TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Mišić, Milan
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5787
AB  - Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.
PB  - Elsevier Ltd
T2  - Fuel
T1  - Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch
VL  - 338
SP  - 127363
DO  - 10.1016/j.fuel.2022.127363
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Margetić, Aleksandra and Dojnov, Biljana and Vujčić, Miroslava and Mišić, Milan and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.",
publisher = "Elsevier Ltd",
journal = "Fuel",
title = "Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch",
volume = "338",
pages = "127363",
doi = "10.1016/j.fuel.2022.127363"
}

Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel
Elsevier Ltd., 338, 127363.
https://doi.org/10.1016/j.fuel.2022.127363

Šokarda Slavić M, Margetić A, Dojnov B, Vujčić M, Mišić M, Božić N, Vujčić Z. Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel. 2023;338:127363.
doi:10.1016/j.fuel.2022.127363 .

Šokarda Slavić, Marinela, Margetić, Aleksandra, Dojnov, Biljana, Vujčić, Miroslava, Mišić, Milan, Božić, Nataša, Vujčić, Zoran, "Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch" in Fuel, 338 (2023):127363,
https://doi.org/10.1016/j.fuel.2022.127363 . .

TY  - CONF
AU  - Pavlović, Ratko
AU  - Dojnov, Biljana
AU  - Šokarda Slavić, Marinela
AU  - Pavlović, Marija
AU  - Tomić, Nevena
AU  - Mišić, Milan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5885
AB  - Feeding of honey bee (Apis mellifera) is a challenge for beekeepers and formulation of food supplements is improved continuously. When natural food sources are scarce or not available, supplemental foods are widely used to support and build up honey bee colonies. Influence of commercial (sugar) patty and the patties enriched with 12.5% pollen and 12.5% dried yeast on protease expression in honey bee adults is presented. This is part of a wider study aimed to compare the expression of digestive enzymes using different patties. Data collected in this study can be useful for development of higher quality of food supplements for honey bees.
Honey bees were kept in an incubator for 21 days, at a temperature of 35°C and at 80% humidity. In each cage there were one hundred bees and a piece of honeycomb. Midgut samples were taken after 7, 14 and 21 days, homogenized and analyzed for protein concentration and protease activity.
The highest protein concentration was detected in the midgut of pollen fed bees, and lowest in commercial patty fed group, determined by the Bradford method. Protease activity was the highest in bee’s midgut fed with pollen patties, and the lowest in bees fed commercial patties which is shown by enzyme assay and by zymograms. There were different protease isoforms present in bees fed pollen, yeast and commercial patties, but the major isoforms were the same.
The observed decrease in protease activity over time in all groups is probably due to the transition to foraging tasks. Reduced protease activity in the gut of bees fed commercial patties is due to a lack of proteins or some other inducers present in food.
Because pollen is honey bee natural food, we conclude that food supplements that induce similar enzyme expression as pollen (dried yeast) can be considered as appropriate food substitution, because it is superior to supplements which induce very different enzyme expression.
PB  - University of Belgrade – Faculty of Biology
C3  - Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia
T1  - Expression of protease in adult honey bees fed with different patties
SP  - 172
UR  - https://hdl.handle.net/21.15107/rcub_cer_5885
ER  - 

@conference{
author = "Pavlović, Ratko and Dojnov, Biljana and Šokarda Slavić, Marinela and Pavlović, Marija and Tomić, Nevena and Mišić, Milan and Vujčić, Zoran",
year = "2022",
abstract = "Feeding of honey bee (Apis mellifera) is a challenge for beekeepers and formulation of food supplements is improved continuously. When natural food sources are scarce or not available, supplemental foods are widely used to support and build up honey bee colonies. Influence of commercial (sugar) patty and the patties enriched with 12.5% pollen and 12.5% dried yeast on protease expression in honey bee adults is presented. This is part of a wider study aimed to compare the expression of digestive enzymes using different patties. Data collected in this study can be useful for development of higher quality of food supplements for honey bees.
Honey bees were kept in an incubator for 21 days, at a temperature of 35°C and at 80% humidity. In each cage there were one hundred bees and a piece of honeycomb. Midgut samples were taken after 7, 14 and 21 days, homogenized and analyzed for protein concentration and protease activity.
The highest protein concentration was detected in the midgut of pollen fed bees, and lowest in commercial patty fed group, determined by the Bradford method. Protease activity was the highest in bee’s midgut fed with pollen patties, and the lowest in bees fed commercial patties which is shown by enzyme assay and by zymograms. There were different protease isoforms present in bees fed pollen, yeast and commercial patties, but the major isoforms were the same.
The observed decrease in protease activity over time in all groups is probably due to the transition to foraging tasks. Reduced protease activity in the gut of bees fed commercial patties is due to a lack of proteins or some other inducers present in food.
Because pollen is honey bee natural food, we conclude that food supplements that induce similar enzyme expression as pollen (dried yeast) can be considered as appropriate food substitution, because it is superior to supplements which induce very different enzyme expression.",
publisher = "University of Belgrade – Faculty of Biology",
journal = "Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia",
title = "Expression of protease in adult honey bees fed with different patties",
pages = "172",
url = "https://hdl.handle.net/21.15107/rcub_cer_5885"
}

Pavlović, R., Dojnov, B., Šokarda Slavić, M., Pavlović, M., Tomić, N., Mišić, M.,& Vujčić, Z.. (2022). Expression of protease in adult honey bees fed with different patties. in Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia
University of Belgrade – Faculty of Biology., 172.
https://hdl.handle.net/21.15107/rcub_cer_5885

Pavlović R, Dojnov B, Šokarda Slavić M, Pavlović M, Tomić N, Mišić M, Vujčić Z. Expression of protease in adult honey bees fed with different patties. in Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia. 2022;:172.
https://hdl.handle.net/21.15107/rcub_cer_5885 .

Pavlović, Ratko, Dojnov, Biljana, Šokarda Slavić, Marinela, Pavlović, Marija, Tomić, Nevena, Mišić, Milan, Vujčić, Zoran, "Expression of protease in adult honey bees fed with different patties" in Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia (2022):172,
https://hdl.handle.net/21.15107/rcub_cer_5885 .

TY  - CONF
AU  - Mišić, Milan
AU  - Drulović, Nenad
AU  - Margetić, Aleksandra
AU  - Vujčić, Zoran
AU  - Vujčić, Miroslava
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5841
AB  - Polyphenols are a large group of natural organic compounds mainly found in plants, in whom they have diverse protective and metabolic functions. It’s also known that phenolic compounds, especially tannins, interact with proteins in various significant and distinct ways. Tannins also complex proteins, which generally precipitates them and it’s their most important industrially utilised characteristic. This study, which was a continuation of our previous work, focused on interactions of aqueous tea plant extracts with laccase from Trametes 63ersicolour and β-amylase from Ipomoea batatas. Tea plants used in this study wereSaturejamontana, Menthapiperita, Salvia officinalis, Matricariachamomilla, Camellia cinensis and Arctostaphylosuva-ursi. Total phenolic content was determined using Folin-Ciocalteu reagent, which showed us that chosen plants vary considerably in their total phenolic content and the highest concentration was found to be in Arctostaphylosuva-ursi. Protein interactions between tea plant extracts were measured using spectrophotometric, spectrofluorimetric and electrophoretic methods. These methods showed that tea plant extracts lead to various structural changes within the protein, nature of which require 
further research. Finally, it was also found that all tea plant extracts, except Matricariachamomillaextract, reversibly precipitate β-amylase – whilst retaining most of 
its enzymatic activity after dissolving.Best results were obtained using Arctostaphylosuva-ursi extract, which precipitated the highest quantity of β-amylase, with highest activity retention. Although being an interesting phenomenon, furtherresearch is necessary to determine the nature andimportance of reversible tannic enzyme precipitation.
PB  - Serbian Chemical Society
C3  - Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
T1  - Protein interactions of six tea plant extracts
SP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_cer_5841
ER  - 

@conference{
author = "Mišić, Milan and Drulović, Nenad and Margetić, Aleksandra and Vujčić, Zoran and Vujčić, Miroslava",
year = "2022",
abstract = "Polyphenols are a large group of natural organic compounds mainly found in plants, in whom they have diverse protective and metabolic functions. It’s also known that phenolic compounds, especially tannins, interact with proteins in various significant and distinct ways. Tannins also complex proteins, which generally precipitates them and it’s their most important industrially utilised characteristic. This study, which was a continuation of our previous work, focused on interactions of aqueous tea plant extracts with laccase from Trametes 63ersicolour and β-amylase from Ipomoea batatas. Tea plants used in this study wereSaturejamontana, Menthapiperita, Salvia officinalis, Matricariachamomilla, Camellia cinensis and Arctostaphylosuva-ursi. Total phenolic content was determined using Folin-Ciocalteu reagent, which showed us that chosen plants vary considerably in their total phenolic content and the highest concentration was found to be in Arctostaphylosuva-ursi. Protein interactions between tea plant extracts were measured using spectrophotometric, spectrofluorimetric and electrophoretic methods. These methods showed that tea plant extracts lead to various structural changes within the protein, nature of which require 
further research. Finally, it was also found that all tea plant extracts, except Matricariachamomillaextract, reversibly precipitate β-amylase – whilst retaining most of 
its enzymatic activity after dissolving.Best results were obtained using Arctostaphylosuva-ursi extract, which precipitated the highest quantity of β-amylase, with highest activity retention. Although being an interesting phenomenon, furtherresearch is necessary to determine the nature andimportance of reversible tannic enzyme precipitation.",
publisher = "Serbian Chemical Society",
journal = "Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine",
title = "Protein interactions of six tea plant extracts",
pages = "63",
url = "https://hdl.handle.net/21.15107/rcub_cer_5841"
}

Mišić, M., Drulović, N., Margetić, A., Vujčić, Z.,& Vujčić, M.. (2022). Protein interactions of six tea plant extracts. in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
Serbian Chemical Society., 63.
https://hdl.handle.net/21.15107/rcub_cer_5841

Mišić M, Drulović N, Margetić A, Vujčić Z, Vujčić M. Protein interactions of six tea plant extracts. in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine. 2022;:63.
https://hdl.handle.net/21.15107/rcub_cer_5841 .

Mišić, Milan, Drulović, Nenad, Margetić, Aleksandra, Vujčić, Zoran, Vujčić, Miroslava, "Protein interactions of six tea plant extracts" in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine (2022):63,
https://hdl.handle.net/21.15107/rcub_cer_5841 .

TY  - CONF
AU  - Tomić, Nevena
AU  - Šokarda Slavić, Marinela
AU  - Ristović, Marina
AU  - Mišić, Milan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6457
AB  - Barley β-glucans are non-starchy polysaccharides that are composed of linear chains of β-D-(1→3) and β-D-(1→4) linked glucose residues. They comprise three (cellotriosyl) or four (cellotetraosyl) consecutive β-D-(1→4) bonded glucose units mutually connected via β-D-(1→3) glycosidic bonds. These polysaccharides can be found in the aleurone layer of the barley grain endosperm 1. It has been shown that β-glucans reduce blood cholesterol and influence postprandial glucose levels which makes them health beneficial. Also, they have been used as stabilizing and thickening agents in the food indutry1,2. Due to the presence of β-D-(1→3) glycosidic bond, β-glucans are water soluble. The extraction procedure of these polysaccharides involves several steps that include the inactivation of endogenous enzymes found in the grain, extraction with water or alkali solutions, implementation of hydrolytic enzymes so that starch and protein removal is possible and precipitation of β-glucans from the purified solution with alcohol3. We have developed a new protocol that enables the extraction of β-glucans without the coextraction of starch and the implementation of hydrolytic enzymes for starch removal, which makes the process of purification shorter and less expensive. The extracted and purified glucans were further characterized by FTIR spectroscopy and microanalysis
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia
T1  - Barley β-glucans: a new method for extraction and purification
SP  - 148
EP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_cer_6457
ER  - 

@conference{
author = "Tomić, Nevena and Šokarda Slavić, Marinela and Ristović, Marina and Mišić, Milan and Vujčić, Zoran",
year = "2022",
abstract = "Barley β-glucans are non-starchy polysaccharides that are composed of linear chains of β-D-(1→3) and β-D-(1→4) linked glucose residues. They comprise three (cellotriosyl) or four (cellotetraosyl) consecutive β-D-(1→4) bonded glucose units mutually connected via β-D-(1→3) glycosidic bonds. These polysaccharides can be found in the aleurone layer of the barley grain endosperm 1. It has been shown that β-glucans reduce blood cholesterol and influence postprandial glucose levels which makes them health beneficial. Also, they have been used as stabilizing and thickening agents in the food indutry1,2. Due to the presence of β-D-(1→3) glycosidic bond, β-glucans are water soluble. The extraction procedure of these polysaccharides involves several steps that include the inactivation of endogenous enzymes found in the grain, extraction with water or alkali solutions, implementation of hydrolytic enzymes so that starch and protein removal is possible and precipitation of β-glucans from the purified solution with alcohol3. We have developed a new protocol that enables the extraction of β-glucans without the coextraction of starch and the implementation of hydrolytic enzymes for starch removal, which makes the process of purification shorter and less expensive. The extracted and purified glucans were further characterized by FTIR spectroscopy and microanalysis",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia",
title = "Barley β-glucans: a new method for extraction and purification",
pages = "148-148",
url = "https://hdl.handle.net/21.15107/rcub_cer_6457"
}

Tomić, N., Šokarda Slavić, M., Ristović, M., Mišić, M.,& Vujčić, Z.. (2022). Barley β-glucans: a new method for extraction and purification. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia
University of Belgrade - Faculty of Chemistry., 148-148.
https://hdl.handle.net/21.15107/rcub_cer_6457

Tomić N, Šokarda Slavić M, Ristović M, Mišić M, Vujčić Z. Barley β-glucans: a new method for extraction and purification. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia. 2022;:148-148.
https://hdl.handle.net/21.15107/rcub_cer_6457 .

Tomić, Nevena, Šokarda Slavić, Marinela, Ristović, Marina, Mišić, Milan, Vujčić, Zoran, "Barley β-glucans: a new method for extraction and purification" in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia (2022):148-148,
https://hdl.handle.net/21.15107/rcub_cer_6457 .

TY  - CONF
AU  - Mišić, Milan
AU  - Margetić, Aleksandra
AU  - Vujčić, Zoran
AU  - Vujčić, Miroslava
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5839
AB  - Polyphenols are a large group of natural organic compounds mainly found in plants with diverse protective and metabolic functions. Phenolic compounds are well known for their antioxidant properties and the name “antioxidant” is mostly associated with them1. It’s also known that phenolic compounds, especially tannins, interact with proteins in various significant and distinct ways. This study was focused on examining these characteristics on aqueous extracts of tea plants local to Serbia (freshly picked Satureja montana, Mentha spicata, Salvia officinalis and Matricaria chamomilla), which are known to have various amounts and types of phenolic compounds. We examined total concentration of phenolic compounds, tannins, flavonoids, antioxidant activity and interactions with bovine serum albumin and whey protein. It was concluded that S. montana extract had the highest concentration of polyphenols and tannins. Flavonoid concentration was measured using the aluminium-chloride method and it was concluded that S. officinalis had the highest flavonoid content. Antioxidant activities were measured using DPPH, FRAP and ABTS, which are Single Electron Transfer (SET) mechanism-based antioxidant tests and ORAC which is a Hydrogen Atom Transfer mechanism-based antioxidant test. It was concluded that SET mechanism-based antioxidant activities correspond to the total concentration of polyphenols and tannins, which meant that S. montana extract had the highest antioxidant activity. S. officinalis extract exhibited the highest antioxidant activity measured by the ORAC assay, which corresponded to the highest Flavonoid concentration. Interactions between plant extracts and bovine serum albumin were measured via spectrophotometric and spectrofluorimetric titrations. It was concluded that S. montana exhibited most pronounced interactions with the protein. Nature of such interactions is still unknown but using SDS-PAGE it was concluded that proteins exhibit significant structural changes after interacting with plant extracts.
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
T1  - Antioxidant potential and protein interactions of four tea plant extracts
SP  - 107
EP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_cer_5839
ER  - 

@conference{
author = "Mišić, Milan and Margetić, Aleksandra and Vujčić, Zoran and Vujčić, Miroslava",
year = "2021",
abstract = "Polyphenols are a large group of natural organic compounds mainly found in plants with diverse protective and metabolic functions. Phenolic compounds are well known for their antioxidant properties and the name “antioxidant” is mostly associated with them1. It’s also known that phenolic compounds, especially tannins, interact with proteins in various significant and distinct ways. This study was focused on examining these characteristics on aqueous extracts of tea plants local to Serbia (freshly picked Satureja montana, Mentha spicata, Salvia officinalis and Matricaria chamomilla), which are known to have various amounts and types of phenolic compounds. We examined total concentration of phenolic compounds, tannins, flavonoids, antioxidant activity and interactions with bovine serum albumin and whey protein. It was concluded that S. montana extract had the highest concentration of polyphenols and tannins. Flavonoid concentration was measured using the aluminium-chloride method and it was concluded that S. officinalis had the highest flavonoid content. Antioxidant activities were measured using DPPH, FRAP and ABTS, which are Single Electron Transfer (SET) mechanism-based antioxidant tests and ORAC which is a Hydrogen Atom Transfer mechanism-based antioxidant test. It was concluded that SET mechanism-based antioxidant activities correspond to the total concentration of polyphenols and tannins, which meant that S. montana extract had the highest antioxidant activity. S. officinalis extract exhibited the highest antioxidant activity measured by the ORAC assay, which corresponded to the highest Flavonoid concentration. Interactions between plant extracts and bovine serum albumin were measured via spectrophotometric and spectrofluorimetric titrations. It was concluded that S. montana exhibited most pronounced interactions with the protein. Nature of such interactions is still unknown but using SDS-PAGE it was concluded that proteins exhibit significant structural changes after interacting with plant extracts.",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia",
title = "Antioxidant potential and protein interactions of four tea plant extracts",
pages = "107-107",
url = "https://hdl.handle.net/21.15107/rcub_cer_5839"
}

Mišić, M., Margetić, A., Vujčić, Z.,& Vujčić, M.. (2021). Antioxidant potential and protein interactions of four tea plant extracts. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
University of Belgrade - Faculty of Chemistry., 107-107.
https://hdl.handle.net/21.15107/rcub_cer_5839

Mišić M, Margetić A, Vujčić Z, Vujčić M. Antioxidant potential and protein interactions of four tea plant extracts. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia. 2021;:107-107.
https://hdl.handle.net/21.15107/rcub_cer_5839 .

Mišić, Milan, Margetić, Aleksandra, Vujčić, Zoran, Vujčić, Miroslava, "Antioxidant potential and protein interactions of four tea plant extracts" in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia (2021):107-107,
https://hdl.handle.net/21.15107/rcub_cer_5839 .