TY  - JOUR
AU  - Ramadan, Muftah Mohamed Ali
AU  - Šolević Knudsen, Tatjana
AU  - Antić, Mališa
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav
AU  - Schwarzbauer, Jan
AU  - Jovančićević, Branimir
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1208
AB  - It is well known that during biodegradation of oil under natural geological conditions, or oil pollutants in the environment, degradation of hydrocarbons occurs according to a well-defined sequence. For example, the major changes during the degradation process of n-alkanes occur in the second, slight and third, moderate level (on the biodegradation scale from 1 to 10). According to previous research, in the fourth, heavy level, when intensive changes of phenanthrene and its methyl isomers begin, n-alkanes have already been completely removed. In this paper, the ex situ natural bioremediation (non-stimulated bioremediation, without addition of biomass, nutrient substances and biosurfactant) of soil contaminated with heavy residual fuel oil (mazut) was conducted during a period of 6 months. Low abundance of n-alkanes in the fraction of total saturated hydrocarbons in the initial sample (identification was possible only after concentration by the urea adduction technique) showed that the investigated oil pollutant was at the boundary between the third and the fourth biodegradation level. During the experiment, an intense degradation of phenanthrene and its methyl-, dimethyl- and tri-methyl-isomers was not accompanied by the removal of the remaining n-alkanes. The abundance of n-alkanes remained at the initial low level, even at end of the experiment when the pollutant reached one of the highest biodegradation levels. These results showed that the non-stimulated biodegradation of some hydrocarbons, despite their high biodegradability, had not proceeded completely to the end, even at final degradation stages. Under the condition of reduced availability of some hydrocarbons, microorganisms tend to opt for the less biodegradable but more accessible hydrocarbons.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut)
VL  - 78
IS  - 7
SP  - 1035
EP  - 1043
DO  - 10.2298/JSC120829106A
ER  - 

@article{
author = "Ramadan, Muftah Mohamed Ali and Šolević Knudsen, Tatjana and Antić, Mališa and Beškoski, Vladimir and Vrvić, Miroslav and Schwarzbauer, Jan and Jovančićević, Branimir",
year = "2013",
abstract = "It is well known that during biodegradation of oil under natural geological conditions, or oil pollutants in the environment, degradation of hydrocarbons occurs according to a well-defined sequence. For example, the major changes during the degradation process of n-alkanes occur in the second, slight and third, moderate level (on the biodegradation scale from 1 to 10). According to previous research, in the fourth, heavy level, when intensive changes of phenanthrene and its methyl isomers begin, n-alkanes have already been completely removed. In this paper, the ex situ natural bioremediation (non-stimulated bioremediation, without addition of biomass, nutrient substances and biosurfactant) of soil contaminated with heavy residual fuel oil (mazut) was conducted during a period of 6 months. Low abundance of n-alkanes in the fraction of total saturated hydrocarbons in the initial sample (identification was possible only after concentration by the urea adduction technique) showed that the investigated oil pollutant was at the boundary between the third and the fourth biodegradation level. During the experiment, an intense degradation of phenanthrene and its methyl-, dimethyl- and tri-methyl-isomers was not accompanied by the removal of the remaining n-alkanes. The abundance of n-alkanes remained at the initial low level, even at end of the experiment when the pollutant reached one of the highest biodegradation levels. These results showed that the non-stimulated biodegradation of some hydrocarbons, despite their high biodegradability, had not proceeded completely to the end, even at final degradation stages. Under the condition of reduced availability of some hydrocarbons, microorganisms tend to opt for the less biodegradable but more accessible hydrocarbons.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut)",
volume = "78",
number = "7",
pages = "1035-1043",
doi = "10.2298/JSC120829106A"
}

Ramadan, M. M. A., Šolević Knudsen, T., Antić, M., Beškoski, V., Vrvić, M., Schwarzbauer, J.,& Jovančićević, B.. (2013). Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut). in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 78(7), 1035-1043.
https://doi.org/10.2298/JSC120829106A

Ramadan MMA, Šolević Knudsen T, Antić M, Beškoski V, Vrvić M, Schwarzbauer J, Jovančićević B. Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut). in Journal of the Serbian Chemical Society. 2013;78(7):1035-1043.
doi:10.2298/JSC120829106A .

Ramadan, Muftah Mohamed Ali, Šolević Knudsen, Tatjana, Antić, Mališa, Beškoski, Vladimir, Vrvić, Miroslav, Schwarzbauer, Jan, Jovančićević, Branimir, "Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut)" in Journal of the Serbian Chemical Society, 78, no. 7 (2013):1035-1043,
https://doi.org/10.2298/JSC120829106A . .

TY  - JOUR
AU  - Novakovic, Milan
AU  - Ramadan, Muftah Mohamed Ali
AU  - Šolević Knudsen, Tatjana
AU  - Antić, Mališa
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
AU  - Jovančićević, Branimir
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1116
AB  - Phenanthrene and methyl-phenanthrenes are major aromatic pollutants originating in particular from fuel oil. Phenanthrene is usually degraded faster than methyl-phenanthrenes under geological and environmental conditions. Here, we report a preferential and accelerated biodegradation of methyl-phenanthrenes versus phenanthrene in soil contaminated by fuel oil. The polluted soil was mixed with sawdust and sand to form a homogenized biopile. The biopile was continuously sprayed with microbial consortia isolated from crude oil-contaminated soil and treated by biosurfactants and nutritive substances for biostimulation. During a 6-month bioremediation experiment, a steady increase in the relative abundance of phenanthrene compared to methyl-phenathrenes was observed by gas chromatography-mass spectrometry. The increase was the highest for trimethyl-phenanthrenes, with a phenanthrene/trimethyl-phenanthrenes ratio increasing from 0.42 to 2.45. By contrast, the control, non-stimulated samples showed a ratio decrease from 0.85 to 0.11. Moreover, the results showed that the level of degradability depends on the number of methyl groups.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Chemistry Letters
T1  - Degradation of methyl-phenanthrene isomers during bioremediation of soil contaminated by residual fuel oil
VL  - 10
IS  - 3
SP  - 287
EP  - 294
DO  - 10.1007/s10311-012-0354-6
ER  - 

@article{
author = "Novakovic, Milan and Ramadan, Muftah Mohamed Ali and Šolević Knudsen, Tatjana and Antić, Mališa and Beškoski, Vladimir and Gojgić-Cvijović, Gordana and Vrvić, Miroslav and Jovančićević, Branimir",
year = "2012",
abstract = "Phenanthrene and methyl-phenanthrenes are major aromatic pollutants originating in particular from fuel oil. Phenanthrene is usually degraded faster than methyl-phenanthrenes under geological and environmental conditions. Here, we report a preferential and accelerated biodegradation of methyl-phenanthrenes versus phenanthrene in soil contaminated by fuel oil. The polluted soil was mixed with sawdust and sand to form a homogenized biopile. The biopile was continuously sprayed with microbial consortia isolated from crude oil-contaminated soil and treated by biosurfactants and nutritive substances for biostimulation. During a 6-month bioremediation experiment, a steady increase in the relative abundance of phenanthrene compared to methyl-phenathrenes was observed by gas chromatography-mass spectrometry. The increase was the highest for trimethyl-phenanthrenes, with a phenanthrene/trimethyl-phenanthrenes ratio increasing from 0.42 to 2.45. By contrast, the control, non-stimulated samples showed a ratio decrease from 0.85 to 0.11. Moreover, the results showed that the level of degradability depends on the number of methyl groups.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Chemistry Letters",
title = "Degradation of methyl-phenanthrene isomers during bioremediation of soil contaminated by residual fuel oil",
volume = "10",
number = "3",
pages = "287-294",
doi = "10.1007/s10311-012-0354-6"
}

Novakovic, M., Ramadan, M. M. A., Šolević Knudsen, T., Antić, M., Beškoski, V., Gojgić-Cvijović, G., Vrvić, M.,& Jovančićević, B.. (2012). Degradation of methyl-phenanthrene isomers during bioremediation of soil contaminated by residual fuel oil. in Environmental Chemistry Letters
Springer Heidelberg, Heidelberg., 10(3), 287-294.
https://doi.org/10.1007/s10311-012-0354-6

Novakovic M, Ramadan MMA, Šolević Knudsen T, Antić M, Beškoski V, Gojgić-Cvijović G, Vrvić M, Jovančićević B. Degradation of methyl-phenanthrene isomers during bioremediation of soil contaminated by residual fuel oil. in Environmental Chemistry Letters. 2012;10(3):287-294.
doi:10.1007/s10311-012-0354-6 .

Novakovic, Milan, Ramadan, Muftah Mohamed Ali, Šolević Knudsen, Tatjana, Antić, Mališa, Beškoski, Vladimir, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, Jovančićević, Branimir, "Degradation of methyl-phenanthrene isomers during bioremediation of soil contaminated by residual fuel oil" in Environmental Chemistry Letters, 10, no. 3 (2012):287-294,
https://doi.org/10.1007/s10311-012-0354-6 . .