TY  - JOUR
AU  - Tasić, Ljubica
AU  - Avramović, Nataša
AU  - Quintero, Melissa
AU  - Stanišić, Danijela
AU  - Martins, Lucas
AU  - Brena da Costa, Tássia
AU  - Jadranin, Milka
AU  - Accioly de Souza, Maria Theresa
AU  - Faria, Paulo
AU  - de Camargo, Beatriz
AU  - de Sá Pereira, Bruna Maria
AU  - Maschietto, Mariana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5323
AB  - Pediatric cancer NMR-metabonomics might be a powerful tool to discover modified
biochemical pathways in tumor development, improve cancer diagnosis, and, consequently, treatment.
Wilms tumor (WT) is the most common kidney tumor in young children whose genetic and epigenetic
abnormalities lead to cell metabolism alterations, but, so far, investigation of metabolic pathways
in WT is scarce. We aimed to explore the high-resolution magic-angle spinning nuclear magnetic
resonance (HR-MAS NMR) metabonomics of WT and normal kidney (NK) samples. For this study,
14 WT and 7 NK tissue samples were obtained from the same patients and analyzed. One-dimensional
and two-dimensional HR-MAS NMR spectra were processed, and the one-dimensional NMR data
were analyzed using chemometrics. Chemometrics enabled us to elucidate the most significant
differences between the tumor and normal tissues and to discover intrinsic metabolite alterations in
WT. The metabolic differences in WT tissues were revealed by a validated PLS-DA applied on HR MAS T2
-edited 1H-NMR and were assigned to 16 metabolites, such as lipids, glucose, and branched chain amino acids (BCAAs), among others. The WT compared to NK samples showed 13 metabolites
with increased concentrations and 3 metabolites with decreased concentrations. The relative BCAA
concentrations were decreased in the WT while lipids, lactate, and glutamine/glutamate showed
increased levels. Sixteen tissue metabolites distinguish the analyzed WT samples and point to altered
glycolysis, glutaminolysis, TCA cycle, and lipid and BCAA metabolism in WT. Significant variation
in the concentrations of metabolites, such as glutamine/glutamate, lipids, lactate, and BCAAs, was
observed in WT and opened up a perspective for their further study and clinical validation.
PB  - MDPI
T2  - Diagnostic
T1  - A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy
VL  - 12
IS  - 1
SP  - 2
EP  - 10
DO  - 10.3390/diagnostics12010157
ER  - 

@article{
author = "Tasić, Ljubica and Avramović, Nataša and Quintero, Melissa and Stanišić, Danijela and Martins, Lucas and Brena da Costa, Tássia and Jadranin, Milka and Accioly de Souza, Maria Theresa and Faria, Paulo and de Camargo, Beatriz and de Sá Pereira, Bruna Maria and Maschietto, Mariana",
year = "2022",
abstract = "Pediatric cancer NMR-metabonomics might be a powerful tool to discover modified
biochemical pathways in tumor development, improve cancer diagnosis, and, consequently, treatment.
Wilms tumor (WT) is the most common kidney tumor in young children whose genetic and epigenetic
abnormalities lead to cell metabolism alterations, but, so far, investigation of metabolic pathways
in WT is scarce. We aimed to explore the high-resolution magic-angle spinning nuclear magnetic
resonance (HR-MAS NMR) metabonomics of WT and normal kidney (NK) samples. For this study,
14 WT and 7 NK tissue samples were obtained from the same patients and analyzed. One-dimensional
and two-dimensional HR-MAS NMR spectra were processed, and the one-dimensional NMR data
were analyzed using chemometrics. Chemometrics enabled us to elucidate the most significant
differences between the tumor and normal tissues and to discover intrinsic metabolite alterations in
WT. The metabolic differences in WT tissues were revealed by a validated PLS-DA applied on HR MAS T2
-edited 1H-NMR and were assigned to 16 metabolites, such as lipids, glucose, and branched chain amino acids (BCAAs), among others. The WT compared to NK samples showed 13 metabolites
with increased concentrations and 3 metabolites with decreased concentrations. The relative BCAA
concentrations were decreased in the WT while lipids, lactate, and glutamine/glutamate showed
increased levels. Sixteen tissue metabolites distinguish the analyzed WT samples and point to altered
glycolysis, glutaminolysis, TCA cycle, and lipid and BCAA metabolism in WT. Significant variation
in the concentrations of metabolites, such as glutamine/glutamate, lipids, lactate, and BCAAs, was
observed in WT and opened up a perspective for their further study and clinical validation.",
publisher = "MDPI",
journal = "Diagnostic",
title = "A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy",
volume = "12",
number = "1",
pages = "2-10",
doi = "10.3390/diagnostics12010157"
}

Tasić, L., Avramović, N., Quintero, M., Stanišić, D., Martins, L., Brena da Costa, T., Jadranin, M., Accioly de Souza, M. T., Faria, P., de Camargo, B., de Sá Pereira, B. M.,& Maschietto, M.. (2022). A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy. in Diagnostic
MDPI., 12(1), 2-10.
https://doi.org/10.3390/diagnostics12010157

Tasić L, Avramović N, Quintero M, Stanišić D, Martins L, Brena da Costa T, Jadranin M, Accioly de Souza MT, Faria P, de Camargo B, de Sá Pereira BM, Maschietto M. A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy. in Diagnostic. 2022;12(1):2-10.
doi:10.3390/diagnostics12010157 .

Tasić, Ljubica, Avramović, Nataša, Quintero, Melissa, Stanišić, Danijela, Martins, Lucas, Brena da Costa, Tássia, Jadranin, Milka, Accioly de Souza, Maria Theresa, Faria, Paulo, de Camargo, Beatriz, de Sá Pereira, Bruna Maria, Maschietto, Mariana, "A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy" in Diagnostic, 12, no. 1 (2022):2-10,
https://doi.org/10.3390/diagnostics12010157 . .

TY  - CONF
AU  - Tasić, Ljubica
AU  - Martins, Lucas
AU  - Braga, Erik Sobrinho
AU  - Novais, M.
AU  - Jadranin, Milka
AU  - Stanišić, Danijela
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7277
AB  - The infection with SARS-CoV-2 mostly leads to mild disease, but some individuals progress to severe disease with death outcome [1]. These severe cases are driven by the host response to the infection, with multisystem dysfunctions [1,2]. Timely intervention in patients in critical conditions could be crucial for their survival. Therefore, it is of paramount importance to understand the disease phase and its progression markers. Serum samples from two cohorts of patients, with mild (n = 92) and severe disease (n = 41) were analyzed, with two subgroups in each cohort, positive and negative to the virus. The samples were age and gender-matched to healthy volunteers (n = 87), too. Three NMR data sets (1H-NMR, T2, and edited by diffusion) were recorded on a 600 MHz Bruker AVANCE III spectrometer, and analyzed in MetaboAnalyst 5.0. Serum metabolomics showed that proteins, very low and low-density lipoproteins (VLDL and LDL), phospholipids, 
lysophosphatidylcholines among other 50 metabolites were disrupted. Furthermore, changes in the levels of NAD(P)+, hyperglycemia and dysregulated glucose were detrimental in people with COVID-19. Glu cose excess, increased levels of VLDL, and decreased proteins were related to the disease severity, and might be important for determining a patient’s phase of the disease, and NMR can greatly add to the differentiation between severe and mild symptoms.
PB  - NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)
C3  - 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event
T1  - Impressions on COVID-19 by NMR
SP  - OC24
UR  - https://hdl.handle.net/21.15107/rcub_cer_7277
ER  - 

@conference{
author = "Tasić, Ljubica and Martins, Lucas and Braga, Erik Sobrinho and Novais, M. and Jadranin, Milka and Stanišić, Danijela",
year = "2021",
abstract = "The infection with SARS-CoV-2 mostly leads to mild disease, but some individuals progress to severe disease with death outcome [1]. These severe cases are driven by the host response to the infection, with multisystem dysfunctions [1,2]. Timely intervention in patients in critical conditions could be crucial for their survival. Therefore, it is of paramount importance to understand the disease phase and its progression markers. Serum samples from two cohorts of patients, with mild (n = 92) and severe disease (n = 41) were analyzed, with two subgroups in each cohort, positive and negative to the virus. The samples were age and gender-matched to healthy volunteers (n = 87), too. Three NMR data sets (1H-NMR, T2, and edited by diffusion) were recorded on a 600 MHz Bruker AVANCE III spectrometer, and analyzed in MetaboAnalyst 5.0. Serum metabolomics showed that proteins, very low and low-density lipoproteins (VLDL and LDL), phospholipids, 
lysophosphatidylcholines among other 50 metabolites were disrupted. Furthermore, changes in the levels of NAD(P)+, hyperglycemia and dysregulated glucose were detrimental in people with COVID-19. Glu cose excess, increased levels of VLDL, and decreased proteins were related to the disease severity, and might be important for determining a patient’s phase of the disease, and NMR can greatly add to the differentiation between severe and mild symptoms.",
publisher = "NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)",
journal = "10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event",
title = "Impressions on COVID-19 by NMR",
pages = "OC24",
url = "https://hdl.handle.net/21.15107/rcub_cer_7277"
}

Tasić, L., Martins, L., Braga, E. S., Novais, M., Jadranin, M.,& Stanišić, D.. (2021). Impressions on COVID-19 by NMR. in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event
NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)., OC24.
https://hdl.handle.net/21.15107/rcub_cer_7277

Tasić L, Martins L, Braga ES, Novais M, Jadranin M, Stanišić D. Impressions on COVID-19 by NMR. in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event. 2021;:OC24.
https://hdl.handle.net/21.15107/rcub_cer_7277 .

Tasić, Ljubica, Martins, Lucas, Braga, Erik Sobrinho, Novais, M., Jadranin, Milka, Stanišić, Danijela, "Impressions on COVID-19 by NMR" in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event (2021):OC24,
https://hdl.handle.net/21.15107/rcub_cer_7277 .