TY  - JOUR
AU  - Đukanović, Stefana
AU  - Ganić, Tea
AU  - Lončarević, Branka
AU  - Cvetković, Stefana
AU  - Nikolić, Biljana
AU  - Tenji, Dina
AU  - Randjelović, Danijela
AU  - Mitić-Ćulafić, Dragana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5590
AB  - Aims Because the Staphylococcus aureus is one of the most well‐known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it.  Methods and Results Antibacterial activity was examined through microdilution assay. Antibiofilm testing included crystal violet staining of biofilm biomass and morphology analysis by Atomic force microscopy (AFM). Furthermore, aerobic respiration was monitored using the Micro‐Oxymax respirometer. For investigation of gene expression qRT‐PCR was performed. Emodin demonstrated strong antibacterial activity and ability to inhibit biofilm formation of all tested strains. The effect on preformed biofilms was spotted in few strains. AFM revealed that emodin affects biofilm structure and roughness. Monitoring of respiration under emodin treatment in planktonic and biofilm form revealed that emodin influenced aerobic respiration. Moreover, qRT‐PCR showed that emodin modulates expression of icaA, icaD, srrA and srrB genes, as well as RNAIII, and that this activity was strain‐specific.  Conclusion The results obtained in this study indicate the novel antibiofilm activity of emodin and its multiple pathways of action.  Significance and Impact of Study This is the first study that examined pathways through which emodin expressed its antibiofilm activity
PB  - Wiley
T2  - Journal of Applied Microbiology
T1  - Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms
VL  - 132
IS  - 3
SP  - 1840
EP  - 1855
DO  - 10.1111/jam.15360
ER  - 

@article{
author = "Đukanović, Stefana and Ganić, Tea and Lončarević, Branka and Cvetković, Stefana and Nikolić, Biljana and Tenji, Dina and Randjelović, Danijela and Mitić-Ćulafić, Dragana",
year = "2022",
abstract = "Aims Because the Staphylococcus aureus is one of the most well‐known pathogens associated with medical devices and nosocomial infections, the aim of the study was to examine antibiofilm potential of emodin against it.  Methods and Results Antibacterial activity was examined through microdilution assay. Antibiofilm testing included crystal violet staining of biofilm biomass and morphology analysis by Atomic force microscopy (AFM). Furthermore, aerobic respiration was monitored using the Micro‐Oxymax respirometer. For investigation of gene expression qRT‐PCR was performed. Emodin demonstrated strong antibacterial activity and ability to inhibit biofilm formation of all tested strains. The effect on preformed biofilms was spotted in few strains. AFM revealed that emodin affects biofilm structure and roughness. Monitoring of respiration under emodin treatment in planktonic and biofilm form revealed that emodin influenced aerobic respiration. Moreover, qRT‐PCR showed that emodin modulates expression of icaA, icaD, srrA and srrB genes, as well as RNAIII, and that this activity was strain‐specific.  Conclusion The results obtained in this study indicate the novel antibiofilm activity of emodin and its multiple pathways of action.  Significance and Impact of Study This is the first study that examined pathways through which emodin expressed its antibiofilm activity",
publisher = "Wiley",
journal = "Journal of Applied Microbiology",
title = "Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms",
volume = "132",
number = "3",
pages = "1840-1855",
doi = "10.1111/jam.15360"
}

Đukanović, S., Ganić, T., Lončarević, B., Cvetković, S., Nikolić, B., Tenji, D., Randjelović, D.,& Mitić-Ćulafić, D.. (2022). Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms. in Journal of Applied Microbiology
Wiley., 132(3), 1840-1855.
https://doi.org/10.1111/jam.15360

Đukanović S, Ganić T, Lončarević B, Cvetković S, Nikolić B, Tenji D, Randjelović D, Mitić-Ćulafić D. Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms. in Journal of Applied Microbiology. 2022;132(3):1840-1855.
doi:10.1111/jam.15360 .

Đukanović, Stefana, Ganić, Tea, Lončarević, Branka, Cvetković, Stefana, Nikolić, Biljana, Tenji, Dina, Randjelović, Danijela, Mitić-Ćulafić, Dragana, "Elucidating the antibiofilm activity of Frangula emodin against Staphylococcus aureus biofilms" in Journal of Applied Microbiology, 132, no. 3 (2022):1840-1855,
https://doi.org/10.1111/jam.15360 . .

TY  - CONF
AU  - Đukanović, Stefana
AU  - Lješević, Marija
AU  - Simin, Nataša
AU  - Lončarević, Branka
AU  - Ganić, Tea
AU  - Nikolić, Biljana
AU  - Cvetković, Stefana
AU  - Mitić-Ćulafić, Dragana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5148
AB  - The aim of the study was to chemically characterize the ethyl-acetate extract of F. alnus bark and investigate its antioxidant and antibacterial potential. Qualitative analysis of extract was done by GCxGC-MS while selected phenolics were quantified by LC-MS/MS analysis.
PB  - Opole, Poland : University of Opole
C3  - QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland
T1  - Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract
SP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_cer_5148
ER  - 

@conference{
author = "Đukanović, Stefana and Lješević, Marija and Simin, Nataša and Lončarević, Branka and Ganić, Tea and Nikolić, Biljana and Cvetković, Stefana and Mitić-Ćulafić, Dragana",
year = "2021",
abstract = "The aim of the study was to chemically characterize the ethyl-acetate extract of F. alnus bark and investigate its antioxidant and antibacterial potential. Qualitative analysis of extract was done by GCxGC-MS while selected phenolics were quantified by LC-MS/MS analysis.",
publisher = "Opole, Poland : University of Opole",
journal = "QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland",
title = "Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract",
pages = "147",
url = "https://hdl.handle.net/21.15107/rcub_cer_5148"
}

Đukanović, S., Lješević, M., Simin, N., Lončarević, B., Ganić, T., Nikolić, B., Cvetković, S.,& Mitić-Ćulafić, D.. (2021). Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract. in QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland
Opole, Poland : University of Opole., 147.
https://hdl.handle.net/21.15107/rcub_cer_5148

Đukanović S, Lješević M, Simin N, Lončarević B, Ganić T, Nikolić B, Cvetković S, Mitić-Ćulafić D. Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract. in QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland. 2021;:147.
https://hdl.handle.net/21.15107/rcub_cer_5148 .

Đukanović, Stefana, Lješević, Marija, Simin, Nataša, Lončarević, Branka, Ganić, Tea, Nikolić, Biljana, Cvetković, Stefana, Mitić-Ćulafić, Dragana, "Chemical caracterization and investigation of biological activities of frangula alnus ethylacetate extract" in QUO VADIS Life Sciences Conference, Abstract book, 23-27 June 2021, Opole, Poland (2021):147,
https://hdl.handle.net/21.15107/rcub_cer_5148 .

TY  - JOUR
AU  - Jovanović, Marina
AU  - Zlatanović, Snežana
AU  - Micić, Darko
AU  - Bacić, Dragan
AU  - Mitić-Ćulafić, Dragana
AU  - Đuriš, Mihal
AU  - Gorjanović, Stanislava Ž.
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5957
AB  - Following the idea of sustainability in food production, a yogurt premix based on beetroot (Beta vulgaris) pomace flour (BPF) was developed. BPF was granulated with lactose solution
containing lactic acid bacteria (LAB) by a fluidized bed. Particle size increased ~30%. A decrease in
Carr Index from 21.5 to 14.98 and Hausner ratio from 1.27 to 1.18 confirmed improved flowability
of granulated BPF, whereas a decrease in water activity implied better storability. Yogurts were
produced weekly from neat starters and granulated BPF (3% w/w) that were stored for up to one
month (4 ◦C). High viability of Streptococcus thermophilus was observed. Less pronounced syneresis,
higher inhibition of colon cancer cell viability (13.0–24.5%), and anti-Escherichia activity were ascribed
to BPF yogurts or their supernatants (i.e., extracted whey). Acceptable palatability for humans and
dogs was demonstrated. A survey revealed positive consumers’ attitudes toward the granulated
BPF as a premix for yogurts amended to humans and dogs. For the first time, BPF granulated with
LAB was used as a premix for a fermented beverage. An initial step in the conceptualization of a
novel DIY (do it yourself) formula for obtaining a fresh yogurt fortified with natural dietary fiber
and antioxidants has been accomplished.
PB  - MDPI
T2  - Foods
T1  - Functionality and Palatability of Yogurt Produced Using Beetroot Pomace Flour Granulated with Lactic Acid Bacteria
VL  - 10
IS  - 8
SP  - 1696
DO  - 10.3390/foods10081696
ER  - 

@article{
author = "Jovanović, Marina and Zlatanović, Snežana and Micić, Darko and Bacić, Dragan and Mitić-Ćulafić, Dragana and Đuriš, Mihal and Gorjanović, Stanislava Ž.",
year = "2021",
abstract = "Following the idea of sustainability in food production, a yogurt premix based on beetroot (Beta vulgaris) pomace flour (BPF) was developed. BPF was granulated with lactose solution
containing lactic acid bacteria (LAB) by a fluidized bed. Particle size increased ~30%. A decrease in
Carr Index from 21.5 to 14.98 and Hausner ratio from 1.27 to 1.18 confirmed improved flowability
of granulated BPF, whereas a decrease in water activity implied better storability. Yogurts were
produced weekly from neat starters and granulated BPF (3% w/w) that were stored for up to one
month (4 ◦C). High viability of Streptococcus thermophilus was observed. Less pronounced syneresis,
higher inhibition of colon cancer cell viability (13.0–24.5%), and anti-Escherichia activity were ascribed
to BPF yogurts or their supernatants (i.e., extracted whey). Acceptable palatability for humans and
dogs was demonstrated. A survey revealed positive consumers’ attitudes toward the granulated
BPF as a premix for yogurts amended to humans and dogs. For the first time, BPF granulated with
LAB was used as a premix for a fermented beverage. An initial step in the conceptualization of a
novel DIY (do it yourself) formula for obtaining a fresh yogurt fortified with natural dietary fiber
and antioxidants has been accomplished.",
publisher = "MDPI",
journal = "Foods",
title = "Functionality and Palatability of Yogurt Produced Using Beetroot Pomace Flour Granulated with Lactic Acid Bacteria",
volume = "10",
number = "8",
pages = "1696",
doi = "10.3390/foods10081696"
}

Jovanović, M., Zlatanović, S., Micić, D., Bacić, D., Mitić-Ćulafić, D., Đuriš, M.,& Gorjanović, S. Ž.. (2021). Functionality and Palatability of Yogurt Produced Using Beetroot Pomace Flour Granulated with Lactic Acid Bacteria. in Foods
MDPI., 10(8), 1696.
https://doi.org/10.3390/foods10081696

Jovanović M, Zlatanović S, Micić D, Bacić D, Mitić-Ćulafić D, Đuriš M, Gorjanović SŽ. Functionality and Palatability of Yogurt Produced Using Beetroot Pomace Flour Granulated with Lactic Acid Bacteria. in Foods. 2021;10(8):1696.
doi:10.3390/foods10081696 .

Jovanović, Marina, Zlatanović, Snežana, Micić, Darko, Bacić, Dragan, Mitić-Ćulafić, Dragana, Đuriš, Mihal, Gorjanović, Stanislava Ž., "Functionality and Palatability of Yogurt Produced Using Beetroot Pomace Flour Granulated with Lactic Acid Bacteria" in Foods, 10, no. 8 (2021):1696,
https://doi.org/10.3390/foods10081696 . .

TY  - JOUR
AU  - Đukanović, Stefana
AU  - Cvetković, Stefana
AU  - Lončarević, Branka
AU  - Lješević, Marija
AU  - Nikolić, Biljana
AU  - Simin, Nataša
AU  - Bekvalac, Kristina
AU  - Kekić, Dušan
AU  - Mitić-Ćulafić, Dragana
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3946
AB  - Staphylococcus aureus is one of the leading causative of numerous infections that is resistant to various antibiotics. Since that we are facing with a serious problem of bacterial resistance it is necessary to find a new antibacterial agents in fight with it. Frangula alnus is traditionally used plant but its antibiofilm potential is poorly investigated. The aim of the study was to investigate antibiofilm activity of F. alnus ethyl-acetate extract (FA) toward S. aureus ATCC strains and clinical isolates as well as its influence on respiration in planktonic and biofilm form. The qualitative GCxGC–MS and quantitative LC–MS/MS analysis revealed that FA extract was rich in phenols and flavonoids and emodin, chatechin, and ester 4-ethoxy benzoic acid were the most dominant components. Results obtained through microdillution assay showed that FA possesses strong antibacterial activity. Furthermore, crystal violet staining of biofilm biomass demonstrated that extract had strong effect on biofilm formation of all tested strains while effect on preformed biofilms was less pronounced. The effect on biofilm was confirmed with scanning electron microscopy where the changes in biofilm structure were noticed. The activity of extract on the consumption of O2 and production of CO2 was monitored using the Micro-Oxymax respirometer. Interestingly, respiration of the most strains was decreased in planktonic form as well as in biofilms. Results obtained in this study are a good basis for further research in order to discover the mechanism of action of the FA extract on connection between biofilm and respiration.
PB  - Elsevier
T2  - Industrial Crops & Products
T1  - Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract
VL  - 158
SP  - 113013
DO  - 10.1016/j.indcrop.2020.113013
ER  - 

@article{
author = "Đukanović, Stefana and Cvetković, Stefana and Lončarević, Branka and Lješević, Marija and Nikolić, Biljana and Simin, Nataša and Bekvalac, Kristina and Kekić, Dušan and Mitić-Ćulafić, Dragana",
year = "2020",
abstract = "Staphylococcus aureus is one of the leading causative of numerous infections that is resistant to various antibiotics. Since that we are facing with a serious problem of bacterial resistance it is necessary to find a new antibacterial agents in fight with it. Frangula alnus is traditionally used plant but its antibiofilm potential is poorly investigated. The aim of the study was to investigate antibiofilm activity of F. alnus ethyl-acetate extract (FA) toward S. aureus ATCC strains and clinical isolates as well as its influence on respiration in planktonic and biofilm form. The qualitative GCxGC–MS and quantitative LC–MS/MS analysis revealed that FA extract was rich in phenols and flavonoids and emodin, chatechin, and ester 4-ethoxy benzoic acid were the most dominant components. Results obtained through microdillution assay showed that FA possesses strong antibacterial activity. Furthermore, crystal violet staining of biofilm biomass demonstrated that extract had strong effect on biofilm formation of all tested strains while effect on preformed biofilms was less pronounced. The effect on biofilm was confirmed with scanning electron microscopy where the changes in biofilm structure were noticed. The activity of extract on the consumption of O2 and production of CO2 was monitored using the Micro-Oxymax respirometer. Interestingly, respiration of the most strains was decreased in planktonic form as well as in biofilms. Results obtained in this study are a good basis for further research in order to discover the mechanism of action of the FA extract on connection between biofilm and respiration.",
publisher = "Elsevier",
journal = "Industrial Crops & Products",
title = "Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract",
volume = "158",
pages = "113013",
doi = "10.1016/j.indcrop.2020.113013"
}

Đukanović, S., Cvetković, S., Lončarević, B., Lješević, M., Nikolić, B., Simin, N., Bekvalac, K., Kekić, D.,& Mitić-Ćulafić, D.. (2020). Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract. in Industrial Crops & Products
Elsevier., 158, 113013.
https://doi.org/10.1016/j.indcrop.2020.113013

Đukanović S, Cvetković S, Lončarević B, Lješević M, Nikolić B, Simin N, Bekvalac K, Kekić D, Mitić-Ćulafić D. Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract. in Industrial Crops & Products. 2020;158:113013.
doi:10.1016/j.indcrop.2020.113013 .

Đukanović, Stefana, Cvetković, Stefana, Lončarević, Branka, Lješević, Marija, Nikolić, Biljana, Simin, Nataša, Bekvalac, Kristina, Kekić, Dušan, Mitić-Ćulafić, Dragana, "Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract" in Industrial Crops & Products, 158 (2020):113013,
https://doi.org/10.1016/j.indcrop.2020.113013 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Nikolić, Gorana
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Pejić, Snežana
AU  - Martinović, Vesna
AU  - Mitić-Ćulafić, Dragana
AU  - Milić, Dragana
AU  - Kop, Tatjana
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3488
AB  - The effects of twelve weeks of supplementation with fullerene C60 olive/coconut oil solution on a broad spectrum of parameters in rats were examined. The tissue bioaccumulation of C60 was shown to be tissue-specific, with the liver, heart, and adrenal glands being the organs of the greatest, and the kidney, brain, and spleen being the organs of the smallest accumulation. C60 did not change aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase serum activities level, nor the damage of liver cells DNA. There were no effects of fullerene on prooxidant-antioxidant balance in the liver, kidney, spleen, heart, and brain, nor any visible harmful effects on the liver, heart, aorta, spleen, kidney, and small intestine histology. Fullerene changed the gut microbiota structure towards the bacteria that ameliorate lipid homeostasis, causing a serum triglycerides concentration decrease. However, C60 significantly increased the insulin resistance, serum ascorbate oxidation, and brain malondialdehyde and advanced oxidation protein products level. The deteriorative effects of C60 on the brain and serum could be attributed to the specific physicochemical composition of these tissues, potentiating the C60 aggregation or biotransformation as the key element of its pro-oxidative action.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats
VL  - 140
SP  - 111322
DO  - 10.1016/j.fct.2020.111302
ER  - 

@article{
author = "Đurašević, Siniša and Nikolić, Gorana and Todorović, Ana and Drakulić, Dunja and Pejić, Snežana and Martinović, Vesna and Mitić-Ćulafić, Dragana and Milić, Dragana and Kop, Tatjana and Jasnić, Nebojša and Đorđević, Jelena and Todorović, Zoran",
year = "2020",
abstract = "The effects of twelve weeks of supplementation with fullerene C60 olive/coconut oil solution on a broad spectrum of parameters in rats were examined. The tissue bioaccumulation of C60 was shown to be tissue-specific, with the liver, heart, and adrenal glands being the organs of the greatest, and the kidney, brain, and spleen being the organs of the smallest accumulation. C60 did not change aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase serum activities level, nor the damage of liver cells DNA. There were no effects of fullerene on prooxidant-antioxidant balance in the liver, kidney, spleen, heart, and brain, nor any visible harmful effects on the liver, heart, aorta, spleen, kidney, and small intestine histology. Fullerene changed the gut microbiota structure towards the bacteria that ameliorate lipid homeostasis, causing a serum triglycerides concentration decrease. However, C60 significantly increased the insulin resistance, serum ascorbate oxidation, and brain malondialdehyde and advanced oxidation protein products level. The deteriorative effects of C60 on the brain and serum could be attributed to the specific physicochemical composition of these tissues, potentiating the C60 aggregation or biotransformation as the key element of its pro-oxidative action.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats",
volume = "140",
pages = "111322",
doi = "10.1016/j.fct.2020.111302"
}

Đurašević, S., Nikolić, G., Todorović, A., Drakulić, D., Pejić, S., Martinović, V., Mitić-Ćulafić, D., Milić, D., Kop, T., Jasnić, N., Đorđević, J.,& Todorović, Z.. (2020). Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats. in Food and Chemical Toxicology
Elsevier., 140, 111322.
https://doi.org/10.1016/j.fct.2020.111302

Đurašević S, Nikolić G, Todorović A, Drakulić D, Pejić S, Martinović V, Mitić-Ćulafić D, Milić D, Kop T, Jasnić N, Đorđević J, Todorović Z. Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats. in Food and Chemical Toxicology. 2020;140:111322.
doi:10.1016/j.fct.2020.111302 .

Đurašević, Siniša, Nikolić, Gorana, Todorović, Ana, Drakulić, Dunja, Pejić, Snežana, Martinović, Vesna, Mitić-Ćulafić, Dragana, Milić, Dragana, Kop, Tatjana, Jasnić, Nebojša, Đorđević, Jelena, Todorović, Zoran, "Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats" in Food and Chemical Toxicology, 140 (2020):111322,
https://doi.org/10.1016/j.fct.2020.111302 . .