TY  - JOUR
AU  - Popović, Marko
AU  - Tadić, Vojin
AU  - Mihailović, Marija
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6813
AB  - Insulin was discovered 100 years ago and has been well studied from the perspectives of life and biomedical sciences. This paper reports chemical and biothermodynamic properties of biosynthesis of insulin. This paper reports for the first time the molecular and empirical formulas, biosynthesis reactions, and thermodynamic properties of molecules and their biosynthesis for human preproinsulin, proinsulin, insulin chain A, insulin chain B, insulin, signal peptide and intermediate peptide (C-peptide). Based on these, metabolic reactions were formulated for conversion of preproinsulin to insulin and their thermodynamic feasibility was analyzed.
PB  - Taylor & Francis
T2  - Journal of Biomolecular Structure and Dynamics
T1  - From genotype to phenotype with biothermodynamics: Empirical formulas, biosynthesis reactions and thermodynamic properties of preproinsulin, proinsulin and insulin molecules
DO  - 10.1080/07391102.2023.2256880
ER  - 

@article{
author = "Popović, Marko and Tadić, Vojin and Mihailović, Marija",
year = "2023",
abstract = "Insulin was discovered 100 years ago and has been well studied from the perspectives of life and biomedical sciences. This paper reports chemical and biothermodynamic properties of biosynthesis of insulin. This paper reports for the first time the molecular and empirical formulas, biosynthesis reactions, and thermodynamic properties of molecules and their biosynthesis for human preproinsulin, proinsulin, insulin chain A, insulin chain B, insulin, signal peptide and intermediate peptide (C-peptide). Based on these, metabolic reactions were formulated for conversion of preproinsulin to insulin and their thermodynamic feasibility was analyzed.",
publisher = "Taylor & Francis",
journal = "Journal of Biomolecular Structure and Dynamics",
title = "From genotype to phenotype with biothermodynamics: Empirical formulas, biosynthesis reactions and thermodynamic properties of preproinsulin, proinsulin and insulin molecules",
doi = "10.1080/07391102.2023.2256880"
}

Popović, M., Tadić, V.,& Mihailović, M.. (2023). From genotype to phenotype with biothermodynamics: Empirical formulas, biosynthesis reactions and thermodynamic properties of preproinsulin, proinsulin and insulin molecules. in Journal of Biomolecular Structure and Dynamics
Taylor & Francis..
https://doi.org/10.1080/07391102.2023.2256880

Popović M, Tadić V, Mihailović M. From genotype to phenotype with biothermodynamics: Empirical formulas, biosynthesis reactions and thermodynamic properties of preproinsulin, proinsulin and insulin molecules. in Journal of Biomolecular Structure and Dynamics. 2023;.
doi:10.1080/07391102.2023.2256880 .

Popović, Marko, Tadić, Vojin, Mihailović, Marija, "From genotype to phenotype with biothermodynamics: Empirical formulas, biosynthesis reactions and thermodynamic properties of preproinsulin, proinsulin and insulin molecules" in Journal of Biomolecular Structure and Dynamics (2023),
https://doi.org/10.1080/07391102.2023.2256880 . .

TY  - JOUR
AU  - Panić, Stefan
AU  - Pantović Pavlović, Marijana
AU  - Varničić, Miroslava
AU  - Tadić, Vojin
AU  - Stopić, Srećko
AU  - Friedrich, Bernd
AU  - Pavlović, Miroslav M.
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5598
AB  - The main objective of this research was a systematic development of advanced micro/nanostructured materials based on the most used metal-oxides for ORR and metal-oxides with an extremely low-loading of Pt for comparison. Hybrid composites compared were: MnO2, La2O3, mixed lanthanum manganese oxides (LMO), and mixed lanthanum manganese oxides with reduced platinum load (LMO-Pt). The influence of the reduced amount of noble metal, as well as single oxide activity toward ORR, was analyzed. The complete electrochemical performance of the hybrid materials has been performed by means of CV, LSV, and EIS. It was shown that all synthesized catalytic materials were ORR-active with noticeable reduction currents in O2 saturated 0.1 M KOH. The ORR behavior indicated that the La2O3 electrode has a different mechanism than the other tested electrode materials (MnO2, LMO, and LMO-Pt). The EIS results have revealed that the ORR reaction is of a mixed character, being electrochemically and diffusion controlled. Even more, diffusion is of mixed character due to transport of O2 molecules and the chemical reaction of oxygen reduction. O2 diffusion was shown to be the dominant process for MnO2, LMO, and LMO-Pt electrolytic materials, while chemical reaction is the dominant process for La2O3 electrolytic materials.
PB  - MDPI AG
T2  - Catalysts
T1  - Rare-Earth/Manganese Oxide-Based Composites Materials for Electrochemical Oxygen Reduction Reaction
VL  - 12
IS  - 6
SP  - 641
DO  - 10.3390/catal12060641
ER  - 

@article{
author = "Panić, Stefan and Pantović Pavlović, Marijana and Varničić, Miroslava and Tadić, Vojin and Stopić, Srećko and Friedrich, Bernd and Pavlović, Miroslav M.",
year = "2022",
abstract = "The main objective of this research was a systematic development of advanced micro/nanostructured materials based on the most used metal-oxides for ORR and metal-oxides with an extremely low-loading of Pt for comparison. Hybrid composites compared were: MnO2, La2O3, mixed lanthanum manganese oxides (LMO), and mixed lanthanum manganese oxides with reduced platinum load (LMO-Pt). The influence of the reduced amount of noble metal, as well as single oxide activity toward ORR, was analyzed. The complete electrochemical performance of the hybrid materials has been performed by means of CV, LSV, and EIS. It was shown that all synthesized catalytic materials were ORR-active with noticeable reduction currents in O2 saturated 0.1 M KOH. The ORR behavior indicated that the La2O3 electrode has a different mechanism than the other tested electrode materials (MnO2, LMO, and LMO-Pt). The EIS results have revealed that the ORR reaction is of a mixed character, being electrochemically and diffusion controlled. Even more, diffusion is of mixed character due to transport of O2 molecules and the chemical reaction of oxygen reduction. O2 diffusion was shown to be the dominant process for MnO2, LMO, and LMO-Pt electrolytic materials, while chemical reaction is the dominant process for La2O3 electrolytic materials.",
publisher = "MDPI AG",
journal = "Catalysts",
title = "Rare-Earth/Manganese Oxide-Based Composites Materials for Electrochemical Oxygen Reduction Reaction",
volume = "12",
number = "6",
pages = "641",
doi = "10.3390/catal12060641"
}

Panić, S., Pantović Pavlović, M., Varničić, M., Tadić, V., Stopić, S., Friedrich, B.,& Pavlović, M. M.. (2022). Rare-Earth/Manganese Oxide-Based Composites Materials for Electrochemical Oxygen Reduction Reaction. in Catalysts
MDPI AG., 12(6), 641.
https://doi.org/10.3390/catal12060641

Panić S, Pantović Pavlović M, Varničić M, Tadić V, Stopić S, Friedrich B, Pavlović MM. Rare-Earth/Manganese Oxide-Based Composites Materials for Electrochemical Oxygen Reduction Reaction. in Catalysts. 2022;12(6):641.
doi:10.3390/catal12060641 .

Panić, Stefan, Pantović Pavlović, Marijana, Varničić, Miroslava, Tadić, Vojin, Stopić, Srećko, Friedrich, Bernd, Pavlović, Miroslav M., "Rare-Earth/Manganese Oxide-Based Composites Materials for Electrochemical Oxygen Reduction Reaction" in Catalysts, 12, no. 6 (2022):641,
https://doi.org/10.3390/catal12060641 . .

TY  - CONF
AU  - Panić, Stefan
AU  - Pantović Pavlović, Marijana
AU  - Božić, Katarina
AU  - Varničić, Miroslava
AU  - Stevanović, Maja
AU  - Tadić, Vojin
AU  - Pavlović, Miroslav
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6000
AB  - The main objective of this research is the development of catalytic materials based on a combination 
of metal-oxides with extremely low-loading of Pt for oxygen reduction reactions (ORR). Materials 
compared were La2O3 with 5 mass% loading of Pt (La2O3-Pt) and La2O3/MnO2 multioxide based 
material with 5 mass% loading of Pt (LMO-Pt). The electrochemical characterization of the catalytic 
materials has been done using cyclic voltametry (CV), linear sweap voltammetry (LSV) and 
galvanostatic electrochemical impedanse spectroscopy (GEIS). It was proven that both synthesized 
catalytic materials were ORR-active. The ORR behavior indicated that La2O3-Pt electrode is 
significantly less active than LMO-Pt. The GEIS results showed that the ORR is complex 
electrochemical reaction, being electrochemically and diffusion controlled. Diffusion is of mixed 
character due to transport of O2 molecule and chemical reaction of oxygen reduction reaction. O2
diffusion is the dominant process for LMO-Pt catalytic materials, while chemical reaction is 
dominant process for La2O3-Pt electrolytic material.
PB  - Beograd : Udruženje inženjera Srbije za koroziju i zaštitu materijala UISKOZAM
C3  - Proceedings - XXIII YuCorr International Conference, "Meeting point of the science and practice in the fields of corrosion, materials and environmental protection", May 16-19, 2022, Divčibare, Serbia / Knjiga radova - XXIII YuCorr Međunarodna konferencija, "Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine", 16-19 Maj, 2022, Divčibare
T1  - Rare-earth / manganese oxide-based composites for oxygen reduction reaction
SP  - poster 43
EP  - poster 47
UR  - https://hdl.handle.net/21.15107/rcub_cer_6000
ER  - 

@conference{
author = "Panić, Stefan and Pantović Pavlović, Marijana and Božić, Katarina and Varničić, Miroslava and Stevanović, Maja and Tadić, Vojin and Pavlović, Miroslav",
year = "2022",
abstract = "The main objective of this research is the development of catalytic materials based on a combination 
of metal-oxides with extremely low-loading of Pt for oxygen reduction reactions (ORR). Materials 
compared were La2O3 with 5 mass% loading of Pt (La2O3-Pt) and La2O3/MnO2 multioxide based 
material with 5 mass% loading of Pt (LMO-Pt). The electrochemical characterization of the catalytic 
materials has been done using cyclic voltametry (CV), linear sweap voltammetry (LSV) and 
galvanostatic electrochemical impedanse spectroscopy (GEIS). It was proven that both synthesized 
catalytic materials were ORR-active. The ORR behavior indicated that La2O3-Pt electrode is 
significantly less active than LMO-Pt. The GEIS results showed that the ORR is complex 
electrochemical reaction, being electrochemically and diffusion controlled. Diffusion is of mixed 
character due to transport of O2 molecule and chemical reaction of oxygen reduction reaction. O2
diffusion is the dominant process for LMO-Pt catalytic materials, while chemical reaction is 
dominant process for La2O3-Pt electrolytic material.",
publisher = "Beograd : Udruženje inženjera Srbije za koroziju i zaštitu materijala UISKOZAM",
journal = "Proceedings - XXIII YuCorr International Conference, "Meeting point of the science and practice in the fields of corrosion, materials and environmental protection", May 16-19, 2022, Divčibare, Serbia / Knjiga radova - XXIII YuCorr Međunarodna konferencija, "Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine", 16-19 Maj, 2022, Divčibare",
title = "Rare-earth / manganese oxide-based composites for oxygen reduction reaction",
pages = "poster 43-poster 47",
url = "https://hdl.handle.net/21.15107/rcub_cer_6000"
}

Panić, S., Pantović Pavlović, M., Božić, K., Varničić, M., Stevanović, M., Tadić, V.,& Pavlović, M.. (2022). Rare-earth / manganese oxide-based composites for oxygen reduction reaction. in Proceedings - XXIII YuCorr International Conference, "Meeting point of the science and practice in the fields of corrosion, materials and environmental protection", May 16-19, 2022, Divčibare, Serbia / Knjiga radova - XXIII YuCorr Međunarodna konferencija, "Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine", 16-19 Maj, 2022, Divčibare
Beograd : Udruženje inženjera Srbije za koroziju i zaštitu materijala UISKOZAM., poster 43-poster 47.
https://hdl.handle.net/21.15107/rcub_cer_6000

Panić S, Pantović Pavlović M, Božić K, Varničić M, Stevanović M, Tadić V, Pavlović M. Rare-earth / manganese oxide-based composites for oxygen reduction reaction. in Proceedings - XXIII YuCorr International Conference, "Meeting point of the science and practice in the fields of corrosion, materials and environmental protection", May 16-19, 2022, Divčibare, Serbia / Knjiga radova - XXIII YuCorr Međunarodna konferencija, "Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine", 16-19 Maj, 2022, Divčibare. 2022;:poster 43-poster 47.
https://hdl.handle.net/21.15107/rcub_cer_6000 .

Panić, Stefan, Pantović Pavlović, Marijana, Božić, Katarina, Varničić, Miroslava, Stevanović, Maja, Tadić, Vojin, Pavlović, Miroslav, "Rare-earth / manganese oxide-based composites for oxygen reduction reaction" in Proceedings - XXIII YuCorr International Conference, "Meeting point of the science and practice in the fields of corrosion, materials and environmental protection", May 16-19, 2022, Divčibare, Serbia / Knjiga radova - XXIII YuCorr Međunarodna konferencija, "Stecište nauke i prakse u oblastima korozije, zaštite materijala i životne sredine", 16-19 Maj, 2022, Divčibare (2022):poster 43-poster 47,
https://hdl.handle.net/21.15107/rcub_cer_6000 .

TY  - JOUR
AU  - Blažić, Marija
AU  - Balaž, Ana Marija
AU  - Tadić, Vojin
AU  - Draganić, Bojana
AU  - Ostafe, Raluca
AU  - Fischer, Rainer
AU  - Prodanović, Radivoje
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2655
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2656
AB  - Cellobiose dehydrogenase (CDH) can be used in industry for lactobionic acid production, as a part of biosensors for disaccharides and in wound healing. In fungi it is involved in lignocellulose degradation. CDH gene from Phanerochaete chrysosporium has been cloned in pYES2 plasmid for extracellular expression and protein engineering in yeast Saccharomyces cerevisiae InvSC1 for the first time. A CDH gene library was generated using error-prone PCR and screened by spectrophotometric enzymatic assaybased on 2,6-dichloroindophenol reduction detection in microtiter plates. Several mutants with increased activity and specificity towards lactose and cellobiose were found, purified and characterized in detail. Recombinant CDH enzymes showed a broad molecular weight between 120 and 150 KDa due to hyperglycosylation and the best S137N mutant showed 2.2 times increased kcat and 1.5 and 2 times increased specificity constant for lactose and cellobiose compared to the wild type enzyme. pH optimum of mutants was not changed while thermostability of selected mutants improved and S137N mutant retained 30% of it’s original activity after 15 minutes at 70oC compared to 10% of activity that the wild type enzyme retained. Mutants M65S and S137N showed also 1.6 and 1.5 times increased productivity of hydrogen peroxide in the presence of 30mM lactose compared to the wild type.
PB  - Elsevier
T2  - Biochemical Engineering Journal
T1  - Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability
VL  - 146
SP  - 179
EP  - 185
DO  - 10.1016/j.bej.2019.03.025
ER  - 

@article{
author = "Blažić, Marija and Balaž, Ana Marija and Tadić, Vojin and Draganić, Bojana and Ostafe, Raluca and Fischer, Rainer and Prodanović, Radivoje",
year = "2019",
abstract = "Cellobiose dehydrogenase (CDH) can be used in industry for lactobionic acid production, as a part of biosensors for disaccharides and in wound healing. In fungi it is involved in lignocellulose degradation. CDH gene from Phanerochaete chrysosporium has been cloned in pYES2 plasmid for extracellular expression and protein engineering in yeast Saccharomyces cerevisiae InvSC1 for the first time. A CDH gene library was generated using error-prone PCR and screened by spectrophotometric enzymatic assaybased on 2,6-dichloroindophenol reduction detection in microtiter plates. Several mutants with increased activity and specificity towards lactose and cellobiose were found, purified and characterized in detail. Recombinant CDH enzymes showed a broad molecular weight between 120 and 150 KDa due to hyperglycosylation and the best S137N mutant showed 2.2 times increased kcat and 1.5 and 2 times increased specificity constant for lactose and cellobiose compared to the wild type enzyme. pH optimum of mutants was not changed while thermostability of selected mutants improved and S137N mutant retained 30% of it’s original activity after 15 minutes at 70oC compared to 10% of activity that the wild type enzyme retained. Mutants M65S and S137N showed also 1.6 and 1.5 times increased productivity of hydrogen peroxide in the presence of 30mM lactose compared to the wild type.",
publisher = "Elsevier",
journal = "Biochemical Engineering Journal",
title = "Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability",
volume = "146",
pages = "179-185",
doi = "10.1016/j.bej.2019.03.025"
}

Blažić, M., Balaž, A. M., Tadić, V., Draganić, B., Ostafe, R., Fischer, R.,& Prodanović, R.. (2019). Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability. in Biochemical Engineering Journal
Elsevier., 146, 179-185.
https://doi.org/10.1016/j.bej.2019.03.025

Blažić M, Balaž AM, Tadić V, Draganić B, Ostafe R, Fischer R, Prodanović R. Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability. in Biochemical Engineering Journal. 2019;146:179-185.
doi:10.1016/j.bej.2019.03.025 .

Blažić, Marija, Balaž, Ana Marija, Tadić, Vojin, Draganić, Bojana, Ostafe, Raluca, Fischer, Rainer, Prodanović, Radivoje, "Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability" in Biochemical Engineering Journal, 146 (2019):179-185,
https://doi.org/10.1016/j.bej.2019.03.025 . .

TY  - JOUR
AU  - Blažić, Marija
AU  - Balaž, Ana Marija
AU  - Tadić, Vojin
AU  - Draganić, Bojana
AU  - Ostafe, Raluca
AU  - Fischer, Rainer
AU  - Prodanović, Radivoje
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2655
AB  - Cellobiose dehydrogenase (CDH) can be used in industry for lactobionic acid production, as a part of biosensors for disaccharides and in wound healing. In fungi it is involved in lignocellulose degradation. CDH gene from Phanerochaete chrysosporium has been cloned in pYES2 plasmid for extracellular expression and protein engineering in yeast Saccharomyces cerevisiae InvSC1 for the first time. A CDH gene library was generated using error-prone PCR and screened by spectrophotometric enzymatic assay
based on 2,6-dichloroindophenol reduction detection in microtiter plates. Several mutants with increased activity and specificity towards lactose and cellobiose were found, purified and characterized in detail. Recombinant CDH enzymes showed a broad molecular weight between 120 and 150 KDa due to hyperglycosylation and the best S137N mutant showed 2.2 times increased kcat and 1.5 and 2 times increased specificity constant for lactose and cellobiose compared to the wild type enzyme. pH optimum of mutants was not changed while thermostability of selected mutants improved and S137N mutant retained 30% of it’s original activity after 15 minutes at 70oC compared to 10% of activity that the wild type enzyme retained. Mutants M65S and S137N showed also 1.6 and 1.5 times increased productivity of hydrogen peroxide in the presence of 30mM lactose compared to the wild type.
PB  - Elsevier
T2  - Biochemical Engineering Journal
T1  - Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability
VL  - 146
SP  - 179
EP  - 185
DO  - 10.1016/j.bej.2019.03.025
ER  - 

@article{
author = "Blažić, Marija and Balaž, Ana Marija and Tadić, Vojin and Draganić, Bojana and Ostafe, Raluca and Fischer, Rainer and Prodanović, Radivoje",
year = "2019",
abstract = "Cellobiose dehydrogenase (CDH) can be used in industry for lactobionic acid production, as a part of biosensors for disaccharides and in wound healing. In fungi it is involved in lignocellulose degradation. CDH gene from Phanerochaete chrysosporium has been cloned in pYES2 plasmid for extracellular expression and protein engineering in yeast Saccharomyces cerevisiae InvSC1 for the first time. A CDH gene library was generated using error-prone PCR and screened by spectrophotometric enzymatic assay
based on 2,6-dichloroindophenol reduction detection in microtiter plates. Several mutants with increased activity and specificity towards lactose and cellobiose were found, purified and characterized in detail. Recombinant CDH enzymes showed a broad molecular weight between 120 and 150 KDa due to hyperglycosylation and the best S137N mutant showed 2.2 times increased kcat and 1.5 and 2 times increased specificity constant for lactose and cellobiose compared to the wild type enzyme. pH optimum of mutants was not changed while thermostability of selected mutants improved and S137N mutant retained 30% of it’s original activity after 15 minutes at 70oC compared to 10% of activity that the wild type enzyme retained. Mutants M65S and S137N showed also 1.6 and 1.5 times increased productivity of hydrogen peroxide in the presence of 30mM lactose compared to the wild type.",
publisher = "Elsevier",
journal = "Biochemical Engineering Journal",
title = "Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability",
volume = "146",
pages = "179-185",
doi = "10.1016/j.bej.2019.03.025"
}

Blažić, M., Balaž, A. M., Tadić, V., Draganić, B., Ostafe, R., Fischer, R.,& Prodanović, R.. (2019). Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability. in Biochemical Engineering Journal
Elsevier., 146, 179-185.
https://doi.org/10.1016/j.bej.2019.03.025

Blažić M, Balaž AM, Tadić V, Draganić B, Ostafe R, Fischer R, Prodanović R. Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability. in Biochemical Engineering Journal. 2019;146:179-185.
doi:10.1016/j.bej.2019.03.025 .

Blažić, Marija, Balaž, Ana Marija, Tadić, Vojin, Draganić, Bojana, Ostafe, Raluca, Fischer, Rainer, Prodanović, Radivoje, "Protein engineering of cellobiose dehydrogenase from Phanerochaete chrysosporium in yeast Saccharomyces cerevisiae InvSc1 for increased activity and stability" in Biochemical Engineering Journal, 146 (2019):179-185,
https://doi.org/10.1016/j.bej.2019.03.025 . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Petrić, Marija
AU  - Uzelac, Branka
AU  - Milosevic, Snezana
AU  - Vujčić, Zoran
AU  - Stevanović, Jasmina
AU  - Tadić, Jovan
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2321
AB  - We investigated the removal of phenol from water solutions (200 mg L-1) using two varieties of lettuce (Lactuca sativa L) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L-1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L-1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L-1, but were not able to remove phenol at constant concentration above 25 mg L-1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.
PB  - Elsevier
T2  - Scientia Horticulturae
T1  - Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1
VL  - 231
SP  - 210
EP  - 218
DO  - 10.1016/j.scienta.2017.12.025
ER  - 

@article{
author = "Tadić, Vojin and Petrić, Marija and Uzelac, Branka and Milosevic, Snezana and Vujčić, Zoran and Stevanović, Jasmina and Tadić, Jovan",
year = "2018",
abstract = "We investigated the removal of phenol from water solutions (200 mg L-1) using two varieties of lettuce (Lactuca sativa L) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L-1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L-1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L-1, but were not able to remove phenol at constant concentration above 25 mg L-1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.",
publisher = "Elsevier",
journal = "Scientia Horticulturae",
title = "Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1",
volume = "231",
pages = "210-218",
doi = "10.1016/j.scienta.2017.12.025"
}

Tadić, V., Petrić, M., Uzelac, B., Milosevic, S., Vujčić, Z., Stevanović, J.,& Tadić, J.. (2018). Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1. in Scientia Horticulturae
Elsevier., 231, 210-218.
https://doi.org/10.1016/j.scienta.2017.12.025

Tadić V, Petrić M, Uzelac B, Milosevic S, Vujčić Z, Stevanović J, Tadić J. Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1. in Scientia Horticulturae. 2018;231:210-218.
doi:10.1016/j.scienta.2017.12.025 .

Tadić, Vojin, Petrić, Marija, Uzelac, Branka, Milosevic, Snezana, Vujčić, Zoran, Stevanović, Jasmina, Tadić, Jovan, "Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1" in Scientia Horticulturae, 231 (2018):210-218,
https://doi.org/10.1016/j.scienta.2017.12.025 . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Balaž, Ana Marija
AU  - Petrić, Marija
AU  - Milošević, Snežana M.
AU  - Zelenović, Nevena
AU  - Raspor, Martin Z.
AU  - Tadić, Jovan
AU  - Prodanović, Radivoje
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1644
AB  - We have cloned the gene for carbohydrate oxidase (CHO) from Lactuca sativa in two species of yeasts (Saccharomyces cerevisiae and Pichia pastoris). The synthetic gene Department for Plant Physiology, Institute for Biological Research 'Siniša Stanković', drate oxidase (1821 bp) from L. sativa cloned into the vector pUC57 and inserted into plasmids pYES2 and pGAP using Escherichia coli DH5α strain. The P. pastoris strain X-33 and the S. cerevisiae strain InvSC1 were used for extracellular expression of CHO. After transformation of P. pastoris X-33 with CHO-pGAP construct none of the colonies showed CHO activity. Two samples displayed a band which did not exist in the sample with the empty vector similar to the molecular weight of CHO. The S. cerevisiae stran InvSC1 has been also transformed with CHO-pYES constructs. Three colonies grew on the plate with cells transformed with the construct. One of the samples showed a band corresponding to about 110 kDa, but no CHO activity was recorded in this case either. Cloning of the foreign genes and heterologous expression in yeasts is widely used in biotechnology, but sometimes can be very dependent on the gene sequence and strain used. In order to obtain active CHO enzyme the further studies on purification and refolding of expressed protein are necessary.
AB  - Ugljeni hidrat-oksidaza (CHO) iz zelene salate (Lactuca sativa) je enzim koji je do danas nedovoljno ispitan. Vrlo se malo zna o njegovoj strukturi i funkciji. CHO pripada velikoj familiji ugljenihidrat-oksidaza, koje oksiduju šećere. Svaki od članova ove velike familije dobio je ime po supstratu koji oksiduje. Oksidaze iz ove familije enzima imaju kako sličnu ulogu tako i sličnu strukutru. Sve ili većina ovih enzima su monomeri, čiji se polipeptidni lanac uvija u dva domena. Jedan od domena vezuje flavinski kofaktor, a drugi domen je supstrat vezujući. Većina njih oksidaciju supstrata vrši po takozvanom ping-pong mehanizmu. Sve oksidaze iz karbohidrat-oksidaza familije, pa među njima i enzim koji je predmet ove studije (CHO), danas su našle veliku primenu u industriji. CHO se može primenjivati kako u medicinskoj djagnostici, konkretno u biosenzorima za određivanje glukoze u krvi, u prehrambenoj industriji, poljoprivredi, proizvodnji hleba, deterdženata i u raznim drugim industrijskim oblastima. Problem sa ovim enzimom, kao i sa ostalim članovima ove familije, jeste niska koncentracija u prirodnim izvorima. Zato su danas razvijene različite metode rekombinantne tehnologije, kojima se dobijaju ovi enzimi. U ovom radu opisano je kloniranje gena za CHO iz zelene salate u dve vrste kvasaca (Saccharomyces cerevisiae i Pichia pastoris). Sintetički gen za CHO (1821 bp) iz zelene salate kloniran je u vektor pUC57. Escherichia coli soj DH5α korišćen je za kloniranje gena i održavanje plazmida. P. pastoris soj X-33 i S. cerevisiae soj InvSC1 korišćeni su za ekstracelularnu ekspresiju CHO. Aktivnost CHO određena je ABST esejom, a promena absorbance merena je na 405 nm. Potvrda prisustva enzima rađena je na DNK agaroznoj elektroforezi i SDS-PAGE. Posle transformacije P. pastoris X-33, nijedan od klonova nije pokazivao aktivnost CHO. Posle prve fermentacije, kolonije su testirane na SDS-PAGE. Kako su dva uzorka pokazala trake, koje ne postoje na praznom vektoru, ove trake bi mogle odgovarati željenom enzimu, CHO. Traka se nalazi na molekulskoj masi koja je veća od teoretske (više od 120 kDa). Enzim bi mogao biti glikolizovan i zbog toga pokazivati ovako velike vrednosti za molekulsku masu. S. cerevisiae soj InvSC1 transformisan je konstruktom CHO-pZES. Posle 24 sata, tri kolonije su porasle na ploči na kojoj su bile ćelije transformisane pomenutim konstruktom. Uzorci su testirani na SDS-PAGE. Jedan uzorak je pokazao traku na oko 110 kDa, ali aktivnost CHO nije potvrđena takođe. Cilj ove studije je bio kloniranje CHO u kvascima S. cerevisiae i P. pastoris, kao i njena ekspresija u ovim, danas široko primenjivanim ekspresionim sistemima.
PB  - Assoc Chemical Engineers Serbia, Belgrade
T2  - Hemijska industrija
T1  - Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in the yeasts Saccharomyces cerevisiae and Pichia pastoris
T1  - Kloniranje gena za ugljeni hidrat oksidazu iz biljke Lactuca sativa u kvasce saccharomyces cerevisiae i Pichia pastoris
VL  - 69
IS  - 6
SP  - 689
EP  - 701
DO  - 10.2298/HEMIND140823003T
ER  - 

@article{
author = "Tadić, Vojin and Balaž, Ana Marija and Petrić, Marija and Milošević, Snežana M. and Zelenović, Nevena and Raspor, Martin Z. and Tadić, Jovan and Prodanović, Radivoje",
year = "2015",
abstract = "We have cloned the gene for carbohydrate oxidase (CHO) from Lactuca sativa in two species of yeasts (Saccharomyces cerevisiae and Pichia pastoris). The synthetic gene Department for Plant Physiology, Institute for Biological Research 'Siniša Stanković', drate oxidase (1821 bp) from L. sativa cloned into the vector pUC57 and inserted into plasmids pYES2 and pGAP using Escherichia coli DH5α strain. The P. pastoris strain X-33 and the S. cerevisiae strain InvSC1 were used for extracellular expression of CHO. After transformation of P. pastoris X-33 with CHO-pGAP construct none of the colonies showed CHO activity. Two samples displayed a band which did not exist in the sample with the empty vector similar to the molecular weight of CHO. The S. cerevisiae stran InvSC1 has been also transformed with CHO-pYES constructs. Three colonies grew on the plate with cells transformed with the construct. One of the samples showed a band corresponding to about 110 kDa, but no CHO activity was recorded in this case either. Cloning of the foreign genes and heterologous expression in yeasts is widely used in biotechnology, but sometimes can be very dependent on the gene sequence and strain used. In order to obtain active CHO enzyme the further studies on purification and refolding of expressed protein are necessary., Ugljeni hidrat-oksidaza (CHO) iz zelene salate (Lactuca sativa) je enzim koji je do danas nedovoljno ispitan. Vrlo se malo zna o njegovoj strukturi i funkciji. CHO pripada velikoj familiji ugljenihidrat-oksidaza, koje oksiduju šećere. Svaki od članova ove velike familije dobio je ime po supstratu koji oksiduje. Oksidaze iz ove familije enzima imaju kako sličnu ulogu tako i sličnu strukutru. Sve ili većina ovih enzima su monomeri, čiji se polipeptidni lanac uvija u dva domena. Jedan od domena vezuje flavinski kofaktor, a drugi domen je supstrat vezujući. Većina njih oksidaciju supstrata vrši po takozvanom ping-pong mehanizmu. Sve oksidaze iz karbohidrat-oksidaza familije, pa među njima i enzim koji je predmet ove studije (CHO), danas su našle veliku primenu u industriji. CHO se može primenjivati kako u medicinskoj djagnostici, konkretno u biosenzorima za određivanje glukoze u krvi, u prehrambenoj industriji, poljoprivredi, proizvodnji hleba, deterdženata i u raznim drugim industrijskim oblastima. Problem sa ovim enzimom, kao i sa ostalim članovima ove familije, jeste niska koncentracija u prirodnim izvorima. Zato su danas razvijene različite metode rekombinantne tehnologije, kojima se dobijaju ovi enzimi. U ovom radu opisano je kloniranje gena za CHO iz zelene salate u dve vrste kvasaca (Saccharomyces cerevisiae i Pichia pastoris). Sintetički gen za CHO (1821 bp) iz zelene salate kloniran je u vektor pUC57. Escherichia coli soj DH5α korišćen je za kloniranje gena i održavanje plazmida. P. pastoris soj X-33 i S. cerevisiae soj InvSC1 korišćeni su za ekstracelularnu ekspresiju CHO. Aktivnost CHO određena je ABST esejom, a promena absorbance merena je na 405 nm. Potvrda prisustva enzima rađena je na DNK agaroznoj elektroforezi i SDS-PAGE. Posle transformacije P. pastoris X-33, nijedan od klonova nije pokazivao aktivnost CHO. Posle prve fermentacije, kolonije su testirane na SDS-PAGE. Kako su dva uzorka pokazala trake, koje ne postoje na praznom vektoru, ove trake bi mogle odgovarati željenom enzimu, CHO. Traka se nalazi na molekulskoj masi koja je veća od teoretske (više od 120 kDa). Enzim bi mogao biti glikolizovan i zbog toga pokazivati ovako velike vrednosti za molekulsku masu. S. cerevisiae soj InvSC1 transformisan je konstruktom CHO-pZES. Posle 24 sata, tri kolonije su porasle na ploči na kojoj su bile ćelije transformisane pomenutim konstruktom. Uzorci su testirani na SDS-PAGE. Jedan uzorak je pokazao traku na oko 110 kDa, ali aktivnost CHO nije potvrđena takođe. Cilj ove studije je bio kloniranje CHO u kvascima S. cerevisiae i P. pastoris, kao i njena ekspresija u ovim, danas široko primenjivanim ekspresionim sistemima.",
publisher = "Assoc Chemical Engineers Serbia, Belgrade",
journal = "Hemijska industrija",
title = "Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in the yeasts Saccharomyces cerevisiae and Pichia pastoris, Kloniranje gena za ugljeni hidrat oksidazu iz biljke Lactuca sativa u kvasce saccharomyces cerevisiae i Pichia pastoris",
volume = "69",
number = "6",
pages = "689-701",
doi = "10.2298/HEMIND140823003T"
}

Tadić, V., Balaž, A. M., Petrić, M., Milošević, S. M., Zelenović, N., Raspor, M. Z., Tadić, J.,& Prodanović, R.. (2015). Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in the yeasts Saccharomyces cerevisiae and Pichia pastoris. in Hemijska industrija
Assoc Chemical Engineers Serbia, Belgrade., 69(6), 689-701.
https://doi.org/10.2298/HEMIND140823003T

Tadić V, Balaž AM, Petrić M, Milošević SM, Zelenović N, Raspor MZ, Tadić J, Prodanović R. Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in the yeasts Saccharomyces cerevisiae and Pichia pastoris. in Hemijska industrija. 2015;69(6):689-701.
doi:10.2298/HEMIND140823003T .

Tadić, Vojin, Balaž, Ana Marija, Petrić, Marija, Milošević, Snežana M., Zelenović, Nevena, Raspor, Martin Z., Tadić, Jovan, Prodanović, Radivoje, "Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in the yeasts Saccharomyces cerevisiae and Pichia pastoris" in Hemijska industrija, 69, no. 6 (2015):689-701,
https://doi.org/10.2298/HEMIND140823003T . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Aljančić, Ivana
AU  - Vajs, Vlatka
AU  - Milosavljević, Slobodan
AU  - Đoković, Dejan
AU  - Đorđević, Iris
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3265
AB  - The essential oil from aerial parts of Tanacetum larvatum (gris.) Kanitz, an endemic perennial herb collected at three various natural habitats in Montenegro (Planinica [Sample a ], Visitor [Sample b ] and Sinjajevina [Sample c ]), were analyzed using GC and GC/MS. Sixty-four components were identified, representing 83.1%, 96.6% and 89.4% of the total oils content in the Samples a, b and c , respectively. The major constituent in Samples a and b , was oxygenated monoterpene, trans-sabinyl acetate (38.1% and 55.8% respectively). Monoterpene hydrocarbons, β-pinene (13.5%) and santolinatriene (30.6%), were found to be the dominant components in Sample c . The toxic trans-sabinyl acetate was present only in traces in this sample. trans-Chrysanthenyl acetate, as one of major components in feverfew essential oil, has not been previously identified in the investigated essential oils.
PB  - Informa UK Limited
T2  - Journal of Essential Oil Research
T1  - Intraspecific Variation of Tanacetum larvatum Essential Oil
VL  - 22
IS  - 5
SP  - 394
EP  - 398
DO  - 10.1080/10412905.2010.9700355
ER  - 

@article{
author = "Tadić, Vojin and Aljančić, Ivana and Vajs, Vlatka and Milosavljević, Slobodan and Đoković, Dejan and Đorđević, Iris",
year = "2010",
abstract = "The essential oil from aerial parts of Tanacetum larvatum (gris.) Kanitz, an endemic perennial herb collected at three various natural habitats in Montenegro (Planinica [Sample a ], Visitor [Sample b ] and Sinjajevina [Sample c ]), were analyzed using GC and GC/MS. Sixty-four components were identified, representing 83.1%, 96.6% and 89.4% of the total oils content in the Samples a, b and c , respectively. The major constituent in Samples a and b , was oxygenated monoterpene, trans-sabinyl acetate (38.1% and 55.8% respectively). Monoterpene hydrocarbons, β-pinene (13.5%) and santolinatriene (30.6%), were found to be the dominant components in Sample c . The toxic trans-sabinyl acetate was present only in traces in this sample. trans-Chrysanthenyl acetate, as one of major components in feverfew essential oil, has not been previously identified in the investigated essential oils.",
publisher = "Informa UK Limited",
journal = "Journal of Essential Oil Research",
title = "Intraspecific Variation of Tanacetum larvatum Essential Oil",
volume = "22",
number = "5",
pages = "394-398",
doi = "10.1080/10412905.2010.9700355"
}

Tadić, V., Aljančić, I., Vajs, V., Milosavljević, S., Đoković, D.,& Đorđević, I.. (2010). Intraspecific Variation of Tanacetum larvatum Essential Oil. in Journal of Essential Oil Research
Informa UK Limited., 22(5), 394-398.
https://doi.org/10.1080/10412905.2010.9700355

Tadić V, Aljančić I, Vajs V, Milosavljević S, Đoković D, Đorđević I. Intraspecific Variation of Tanacetum larvatum Essential Oil. in Journal of Essential Oil Research. 2010;22(5):394-398.
doi:10.1080/10412905.2010.9700355 .

Tadić, Vojin, Aljančić, Ivana, Vajs, Vlatka, Milosavljević, Slobodan, Đoković, Dejan, Đorđević, Iris, "Intraspecific Variation of Tanacetum larvatum Essential Oil" in Journal of Essential Oil Research, 22, no. 5 (2010):394-398,
https://doi.org/10.1080/10412905.2010.9700355 . .