TY  - JOUR
AU  - Radović, Jelena
AU  - Popović, Dragana
AU  - Ćurčić, Tatjana
AU  - Veličković, Luka
AU  - Lević, Steva
AU  - Pavlović, Vladimir
AU  - Minić, Simeon
AU  - Nikolić, Milan
AU  - Gligorijević, Nikola
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7596
AB  - Mercury emissions represent a significant risk to the environment and human health. Mercury is persistent and
can circulate in the environment for thousands of years, which is why treating this toxic metal is important.
Chitosan polymer is easily obtainable, and it has good mercury adsorption characteristics. This study aimed to
improve its capabilities to absorb mercury by immobilizing phycobiliproteins (PBPs) onto the surface of chitosan
beads (chitosan–PBPs). Phycobiliproteins, light-harvesting proteins from algae and cyanobacteria, have several
industrially essential applications. These proteins can bind heavy metals with high affinities. Protein extracts
obtained from both Arthrospira platensis, with C-phycocyanin (C-PC) as the primary protein, and Porphyra
yezoensis, with R-phycocyanin (R-PC) and R-phycoerythrin (R-PE) as the dominant PBPs, were covalently
immobilized onto chitosan beads. Beads with immobilized PBPs were characterized by scanning electron microscopy
and Fourier transform infrared spectroscopy. Binding analysis showed that, on average, each chitosan
bead weighed 20 mg and immobilized 63.54 μg of PBPs from Spirulina and 44.12 μg of PBPs from Porphyra.
Immobilized proteins were still in their native state, with no visible color change months after immobilization.
Chitosan–PBPs and chitosan alone were tested for mercury adsorption at pH 4 and pH 7 by atomic absorption
spectroscopy. The tested concentration range of mercury was from 1 to 70 ppm. Affinity, calculated using
Henry’s binding isotherm, of chitosan–PBPs for mercury was twice as much higher at both pH values than
chitosan alone. Furthermore, chitosan-PBP beads were able to absorb more mercury than chitosan alone. These
results showed that the covalent immobilization of PBPs onto chitosan improves its mercury adsorption characteristics
and creates a more efficient eco-friendly adsorbent to potentially remove mercury ions in the tested
concentration range from polluted waters.
PB  - Elsevier
T2  - Algal Research
T1  - Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads
VL  - 80
SP  - 103543
DO  - 10.1016/j.algal.2024.103543
ER  - 

@article{
author = "Radović, Jelena and Popović, Dragana and Ćurčić, Tatjana and Veličković, Luka and Lević, Steva and Pavlović, Vladimir and Minić, Simeon and Nikolić, Milan and Gligorijević, Nikola",
year = "2024",
abstract = "Mercury emissions represent a significant risk to the environment and human health. Mercury is persistent and
can circulate in the environment for thousands of years, which is why treating this toxic metal is important.
Chitosan polymer is easily obtainable, and it has good mercury adsorption characteristics. This study aimed to
improve its capabilities to absorb mercury by immobilizing phycobiliproteins (PBPs) onto the surface of chitosan
beads (chitosan–PBPs). Phycobiliproteins, light-harvesting proteins from algae and cyanobacteria, have several
industrially essential applications. These proteins can bind heavy metals with high affinities. Protein extracts
obtained from both Arthrospira platensis, with C-phycocyanin (C-PC) as the primary protein, and Porphyra
yezoensis, with R-phycocyanin (R-PC) and R-phycoerythrin (R-PE) as the dominant PBPs, were covalently
immobilized onto chitosan beads. Beads with immobilized PBPs were characterized by scanning electron microscopy
and Fourier transform infrared spectroscopy. Binding analysis showed that, on average, each chitosan
bead weighed 20 mg and immobilized 63.54 μg of PBPs from Spirulina and 44.12 μg of PBPs from Porphyra.
Immobilized proteins were still in their native state, with no visible color change months after immobilization.
Chitosan–PBPs and chitosan alone were tested for mercury adsorption at pH 4 and pH 7 by atomic absorption
spectroscopy. The tested concentration range of mercury was from 1 to 70 ppm. Affinity, calculated using
Henry’s binding isotherm, of chitosan–PBPs for mercury was twice as much higher at both pH values than
chitosan alone. Furthermore, chitosan-PBP beads were able to absorb more mercury than chitosan alone. These
results showed that the covalent immobilization of PBPs onto chitosan improves its mercury adsorption characteristics
and creates a more efficient eco-friendly adsorbent to potentially remove mercury ions in the tested
concentration range from polluted waters.",
publisher = "Elsevier",
journal = "Algal Research",
title = "Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads",
volume = "80",
pages = "103543",
doi = "10.1016/j.algal.2024.103543"
}

Radović, J., Popović, D., Ćurčić, T., Veličković, L., Lević, S., Pavlović, V., Minić, S., Nikolić, M.,& Gligorijević, N.. (2024). Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads. in Algal Research
Elsevier., 80, 103543.
https://doi.org/10.1016/j.algal.2024.103543

Radović J, Popović D, Ćurčić T, Veličković L, Lević S, Pavlović V, Minić S, Nikolić M, Gligorijević N. Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads. in Algal Research. 2024;80:103543.
doi:10.1016/j.algal.2024.103543 .

Radović, Jelena, Popović, Dragana, Ćurčić, Tatjana, Veličković, Luka, Lević, Steva, Pavlović, Vladimir, Minić, Simeon, Nikolić, Milan, Gligorijević, Nikola, "Probing the potential of mercury removal by covalently immobilized phycobiliproteins onto the surface of chitosan beads" in Algal Research, 80 (2024):103543,
https://doi.org/10.1016/j.algal.2024.103543 . .

TY  - CONF
AU  - Radović, Jelena
AU  - Popović, Dragana
AU  - Ćurčić, Tatjana
AU  - Nikolić, Milan
AU  - Minić, Simeon
AU  - Gligorijević, Nikola
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6666
AB  - This study aimed to improve chitosan polymer's capabilities to absorb mercury by
immobilising phycobiliproteins (PBPs) onto the surface of chitosan beads (chitosan–
PBPs). Phycobiliproteins, light-harvesting proteins from algae and cyanobacteria, have
several industrially essential applications. These proteins can bind heavy metals with high
affinities. Protein extracts obtained from both Arthrospira platensis, with C-phycocyanin
as the dominant phycobiliprotein and Neoporphyra haitanensis, with R-phycocyanin and
R-phycoerythrin as the dominant PBPs, were covalently immobilised onto chitosan beads.
Binding analysis showed that, on average, 54 μg of PBPs were immobilised per bead.
Immobilised proteins were still in their native state, with no visible colour change after
immobilisation. Chitosan–PBPs and chitosan alone were tested for mercury adsorption at
pH 4 and pH 7 by atomic absorption spectroscopy. The tested concentration range of
mercury was from 1 to 70 ppm. Affinity, calculated using Henry's binding isotherm, of
chitosan–PBPs for mercury was higher at both pH values than chitosan alone.
Furthermore, chitosan–PBPs beads were able to absorb significantly more mercury than
chitosan alone. These results show that the covalent immobilisation of PBPs onto chitosan
improves its mercury adsorption characteristics and creates a more efficient eco-friendly
adsorbent for removing mercury ions in the tested concentration range.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia
T1  - Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal
SP  - 116
EP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_cer_6666
ER  - 

@conference{
author = "Radović, Jelena and Popović, Dragana and Ćurčić, Tatjana and Nikolić, Milan and Minić, Simeon and Gligorijević, Nikola",
year = "2023",
abstract = "This study aimed to improve chitosan polymer's capabilities to absorb mercury by
immobilising phycobiliproteins (PBPs) onto the surface of chitosan beads (chitosan–
PBPs). Phycobiliproteins, light-harvesting proteins from algae and cyanobacteria, have
several industrially essential applications. These proteins can bind heavy metals with high
affinities. Protein extracts obtained from both Arthrospira platensis, with C-phycocyanin
as the dominant phycobiliprotein and Neoporphyra haitanensis, with R-phycocyanin and
R-phycoerythrin as the dominant PBPs, were covalently immobilised onto chitosan beads.
Binding analysis showed that, on average, 54 μg of PBPs were immobilised per bead.
Immobilised proteins were still in their native state, with no visible colour change after
immobilisation. Chitosan–PBPs and chitosan alone were tested for mercury adsorption at
pH 4 and pH 7 by atomic absorption spectroscopy. The tested concentration range of
mercury was from 1 to 70 ppm. Affinity, calculated using Henry's binding isotherm, of
chitosan–PBPs for mercury was higher at both pH values than chitosan alone.
Furthermore, chitosan–PBPs beads were able to absorb significantly more mercury than
chitosan alone. These results show that the covalent immobilisation of PBPs onto chitosan
improves its mercury adsorption characteristics and creates a more efficient eco-friendly
adsorbent for removing mercury ions in the tested concentration range.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia",
title = "Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal",
pages = "116-116",
url = "https://hdl.handle.net/21.15107/rcub_cer_6666"
}

Radović, J., Popović, D., Ćurčić, T., Nikolić, M., Minić, S.,& Gligorijević, N.. (2023). Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia
Serbian Biochemical Society., 116-116.
https://hdl.handle.net/21.15107/rcub_cer_6666

Radović J, Popović D, Ćurčić T, Nikolić M, Minić S, Gligorijević N. Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia. 2023;:116-116.
https://hdl.handle.net/21.15107/rcub_cer_6666 .

Radović, Jelena, Popović, Dragana, Ćurčić, Tatjana, Nikolić, Milan, Minić, Simeon, Gligorijević, Nikola, "Applying immobilised phycobiliproteins onto chitosan for efficient mercury removal" in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia (2023):116-116,
https://hdl.handle.net/21.15107/rcub_cer_6666 .