kiseoničnih radikala. U ovome radu ispitivan je efekat izlaganja dejstvu aromatičnih organskih rastvarača i slobodnih kiseoničnih radikala na metabolizam u eritrocitima. Analizirani su indeksi antioksidativnog statusa eritrocita, sadržaj glutationa i antioksidativnih enzima (glutationperoksidaze i reduk- taze, katalaze i superoksidne dizmutaze). Takođe je ispitan efekat oskidativnih radikala na metabolizam hemoglobina i ugljenih hidrata. Određena je aktivnost methemoglobin reduktaze i sadržaj hemoglobinskih derivata., Free radicals are extremly reactive chemical species. Damaging various biomolecules, enzymes, cell membranes they have a potential role in many pathological processes. Exposition to aromatic organic solvents may lead to increase generation of free radicals. The effect of aromatic organic solvents and generated free radicals on erythrocyte metabolism are investigated. The indices of antioxidant status in erythrocytes, glutathione and antioxydant enzymes (glutathione peroxidase and reductase, catalase and superoxidismutase) were investigated. The effect of oxidative radicals upon hemoglobin and carbohydrate metabolism was investigated too. Hemoglobin modifications and derivatives, as well as activity of methemoglobin reductase was analysed as well as the activities of key anzyrnes of pentose phosphate shunt.",
publisher = "Društvo medicinskih biohemičara Jugoslavije",
journal = "Jugoslovenska medicinska biohemija / Yugoslav Medical Biochemistry",
title = "Uticaj aromatičnih organskih rastvarača na antioksidativni sistem u eritrocitima, The influence of aromatic organic solvents on the antioxidative system in erythrocytes",
volume = "11",
number = "3-4",
pages = "167-167",
url = "https://hdl.handle.net/21.15107/rcub_cer_6591"
}

Borozan, S., Stojanović, S., Đurđić, V.,& Đujić, I.. (1992). Uticaj aromatičnih organskih rastvarača na antioksidativni sistem u eritrocitima. in Jugoslovenska medicinska biohemija / Yugoslav Medical Biochemistry
Društvo medicinskih biohemičara Jugoslavije., 11(3-4), 167-167.
https://hdl.handle.net/21.15107/rcub_cer_6591

Borozan S, Stojanović S, Đurđić V, Đujić I. Uticaj aromatičnih organskih rastvarača na antioksidativni sistem u eritrocitima. in Jugoslovenska medicinska biohemija / Yugoslav Medical Biochemistry. 1992;11(3-4):167-167.
https://hdl.handle.net/21.15107/rcub_cer_6591 .

Borozan, Sunčica, Stojanović, Srđan, Đurđić, Vidosava, Đujić, Ivana, "Uticaj aromatičnih organskih rastvarača na antioksidativni sistem u eritrocitima" in Jugoslovenska medicinska biohemija / Yugoslav Medical Biochemistry, 11, no. 3-4 (1992):167-167,
https://hdl.handle.net/21.15107/rcub_cer_6591 .

TY  - CONF
AU  - Stojanović, Srđan
AU  - Borozan, Sunčica
AU  - Frim, Olga
AU  - Đujić, Ivana
PY  - 1991
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6551
AB  - The aim of this work was the investigation of the influence of ionizing radiation on the erythrocyte antioxidant system and metabolism. The erythrocyte antioxidative enzymes, GSH/GSSG ratio, as well as selenium concentrations were analysed in the same time with the main enzyme involved in carbohydrate and hemoglobin metabolism. The amounts of hemoglobin derivatives and modifications were investigated, too. The analysis was performed using erythrocytes of a group of patients with cancer bring exposed to ionizing radiation or not as well as healthy control in same conditions.
On the basis of results the inhibitory effects of ionizing radiation upon near all enzyme activities were detected as well as the corresponding alterations in efficiency of antioxidant system, carbohydrate and hemoglobin metabolism.
PB  - The Israel Society for Biochemistry and Molecular Biology
C3  - Abstracts - 15th International Congress of Biochemistry, August 4-8, 1991, Jerusalem, Israel
T1  - Antioxidant system in human erythrocytes involved in radioprotection
SP  - 267
EP  - 267
UR  - https://hdl.handle.net/21.15107/rcub_cer_6551
ER  - 

@conference{
author = "Stojanović, Srđan and Borozan, Sunčica and Frim, Olga and Đujić, Ivana",
year = "1991",
abstract = "The aim of this work was the investigation of the influence of ionizing radiation on the erythrocyte antioxidant system and metabolism. The erythrocyte antioxidative enzymes, GSH/GSSG ratio, as well as selenium concentrations were analysed in the same time with the main enzyme involved in carbohydrate and hemoglobin metabolism. The amounts of hemoglobin derivatives and modifications were investigated, too. The analysis was performed using erythrocytes of a group of patients with cancer bring exposed to ionizing radiation or not as well as healthy control in same conditions.
On the basis of results the inhibitory effects of ionizing radiation upon near all enzyme activities were detected as well as the corresponding alterations in efficiency of antioxidant system, carbohydrate and hemoglobin metabolism.",
publisher = "The Israel Society for Biochemistry and Molecular Biology",
journal = "Abstracts - 15th International Congress of Biochemistry, August 4-8, 1991, Jerusalem, Israel",
title = "Antioxidant system in human erythrocytes involved in radioprotection",
pages = "267-267",
url = "https://hdl.handle.net/21.15107/rcub_cer_6551"
}

Stojanović, S., Borozan, S., Frim, O.,& Đujić, I.. (1991). Antioxidant system in human erythrocytes involved in radioprotection. in Abstracts - 15th International Congress of Biochemistry, August 4-8, 1991, Jerusalem, Israel
The Israel Society for Biochemistry and Molecular Biology., 267-267.
https://hdl.handle.net/21.15107/rcub_cer_6551

Stojanović S, Borozan S, Frim O, Đujić I. Antioxidant system in human erythrocytes involved in radioprotection. in Abstracts - 15th International Congress of Biochemistry, August 4-8, 1991, Jerusalem, Israel. 1991;:267-267.
https://hdl.handle.net/21.15107/rcub_cer_6551 .

Stojanović, Srđan, Borozan, Sunčica, Frim, Olga, Đujić, Ivana, "Antioxidant system in human erythrocytes involved in radioprotection" in Abstracts - 15th International Congress of Biochemistry, August 4-8, 1991, Jerusalem, Israel (1991):267-267,
https://hdl.handle.net/21.15107/rcub_cer_6551 .

TY  - CONF
AU  - Mandić, Ljuba
AU  - Đurđić, Vidosava
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
PY  - 1990
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6552
AB  - Insulinoma, pancreas tumors lead to the increased insulin secretion, glucose consump­tion and the hypoglycemia emergence. The changes of erythrocyte metabolism in hypoglycemia have not been studied so far.
To explain the influence of hypoglycemia on erythrocyte metabolism the activities of erythrocyte enzymes and the content of hemo­globin derivates were analyzed. More precise identification of some modified hemoglobins was carried out; and their contents we­re analysed too.The physiological importance and practical application of results remains to be solved.
PB  - The Federation of European Biochemical Societies (FEBS)
C3  - Abstracts - 20th Meeting of the Federation of European Biochemical Societies, August 19-24, 1990, Budapest, Hungary
T1  - The changes in erythrocyte metabolism in hypoglycemia
SP  - 149
EP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_cer_6552
ER  - 

@conference{
author = "Mandić, Ljuba and Đurđić, Vidosava and Borozan, Sunčica and Stojanović, Srđan",
year = "1990",
abstract = "Insulinoma, pancreas tumors lead to the increased insulin secretion, glucose consump­tion and the hypoglycemia emergence. The changes of erythrocyte metabolism in hypoglycemia have not been studied so far.
To explain the influence of hypoglycemia on erythrocyte metabolism the activities of erythrocyte enzymes and the content of hemo­globin derivates were analyzed. More precise identification of some modified hemoglobins was carried out; and their contents we­re analysed too.The physiological importance and practical application of results remains to be solved.",
publisher = "The Federation of European Biochemical Societies (FEBS)",
journal = "Abstracts - 20th Meeting of the Federation of European Biochemical Societies, August 19-24, 1990, Budapest, Hungary",
title = "The changes in erythrocyte metabolism in hypoglycemia",
pages = "149-149",
url = "https://hdl.handle.net/21.15107/rcub_cer_6552"
}

Mandić, L., Đurđić, V., Borozan, S.,& Stojanović, S.. (1990). The changes in erythrocyte metabolism in hypoglycemia. in Abstracts - 20th Meeting of the Federation of European Biochemical Societies, August 19-24, 1990, Budapest, Hungary
The Federation of European Biochemical Societies (FEBS)., 149-149.
https://hdl.handle.net/21.15107/rcub_cer_6552

Mandić L, Đurđić V, Borozan S, Stojanović S. The changes in erythrocyte metabolism in hypoglycemia. in Abstracts - 20th Meeting of the Federation of European Biochemical Societies, August 19-24, 1990, Budapest, Hungary. 1990;:149-149.
https://hdl.handle.net/21.15107/rcub_cer_6552 .

Mandić, Ljuba, Đurđić, Vidosava, Borozan, Sunčica, Stojanović, Srđan, "The changes in erythrocyte metabolism in hypoglycemia" in Abstracts - 20th Meeting of the Federation of European Biochemical Societies, August 19-24, 1990, Budapest, Hungary (1990):149-149,
https://hdl.handle.net/21.15107/rcub_cer_6552 .