TY  - JOUR
AU  - Trišić, Dijana
AU  - Ćetenović, Bojana
AU  - Zdravković, Nemanja
AU  - Marković, Tatjana
AU  - Dojčinović, Biljana
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3231
AB  - Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA+) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the antibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA+ had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA+ in 3h and 24h of the incubation period (p < 0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA+. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution.
PB  - Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Antibacterial effects of new endodontic materials based on calcium silicates
T1  - Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata
VL  - 76
IS  - 4
SP  - 365
EP  - 372
DO  - 10.2298/VSP161231130T
ER  - 

@article{
author = "Trišić, Dijana and Ćetenović, Bojana and Zdravković, Nemanja and Marković, Tatjana and Dojčinović, Biljana and Jokanović, Vukoman and Marković, Dejan",
year = "2019",
abstract = "Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA+) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the antibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA+ had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA+ in 3h and 24h of the incubation period (p < 0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA+. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution.",
publisher = "Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Antibacterial effects of new endodontic materials based on calcium silicates, Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata",
volume = "76",
number = "4",
pages = "365-372",
doi = "10.2298/VSP161231130T"
}

Trišić, D., Ćetenović, B., Zdravković, N., Marković, T., Dojčinović, B., Jokanović, V.,& Marković, D.. (2019). Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled
Military Medical Academy, INI., 76(4), 365-372.
https://doi.org/10.2298/VSP161231130T

Trišić D, Ćetenović B, Zdravković N, Marković T, Dojčinović B, Jokanović V, Marković D. Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled. 2019;76(4):365-372.
doi:10.2298/VSP161231130T .

Trišić, Dijana, Ćetenović, Bojana, Zdravković, Nemanja, Marković, Tatjana, Dojčinović, Biljana, Jokanović, Vukoman, Marković, Dejan, "Antibacterial effects of new endodontic materials based on calcium silicates" in Vojnosanitetski pregled, 76, no. 4 (2019):365-372,
https://doi.org/10.2298/VSP161231130T . .

TY  - CONF
AU  - Zizak, I. Besu
AU  - Zizak, B.
AU  - Janović, Barbara
AU  - Vujčić, Miroslava
AU  - Jokanović, Vukoman
AU  - Babić-Stojić, B.
AU  - Čolović, B.
AU  - Vujčić, Zoran
AU  - Stanojković, T.
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4673
AB  - Nanoparticles (NPs) are increasingly used in cancer
therapy as delivery agents and in the diagnosis of malignant diseases
as contrast agents for magnetic resonance imaging (MRI).
The aim of this work was in vitro assessments of Gd-NPs, Fe-
NPs, CoFe-NPs and Graphene Oxide-NPs cytotoxicity and genotoxicity
on some tumour and normal human cell lines.
PB  - Elsevier
C3  - ESMO Open
T1  - PO-410 Cytotoxicity and genotoxicity of new gadolinium, iron oxide, cobalt ferrite and graphene oxide nanoparticles on some tumour cell lines in vitro
VL  - 3
IS  - Suppl 2
SP  - A183
DO  - 10.1136/esmoopen-2018-EACR25.436
ER  - 

@conference{
author = "Zizak, I. Besu and Zizak, B. and Janović, Barbara and Vujčić, Miroslava and Jokanović, Vukoman and Babić-Stojić, B. and Čolović, B. and Vujčić, Zoran and Stanojković, T.",
year = "2018",
abstract = "Nanoparticles (NPs) are increasingly used in cancer
therapy as delivery agents and in the diagnosis of malignant diseases
as contrast agents for magnetic resonance imaging (MRI).
The aim of this work was in vitro assessments of Gd-NPs, Fe-
NPs, CoFe-NPs and Graphene Oxide-NPs cytotoxicity and genotoxicity
on some tumour and normal human cell lines.",
publisher = "Elsevier",
journal = "ESMO Open",
title = "PO-410 Cytotoxicity and genotoxicity of new gadolinium, iron oxide, cobalt ferrite and graphene oxide nanoparticles on some tumour cell lines in vitro",
volume = "3",
number = "Suppl 2",
pages = "A183",
doi = "10.1136/esmoopen-2018-EACR25.436"
}

Zizak, I. B., Zizak, B., Janović, B., Vujčić, M., Jokanović, V., Babić-Stojić, B., Čolović, B., Vujčić, Z.,& Stanojković, T.. (2018). PO-410 Cytotoxicity and genotoxicity of new gadolinium, iron oxide, cobalt ferrite and graphene oxide nanoparticles on some tumour cell lines in vitro. in ESMO Open
Elsevier., 3(Suppl 2), A183.
https://doi.org/10.1136/esmoopen-2018-EACR25.436

Zizak IB, Zizak B, Janović B, Vujčić M, Jokanović V, Babić-Stojić B, Čolović B, Vujčić Z, Stanojković T. PO-410 Cytotoxicity and genotoxicity of new gadolinium, iron oxide, cobalt ferrite and graphene oxide nanoparticles on some tumour cell lines in vitro. in ESMO Open. 2018;3(Suppl 2):A183.
doi:10.1136/esmoopen-2018-EACR25.436 .

Zizak, I. Besu, Zizak, B., Janović, Barbara, Vujčić, Miroslava, Jokanović, Vukoman, Babić-Stojić, B., Čolović, B., Vujčić, Zoran, Stanojković, T., "PO-410 Cytotoxicity and genotoxicity of new gadolinium, iron oxide, cobalt ferrite and graphene oxide nanoparticles on some tumour cell lines in vitro" in ESMO Open, 3, no. Suppl 2 (2018):A183,
https://doi.org/10.1136/esmoopen-2018-EACR25.436 . .

TY  - JOUR
AU  - Cetenovic, Bojana
AU  - Prokic, Bogomir
AU  - Vasilijic, Sasa
AU  - Dojčinović, Biljana
AU  - Magic, Marko
AU  - Jokanovic, Vukoman
AU  - Marković, Dejan
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2186
AB  - Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P  LT .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers
VL  - 43
IS  - 3
SP  - 425
EP  - 432
DO  - 10.1016/j.joen.2016.10.041
ER  - 

@article{
author = "Cetenovic, Bojana and Prokic, Bogomir and Vasilijic, Sasa and Dojčinović, Biljana and Magic, Marko and Jokanovic, Vukoman and Marković, Dejan",
year = "2017",
abstract = "Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P  LT .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers",
volume = "43",
number = "3",
pages = "425-432",
doi = "10.1016/j.joen.2016.10.041"
}

Cetenovic, B., Prokic, B., Vasilijic, S., Dojčinović, B., Magic, M., Jokanovic, V.,& Marković, D.. (2017). Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics
Elsevier Science Inc, New York., 43(3), 425-432.
https://doi.org/10.1016/j.joen.2016.10.041

Cetenovic B, Prokic B, Vasilijic S, Dojčinović B, Magic M, Jokanovic V, Marković D. Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics. 2017;43(3):425-432.
doi:10.1016/j.joen.2016.10.041 .

Cetenovic, Bojana, Prokic, Bogomir, Vasilijic, Sasa, Dojčinović, Biljana, Magic, Marko, Jokanovic, Vukoman, Marković, Dejan, "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers" in Journal of Endodontics, 43, no. 3 (2017):425-432,
https://doi.org/10.1016/j.joen.2016.10.041 . .

TY  - CONF
AU  - Mudrinić, Tihana
AU  - Mojović, Zorica
AU  - Milutinović Nikolić, Aleksandra
AU  - Lončarević, Davor
AU  - Čolović, Božana
AU  - Jokanović, Vukoman
AU  - Jovanović, Dušan
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7017
AB  - Smectites are phyllosilicates of 2:1 type, meaning that octahedral sheet in sandwiched between two tetrahedral sheets. Due to isomorphous substitutions (Al3+ or Fe3+ for tetrahedral Si4+ and Mg2+, Fe2+ or Fe3+ for octahedral Al3+) these sheets have resulting negative charge. Hydrated cations in interlayer region neutralize negative charge. Acid activation of clays consists of mixing clay with acid solution. During acid treatment of clay interlayer cations are replaced by H+ ions what is followed by dissolution of structural cations. The extent of acid activation can be controlled by several factors: the concentration of acid, the ratio between clay and acid solution, temperature and duration of contract between acid and clay. 
In this paper is to investigate acid activation of smectite under very mild conditions. The purpose of this activation is exposing of presence Fe2+/Fe3+ ions to the environment and subsequent investigation of obtained material as an electrode material. The obtained materials were characterized by XRD and FTIR technique. 
According to X-ray diffraction patterns smectite is the main constituent of the investigated clay. The obtained basal spacing d001=1.52 indicates that Ca2+ is the main exchangeable cation, defining raw clay as Ca-bentonite. The XRD analysis confirmed that conditions applied for acid activation were mild enough not to disturb crystal structure of smectite. The lines corresponding to smectite (011 and 060) are present at sample treated with the highest used concentration although in lower intensity indicating that dissolution of clay mineral was partial as intended.  
The three bending vibrations of hydroxyl groups associated with Al3+, Fe2+ and Mg2+ (915 cm-1, 875 cm-1 and 848 cm-1) were present in the FTIR spectra of starting and acid activated samples confirming that acid activation did not completely removed iron ions from smectite framework. 
The acid activated clay samples were tested as electrode materials in acidic electrolyte, 0.1 H2SO4. Aside from current rise at potential range limits due to hydrogen and oxygen evolution, presented CV’s have only one feature at potential around 0.4 V. This peak is ascribed to Fe3+/Fe2+ oxidation/reduction process. The current maximum are obtained for samples treated with 1 M and 4.5 M HCl. The observed potential for oxido-reduction for Fe3+/Fe2+ couple are lower than the standard potential for aqueous Fe3+/Fe2+ couple (+0.77  vs. SHE). The reduction potential is lower for structural Fe3+ than for solution Fe3+. The shift in reduction potential observed for different samples is driven by change of the ratio of Fe(II) to Fe(III) species. The Fe3+/Fe2+ ratio can significantly influence the application of material as catalyst. The acid activation provides an easy way to modify clay to desire
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - Programme and the Book of Abstracts - ROSOV pin 2014, Second Regional Roundtable: Refractory Process and Nanotechnology
T1  - Electrochemical behavior of acid activated clays
SP  - 151
EP  - 152
DO  - 978-86-7306-125-2
ER  - 

@conference{
author = "Mudrinić, Tihana and Mojović, Zorica and Milutinović Nikolić, Aleksandra and Lončarević, Davor and Čolović, Božana and Jokanović, Vukoman and Jovanović, Dušan",
year = "2014",
abstract = "Smectites are phyllosilicates of 2:1 type, meaning that octahedral sheet in sandwiched between two tetrahedral sheets. Due to isomorphous substitutions (Al3+ or Fe3+ for tetrahedral Si4+ and Mg2+, Fe2+ or Fe3+ for octahedral Al3+) these sheets have resulting negative charge. Hydrated cations in interlayer region neutralize negative charge. Acid activation of clays consists of mixing clay with acid solution. During acid treatment of clay interlayer cations are replaced by H+ ions what is followed by dissolution of structural cations. The extent of acid activation can be controlled by several factors: the concentration of acid, the ratio between clay and acid solution, temperature and duration of contract between acid and clay. 
In this paper is to investigate acid activation of smectite under very mild conditions. The purpose of this activation is exposing of presence Fe2+/Fe3+ ions to the environment and subsequent investigation of obtained material as an electrode material. The obtained materials were characterized by XRD and FTIR technique. 
According to X-ray diffraction patterns smectite is the main constituent of the investigated clay. The obtained basal spacing d001=1.52 indicates that Ca2+ is the main exchangeable cation, defining raw clay as Ca-bentonite. The XRD analysis confirmed that conditions applied for acid activation were mild enough not to disturb crystal structure of smectite. The lines corresponding to smectite (011 and 060) are present at sample treated with the highest used concentration although in lower intensity indicating that dissolution of clay mineral was partial as intended.  
The three bending vibrations of hydroxyl groups associated with Al3+, Fe2+ and Mg2+ (915 cm-1, 875 cm-1 and 848 cm-1) were present in the FTIR spectra of starting and acid activated samples confirming that acid activation did not completely removed iron ions from smectite framework. 
The acid activated clay samples were tested as electrode materials in acidic electrolyte, 0.1 H2SO4. Aside from current rise at potential range limits due to hydrogen and oxygen evolution, presented CV’s have only one feature at potential around 0.4 V. This peak is ascribed to Fe3+/Fe2+ oxidation/reduction process. The current maximum are obtained for samples treated with 1 M and 4.5 M HCl. The observed potential for oxido-reduction for Fe3+/Fe2+ couple are lower than the standard potential for aqueous Fe3+/Fe2+ couple (+0.77  vs. SHE). The reduction potential is lower for structural Fe3+ than for solution Fe3+. The shift in reduction potential observed for different samples is driven by change of the ratio of Fe(II) to Fe(III) species. The Fe3+/Fe2+ ratio can significantly influence the application of material as catalyst. The acid activation provides an easy way to modify clay to desire",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "Programme and the Book of Abstracts - ROSOV pin 2014, Second Regional Roundtable: Refractory Process and Nanotechnology",
title = "Electrochemical behavior of acid activated clays",
pages = "151-152",
doi = "978-86-7306-125-2"
}

Mudrinić, T., Mojović, Z., Milutinović Nikolić, A., Lončarević, D., Čolović, B., Jokanović, V.,& Jovanović, D.. (2014). Electrochemical behavior of acid activated clays. in Programme and the Book of Abstracts - ROSOV pin 2014, Second Regional Roundtable: Refractory Process and Nanotechnology
Belgrade : Vinča Institute of Nuclear Sciences., 151-152.
https://doi.org/978-86-7306-125-2

Mudrinić T, Mojović Z, Milutinović Nikolić A, Lončarević D, Čolović B, Jokanović V, Jovanović D. Electrochemical behavior of acid activated clays. in Programme and the Book of Abstracts - ROSOV pin 2014, Second Regional Roundtable: Refractory Process and Nanotechnology. 2014;:151-152.
doi:978-86-7306-125-2 .

Mudrinić, Tihana, Mojović, Zorica, Milutinović Nikolić, Aleksandra, Lončarević, Davor, Čolović, Božana, Jokanović, Vukoman, Jovanović, Dušan, "Electrochemical behavior of acid activated clays" in Programme and the Book of Abstracts - ROSOV pin 2014, Second Regional Roundtable: Refractory Process and Nanotechnology (2014):151-152,
https://doi.org/978-86-7306-125-2 . .