TY  - JOUR
AU  - Miljković, Miona G.
AU  - Davidović, Slađana Z.
AU  - Djukić˗Vuković, Aleksandra P.
AU  - Ilić, Mila
AU  - Simović, Milica B.
AU  - Rajilić-Stojanović, Mirjana D.
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4790
AB  - Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391±0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.
AB  - Dekstransaharaza (DS) je glukoziltransferaza (E. C. 2.4.1.5.) koja katalizuje prenos ostataka glukoze iz saharoze u polimer dekstrana, pri čemu se oslobađa fruktoza. Ovaj enzim je povezan sa širokim spektrom primene dekstrana i oligosaharida. Proizvodnja dekstransaharaze pomoću bakterije Leuconostoc mesenteroides T3 optimizovana je metodom odzivnih površina korišćenjem centralnog kompozitnog dizajna. Za optimizaciju su izabrane tri promenljive: koncentracija džibre, koncentracija saharoze i koncentracija jona mangana. Rezultati su pokazali da koncentracije saharoze i jona mangana imaju pozitivan linearni efekat na proizvodnju DS dok su sve interakcije (džibra-Mn2+, džibra-saharoza i saharoza-Mn2+) imale značajan uticaj na proizvodnju DS. Na osnovu eksperimentalnih podataka i numeričke optimizacije, dobijen je maksimalni prinos DS od 3.391 ± 0.131 U cm-3 u podlozi sa 64.33 % džibre, 5.30 % saharoze i 0.022 % jona mangana. Naše istraživanje otkrilo je da se džibra u kombinaciji sa melasom šećerne repe kao i saharozom i dodatkom jona mangana može koristiti kao dragocena hranjiva komponenta za rast bakterija mlečne kiseline i proizvodnju DS. Takođe, uzimajući u obzir poreklo supstrata, upotreba industrijskih nusproizvoda na ovaj način ima veliku ekološku važnost.
PB  - Belgrade : Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Utilization of agro-industrial by-products as substrates for dextransucrase production by leuconostoc mesenteroides t3: Process optimization using response surface methodology
T1  - Iskorišćenje nus-proizvoda agro-industrije za proizvodnju dekstransaharaze pomoću bakterije leuconostoc mesenteroides t3: Optimizacija procesa metodom odzivnih površina
VL  - 75
IS  - 3
SP  - 135
EP  - 146
DO  - 10.2298/HEMIND200710015M
ER  - 

@article{
author = "Miljković, Miona G. and Davidović, Slađana Z. and Djukić˗Vuković, Aleksandra P. and Ilić, Mila and Simović, Milica B. and Rajilić-Stojanović, Mirjana D. and Dimitrijević-Branković, Suzana I.",
year = "2021",
abstract = "Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391±0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy., Dekstransaharaza (DS) je glukoziltransferaza (E. C. 2.4.1.5.) koja katalizuje prenos ostataka glukoze iz saharoze u polimer dekstrana, pri čemu se oslobađa fruktoza. Ovaj enzim je povezan sa širokim spektrom primene dekstrana i oligosaharida. Proizvodnja dekstransaharaze pomoću bakterije Leuconostoc mesenteroides T3 optimizovana je metodom odzivnih površina korišćenjem centralnog kompozitnog dizajna. Za optimizaciju su izabrane tri promenljive: koncentracija džibre, koncentracija saharoze i koncentracija jona mangana. Rezultati su pokazali da koncentracije saharoze i jona mangana imaju pozitivan linearni efekat na proizvodnju DS dok su sve interakcije (džibra-Mn2+, džibra-saharoza i saharoza-Mn2+) imale značajan uticaj na proizvodnju DS. Na osnovu eksperimentalnih podataka i numeričke optimizacije, dobijen je maksimalni prinos DS od 3.391 ± 0.131 U cm-3 u podlozi sa 64.33 % džibre, 5.30 % saharoze i 0.022 % jona mangana. Naše istraživanje otkrilo je da se džibra u kombinaciji sa melasom šećerne repe kao i saharozom i dodatkom jona mangana može koristiti kao dragocena hranjiva komponenta za rast bakterija mlečne kiseline i proizvodnju DS. Takođe, uzimajući u obzir poreklo supstrata, upotreba industrijskih nusproizvoda na ovaj način ima veliku ekološku važnost.",
publisher = "Belgrade : Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Utilization of agro-industrial by-products as substrates for dextransucrase production by leuconostoc mesenteroides t3: Process optimization using response surface methodology, Iskorišćenje nus-proizvoda agro-industrije za proizvodnju dekstransaharaze pomoću bakterije leuconostoc mesenteroides t3: Optimizacija procesa metodom odzivnih površina",
volume = "75",
number = "3",
pages = "135-146",
doi = "10.2298/HEMIND200710015M"
}

Miljković, M. G., Davidović, S. Z., Djukić˗Vuković, A. P., Ilić, M., Simović, M. B., Rajilić-Stojanović, M. D.,& Dimitrijević-Branković, S. I.. (2021). Utilization of agro-industrial by-products as substrates for dextransucrase production by leuconostoc mesenteroides t3: Process optimization using response surface methodology. in Hemijska industrija
Belgrade : Association of the Chemical Engineers of Serbia., 75(3), 135-146.
https://doi.org/10.2298/HEMIND200710015M

Miljković MG, Davidović SZ, Djukić˗Vuković AP, Ilić M, Simović MB, Rajilić-Stojanović MD, Dimitrijević-Branković SI. Utilization of agro-industrial by-products as substrates for dextransucrase production by leuconostoc mesenteroides t3: Process optimization using response surface methodology. in Hemijska industrija. 2021;75(3):135-146.
doi:10.2298/HEMIND200710015M .

Miljković, Miona G., Davidović, Slađana Z., Djukić˗Vuković, Aleksandra P., Ilić, Mila, Simović, Milica B., Rajilić-Stojanović, Mirjana D., Dimitrijević-Branković, Suzana I., "Utilization of agro-industrial by-products as substrates for dextransucrase production by leuconostoc mesenteroides t3: Process optimization using response surface methodology" in Hemijska industrija, 75, no. 3 (2021):135-146,
https://doi.org/10.2298/HEMIND200710015M . .