TY  - JOUR
AU  - Stojković, Pavle
AU  - Kostić, Ana
AU  - Lupšić, Ema
AU  - Terzić-Jovanović, Nataša
AU  - Novaković, Miroslav
AU  - Nedialkov, Paraskev
AU  - Trendafilova, Antoaneta
AU  - Pešić, Milica
AU  - Opsenica, Igor
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7183
AB  - The synthesis of 24 hybrid molecules, consisting of naturally occurring sclareol (SCL) and synthetic 1,2,4-triazolo[1,5-a]pyrimidines (TPs), is described. New compounds were designed with the aim of improving the cytotoxic properties, activity, and selectivity of the parent compounds. Six analogs (12a-f) contained 4-benzylpiperazine linkage, while 4-benzyldiamine linkage was present in eighteen derivatives (12g-r and 13a-f). Hybrids 13a-f consist of two TP units. After purification, all hybrids (12a-r and 13a-f), as well as their precursors (9a-e and 11a-c), were tested on human glioblastoma U87 cells. More than half of the tested synthesized molecules, 16 out of 31, caused a significant reduction of U87 cell viability (more than 75% reduction) at 30 µM. The concentration-dependent cytotoxicity of these 16 compounds was also examined on U87 cells, corresponding multidrug-resistant (MDR) U87-TxR cells with increased P-glycoprotein (P-gp) expression and activity, and normal lung fibroblasts MRC-5. Importantly, 12l and 12r were active in the nanomolar range, while seven compounds (11b, 11c, 12i, 12l, 12n, 12q, and 12r) were more selective towards glioblastoma cells than SCL. All compounds except 12r evaded MDR, showing even better cytotoxicity in U87-TxR cells. In particular, 11c, 12a, 12g, 12j, 12k, 12m, 12n, and SCL showed collateral sensitivity. Hybrid compounds 12l, 12q, and 12r decreased P-gp activity to the same extent as a well-known P-gp inhibitor - tariquidar (TQ). Hybrid compound 12l and its precursor 11c affected different cellular processes including the cell cycle, cell death, and mitochondrial membrane potential, and changed the levels of reactive oxygen and nitrogen species (ROS/RNS) in glioblastoma cells. Collateral sensitivity towards MDR glioblastoma cells was caused by the modulation of oxidative stress accompanied by inhibition of mitochondria.
PB  - Elsevier
T2  - Bioorganic Chemistry
T1  - Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells
VL  - 138
SP  - 106605
DO  - 10.1016/j.bioorg.2023.106605
ER  - 

@article{
author = "Stojković, Pavle and Kostić, Ana and Lupšić, Ema and Terzić-Jovanović, Nataša and Novaković, Miroslav and Nedialkov, Paraskev and Trendafilova, Antoaneta and Pešić, Milica and Opsenica, Igor",
year = "2023",
abstract = "The synthesis of 24 hybrid molecules, consisting of naturally occurring sclareol (SCL) and synthetic 1,2,4-triazolo[1,5-a]pyrimidines (TPs), is described. New compounds were designed with the aim of improving the cytotoxic properties, activity, and selectivity of the parent compounds. Six analogs (12a-f) contained 4-benzylpiperazine linkage, while 4-benzyldiamine linkage was present in eighteen derivatives (12g-r and 13a-f). Hybrids 13a-f consist of two TP units. After purification, all hybrids (12a-r and 13a-f), as well as their precursors (9a-e and 11a-c), were tested on human glioblastoma U87 cells. More than half of the tested synthesized molecules, 16 out of 31, caused a significant reduction of U87 cell viability (more than 75% reduction) at 30 µM. The concentration-dependent cytotoxicity of these 16 compounds was also examined on U87 cells, corresponding multidrug-resistant (MDR) U87-TxR cells with increased P-glycoprotein (P-gp) expression and activity, and normal lung fibroblasts MRC-5. Importantly, 12l and 12r were active in the nanomolar range, while seven compounds (11b, 11c, 12i, 12l, 12n, 12q, and 12r) were more selective towards glioblastoma cells than SCL. All compounds except 12r evaded MDR, showing even better cytotoxicity in U87-TxR cells. In particular, 11c, 12a, 12g, 12j, 12k, 12m, 12n, and SCL showed collateral sensitivity. Hybrid compounds 12l, 12q, and 12r decreased P-gp activity to the same extent as a well-known P-gp inhibitor - tariquidar (TQ). Hybrid compound 12l and its precursor 11c affected different cellular processes including the cell cycle, cell death, and mitochondrial membrane potential, and changed the levels of reactive oxygen and nitrogen species (ROS/RNS) in glioblastoma cells. Collateral sensitivity towards MDR glioblastoma cells was caused by the modulation of oxidative stress accompanied by inhibition of mitochondria.",
publisher = "Elsevier",
journal = "Bioorganic Chemistry",
title = "Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells",
volume = "138",
pages = "106605",
doi = "10.1016/j.bioorg.2023.106605"
}

Stojković, P., Kostić, A., Lupšić, E., Terzić-Jovanović, N., Novaković, M., Nedialkov, P., Trendafilova, A., Pešić, M.,& Opsenica, I.. (2023). Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells. in Bioorganic Chemistry
Elsevier., 138, 106605.
https://doi.org/10.1016/j.bioorg.2023.106605

Stojković P, Kostić A, Lupšić E, Terzić-Jovanović N, Novaković M, Nedialkov P, Trendafilova A, Pešić M, Opsenica I. Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells. in Bioorganic Chemistry. 2023;138:106605.
doi:10.1016/j.bioorg.2023.106605 .

Stojković, Pavle, Kostić, Ana, Lupšić, Ema, Terzić-Jovanović, Nataša, Novaković, Miroslav, Nedialkov, Paraskev, Trendafilova, Antoaneta, Pešić, Milica, Opsenica, Igor, "Novel hybrids of sclareol and 1,2,4-triazolo[1,5-a]pyrimidine show collateral sensitivity in multidrug-resistant glioblastoma cells" in Bioorganic Chemistry, 138 (2023):106605,
https://doi.org/10.1016/j.bioorg.2023.106605 . .

TY  - JOUR
AU  - Koračak, Ljiljana
AU  - Lupšić, Ema
AU  - Terzić-Jovanović, Nataša
AU  - Jovanović, Mirna
AU  - Novaković, Miroslav
AU  - Nedialkov, Paraskev
AU  - Trendafilova, Antoaneta
AU  - Zlatović, Mario
AU  - Pešić, Milica
AU  - Opsenica, Igor
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7172
AB  - The synthesis of 17 hybrid molecules, consisting of artesunate, a derivative of naturally occurring artemisinin, and synthetic 4-aryl-2-aminopyrimidines, is described. New compounds were designed to improve the parent compounds' cytotoxic properties, activity, and selectivity. The synthesized hybrid molecules (15a–f with ethylenediamine linker and 16a–k with piperazine linker), as well as their precursors – pyrimidine derivatives (13a–f and 14a–k), artemisinin, and artesunate, were tested on sensitive and multidrug-resistant (MDR) human non-small cell lung carcinoma (NSCLC) cells. All hybrid compounds with piperazine linker 16a–k were selective toward NSCLC cells and displayed IC50 values below 5 μM. Although they showed similar anticancer potency as artesunate, their selectivity against cancer cells was considerably improved. Importantly, 16h–k hybrid compounds were able to evade MDR phenotype, inhibit P-glycoprotein (P-gp) activity, and increase the sensitivity of MDR NSCLC cells to doxorubicin (DOX). The inhibition of P-gp activity induced by 16h–j was stronger than the one obtained with artesunate. Among these four hybrid compounds, 16k was the most potent anticancer agent with similar IC50 values of around 1.5 μM (for comparison – over 3.1 μM for artesunate) in sensitive and MDR NSCLC cells.
PB  - Royal Society of Chemistry
T2  - New Journal of Chemistry
T1  - Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells
VL  - 47
IS  - 14
SP  - 6844
EP  - 6855
DO  - 10.1039/D3NJ00427A
ER  - 

@article{
author = "Koračak, Ljiljana and Lupšić, Ema and Terzić-Jovanović, Nataša and Jovanović, Mirna and Novaković, Miroslav and Nedialkov, Paraskev and Trendafilova, Antoaneta and Zlatović, Mario and Pešić, Milica and Opsenica, Igor",
year = "2023",
abstract = "The synthesis of 17 hybrid molecules, consisting of artesunate, a derivative of naturally occurring artemisinin, and synthetic 4-aryl-2-aminopyrimidines, is described. New compounds were designed to improve the parent compounds' cytotoxic properties, activity, and selectivity. The synthesized hybrid molecules (15a–f with ethylenediamine linker and 16a–k with piperazine linker), as well as their precursors – pyrimidine derivatives (13a–f and 14a–k), artemisinin, and artesunate, were tested on sensitive and multidrug-resistant (MDR) human non-small cell lung carcinoma (NSCLC) cells. All hybrid compounds with piperazine linker 16a–k were selective toward NSCLC cells and displayed IC50 values below 5 μM. Although they showed similar anticancer potency as artesunate, their selectivity against cancer cells was considerably improved. Importantly, 16h–k hybrid compounds were able to evade MDR phenotype, inhibit P-glycoprotein (P-gp) activity, and increase the sensitivity of MDR NSCLC cells to doxorubicin (DOX). The inhibition of P-gp activity induced by 16h–j was stronger than the one obtained with artesunate. Among these four hybrid compounds, 16k was the most potent anticancer agent with similar IC50 values of around 1.5 μM (for comparison – over 3.1 μM for artesunate) in sensitive and MDR NSCLC cells.",
publisher = "Royal Society of Chemistry",
journal = "New Journal of Chemistry",
title = "Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells",
volume = "47",
number = "14",
pages = "6844-6855",
doi = "10.1039/D3NJ00427A"
}

Koračak, L., Lupšić, E., Terzić-Jovanović, N., Jovanović, M., Novaković, M., Nedialkov, P., Trendafilova, A., Zlatović, M., Pešić, M.,& Opsenica, I.. (2023). Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells. in New Journal of Chemistry
Royal Society of Chemistry., 47(14), 6844-6855.
https://doi.org/10.1039/D3NJ00427A

Koračak L, Lupšić E, Terzić-Jovanović N, Jovanović M, Novaković M, Nedialkov P, Trendafilova A, Zlatović M, Pešić M, Opsenica I. Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells. in New Journal of Chemistry. 2023;47(14):6844-6855.
doi:10.1039/D3NJ00427A .

Koračak, Ljiljana, Lupšić, Ema, Terzić-Jovanović, Nataša, Jovanović, Mirna, Novaković, Miroslav, Nedialkov, Paraskev, Trendafilova, Antoaneta, Zlatović, Mario, Pešić, Milica, Opsenica, Igor, "Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells" in New Journal of Chemistry, 47, no. 14 (2023):6844-6855,
https://doi.org/10.1039/D3NJ00427A . .

TY  - JOUR
AU  - Stanković Jeremić, Jovana
AU  - Gođevac, Dejan
AU  - Ivanović, Stefan
AU  - Simić, Katarina
AU  - Trendafilova, Antoaneta
AU  - Aćimović, Milica
AU  - Milosavljević, Slobodan
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5166
AB  - The application of High Performance Thin Layer Chromatography
(HPTLC)-based non-targeted metabolomics as a holistic approach to compare
fingerprints of metabolite changes during Artemisia annua development is
described. Images of HPTLC chromatograms obtained after derivatization with
anisaldehyde-sulphuric acid reagent were used as a dataset for multivariate
analysis. Principal component analysis and Orthogonal Partial Least Squares
Discriminant Analysis confirmed the discrimination of samples belonging to
vegetative phase, flowering stage, and seed formation stage of the plant
development. The obtained results showed that the HPTLC-based metabolomics
approach can be a very reliable technique for the investigation of metabolic
changes during plant development, complementary to GC-MS and NMR-based
metabolomics.
AB  - Описана је холистичка примена нетаргетне метаболомике базиране на високоефикасној танкослојној хроматографији (HPTLC) која омогућава поређење метаболичких профила Artemisia annua и праћење њихових промена током развоја биљке. HPTLC хроматограми након развијања анизалдехидом и сумпорном киселином су коришћени за генерисање података за мултиваријантну анализу. Анализа главних компоненти (PCA) и ортогонална дискриминанта анализа најмањих квадрата (OPLS-DA) су потврдиле разлике између узорака који припадају различитим фенофазама - вегетативној фази, фази цветања и фази формирања семена. Добијени резултати указују да метаболомички приступ заснован на HPTLC методи која је комплементарна са GC-MS и NMR анализом, може бити веома поуздана техника за анализу промене током развоја биљке.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua
T1  - Mетаболомикa базиранa на HPTLC за испитивање метаболичких промена током развоја биљке:  Artemisia annua студија случаја
VL  - 87
IS  - 11
SP  - 1237
EP  - 1244
DO  - 10.2298/JSC210507007S
ER  - 

@article{
author = "Stanković Jeremić, Jovana and Gođevac, Dejan and Ivanović, Stefan and Simić, Katarina and Trendafilova, Antoaneta and Aćimović, Milica and Milosavljević, Slobodan",
year = "2022",
abstract = "The application of High Performance Thin Layer Chromatography
(HPTLC)-based non-targeted metabolomics as a holistic approach to compare
fingerprints of metabolite changes during Artemisia annua development is
described. Images of HPTLC chromatograms obtained after derivatization with
anisaldehyde-sulphuric acid reagent were used as a dataset for multivariate
analysis. Principal component analysis and Orthogonal Partial Least Squares
Discriminant Analysis confirmed the discrimination of samples belonging to
vegetative phase, flowering stage, and seed formation stage of the plant
development. The obtained results showed that the HPTLC-based metabolomics
approach can be a very reliable technique for the investigation of metabolic
changes during plant development, complementary to GC-MS and NMR-based
metabolomics., Описана је холистичка примена нетаргетне метаболомике базиране на високоефикасној танкослојној хроматографији (HPTLC) која омогућава поређење метаболичких профила Artemisia annua и праћење њихових промена током развоја биљке. HPTLC хроматограми након развијања анизалдехидом и сумпорном киселином су коришћени за генерисање података за мултиваријантну анализу. Анализа главних компоненти (PCA) и ортогонална дискриминанта анализа најмањих квадрата (OPLS-DA) су потврдиле разлике између узорака који припадају различитим фенофазама - вегетативној фази, фази цветања и фази формирања семена. Добијени резултати указују да метаболомички приступ заснован на HPTLC методи која је комплементарна са GC-MS и NMR анализом, може бити веома поуздана техника за анализу промене током развоја биљке.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua, Mетаболомикa базиранa на HPTLC за испитивање метаболичких промена током развоја биљке:  Artemisia annua студија случаја",
volume = "87",
number = "11",
pages = "1237-1244",
doi = "10.2298/JSC210507007S"
}

Stanković Jeremić, J., Gođevac, D., Ivanović, S., Simić, K., Trendafilova, A., Aćimović, M.,& Milosavljević, S.. (2022). HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 87(11), 1237-1244.
https://doi.org/10.2298/JSC210507007S

Stanković Jeremić J, Gođevac D, Ivanović S, Simić K, Trendafilova A, Aćimović M, Milosavljević S. HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua. in Journal of the Serbian Chemical Society. 2022;87(11):1237-1244.
doi:10.2298/JSC210507007S .

Stanković Jeremić, Jovana, Gođevac, Dejan, Ivanović, Stefan, Simić, Katarina, Trendafilova, Antoaneta, Aćimović, Milica, Milosavljević, Slobodan, "HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua" in Journal of the Serbian Chemical Society, 87, no. 11 (2022):1237-1244,
https://doi.org/10.2298/JSC210507007S . .

TY  - JOUR
AU  - Ozek, Gulmira
AU  - Yur, Suleyman
AU  - Goger, Fatih
AU  - Ozek, Temel
AU  - Anđelković, Boban D.
AU  - Gođevac, Dejan
AU  - Sofrenić, Ivana
AU  - Aneva, Ina
AU  - Todorova, Milka
AU  - Trendafilova, Antoaneta
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2869
AB  - Hexane extracts of Heracleum verticillatum, H. sibiricum, H. angustisectum, and H. ternatum were studied for their furanocoumarin content antioxidant potential and acetylcholinesterase and α-amylase inhibitory activities. Quantification of the furanocoumarins was performed by 1H-NMR. Pimpinellin was found to be the main component in the roots of all studied species. Bergapten and imperatorin were the major compounds in the fruits of H. sibiricum and H. verticillatum, respectively, while byakangelicol dominated in H. angustisectum and H. ternatum fruits. The leaf and fruit extracts of H. angustisectum demonstrated the highest DPPH radical scavenging activity and TEAC (IC50 0.58 mg/mL and 1.83 mM, respectively). The root extracts of H. verticillatum and H.angustisectum were found to be the most effective against acetylcholinesterase (IC50 0.30 and 0.34 mg/mL, respectively). The studied extracts were not active or demonstrated a weak inhibitory effect (%Inh. up to 29.7) towards α-amylase.
PB  - Willey
T2  - Chemistry and Biodiversity
T1  - Furanocoumarin Content, Antioxidant Activity, and Inhibitory Potential of Heracleum verticillatum, Heracleum sibiricum, Heracleum angustisectum, and Heracleum ternatum Extracts against Enzymes Involved in Alzheimer’s Disease and Type II Diabetes
VL  - 16
IS  - 4
SP  - e1800672
DO  - 10.1002/cbdv.201800672
ER  - 

@article{
author = "Ozek, Gulmira and Yur, Suleyman and Goger, Fatih and Ozek, Temel and Anđelković, Boban D. and Gođevac, Dejan and Sofrenić, Ivana and Aneva, Ina and Todorova, Milka and Trendafilova, Antoaneta",
year = "2019",
abstract = "Hexane extracts of Heracleum verticillatum, H. sibiricum, H. angustisectum, and H. ternatum were studied for their furanocoumarin content antioxidant potential and acetylcholinesterase and α-amylase inhibitory activities. Quantification of the furanocoumarins was performed by 1H-NMR. Pimpinellin was found to be the main component in the roots of all studied species. Bergapten and imperatorin were the major compounds in the fruits of H. sibiricum and H. verticillatum, respectively, while byakangelicol dominated in H. angustisectum and H. ternatum fruits. The leaf and fruit extracts of H. angustisectum demonstrated the highest DPPH radical scavenging activity and TEAC (IC50 0.58 mg/mL and 1.83 mM, respectively). The root extracts of H. verticillatum and H.angustisectum were found to be the most effective against acetylcholinesterase (IC50 0.30 and 0.34 mg/mL, respectively). The studied extracts were not active or demonstrated a weak inhibitory effect (%Inh. up to 29.7) towards α-amylase.",
publisher = "Willey",
journal = "Chemistry and Biodiversity",
title = "Furanocoumarin Content, Antioxidant Activity, and Inhibitory Potential of Heracleum verticillatum, Heracleum sibiricum, Heracleum angustisectum, and Heracleum ternatum Extracts against Enzymes Involved in Alzheimer’s Disease and Type II Diabetes",
volume = "16",
number = "4",
pages = "e1800672",
doi = "10.1002/cbdv.201800672"
}

Ozek, G., Yur, S., Goger, F., Ozek, T., Anđelković, B. D., Gođevac, D., Sofrenić, I., Aneva, I., Todorova, M.,& Trendafilova, A.. (2019). Furanocoumarin Content, Antioxidant Activity, and Inhibitory Potential of Heracleum verticillatum, Heracleum sibiricum, Heracleum angustisectum, and Heracleum ternatum Extracts against Enzymes Involved in Alzheimer’s Disease and Type II Diabetes. in Chemistry and Biodiversity
Willey., 16(4), e1800672.
https://doi.org/10.1002/cbdv.201800672

Ozek G, Yur S, Goger F, Ozek T, Anđelković BD, Gođevac D, Sofrenić I, Aneva I, Todorova M, Trendafilova A. Furanocoumarin Content, Antioxidant Activity, and Inhibitory Potential of Heracleum verticillatum, Heracleum sibiricum, Heracleum angustisectum, and Heracleum ternatum Extracts against Enzymes Involved in Alzheimer’s Disease and Type II Diabetes. in Chemistry and Biodiversity. 2019;16(4):e1800672.
doi:10.1002/cbdv.201800672 .

Ozek, Gulmira, Yur, Suleyman, Goger, Fatih, Ozek, Temel, Anđelković, Boban D., Gođevac, Dejan, Sofrenić, Ivana, Aneva, Ina, Todorova, Milka, Trendafilova, Antoaneta, "Furanocoumarin Content, Antioxidant Activity, and Inhibitory Potential of Heracleum verticillatum, Heracleum sibiricum, Heracleum angustisectum, and Heracleum ternatum Extracts against Enzymes Involved in Alzheimer’s Disease and Type II Diabetes" in Chemistry and Biodiversity, 16, no. 4 (2019):e1800672,
https://doi.org/10.1002/cbdv.201800672 . .

TY  - JOUR
AU  - Ozek, Gulmira
AU  - Yur, Suleyman
AU  - Goger, Fatih
AU  - Ozek, Temel
AU  - Anđelković, Boban D.
AU  - Gođevac, Dejan
AU  - Sofrenić, Ivana
AU  - Aneva, Ina
AU  - Todorova, Milka
AU  - Trendafilova, Antoaneta
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2869
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2899
AB  - Hexane extracts of Heracleum verticillatum, H. sibiricum, H. angustisectum, and H. ternatum were studied fortheir furanocoumarin content antioxidant potential and acetylcholinesterase and α-amylase inhibitory activities.Quantification of the furanocoumarins was performed by 1H-NMR. Pimpinellin was found to be the maincomponent in the roots of all studied species. Bergapten and imperatorin were the major compounds in thefruits of H. sibiricum and H. verticillatum, respectively, while byakangelicol dominated in H. angustisectum and H.ternatum fruits. The leaf and fruit extracts of H. angustisectum demonstrated the highest DPPH radical scavengingactivity and TEAC (IC50 0.58 mg/mL and 1.83 mM, respectively). The root extracts of H. verticillatum and H.angustisectum were found to be the most effective against acetylcholinesterase (IC50 0.30 and 0.34 mg/mL,respectively). The studied extracts were not active or demonstrated a weak inhibitory effect (%Inh. up to 29.7)towards α-amylase.
PB  - Willey
T2  - Chemistry and Biodiversity
T1  - Furanocoumarin Content, Antioxidant Activity, and Inhibitory Potential of Heracleum verticillatum, Heracleum sibiricum, Heracleum angustisectum, and Heracleum ternatum Extracts against Enzymes Involved in Alzheimer’s Disease and Type II Diabetes
VL  - 16
IS  - 4
SP  - e1800672
DO  - 10.1002/cbdv.201800672
ER  - 

@article{
author = "Ozek, Gulmira and Yur, Suleyman and Goger, Fatih and Ozek, Temel and Anđelković, Boban D. and Gođevac, Dejan and Sofrenić, Ivana and Aneva, Ina and Todorova, Milka and Trendafilova, Antoaneta",
year = "2019",
abstract = "Hexane extracts of Heracleum verticillatum, H. sibiricum, H. angustisectum, and H. ternatum were studied fortheir furanocoumarin content antioxidant potential and acetylcholinesterase and α-amylase inhibitory activities.Quantification of the furanocoumarins was performed by 1H-NMR. Pimpinellin was found to be the maincomponent in the roots of all studied species. Bergapten and imperatorin were the major compounds in thefruits of H. sibiricum and H. verticillatum, respectively, while byakangelicol dominated in H. angustisectum and H.ternatum fruits. The leaf and fruit extracts of H. angustisectum demonstrated the highest DPPH radical scavengingactivity and TEAC (IC50 0.58 mg/mL and 1.83 mM, respectively). The root extracts of H. verticillatum and H.angustisectum were found to be the most effective against acetylcholinesterase (IC50 0.30 and 0.34 mg/mL,respectively). The studied extracts were not active or demonstrated a weak inhibitory effect (%Inh. up to 29.7)towards α-amylase.",
publisher = "Willey",
journal = "Chemistry and Biodiversity",
title = "Furanocoumarin Content, Antioxidant Activity, and Inhibitory Potential of Heracleum verticillatum, Heracleum sibiricum, Heracleum angustisectum, and Heracleum ternatum Extracts against Enzymes Involved in Alzheimer’s Disease and Type II Diabetes",
volume = "16",
number = "4",
pages = "e1800672",
doi = "10.1002/cbdv.201800672"
}

Ozek, G., Yur, S., Goger, F., Ozek, T., Anđelković, B. D., Gođevac, D., Sofrenić, I., Aneva, I., Todorova, M.,& Trendafilova, A.. (2019). Furanocoumarin Content, Antioxidant Activity, and Inhibitory Potential of Heracleum verticillatum, Heracleum sibiricum, Heracleum angustisectum, and Heracleum ternatum Extracts against Enzymes Involved in Alzheimer’s Disease and Type II Diabetes. in Chemistry and Biodiversity
Willey., 16(4), e1800672.
https://doi.org/10.1002/cbdv.201800672

Ozek G, Yur S, Goger F, Ozek T, Anđelković BD, Gođevac D, Sofrenić I, Aneva I, Todorova M, Trendafilova A. Furanocoumarin Content, Antioxidant Activity, and Inhibitory Potential of Heracleum verticillatum, Heracleum sibiricum, Heracleum angustisectum, and Heracleum ternatum Extracts against Enzymes Involved in Alzheimer’s Disease and Type II Diabetes. in Chemistry and Biodiversity. 2019;16(4):e1800672.
doi:10.1002/cbdv.201800672 .

Ozek, Gulmira, Yur, Suleyman, Goger, Fatih, Ozek, Temel, Anđelković, Boban D., Gođevac, Dejan, Sofrenić, Ivana, Aneva, Ina, Todorova, Milka, Trendafilova, Antoaneta, "Furanocoumarin Content, Antioxidant Activity, and Inhibitory Potential of Heracleum verticillatum, Heracleum sibiricum, Heracleum angustisectum, and Heracleum ternatum Extracts against Enzymes Involved in Alzheimer’s Disease and Type II Diabetes" in Chemistry and Biodiversity, 16, no. 4 (2019):e1800672,
https://doi.org/10.1002/cbdv.201800672 . .

TY  - JOUR
AU  - Trendafilova, Antoaneta
AU  - Jadranin, Milka
AU  - Gorgorov, Rossen
AU  - Stanilova, Marina
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1653
AB  - In vitro cultures were initiated from a single seed of Centaurea davidovii. Whole plantlets were regenerated and cultivated for several months on agar-solidified nutrient media differing by their composition: basal MS medium, MS medium supplemented with plant growth regulators, and liquid MS medium. Plantlets were ex vitro adapted and successfully acclimated to open-air conditions; flowering was observed in some individuals in the first summer, and mass flowering during the second summer. The contents of the total flavonoids and the total phenolic compounds were determined spectrophotometrically in the leaves of the in vitro plantlets cultured on different media, and then compared with those in the leaves of the wild plants and in the leaves of the acclimated plants of the field plot. The sesquiterpene lactone 8 alpha-(5'-hydroxyangeloyl)-salonitenolide was determined by HPLC in leaf samples of C. davidovii wild plants, in vitro obtained plantlets and ex vitro acclimated plants in the greenhouse and on the experimental field plot. The composition of the nutrient medium influenced the contents of all studied bioactive substances. The highest concentrations of all tested secondary metabolites were detected in the leaves of the acclimated plants during mass flowering, the content of the lactone reaching 56.2 mg/g DW, which was several times more than in the other leaf samples. The obtained results revealed both the effectiveness of biotechnological methods for propagation and conservation of rare and endangered plant species, and the possibility to use C. davidovii plants ex vitro acclimated to field conditions as a source of secondary metabolites with potential biological activity.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - Bioactive Compounds in Wild, In vitro Obtained, Ex vitro Adapted, and Acclimated Plants of Centaurea davidovii (Asteraceae)
VL  - 10
IS  - 6
SP  - 839
EP  - 841
UR  - https://hdl.handle.net/21.15107/rcub_cer_1653
ER  - 

@article{
author = "Trendafilova, Antoaneta and Jadranin, Milka and Gorgorov, Rossen and Stanilova, Marina",
year = "2015",
abstract = "In vitro cultures were initiated from a single seed of Centaurea davidovii. Whole plantlets were regenerated and cultivated for several months on agar-solidified nutrient media differing by their composition: basal MS medium, MS medium supplemented with plant growth regulators, and liquid MS medium. Plantlets were ex vitro adapted and successfully acclimated to open-air conditions; flowering was observed in some individuals in the first summer, and mass flowering during the second summer. The contents of the total flavonoids and the total phenolic compounds were determined spectrophotometrically in the leaves of the in vitro plantlets cultured on different media, and then compared with those in the leaves of the wild plants and in the leaves of the acclimated plants of the field plot. The sesquiterpene lactone 8 alpha-(5'-hydroxyangeloyl)-salonitenolide was determined by HPLC in leaf samples of C. davidovii wild plants, in vitro obtained plantlets and ex vitro acclimated plants in the greenhouse and on the experimental field plot. The composition of the nutrient medium influenced the contents of all studied bioactive substances. The highest concentrations of all tested secondary metabolites were detected in the leaves of the acclimated plants during mass flowering, the content of the lactone reaching 56.2 mg/g DW, which was several times more than in the other leaf samples. The obtained results revealed both the effectiveness of biotechnological methods for propagation and conservation of rare and endangered plant species, and the possibility to use C. davidovii plants ex vitro acclimated to field conditions as a source of secondary metabolites with potential biological activity.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Bioactive Compounds in Wild, In vitro Obtained, Ex vitro Adapted, and Acclimated Plants of Centaurea davidovii (Asteraceae)",
volume = "10",
number = "6",
pages = "839-841",
url = "https://hdl.handle.net/21.15107/rcub_cer_1653"
}

Trendafilova, A., Jadranin, M., Gorgorov, R.,& Stanilova, M.. (2015). Bioactive Compounds in Wild, In vitro Obtained, Ex vitro Adapted, and Acclimated Plants of Centaurea davidovii (Asteraceae). in Natural Product Communications
Natural Products Inc, Westerville., 10(6), 839-841.
https://hdl.handle.net/21.15107/rcub_cer_1653

Trendafilova A, Jadranin M, Gorgorov R, Stanilova M. Bioactive Compounds in Wild, In vitro Obtained, Ex vitro Adapted, and Acclimated Plants of Centaurea davidovii (Asteraceae). in Natural Product Communications. 2015;10(6):839-841.
https://hdl.handle.net/21.15107/rcub_cer_1653 .

Trendafilova, Antoaneta, Jadranin, Milka, Gorgorov, Rossen, Stanilova, Marina, "Bioactive Compounds in Wild, In vitro Obtained, Ex vitro Adapted, and Acclimated Plants of Centaurea davidovii (Asteraceae)" in Natural Product Communications, 10, no. 6 (2015):839-841,
https://hdl.handle.net/21.15107/rcub_cer_1653 .

TY  - JOUR
AU  - Trendafilova, Antoaneta
AU  - Todorova, Milka
AU  - Genova, Viktoriya
AU  - Shestakova, Pavletta
AU  - Dimitrov, Dimitar
AU  - Jadranin, Milka
AU  - Milosavljević, Slobodan
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1562
AB  - The aerial parts of Inula aschersoniana Janka var. aschersoniana afforded parthenolide, diepoxycostunolide, inusoniolide, chrysosplenol C and four new pseudoguaiane-type sesquiterpenoids. Their structures were determined using spectral methods and relative stereochemistry by NOESY correlations.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - New Pseudoguaiane Derivatives from Inula aschersoniana Janka var. aschersoniana
VL  - 9
IS  - 8
SP  - 1123
EP  - 1124
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1829
ER  - 

@article{
author = "Trendafilova, Antoaneta and Todorova, Milka and Genova, Viktoriya and Shestakova, Pavletta and Dimitrov, Dimitar and Jadranin, Milka and Milosavljević, Slobodan",
year = "2014",
abstract = "The aerial parts of Inula aschersoniana Janka var. aschersoniana afforded parthenolide, diepoxycostunolide, inusoniolide, chrysosplenol C and four new pseudoguaiane-type sesquiterpenoids. Their structures were determined using spectral methods and relative stereochemistry by NOESY correlations.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "New Pseudoguaiane Derivatives from Inula aschersoniana Janka var. aschersoniana",
volume = "9",
number = "8",
pages = "1123-1124",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1829"
}

Trendafilova, A., Todorova, M., Genova, V., Shestakova, P., Dimitrov, D., Jadranin, M.,& Milosavljević, S.. (2014). New Pseudoguaiane Derivatives from Inula aschersoniana Janka var. aschersoniana. in Natural Product Communications
Natural Products Inc, Westerville., 9(8), 1123-1124.
https://hdl.handle.net/21.15107/rcub_cherry_1829

Trendafilova A, Todorova M, Genova V, Shestakova P, Dimitrov D, Jadranin M, Milosavljević S. New Pseudoguaiane Derivatives from Inula aschersoniana Janka var. aschersoniana. in Natural Product Communications. 2014;9(8):1123-1124.
https://hdl.handle.net/21.15107/rcub_cherry_1829 .

Trendafilova, Antoaneta, Todorova, Milka, Genova, Viktoriya, Shestakova, Pavletta, Dimitrov, Dimitar, Jadranin, Milka, Milosavljević, Slobodan, "New Pseudoguaiane Derivatives from Inula aschersoniana Janka var. aschersoniana" in Natural Product Communications, 9, no. 8 (2014):1123-1124,
https://hdl.handle.net/21.15107/rcub_cherry_1829 .