TY  - CONF
AU  - Pavlović, Ratko
AU  - Dojnov, Biljana
AU  - Šokarda Slavić, Marinela
AU  - Pavlović, Marija
AU  - Tomić, Nevena
AU  - Mišić, Milan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5885
AB  - Feeding of honey bee (Apis mellifera) is a challenge for beekeepers and formulation of food supplements is improved continuously. When natural food sources are scarce or not available, supplemental foods are widely used to support and build up honey bee colonies. Influence of commercial (sugar) patty and the patties enriched with 12.5% pollen and 12.5% dried yeast on protease expression in honey bee adults is presented. This is part of a wider study aimed to compare the expression of digestive enzymes using different patties. Data collected in this study can be useful for development of higher quality of food supplements for honey bees.
Honey bees were kept in an incubator for 21 days, at a temperature of 35°C and at 80% humidity. In each cage there were one hundred bees and a piece of honeycomb. Midgut samples were taken after 7, 14 and 21 days, homogenized and analyzed for protein concentration and protease activity.
The highest protein concentration was detected in the midgut of pollen fed bees, and lowest in commercial patty fed group, determined by the Bradford method. Protease activity was the highest in bee’s midgut fed with pollen patties, and the lowest in bees fed commercial patties which is shown by enzyme assay and by zymograms. There were different protease isoforms present in bees fed pollen, yeast and commercial patties, but the major isoforms were the same.
The observed decrease in protease activity over time in all groups is probably due to the transition to foraging tasks. Reduced protease activity in the gut of bees fed commercial patties is due to a lack of proteins or some other inducers present in food.
Because pollen is honey bee natural food, we conclude that food supplements that induce similar enzyme expression as pollen (dried yeast) can be considered as appropriate food substitution, because it is superior to supplements which induce very different enzyme expression.
PB  - University of Belgrade – Faculty of Biology
C3  - Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia
T1  - Expression of protease in adult honey bees fed with different patties
SP  - 172
UR  - https://hdl.handle.net/21.15107/rcub_cer_5885
ER  - 

@conference{
author = "Pavlović, Ratko and Dojnov, Biljana and Šokarda Slavić, Marinela and Pavlović, Marija and Tomić, Nevena and Mišić, Milan and Vujčić, Zoran",
year = "2022",
abstract = "Feeding of honey bee (Apis mellifera) is a challenge for beekeepers and formulation of food supplements is improved continuously. When natural food sources are scarce or not available, supplemental foods are widely used to support and build up honey bee colonies. Influence of commercial (sugar) patty and the patties enriched with 12.5% pollen and 12.5% dried yeast on protease expression in honey bee adults is presented. This is part of a wider study aimed to compare the expression of digestive enzymes using different patties. Data collected in this study can be useful for development of higher quality of food supplements for honey bees.
Honey bees were kept in an incubator for 21 days, at a temperature of 35°C and at 80% humidity. In each cage there were one hundred bees and a piece of honeycomb. Midgut samples were taken after 7, 14 and 21 days, homogenized and analyzed for protein concentration and protease activity.
The highest protein concentration was detected in the midgut of pollen fed bees, and lowest in commercial patty fed group, determined by the Bradford method. Protease activity was the highest in bee’s midgut fed with pollen patties, and the lowest in bees fed commercial patties which is shown by enzyme assay and by zymograms. There were different protease isoforms present in bees fed pollen, yeast and commercial patties, but the major isoforms were the same.
The observed decrease in protease activity over time in all groups is probably due to the transition to foraging tasks. Reduced protease activity in the gut of bees fed commercial patties is due to a lack of proteins or some other inducers present in food.
Because pollen is honey bee natural food, we conclude that food supplements that induce similar enzyme expression as pollen (dried yeast) can be considered as appropriate food substitution, because it is superior to supplements which induce very different enzyme expression.",
publisher = "University of Belgrade – Faculty of Biology",
journal = "Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia",
title = "Expression of protease in adult honey bees fed with different patties",
pages = "172",
url = "https://hdl.handle.net/21.15107/rcub_cer_5885"
}

Pavlović, R., Dojnov, B., Šokarda Slavić, M., Pavlović, M., Tomić, N., Mišić, M.,& Vujčić, Z.. (2022). Expression of protease in adult honey bees fed with different patties. in Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia
University of Belgrade – Faculty of Biology., 172.
https://hdl.handle.net/21.15107/rcub_cer_5885

Pavlović R, Dojnov B, Šokarda Slavić M, Pavlović M, Tomić N, Mišić M, Vujčić Z. Expression of protease in adult honey bees fed with different patties. in Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia. 2022;:172.
https://hdl.handle.net/21.15107/rcub_cer_5885 .

Pavlović, Ratko, Dojnov, Biljana, Šokarda Slavić, Marinela, Pavlović, Marija, Tomić, Nevena, Mišić, Milan, Vujčić, Zoran, "Expression of protease in adult honey bees fed with different patties" in Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia (2022):172,
https://hdl.handle.net/21.15107/rcub_cer_5885 .

TY  - CONF
AU  - Tomić, Nevena
AU  - Šokarda Slavić, Marinela
AU  - Ristović, Marina
AU  - Mišić, Milan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6457
AB  - Barley β-glucans are non-starchy polysaccharides that are composed of linear chains of β-D-(1→3) and β-D-(1→4) linked glucose residues. They comprise three (cellotriosyl) or four (cellotetraosyl) consecutive β-D-(1→4) bonded glucose units mutually connected via β-D-(1→3) glycosidic bonds. These polysaccharides can be found in the aleurone layer of the barley grain endosperm 1. It has been shown that β-glucans reduce blood cholesterol and influence postprandial glucose levels which makes them health beneficial. Also, they have been used as stabilizing and thickening agents in the food indutry1,2. Due to the presence of β-D-(1→3) glycosidic bond, β-glucans are water soluble. The extraction procedure of these polysaccharides involves several steps that include the inactivation of endogenous enzymes found in the grain, extraction with water or alkali solutions, implementation of hydrolytic enzymes so that starch and protein removal is possible and precipitation of β-glucans from the purified solution with alcohol3. We have developed a new protocol that enables the extraction of β-glucans without the coextraction of starch and the implementation of hydrolytic enzymes for starch removal, which makes the process of purification shorter and less expensive. The extracted and purified glucans were further characterized by FTIR spectroscopy and microanalysis
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia
T1  - Barley β-glucans: a new method for extraction and purification
SP  - 148
EP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_cer_6457
ER  - 

@conference{
author = "Tomić, Nevena and Šokarda Slavić, Marinela and Ristović, Marina and Mišić, Milan and Vujčić, Zoran",
year = "2022",
abstract = "Barley β-glucans are non-starchy polysaccharides that are composed of linear chains of β-D-(1→3) and β-D-(1→4) linked glucose residues. They comprise three (cellotriosyl) or four (cellotetraosyl) consecutive β-D-(1→4) bonded glucose units mutually connected via β-D-(1→3) glycosidic bonds. These polysaccharides can be found in the aleurone layer of the barley grain endosperm 1. It has been shown that β-glucans reduce blood cholesterol and influence postprandial glucose levels which makes them health beneficial. Also, they have been used as stabilizing and thickening agents in the food indutry1,2. Due to the presence of β-D-(1→3) glycosidic bond, β-glucans are water soluble. The extraction procedure of these polysaccharides involves several steps that include the inactivation of endogenous enzymes found in the grain, extraction with water or alkali solutions, implementation of hydrolytic enzymes so that starch and protein removal is possible and precipitation of β-glucans from the purified solution with alcohol3. We have developed a new protocol that enables the extraction of β-glucans without the coextraction of starch and the implementation of hydrolytic enzymes for starch removal, which makes the process of purification shorter and less expensive. The extracted and purified glucans were further characterized by FTIR spectroscopy and microanalysis",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia",
title = "Barley β-glucans: a new method for extraction and purification",
pages = "148-148",
url = "https://hdl.handle.net/21.15107/rcub_cer_6457"
}

Tomić, N., Šokarda Slavić, M., Ristović, M., Mišić, M.,& Vujčić, Z.. (2022). Barley β-glucans: a new method for extraction and purification. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia
University of Belgrade - Faculty of Chemistry., 148-148.
https://hdl.handle.net/21.15107/rcub_cer_6457

Tomić N, Šokarda Slavić M, Ristović M, Mišić M, Vujčić Z. Barley β-glucans: a new method for extraction and purification. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia. 2022;:148-148.
https://hdl.handle.net/21.15107/rcub_cer_6457 .

Tomić, Nevena, Šokarda Slavić, Marinela, Ristović, Marina, Mišić, Milan, Vujčić, Zoran, "Barley β-glucans: a new method for extraction and purification" in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia (2022):148-148,
https://hdl.handle.net/21.15107/rcub_cer_6457 .

TY  - CONF
AU  - Nenadović, Marija
AU  - Tomić, Nevena
AU  - Nikolić, Stefan
AU  - Vujčić, Zoran
AU  - Šokarda Slavić, Marinela
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6456
AB  - Zein is produced in large quantities as a byproduct of corn starch manufacturing since it
constitutes a majority of the total protein of maize seed (44–70%). Enzymatic treatment of
zein significantly improves its aqueous solubility and provides peptides that are used as
animal feed, functional food, or biologically active carriers for other bioactive molecules.
Moreover, zein-derived peptides exhibit antioxidant, anti-inflammatory, antihypertensive,
anticancer, and antimicrobial activities in human organisms 1. Few attempts up to this day
have been made to screen for microorganisms that are capable of zein degradation.
Available protocols for proteases identification almost exclusively rely on screening on
casein, skim milk, and gelatin agar in limited experimental conditions. We have screened
different Bacillus sp strains isolated from across Serbia for zein-degrading proteases. To
do so we developed an inexpensive, simple, and reproducible way of high throughput
functional screening of zein-degrading proteases on zein-containing gels. Besides detecting
proteases with specificity towards zein, a developed diffusion assay was designed to
support screening for naturally occurring robust proteases with high potential for industrial
application. By using classical methods of protein purification, we isolated an alkaline
thermostable protease from Bacillus amyloliquefaciens strain 12B that is resistant to the
presence of detergents, organic solvents, and high salt concentrations.
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
T1  - A search for nature’s robust proteases with zein as a substrate
SP  - 158
EP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_cer_6456
ER  - 

@conference{
author = "Nenadović, Marija and Tomić, Nevena and Nikolić, Stefan and Vujčić, Zoran and Šokarda Slavić, Marinela",
year = "2021",
abstract = "Zein is produced in large quantities as a byproduct of corn starch manufacturing since it
constitutes a majority of the total protein of maize seed (44–70%). Enzymatic treatment of
zein significantly improves its aqueous solubility and provides peptides that are used as
animal feed, functional food, or biologically active carriers for other bioactive molecules.
Moreover, zein-derived peptides exhibit antioxidant, anti-inflammatory, antihypertensive,
anticancer, and antimicrobial activities in human organisms 1. Few attempts up to this day
have been made to screen for microorganisms that are capable of zein degradation.
Available protocols for proteases identification almost exclusively rely on screening on
casein, skim milk, and gelatin agar in limited experimental conditions. We have screened
different Bacillus sp strains isolated from across Serbia for zein-degrading proteases. To
do so we developed an inexpensive, simple, and reproducible way of high throughput
functional screening of zein-degrading proteases on zein-containing gels. Besides detecting
proteases with specificity towards zein, a developed diffusion assay was designed to
support screening for naturally occurring robust proteases with high potential for industrial
application. By using classical methods of protein purification, we isolated an alkaline
thermostable protease from Bacillus amyloliquefaciens strain 12B that is resistant to the
presence of detergents, organic solvents, and high salt concentrations.",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia",
title = "A search for nature’s robust proteases with zein as a substrate",
pages = "158-158",
url = "https://hdl.handle.net/21.15107/rcub_cer_6456"
}

Nenadović, M., Tomić, N., Nikolić, S., Vujčić, Z.,& Šokarda Slavić, M.. (2021). A search for nature’s robust proteases with zein as a substrate. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
University of Belgrade - Faculty of Chemistry., 158-158.
https://hdl.handle.net/21.15107/rcub_cer_6456

Nenadović M, Tomić N, Nikolić S, Vujčić Z, Šokarda Slavić M. A search for nature’s robust proteases with zein as a substrate. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia. 2021;:158-158.
https://hdl.handle.net/21.15107/rcub_cer_6456 .

Nenadović, Marija, Tomić, Nevena, Nikolić, Stefan, Vujčić, Zoran, Šokarda Slavić, Marinela, "A search for nature’s robust proteases with zein as a substrate" in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia (2021):158-158,
https://hdl.handle.net/21.15107/rcub_cer_6456 .