TY  - JOUR
AU  - Janković, Smiljana
AU  - Alimpić Aradski,  Ana
AU  - Dodoš, Tanja
AU  - Novaković, Jelica
AU  - Ivanović, Stefan
AU  - Vujisić, Ljubodrag
AU  - Marin, Petar
AU  - Rajčević, Nemanja
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7417
AB  - The concept of the genus Clinopodium L. has changed considerably since its first description. Most of the currently accepted species of the genus have traditionally been treated as separate genera in the group Satureja sensu lato: Clinopodium L., Calamintha sensu Miller or Moench, and Acinos sensu Miller or Moench. This study aimed to gain a better insight into the species diversity of Clinopodium L. from the Balkans by analyzing the taxa that have traditionally been placed in separate genera. The alkane profile and the micromorphological characteristics of the leaves are analyzed. The leaves are visualized using scanning electron microscopy, and alkanes are isolated using n-hexane as a solvent and analyzed using gas chromatography/mass spectrometry. The alkane profile showed the differentiation of the Acinos-group from the other taxa based on the dominant n-C31, while most of the other taxa contained n-C33 as the dominant alkane. The micromorphological features also showed clear differences between the previously recognized genera, especially in the capitate trichomes. The results showed that micromorphological patterns are highly variable in certain groups of the genus Clinopodium.
PB  - MDP
T2  - Plants
T1  - Clinopodium L. Taxa from the Balkans - Are There Unique Leaf Micromorphological and Phytochemical Patterns?
VL  - 13
IS  - 2
SP  - 251
DO  - 10.3390/plants13020251
ER  - 

@article{
author = "Janković, Smiljana and Alimpić Aradski,  Ana and Dodoš, Tanja and Novaković, Jelica and Ivanović, Stefan and Vujisić, Ljubodrag and Marin, Petar and Rajčević, Nemanja",
year = "2024",
abstract = "The concept of the genus Clinopodium L. has changed considerably since its first description. Most of the currently accepted species of the genus have traditionally been treated as separate genera in the group Satureja sensu lato: Clinopodium L., Calamintha sensu Miller or Moench, and Acinos sensu Miller or Moench. This study aimed to gain a better insight into the species diversity of Clinopodium L. from the Balkans by analyzing the taxa that have traditionally been placed in separate genera. The alkane profile and the micromorphological characteristics of the leaves are analyzed. The leaves are visualized using scanning electron microscopy, and alkanes are isolated using n-hexane as a solvent and analyzed using gas chromatography/mass spectrometry. The alkane profile showed the differentiation of the Acinos-group from the other taxa based on the dominant n-C31, while most of the other taxa contained n-C33 as the dominant alkane. The micromorphological features also showed clear differences between the previously recognized genera, especially in the capitate trichomes. The results showed that micromorphological patterns are highly variable in certain groups of the genus Clinopodium.",
publisher = "MDP",
journal = "Plants",
title = "Clinopodium L. Taxa from the Balkans - Are There Unique Leaf Micromorphological and Phytochemical Patterns?",
volume = "13",
number = "2",
pages = "251",
doi = "10.3390/plants13020251"
}

Janković, S., Alimpić Aradski, A., Dodoš, T., Novaković, J., Ivanović, S., Vujisić, L., Marin, P.,& Rajčević, N.. (2024). Clinopodium L. Taxa from the Balkans - Are There Unique Leaf Micromorphological and Phytochemical Patterns?. in Plants
MDP., 13(2), 251.
https://doi.org/10.3390/plants13020251

Janković S, Alimpić Aradski A, Dodoš T, Novaković J, Ivanović S, Vujisić L, Marin P, Rajčević N. Clinopodium L. Taxa from the Balkans - Are There Unique Leaf Micromorphological and Phytochemical Patterns?. in Plants. 2024;13(2):251.
doi:10.3390/plants13020251 .

Janković, Smiljana, Alimpić Aradski,  Ana, Dodoš, Tanja, Novaković, Jelica, Ivanović, Stefan, Vujisić, Ljubodrag, Marin, Petar, Rajčević, Nemanja, "Clinopodium L. Taxa from the Balkans - Are There Unique Leaf Micromorphological and Phytochemical Patterns?" in Plants, 13, no. 2 (2024):251,
https://doi.org/10.3390/plants13020251 . .

TY  - JOUR
AU  - Zdunić, Gordana
AU  - Aradski, Ana Alimpić
AU  - Gođevac, Dejan
AU  - Živković, Jelena Č.
AU  - Laušević, Sonja Duletić
AU  - Milošević, Dijana Krstić
AU  - Šavikin, Katarina
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3446
AB  - Chokeberries (Aronia melanocarpa (Michx.) Elliot have recently become one of the most popular and widely used berry fruits, and there are numerous studies dealing with the investigation of their chemical composition and biological activities. Unlike berries, chokeberry leaves were far less investigated. Therefore, the aim of this study was to conduct a bioactivity-based analysis of chokeberry leaves collected after berries were harvested. Chemical analysis of hydroethanolic chokeberry extract and its ethyl acetate, n-butanol, and water fractions was performed. Antioxidant, hypoglycemic, and antineurodegenerative activities of the obtained samples were assessed by in vitro tests. The highest total phenolics content was found in n-butanol fraction (221.5 mg gallic acid equivalents/g dw). Fourteen compounds (six phenolic acids and eight flavonoids) were identified in the samples. Caffeoylquinic acid derivatives and quercetin glycosides were dominant phenolic compounds. The highest content of phenolic acids, as well as flavonoids, was recorded in n-butanol fraction (80.75 mg/g dw and 47.79 mg/g dw, respectively). This fraction was the most active in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. Also, the n-butanol fraction was the most effective inhibitor of acetylcholinesterase (AChE) and tyrosinase (TYR) enzymes. All samples showed a better inhibitory effect on α-glucosidase enzyme with inhibition concentrations IC50 = 4.21 - 9.22 μg/mL, than α-amylase enzymes (IC50 = 14.36 - 88.61 μg/mL). Pearson’s correlation between the content of individual phenolics and the results of biological tests was performed. Thus, hyperoside and isoquercitrin showed a significant correlation with DPPH and ABTS tests, caffeoylquinic acids with FRAP test, quercetin 3-O-vicianoside with β-carotene assay, hyperoside and isoquercitrin with α-amylase inhibition activity, and isorhamnetin 3-O-rutinoside with α-glucosidase inhibition assay. Considering the obtained results, chokeberry leaves represent a valuable natural source of phenolic compounds with promising potential for the development of new products with beneficial effects on human health.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves
VL  - 148
SP  - 112328
DO  - 10.1016/j.indcrop.2020.112328
ER  - 

@article{
author = "Zdunić, Gordana and Aradski, Ana Alimpić and Gođevac, Dejan and Živković, Jelena Č. and Laušević, Sonja Duletić and Milošević, Dijana Krstić and Šavikin, Katarina",
year = "2020",
abstract = "Chokeberries (Aronia melanocarpa (Michx.) Elliot have recently become one of the most popular and widely used berry fruits, and there are numerous studies dealing with the investigation of their chemical composition and biological activities. Unlike berries, chokeberry leaves were far less investigated. Therefore, the aim of this study was to conduct a bioactivity-based analysis of chokeberry leaves collected after berries were harvested. Chemical analysis of hydroethanolic chokeberry extract and its ethyl acetate, n-butanol, and water fractions was performed. Antioxidant, hypoglycemic, and antineurodegenerative activities of the obtained samples were assessed by in vitro tests. The highest total phenolics content was found in n-butanol fraction (221.5 mg gallic acid equivalents/g dw). Fourteen compounds (six phenolic acids and eight flavonoids) were identified in the samples. Caffeoylquinic acid derivatives and quercetin glycosides were dominant phenolic compounds. The highest content of phenolic acids, as well as flavonoids, was recorded in n-butanol fraction (80.75 mg/g dw and 47.79 mg/g dw, respectively). This fraction was the most active in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. Also, the n-butanol fraction was the most effective inhibitor of acetylcholinesterase (AChE) and tyrosinase (TYR) enzymes. All samples showed a better inhibitory effect on α-glucosidase enzyme with inhibition concentrations IC50 = 4.21 - 9.22 μg/mL, than α-amylase enzymes (IC50 = 14.36 - 88.61 μg/mL). Pearson’s correlation between the content of individual phenolics and the results of biological tests was performed. Thus, hyperoside and isoquercitrin showed a significant correlation with DPPH and ABTS tests, caffeoylquinic acids with FRAP test, quercetin 3-O-vicianoside with β-carotene assay, hyperoside and isoquercitrin with α-amylase inhibition activity, and isorhamnetin 3-O-rutinoside with α-glucosidase inhibition assay. Considering the obtained results, chokeberry leaves represent a valuable natural source of phenolic compounds with promising potential for the development of new products with beneficial effects on human health.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves",
volume = "148",
pages = "112328",
doi = "10.1016/j.indcrop.2020.112328"
}

Zdunić, G., Aradski, A. A., Gođevac, D., Živković, J. Č., Laušević, S. D., Milošević, D. K.,& Šavikin, K.. (2020). In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves. in Industrial Crops and Products
Elsevier., 148, 112328.
https://doi.org/10.1016/j.indcrop.2020.112328

Zdunić G, Aradski AA, Gođevac D, Živković JČ, Laušević SD, Milošević DK, Šavikin K. In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves. in Industrial Crops and Products. 2020;148:112328.
doi:10.1016/j.indcrop.2020.112328 .

Zdunić, Gordana, Aradski, Ana Alimpić, Gođevac, Dejan, Živković, Jelena Č., Laušević, Sonja Duletić, Milošević, Dijana Krstić, Šavikin, Katarina, "In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves" in Industrial Crops and Products, 148 (2020):112328,
https://doi.org/10.1016/j.indcrop.2020.112328 . .