TY  - JOUR
AU  - Khalife, Majd
AU  - Stanković, Dalibor
AU  - Stanković, Vesna
AU  - Danicka, Julia
AU  - Rizzotto, Francesco
AU  - Costache, Vlad
AU  - Schwok, Anny Slama
AU  - Gaudu, Philippe
AU  - Vidić, Jasmina
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6896
AB  - Vitamin K refers to a group of vitamins that play an important role in blood coagulation and regulation of bone and vascular metabolism. However, vitamin K3 may give severe side effects in animal and humans when improperly added to food and feed due to its toxicity. Here, an electrochemical biosensor, based on the YaiB NADPH-dependent quinone reductase from Lactococcus lactis (YaiB), was developed to achieve rapid and redox probe-free detection of vitamin K3. First, the ability of the carbon electrode to distinguish between 1,4-benzoquinone and hydroquinone was demonstrated. Then, we engineered YaiB to work as a bioreceptor immobilized at the electrode and its sensitivity and specificity to reduce vitamin K3 were demonstrated. Finally, to demonstrate the practical potential of the biosensor, we tested it directly in spiked milk samples, achieving 15-minute quantification of the vitamin K3. The limit of detection was 0.87 µM and 4.1 µM in buffer and milk, respectively.
PB  - Elsevier
T2  - Food Chemistry
T1  - Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3
VL  - 433
SP  - 137316
DO  - 10.1016/j.foodchem.2023.137316
ER  - 

@article{
author = "Khalife, Majd and Stanković, Dalibor and Stanković, Vesna and Danicka, Julia and Rizzotto, Francesco and Costache, Vlad and Schwok, Anny Slama and Gaudu, Philippe and Vidić, Jasmina",
year = "2024",
abstract = "Vitamin K refers to a group of vitamins that play an important role in blood coagulation and regulation of bone and vascular metabolism. However, vitamin K3 may give severe side effects in animal and humans when improperly added to food and feed due to its toxicity. Here, an electrochemical biosensor, based on the YaiB NADPH-dependent quinone reductase from Lactococcus lactis (YaiB), was developed to achieve rapid and redox probe-free detection of vitamin K3. First, the ability of the carbon electrode to distinguish between 1,4-benzoquinone and hydroquinone was demonstrated. Then, we engineered YaiB to work as a bioreceptor immobilized at the electrode and its sensitivity and specificity to reduce vitamin K3 were demonstrated. Finally, to demonstrate the practical potential of the biosensor, we tested it directly in spiked milk samples, achieving 15-minute quantification of the vitamin K3. The limit of detection was 0.87 µM and 4.1 µM in buffer and milk, respectively.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3",
volume = "433",
pages = "137316",
doi = "10.1016/j.foodchem.2023.137316"
}

Khalife, M., Stanković, D., Stanković, V., Danicka, J., Rizzotto, F., Costache, V., Schwok, A. S., Gaudu, P.,& Vidić, J.. (2024). Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3. in Food Chemistry
Elsevier., 433, 137316.
https://doi.org/10.1016/j.foodchem.2023.137316

Khalife M, Stanković D, Stanković V, Danicka J, Rizzotto F, Costache V, Schwok AS, Gaudu P, Vidić J. Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3. in Food Chemistry. 2024;433:137316.
doi:10.1016/j.foodchem.2023.137316 .

Khalife, Majd, Stanković, Dalibor, Stanković, Vesna, Danicka, Julia, Rizzotto, Francesco, Costache, Vlad, Schwok, Anny Slama, Gaudu, Philippe, Vidić, Jasmina, "Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3" in Food Chemistry, 433 (2024):137316,
https://doi.org/10.1016/j.foodchem.2023.137316 . .