@article{
author = "Prlainović, Nevena Ž. and Bezbradica, Dejan I. and Knežević-Jugović, Zorica and Stevanović, Sanja and Avramov Ivić, Milka and Uskoković, Petar S. and Mijin, Dušan",
year = "2013",
abstract = "In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCIsIT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.",
publisher = "Korean Society of Industrial Engineering Chemistry",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes",
volume = "19",
number = "1",
pages = "279-285",
doi = "10.1016/j.jiec.2012.08.012"
}