TY  - JOUR
AU  - Jauković, Valentina
AU  - Krajišnik, Danina R.
AU  - Daković, Aleksandra S.
AU  - Damjanović, Ana B.
AU  - Krstić, Jugoslav
AU  - Stojanović, Jovica
AU  - Čalija, Bojan
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4487
AB  - The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release
VL  - 123
SP  - 112029
DO  - 10.1016/j.msec.2021.112029
ER  - 

@article{
author = "Jauković, Valentina and Krajišnik, Danina R. and Daković, Aleksandra S. and Damjanović, Ana B. and Krstić, Jugoslav and Stojanović, Jovica and Čalija, Bojan",
year = "2021",
abstract = "The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release",
volume = "123",
pages = "112029",
doi = "10.1016/j.msec.2021.112029"
}

Jauković, V., Krajišnik, D. R., Daković, A. S., Damjanović, A. B., Krstić, J., Stojanović, J.,& Čalija, B.. (2021). Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C
Elsevier., 123, 112029.
https://doi.org/10.1016/j.msec.2021.112029

Jauković V, Krajišnik DR, Daković AS, Damjanović AB, Krstić J, Stojanović J, Čalija B. Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C. 2021;123:112029.
doi:10.1016/j.msec.2021.112029 .

Jauković, Valentina, Krajišnik, Danina R., Daković, Aleksandra S., Damjanović, Ana B., Krstić, Jugoslav, Stojanović, Jovica, Čalija, Bojan, "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release" in Materials Science and Engineering C, 123 (2021):112029,
https://doi.org/10.1016/j.msec.2021.112029 . .

TY  - JOUR
AU  - Cvetković, Mirjana
AU  - Damjanović, Ana
AU  - Stanojković, Tatjana
AU  - Đorđević, Iris
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
AU  - Gođevac, Dejan
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3054
AB  - Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using dry-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIR based metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line
(A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed.
PB  - Elsevier
T2  - Talanta
T1  - Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus
VL  - 206
SP  - 120248
DO  - 10.1016/j.talanta.2019.120248
ER  - 

@article{
author = "Cvetković, Mirjana and Damjanović, Ana and Stanojković, Tatjana and Đorđević, Iris and Tešević, Vele and Milosavljević, Slobodan and Gođevac, Dejan",
year = "2020",
abstract = "Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using dry-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIR based metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line
(A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed.",
publisher = "Elsevier",
journal = "Talanta",
title = "Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus",
volume = "206",
pages = "120248",
doi = "10.1016/j.talanta.2019.120248"
}

Cvetković, M., Damjanović, A., Stanojković, T., Đorđević, I., Tešević, V., Milosavljević, S.,& Gođevac, D.. (2020). Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus. in Talanta
Elsevier., 206, 120248.
https://doi.org/10.1016/j.talanta.2019.120248

Cvetković M, Damjanović A, Stanojković T, Đorđević I, Tešević V, Milosavljević S, Gođevac D. Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus. in Talanta. 2020;206:120248.
doi:10.1016/j.talanta.2019.120248 .

Cvetković, Mirjana, Damjanović, Ana, Stanojković, Tatjana, Đorđević, Iris, Tešević, Vele, Milosavljević, Slobodan, Gođevac, Dejan, "Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus" in Talanta, 206 (2020):120248,
https://doi.org/10.1016/j.talanta.2019.120248 . .

TY  - JOUR
AU  - Sović, Irena
AU  - Cindrić, Maja
AU  - Perin, Nataša
AU  - Boček, Ida
AU  - Novaković, Irena
AU  - Damjanović, Ana
AU  - Stanojković, Tatjana
AU  - Zlatović, Mario
AU  - Hranjec, Marijana
AU  - Bertoša, Branimir
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3851
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3416
AB  - This paper discusses antioxidative and biological activities of 25 novel amidino substituted benzamides with a variety of heteroaromatic nuclei attached to the benzamide moiety and with a variable number of methoxy or hydroxy substituents. Targeted compounds, bearing either amidino or 2-imidazolinyl substituent, were obtained in the Pinner reaction from cyano precursors. 3D-QSAR models were generated to predict antioxidative activity of the 25 novel aromatic and heteroaromatic benzamide derivatives. The compounds were tested for antioxidative activity using in vitro spectrophotometric assays. Direct validation of 3D-QSAR approach for predicting activities of novel benzamide derivatives was carried out by comparing experimental and computationally predicted antioxidative activity. Experimentally determined activities for all novel compounds were found to be within a standard deviation of error of the models. Following this, structure–activity relationships among the synthesized compounds are discussed. Furthermore, antiproliferative activity in vitro against HeLa cells as well as antibacterial and antifungal activity was tested to confirm the other biological activities of the prepared compounds.
PB  - American Chemical Society (ACS)
T2  - Chemical Research in Toxicology
T1  - Biological Potential of Novel Methoxy and Hydroxy Substituted Heteroaromatic Amides Designed as Promising Antioxidative Agents: Synthesis, 3D-QSAR Analysis, and Biological Activity.
VL  - 32
IS  - 9
SP  - 1880
EP  - 1892
DO  - 10.1021/acs.chemrestox.9b00256
ER  - 

@article{
author = "Sović, Irena and Cindrić, Maja and Perin, Nataša and Boček, Ida and Novaković, Irena and Damjanović, Ana and Stanojković, Tatjana and Zlatović, Mario and Hranjec, Marijana and Bertoša, Branimir",
year = "2019",
abstract = "This paper discusses antioxidative and biological activities of 25 novel amidino substituted benzamides with a variety of heteroaromatic nuclei attached to the benzamide moiety and with a variable number of methoxy or hydroxy substituents. Targeted compounds, bearing either amidino or 2-imidazolinyl substituent, were obtained in the Pinner reaction from cyano precursors. 3D-QSAR models were generated to predict antioxidative activity of the 25 novel aromatic and heteroaromatic benzamide derivatives. The compounds were tested for antioxidative activity using in vitro spectrophotometric assays. Direct validation of 3D-QSAR approach for predicting activities of novel benzamide derivatives was carried out by comparing experimental and computationally predicted antioxidative activity. Experimentally determined activities for all novel compounds were found to be within a standard deviation of error of the models. Following this, structure–activity relationships among the synthesized compounds are discussed. Furthermore, antiproliferative activity in vitro against HeLa cells as well as antibacterial and antifungal activity was tested to confirm the other biological activities of the prepared compounds.",
publisher = "American Chemical Society (ACS)",
journal = "Chemical Research in Toxicology",
title = "Biological Potential of Novel Methoxy and Hydroxy Substituted Heteroaromatic Amides Designed as Promising Antioxidative Agents: Synthesis, 3D-QSAR Analysis, and Biological Activity.",
volume = "32",
number = "9",
pages = "1880-1892",
doi = "10.1021/acs.chemrestox.9b00256"
}

Sović, I., Cindrić, M., Perin, N., Boček, I., Novaković, I., Damjanović, A., Stanojković, T., Zlatović, M., Hranjec, M.,& Bertoša, B.. (2019). Biological Potential of Novel Methoxy and Hydroxy Substituted Heteroaromatic Amides Designed as Promising Antioxidative Agents: Synthesis, 3D-QSAR Analysis, and Biological Activity.. in Chemical Research in Toxicology
American Chemical Society (ACS)., 32(9), 1880-1892.
https://doi.org/10.1021/acs.chemrestox.9b00256

Sović I, Cindrić M, Perin N, Boček I, Novaković I, Damjanović A, Stanojković T, Zlatović M, Hranjec M, Bertoša B. Biological Potential of Novel Methoxy and Hydroxy Substituted Heteroaromatic Amides Designed as Promising Antioxidative Agents: Synthesis, 3D-QSAR Analysis, and Biological Activity.. in Chemical Research in Toxicology. 2019;32(9):1880-1892.
doi:10.1021/acs.chemrestox.9b00256 .

Sović, Irena, Cindrić, Maja, Perin, Nataša, Boček, Ida, Novaković, Irena, Damjanović, Ana, Stanojković, Tatjana, Zlatović, Mario, Hranjec, Marijana, Bertoša, Branimir, "Biological Potential of Novel Methoxy and Hydroxy Substituted Heteroaromatic Amides Designed as Promising Antioxidative Agents: Synthesis, 3D-QSAR Analysis, and Biological Activity." in Chemical Research in Toxicology, 32, no. 9 (2019):1880-1892,
https://doi.org/10.1021/acs.chemrestox.9b00256 . .

TY  - JOUR
AU  - Sović, Irena
AU  - Cindrić, Maja
AU  - Perin, Nataša
AU  - Boček, Ida
AU  - Novaković, Irena
AU  - Damjanović, Ana
AU  - Stanojković, Tatjana
AU  - Zlatović, Mario
AU  - Hranjec, Marijana
AU  - Bertoša, Branimir
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3464
AB  - This paper discusses antioxidative and biological activities of 25 novel amidino substituted benzamides with a variety of heteroaromatic nuclei attached to the benzamide moiety and with a variable number of methoxy or hydroxy substituents. Targeted compounds, bearing either amidino or 2-imidazolinyl substituent, were obtained in the Pinner reaction from cyano precursors. 3D-QSAR models were generated to predict antioxidative activity of the 25 novel aromatic and heteroaromatic benzamide derivatives. The compounds were tested for antioxidative activity using in vitro spectrophotometric assays. Direct validation of 3D-QSAR approach for predicting activities of novel benzamide derivatives was carried out by comparing experimental and computationally predicted antioxidative activity. Experimentally determined activities for all novel compounds were found to be within a standard deviation of error of the models. Following this, structure–activity relationships among the synthesized compounds are discussed. Furthermore, antiproliferative activity in vitro against HeLa cells as well as antibacterial and antifungal activity was tested to confirm the other biological activities of the prepared compounds.
PB  - American Chemical Society (ACS)
T2  - Chemical Research in Toxicology
T1  - Biological Potential of Novel Methoxy and Hydroxy Substituted Heteroaromatic Amides Designed as Promising Antioxidative Agents: Synthesis, 3D-QSAR Analysis, and Biological Activity.
VL  - 32
IS  - 9
SP  - 1880
EP  - 1892
DO  - 10.1021/acs.chemrestox.9b00256
ER  - 

@article{
author = "Sović, Irena and Cindrić, Maja and Perin, Nataša and Boček, Ida and Novaković, Irena and Damjanović, Ana and Stanojković, Tatjana and Zlatović, Mario and Hranjec, Marijana and Bertoša, Branimir",
year = "2019",
abstract = "This paper discusses antioxidative and biological activities of 25 novel amidino substituted benzamides with a variety of heteroaromatic nuclei attached to the benzamide moiety and with a variable number of methoxy or hydroxy substituents. Targeted compounds, bearing either amidino or 2-imidazolinyl substituent, were obtained in the Pinner reaction from cyano precursors. 3D-QSAR models were generated to predict antioxidative activity of the 25 novel aromatic and heteroaromatic benzamide derivatives. The compounds were tested for antioxidative activity using in vitro spectrophotometric assays. Direct validation of 3D-QSAR approach for predicting activities of novel benzamide derivatives was carried out by comparing experimental and computationally predicted antioxidative activity. Experimentally determined activities for all novel compounds were found to be within a standard deviation of error of the models. Following this, structure–activity relationships among the synthesized compounds are discussed. Furthermore, antiproliferative activity in vitro against HeLa cells as well as antibacterial and antifungal activity was tested to confirm the other biological activities of the prepared compounds.",
publisher = "American Chemical Society (ACS)",
journal = "Chemical Research in Toxicology",
title = "Biological Potential of Novel Methoxy and Hydroxy Substituted Heteroaromatic Amides Designed as Promising Antioxidative Agents: Synthesis, 3D-QSAR Analysis, and Biological Activity.",
volume = "32",
number = "9",
pages = "1880-1892",
doi = "10.1021/acs.chemrestox.9b00256"
}

Sović, I., Cindrić, M., Perin, N., Boček, I., Novaković, I., Damjanović, A., Stanojković, T., Zlatović, M., Hranjec, M.,& Bertoša, B.. (2019). Biological Potential of Novel Methoxy and Hydroxy Substituted Heteroaromatic Amides Designed as Promising Antioxidative Agents: Synthesis, 3D-QSAR Analysis, and Biological Activity.. in Chemical Research in Toxicology
American Chemical Society (ACS)., 32(9), 1880-1892.
https://doi.org/10.1021/acs.chemrestox.9b00256

Sović I, Cindrić M, Perin N, Boček I, Novaković I, Damjanović A, Stanojković T, Zlatović M, Hranjec M, Bertoša B. Biological Potential of Novel Methoxy and Hydroxy Substituted Heteroaromatic Amides Designed as Promising Antioxidative Agents: Synthesis, 3D-QSAR Analysis, and Biological Activity.. in Chemical Research in Toxicology. 2019;32(9):1880-1892.
doi:10.1021/acs.chemrestox.9b00256 .

Sović, Irena, Cindrić, Maja, Perin, Nataša, Boček, Ida, Novaković, Irena, Damjanović, Ana, Stanojković, Tatjana, Zlatović, Mario, Hranjec, Marijana, Bertoša, Branimir, "Biological Potential of Novel Methoxy and Hydroxy Substituted Heteroaromatic Amides Designed as Promising Antioxidative Agents: Synthesis, 3D-QSAR Analysis, and Biological Activity." in Chemical Research in Toxicology, 32, no. 9 (2019):1880-1892,
https://doi.org/10.1021/acs.chemrestox.9b00256 . .