TY  - JOUR
AU  - Lović, Jelena
AU  - Stevanović, Sanja
AU  - Anđelković, Boban D.
AU  - Petrovic, S.
AU  - Vukovic, D.
AU  - Prlainović, Nevena Ž.
AU  - Mijin, Dušan
AU  - Nikolić, Nebojša D.
AU  - Avramov Ivić, Milka
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2364
AB  - Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.
PB  - Esg, Belgrade
T2  - International Journal of Electrochemical Science
T1  - Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode
VL  - 13
IS  - 12
SP  - 12340
EP  - 12348
DO  - 10.20964/2018.12.59
ER  - 

@article{
author = "Lović, Jelena and Stevanović, Sanja and Anđelković, Boban D. and Petrovic, S. and Vukovic, D. and Prlainović, Nevena Ž. and Mijin, Dušan and Nikolić, Nebojša D. and Avramov Ivić, Milka",
year = "2018",
abstract = "Glucose biosensor containing cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is constructed and already electrochemically tested. Now, the electrochemical behavior of biosensor in human serum is further investigated and supported by morphological characterization of layers for the first time. The morphology and microstructure of layers was examined by Fourier transformed infra red spectroscopy (FTIR), atomic force (AFM) and optical microscopy (OM). The electrochemical indication that the Cys-GA-GOx film on Au surface is not compact and that there were some bare regions which remain catalytically active is supported by AFM and OM results. The construction and the nature of bonding of Au-Cys-GA-GOx biosensor layers is confirmed by the FTIR study.",
publisher = "Esg, Belgrade",
journal = "International Journal of Electrochemical Science",
title = "Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode",
volume = "13",
number = "12",
pages = "12340-12348",
doi = "10.20964/2018.12.59"
}

Lović, J., Stevanović, S., Anđelković, B. D., Petrovic, S., Vukovic, D., Prlainović, N. Ž., Mijin, D., Nikolić, N. D.,& Avramov Ivić, M.. (2018). Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode. in International Journal of Electrochemical Science
Esg, Belgrade., 13(12), 12340-12348.
https://doi.org/10.20964/2018.12.59

Lović J, Stevanović S, Anđelković BD, Petrovic S, Vukovic D, Prlainović NŽ, Mijin D, Nikolić ND, Avramov Ivić M. Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode. in International Journal of Electrochemical Science. 2018;13(12):12340-12348.
doi:10.20964/2018.12.59 .

Lović, Jelena, Stevanović, Sanja, Anđelković, Boban D., Petrovic, S., Vukovic, D., Prlainović, Nevena Ž., Mijin, Dušan, Nikolić, Nebojša D., Avramov Ivić, Milka, "Electrochemical glucose biosensor with the characterization of surface morphology and content of glucose oxidase-glutaraldehyde-cysteine layers on gold electrode" in International Journal of Electrochemical Science, 13, no. 12 (2018):12340-12348,
https://doi.org/10.20964/2018.12.59 . .

TY  - JOUR
AU  - Lović, Jelena
AU  - Stevanović, Sanja
AU  - Nikolić, Nebojša D.
AU  - Petrovic, S.
AU  - Vukovic, D.
AU  - Prlainović, Nevena Ž.
AU  - Mijin, Dušan
AU  - Avramov Ivić, Milka
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2141
AB  - The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.
PB  - Esg, Belgrade
T2  - International Journal of Electrochemical Science
T1  - Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode
VL  - 12
IS  - 7
SP  - 5806
EP  - 5817
DO  - 10.20964/2017.07.65
ER  - 

@article{
author = "Lović, Jelena and Stevanović, Sanja and Nikolić, Nebojša D. and Petrovic, S. and Vukovic, D. and Prlainović, Nevena Ž. and Mijin, Dušan and Avramov Ivić, Milka",
year = "2017",
abstract = "The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k(0) = 20.4 s(-1), high affinity for glucose with the apparent MichaelisMenten constant K-M(app) = 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.",
publisher = "Esg, Belgrade",
journal = "International Journal of Electrochemical Science",
title = "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode",
volume = "12",
number = "7",
pages = "5806-5817",
doi = "10.20964/2017.07.65"
}

Lović, J., Stevanović, S., Nikolić, N. D., Petrovic, S., Vukovic, D., Prlainović, N. Ž., Mijin, D.,& Avramov Ivić, M.. (2017). Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. in International Journal of Electrochemical Science
Esg, Belgrade., 12(7), 5806-5817.
https://doi.org/10.20964/2017.07.65

Lović J, Stevanović S, Nikolić ND, Petrovic S, Vukovic D, Prlainović NŽ, Mijin D, Avramov Ivić M. Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode. in International Journal of Electrochemical Science. 2017;12(7):5806-5817.
doi:10.20964/2017.07.65 .

Lović, Jelena, Stevanović, Sanja, Nikolić, Nebojša D., Petrovic, S., Vukovic, D., Prlainović, Nevena Ž., Mijin, Dušan, Avramov Ivić, Milka, "Glucose Sensing Using Glucose Oxidase-GlutaraldehydeCysteine Modified Gold Electrode" in International Journal of Electrochemical Science, 12, no. 7 (2017):5806-5817,
https://doi.org/10.20964/2017.07.65 . .