TY  - JOUR
AU  - Pavlović, Ratko
AU  - Dojnov, Biljana
AU  - Šokarda Slavić, Marinela
AU  - Pavlović, Marija
AU  - Slomo, Katarina
AU  - Ristović, Marina
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5593
AB  - Finding a pollen substitute for honey bees that is nutritionally adequate and affordable is a scientific and practical challenge. We attempted a new rational approach and tried to exploit honey bees’ natural cannibalistic behavior. We tested processed insect larvae as a food source that is nutritionally similar to bee brood, and which can easily be produced on a large scale. In cage experiments, monitoring bee mortality, food consumption and changes in bee body parts’ weights showed that flour obtained by grinding dried yellow mealworm larvae has the potential to become an excellent component for pollen substitution. Bees from the cage group fed Tenebrio molitor patties (TG) demonstrated overall best results in comparison to sugar patties fed bee group (CG), yeast patties fed bee group (YG) and pollen patties fed bee group (PG). They did not lose weight as rapidly as the CG, did not defecate inside cages as the YG, nor show increased mortality as the PG. At the same time, TG consumed less food (mean 13.7 g/cage) than CG (16.8 g/cage), YG (20.4 g/cage) and PG (23.9 g/cage) within the period of 28 days. Bees’ gut increase in weight was lowest in the CG, followed by TG and PG and was the highest in the YG which resulted in diarrhea after 14 days. Bees from TG did not lag behind other bees in head, thorax and abdomen weight after 28 days. We demonstrated that processed yellow mealworm larvae (T. molitor) can be used as an ingredient for honey bee feed.
PB  - Informa UK Limited
T2  - Journal of Apicultural Research
T1  - In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed
VL  - 62
IS  - 5
SP  - 1007
EP  - 1016
DO  - 10.1080/00218839.2022.2080950
ER  - 

@article{
author = "Pavlović, Ratko and Dojnov, Biljana and Šokarda Slavić, Marinela and Pavlović, Marija and Slomo, Katarina and Ristović, Marina and Vujčić, Zoran",
year = "2023",
abstract = "Finding a pollen substitute for honey bees that is nutritionally adequate and affordable is a scientific and practical challenge. We attempted a new rational approach and tried to exploit honey bees’ natural cannibalistic behavior. We tested processed insect larvae as a food source that is nutritionally similar to bee brood, and which can easily be produced on a large scale. In cage experiments, monitoring bee mortality, food consumption and changes in bee body parts’ weights showed that flour obtained by grinding dried yellow mealworm larvae has the potential to become an excellent component for pollen substitution. Bees from the cage group fed Tenebrio molitor patties (TG) demonstrated overall best results in comparison to sugar patties fed bee group (CG), yeast patties fed bee group (YG) and pollen patties fed bee group (PG). They did not lose weight as rapidly as the CG, did not defecate inside cages as the YG, nor show increased mortality as the PG. At the same time, TG consumed less food (mean 13.7 g/cage) than CG (16.8 g/cage), YG (20.4 g/cage) and PG (23.9 g/cage) within the period of 28 days. Bees’ gut increase in weight was lowest in the CG, followed by TG and PG and was the highest in the YG which resulted in diarrhea after 14 days. Bees from TG did not lag behind other bees in head, thorax and abdomen weight after 28 days. We demonstrated that processed yellow mealworm larvae (T. molitor) can be used as an ingredient for honey bee feed.",
publisher = "Informa UK Limited",
journal = "Journal of Apicultural Research",
title = "In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed",
volume = "62",
number = "5",
pages = "1007-1016",
doi = "10.1080/00218839.2022.2080950"
}

Pavlović, R., Dojnov, B., Šokarda Slavić, M., Pavlović, M., Slomo, K., Ristović, M.,& Vujčić, Z.. (2023). In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed. in Journal of Apicultural Research
Informa UK Limited., 62(5), 1007-1016.
https://doi.org/10.1080/00218839.2022.2080950

Pavlović R, Dojnov B, Šokarda Slavić M, Pavlović M, Slomo K, Ristović M, Vujčić Z. In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed. in Journal of Apicultural Research. 2023;62(5):1007-1016.
doi:10.1080/00218839.2022.2080950 .

Pavlović, Ratko, Dojnov, Biljana, Šokarda Slavić, Marinela, Pavlović, Marija, Slomo, Katarina, Ristović, Marina, Vujčić, Zoran, "In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed" in Journal of Apicultural Research, 62, no. 5 (2023):1007-1016,
https://doi.org/10.1080/00218839.2022.2080950 . .

TY  - CONF
AU  - Pavlović, Ratko
AU  - Dojnov, Biljana
AU  - Šokarda Slavić, Marinela
AU  - Pavlović, Marija
AU  - Slomo, Katarina
AU  - Ristović, Marina
AU  - Vujčić, Zoran
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5886
AB  - Fed of honey bee (Apis mellifera) is challenging for beekeepers and formulation of supplemental food is improved continuously. When natural food sources are scarce or not available, supplemental foods are widely used to support and build up honey bee colonies. Influence of commercial patty and patty enriched with 12.5% pollen on amylase expression in honey bee adults is presented. This
is part of a wider study aimed to compare the activity of digestive enzymes when using different patties. We assume that data collected in this way can be used for the development of better food supplements for honey bees.
Honey bees were kept in an incubator for 21 days, at a temperature of 35°C and at 80% humidity. In each cage, there were one hundred bees and a piece of honeycomb. Midgut and hindgut samples were taken after 7, 14 and 21 days and midgut without hindgut was taken after 21 days. Samples were homogenized and used for amylase zymogram, IEF and enzyme assays.
There was no mortality during the experiment. A high protein concentration was detected in the midgut in both groups of bees. Amylase activity was significantly higher in bees fed pollen enriched patties, which is shown by enzyme assay and by zymograms. There are different amylase isoforms present in bees fed by pollen enriched patties in comparison to bees fed by commercial patties, but
the major isoforms were the same.
The observed decrease in the amylase activity over time is probably due to dilution caused by the accumulation of water and undigested substances in the hindgut. Reduced amylase activity in the intestines of bees fed by commercial patties is due to a lack of starch or some other inducers present in pollen.
Because pollen is honey bee natural food, we conclude that food supplements that induce similar enzyme expression as pollen can be superior in comparison to supplements that induce very different enzyme expression.
PB  - Belgrade : University of Belgrade
C3  - Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade, Serbia
T1  - Expression of amylases in adult honey bees fed with different patties
SP  - 196
UR  - https://hdl.handle.net/21.15107/rcub_cer_5886
ER  - 

@conference{
author = "Pavlović, Ratko and Dojnov, Biljana and Šokarda Slavić, Marinela and Pavlović, Marija and Slomo, Katarina and Ristović, Marina and Vujčić, Zoran",
year = "2021",
abstract = "Fed of honey bee (Apis mellifera) is challenging for beekeepers and formulation of supplemental food is improved continuously. When natural food sources are scarce or not available, supplemental foods are widely used to support and build up honey bee colonies. Influence of commercial patty and patty enriched with 12.5% pollen on amylase expression in honey bee adults is presented. This
is part of a wider study aimed to compare the activity of digestive enzymes when using different patties. We assume that data collected in this way can be used for the development of better food supplements for honey bees.
Honey bees were kept in an incubator for 21 days, at a temperature of 35°C and at 80% humidity. In each cage, there were one hundred bees and a piece of honeycomb. Midgut and hindgut samples were taken after 7, 14 and 21 days and midgut without hindgut was taken after 21 days. Samples were homogenized and used for amylase zymogram, IEF and enzyme assays.
There was no mortality during the experiment. A high protein concentration was detected in the midgut in both groups of bees. Amylase activity was significantly higher in bees fed pollen enriched patties, which is shown by enzyme assay and by zymograms. There are different amylase isoforms present in bees fed by pollen enriched patties in comparison to bees fed by commercial patties, but
the major isoforms were the same.
The observed decrease in the amylase activity over time is probably due to dilution caused by the accumulation of water and undigested substances in the hindgut. Reduced amylase activity in the intestines of bees fed by commercial patties is due to a lack of starch or some other inducers present in pollen.
Because pollen is honey bee natural food, we conclude that food supplements that induce similar enzyme expression as pollen can be superior in comparison to supplements that induce very different enzyme expression.",
publisher = "Belgrade : University of Belgrade",
journal = "Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade, Serbia",
title = "Expression of amylases in adult honey bees fed with different patties",
pages = "196",
url = "https://hdl.handle.net/21.15107/rcub_cer_5886"
}

Pavlović, R., Dojnov, B., Šokarda Slavić, M., Pavlović, M., Slomo, K., Ristović, M.,& Vujčić, Z.. (2021). Expression of amylases in adult honey bees fed with different patties. in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade, Serbia
Belgrade : University of Belgrade., 196.
https://hdl.handle.net/21.15107/rcub_cer_5886

Pavlović R, Dojnov B, Šokarda Slavić M, Pavlović M, Slomo K, Ristović M, Vujčić Z. Expression of amylases in adult honey bees fed with different patties. in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade, Serbia. 2021;:196.
https://hdl.handle.net/21.15107/rcub_cer_5886 .

Pavlović, Ratko, Dojnov, Biljana, Šokarda Slavić, Marinela, Pavlović, Marija, Slomo, Katarina, Ristović, Marina, Vujčić, Zoran, "Expression of amylases in adult honey bees fed with different patties" in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade, Serbia (2021):196,
https://hdl.handle.net/21.15107/rcub_cer_5886 .

TY  - CONF
AU  - Joksimović, Kristina
AU  - Slomo, Katarina
AU  - Avdalović, Jelena
AU  - Žerađanin, Aleksandra
AU  - Miletić, Srđan
AU  - Randjelović, Danijela
AU  - Beškoski, Vladimir
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5019
AB  - Description of a single chamber microbial fuel cells (MFC), its construction and operation, processing and analysis of experimental measurements in laboratory conditions is given
PB  - Leskovac: Faculty of Technology
C3  - 13 Symposium with international participation ''Novel Technologies and Economic Development'', Book of abstracts, Leskovac 18.-19. october, 2019.
T1  - Analytical aspects of a single-chamber system of microbial fuel cells (MFC)
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_cer_5019
ER  - 

@conference{
author = "Joksimović, Kristina and Slomo, Katarina and Avdalović, Jelena and Žerađanin, Aleksandra and Miletić, Srđan and Randjelović, Danijela and Beškoski, Vladimir",
year = "2019",
abstract = "Description of a single chamber microbial fuel cells (MFC), its construction and operation, processing and analysis of experimental measurements in laboratory conditions is given",
publisher = "Leskovac: Faculty of Technology",
journal = "13 Symposium with international participation ''Novel Technologies and Economic Development'', Book of abstracts, Leskovac 18.-19. october, 2019.",
title = "Analytical aspects of a single-chamber system of microbial fuel cells (MFC)",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_cer_5019"
}

Joksimović, K., Slomo, K., Avdalović, J., Žerađanin, A., Miletić, S., Randjelović, D.,& Beškoski, V.. (2019). Analytical aspects of a single-chamber system of microbial fuel cells (MFC). in 13 Symposium with international participation ''Novel Technologies and Economic Development'', Book of abstracts, Leskovac 18.-19. october, 2019.
Leskovac: Faculty of Technology., 57.
https://hdl.handle.net/21.15107/rcub_cer_5019

Joksimović K, Slomo K, Avdalović J, Žerađanin A, Miletić S, Randjelović D, Beškoski V. Analytical aspects of a single-chamber system of microbial fuel cells (MFC). in 13 Symposium with international participation ''Novel Technologies and Economic Development'', Book of abstracts, Leskovac 18.-19. october, 2019.. 2019;:57.
https://hdl.handle.net/21.15107/rcub_cer_5019 .

Joksimović, Kristina, Slomo, Katarina, Avdalović, Jelena, Žerađanin, Aleksandra, Miletić, Srđan, Randjelović, Danijela, Beškoski, Vladimir, "Analytical aspects of a single-chamber system of microbial fuel cells (MFC)" in 13 Symposium with international participation ''Novel Technologies and Economic Development'', Book of abstracts, Leskovac 18.-19. october, 2019. (2019):57,
https://hdl.handle.net/21.15107/rcub_cer_5019 .