TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Marinković, Vesna
AU  - Djurović, Dijana
AU  - Mileusnić Milenović, Radmila
AU  - Avdalović, Jelena
AU  - Vrvić, Miroslav
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7235
AB  - Although human breast milk contains significant biomolecules
with major impact on growth and development of preterm babies, a diet based exclusively on this milk over a longer period of
time can be associated with the development of micronutrient
deficiency in this sensitive group of infants. The aim of this study
was to determine the content of micronutrients in human breast
milk from the mothers of preterm infants and in infant formula,
and the influence of mineral content on the osmolality and potential renal solute load. Human breast milk taken during the
lactation period was examined, before and after frozen storage,
pasteurization and supplementation with a fortifier, with mineral
content being determined by inductively coupled plasma-optical
emission spectrometry. Osmolality of preterm milk and infant formula was measured using an osmometer. During the lactation
period of mothers of preterm born babies, the mineral content
of the breast milk changed. The concentrations of all examined
minerals differed in colostrum and in mature milk. However, there
were no significant differences in mineral content in mature milk
before and after pasteurization and storage. Supplementation of
mature milk with a fortifier increased the concentration of minerals, the final osmolality and the potential renal solute load. The
mineral content of mature milk was lower than necessary for the
optimal growth of preterm infants, so adequate supplementation
with a fortifier is needed to provide biologically important minerals. However, the osmolarity of supplemented milk should be
monitored due to the potentially increased pressure on the kidneys of preterm infants
AB  - Iako humano mleko sadrži brojne biomolekule značajne za rast i razvoj prevremeno rođenih beba, ishrana bazirana isključivo na majčinom mleku tokom dužeg vremenskog perioda može biti povezana sa nedostatkom mikronutrijenata kod ove osetljive grupe odojčadi. Cilj ovog istraživanja je da se utvrdi sadržaj minerala u mleku majki prevremeno rođene dece i infant formuli, kao i njihov uticaj na osmolalitet, osmolaritet i potencijalno opterećenje bubrega rastvornim supstancama. Mineralne komponente infant formule i majčinog mleka u toku perioda laktacije, nakon skladištenja pasterizacije i suplementacije obogaćivačem, određene su primenom tehnike indukovano spregnute plazme - optičke emisione spektrometrije. Osmolalnost uzoraka mleka i infant formule je određena osmometrom. Tokom perioda laktacije majki prevremeno rođenih beba menja se sadržaj minerala u mleku. Koncentracije ispitivanih minerala su se razlikovale u zrelom mleku odnosu na kolostrum. Nije bilo značajne razlike u sadržaju minerala u zrelom mleku pre i nakon pasterizacije i zamrzavanja. Suplementacija zrelog mleka obogaćivačem povećava koncentraciju minerala, ukupnu osmolalnost i potencijalno opterećenje bubrega. Mineralni sadržaj zrelog mleka je manji od potrebnog za optimalan rast prevremeno rođene dece, stoga je neophodna adekvatna suplementacija obogaćivačem kojim se obezbeđuju biološki važni minerali. Međutim, potrebno je pratiti osmolarnost suplementiranog mleka zbog potencijalno povećanog pritiska na bubrege prevremeno rođene dece.
PB  - Bograd : Društvo za ishranu Srbije / Belgrade : Serbian Nutrition Society
T2  - Hrana i ishrana
T1  - The importance of mineral supply in preterm infant nutrition
T1  - Značaj minerala u ishrani prevremeno rođene odojčadi
VL  - 63
IS  - 1
SP  - 17
EP  - 25
DO  - 10.5937/hraIsh2201017L
ER  - 

@article{
author = "Lugonja, Nikoleta and Marinković, Vesna and Djurović, Dijana and Mileusnić Milenović, Radmila and Avdalović, Jelena and Vrvić, Miroslav",
year = "2022",
abstract = "Although human breast milk contains significant biomolecules
with major impact on growth and development of preterm babies, a diet based exclusively on this milk over a longer period of
time can be associated with the development of micronutrient
deficiency in this sensitive group of infants. The aim of this study
was to determine the content of micronutrients in human breast
milk from the mothers of preterm infants and in infant formula,
and the influence of mineral content on the osmolality and potential renal solute load. Human breast milk taken during the
lactation period was examined, before and after frozen storage,
pasteurization and supplementation with a fortifier, with mineral
content being determined by inductively coupled plasma-optical
emission spectrometry. Osmolality of preterm milk and infant formula was measured using an osmometer. During the lactation
period of mothers of preterm born babies, the mineral content
of the breast milk changed. The concentrations of all examined
minerals differed in colostrum and in mature milk. However, there
were no significant differences in mineral content in mature milk
before and after pasteurization and storage. Supplementation of
mature milk with a fortifier increased the concentration of minerals, the final osmolality and the potential renal solute load. The
mineral content of mature milk was lower than necessary for the
optimal growth of preterm infants, so adequate supplementation
with a fortifier is needed to provide biologically important minerals. However, the osmolarity of supplemented milk should be
monitored due to the potentially increased pressure on the kidneys of preterm infants, Iako humano mleko sadrži brojne biomolekule značajne za rast i razvoj prevremeno rođenih beba, ishrana bazirana isključivo na majčinom mleku tokom dužeg vremenskog perioda može biti povezana sa nedostatkom mikronutrijenata kod ove osetljive grupe odojčadi. Cilj ovog istraživanja je da se utvrdi sadržaj minerala u mleku majki prevremeno rođene dece i infant formuli, kao i njihov uticaj na osmolalitet, osmolaritet i potencijalno opterećenje bubrega rastvornim supstancama. Mineralne komponente infant formule i majčinog mleka u toku perioda laktacije, nakon skladištenja pasterizacije i suplementacije obogaćivačem, određene su primenom tehnike indukovano spregnute plazme - optičke emisione spektrometrije. Osmolalnost uzoraka mleka i infant formule je određena osmometrom. Tokom perioda laktacije majki prevremeno rođenih beba menja se sadržaj minerala u mleku. Koncentracije ispitivanih minerala su se razlikovale u zrelom mleku odnosu na kolostrum. Nije bilo značajne razlike u sadržaju minerala u zrelom mleku pre i nakon pasterizacije i zamrzavanja. Suplementacija zrelog mleka obogaćivačem povećava koncentraciju minerala, ukupnu osmolalnost i potencijalno opterećenje bubrega. Mineralni sadržaj zrelog mleka je manji od potrebnog za optimalan rast prevremeno rođene dece, stoga je neophodna adekvatna suplementacija obogaćivačem kojim se obezbeđuju biološki važni minerali. Međutim, potrebno je pratiti osmolarnost suplementiranog mleka zbog potencijalno povećanog pritiska na bubrege prevremeno rođene dece.",
publisher = "Bograd : Društvo za ishranu Srbije / Belgrade : Serbian Nutrition Society",
journal = "Hrana i ishrana",
title = "The importance of mineral supply in preterm infant nutrition, Značaj minerala u ishrani prevremeno rođene odojčadi",
volume = "63",
number = "1",
pages = "17-25",
doi = "10.5937/hraIsh2201017L"
}

Lugonja, N., Marinković, V., Djurović, D., Mileusnić Milenović, R., Avdalović, J.,& Vrvić, M.. (2022). The importance of mineral supply in preterm infant nutrition. in Hrana i ishrana
Bograd : Društvo za ishranu Srbije / Belgrade : Serbian Nutrition Society., 63(1), 17-25.
https://doi.org/10.5937/hraIsh2201017L

Lugonja N, Marinković V, Djurović D, Mileusnić Milenović R, Avdalović J, Vrvić M. The importance of mineral supply in preterm infant nutrition. in Hrana i ishrana. 2022;63(1):17-25.
doi:10.5937/hraIsh2201017L .

Lugonja, Nikoleta, Marinković, Vesna, Djurović, Dijana, Mileusnić Milenović, Radmila, Avdalović, Jelena, Vrvić, Miroslav, "The importance of mineral supply in preterm infant nutrition" in Hrana i ishrana, 63, no. 1 (2022):17-25,
https://doi.org/10.5937/hraIsh2201017L . .

TY  - JOUR
AU  - Ivanović, Ljubica
AU  - Topalović, Ana
AU  - Bogdanović, Višnja
AU  - Djurović, Dijana
AU  - Mugoša, Boban
AU  - Jadranin, Milka
AU  - Tešević, Vele
AU  - Beškoski, Vladimir
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4797
AB  - Purpose: The purpose of this paper is to determine the polyphenol content, antioxidative potential and antiproliferative activity of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. Design/methodology/approach: Swiss chard was grown in the open field (Lješkopolje, Montenegro) where it was subjected to different fertilization and irrigation regimes. Chard samples were analyzed for previously identified polyphenols and for antioxidant parameters. Additionally, in order to complete the biological activities, chard extracts were tested for antiproliferative activity against MFC-7 and HT-29 tumor cell lines. Findings: The polyphenols identified in Swiss chard were flavonoids: vitexin-2?-O-xyloside, vitexin-6?-O-acetyl-2?-O-xyloside, vitexin-6?-O-malonyl-2?-O-xyloside and isorhamnetin-3,7-diglucoside. In the antioxidant tests, DPPH (2,2-diphenyl-1-picrylhydrazyl), and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), chard extract had values of 7.00 and 8.50 (mean values) µmol Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equ/d.w., respectively. The tested chard extracts inhibited cell proliferation at different concentrations (3.125–50.0 µg/mL) against the MCF-7 cell line, after 24 and 48 h of incubation. The antiproliferative activity, expressed in terms of IC50, was 32.97 and 86.45 µg/mL after 24 h of incubation and 20.76 and 23.33 µg/mL after 48 h of incubation, for treated and untreated chard extracts, respectively. Originality/value: These data suggest Montenegrin Swiss chard grown under different irrigation and fertilization treatments can be considered as a functional food and should be included in an everyday diet. The collected data could help in the growth improvement of chard with functional food properties.
PB  - Emerald Publishing Limited
T2  - British Food Journal
T1  - Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes
VL  - 123
IS  - 7
SP  - 2335
EP  - 2348
DO  - 10.1108/BFJ-11-2020-1062
ER  - 

@article{
author = "Ivanović, Ljubica and Topalović, Ana and Bogdanović, Višnja and Djurović, Dijana and Mugoša, Boban and Jadranin, Milka and Tešević, Vele and Beškoski, Vladimir",
year = "2021",
abstract = "Purpose: The purpose of this paper is to determine the polyphenol content, antioxidative potential and antiproliferative activity of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. Design/methodology/approach: Swiss chard was grown in the open field (Lješkopolje, Montenegro) where it was subjected to different fertilization and irrigation regimes. Chard samples were analyzed for previously identified polyphenols and for antioxidant parameters. Additionally, in order to complete the biological activities, chard extracts were tested for antiproliferative activity against MFC-7 and HT-29 tumor cell lines. Findings: The polyphenols identified in Swiss chard were flavonoids: vitexin-2?-O-xyloside, vitexin-6?-O-acetyl-2?-O-xyloside, vitexin-6?-O-malonyl-2?-O-xyloside and isorhamnetin-3,7-diglucoside. In the antioxidant tests, DPPH (2,2-diphenyl-1-picrylhydrazyl), and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), chard extract had values of 7.00 and 8.50 (mean values) µmol Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equ/d.w., respectively. The tested chard extracts inhibited cell proliferation at different concentrations (3.125–50.0 µg/mL) against the MCF-7 cell line, after 24 and 48 h of incubation. The antiproliferative activity, expressed in terms of IC50, was 32.97 and 86.45 µg/mL after 24 h of incubation and 20.76 and 23.33 µg/mL after 48 h of incubation, for treated and untreated chard extracts, respectively. Originality/value: These data suggest Montenegrin Swiss chard grown under different irrigation and fertilization treatments can be considered as a functional food and should be included in an everyday diet. The collected data could help in the growth improvement of chard with functional food properties.",
publisher = "Emerald Publishing Limited",
journal = "British Food Journal",
title = "Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes",
volume = "123",
number = "7",
pages = "2335-2348",
doi = "10.1108/BFJ-11-2020-1062"
}

Ivanović, L., Topalović, A., Bogdanović, V., Djurović, D., Mugoša, B., Jadranin, M., Tešević, V.,& Beškoski, V.. (2021). Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. in British Food Journal
Emerald Publishing Limited., 123(7), 2335-2348.
https://doi.org/10.1108/BFJ-11-2020-1062

Ivanović L, Topalović A, Bogdanović V, Djurović D, Mugoša B, Jadranin M, Tešević V, Beškoski V. Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. in British Food Journal. 2021;123(7):2335-2348.
doi:10.1108/BFJ-11-2020-1062 .

Ivanović, Ljubica, Topalović, Ana, Bogdanović, Višnja, Djurović, Dijana, Mugoša, Boban, Jadranin, Milka, Tešević, Vele, Beškoski, Vladimir, "Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes" in British Food Journal, 123, no. 7 (2021):2335-2348,
https://doi.org/10.1108/BFJ-11-2020-1062 . .