TY  - JOUR
AU  - Medić, Ana
AU  - Huttmann, Nico
AU  - Lješević, Marija
AU  - Risha, Yousef
AU  - Berezovski, Maxim
AU  - Minić, Zoran
AU  - Karadžić, Ivanka
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5548
AB  - Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.
PB  - Elsevier
T2  - Microbiological Research
T1  - A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform
VL  - 259
SP  - 126998
DO  - 10.1016/j.micres.2022.126998
ER  - 

@article{
author = "Medić, Ana and Huttmann, Nico and Lješević, Marija and Risha, Yousef and Berezovski, Maxim and Minić, Zoran and Karadžić, Ivanka",
year = "2022",
abstract = "Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.",
publisher = "Elsevier",
journal = "Microbiological Research",
title = "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform",
volume = "259",
pages = "126998",
doi = "10.1016/j.micres.2022.126998"
}

Medić, A., Huttmann, N., Lješević, M., Risha, Y., Berezovski, M., Minić, Z.,& Karadžić, I.. (2022). A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research
Elsevier., 259, 126998.
https://doi.org/10.1016/j.micres.2022.126998

Medić A, Huttmann N, Lješević M, Risha Y, Berezovski M, Minić Z, Karadžić I. A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research. 2022;259:126998.
doi:10.1016/j.micres.2022.126998 .

Medić, Ana, Huttmann, Nico, Lješević, Marija, Risha, Yousef, Berezovski, Maxim, Minić, Zoran, Karadžić, Ivanka, "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform" in Microbiological Research, 259 (2022):126998,
https://doi.org/10.1016/j.micres.2022.126998 . .

TY  - JOUR
AU  - Medić, Ana
AU  - Huttmann, Nico
AU  - Lješević, Marija
AU  - Risha, Yousef
AU  - Berezovski, Maxim
AU  - Minić, Zoran
AU  - Karadžić, Ivanka
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5190
AB  - Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.
PB  - Elsevier
T2  - Microbiological Research
T1  - A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform
VL  - 259
SP  - 126998
DO  - 10.1016/j.micres.2022.126998
ER  - 

@article{
author = "Medić, Ana and Huttmann, Nico and Lješević, Marija and Risha, Yousef and Berezovski, Maxim and Minić, Zoran and Karadžić, Ivanka",
year = "2022",
abstract = "Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.",
publisher = "Elsevier",
journal = "Microbiological Research",
title = "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform",
volume = "259",
pages = "126998",
doi = "10.1016/j.micres.2022.126998"
}

Medić, A., Huttmann, N., Lješević, M., Risha, Y., Berezovski, M., Minić, Z.,& Karadžić, I.. (2022). A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research
Elsevier., 259, 126998.
https://doi.org/10.1016/j.micres.2022.126998

Medić A, Huttmann N, Lješević M, Risha Y, Berezovski M, Minić Z, Karadžić I. A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research. 2022;259:126998.
doi:10.1016/j.micres.2022.126998 .

Medić, Ana, Huttmann, Nico, Lješević, Marija, Risha, Yousef, Berezovski, Maxim, Minić, Zoran, Karadžić, Ivanka, "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform" in Microbiological Research, 259 (2022):126998,
https://doi.org/10.1016/j.micres.2022.126998 . .