TY  - JOUR
AU  - Đorđević, J.
AU  - Kolarević, S.
AU  - Jovanović, J.
AU  - Kostić-Vuković, J.
AU  - Novaković, Irena
AU  - Jeremić, M.
AU  - Sladić, Dušan
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2415
AB  - Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents.
PB  - Taylor and Francis Ltd
T2  - Drug and Chemical Toxicology
T1  - Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models
SP  - 522
EP  - 530
DO  - 10.1080/01480545.2018.1514043
ER  - 

@article{
author = "Đorđević, J. and Kolarević, S. and Jovanović, J. and Kostić-Vuković, J. and Novaković, Irena and Jeremić, M. and Sladić, Dušan and Vuković-Gačić, Branka",
year = "2018",
abstract = "Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents.",
publisher = "Taylor and Francis Ltd",
journal = "Drug and Chemical Toxicology",
title = "Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models",
pages = "522-530",
doi = "10.1080/01480545.2018.1514043"
}

Đorđević, J., Kolarević, S., Jovanović, J., Kostić-Vuković, J., Novaković, I., Jeremić, M., Sladić, D.,& Vuković-Gačić, B.. (2018). Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology
Taylor and Francis Ltd., 522-530.
https://doi.org/10.1080/01480545.2018.1514043

Đorđević J, Kolarević S, Jovanović J, Kostić-Vuković J, Novaković I, Jeremić M, Sladić D, Vuković-Gačić B. Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology. 2018;:522-530.
doi:10.1080/01480545.2018.1514043 .

Đorđević, J., Kolarević, S., Jovanović, J., Kostić-Vuković, J., Novaković, Irena, Jeremić, M., Sladić, Dušan, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of tert-butylquinone and its derivatives in prokaryotic and eukaryotic test models" in Drug and Chemical Toxicology (2018):522-530,
https://doi.org/10.1080/01480545.2018.1514043 . .

TY  - JOUR
AU  - Kolarević, Stoimir M.
AU  - Milovanović, Dragana
AU  - Kračun-Kolarević, Margareta
AU  - Kostić, Jovana
AU  - Sunjog, K.
AU  - Martinović, Rajko
AU  - Đorđević, Jelena
AU  - Novaković, Irena
AU  - Sladić, Dušan
AU  - Vuković-Gačić, Branka
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2232
AB  - In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3′-methoxyavarone, 4′-(methylamino)avarone and 3′-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3′-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3′-methoxyavarone and 3′-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.
PB  - Taylor and Francis Ltd
T2  - Drug and Chemical Toxicology
T1  - Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models
SP  - 1
EP  - 10
DO  - 10.1080/01480545.2017.1413108
ER  - 

@article{
author = "Kolarević, Stoimir M. and Milovanović, Dragana and Kračun-Kolarević, Margareta and Kostić, Jovana and Sunjog, K. and Martinović, Rajko and Đorđević, Jelena and Novaković, Irena and Sladić, Dušan and Vuković-Gačić, Branka",
year = "2017",
abstract = "In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3′-methoxyavarone, 4′-(methylamino)avarone and 3′-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3′-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3′-methoxyavarone and 3′-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.",
publisher = "Taylor and Francis Ltd",
journal = "Drug and Chemical Toxicology",
title = "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models",
pages = "1-10",
doi = "10.1080/01480545.2017.1413108"
}

Kolarević, S. M., Milovanović, D., Kračun-Kolarević, M., Kostić, J., Sunjog, K., Martinović, R., Đorđević, J., Novaković, I., Sladić, D.,& Vuković-Gačić, B.. (2017). Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology
Taylor and Francis Ltd., 1-10.
https://doi.org/10.1080/01480545.2017.1413108

Kolarević SM, Milovanović D, Kračun-Kolarević M, Kostić J, Sunjog K, Martinović R, Đorđević J, Novaković I, Sladić D, Vuković-Gačić B. Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology. 2017;:1-10.
doi:10.1080/01480545.2017.1413108 .

Kolarević, Stoimir M., Milovanović, Dragana, Kračun-Kolarević, Margareta, Kostić, Jovana, Sunjog, K., Martinović, Rajko, Đorđević, Jelena, Novaković, Irena, Sladić, Dušan, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models" in Drug and Chemical Toxicology (2017):1-10,
https://doi.org/10.1080/01480545.2017.1413108 . .

TY  - JOUR
AU  - Marin, Marija
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Kolarević, Stoimir M.
AU  - Vuković-Gačić, Branka
PY  - 2015
UR  - http://www.jbb.uni-plovdiv.bg/
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3970
AB  - The genus Micromeria Benth. (Lamiaceae, Nepetoideae) includes about 130 species, often aromatc. The essential oil and extracts of some Micromeria species have significant antioxidant, antibacterial and antifungal activities. Micromeria thymifolia is endemic species of the Balkan peninsula. It has been traditionally used in the Mediterranean area as condiment and medicinal plant. The aim of this study was to investigate antimicrobial properties of essential oil of wild Micromeria thymifolia against four Gram negative bacteria (Escherichia coli SY252, Pseudomonas aeruginosa ATCC27853, Salmonella enterica ATCC13076 and human patogen Burkholderia cepacia ATCC25416), four Gram positive bacteria (Enterococcus fecalis ATCC29212, Staphylococcus aureus ATCC25923, Bacillus subtilis ATCC6633, Listeria innocua ATCC33090) and two fungi strains (Candida albicans ATCC10231 and Saccharomyces cerevisiae ATCC9763). The MICs of M. thymifolia essential oil against tested bacteria and fungi was assessed using microtitre plate-based antimicrobial assay. MHB was used as growth media for bacteria, with exception of L.innocua when BHI was used, YPD was used for fungi. The results of our investigation showed that the essential oil of wild-growing M. thymifolia possess significant antimicrobial activity against all tested strains except the P. aeruginosa.
PB  - Bulgaria : Plovdiv University Press
T2  - Journal of BioScience and Biotechnology
T1  - Antimicrobial activity of the essential oil of wild-growing Micromeria thymifolia (Scop.) Fritsch
VL  - 4
IS  - 1
SP  - 29
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_cer_3970
ER  - 

@article{
author = "Marin, Marija and Novaković, Miroslav and Tešević, Vele and Kolarević, Stoimir M. and Vuković-Gačić, Branka",
year = "2015",
abstract = "The genus Micromeria Benth. (Lamiaceae, Nepetoideae) includes about 130 species, often aromatc. The essential oil and extracts of some Micromeria species have significant antioxidant, antibacterial and antifungal activities. Micromeria thymifolia is endemic species of the Balkan peninsula. It has been traditionally used in the Mediterranean area as condiment and medicinal plant. The aim of this study was to investigate antimicrobial properties of essential oil of wild Micromeria thymifolia against four Gram negative bacteria (Escherichia coli SY252, Pseudomonas aeruginosa ATCC27853, Salmonella enterica ATCC13076 and human patogen Burkholderia cepacia ATCC25416), four Gram positive bacteria (Enterococcus fecalis ATCC29212, Staphylococcus aureus ATCC25923, Bacillus subtilis ATCC6633, Listeria innocua ATCC33090) and two fungi strains (Candida albicans ATCC10231 and Saccharomyces cerevisiae ATCC9763). The MICs of M. thymifolia essential oil against tested bacteria and fungi was assessed using microtitre plate-based antimicrobial assay. MHB was used as growth media for bacteria, with exception of L.innocua when BHI was used, YPD was used for fungi. The results of our investigation showed that the essential oil of wild-growing M. thymifolia possess significant antimicrobial activity against all tested strains except the P. aeruginosa.",
publisher = "Bulgaria : Plovdiv University Press",
journal = "Journal of BioScience and Biotechnology",
title = "Antimicrobial activity of the essential oil of wild-growing Micromeria thymifolia (Scop.) Fritsch",
volume = "4",
number = "1",
pages = "29-31",
url = "https://hdl.handle.net/21.15107/rcub_cer_3970"
}

Marin, M., Novaković, M., Tešević, V., Kolarević, S. M.,& Vuković-Gačić, B.. (2015). Antimicrobial activity of the essential oil of wild-growing Micromeria thymifolia (Scop.) Fritsch. in Journal of BioScience and Biotechnology
Bulgaria : Plovdiv University Press., 4(1), 29-31.
https://hdl.handle.net/21.15107/rcub_cer_3970

Marin M, Novaković M, Tešević V, Kolarević SM, Vuković-Gačić B. Antimicrobial activity of the essential oil of wild-growing Micromeria thymifolia (Scop.) Fritsch. in Journal of BioScience and Biotechnology. 2015;4(1):29-31.
https://hdl.handle.net/21.15107/rcub_cer_3970 .

Marin, Marija, Novaković, Miroslav, Tešević, Vele, Kolarević, Stoimir M., Vuković-Gačić, Branka, "Antimicrobial activity of the essential oil of wild-growing Micromeria thymifolia (Scop.) Fritsch" in Journal of BioScience and Biotechnology, 4, no. 1 (2015):29-31,
https://hdl.handle.net/21.15107/rcub_cer_3970 .

TY  - JOUR
AU  - Marin, Marija
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Vučković, Ivan
AU  - Milojevic, Natasa
AU  - Vukovic-Gacic, Branka
AU  - Marin, Petar D.
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/965
AB  - The composition of the essential oil of Satureja montana was examined by gas chromatographymass spectrometry and 1H nuclear magnetic resonance spectroscopy. Forty-three compounds were identified, representing approximately 99% of the oil. Major components were ?-terpinene (8.7%), p-cymene (16.9%) and carvacrol (44.5%). The oil was tested for its antioxidant activity by using the 1,1-diphenyl-2-picryl hydrazyl assay and showed dose-dependent free-radical-scavenging activity with an EC50 value of 4.21 mg/ml. The antimicrobial effect of the essential oil was tested against six strains of Gram-negative bacteria, seven strains of Gram-positive bacteria and two fungi. The human pathogens Burkholderia cepacia ATCC 25416 (Gram negative) and Listeria innocua (Gram positive) were used in the investigation of S. montana for the first time. The minimum inhibitory concentration of the essential oil ranged from 0.625 to 5.0 mu l/well for Gram-negative bacteria, and from 0.312 to 1.125 mu l/well for Gram-positive bacteria. For the two fungi tested, Candida and Saccharomyces, the value was 0.234 mu l/tube. The essential oil tested showed significant activity against fungi and Gram-positive bacteria, especially Bacillus subtilis, Staphylococcus epidermidis and Listeria innocua, and among Gram-negative bacteria extreme sensitivity was detected in Escherichia coli SY252, SY252 lpcA and ATTCC 25922 strains. Copyright
PB  - Wiley-Blackwell, Malden
T2  - Flavour and Fragrance Journal
T1  - Antioxidative, antibacterial and antifungal activity of the essential oil of wild-growing Satureja montana L. from Dalmatia, Croatia
VL  - 27
IS  - 3
SP  - 216
EP  - 223
DO  - 10.1002/ffj.3082
ER  - 

@article{
author = "Marin, Marija and Novaković, Miroslav and Tešević, Vele and Vučković, Ivan and Milojevic, Natasa and Vukovic-Gacic, Branka and Marin, Petar D.",
year = "2012",
abstract = "The composition of the essential oil of Satureja montana was examined by gas chromatographymass spectrometry and 1H nuclear magnetic resonance spectroscopy. Forty-three compounds were identified, representing approximately 99% of the oil. Major components were ?-terpinene (8.7%), p-cymene (16.9%) and carvacrol (44.5%). The oil was tested for its antioxidant activity by using the 1,1-diphenyl-2-picryl hydrazyl assay and showed dose-dependent free-radical-scavenging activity with an EC50 value of 4.21 mg/ml. The antimicrobial effect of the essential oil was tested against six strains of Gram-negative bacteria, seven strains of Gram-positive bacteria and two fungi. The human pathogens Burkholderia cepacia ATCC 25416 (Gram negative) and Listeria innocua (Gram positive) were used in the investigation of S. montana for the first time. The minimum inhibitory concentration of the essential oil ranged from 0.625 to 5.0 mu l/well for Gram-negative bacteria, and from 0.312 to 1.125 mu l/well for Gram-positive bacteria. For the two fungi tested, Candida and Saccharomyces, the value was 0.234 mu l/tube. The essential oil tested showed significant activity against fungi and Gram-positive bacteria, especially Bacillus subtilis, Staphylococcus epidermidis and Listeria innocua, and among Gram-negative bacteria extreme sensitivity was detected in Escherichia coli SY252, SY252 lpcA and ATTCC 25922 strains. Copyright",
publisher = "Wiley-Blackwell, Malden",
journal = "Flavour and Fragrance Journal",
title = "Antioxidative, antibacterial and antifungal activity of the essential oil of wild-growing Satureja montana L. from Dalmatia, Croatia",
volume = "27",
number = "3",
pages = "216-223",
doi = "10.1002/ffj.3082"
}

Marin, M., Novaković, M., Tešević, V., Vučković, I., Milojevic, N., Vukovic-Gacic, B.,& Marin, P. D.. (2012). Antioxidative, antibacterial and antifungal activity of the essential oil of wild-growing Satureja montana L. from Dalmatia, Croatia. in Flavour and Fragrance Journal
Wiley-Blackwell, Malden., 27(3), 216-223.
https://doi.org/10.1002/ffj.3082

Marin M, Novaković M, Tešević V, Vučković I, Milojevic N, Vukovic-Gacic B, Marin PD. Antioxidative, antibacterial and antifungal activity of the essential oil of wild-growing Satureja montana L. from Dalmatia, Croatia. in Flavour and Fragrance Journal. 2012;27(3):216-223.
doi:10.1002/ffj.3082 .

Marin, Marija, Novaković, Miroslav, Tešević, Vele, Vučković, Ivan, Milojevic, Natasa, Vukovic-Gacic, Branka, Marin, Petar D., "Antioxidative, antibacterial and antifungal activity of the essential oil of wild-growing Satureja montana L. from Dalmatia, Croatia" in Flavour and Fragrance Journal, 27, no. 3 (2012):216-223,
https://doi.org/10.1002/ffj.3082 . .